New Biotechnology Volume 25S September 2009

ABSTRACTS

3.2.10
Bioelicitation of secondary metabolites in in vitro cultures of Gymnema sylvestre R.Br. C. Subathra Devi , V. Mohana Srinivasan
VIT University, Vellore, India

3.2.12
Expression and functional analysis of rice-produced human insulin-like growth factor-I (hIGF-I) S.C.K. Cheung 1, , L. Liu 1 , S.S.M. Sun 1 , Q. Liu 2 , L. Lan 1 , J.C.N. Chan 1 , P.C.Y. Tong 1
1

Aspergillus niger cell extract was used as a elicitor. Growth rate and the gymnemic acid production of the elicited and non-elicited cultures of Gymnema sylvestre were investigated. The growth rate of the elicited and non-elicited cultures was not signicantly different, but the gymnemic acid yield was very high in elicited cultures. When compared to non-elicited cultures, 89-fold increase of gymnemic acids yield in elicited cultures was noticed. 10 g of cell suspension produced in shake ask culture was transferred to 5 l batch fermentor containing 2-l MS medium. Fresh weight was increased 6.5 times after 20 days of culture and the gymnemic acid production achieved was 900 mg/l. After elicitation, 8 times increase of fresh weight was gained. When compared to shake ask culture, 89-fold increase in gymnemic acid production was noticed in fermentors. doi:10.1016/j.nbt.2009.06.643

The Chinese University of Hong Kong, Hong Kong Yangzhou University, Hong Kong

3.2.11
Spirulina as functional food M. Ajeesh , C.P.N. Bohra, N. Gupta, C. Rajasekaran
Plant Biotechnology Division, School of Biotechnology, Chemical and Biomedical Engineering, VIT University, Vellore 632014, India

Functional foods are foods that contain multiple ingredient/s and contribute constructive health benets over other forms of food. They can be food with or enriched with bioactive substances or traditional food with synthesized food ingredients. Spirulina, a lamentous cyanobacterium, with high nutritive value has been under immense research for its role in medication and as well as in Nutraceuticals. Besides inhibitory effect on viral replication and ageing mechanism, Spirulina has been found to have detoxication of undesirable compounds and help reduce oxidative damage done by free radicals in the body. This poster focuses the use of Spirulina as a functional food in our diet. It also accentuates the role of Spirulina metabolites such as amino acid, pigments, vitamins and minerals in imparting improved metabolism and body functions. doi:10.1016/j.nbt.2009.06.644

Human insulin-like growth factor-I (hIGF-I) is a growth factor that highly resembles to insulin. It is essential for cell proliferation and can be used to treat various endocrine-associated diseases including growth hormone insensitivity syndrome (GHIS) and diabetes mellitus. To meet the increasing demand of hIGF-I in therapeutic uses, we have developed an efcient plant expression system to produce biologically active recombinant hIGF-I (rhIGF-I) in transgenic rice grains. The plant-codon-optimized hIGF-I, under the control of rice seed-specic Gt1 promoter, was introduced into rice via Agrobacterium-mediated transformation. In order to enhance rhIGF-I stability and yield, two protein-sorting sequences, an endoplasmic reticulum (ER)-retention signal (KDEL) and glutelin signal peptide, were used to deliver the recombinant protein to ER for stable accumulation. In vitro functional analysis showed that the rice-derived rhIGF-I could induce membrane rufing and glucose uptake on L6myc cells in a dose-dependent manner. It was estimated that 1 mg of transgenic rice grain contained equivalent biological activity of 1.63.2 nM of commercial rhIGF-I. In vivo oral meal test with rice-derived rhIGF-I was also found to acutely lower blood glucose level in normal C57/BL6 mice. The blood glucose levels of the mice fed with wild type rice were always higher than those fed with rhIGF-I rice throughout the experiment (p < 0.05). Both of the blood glucose levels reached maximum at 15 minutes, with 3.42 0.76 and 2.49 0.35-fold for wild type and rhIGF-I rice respectively (p < 0.01), indicating that rhIGF-I rice was more effective in lowering blood glucose than wild type rice. To conclude, our ndings provided an alternative expression system to produce large quantities of functional rhIGF-I that can be a promising substitution of insulin for diabetic treatment. doi:10.1016/j.nbt.2009.06.645

3.2.13
Biopharmaceutical potential of submerge cultivated higher basidial mushrooms Mark Shamtsyan , Vera Kolesnikov, Anton Klepikov Spiridonova, Eugenia Tozik, Boris

St. Petersburg State Institute of Technology (Technical University), St. Petersburg, Russian Federation

From ancient times humans have used mushrooms for their healing potential. The major part of the traditional knowledge of mushrooms bioactive properties has originated from the Oriental countries. Western countries are just discovering the variability and tremendous potential of medical mushrooms.

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