R&D LEADERSHIP: ASSOCIATE SCIENTIST, RESEARCH ASSOCIATE
Pharmaceuticals and Biotechnology, Research and Formulations Innovative Associate Scientist with more than 20 years of experience delivering ground-breaking research for world-class organizations including Fortune 500 leaders XXX. Conduct cutting-edge pharmaceutical and biotech research with Subject Matter Expertise in lipid-based therapeutic agent development. Possess hands-on laboratory skills across biochemical/chemical assay development, cell cultures, liposomes, chromatography, protein characterization, DNA analysis, and spectroscopy. Expertise in advanced research methods, co-authoring publications in Bioconjungate Chemistry and Experimental Cell Research. Versatile skill base includes ISO 14001 standards, design of experiments (DOE), feasibility studies, and patent protection with fluent French and Greek language skills. Recognized as a skilled problem solver, articulate communicator, and respected team player with impeccable interpersonal skills.
Research & Development • DNA Analysis • Chromatography • Protein Characterization
Design of Experiments (DOE) • Good Manufacturing Practices (GMP) • Spectroscopy Biochemical/Chemical Assays • Stability Studies • Quality Control (QC) Dynamic Light Scattering (DLS) • LAL Gel Clot Assay • In Vitro Release Assays
PROFESSIONAL EXPERIENCE
Career Note: Completed training/coursework from 2007-Present.
COMPANY, Location • 1999 to 2007
Global pharmaceutical leader introducing oral, transderal, implantable, and liposomal products. Associate Scientist, Intravenous Technology Development (2001 to 2007) Instrumental to Formulations and Research group success, developing key Active Pharmaceutical Ingredient (API) biochemical/chemical assays. Hands-on experience included small-scale liposome production formulating compounds into Stealth liposomes, Pre-IND level drug formulation, and lipid-based small molecule and protein products for oncology and infectious disease studies. Served as key liaison between formulation, QC, and pharmacology teams. Conducted stability, toxicity, and efficacy studies. Complied with ISO 14001 guidelines. Invented in vitro release assay predicting formulation stability, boosting productivity via standard fluorimeter-based assay system, testing numerous disruptive agents, and discerning correlation between in vitro release assay and in vivo PK rat data. Positioned product as best-in-class, creating formulation variants and protecting patent position. Launched system targeting HIV-infected cells via antibody fragments conjugated to liposome surface and created in vitro liposome uptake assay for formulation.
Chemist III (1999 to 2001)
Served as key member of pharmacokinetic testing team, formulating liquid liposome-based products for testing. Strengthened bench and pilot scale processes via DOE methodologies. Directed sterile filtration validation tests and critical stability studies. Created small-scale production equipment cleaning procedures. Led targeted liposome technology extramural program activities. Established optimal stability conditions for liquid liposome-based products via DOE methods. Introduced clinical protocol condition simulation, illustrating drug stability for up to 72 hours. Developed potential resolutions to Stealth-Radiation sensitizer product pyrogen issue.
COMPANY, Location • 1992 to 1999
Pharmaceutical firm utilizing liposomes as drug delivery carriers. Research Associate II Created critical biochemical/chemical assays and formulations of interest. Documented research and developed manufacturing processes complying with GMP guidelines. Facilitated insightful formulation stability studies, planning and executing key research. Resume NAME • Page 2 • Email
ADDITIONAL EXPERIENCE
INSTITUTE, Location Research Associate, Laboratory of Gene Regulation/Metastasis Orchestrated cancer metastasis mechanism elucidation research utilizing primary and metastatic tumor cell lines (B16 mouse melanoma: F1, F10, and F15) and protein profiling methodologies. Uncovered primary and metastatic cell protein differences. Originated optimal protein profiling assay, advancing assay to ovarian cancer cells.
UNIVERSITY, Location Research Associate, Laboratory of Monoclonal Antibody Conducted critical research and analysis for Faculty of Medicine and Department of Biochemistry. Studied role of (ADP-ribosyl)ation in chromatin structure and carcinogenesis. Researched systemic lupus erythromatosus/rheumatoid arthritis patient treatment, analyzing histones (ADP-ribosyl)ation using cells in culture and controlling eukaryotic cell gene expression.
Note: Experience includes Laboratory Assistant role with Research Institute. Details provided on request.
FORMAL EDUCATION
Bachelor of Science, Biology
University, Location
Paramedical Biochemistry Coursework
University, Location
Diploma, French Language
University, Location
PROFESSIONAL DEVELOPMENT
HPLC Coursework • Design of Experiments Coursework
Chromatography: SEC, IEX, Affinity • DNA Analysis: Restriction digest, AUT, Agarose gel electrophoresis Protein characterization: SDS-PAGE, IEF, NEPHGE, Protein assay, Protein isolation and purification, 125 35 Radiolabelling ( I, S) and conjugation to polymer Spectroscopy: AA, UV-VIS and Fluorescence Cell culture (monolayer/suspension) • Cytotoxicity assay • Membrane isolation • Nuclei isolation LAL gel clot assay • Radiolabelling of liposomes (67Ga) • Fluorescent labelling of liposomes • DLS Autoradiography • GLP/GMP manufacturing • Process optimization • Scale-up • Technology transfer In vivo and in vitro assays Resume
