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Table of Contents

Sales, Service, and Support


Technical Support Information . . . . . . . . . . . . . . . .3 Training Information . . . . . . . . . . . . . . . . . . . . . . . .4

37
Services Information . . . . . . . . . . . . . . . . . . . . .45 Agilent Offices . . . . . . . . . . . . . . . . . . . . . . . . . .67

Featured Products General Chromatography


Vials, Caps and Septa . . . . . . . . . . . . . . . . . .1238 Syringes . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3946 Gas Management . . . . . . . . . . . . . . . . . . . . .4782 Hardware . . . . . . . . . . . . . . . . . . . . . . . . . . .8391

9 11109
Standards . . . . . . . . . . . . . . . . . . . . . . . . . .92101 Integrators, Integrator Supplies and Cables . .102106 Books, Learning Software and CDs . . . . . .107109

Sample Preparation
Solid Phase Extraction (SPE) . . . . . . . . . . .111131 Manifolds and Accessories . . . . . . . . . . . .132138

111147
CombiZorb Scavengers . . . . . . . . . . . . . . .139141 Sample Filtration . . . . . . . . . . . . . . . . . . . .142147

GC and GC/MS
GC Supplies . . . . . . . . . . . . . . . . . . . . . . . .149168 GC Instrument Parts and Supplies . . . . . . .169198 GC/MS Parts and Supplies . . . . . . . . . . . . .199216

149441
GC and GC/MS Columns . . . . . . . . . . . . . .217323 Troubleshooting/Maintenance . . . . . . . . . .324340 Applications . . . . . . . . . . . . . . . . . . . . . . . .341441

LC and LC/MS
LC Supplies . . . . . . . . . . . . . . . . . . . . . . . .443456 LC Instrument Parts and Supplies . . . . . . . .457500 LC/MS Parts and Supplies . . . . . . . . . . . . .483484

443652
LC and LC/MS Columns . . . . . . . . . . . . . . .501599 Troubleshooting/Maintenance . . . . . . . . . .600603 Applications . . . . . . . . . . . . . . . . . . . . . . . .604652

CE and CE/MS
CE Supplies . . . . . . . . . . . . . . . . . . . . . . . .653654 CE Instrument Parts and Supplies . . . . . . . . . . . .655 CE/MS Instrument Parts and Supplies . . . .656659

653668
Capillaries . . . . . . . . . . . . . . . . . . . . . . . . .660666 Solution Kits . . . . . . . . . . . . . . . . . . . . . . .666668

ICP-MS
7500 ICP-MS Supplies . . . . . . . . . . . . . . . .669678 4500 ICP-MS Supplies . . . . . . . . . . . . . . . .679688

669690
ICP-MS Standards . . . . . . . . . . . . . . . . . . .689690

UV-Vis
Cells and Cell Supplies . . . . . . . . . . . . . . . .691694 Tubing and Fittings . . . . . . . . . . . . . . . . . . . . . . .695 Spectrophotometer Supplies . . . . . . . . . . .696697

691699
Dissolution Testing Supplies . . . . . . . . . . . . . . . .698 Chemical Standards and Accessory Kits . . . . . . .699

Indices
Alphabetical . . . . . . . . . . . . . . . . . . . . . . .701707 Compound . . . . . . . . . . . . . . . . . . . . . . . . .708719

700744
Part Number . . . . . . . . . . . . . . . . . . . . . . .720744

Ordering Information
Easy Ordering Terms and Conditions . . . . . .745746 Custom Column Orders . . . . . . . . . . . . . . .746748

745752
Easy Ways to Order . . . . . . . . . . . . . . . . . .748749 Order Forms . . . . . . . . . . . . . . . . . . . . . . .750752

Information in this document is subject to change without notice.

Agilent Technologies reserves the right to correct any printing or typographical error in this catalog.

Prices in this catalog are for reference only and are subject to change without notice.

Application Information in this catalog is for investigational use only and is not intended to be a complete laboratory diagnostic procedure or method. The performance characteristics of the procedures have not been fully established. The choice of methodology and procedures remains the responsibility of the user and not of Agilent Technologies.

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Technical Support Information Technical Support at Work for You


You're in the lab and you're busy and something isn't right. When you have a column or supplies technical question, you want the answer NOW. Our Technical Support Specialists are ready to answer your call. These chemists specialize in working together with you to provide the answers you need to keep your laboratory productivity high. Fast answers to your column or supplies technical questions are available from Agilent: 1. Questions pertaining to supplies found in this catalog, call 1-800-227-9770 in the U.S. and Canada or call your local Agilent sales office. Give a brief overview of your questions or dilemma, and a technical expert for that product or process will be right with you. All of our technical support specialists are chemists with years of laboratory experience. They can provide you with in-depth knowledge and experience in consumables and accessories product use and applications. 2. Column selection questions, in the U.S. and Canada, call 1-800-227-9770 and select option 4, 3. Technical Support on the Internet, (Agilent provides world class technical support online at www.agilent.com/chemvoted Best Laboratory Products Manufacturer website in an independent survey conducted at PITTCON.) As the industry leader, Agilent Technologies believes in sharing its wealth of knowledge and information with industry professionals to help you work more effectively and efficiently.

Sales, Service and Support

Instrument Repair Parts Questions


Instrument repair parts are not, for the most part, listed in this catalog. Rather, they are identified in Agilent user or service manuals. If you have questions about instrument repair or require instrument repair information, call 1-800-227-9770, option 3, in the U.S. and Canada. Instrument repair parts are available from the Agilent Support Materials Organization. In the U.S., call 1-877-447-7278 for overnight shipment of repair parts. For repair parts outside the U.S., call your authorized Agilent Technologies distributor or contact your local authorized Agilent service representative.

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Training/Services Information Chemical Analysis Training Programs


Sales, Service and Support
Agilent Technologies provides carefully planned, effective training courses in using and maintaining Agilent chemical analysis hardware and software products. You can obtain information, see course schedules and register by: Logging on to www.agilent.com/chem and go to the Education link under chemical/biochemical Calling toll-free: 1-800-227-9770 in the U.S. and Canada, or Contacting you Agilent sales representative

Service Bundles Meet Specific Needs for Less Cost


Support Services for Agilent Instruments
Special service bundles contain essential services to keep your chemical analysis instruments in top condition for dependable operation. These bundles cost less than purchasing each service individually.

Petrochemical Bundle
For the specific needs of the petroleum, chemical, petrochemical and other industries not subject to pharmaceutical regulatory compliance, this service bundle provides: Telephone Support to Isolate and Resolve Hardware ProblemsIncluded Without Charge At-Your-Site Service with Consumables used during Repair All Off-Site Instrument Repair Services Annual On-Site Preventive Maintenance

Pharmaceutical Bundle
For the pharmaceutical, agricultural and other industries that must comply with rigorous quality and regulatory requirements, this service bundle provides: Telephone Support to Isolate and Resolve Hardware ProblemsIncluded Without Charge At-Your-Site Service with Consumables used during Repair Annual On-Site Preventive Maintenance Annual Operational Qualification/Performance Verification

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Services Information Configure Your Own Support Package


Support Services for Agilent Instruments
You can protect your investment by selecting from a menu of Agilent support services. You can purchase only what you need, for maximum flexibility in meeting your laboratory performance and budget objectives.
Agilent Support Service Repair At-Your-Site Instrument Repair Features Telephone support, with access to highly trained technical support professionals Travel expenses and labor cost for customer service professional Parts for on-site repair A choice of response times Optional Coverage Consumables used during repair Option 1 Replacement with identical instrument the fastest way to resolve a problem Option 2 Return to Agilent for repair Expert cleaning, adjusting, lubricating, or inspecting Travel and labor costs included A predefined checklist of preventive maintenance procedures for consistent service from laboratory to laboratory On-site disassembly Cleaning Re-assembling Testing Advantages to You Fixed annual cost for parts, allowing predictable budget management Selection of response times to suit your uptime and budget requirements

Sales, Service and Support

Off-Site Instrument Repair*

Lower-cost support than on-site repair Extensive diagnostic and testing that are not feasible with on-site maintenance or repair

Maintenance Preventive Maintenance*

Maximized instrument uptime Extended instrument life Confidence in the integrity of your measurements

Mass Spectrometer Ion Source Cleaning*

Laboratory staff can devote more time to chemistry, not instrument cleaning Efficient cleaning for minimum downtime The evidence needed to help you satisfy regulatory agencies No need to write your own standard operating procedures to qualify Agilent instruments and train your staff Consistent and traceable results among all your laboratories and improved method transfer Confidence in the integrity of your measurements, with traceable, documented chains from sample introduction to reporting Fast problem identification and resolution Fixed annual cost for software updates, for predictable budget management Timely and convenient way to remain current with the latest features, enhancing laboratory capability Cost-effective coverage for additional, identical systems at a single location
*For selected Agilent instruments

Qualification Operational Qualification/ Performance Verification*

Verification and documentation of an instruments ability to meet specified criteria Procedures and documentation that fit the requirements of GLP, ISO 9000, and other regulatory agencies Measuring equipment traceable to national and international standards Preventive maintenance is recommended before Operational Qualification/Performance Verification Telephone assistance from highly trained technical professionals to isolate and resolve software problems All enhancements to the original chemical analysis application software purchase Agilent software status bulletins that identify discovered defects and recommend workarounds

Software

Telephone Support and Updates for Software

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Agilent Offices Worldwide


Argentina
Agilent Technologies Av. Crdoba 1131 (C1055AAB) Capital Federal, Argentina Phone: 5811-7260 Fax: 5811-7126 analitica@agilent.com

China
Agilent Technologies Co. Ltd (China) 15F, Hua Xin International Building 219 Qingnian Street Shenyang 110015, Liaoning Province P R China 100022 Phone: 86-24-23-964688 Fax: 86-24-23-964788 Agilent Technologies Co. Ltd (China) HP Building, 8th Floor No. 2 Dong San Huan Nan Lu Chao Yang District, Beijing 100022 P R China Phone: 8610-6564-5513 Fax: 8610-6566-8330 Agilent Technologies Co. Ltd (China) Da Ye Building, 22nd Floor No. 39 Da Ya Road Chengdu 610016 P R China Phone: 86-28-665-5500 Fax: 86-28-667-4321 Agilent Technologies Co. Ltd (China) Unit 08, 66F Citic Plaza 233 Tian He Bei Road Guangzhou 510613 P R China Phone: 86-20-8668-5500 Fax: 86-20-8669-5861 Agilent Technologies Co. Ltd (China) Level 12 Novel Building 887 Huai Hai Zhong Lu Shanghai 200020 P R China Phone: 86-21-6474-5500 Fax: 86-21-6474-8307

Finland
Agilent Technologies Finland Oy Linnoitustie 2 B P.O. Box 81 02601 Espoo Phone: 01080 2220 Fax: 010 855 2667

Japan
Yokogawa Analytical Systems Inc. 1-15-5 Nakacho, Musashino-shi Tokyo 180 8543 Phone: 0120-477-111

Sales, Service and Support

Korea
Agilent Technologies Korea Ltd 25-12, Yoido-dong Youngdeungpo-gu Chemical Analysis Group (CAG) Unit no 201, level 2, Uptown 2 2 Jalan SS 21/37, Damansara Uptown 47400 Petaling Seoul 150-010 Phone: 822-2004-5096 Fax: 822-2004-5089

Australia
Agilent Technologies Australia Pty Ltd Unit 12/2 Eden Park Drive Forest Hill North Ryde NSW 2113 Phone: 800-802402 Fax: 612-9805-6301

France
Agilent Technologies Groupe Analyse Chimique 1 rue Galvani F-91745 MASSY Cedex N AZUR : 0810 446 446 Fax : 0825 010 708 csc-france_analyse-chimique@ agilent.com

Austria
Agilent Technologies sterreich GmbH Chemische Analysentechnik Dresdner Str. 81-85 1200 Wien Phone: (01) 25125-6800 Fax: (01) 25125-7365 analytik_austria@agilent.com www.agilent.de

Germany
Agilent Technologies Deutschland GmbH Chemische Analysentechnik Hewlett-Packard-Str. 8 76337 Waldbronn Phone: 0800-603 1000 Fax: 07243-602602 analytik_germany@agilent.com www.agilent.de

Malaysia
Agilent Technologies Sales (M) Sdn Bhd Jaya Selangor Darul Ehsan West Malaysia Phone: 603-1-800-88-0805 Fax: 603-7727-1239

Netherlands
Agilent Technologies Netherlands B.V. Startbaan 16 1187 XR Amstelveen Phone: 020 547 2600 Fax: 020 547 2690 chem_benelux@agilent.com

Belgium
Agilent Technologies Belgium SA/NV Boulevard De La Woluwe 100 1200 Brussels Phone: +32 (0)2-4049222 Fax: +32 (0)2-4049290 cag_belgium@agilent.com

Hong Kong
Agilent Technologies Hong Kong Ltd 24th Floor, Cityplaza One 1111 King's Road Taikoo Shing Phone: 852-3197-7341 Fax: 852-2506-9275

Norway
Agilent Technologies Norway AS Drammensveien 169 0277 OSLO Phone: 815 32 560 Fax: 2325 3710 kjemisk_analyse@agilent.com www.agilent.com/chem

Brazil
Agilent Technologies Brasil Al. Araguaia, 1142 - 1o Andar Alphaville 06455-940 Barueri - SP Phone: 0800-781405 Fax: (11)7297-3943 vendas_cag@agilent.com

India
Agilent Technologies India Pvt. Ltd Chandiwala Estate Maa Anandmai Marg Kalkaji, New Delhi 110 019 Phone: 91-11-682-6067 Fax: 91-11-682-6068

Denmark
Agilent Technologies A/S Nrum Hovedgade 8 2850 Nrum Phone: +45 7013 0020 Fax: +45 4582 0630 susanne_knudsen@agilent.com

Canada
Agilent Technologies Canada Inc. 5150 Spectrum Way Mississauga, ON L4W5G1 Phone: 800-265-7768 Fax: 514-695-0259 Agilent Technologies Canada Inc. Chemical Analysis Group 2660 Matheson Blvd. E. Mississauga, ON L4W5M2 Phone: 514-693-3155 Fax: 514-693-3170 CAGMTKG@agilent.com

Ireland
Agilent Technologies Ireland Ltd. Silverstone House Ballymoss Road Sandyford Dublin 18 Phone: 01 605 8324 Fax: 01 605 8323 cag_enquiry@agilent.com

Poland
Agilent Technologies Polska Ul. Pulawska 303 02-785 Warszawa Phone: +48 22 549 1400 Fax: +48 22 549 1401 klient@agilent.com

Dominican Republic
Agilent Technologies de Venezuela C.A Av. Francisco de Miranda Torre HP, Piso 12 Los Palos Grandes Caracas, Venezuela CP 1060 Phone: (58212)-209-4613 Fax: (58212)-209-4618 arelis_guerrero@agilent.com

Singapore
Agilent Technologies Singapore (Sales) Pte Ltd Blk B #05-12 Alexandra Technopark 438-B Alexandra Road Singapore 119968 Phone: 65-215-6316 Fax: 65-271 0423

Italy
Agilent Technologies Italia S.p.A. Phone: 800 01 2575 (numero verde) Fax: 02-9210 4575 analitica_italia@agilent.com

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Agilent Offices Worldwide


Spain
Agilent Technologies Spain, S.L. Grupo de Anlisis Qumico Ctra. N-VI, Km 18,200 28230 Las Rozas, Madrid Phone: 901 11 68 90 Fax: 34 916 376 505 cag_instalaciones@agilent.com

United Kingdom
Agilent Technologies UK Limited Lakeside Cheadle Royal Business Park Stockport Cheshire SK8 3GR Phone: 0845 712 5292 Fax: 0161 492 7171 cag_enquiry@agilent.com

Sales, Service and Support

Sweden
Agilent Technologies Sweden AB, Box 52, 164 94 Kista Phone: 08 506 489 50 Fax: 08 506 489 51

United States
Agilent Technologies, Chemical Analysis Group North American Sales, Customer Contact Center, 2850 Centerville Road Wilmington, Delaware 19808 Phone: 800-227-9770 Fax: 302-633-8901 cag_sales-na@agilent.com www.agilent.com/chem

Switzerland
Agilent Technologies (Schweiz AG) Chemische Analysentechnik In den Luberzen 29 8902 Urdorf Phone: 0848 803 560 Fax: 01 735 97 44 cag_qualify@agilent.com www.agilent.ch

Venezuela
Agilent Technologies de Venezuela C.A Av. Francisco de Miranda Torre HP, Piso 12 Caracas, CP 01060 Phone: (58212)-209-4613 Fax: (58212)-209-4618 vilma_bastidas@agilent.com

Taiwan
Agilent Technologies Taiwan Ltd 13th Floor, 10 Syh-Wei, 4th Road Kaohsiung 802 Phone: 886-7-337-7631 Fax: 886-7-536-2595 Agilent Technologies Taiwan Ltd 8F, No. 2, Sec. 1, Fushing S. Road Taipei 104 Taiwan Republic of China Phone: 886-2-2734-5686 Fax: 886-2-8773-9003 ling_yu@agilent.com

Vietnam
Agilent Technologies (Vietnam) Ltd Unit 503-504, 5th Floor 30 Nguyen Du Hai Ba Trung District Phone: 844-943-0701/4 Fax: 844-943-0703

Thailand
Agilent Technologies (Thailand) Ltd Vibulthani Tower 11, 23-25th Floor 3199 Rama IV Road Klong Tan, Klong Toey, Bangkok 10110 Phone: 662-661-3900 Ext 3103 Fax: 662-661-3941 Agilent Technologies (Thailand) Ltd 267/42 Sukhumvit Road, Tambol Map Ta Phut, Amphur Muang Rayong 21150 Phone: 662-38-607708 Fax: 662-38-607710

Note: For a complete listing of authorized distributors, visit our website www.agilent.com/chem or contact your nearest Agilent office.

