1 Laboratory Techniques in Exercise Physiology Lab 1 House Rules and Laboratory Safety House Rules 1.

Clean-up the lab when you are finished. If you don't have time to clean up after the lab you don't have time to work. Leave the laboratory clean, return all equipment and chemicals back to its proper location, and wash glassware. Remember you are not the only one working in the lab. 2. Report all damaged or broken equipment immediately to the lab instructor. Failure to do so will impede the progress of other experiments in the lab and can become hazardous to other users. 3. We have a limited amount of supplies. Please conserve disposable supplies when possible, prevent contamination of stock reagents and never open new containers without the consent of the lab instructor. 4. Be courteous of others, keep music and conversation at low levels and clean-up when you are finished. 5. Unauthorized experiments are not allowed. 6. You may use lab equipment only after you have been checked out on the proper use of the equipment by the lab instructor. 7. Be alert to unsafe conditions and actions and call attention to them so that corrections can be made as soon as possible. Someone elses accident can put you in danger. 8. Avoid distracting or startling any other worker. Practical jokes or horseplay have no place in the lab. 9. Think, act, and encourage safety until it becomes a habit.

2 Laboratory Safety Guidelines Rules of laboratory safety and chemical handling are not designed to impede productivity, nor should they instill a fear of chemicals or laboratory procedures. Rather, their purpose is to create a healthy awareness of potential laboratory hazards, to improve the efficiency of each student worker, and to protect the general public and the environment from waste contamination. Laboratory Safety Rules: 1. Lab coats should be worn at all times. 2. Some form of eye protection should be worn at all times. Eye injuries can be very serious and injury is usually nonreversible. Safety glasses with wide side shields are recommended, but normal eyeglasses with safety lenses may be permitted. Contact lenses can be problematic while working in the laboratory. The major problem with contact lenses is that they reduce the rate of self-cleansing of the eye. If a chemical splatters into the eye of a contact lens wearer, he or she will possibly have greater injury because of less effective irrigation of the eye. Furthermore, the wearer may be reluctant to use an eyewash for fear of losing the contact lens. 3. Never work alone in the laboratory. 4. Be familiar with the physical properties of all chemicals used in the laboratory. This includes their flammability, reactivity, toxicity, and proper disposal. 5. Eating, drinking, and smoking in the laboratory are strictly prohibited. 6. Use the fume hood when working with any toxic or volatile chemical. 7. Know the location and proper use of the fire extinguishers, eye wash station, and deluge/shower. 8. Wash hands thoroughly before leaving the lab. 9. Use of disposable gloves is mandatory when working with any human blood or tissues. 10. Mouth pipetting is never allowed. 11. Report any spill, accident, or injury, no matter how minor to the lab instructor immediately.

3 Lab Assignments 1. Complete the online training course on lab safety (link is provided below). Print the last page of the course as verification that you completed the course. When asked , select researcher and cell biology. It will take you 45-60 minutes to complete the course. http://www.hhmi.org/about/harvest/labsafe/safescience.html 2. Complete the lab safety questionnaire. Due Date: Sept 7th

4 Laboratory Techniques in Exercise Physiology Lab Safety Questionnaire Exercise Biology Laboratories 1. Number and location of fire extinguishers. List the last inspection date and the type of fire extinguisher. List the proper procedures for using a fire extinguisher.

2. Location of telephone and emergency procedures

3. Number and location of spill clean-up kits (blood and chemical). Familiarize your self with the procedures for their use.

4. Number and location of first aide kits. List the contents of the first aide kits. Do the first aide kits appear to be complete?

5 5. Locations of safety showers and eyewash stations. When should they be used? Have they be flushed and cleaned regularly to minimize microbial contamination?

6. Location and type (safety glasses with full side shields, safety chemical goggles, plastic face shields) of personal eye protection. When should these be used?

7. Location of appropriate rubber gloves and other safety equipment for handling hazardous chemicals.

8. Location of appropriate rubber gloves for handling biohazardous materials.

9. Location of hot plates

10. Identify electrical outlets without ground, with ground, and with ground fault interrupter.

6 11. Location of compressed gas cylinders. Are they properly secured?

12. Location of waste receptacles for glass. 13. Location of waste receptacles for hazardous chemicals.

14. Location, and type of special cabinets to store hazardous or flammable chemicals. 15. List the chemicals found in the special cabinets. Are the chemicals found in the chemical cabinets appropriate for the cabinet?

16.Describe the OSHA labeling system for chemicals. When should the labels be checked?

7 17. Identify the location of the fume hood. What type of chemicals should be pipetted under the fume hood? Describe its proper use.

18. Identify the location of the biological safety cabinet. When should it be used? When was it last certified?

19. Are exits properly marked? 20. Location of the MSDS notebook. Describe the contents of this notebook. What is the purpose of the notebook and when should it be consulted?

21. Location of the master cut-off switch for electricity

22. Location of biohazardous bags (empty and active) and boxes. When and where should the used biohazard bags be stored? Describe the procedures for handling biohazardous waste.

8 25. Location of sharps containers. What should be included in these containers? When and where should the used sharps containers be stored?

26. What type of materials should go into a biohazardous bag and into a sharps container?

27.Identify the powdered chemical cabinet. How can you determine if the chemicals in this cabinet are hazardous and whether special precautions should be taken when handling these chemicals?

28. Location of the fire alarm.

29. Suggestions for improving the safety of the Laboratory. What safety equipment would you purchase for the lab? Why?