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Web Information
Have you been to the Agilent Chemical Analysis Web Site lately?
Go to www.agilent.com/chem and find what you need, when you need it: Online ordering: instruments, supplies, and software Product availability Customer-specific product pricing Quick Buy feature makes repeat purchases easy and fast Order status and tracking Order history Save and retrieve quotes Frequently Asked Questions: Answers to the most common technical questions about our products Technical Support Assistant: A logical step-by-step approach to help you solve common problems Technical Document Library: A searchable collection of service and support documents GC, LC, and CE Reference Chromatogram Libraries: A searchable collection of nearly a thousand chemical compounds Operating manuals, installation guides Illustrated Parts Breakdowns: Instrument diagrams to help you identify a part or find a part number Software Status Bulletins: Known software defects, work-arounds, and patches

And it is growing every day!

Register on our website today and sign up for our monthly email newsletter to keep up with the new features as they are added to our #1 rated website.

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Featured Products Demonstrating the Value of Deep Knowledge


Were calling them Featured Products , but they are much more than that. They represent the whole new approach that Agilent is taking to its business. We realize that you dont just need the highest quality instruments, columns, and supplies; you need a partner who can bring you solutions based on long experience and expert insight. Thats what each of the products in this section represents. From an item as specific as a patented new vial for extreme recovery, to the comprehensive information we pack into our website, each is a way for Agilent to share 35 years of daily work by hundreds of technical experts in your application areaon the instruments you use. Better still, each product is designed to make your job easier and your lab more productive, which is what a true partnership is all about.

Featured Products

Zorbax SPE Products

2002 Featured Products


New 96 well plates and cover mats for well plate autosamplers: Sample Preparation Section, page 113 Micro-V economical microsampling vials for Agilent autosamplers: General Chromatography, pages 16, 21, 25 Zorbax Solid Phase Extraction sample preparation products: Sample Preparation, page 114 AccuBONDII SPE cartridges in a wide variety of phases: Sample Preparation, page 120 Extreme Recovery 1.5 mL crimp cap vials: General Chromatography, page 22 Interseal caps with bonded septa for 2 mL screw top vials: General Chromatography, page 17 Electronic crimpers and decappers for 2 mL and 20 mm vials: General Chromatography, page 37 Expanded offering of gas purification devices, including the Big Universal Trap: General Chromatography, page 47 Comprehensive line of flowmeters from the classic digital bubble meters to combination flowmeter/leak detector, the FlowTracker 2000: General Chromatography, page 73 The new consolidated J&W Scientific brand of GC columns: GC and GC/MS Section, pages 217323 Poroshell particle design HPLC columns for high velocity and high resolution of protein separations: LC and LC/MS Section, pages 535536 Gel filtration chromatography columns from Shodex: LC and LC/MS Section, page 586587 Zorbax SB-Aq Rapid Resolution LC columns for polar analytes: LC and LC/MS Section, page 530531 100 mm LC columns: LC and LC/MS Column Section Agilent MicroBore and Capillary LC columns: LC and LC/MS Section, pages 560561 Zorbax 300Extend LC columns for excellent resolution and long lasting stability from pH 2 to 11.5: LC and LC/MS Section, pages 547559 Agilent Chemical Analysis Website: www.agilent.com/chem

Screw caps with Integrated Septa

Electronic Crimper and Decapper

GC Capillary Column

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General Chromatography
Table of Contents
General Chromatography Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11 Vials, Caps and Septa . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12 Septum Selection Guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13 Vial Identification Chart . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14 Screw Top Vials, Caps, Septa and Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1519 Crimp Top Vials, Caps and Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2023 Snap Top Vials, Caps and Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2427 Wide Opening Inserts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2728 8-425 Screw Top Vials, Caps, Septa and Inserts . . . . . . . . . . . . . . . . . . . . . . . . . .2931 4 mL Vials, Caps and Septa . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .32 Headspace Crimp Top Vials, Caps, Septa and Kits . . . . . . . . . . . . . . . . . . . . . . . . .3335 General Purpose Sample and Storage Vials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3536 Crimping and Decapping Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3738 Vial Racks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38 Syringes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39 Autosampler Syringes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4042 Manual Syringes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4346 Gas Management Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .47 Gas Purifier Selection Guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4849 Gas Purifier Capacity Chart . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .50 Moisture Traps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5155 Oxygen Traps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5657 Hydrocarbon Traps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5759 Combination Traps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6062 Gas Purification Systems, Accessories and Kits . . . . . . . . . . . . . . . . . . . . . . . . . . .6370 GC Installation Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7172 Flowmeter Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .73 Flowmeter Selection Guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .74 Flowmeters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7579 Leak Detectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .80 Regulators and Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8182 Hardware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8391 Standards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .92 GPC/GFC Standards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .92 CE and UV-Vis Standards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9394 HPLC, LC/MS and RI Standards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9495 GC Column Test Standards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9697 GC and GC/MS Instrument Standards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .98101 Integrators, Integrator Supplies and Cables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .102106 Books, Learning Software and CDs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .107109

General Chromatography

10

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General Chromatography
General Supplies

Making Your Chromatography Lab More Productive


Labs run smoothly when every detail is right. With Agilent supplies and accessories, you can be confident that they are. All of our products have been designed or selected by our instrument design teams, manufactured to our demanding specifications, and tested in a variety of conditions to ensure a PerfectFit with Agilent instruments. That painstaking care registered to ISO 9001ensures that Agilent instruments will perform at optimum levels when you use Agilent products. In the following pages, you'll find what you need to make your lab more productiveday after day. You'll find the staples, from caps and vials to manual and autosampler syringes. You'll find gas management supplies, such as purifiers, regulators, flowmeters, and leak detectors. Hardware. Standards. Plus products that directly share our product and application knowledge with youthe latest in technical publications, learning software, and CDsto give you a competitive edge.

General Chromatography

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11

Vials
Vials, Caps and Septa

Vials, Caps and Septa


All Agilent Vials use USP Type 1 Borosilicate Glass
Agilent Technologies vials are made only from Type 1 Borosilicate glass and are tightly controlled for dimensional accuracy using the Optoelectronic system. The vials come in crimp top, screw top or snap top closure and are also available in deactivated or non-deactivated versions. Before doing an injection, you should think about: Type of closure: Crimp top vials, screw top vials or snap top vials While crimp tops are the least expensive, they need a crimping tool and the caps are not reusable. Screw top vials are the perfect medium for reusability and low evaporation.

General Chromatography

Type of glass or polypropylene: Clear or amber glass, polypropylene vials If your sample is light sensitive, you may choose amber glass. For most applications, standard clear glass is sufficient. For samples that may stick to glass or chemically react with glass, use polypropylene. Deactivated/non-deactivated: For samples that interact with the highly polar silanol groups on the glass surface, a deactivation procedure is performed on the vials. Need for inserts: Flat or conical with polyspring If you only have a small amount of sample, you may choose to place a limited volume insert inside the vial or choose micro sampling vials like Micro-V or High Recovery. Polyspring at the bottom of the conical insert keeps it aligned for proper injection. Type of septa: Depends on the properties of your sample, use the septum selection guide on the following page.

TIGHTLY CONTROLLED DIMENSIONS 12 mm

40 % LARGER OPENING THAN THE STANDARD MOUTH VIAL

OPTIONAL WRITE-ON PATCH WITH GRADUATIONS

1.5 mL

32 mm

Wide Opening 2 mL Vial

FLAT BOTTOM FOR INSERT STABILITY

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Vials
General Information/Vials, Caps and Septa
Autosampler Compatibility Chart: Agilent wide opening vials are compatible with
Agilent as well as non-Agilent autosamplers.
Autosampler Agilent Technologies Beckman Dynatech Finnigan Fisons Gilson Hewlett-Packard Hitachi L.E.A.P. LDC Perkin-Elmer Spark Spectra-Physics Varian Waters Model All Models Except CE, that cannot be pressurized 501, 507 42 Vial Tray, LC 2000 A200S AS 800 231-401, 232-402, 201/202, 221/222, Aspec All Models Except CE AS-2000, AS-4000

General Chromatography

A200S Marathon, Promis 4900, Integral 4000, Autosystem GC, ISS 100/ISS 200, LC 600 42-Tray Marathon/Promis, SPH 125 SpectraSystem, 8875/8880 8100/8200, 8035/9095, 8000, Marathon, Vista 2690, Alliance Systems

Caps and Septa


Vial cap septa are a critical link to optimal analysis. Each septum is specified to complement the overall system and enhance chemical performance. As with all Agilent components, rigorous testing and product specifications are done before offering the product. Agilent vial cap septa are specially formulated and constructed to provide optimum system performance with minimal coring and superior chemical inertness. The softness or hardness (durometer) is matched to the instrument and designed to work well with syringes and samples. The size of the septum is critical to proper sealing. All Agilent septa are specifically sized for proper sealing. Our thinnest septa have a special detail built into the cap to minimize septa coring and eliminate the need to replace caps with extended usage.

Septum Selection Guide


Septum Material Compatible with Incompatible with Resealability Max. Temperature

Rubber (Natural/Butyl) PTFE/Natural or Butyl Rubber Silicone/Silicone Rubber PTFE/Silicone, PTFE/Silicone/PTFE VITON

ACN, acetone, DMF, alcohols, diethylamine, DMSO, phenols PTFE resistance until punctured, then septa or liner will have compatibility of rubber Alcohol, acetone, ether, DMF, DMSO PTFE resistance until punctured, then septa will have compatibility of silicone Chlorinated solvents, benzene, toluene, alcohols, hexane, heptane

Chlorinated solvents, aromatics, hydrocarbons, carbon disulfide

Excellent Good

< 100C < 100C < 200C < 200C < 260C

ACN, THF, benzene chloroform, Excellent pyridine, toluene, hexane, heptane Average DMF, DMSO, ACN, THF, pyridine, dioxane, methanol, acetone Good

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Vials
Identification Chart

Agilent Vial Identification Chart and Actual Fill Volumes


Wide Opening Screw Top Vials: Septum Diameter 9 mm Wide Opening Crimp Top Vials: Septum Diameter 11 mm

100 L 32 mm x 12 mm

300 L

100 L

1.5 mL

1.5 mL

1.5 mL

1.5 mL

1.0 mL

General Chromatography

51820714

51832030

51813375 Wide Opening Snap Top Vials

51823454

51820567

93010978

51834731

93010977

51843551

51855821

Inserts for 2 mL,12x32 mm Wide Opening Vials 100 L 100 L 250 L

300 L

100 L

100 L 31 mm

1.5 mL

1.5 mL

1.0 mL

51820544

51834510

51820567

51834731

93010978

93010977

51811270

51832085

51813377

Narrow Opening Screw Top Vials

Inserts for 2 mL, 12x32 mm Narrow Opening Screw Top Vials 100 L 100 L 250 L

1.5 mL

51834428 Headspace Crimp Top VialsFlat and Rounded Bottom

51832088

51832089

51832090

Vials for Agilent LC Instruments

True scale: 75 mm x 22 mm

20 mL True scale: 45 mm x 22 mm

20 mL 500 l 700 L 6 mL

10 mL

10 mL

51820837

51820838

51834474

51834475

51800806

51800805

93011377

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Vials
Screw Top Vials

Agilent Screw Top Vials, Caps, Septa and Kits


Combine the excellent autosampler handling of a crimp cap profile with the ease of a screw cap. The screw caps and precision fit septa give a secure seal with the microvolume inserts.

Highlights
2 mL, 12 x 32 mm 40% larger opening than standard narrow opening vials Unique thread design for consistently secure seal (9 x 425) Precision-formed neck for optimal robotic arm handling

Rigorous quality assurance for dimensional consistency from lot to lot Uniformly flat bottom ensures compatibility with inserts Optional ceramic write-on spot with fill marks Select from several compatible microvolume inserts

General Chromatography

Screw Top Vials


Wide Opening Screw Top Glass Vials
Versatile 2 mL, 12 x 32 mm wide opening vials designed for autosamplers with rotating or robotic arm trays.
Screw Top, 2 mL Vials Clear wide opening screw top Clear wide opening screw top, write-on spot Amber wide opening screw top, write-on spot Clear wide opening screw top Clear wide opening screw top, write-on spot Amber wide opening screw top, write-on spot Unit 100/pk 100/pk 100/pk 1000/cs* 1000/cs* 1000/cs* Part No. 5182-0714 5182-0715 5182-0716 5183-2067 5183-2068 5183-2069
*Case includes 10 packs of 100 vials

P/N 5182-0714, 5182-0715, 5182-0716

Deactivated Wide Opening Screw Top Glass Vials


These wide opening 2 mL, 12 x 32 mm vials are created using a deactivation process to eliminate sample interactions. Ready-to-use for the analysis of polar compounds such as phenols, proteins, antibodies, amines, metabolites, herbicides and pesticides.
Deactivated Screw Top, 2 mL Vials Clear deactivated wide opening screw top Clear deactivated wide opening screw top, write-on spot Unit 100/pk 100/pk Part No. 5183-2070 5183-2071 5183-2072
P/N 5183-2070, 5183-2071, 5183-2072

Amber deactivated wide opening screw top, write-on spot 100/pk

Helpful Hint: Microvolume inserts are shown on pages 2728.

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Vials
Screw Top Vials and Caps 1.5 mL Wide Opening Screw Top High Recovery Glass Vials
Wide opening, 1.5 mL vials with 30 L reservoir. For sample concentration and injection without transferring to microvolume inserts.
Screw Top High Recovery Vials Clear high recovery screw top Amber high recovery screw top, write-on spot Unit 100/pk 100/pk Part No. 5183-2030 5183-2073

P/N 5183-2030, 5183-2073

Screw Top Micro-V Vial

General Chromatography

12 x 32 mm, Type 1 Borosilicate glass Compatible with standard screw closures 1.5 mL total volume Less than 5 L dead volume Designed for center needle position during sample injection (not recommended for Vortex)
Screw Top Micro-V Vials Micro-V vial, clear, screw top
P/N 5184-3550

Unit 100/pk 100/pk

Part No. 5184-3550 5184-3554

Micro-V vial, amber, screw top

Screw Caps
Polypropylene Screw Caps with Septa for Wide Opening Screw Top Vials
These precision-fit septa provide an evaporation proof seal. (See the Septum Selection Guide to choose the septum best suited for your application.)
Screw Caps Blue, PTFE/red silicone rubber septa* Green, PTFE/red silicone rubber septa* Red, PTFE/red silicone rubber septa* Black, PTFE/red silicone rubber septa* Blue, PTFE/silicone septa Green, PTFE/silicone septa
P/N 5182-0717, 5182-0718, 5182-0719

Unit 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk

Part No. 5182-0717 5182-0718 5182-0719 5185-5838 5182-0720 5182-0721 5182-0722 5182-0723 5182-0724 5182-0725 5183-2076 5183-2077 5183-2078
*Commonly referred to as red rubber

Red, PTFE/silicone septa Blue, PTFE/silicone/PTFE septa Green, PTFE/silicone/PTFE septa Red, PTFE/silicone/PTFE septa Blue, pre-slit PTFE/silicone septa Green, pre-slit PTFE/silicone septa Red, pre-slit PTFE/silicone septa

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Vials
Screw Caps and Septa New Screw Caps with Integrated Septa
Bonded caps combine innovation with time saving convenience. This chemically inert patented process produces a true bond at the molecular level of the septa and plastic cap, eliminating excess evaporation and providing proper seal with the vial. Septa are completely secure and will not fall into the vial during injection. These caps are made with PTFE/Silicone septa for excellent chemical resistance and multiple injections.
Screw Caps with Integrated Septa Blue polypropylene cap, bonded PTFE/silicone septa Unit 100/pk Part No. 5185-5823 5185-5824

Blue polypropylene cap, bonded pre-slit PTFE/silicone septa 100/pk

P/N 5185-5823

General Chromatography

Polypropylene Screw Caps (no septa)


An economical choice. Use caps and replace septa when needed. Choose from three cap colors for separating assays, lots, standards, etc.
Screw Caps without Septa Blue open top screw caps Green open top screw caps Red open top screw caps Blue PTFE-lined solid top screw caps (for storage) Unit 100/pk 100/pk 100/pk 100/pk Part No. 5182-0728 5182-0727 5182-0726 5183-2075
P/N 5182-0728, 5182-0727, 5182-0726

Septa
Septa for Wide Opening Screw Caps
Septa for Screw Caps Ivory PTFE/red silicone rubber septa* Red PTFE/white silicone/red PTFE septa Blue pre-slit PTFE/white silicone septa Red PTFE/white silicone septa Unit 100/pk 100/pk 100/pk 100/pk Part No. 5182-0731 5182-0729 5183-2074 5182-0730
Assortment of Septa for Wide Opening Screw Caps

*Commonly referred to as red rubber

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Vials
Screw Top Vial Kits

Vial Kits
Non-Assembled, 2 mL Wide Opening Screw Top Glass Vial Convenience Packs
Screw Top Convenience Packs are an easy way to get 500 of each component using one catalog number. Packed in our six drawer reusable blue plastic cabinet, 500 screw top vials and screw caps with septa installed are kept handy and dust-free. Note: Review the Septum Selection Guide to choose the septum best suited for your application.
Screw Top Vial Kit

Screw Top Vials and Caps Kits Clear vials with write-on spot, blue screw caps, PTFE/red silicone rubber septa* Amber vials with write-on spot, green screw caps, PTFE/red silicone rubber septa* Clear vials, blue screw caps, PTFE/silicone/PTFE septa Clear vials with write-on spot, blue screw caps, PTFE/silicone/PTFE septa Amber vials with write-on spot, green screw caps, PTFE/silicone/PTFE septa Clear vials, blue screw caps, PTFE/silicone septa Clear vials with write-on spot, blue screw caps, PTFE/silicone septa Amber vials with write-on spot, green screw caps, PTFE/silicone septa Clear vials, blue screw caps, pre-slit blue PTFE silicone septa Clear vials with write-on spot, blue screw caps, pre-slit blue PTFE silicone septa Amber vials with write-on spot, green screw caps, pre-slit blue PTFE silicone septa

Unit 500/pk 500/pk 500/pk 500/pk 500/pk 500/pk 500/pk 500/pk 500/pk 500/pk 500/pk

Part No. 5182-0732 5182-0867 5182-0733 5182-0736 5182-0869 5182-0737 5182-0734 5182-0868 5182-0735 5183-2079 5183-2080 5183-2081

Clear vials, blue screw caps, PTFE/red silicone rubber septa* 500/pk

General Chromatography

*Commonly referred to as red rubber

Helpful Hint: For LC specific vials please refer to the LC supplies section.

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Vials
Screw Top Vial Kits Pre-Assembled, 2 mL Wide Opening Screw Top Glass Vial Packs
Screw Top Vial Pre-assembled Packs come ready to use with cap and septum of your choice attached to the vial. A time/labor-saving product for use on your Agilent Autosampler or any rotating tray automatic sampler. Note: Review the Septum Selection Guide to choose the septum best suited for your application.
Pre-Assembled Screw Top Kits Clear vials with write-on spot, blue screw caps, PTFE/red silicone rubber septa* Amber vials with write-on spot, green screw caps, PTFE/red silicone rubber septa* Clear vials, blue screw caps, PTFE/silicone/PTFE septa Clear vials with write-on spot, blue screw caps, PTFE/silicone/PTFE septa Amber vials with write-on spot, green screw caps, PTFE/silicone/PTFE septa Clear vials, blue screw caps, PTFE/silicone septa Clear vials with write-on spot, blue screw caps, PTFE/silicone septa Amber vials with write-on spot, green screw caps, PTFE/silicone septa Clear vials, blue screw caps, pre-slit blue PTFE silicone septa Clear vials with write-on spot, blue screw caps, pre-slit blue PTFE silicone septa Unit 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk Part No. 5182-0553 5182-0864 5182-0554 5182-0555 5182-0866 5182-0556 5182-0557 5182-0865 5182-0558 5183-2082 5183-2083
Preassembled Vial Pack

Clear vials, blue screw caps, PTFE/red silicone rubber septa* 100/pk

General Chromatography

*Commonly referred to as red rubber

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Vials
Crimp Top Vials

Agilent Crimp Top Vials, Caps and Kits


The wide opening crimp cap provides a larger target area for improved auto-sampler needle accuracy. Select from four cap colors and a variety of septa. Whatever your crimp top vial needs, Agilent has what you are looking for.

Highlights
2 mL, 12 x 32 mm Tightly controlled crown for improved crimping Precision-formed neck for improved autosampler handling 40% larger opening than standard narrow opening vials Rigorous quality assurance provides dimensional consistency from lot to lot Uniformly flat bottom ensures compatibility with inserts Optional ceramic write-on spot with fill marks

General Chromatography

Vials
Wide Opening Crimp Top Glass Vials
Wide opening 2 mL, 12 x 32 mm vials. The outer diameter of the crimp head is 11 mm. Microvolume inserts for microsampling are shown on pages 2728.
Crimp Top, 2 mL Vials Clear wide opening crimp top Clear wide opening crimp top, write-on spot Amber wide opening crimp top, write-on spot Clear wide opening crimp top Clear wide opening crimp top, write-on spot
P/N 5181-3375, 5182-0543, 5181-3376

Unit 100/pk 100/pk 100/pk 1000/cs* 1000/cs* 1000/cs*

Part No. 5181-3375 5182-0543 5181-3376 5183-4491 5183-4492 5183-4493


*Case includes 10 packs of 100 vials

Amber wide opening crimp top, write-on spot

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Vials
Crimp Top Vials Deactivated Wide Opening Crimp Top Glass Vials
The same vials as described above with the addition of a deactivation process to eliminate sample interactions. Ready-to-use for polar compound analysis such as phenols, proteins, antibodies, amines, metabolites, herbicides and pesticides.
Deactivated Crimp Top Vials Clear deactivated wide opening crimp top, 2 mL Clear deactivated wide opening crimp top, write-on spot, 2 mL Amber deactivated wide opening crimp top, write-on spot, 2 mL Unit 100/pk 100/pk 100/pk Part No. 5183-4494 5183-4495 5183-4496
P/N 5183-4494, 5183-4495, 5183-4496

General Chromatography

1.5 mL Wide Opening Crimp Top High Recovery Glass Vials


Wide opening, 1.5 mL vials with 30 L reservoir. For sample concentration and injection without transferring to microvolume inserts.
High Recovery Crimp Top Vials Clear high recovery wide opening crimp top, 1.5 mL with 30 L reservoir Clear deactivated high recovery wide opening crimp top, 1.5 mL with 30 L reservoir Unit 100/pk 100/pk Part No. 5182-3454 5183-4497
P/N 5182-3454

Crimp Top Micro-V Vial


12 x 32 mm, Type 1 Borosilicate glass Compatible with standard crimp closures 1.5 mL total volume Less than 5 L dead volume Designed for center needle position during sample injection Not recommended for Vortex or in-vial sample concentration
Micro-V Crimp Top Vials Micro-V vial, clear, crimp top Micro-V vial, amber, crimp Unit 100/pk 100/pk Part No. 5184-3551 5184-3555
P/N 5184-3551

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Vials
Crimp Top Vials New Extreme Recovery Vial
Patented design with stable base for GC autosamplers Uniform internal surface without any ridges to hold back sample It has wider neck opening for easy pipetting Increased sample volume capacity versus standard inserts Low residual volume for smaller volume injections without using the inserts Safe to use with Vortex for sample concentration
Extreme Recovery Crimp Top Vials
P/N 5185-5821

Unit 100/pk

Part No. 5185-5821

1.5 mL Extreme Recovery clear crimp top vial

General Chromatography

Wide Opening Crimp Top Polypropylene Vials


Wide opening 12 x 32 mm vials manufactured from virgin polypropylene, meeting the requirements of 21 CFR 177.1520. Polypropylene is chemically resistant and the material of choice for pH sensitive samples, sodium or heavy metals analysis. Polypropylene is translucent and able to withstand temperatures to 135C. Use with crimp or snap caps.
Polypropylene Crimp Top Vials Polypropylene wide opening crimp top, 1 mL
P/N 5182-0567, 9301-0978

Unit 100/pk

Part No. 5182-0567

Polypropylene wide opening crimp top, 300 L

1000/pk 9301-0978

Wide Opening Crimp Top Glass/Plastic Vials


Most popular 12 x 32 mm wide opening vials for economical and effective microsampling. Polypropylene body with a glass flanged insert molded to the inside. Sample comes in contact only with the 100 L high quality glass insert and the septum. Use with crimp or snap caps. Note: Microvolume inserts are shown on pages 2728.
Glass/Plastic Crimp Top Vials
P/N 9301-0977

Unit

Part No. 9301-0977

Glass lined polypropylene wide opening crimp top, 100 L 100/pk

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Vials
Crimp Caps/Vial Kits

Caps
Crimp Caps with Septa
Note: Review the Septum Selection Guide to choose the septum best suited for your application.
11 mm Crimp Caps with Septa Silver aluminum, clear PTFE/red natural rubber septa Blue aluminum, clear PTFE/red natural rubber septa Green aluminum, clear PTFE/red natural rubber septa Red aluminum, clear PTFE/red natural rubber septa Silver aluminum, PTFE/silicone/PTFE septa Silver aluminum, PTFE/silicone septa Silver aluminum, black Viton septa Silver aluminum, clear PTFE/red natural rubber septa Silver aluminum, PTFE/silicone/PTFE septa Silver aluminum, PTFE/silicone septa Silver aluminum, PTFE septa Unit 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 100/pk 1000/pk 1000/pk 1000/pk 1000/pk Part No. 5181-1210 5181-1215 5181-1216 5181-1217 5181-1211 5182-0552 5181-1212 5183-4498 5183-4499 5183-4500 5182-0871
P/N 5181-1210, 5181-1215, 5181-1216, 5181-1217

General Chromatography

Vial Kits
Non-Assembled, 2 mL Wide Opening Crimp Top Glass Vial Convenience Packs
Crimp Top Vial Convenience Packs are an easy way to get 500 of each vial component using one catalog number. Packed in our six drawer reusable blue plastic cabinet, all items are kept handy and dust-free.
Crimp Top Vials and Cap Kits Clear crimp top vials, silver aluminum crimp caps, PTFE/ red rubber septa Amber crimp top vials with write-on spot, silver aluminum crimp caps, PTFE/red rubber septa Unit 500/pk 500/pk Part No. 5181-3400 5181-8801

Crimp Vial Kit

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Vials
Snap Top Vials

Agilent Snap Top Vials, Caps and Kits


The simple, efficient way to a secure seal without crimping. It's as easy as push-on, pull-off. A consistently secure seal outperforms the loose fit of other snap caps. A variety of snap caps with septa are available on page 26. Crimp caps can also be used with wide opening snap top vials. Snap cap vials cannot be pressurized. For microsampling, see microvolume inserts for wide opening vials.

Highlights
2 mL, 12 x 32 mm Use with any 11 mm snap or crimp cap Provides a total seal around the entire circumference of the vial crown 40% larger opening than standard narrow opening vials Precision-formed neck improves autosampler handling Optional ceramic write-on spot Uniformly flat bottom ensures compatibility with inserts

General Chromatography

Vials
Wide Opening Snap Top Glass Vials
Wide opening 2 mL, 12 x 32 mm vials. Microvolume inserts for microsampling are shown on pages 2728. Use with snap or crimp caps.
Snap Top Vials Clear wide opening snap top, 2 mL
P/N 5182-0544, 5182-0546, 5182-0545

Unit 100/pk 100/pk 100/pk 1000/cs* 1000/cs* 1000/cs*

Part No. 5182-0544 5182-0546 5182-0545 5183-4504 5183-4505 5183-4506


*Case includes 10 packs of 100 vials

Clear wide opening snap top, write-on spot, 2 mL Amber wide opening snap top, write-on spot, 2 mL Clear wide opening snap top, 2 mL Clear wide opening snap top, write-on spot, 2 mL Amber wide opening snap top, write-on spot, 2 mL

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Vials
Snap Top Vials Deactivated Wide Opening Snap Top Glass Vials
The 2 mL vials undergo a deactivation process to eliminate sample interactions. Ready-to-use for the analysis of polar compounds such as phenols, proteins, antibodies, amines, metabolites, herbicides and pesticides.
Deactivated Snap Top Vials (100/Pk) Clear deactivated wide opening snap top, 2 mL Clear deactivated wide opening snap top, write-on spot, 2 mL Amber deactivated wide opening snap top, write-on spot, 2 mL Part No. 5183-4507 5183-4508 5183-4509
P/N 5183-4507, 5183-4508, 5183-4509

Wide Opening Snap Top High Recovery Glass Vials

General Chromatography

Wide opening, 1.5 mL vials with 30 L reservoir. For sample concentration and injection without transferring to microvolume inserts. Use with snap or crimp caps.
Snap Top High Recovery Glass Vial (100/Pk) Clear high recovery snap top, 1.5 mL with 30 L reservoir Part No. 5183-4510

P/N 5183-4510

Snap Top Micro-V Vial


12 x 32 mm, Type 1 Borosilicate glass Compatible with standard snap or crimp closures 1.5 mL total volume Less than 5 L dead volume Designed for center needle position during sample injection Not recommended for Vortex or in-vial sample concentration
Snap Top Micro-V Vials Micro-V vial, clear, snap top Micro-V vial, amber, snap top Unit 100/pk 100/pk Part No. 5184-3552 5184-3556
P/N 5184-3550, 5184-3551

Wide Opening Snap Top Polypropylene Vials


Wide opening 12 x 32 mm vials manufactured from virgin polypropylene, meeting the requirements of 21 CFR 177.1520. Polypropylene is chemically resistant and the material of choice for pH sensitive samples, sodium or heavy metals analysis. Polypropylene is translucent and able to withstand temperatures to 135C. Use with snap or crimp caps.
Snap Top Polypropylene Vials Polypropylene wide opening snap top, 1 mL Polypropylene wide opening snap top, 300 L Polypropylene wide opening snap top, 300 L Unit 100/pk 1000/pk 1000/pk Part No. 5182-0567 9301-0978 5183-4731
P/N 5182-0567, 9301-0978

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Vials
Snap Top Vials and Caps Wide Opening Snap Top Glass/Plastic Vials
The most popular 2 mL, 12 x 32 mm wide opening vials for economical and effective microsampling. Polypropylene body with a glass flanged insert molded to the inside. Sample comes in contact only with the 100 L high quality glass insert and the septum. Use with snap or crimp caps.
Snap Top Glass/Plastic Vials (100/Pk) Glass lined polypropylene wide opening snap top, 100 L
P/N 9301-0977

Part No. 9301-0977

Helpful Hint: For a low cost alternative, use microvolume inserts shown on page 28.

Caps
General Chromatography
Snap Caps with 11 mm Septa
Review the Septum Selection Guide to choose the septum best suited for your application.
Snap Caps, 11 mm (100/Pk) Clear polyethylene cap, solid polyethylene membrane septa Clear polyethylene cap, clear PTFE/red silicone rubber septa* Blue polyethylene cap, clear PTFE/red silicone rubber septa* Green polyethylene cap, clear PTFE/red silicone rubber septa* Red polyethylene cap, clear PTFE/red silicone rubber septa* Clear polyethylene cap, PTFE septa
P/N 5182-0542, 5182-0550, 5182-3458, 5182-3457, 5182-3459

Part No. 5182-0542 5182-0550 5182-3458 5182-3457 5182-3459 5182-0540 5182-0566 5182-0541 5183-4511 5182-0870
*Commonly referred to as red rubber

Clear polyethylene cap, PTFE/silicone/PTFE septa Blue polyethylene cap, PTFE/silicone septa Clear polyethylene cap, pre-slit PTFE/silicone septa Clear polyethylene cap, Viton septa

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Vials
Snap Top Vials and Caps/Wide Opening Inserts

Vial Kits
Non-Assembled, 2 mL Wide Opening Snap Top Glass Vial Convenience Packs
Snap top convenience packs are an easy way to get 500 of each vial component using one catalog number. Packed in our six drawer reusable blue plastic cabinet, snap top vials and snap caps with septa are kept handy and dust-free.
Snap Top Vials and Caps Kits Clear snap top vials, clear polypropylene snap caps, PTFE/red rubber septa Amber snap top vials with write-on spot, clear polypropylene snap caps, PTFE/red rubber septa Part No. 5182-0547 5182-0548
Snap Top Vial Kit

General Chromatography

Agilent Microvolume Inserts for Wide Opening Vials


To meet your microsampling needs, Agilent has a variety of microvolume inserts with capacities and designs that can transform our vials from full-capacity to limited volume with one simple step. These inserts are compatible with our wide opening screw top, crimp top, and snap top vials.

Insert Highlights
Conical Inserts with Polymer Feet Feet act as shock absorber for needle Dead-volume is virtually eliminated Inserts are self-aligning and stand straight Flat Bottom Inserts Custom-fit to vial Extremely flat bottomno push-up Conical Glass Inserts Pulled-point interior Economical alternative

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Vials
Wide Opening Inserts Conical Inserts with Polymer Feet
With the correct default setting on an autosampler, these inserts have practically no dead volume. The syringe needle can strike the bottom of the insert without being damaged. The polymer feet act as a shock absorber. This allows all of the sample to be withdrawn. These inserts are self-aligning and stand straight.
Conical Inserts with Polymer Feet (100/Pk)* 100 L glass inserts with polymer feet and mandrel interior 100 L deactivated glass inserts with polymer feet and mandrel interior 100 L polypropylene inserts with polymer feet Part No. 5181-1270 5181-8872 5182-0549
*For use with Agilent Wide Opening Vials

General Chromatography

P/N 5181-1270

Conical Glass Inserts


Conical inserts require no springs and are ready to drop into the vial for injections. The most economical choice for microvolume sampling.
Conical Glass Inserts (100/Pk)* 100 L pulled-point glass inserts Part No. 5183-2085
*For use with Agilent Wide Opening Vials

P/N 5183-2085

Flat Bottom Inserts


An economical limited-volume alternative. Manufactured with tight tolerances for a custom fit. The extremely flat bottom without any push-up protects syringe needles.
Flat Bottom Inserts (500/Pk)* 250 L glass flat bottom inserts 250 L deactivated glass flat bottom inserts 250 L polypropylene flat bottom inserts
P/N 5181-3377

Part No. 5181-3377 5183-2086 5183-2087


*For use with Agilent Wide Opening Vials

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Vials
8-425 Screw Top Vials

Agilent Standard Opening Screw Top Vials


The original, smaller opening vial with an 8-425 thread size screw cap. Available with black caps and a variety of septa to meet the needs of your application. Note: For microvolume sampling, see our Microvolume Inserts section.

Vial Highlights
8-425 thread design The original, narrow neck size Uniformly flat bottom ensures compatibility with inserts

Vials
8-425 Screw Top Glass Vials
These 2 mL, 12 x 32 mm 8-425 vials accommodate a variety of autosamplers originally designed for narrow neck vials. Use with 8-425 screw caps.
8-425 Screw Top Glass Vials (100/Pk) Clear 8-425 screw top, 2 mL Amber 8-425 screw top, 2 mL Part No. 5183-4428 5183-4429
P/N 5183-4428, 5183-4429

General Chromatography

Deactivated 8-425 Screw Top Glass Vials


The same vials as described previously with the addition of a deactivation process to eliminate sample interactions. Ready-to-use for polar compound analysis such as phenols, proteins, antibodies, amines, metabolites, herbicides and pesticides. Use with 8-425 screw caps.
Deactivated 8-425 Screw Top Glass Vials (100/Pk) Clear deactivated 8-425 screw top, 2 mL Amber deactivated 8-425 screw top, 2 mL Part No. 5183-4432 5183-4433

Helpful Hint: Microvolume inserts are shown on page 31.

P/N 5183-4432, 5183-4433

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29

Vials
Caps and Septa

Caps
8-425 Screw Caps with Septa
Open top screw caps with the 8 mm septa installed. Note: See the Septum Selection Guide to choose the septum best suited for your application.
8-425 Screw Caps with Septa (100/Pk) Black 8-425 screw caps, red PTFE silicone septa Part No. 5183-4442

8-425 Screw Caps (no septa)

General Chromatography

P/N 5183-4442, 5183-4438

Open top polypropylene screw caps without septa. Recommended instead of phenolic caps. Phenolic is a sulfur-based product that can flake and contaminate samples when syringe needle pushes through septum.
8-425 Screw Caps no Septa (100/Pk) Black 8-425 screw caps Part No. 5183-4438

Septa
Septa for 8-425 Screw Top Vials
Septa for 8-425 Screw Top Vials (100/Pk) Red PTFE silicone/red PTFE septa, 8 mm
Septa for 8-425 Screw Top Vials

Part No. 5183-4436 5183-4437

Red PTFE silicone septa, 8 mm

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Vials
Inserts for 8-425 Vials/LC Vials

Inserts for 2 mL, 12 x 32 mm 8-425 Screw Top Vials


To meet your microsampling needs, Agilent has a variety of microvolume inserts with capacities and designs that can transform our vials from full-capacity to limited volume with one simple step. The inserts featured here are compatible with 8-425 screw top vials. (Not for use with wide opening vials.)

Conical Glass Inserts with Polymer Feet


Polymer feet act as the inserts' shock absorber to allow the syringe needle to strike the bottom without being damaged. This insert is self-aligning and stands straight.
Conical Glass Inserts with Polymer Feet (100/Pk) 100 L glass inserts with polymer feet. For 8-425 vials. Part No. 5183-2088
P/N 5183-2088

General Chromatography

Conical Glass Inserts


Ready-to-use and require no springs. The most economical for microvolume sampling.
Conical Glass Inserts (100/Pk) 100 L pulled-point interior glass inserts for 8-425 vials Part No. 5183-2089
P/N 5183-2089

Flat Bottom Inserts


An economical limited-volume alternative. Manufactured with tight tolerances for a custom fit. The extremely flat bottom without any push-up protects syringe needles.
100 L Flat Bottom Inserts (100/Pk) 100 L glass flat bottom inserts for 8-425 vials Part No. 5183-2090
P/N 5183-2090

Vials for Agilent Technologies Liquid Chromatographs


Agilent Technologies offers a variety of vials for Agilent's LC instruments. Choose from small volume vials, 2 mL, and 6 mL vials. Agilent minivials are designed to allow for reproducible injections of minute quantities, making your limited sample applications more effective and more accurate. Our microvials provide limited volumes for microsampling and the 6 mL capacity vial allows for larger sample injections. For a complete selection of vials, caps and septa for LC, refer to the LC section of this catalog.

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31

Vials
4 mL Vials

Agilent 4 mL Screw Top Vials, Caps and Septa


4 mL, 15 x 45 mm screw top glass vials are a custom fit to Agilent 6850 autosampler tray (G2880A) for use as sample as well as wash vials. Vials with fill marks are also appropriate for use as wash vials and waste vials in 7673 and 7683 autosamplers. These vials can also be used in Perkin-Elmer Autosystem GC, Waters 48 position tray autosampler, Shimadzu AOC-14/1400, LC-10A and the SIL-2AS. Use the 13 x 425 caps with septa for use as sample vials and with diffusion caps for wash vials. These vials can also be used as storage vials. Vial Highlights 4 mL, 15 x 45 mm Precision formed screw threads (13 x 425) for consistently secure seal Custom fit for 7673, 7683 and 6850 autosamplers Can be used as wash, waste or storage vials
P/N 5183-4448, 5183-4450

General Chromatography

Agilent 4 mL Screw Top Vials (100/Pk) Clear screw top, 4 mL, 15 x 45 vials Amber screw top, 4 mL, 15 x 45 vials Agilent Caps and Septa for 4 mL Screw Top Vials Black 13 x 425 caps, PTFE/silicone septa Black open top screw caps Diffusion caps PTFE/natural rubber septa Red PTFE/white silicone septa White virgin PTFE septa Agilent 4 mL Vials, Caps and Septa Kits Clear screw top wash vials with screw caps (no septa) 4 mL wash vials with fill markings and caps Unit 100/pk 100/pk 12/pk 144/pk 100/pk 1000/pk Unit 144/pk 25/pk

Part No. 5183-4448 5183-4450 Part No. 5183-4464 5183-4461 07673-40180 9301-1031 5183-4460 5183-4459 Part No. 9301-0723 5182-0551

P/N 5183-4464, 07673-40180, 5183-4464

P/N 9301-0723, 5182-0551

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Vials
Headspace Vials

Agilent Headspace Crimp Top Vials


These beveled neck vials are available in both 10 mL and 20 mL capacities, flat or rounded bottom. The 20 mm crimp caps provide a consistently secure seal. You can save when you purchase a pack quantitywith vial, cap and septa packaged together in a single container.

Vial Highlights
Beveled top for maximum secure seal Two neck lengths available Choice of a pressure safety release cap at 45 psi Available in flat or rounded bottom designs
Helpful Hint: For additional Headspace supplies see the GC Instruments Parts and Supplies section in this catalog.

General Chromatography

Vials
Flat Bottom Headspace Crimp Top Glass Vials
20 mm beveled crimp top creates a much tighter seal than flat crimp surface. The slightly longer neck is custom fit for Agilent Technologies and Carlo Erba. Manufactured to exacting tolerances. Use with 20 mm crimp caps.
Flat Bottom Headspace Crimp Top Glass Vials (100/Pk) Flat bottom Headspace, 20 mL, 23 x 75 mm Flat bottom Headspace, 10 mL, 23 x 46 mm Part No. 5182-0837 5182-0838
P/N 5182-0837, 5182-0838

Rounded Bottom Headspace Crimp Top Glass Vials


20 mm beveled crimp top creates a much tighter seal than flat crimp surface. Designed for Perkin-Elmer. Also use with Tekmar and Varian. Heat annealed to 530C. Use with 20 mm crimp caps.
Rounded Bottom Headspace Vials (100/Pk) Rounded bottom Headspace, 20 mL, 23 x 75 mm Rounded bottom Headspace, 10 mL, 23 x 46 mm Part No. 5183-4474 5183-4475
P/N 5183-4474, 5183-4475

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33

Vials
Caps

Caps
20 mm Crimp Caps Only
For use with all Headspace crimp top vials. Caps with safety feature release pressure at approximately 45 psi. Note: Review the Septum Selection Guide to choose the septum best suited for your application.
20 mm Crimp Caps Without Septa (100/Pk)
P/N 9301-0721, 9301-0718

Part No. 9301-0721

Silver aluminum, one piece crimp caps, 20 mm

Silver aluminum, one piece crimp caps with safety feature, 20 mm 9301-0718

General Chromatography

20 mm Crimp Caps with Septa


Pre-assembled 20 mm crimp caps with septa. Ready-to-use with any Headspace crimp top vials.
20 mm Crimp Caps with Septa (100/Pk)
P/N 5183-4477, 5183-4479

Part No. 5183-4478 5183-4479 5183-4480

Silver aluminum, one piece crimp caps, 20 mm PTFE/silicone septa 5183-4477 Silver aluminum, one piece crimp caps with safety feature, 20 mm PTFE/silicone septa Silver aluminum, one piece crimp caps, 20 mm molded PTFE/butyl septa Silver aluminum, one piece crimp caps with safety feature, 20 mm molded PTFE/butyl septa

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Vials
Septa/Vial Kits/Storage Vials

Septa
20 mm Septa
20 mm Septa (100/Pk) Gray butyl stopper, 20 mm Gray PTFE/black butyl molded septa, 20 mm Tan PTFE/white silicone septa, 20 mm Specs -40C to 120C -40C to 125C -60C up to 180C Part No. 5183-4476 9301-0976 9301-0719
P/N 5183-4476, 9301-0719, 9301-0976

Vial Kits
General Chromatography
Supply Kit
Supply Kit (100/Pk) 20 mL flat bottom Headspace crimp top vials, silver aluminum one-piece crimp caps with safety feature, molded PTFE/Butyl Septa 20 mL flat bottom Headspace crimp top vials, silver aluminum one-piece crimp caps with safety feature, molded PTFE/Silicone Septa Specs <120C Part No. 5182-0839

>125C

5182-0840

Supply Kits

General Purpose Sample and Storage Vials


With six vial capacities available in clear or amber glass, Agilent vials are ideal for sampling or storage. Our Teflon-lined storage caps offer broad chemical resistancethe ultimate in glass closures. The polypropylene screw caps with Teflon/silicone septa are extra clean to eliminate sample contamination.

Vial Kits
Clear Glass Sample Vials with Teflon-Lined Storage Caps
Packaged as 100 pack of shrink-wrapped clear glass sample vials, with Teflon-lined solid-top storage caps in resealable plastic bagsall in our handy container to keep items dust-free.
Vials with Solid Storage Caps 4 mL, 15 x 45-vials with 13-425 caps 12 mL, 19 x 65-vials with 15-425 caps 22 mL, 23 x 85-vials with 20-400 caps 40 mL, 28 x 95-vials with 24-400 caps Unit 100/Pk 200/Pk 200/Pk 100/Pk Part No. 5183-4519 5183-4521 5183-4523 5183-4524

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35

Vials
Storage Vials Amber Glass Sample Vials with Teflon-lined Storage Caps
Packaged as 100 pack of shrink-wrapped amber glass sample vials, with Teflon-lined solidtop storage caps in resealable plastic bags all in our handy container to keep items dust-free.
Amber Vials with Solid Storage Caps 4 mL, 15 x 45-vials with 13-425 caps 12 mL, 19 x 65-vials with 15-425 caps 40 mL, 28 x 95-vials with 24-400 caps Unit 100/pk 200/pk 200/pk Part No. 5183-4526 5183-4528 5183-4530

Clear Glass Sample Vials with Caps and Septa

General Chromatography

Vial packs are shrink-wrapped in quantities of 100 or 200. Natural colored Teflon/clear silicone septa and polypropylene open top caps are each in resealable plastic bags. All items are packed in our handy container to keep items dust-free.
Clear Vials with Caps and Septa 4 mL, 15 x 45-vials with 13-425 caps 12 mL, 19 x 65-vials with 15-425 caps 22 mL, 23 x 85 vials with 20-400 caps 40 mL, 28 x 95-vials with 24-400 caps Unit 100/pk 200/pk 200/pk 100/pk Part No. 5183-4532 5183-4534 5183-4536 5183-4537

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Vials
Electronic Crimpers and Decappers

Agilent Crimping and Decapping Tools


Electronic Crimpers and Decappers
Is your hand getting tired from crimping or decapping too many vials? Should your crimp seals be more consistent vial to vial? Try the new Electronic Crimpers and Decappers from Agilent Technologies. They crimp or decap vials easily and consistently with the push of a button. These devices, with battery-powered motors, will make crimping and decapping vials a breeze and even fun. The units use standard power tool batteries and allow you to crimp and decap hundreds of vials before you have to recharge the battery. You can adjust the crimp or decap force to your specifications.
Electronic Crimpers and Decappers Electronic Crimper: 11 mm, complete with one VersaPak Gold rechargeable battery and charger. Recommended for 2 mL, 12 x 32 mm crimp top autosampler vials. Part No. 5183-4763
Electronic Decapper P/N 5184-3567, 5184-3573

Electronic Crimper P/N 5183-4763, 5184-3572

General Chromatography

Electronic Decapper: 11 mm, complete with one VersaPak Gold 5184-3567 rechargeable battery and charger. Recommended for 2 mL, 12 x 32 mm crimp top autosampler vials. Electronic Crimper: 20 mm, complete with one VersaPak Gold rechargeable battery and charger. Recommended for 20 mm headspace or serum vials. 5184-3572

Electronic Decapper: 20 mm, complete with one VersaPak Gold 5184-3573 rechargeable battery and charger. Recommended for 20 mm headspace or serum vials. Black & Decker VersaPak Gold NiMH rechargeable battery 5183-4799

Black & Decker Rechargeable Battery P/N 5183-4799

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37

Vials
Manual Crimpers and Decappers/Vial Racks

Manual Crimpers and Decappers


8 mm Crimping and Decapping Tools
Use with all 8 mm crimp top vials and crimp caps.
Manual Crimper and Decapper

Manual Crimping and Decapping Tools Manual crimper, 8 mm Manual decapper, 8 mm Manual crimper, 11 mm Manual decapper, 11 mm Manual crimper, 20 mm Manual decapper, 20 mm Crimping System and Crimp Heads Holder without crimp head, to be mounted Crimp head, 11 mm Crimp head, 20 mm Decapper head, 11 mm Decapper head, 20 mm

Part No. 8710-1643 5181-8827 8710-0979 5181-1213 9301-0720 5181-1214 Part No. 5062-2495 5001-3755 5001-3756 5001-3758 5001-3759

General Chromatography

Vial Racks
Vial Racks
P/N 9301-0722

Part No. 5061-3349 5062-2405 9301-0722

Rack for 8 mm, 100 and 300 L minivials, solid plexiglass Rack for 8 mm, 500 and 700 L minivials, solid plexiglass Rack for 12 mm, microvials, blue plastic

P/N 5061-3349

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Syringes
General Information

Syringes
With a broad selection of syringes for manual and auto injection, Agilent has what you need for accurate and effective sampling.

Typical Needle Gauge Dimensions


OD Gauge 22 23s 25 26s mm 0.71 0.635 0.5 0.47 inches 0.028 0.025 0.02 0.185 mm 0.41 0.11 0.2 0.11 ID inches 0.016 0.0045 0.008 0.0045

General Chromatography

Needle and Point Styles


Needle gauge, length, and point styles are referenced in syringe listings as gauge/length in mm/point style (e.g., 26s/42/HP).

Needle Styles
Fixed Needle Syringe: The epoxy-cemented, stainless steel needle is centered in the syringe barrel at the depth of the zero graduation mark. Do not heat above 50C. Removable Needle Syringe: Removable needle allows use of various point styles. The threaded hub connection and Teflon sealing ferrule can be tightened to compensate for wear.

Point Styles
HP Point Style: This proprietary design yields optimal performance and reliability and reduces coring by parting, not cutting, the septum. Cone Tip: On Perkin-Elmer and Shimadzu syringes.
Cone Tip HP Point

Point Style 2: A standard, general purpose point style. Needle has a 20 bevel.
Point Style 2

Point Style 3: For manual LC injectors. The needle has a 90 bevel, ideal for a volume displacement syringe. Side Hole Point Style 5: Syringe needle has a side hole for sample filling and dispensing.

Point Style 3

Side Hole/Point Style 5

Note: For more detailed information on syringe selection, ask for pub. no. 5968-5283E.

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39

Syringes
Autosampler Syringes

Agilent Autosampler Syringes


Tapered Needle, 23-26s Gauge Autosampler Syringes
Use one needle and get the benefits of two. The upper portion of the tapered needle offers the strength of a 23 gauge, while the lower portion at 26s gauge allows for use with split/splitless or on-column injections with 0.53 mm id columns. All standard plungers are stainless steel.

Volume (L)

Tapered Needle Syringes

Unit

Needle

Part No.

Fixed needle Fixed needle Removable needle 6/pk 3/pk 6/pk 3/pk 6/pk

23-26s/42/HP 23-26s/42/HP 23-26s/42/HP 23-26s/42/HP 23-26s/42/HP 23-26s/42/HP 23-26s/42/HP 23-26s/42/HP 23-26s/42/HP 23-26s/42/HP 23-26s/42/HP 23-26s/42/HP 23-26s/42/HP

5181-1273 5181-8810 5182-0835 5182-0832 5181-1267 5181-3360 5181-3321 5181-3319 5181-3354 5181-3361 5181-3365 5181-3356 5181-3358 5183-0314 5183-2042

General Chromatography

Replacement needle for 5 L syringe 10 Fixed needle Fixed needle Removable needle Replacement needle for 10 L syringe Fixed needle, Teflon-tipped plunger Fixed needle, Teflon-tipped plunger Removable needle, Teflon-tipped plunger

Replacement plunger with Teflon tip for fixed needle 10 L syringe Replacement plunger with Teflon tip for removable needle 10 L syringe 50 100 Fixed needle, Teflon-tipped plunger Fixed needle, standard plunger

Helpful Hint: Teflon-tipped plungers are gas tight

Fixed Needle

Removable Needle

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Syringes
Autosampler Syringes Straight Needle, 23s and 26s Gauge Autosampler Syringes
Volume (L) Straight Needle Syringes Unit Needle Part No.

Fixed needle, 26 gauge Fixed needle, 26 gauge Fixed needle, 23 gauge Fixed needle, 23 gauge Removable needle, 23 gauge Replacement needle for 5 L syringe 3/pk 6/pk 6/pk 6/pk 6/pk 3/pk 6/pk 6/pk

26s/42/HP 26s/42/HP 23s/42/HP 23s/42/HP 23s/42/HP 23s/42/HP 26s/42/HP 26s/42/HP 23s/42/HP 23s/42/HP 23s/42/HP 23s/42/HP 23s/42/HP 23s/42/HP 23s/42/HP 23s/42/HP 23s/42/HP 23s/42/HP

9301-0891 5183-4728 9301-0892 5182-0875 5182-0834 5182-0830 9301-0714 5183-4729

10

Fixed needle, 26 gauge Fixed needle, 26 gauge Fixed needle, 23 gauge Fixed needle, 23 gauge Fixed needle, Teflon-tipped plunger Fixed needle, Teflon-tipped plunger Replacement plunger for 10 L fixed needle syringe Removable needle, 23 gauge Removable needle, Teflon-tipped plunger Replacement needle for 10 L syringe Replacement plunger for 10 L removable needle syringe

General Cmatography

9301-0713 9301-0725 5181-8809 5183-4730 5181-8808 5181-8806 5181-8813 5181-8811 5181-3358 5183-0316 5183-0318 5183-2058

25 50 100

Fixed needle, Teflon-tipped plunger Fixed needle, Teflon-tipped plunger Fixed needle, standard plunger

Autosampler Syringes 6-pack

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41

Syringes
Autosampler Syringes Agilent 7673/7683 On-Column Syringes
Agilent Technologies 7673/7683 on-column syringes with needle diameters for columns ranging from 0.25 mm to 0.53 mm. Specifically designed for the 7673/7683 Autosampler it's another Perfect Fit.
Volume (L) 5
Removable Needle

7673/7683 On-Column Syringes Removable needle, barrel only SS needle for 0.53 mm column SS steel needle for 0.32 mm column SS steel needle for 0.25 mm column Plunger button

Unit 3/pk 3/pk 3/pk 10/pk

Part No. 5182-0836 5182-0832 5182-0831 5182-0833 5181-8866

General Chromatography

HP 7670/71/72 Syringes
This syringe has a long needle and regular plunger button for compatibility with HP 7670/71/72 autosamplers. Available with a fixed or removable needle.
Volume (L) 1 10 10 10

7670/71/72 Syringes Removable needle Fixed needle Removable needle Fixed needle, Teflon-tipped plunger Replacement plunger for 10 L syringe

Unit

Needle 23s/56/2

Part No. 5182-9622

23s/50/HP 5182-9734 23s/50/HP 5182-9626 23s/50/HP 5182-9799 5182-9800

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Syringes
Manual Syringes

Manual Syringes
Manual, Low Volume Plunger-in-Needle Syringes
Removable needle syringes with capacities from 0.5 L to 5.0 L. The plunger is in the needle and the sample is contained within the needle.
Volume (L) 0.5 1.0 1.0 5.0

Plunger In Needle Syringes Removable needle Removable needle Replacement needle/plunger for 1.0 L syringe Removable needle Replacement needle/plunger for 1.0 L syringe Removable needle Replacement needle/plunger for 5.0 L syringe

Needle 23s/70/HP 26s/70/HP 26s/70/HP 23s/70/HP 23s/70/HP 23s/70/HP 23s/70/HP

Part No. 5182-9651 5182-9605 5182-9635 5182-9627

General Chromatography

5182-9712 5182-9623 5182-9683

Manual, Regular Plunger Syringes


Volume (L) 10 Regular Plunger Syringes Fixed needle with plunger protection Fixed needle 25 50 100 250 500 Fixed needle Fixed needle Fixed needle Fixed needle Fixed needle 10/pk Unit Needle 26s/50/2 26s/50/2 25/50/2 25/50/2 25/50/2 25/50/2 25/50/2 Part No. 5182-3499 5182-3428 5182-9625 5182-9636 5182-9615 5182-9640 5182-9641
25-500 L Fixed Needle 10 L Fixed Needle

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43

Syringes
Manual Syringes Manual Syringes with Teflon-Tipped Plunger (Gas-Tight)
Available with a fixed needle, these syringes are for general purpose injections. With gastight construction, they are ideal for sampling gases and volatile materials.
Volume Gas-Tight Manual Syringes 10 L 25 L 50 L Fixed needle, Teflon-tipped plunger Replacement plunger with Teflon tip for 10 L syringe Fixed needle, Teflon-tipped plunger Fixed needle, Teflon-tipped plunger 25s/50/2 25s/50/2 25s/50/2 25s/50/2 25/50/2 23/50/2 23/50/2 23/50/2 23/50/2 Needle 26s/50/2 Part No. 5182-9606 5182-9621 5182-9629 5182-9612 5182-9610 5182-9608 5182-9609 5182-9604 5182-9661 5182-9642 5182-9671 5182-9638 5182-9643 5182-9709

100 L Fixed needle, Teflon-tipped plunger 250 L Fixed needle, Teflon-tipped plunger

General Chromatography

Fixed Needle

500 L Fixed needle, Teflon-tipped plunger 1.0 mL Fixed needle, Teflon-tipped plunger Replacement plunger with Teflon tip for 1.0 mL syringe 2.5 mL Fixed needle, Teflon-tipped plunger Replacement plunger with Teflon tip for 2.5 mL syringe 5.0 mL Fixed needle, Teflon-tipped plunger 10 mL Fixed needle, Teflon-tipped plunger Replacement plunger with Teflon tip for 10 mL syringe

SuperfleX Plunger Syringes (not available in Japan)


The super elastic alloy plunger, together with the plunger protection guide and button reinforcement, ensure precise and smooth injections. The plunger is flexible and returns to its original shape after injection.
Volume (L) 5
Superflex Syringe

Superflex Plunger Syringes Removable needle, SuperfleX syringe Fixed needle, SuperfleX syringe (6/pk) Fixed needle, SuperfleX syringe

Needle 26s/50/2 26s/50/2 26s/50/2

Part No. 5182-3498 5182-3495 5182-3496

10 10

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Syringes
Manual Syringes On-Column Syringes
Ideal for manual on-column injections. Depending on column diameter, choose either fused silica or durable stainless steel.
Vol. (L) Manual On-Column Syringes 10 Removable needle, stainless steel Replacement needle for 10 L syringe 3/pk 10 Removable needle, fused silica (syringe only) Fused Silica needle for 10 L syringe 6/pk Replacement Teflon ferrule for P/N 9301-0658

Unit

Needle 0.23 mm /10 cm 0.23 mm /10 cm

Part No. 5182-9633 5182-9645 9301-0658


On-Column Syringe

0.18 mm/11.5 cm 19091-63000 0100-1389

General Chromatography

Gas-Tight Syringes with Luer Lock Valve


Offer pressure tight sample containment up to 100 psi for liquid and gas samples.
Volume (mL) 1 2.5 5 10 25 50

Gas-Tight Syringes w/Luer Lock Valve Gas-tight syringe, fitted with push-pull luer lock valve Gas-tight syringe, fitted with push-pull luer lock valve Gas-tight syringe, fitted with push-button luer lock valve Gas-tight syringe, fitted with push-button luer lock valve Gas-tight syringe, fitted with push-button luer lock valve Gas-tight syringe, fitted with push-button luer lock valve

Part No. 5183-4549 5183-4550 5183-4551 5183-4552 5183-4553 5183-4554


Push Button Push Pull

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45

Syringes
Manual Syringes Gas-Tight Syringes with Luer Lock
With the Teflon luer lock syringe your sample comes in contact with glass and Teflon reducing the risk of contamination. Use with syringe filters and for pump priming in LC applications.
Teflon Leur Lock

Volume (mL) 1.0 2.5 5.0

Gas-Tight Syringes w/Luer Lock Teflon luer lock Replacement plunger for 1.0 mL syringe Teflon luer lock Replacement plunger for 2.5 mL syringe Teflon luer lock Teflon luer lock Replacement plunger for 10 mL syringe Teflon luer lock

Part No. 5182-9710 5182-9661 5182-9711 5182-9671 5182-9630 5182-9620 5182-9709 5182-9639

General Chromatography

10 25

Helpful Hint: Use this product with syringe filters.

Luer Lock Needles


Luer Lock Needles
Luer Lock Needle

Unit 5/pk 2/pk 2/pk

Needle 23/50/2 23/50/5 22/51/3

Part No. 5183-4606 5183-4613 5183-4614

Needle, luer lock Needle, luer lock Needle, luer lock

LC Injector Valve Syringes


Manual Injector Valve Syringes, Rheodyne, Altex, Valco Injector Syringes
Use with a variety of liquid chromatographs including the Rheodyne, Altex and Valco sampling valves. The blunt-tip point style (3) prevents tearing of the LC injector valve needle seats.
Volume (mL) 10
Fixed Needle

Manual Injection Valve Syringes Fixed needle Fixed needle Fixed needle Fixed needle Fixed needle Fixed needle Removable needle

Needle 22/51/3 22/51/3 22/51/3 22/51/3 22/51/3 22/51/3 22/50/3

Part No. 5182-9644 5182-9628 5182-9619 5182-9613 5182-9624 5182-9658 5183-4547

25 50 100 250 500 500

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Gas Management
Gas Purifiers

Gas Purifiers
Agilent Technologies brings the highest performance and largest variety of gas purifiers to gas chromatographers. We manufacture purifiers in an astonishing variety of sizes and configurations, to remove oxygen, moisture, and hydrocarbons. Our product line contains in-line gas purifiers including refillable, indicating, S-shaped, and metal body types. In line gas purifiers are made to remove specific contaminants. We also carry gas purification systems with removable cartridges. These systems provide the ability to design the right combination of filters needed for your application. Please refer to the following Gas Purifier Selection Guide to determine which gas purifiers you should use.

General Chromatography

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Gas Management
Gas Purifiers Gas Purifier Selection Guide
In Line Purifiers
Contaminant Removed Description Series Unique Features Refillable Page No.

Moisture(H20)

Economy Indicating Moisture Traps Glass Indicating Moisture Traps S-Traps Big Moisture Traps Refills for Moisture Traps

MT, MT-D, MT-S GMT, LGMT

Economical plastic Lexan body for durability Glass body for no moisture diffusion Can be reconditioned in GC oven Ultra-high capacity for moisture Makes gas purification more economical Glass construction with plastic safety shield Economical non-indicating oxygen trap Ultra-high capacity for oxygen Economical trap for hydrocarbons Ultra-high capacity for hydrocarbons Can be reconditioned in GC oven High surface area adsorbent, purged with high purity helium Makes gas purification more economical Bi-functional trap, leak-free onepiece design Bi-functional trap, leak-free onepiece design Gas-specific (for purifying He, N2, or H2), ultra-high capacity, removes H2O, O2, HCs, CO, and CO2, recommended for GC and GC/MS gas supplies Makes gas purification more economical

Yes Yes No Yes

5153 54 55 55 55

BMT MSR-1, MSR-2, SGR, GMSR, BMSR-1 IOT, LIOT OT1 BOT HT BHT HT3 ACR, BACR OT3 HMT RMS

General Chromatography

Oxygen (02)

Indicating Oxygen Traps Oxygen Traps Big Oxygen Trap

No No No Yes Yes No Yes

56 56 57 57 58 58 58 5758

Hydrocarbons (HCS)

Hydrocarbon Traps Big Hydrocarbon Trap S-Trap Capillary Grade Hydrocarbon Trap Refills for Hydrocarbon Traps

Combination Traps

Oxygen/Moisture Trap Hydrocarbon/Moisture Trap Big Universal Trap

No Yes No

6061 61 62

Refill for Hydrocarbon/Moisture Trap HCRMS

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Purification Systems
Contaminants Description Unique Features Refillable Page No.

Multiple

High Capacity Gas Purification System

1 or 3 position cartridge system with ultra-high capacity and rugged, allwelded stainless steel construction for optimal purity, available with triple combination trap 1,2,3, or 4 position cartridge system with quick change (QC) connections and unique valves to change cartridges without interrupting gas flow to the instrument 1,2,3, or 4 position modular, quickconnect cartridge system with visible indicators

No

6364

Quick Change + Point of Operation Panel

No

6567

Super-Clean Gas Filter System

No

6869

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Gas Management
Gas Purifiers Gas Purifier Selection Guide (continued)
Other
Contaminant Removed Description Series Unique Features Refillable Page No.

Organics

Split Vent Trap

RDT

Cartridge design with durable cartridge holder and replacement cartridges

No

70

Accessories
Description Series Unique Features Page No.

Mounting Clips Trap Mounting Panel

MC, UMC UMP

Designed to hold In-Line Purifiers Designed to hold In-Line Purifiers

70 70

General Chromatography

Carrier Gas Purification

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Gas Management
Oxygen and Moisture Trap Capacities
Oxygen Removal
Type of Trap OT3 series IOT series LIOT series OT3 + IOT GC-1 GC-4 RBC series RQC series BOT series Removal Capacity (mg) 600.0 30.0 100.0 630.0 396.0 40.0 436.0 436.0 3,400.0 Performance (ppb) 15 2 1 2 5 2 2 2 1

General Chromatography

Moisture Removal
Type of Trap MT120 series MT120-D series MT120-S series MT200 series MT200-D series MT200-S series MT400 series MT400-D series MT400-S series GMT series GMT-GC series GC-2 GC-2-I BMT-series Removal Capacity (g) 21.6 21.6 31.5 36.0 36.0 52.5 72.0 72.0 105.0 16.3 11.4 15.0 7.0 130.0 Performance (ppb) 20 22 40,000 18 20 39,000 14 16 39,000 6 7 9 9 5

Performance numbers are the lowest levels observed using N2 @ 1 L/min.

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Gas Management
Moisture Traps

Moisture Removal Traps


Economy Indicating Refillable Moisture Traps
Agilent Indicating Moisture Traps are designed to remove water, oil, and organics from gases employed in, but not limited to, gas chromatography. Refillable moisture traps are constructed from Lexan brand polycarbonate tubing. Lightweight, chemically resistant and of superior strength when compared to traps manufactured from acrylic. Agilent Indicating Moisture Traps have become the chromatographers choice for water adsorption from GC gases. Available in three refillable sizes and three different packings Both inlet and outlet connectors are equipped with stainless steel frits to prevent particulates from entering the gas stream Mixed packing bed makes improper installation impossible Easily refillable Tested to 150 psi (helium)
Refillable Moisture Trap MT120

General Chromatography

Molecular Sieve 13X and Indicating 4


The preferred adsorbent for GC gas drying. Blue indicating sieve turns buff at 20% relative humidity.

Molecular Sieve 13X (MT Series)


Maximum Effluent H2O Removal Concentration Fitting (in.) Capacity (g) (ppb) Part No.

Description

Size (cc)

Refillable Moisture Trap Refillable Moisture Trap Refillable Moisture Trap Refillable Moisture Trap Refillable Moisture Trap Refillable Moisture Trap Adsorbent Refill (1 pint) for MT Series Mounting Clip for 120 & 200 cc traps Universal Mounting Clip for 400 cc traps

120 120 200 200 400 400

1/8 1/4 1/8 1/4 1/8 1/4

21.6 21.6 36.0 36.0 72.0 72.0

20 20 18 18 14 14

MT120-2 MT120-4 MT200-2 MT200-4 MT400-2 MT400-4 MSR-1

MC-1

UMC-4

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Gas Management
Moisture Traps
Molecular Sieve 5 and Indicating Drierite
High moisture capacity and simultaneously removes hydrogen sulfide and oil. Indicating Drierite changes dramatically from bright blue to pink as the gas stream approaches 40% relative humidity.

Molecular Sieve 5 (MT-D Series)


Maximum Effluent H2O Removal Concentration Fitting (in.) Capacity (g) (ppb) Part No.

Description

Size (cc)

Refillable Moisture Trap

120 120 200 200 400 400

1/8 1/4 1/8 1/4 1/8 1/4

21.6 21.6 36.0 36.0 72.0 72.0

22 22 20 20 16 16

MT120-2-D MT120-4-D MT200-2-D MT200-4-D MT400-2-D MT400-4-D MSR-2

General Chromatography

Refillable Moisture Trap Refillable Moisture Trap Refillable Moisture Trap Refillable Moisture Trap Refillable Moisture Trap Adsorbent Refill (1 pint) for MT-D Series

Mounting Clip for 120 & 200 cc traps Universal Mounting Clip for 400 cc traps

MC-1 UMC-4

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Gas Management
Moisture Traps
Silica Gel, Grade 40, and Indicating Silica Gel, Grade 42
Highest moisture capacity adsorbent. Adsorbs as much as 40% of its weight in water. High affinity for hydrocarbons. Blue indicating gel turns from a deep blue to pale pink at 40% relative humidity.

Silica Gel 40 (MT-S Series)


Maximum Effluent H2O Removal Concentration Fitting (in.) Capacity (g) (ppb) Part No.

Description

Size (cc)

Refillable Moisture Trap Refillable Moisture Trap Refillable Moisture Trap Refillable Moisture Trap Refillable Moisture Trap Refillable Moisture Trap Adsorbent Refill (1 pint) for MT-S Series

Refillable Moisture Trap MT400

120 120 200 200 400 400

1/8 1/4 1/8 1/4 1/8 1/4

31.5 31.5 52.5 52.5 105.0 105.0

40 40 39 39 39 39

MT120-2-S

General Chromatography

MT120-4-S MT200-2-S MT200-4-S MT400-2-S MT400-4-S SGR

Mounting Clip for 120 & 200 cc traps Universal Mounting Clip for 400 cc traps

MC-1 UMC-4

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Gas Management
Moisture Traps Glass Indicating Moisture Traps
Ideal for GC/MS Systems, Electron Capture Detectors, and Electrolytic Conductivity Detectors
Outlet Gas has less than 1% inlet concentration Polishing Zone Active Desiccant

Molecular Sieve 13X and a band of Indicating Molecular Sieve 4 Available in 70 cc, 100 cc and 250 cc sizes Heavy wall borosilicate glass tube no moisture diffusion Refillable
Capacity and Efficiency

General Chromatography

Mass Transfer Zone (MTZ) Gas at this point has less than 5% of inlet concentration Scrubber Zone

The 100 cc unit will treat 10 standard A cylinders with up to 30 ppm water or 16.3 grams to less than 10 ppb. Molecular sieves differ from all other commercially available adsorbents, as they have an extremely high adsorption capacity for water and polar compounds even at relatively low concentrations. The color change takes place in the middle of the trap bed, giving ample warning. In addition to the inherent qualities of the sieve material, we treat the bed material under high vacuum and heat to ensure maximum scrubbing efficiency and capacity.

Glass Indicating Moisture Traps (GMT and LGMT Series)


Maximum Moisture Effluent H2O Removal Concentration Size (cc) Fitting (in.) Capacity (g) (ppb) Part No.

Description

Desiccant is at equilibrium with inlet gas adsorbat concentration

Glass Indicating Moisture Trap Glass Indicating Moisture Trap

70 70 100 100 250 250

1/8 1/4 1/8 1/4 1/8 1/4

11.4 11.4 16.3 16.3 40.09 40.09

7 7 6 6 6 6

GMT-2GC-HP GMT-4GC-HP GMT-2-HP GMT-4-HP LGMT-2-HP LGMT-4-HP GMSR

Inlet Gas

Glass Indicating Moisture Trap Glass Indicating Moisture Trap Glass Indicating Moisture Trap Glass Indicating Moisture Trap

Refillable Glass Moisture Trap

Molecular Sieve 250 Refill for GMT and LGMT Series Mounting Clip for 70 & 100 cc traps Mounting Clip for 250 cc traps

UMC-3

UMC-4

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Gas Management
Moisture Traps Moisture Removal S-Traps
Contains Molecular Sieve 5, 45/60 mesh Can be reconditioned

Moisture Removal S-Traps


Description Moisture S trap,* preconditioned. Moisture trap: metal casing, S-shaped traps for carrier gas cleanup, 1/8 in. fittings Moisture S trap, not conditioned, 1/8 in. fittings Part No. 5060-9084
Moisture S-Trap

5060-9077

*These traps can be reconditioned by heating at a minimum of 350C, with flow for 6 hours.

Helpful Hint: Purifier log books are useful for determining maintenance schedules.

General Chromatography

Big Moisture Traps


Capacity: 130 grams H2O Pressure: Up to 250 psig Efficiency: reduction of H2O to less than 5 ppb 750 cubic centimeter gas purifiers Ideal for bulk purification applications or where several instruments are plumbed from a single gas source One piece of heavy-walled aluminum tube. This one-piece design eliminates potential leaks. Equipped with sintered stainless steel frits to prevent particulate contamination Refillable

Big Moisture Traps (BMT Series)


Description Big Moisture Trap Big Moisture Trap Refill for Big Moisture Trap (enough for 2 refills) Big Mounting Clip, 2/pk Size (cc) Fitting (in.) Part No. 750 750 1/8 1/4 BMT-2 BMT-4 BMSR-1 UMC-5-2

Moisture Trap Refills


Description Adsorbent Refill (1 pint) Adsorbent Refill (1 pint) Adsorbent Refill (1 pint) Molecular Sieve Refill (250 cc) Use with MT series MT-D series MT-S series GMT & LGMT series Part No. MSR-1 MSR-2 SGR GMSR BMSR-1
Big Moisture Trap

Refills for Big Moisture Trap (enough for 2 refills) BMT series

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Gas Management
Oxygen Traps

Oxygen Removal Traps


Indicating Oxygen Traps
Not just an Indicator, but an Indicating Trap

Indicating Oxygen Trap

Reduces oxygen to less than 1 ppb Environmentally safe Available in two sizes, standard and largethree times the capacity This trap actually removes the oxygen rather than converting it to another form of contamination. Oxygen and a wide range of oxides react with the activated bed material to form a manganese oxide. This reaction results in a progressive and dramatic color change, from light green in the adsorbents active state to deep brown as oxygen saturation occurs. The Indicating Oxygen Trap is designed to be used in conjunction with any of our high capacity, non-indicating oxygen traps. When properly installed downstream from the non-indicating trap, the Indicating Oxygen Trap provides a visual indication of oxygen breakthrough BEFORE contamination reaches sensitive system components. Unlike adsorbent materials utilized in competitive products, our indicating media can be exposed to air and water in virtually any quantity without resulting in an exothermic reaction. The spent product is nontoxic, nonhazardous, nonflammable, and nonreactive. The high impact Lexan shield prevents lab catastrophes if the trap is exposed to pressure beyond its stated pressure limit. Safe for land-fill refuse.

General Chromatography

Indicating Oxygen Traps (IOT and LIOT Series)


Description Indicating O2 Trap (IOT) Indicating O2 Trap Large Indicating O2 Trap (LIOT) Large Indicating O2 Trap Mounting Clip for IOT Trap Mounting Clip for LIOT Trap Size (cc) 30 30 150 150 Fitting (in.) 1/8 1/4 1/8 1/4 Part No. IOT-2-HP IOT-4-HP LIOT-2 LIOT-4 UMC-2 UMC-3

Economy Non-Indicating Oxygen Trap (OT1 Series)


Description Oxygen Trap Oxygen Trap Mounting Clip for OT1 Trap
Economy Non-Indicating Oxygen Trap

Size (cc) 70 70

Fitting (in.) 1/8 1/4

Part No. OT1-2 OT1-4 MC-1

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Gas Management
Oxygen Traps/Hydrocarbon Traps Big Oxygen Traps
Capacity: 3 liters O2 or 3,200 mg Pressure: Up to 250 psig Efficiency: reduction of O2 to less than 1 ppb 750 cubic centimeter gas purifiers Ideal for bulk purification applications or where several instruments are plumbed from a single gas source One piece of heavy-walled aluminum tube. This one-piece design eliminates potential leaks. Equipped with sintered stainless steel frits to prevent particulate contamination

Big Oxygen Trap

Big Oxygen Traps (BOT Series)


Description Big Oxygen Trap Big Oxygen Trap Big Mounting Clip, 2/pk Size 750 cc 750 cc Fitting (in.) 1/8 1/4 Part No. BOT-2 BOT-4 UMC-5-2

General Chromatography

Hydrocarbon Removal Traps


Hydrocarbon Traps
Our Hydrocarbon Traps offer you flexibility: refill your trap and remove extremely low levels of hydrocarbons. Remove organics from carrier gases, air and hydrogen High capacity200 cc of filtering media Impregnated carbon filter media Mounting Panel on page 70 Refillable
Hydrocarbon Trap

Hydrocarbon Traps (HT Series)


Description Refillable Hydrocarbon Trap Refillable Hydrocarbon Trap Mounting Clip for HT200 Series Adsorbent Refill (1 pint) 2 Recharges for Hydrocarbon Trap Size 200 cc 200 cc Fitting (in.) 1/8 1/4 Part No. HT200-2 HT200-4 MC-1 ACR

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Gas Management
Hydrocarbon Traps Big Hydrocarbon Trap
Capacity: 80 grams of medium to heavy molecular weight hydrocarbons Pressure: Up to 250 psig Efficiency: reduction of C4 hydrocarbons to less than 15 ppb 750 cubic centimeter gas purifiers Ideal for bulk purification applications or where several instruments are plumbed from a single gas source One piece of heavy-walled aluminum tube. This one-piece design eliminates potential leaks. Equipped with sintered stainless steel frits to prevent particulate contamination Refillable

Big Hydrocarbon Trap

Big Hydrocarbon Traps

General Chromatography

Description Big Hydrocarbon Trap Big Hydrocarbon Trap Refill for Big Hydrocarbon Trap (enough for two refills) Big Mounting Clip, 2/pk

Size 750 cc 750 cc

Fitting (in.) 1/8 1/4

Part No. BHT-2 BHT-4 BACR UMC-5-2

Hydrocarbon Removal S-Trap


Filled with 40/60 mesh activated charcoal Can be reconditioned

Hydrocarbon Removal S-Trap


Description
Hydrocarbon S-Trap

Part No. 5060-9096

Hydrocarbon S Trap: S-shaped trap used for trapping organics from gases, 1/8-in. fittings

*This trap can be reconditioned by heating at a minimum of 350C, with flow for 6 hours.

Capillary Grade Hydrocarbon Traps


Extremely high surface area, coconut shell-based, activated carbon 100 cc of filtering media Purged with ultra-high purity helium Refillable

Capillary Grade Hydrocarbon Traps


Description Capillary Grade Hydrocarbon Trap Capillary Grade Hydrocarbon Trap Mounting Clip for HT3 Series Adsorbent Refill (1 pint) 3 Recharges for Capillary Grade Hydrocarbon Trap Size 100 cc 100 cc Fitting (in.) 1/8 1/4 Part No. HT3-2 HT3-4 MC-1 ACR

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Gas Management
Hydrocarbon Traps
Helpful Hint: Agilent Hydrocarbon Traps remove many types of compounds from gas streams.

Ability of Activated Carbon to Remove Substances from Gases Using Hydrocarbon Traps
Compound acetone hypochlorous acid alcohol amines inorganic acids ammonia iodine amyl acetate isopropyl acetate amyl alcohol isopropyl alcohol benzene ketones butyl acetate butyl alcohol lactic acids by-products-organic lysol calcium hypochlorite mercaptans carbon dioxide methyl acetate chlorobenzene methyl alcohol chlorine methyl bromide chlorophenol methyl chloride chlorophyl methyl ethyl ketone cresol Efficiency excellent excellent excellent very good none poor excellent excellent excellent excellent excellent excellent excellent excellent excellent excellent very good excellent excellent excellent none excellent excellent excellent excellent excellent excellent excellent excellent excellent excellent Compound dissolved oils nitrobenzenes nitrotoluene ethyl acetate ethyl alcohol organic acids ethyl chloride oxalic acid ethyl ether ozone fluoride phenol formaldehyde potassium permanganate propyl acetate glycol propyl alcohol propyl chloride hydrogen bromide hydrogen chloride sodium hypochlorite hydrogen fluoride solvents hydrogen iodide sulfuric acid hydrogen selenide hydrogen sulfide toluene trichloroethylene xylene Efficiency excellent excellent excellent excellent excellent excellent excellent excellent excellent excellent poor excellent poor excellent excellent excellent excellent excellent satisfactory poor excellent none excellent satisfactory satisfactory satisfactory satisfactory excellent excellent excellent

General Chromatography

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Gas Management
Combination Traps

Combination Traps
Oxygen/Moisture Traps
Oxygen/moisture adsorbents team up to give you two functionalities in the same trap. Unlike some oxygen/moisture traps, these traps are disposable. Consider the safety, performance, and cost advantages of the Agilent capillary grade oxygen/ moisture traps (0T3) when comparing to heated, catalytic traps. Optimized for maximum surface area and capacity Leak-free, one-piece design(tested to 2000 psi) Bed material treated with ultra-high purity helium Filter design: prevents channeling, promotes efficient scrubbing

General Chromatography

We recommend this trap for these applications:

Electron Capture Detectors Recommended by Agilent for electron capture detectors, oxygen/water removal effectively prevents detector degradation from oxygen and water contamination. The all-metal housing of the Agilent 0T3 trap virtually eliminates contamination and resultant signal noise. GC/MS
GC/MS Evaluation of the Agilent OT3 Trap - Heres the Proof:

1. Mass spectral data was acquired to determine background. 2. The trap was installed between the gas cylinder and the GC and data was acquired. Experimental Conditions A. High purity helium B. 80-100 scans of mass spectral data were acquired. Helium admitted to MS during scans 25-45. Ten scans were summed and scans 10-20 were used to correct for inherent instrument background. C. Agilent trap was installed and experiment was repeated. D. After additional use, the experiment was repeated. The data shows about a twentyfold reduction in the M/Z 18 ratio (water) and about a fortyfold reduction in the M/Z 32 ratio (oxygen).

M/Z Ratio* Without Agilent OT3 Trap With Agilent OT3 Trap

18/28 0.098 0.005

32/28 0.266 0.0118

32/40 11.08 0.247

*Contribution of inherent instrument background removed. Analysis by: California Analytical Laboratories, Inc. Sacramento, California

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Gas Management
Combination Traps
In addition to inert gases (nitrogen, helium, argon and krypton), the Agilent OT3 Trap treats streams of hydrogen, alkanes, alkenes, aliphatic hydrocarbon gases, low boiling aromatics, carbon dioxide, carbon monoxide, and argon-methane.
Why use Oxygen Traps?

Traces of oxygen in your carrier gas system can destroy a GC column, especially polar phases.
How does it work?

The Agilent OT3 Trap contains a highly active, metal-containing, scrubbing material in an inert, aluminum body. This material removes 500 mL oxygen from a gas stream to less than 15 ppb. Additionally, up to 2 grams of moisture can be removed by this trap without affecting the oxygen capacity.

General Chromatography

Oxygen/Moisture Traps (OT3 Series)


Description Agilent OT3 Trap Agilent OT3 Trap Mounting Clip Size 100 cc 100 cc Fitting (in.) 1/8 1/4 Part No. OT3-2 OT3-4 MC-1
Agilent OT3 Trap

Hydrocarbon/Moisture Traps
Our hydrocarbon/moisture traps offer you flexibility: refill your trap and remove extremely low levels of both moisture and hydrocarbons. Replace most mixed bed traps supplied by GC manufacturers Mounting panel on page 70 Refillable

Hydrocarbon/Moisture Traps (HMT Series)


Description Refillable Hydro-Moisture Trap Refillable Hydro-Moisture Trap Mounting Clip for HMT Series Adsorbant Refill (1 pint) 2 Recharges for Hydrocarbon/Moisture Trap Size 200 cc 200 cc Fitting (in.) 1/8 1/4 Part No. HMT200-2 HMT200-4 MC-1 HCRMS
Hydrocarbon Moisture Trap

Combination Traps for Chemical Ionization MS and LC/MS


Gas Type Chemical Ionization for MS* LC/MS Nitrogen Gas Purifier** Fitting (in.) 1/8 1/4 Part No. G1999-80410 G1946-80047

*Isobutane or methane applications only. **Can be used up to 15 L/min. for LC/MS applications.

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Gas Management
Combination Traps Big Universal TrapsSuperior Gas Purifiers
Big Universal Traps utilize a layered, multi-adsorbent bed packing of the most effective, highest capacity adsorbent materials available today for the removal of oxygen, moisture, hydrocarbons, carbon dioxide and carbon monoxide from helium gas streams. The volume of the various adsorbent materials in the Big Universal Trap was developed through rigorous testing and evaluation in order to assure that breakthrough of the five major contaminant groups occurs as simultaneously as possible as each material achieves complete saturation. One Big Universal Trap unit will easily purify the contents of thirteen K size cylinders of 99.997% purity helium to a cumulative level of 100 parts per billion of O2, H2O, CO2, CO and hydrocarbons at a flow rate of up to 8 liters/minute. All tube fittings are Swagelok brand stainless steel, fitted with 40 micron stainless steel frits for particulate control. Maximum pressure is 500 psi.

General Chromatography

Big Universal Traps are more economical than other purifiers. They cost only slightly more than competitive products, but offer nearly three times the capacity. Big Universal Traps are ideal for any GC or GC/MS application where helium is employed and assurance of purity is essential. Combining the contents of three individual in-line traps into a single unit reduces the number of potential leaks and the possibility of aspirating contaminants into the gas stream. A single purifier also simplifies installation and replacement.

Capacity Data
High Purity Helium99.997% Removal Capacity

Effluent Concentration
Research Grade Helium99.9999%

Impurities N2 O2 THC* CO+CO2 H2 O H2 Ar Ne

< 1.0 ppm < 0.5 ppm < 0.5 ppm < 0.1 ppm < 0.1 ppm < 0.2 ppm < 0.2 ppm < 0.1 ppm < 0.5 ppm
*Total Hydrocarbons, analysis limited to three contaminate groups

O2 THC* H2 O

< 5 ppm < 1 ppm < 5 ppm

1.07 L 20g 46g

*Total Hydrocarbons, analysis limited to three contaminate groups

Big Universal Traps have nearly three times the capacity of the competitors purifier

Big Universal Traps (RMS Series)


Gas Type Hydrogen Hydrogen
Big Universal Trap

Fitting (in.) 1/8 1/4 1/8 1/4 1/8 1/4

Part No. RMSHY-2 RMSHY-4 RMSH-2 RMSH-4 RMSN-2 RMSN-4 UMC-5-2


+Recommended for GC Mass Spec

Helium Helium

(Ar/Me)+ (Ar/Me)+

Nitrogen Nitrogen Big Mounting Clip, 2/pk

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Gas Management
Gas Purification Systems

Gas Purification Systems


High Capacity Gas Purification System
Three Cartridge System

Agilents highest capacity and most economical gas purification system. Provides low-cost gas contaminant removal for up to 18 cylinders of carrier gas. Includes a manifold with cartridge mounts and three replaceable cartridges: one moisture/hydrocarbon cartridge; one oxygen cartridge; and one oxygen indicating cartridge. Designed to minimize column damage from impurities such as oxygen and to maximize performance by reducing sulfur compounds, moisture and organics to very low levels. First cartridge removes moisture and organics. Second cartridge provides the capacity to remove at least 1000 cc of pure oxygen. Third cartridge (indicating oxygen) changes color to warn of system saturation. Consists of a permanent all-welded stainless steel manifold to minimize the potential for leaks. Suitable for bench or wall mounting.
Single-Cartridge System

Three Cartridge High Capacity Gas Purification System

General Chromatography

Contains a triple-combination cartridge that offers the same highly efficient contaminant removal properties from eight cylinders of carrier gas but without the visual indicator. Other cartridges are also available for detector gas supplies and as individual filters for specialized requirements.

Specifications: Three Cartridge High Capacity GPS


Max. Pressure: 200 psi Capacity: 1000 mL of oxygen, 18 tanks of 99.997% purity gas 15 g H20 (inlet at saturation) 0.7 g H20 (inlet moisture 10 ppm) 12 g hydrocarbon (as n-butane) Dimensions: Weight: Purity Level: 25 cm x 30 cm x 8 cm 4.8 kg 0.2 ppm oxygen; 99.8% removal of moisture and hydrocarbons

Specifications: Single Cartridge GPS


Max. Pressure: 200 psi Capacity: 450 mL of oxygen, 8 tanks of 99.997% purity gas 6.0 g H20 (inlet at saturation) 0.3 g H20 (inlet moisture 10 ppm) 5.2g hydrocarbon (as n-butane) Dimensions: Weight: Purity Level: 12 cm x 30 cm x 8 cm 1.6 kg 0.2 ppm oxygen; 99.8% removal of moisture and hydrocarbons

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Gas Management
Gas Purification Systems
High Capacity Gas Purification System
Description Three Cartridge SystemIncludes manifold for wall or bench mount and 3 cartridges (H2O/hydrocarbon, oxygen, and indicating O2) With 1/8 in. fittings With 1/4 in. fittings Replacement Cartridge KitIncludes all three cartridges for above system. Single Cartridge SystemIncludes single cartridge manifold brackets for wall or bench mount, and a triple combination cartridge (H2O/hydrocarbon, and O2 ) 5183-1907 5182-9776 5182-9780 Part No.

General Chromatography

With 1/8 in. fittings


Single Cartridge Gas Purification System

5183-4598 5183-4599 5182-9793 5182-9795

With 1/4 in. fittings Detector-Specific PurifiersFID and ECD FID Purifier (includes two hydrocarbon/moisture cartridges with separate connections for air and hydrogen lines) 1/8 in. fittings ECD Purifier (includes moisture cartridges and high capacity oxygen cartridge in series) 1/8 in. fittings Individual Cartridge Filters and Replacement Cartridges Moisture/hydrocarbon Oxygen Indicating oxygen Moisture only Hydrocarbon only Triple combination replacement cartridge for single cartridge system Single cartridge manifold, 1/8 in. fittings, to use with any of above cartridges for wall or bench mount Single cartridge manifold, 1/4 in. fittings, to use with any of above cartridges for wall or bench mount

5182-9777 5182-9778 5182-9779 5182-9792 5182-9791 5183-4600 5183-4607 5183-4608

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Gas Management
Gas Purification Systems QC+ Point of Operation Panel
The QC+ Point of Operation Panel contains purifier cartridges that can be quickly changed (thats the QC). The cartridges are removed from the panel without interruption of gas flow to the system (thats the +), drastically minimizing costly instrument downtime (and thats a real +). Filter cartridges are of all metal or glass construction, eliminating infusion and resultant signal noise associated with filters constructed from plastics. Cartridges are quickly installed via a simple knurled retaining nut. No wrenches are needed! As many as four cartridges can be replaced in a matter of seconds, and because there is low dead volume, a minimal amount of gas system purge is required after installation. Each QC+ filter head is equipped with an internal flow valve. This valve serves two functions. In the down position, gas is passed through adsorbent filter cartridges for purification (Figure 1b). In the up position, the gas is diverted through the filter head. With the valve up (Figure 1a), the filter cartridge can then be easily replaced with a fresh cartridge. But, because the gas continues to flow through the bypass valve, the need to shut down instruments is eliminated while the filter cartridges are removed. QC+ is the only GC purification system with this revolutionary flow through/bypass feature. The QC+ system has a unique D-shaped cartridge top that is self-aligning into the matching slotted filter head, assuring trouble-free installations. As many as four cartridges can be replaced in a matter of seconds without exposing downstream plumbing to atmospheric contaminants. QC+ systems are available in single-head kits or 2-, 3- and 4-head panel configurations, available exclusively with 1/8 in. Swagelok fittings. QC+ panels can be wall mounted or used freestanding. Oxygen, water, hydrocarbons and other organics are reduced to low part per billion levels.

General Chromatography

1a

Replacement Cartridges
High Capacity Oxygen

The GC-1 oxygen cartridge is an all metal assembly that contains a highly active metal catalyst, supported on an alumina silicate substrate. Oxygen removal efficiency to less than 15 ppb. P/N: GC-1; Capacity: 396 mg
High Capacity Moisture

1b Figures 1a and 1b. The plus valve (a) features a closed gas path when changing cartridges. When placed in the down position (b), the plus valve shifts gas through the cartridge.

The GC-2 moisture cartridge is an all metal assembly that contains 13X molecular sieve, 1620 mesh sphere size. Water removal efficiency to less than 9 ppb. P/N: GC-2; Capacity: 15 g

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Gas Management
Gas Purification Systems
Replacement Cartridges (continued)
Indicating Moisture

The GC-2-I indicating moisture cartridge is a glass assembly that contains a combination of 5 molecular sieve and indicating Drierite. This cartridge provides a visual indicator of adsorbent saturation. Water removal efficiency to less than 9 ppb. P/N: GC-2-I; Capacity: 7 g
Hydrocarbon

The GC-3 hydrocarbon cartridge is an all metal assembly that contains a high surface area and coconut shell-based, activated carbon medium. Efficiencies to low ppb levels for C2 and heavier compounds. Hydrocarbon removal efficiency to less than 30 ppb. P/N: GC-3; Capacity: 8 g
Indicating Oxygen

General Chromatography

The GC-4 indicating oxygen cartridge is constructed from glass. It contains a manganese oxide adsorbent that experiences a dramatic and progressive color change during adsorption. Oxygen removal efficiency to less than 2 ppb. P/N: GC-4; Capacity: 40 mg

2-, 3- and 4-Head Panels


2-Head GC Carrier Gas

QC+ System RBC-P Two-head carrier gas purification system is strongly recommended when employing columns with bonded phases or any capillary column. Consists of high capacity oxygen/moisture adsorber and indicating trap that signals breakthrough of high capacity, non-indicating cartridge. Flow at Pressure: 13 LPM at 50 psi helium
2-Head Detector Gas Supply for FID

QC+ System RBC-D-P Two-head air/hydrogen system is strongly recommended for both hydrogen and air supply to Flame Ionization Detectors (FIDs). Includes high capacity moisture and hydrocarbon cartridges. Flow at Pressure: 14 LPM at 50 psi helium
3-Head GCs with Selective Detectors and Carrier Gas

QC+ System RTC-P Three-head cartridge system with high capacity oxygen, moisture, and hydrocarbon cartridges. The perfect compliment to any system that employs nitrogen, helium, or argon/methane mixtures. Flow at Pressure: 7 LPM at 50 psi helium
3-Head Electron Capture and ELCD Detectors

QC+ System RTC-I-P Three-head cartridge panel that includes high capacity oxygen, moisture, and indicating oxygen cartridges. Flow at Pressure: 7 LPM at 50 psi helium
4-Head GC/MS and any Critical GC Application

QC+ System RQC-P Consists of high capacity oxygen, moisture, and hydrocarbon removing filters, plus an indicating oxygen cartridge to signal oxygen breakthrough of upstream unit. The ultimate in gas purification for any GC application. Flow at Pressure: 6 LPM at 50 psi helium

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Gas Purification Systems
Quick Change Plus (QC+) Point of Operation Panels
Description
1-Head

Fitting (in.) 1/8 1/8

Part No. R2D2-P R2D2-I-P

High capacity oxygen Indicating oxygen


2-Head

High capacity oxygen, ind. oxygen Moisture, hydrocarbon Indicating moisture and hydrocarbon Moisture and indicating moisture
3-Head

1/8 1/8 1/8 1/8

RBC-P RBC-D-P RBCD-P-2I/3 RBCDP-GC2/I

RBC-P

General Chromatography

Oxygen, moisture, hydrocarbon Moisture, oxygen, ind. oxygen


4-Head

1/8 1/8

RTC-P RTC-I-P

O2, indicating O2, HC, H2O

1/8

RQC-P
RTC-I-P

Replacement Cartridges
Description High capacity oxygen High capacity moisture Indicating moisture Hydrocarbon Indicating oxygen Replacement O-ring set Part No. GC-1 GC-2 GC-2-I GC-3 GC-4 R2D2-ORINGS
RQC-P

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Gas Management
Gas Purification Systems Super-Clean Gas Filter System
Super-Clean gas filter systems are designed to provide the utmost in convenience and contamination reduction. The system is tested for leak-tightness and the glass and metal construction of the cartridges eliminates diffusion of contaminants into the gas stream. During cartridge replacement, check valves close off the system to the atmosphere, further minimizing the entry of contaminants. Filter systems are available in several configurations, including 3 and 4 cartridge systems for GCs equipped with Flame Ionization Detectors (FIDs). Additionally, baseplates are available for 2, 3, or 4 filters, allowing the user to design a gas filter system for any application. Unit is ready to connect with 1/8 in. tubing. Maximum pressure is 150 psi (11 bar). Triple filter cartridge for carrier gas purification with a single cartridge. Fuel gas filter for removal of hydrocarbons and moisture from FID fuel gases. The filter's gas flow path is made from high quality glass and stainless steel. Filters can be changed easily, without tools. Base plate design allows customization for your gas filtration needs (see figure). Indicating action tells when to replace cartridges.
Gas Filter Base Plate Design

General Chromatography

Helpful Hint: Built-in check valves automatically stop gas flow to allow for fast filter changes without the introduction of ambient air into the system.
Specifications Max pressure: Capacity: 150 psi (11 bar) Triple Filter1.8 g H2O, 0.5 L O2 Fuel gas filter3.6 g H2O Moisture filter7.2 g H2O Oxygen1000 mL of O2 Dimensions: Weight: Purity level: 33 cm x 31 cm x 11 cm4 filter system 8 kg 0.1 ppm at max flow rate of 2 L/min (max pressure 10 bar)

Super-Clean Gas Purification Systems


Description Part No. FID Gas Purification System: Dedicated three position system for purifying 5182-9703 all gases used in an FID-equipped GC. Includes the Triple Filter Cartridge for carrier gas, and two fuel gas filter cartridges for both hydrogen and zero air. Carrier Gas Purification System: Single position system perfect for GC/MS, ECD and NPD detectors. Includes the triple filter cartridge. Super-Clean Gas Purification System: For your most demanding GC application, includes four position baseplate manifold with four filter cartridges: oxygen, moisture (both with indicator), and two hydrocarbon cartridges. 5182-9704 5182-0816

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Gas Management
Gas Purification Systems
Super-Clean Gas Purification Systems (continued)
Description FID Fuel Gas Purification System: Two position system dedicated to purifying the incoming hydrogen and zero grade air directly at your FIDequipped GC. Replacement Filter Cartridges Triple Filter Cartridge: A single carrier gas filter now with hydrocarbon, moisture, and oxygen trapping capability. Includes moisture and oxygen indicator so you know exactly when to replace the cartridge. Fuel Gas Filter Cartridge: Combination hydrocarbon and moisture trap with moisture indicator. Insures the highest quality hydrogen and zero air for your FID. Moisture filter cartridge with indicator Oxygen filter cartridge with indicator Hydrocarbon filter cartridge Filter cartridge bundle of 4 (oxygen, moisture, and 2 hydrocarbon) FID Filter Cartridge Bundle - includes one Triple Filter Cartridge and two Fuel Gas Filter Cartridges. Design your own gas purification system - use the following baseplates and available filter cartridges to design a customized gas solution specific to your application needs! Single position baseplate/manifold 2 position baseplate/manifold 3 position baseplate/manifold 4 position baseplate/manifold Wall mount bracket for Super-Clean GPS Super-Clean GPS O-ring kit (8/pk, 4 ea. of 2 sizes) 5182-0815 5182-9706 5182-9707 5182-9713 5182-0821 5182-3423 5182-9705 Part No. 5183-4774

5183-4771

5182-0817 5182-0818 5182-0820 5183-4770 5183-4769

General Chromatography

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Gas Management
Gas Purifier Accessories Universal/External Split Vent Trap
Split vent trap stops environmental pollution. The split vent trap was designed to protect the lab environment from the contaminants released by split injection systems, which can vent up to 500 times the amount of sample reaching the detector into the laboratory's air. A replaceable, impregnated carbon filter media traps and eliminates a broad range of contaminants. The traps are also easy to change and come with three packs of replacement cartridges each. Replace approximately every six months.
Split Vent Trap and Cartridges P/N RDT-1020

Description Universal/external split vent trap with 3 cartridges (1/8 in. Swagelok fitting) Replacement cartridges (3/pk)

Part No. RDT-1020 RDT-1023

General Chromatography

Chemical Filters/Traps for GC 5890


These filters are packed with molecular sieve 5, 60/80 mesh, and can be reconditioned by heating in the GC oven. (Recommended replacement for P/N 5060-8299)
Description Chemical filter for 5890 internal Detector trap replacement kit Replacement detector stainless steel trap Replacement O-rings Plastic fitting Split vent trap for 5890 GC (internal) 12/pk 10/pk Units Part No. 05890-61260 19231-60790 19231-80590 5180-4181 5181-3394 19251C

Trap Mounting Panel and Mounting Clips


17 in. x 24 in. Five rows for mounting traps Designed for MC-1, UMC-2, UMC-3, UMC-4, and UMC-5 mounting clips

Trap Mounting Panel


Description Trap Mounting Panel Part No. UMP-1724

Mounting Clips for Traps


Description Clip, 120-200 cc, 7/8 1 in. od Use with Trap Series MT120, MT200, OT1, OT3, MT3, HT200, HMT Part No. MC-1 UMC-2 UMC-3 UMC-4 UMC-5 UMC-5-2
*Big Traps require two mounting clips.

Clip, universal 1-1/8 1-3/8 in. od IOT


Mounting Clip P/N MC-1

Clip, universal 1-3/8 1-5/8 in. od GMT, LIOT Clip, universal 1-5/8 1-7/8 in. od MT400, LGMT Clip, universal 2 2-1/2 in. od, 1 each Clip, universal 2 2-1/2 in. od, (2/pk) Big Traps* Big Traps*

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Gas Management
GC Installation Kits

Agilent GC Installation Kits


Agilent Installation Kits for GC are designed to provide you with the essential items necessary for a successful installation while providing a substantial cost savings over individual purchases.
Description Installation Kit with Tools for Agilent 6890 GCs without Gas Purifiers, includes: Snoop leak detector Nuts and ferrules kit, 1/8 in., brass Copper tubing, 50 ft x 1/8 in. 1/8 in. Tees, brass Tubing cutter, 1/8 in. to 5/8 in. od tubing 1/8 in. brass caps Nut driver, 7 mm Screwdriver, Torx T 10 Screwdriver, Torx, T 20 Wrench, column nut, 1/4 in. x 5/16 in. Wrench, open end, 7/16 in. x 9/16 in. Wrench, open end, 7/16 in. and 3/8 in. Wrench, open end, 7/16 in. and 1/2 in. Basic Installation Kit for Agilent 6890 GCs with Carrier Gas Purifier Train (no tools), includes: Snoop Nuts and ferrules kit, 1/8 in., brass Copper tubing, 50 ft. x 1/8 in. 1/8 in. Tees, brass Tubing cutter, 1/8 in. to 5/8 in. od tubing 1/8 in. brass caps Oxygen trap, 1/8 in. glass indicating Big Oxygen Trap, 1/8 in. high capacity, 750 cc Moisture trap, 100 cc, 1/8 in. glass indicating Activated charcoal trap, S-type, 1/8 in. Split vent trap kit 6/pk 2/pk (2 packs of 2) 20/pk 6/pk 2/pk (2 packs of 2) 20/pk Unit Part No. 19199M 9300-0311 5080-8750 5180-4196 5180-4160

General Chromatography

8710-1709 5180-4121 8710-1217 5182-3466 5182-3465 8710-0510 8710-0803 8710-0972 8710-0806 19199N 9300-0311 5080-8750 5180-4196 5180-4160 8710-1709 5180-4121 IOT-2-HP BOT-2 GMT-2-HP 5060-9096 RDT-1020

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Gas Management
GC Installation Kits
Description Basic Installation Kit for Agilent 4890 GCs, includes: Nuts and ferrules kit, 1/8 in. brass Cap, 1/8 in. brass Tee, 1/8 in. brass Union
Basic installation kit for 4890 GC

Unit 20/pk 6/pk 2/pk (2 packs of 2)

Part No. 19199P 5080-8750 5180-4121 5180-4160 5180-4196 8710-1709 9300-0311 0101-0113

Copper tubing, 50 ft. x 1/8 in. Tubing cutter, 1/8 in. to 5/8 in. od tubing Snoop leak detector 8 oz. Glass Bubble Flowmeter 100 mL Syringe 10 L, FN, 23 ga. 6/pk Open end wrench, 7/16 in. and 3/8 in. Nut, driver Screwdriver, 4 in. Pozidriv shaft No. 2 pt Screwdriver, 3 in. Pozidriv shaft No. 1 pt Wrench, column nut, 1/4 in. x 5/16 in. Wrench, open end, 7/16 in. and 9/16 in. Wrench, open end, 1/2 in. and 7/16 in.

9301-0511 8710-0972 8710-1217 8710-0900 8710-0899 8710-0510 8710-0803 8710-0806

General Chromatography

Miscellaneous Items Teflon tape Copper tubing, 50 ft. x 1/8 in. Tubing cutter, 1/8 in. to 5/8 in. od tubing General purpose fittings kit GC basic installation kit, includes copper tubing, column nuts, two moisture traps, and five wrenches 1 roll 0460-1266 5180-4196 8710-1709 5180-4161 5182-3453

Fittings Kit P/N 5780-4161

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Gas Management
Flowmeters

Flowmeters
Agilent has a flowmeter for every application...
Agilent manufactures the largest selection of flowmeters for chromatography. We have developed flowmeters for measuring capillary column flows, calibrating air pumps and flow controllers, and verifying instrument gas flows. Setting and maintaining GC flow rates greatly affect the instrument accuracy and sensitivity. Choosing a flowmeter for your application depends upon measurement speed, ease of use, accuracy and flow rate range.
Measurement Speed

Agilent offers two types of volumetric flowmetersbubble (Optiflow) and acoustic displacement (ADM and FlowTracker series)and one mass flowmeter (Veri-Flow 500). Volumetric measurements rely upon the volume of gas passing through the flow meter but not the composition. Additionally, volumetric flowmeters accurately measure from a mixed or multicomponent gas stream. Mass flowmeters are calibrated to specific gas compositions and determine the flow rate for those calibrated gases. Mass measurements are independent of temperature and pressure. With volumetric flowmeters, no adjustments are needed when switching from one gas stream to a different composition stream. So an analyst can immediately change from measuring nitrogen from a GC detector to helium split rate from an injection port, to methane from an anaerobic digestion vessel. If a mass flowmeter is used, the analyst must change instrument settings and purge the flowmeter of the initial gas before accurate readings can be taken.
Ease of Use

General Chromatography

When setting gas flows with many flowmeters, the analyst often needs more than two hands to hold and start the flowmeter. With continuous flow readings, the analyst does not have to start or stop the flowmeter with each measurement. The FlowTracker, Veri-Flow 500, and ADM flowmeters read flow values continuously.
Flow Rate Range

Low flow rates? No problem (down to 0.1 mL/min). Very high flow rates? No problem (up to 5 L/min). A wide range of flow rates? No problem (0.5-1000 mL/min using a single flowmeter).
Accuracy

Agilent accurately calibrates all flowmeters to NIST-traceable standards.

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Gas Management
Flowmeters
Flowmeter Selection Guide
Digital Flowmeters: For those who prefer data acquisition and logging with completely electronic, multifunction devices. For noncorrosive gas streams.
Description Low Flow (mL/min) High Flow (mL/min) Flow through design Gases measured (non-corrosive) Volumetric1 or mass2 flowmeter RS232 capability Unique features Power supply Page No.

Accuracy

FlowTracker 2000 03 plus Leak Detector

500

2%

Yes

N2, H2, He, Air, CO2, CH4, 95% Ar/CH4 N2, H2, He, Air, CO2, CH4, 95% Ar/CH4 All

Both

RS232 output Multifunction digital volumetric flowmeter, with leak detection and pressure, temperature, and calculated mass flow measurements RS232 output Multifunction digital volumetric flowmeter, with pressure, temperature, and calculated mass flow measurements RS232 output Easy-to-use digital volumetric flowmeter, with calculated mass flow measurement and RS232 output none Economical digital volumetric flowmeter for most chromatographic flow measurements True mass flow measurement with RS232 input and output capability

6 AA batteries or 110v/220v AC

75

FlowTracker 1000

03

500

2%

Yes

Both

6 AA batteries or 110v/220v AC 9v battery or 110v/220v AC 9v battery

75

ADM 2000

0.5

1000

3%

No

Both

7677

General Chromatography

ADM 1000

0.5

1000

3%

No

All

Volumetric

7677

Veri-Flow 500

500

3%

Yes

N2, H2, He, Mass Air, 95% Ar/CH4

RS232 input and output

Rechargeable battery or 110v/220v AC

77

Bubble Flowmeters: For those who prefer classic bubblemeter technology combined with digital readout. Durable, for all gases, including mildly corrosive and humid gas streams.
Description Low Flow (mL/min) High Flow (mL/min) Flow through design Gases measured Volumetric1 or mass2 flowmeter RS232 capability Unique features Power supply Page No.

Accuracy

Optiflow Model 420 Optiflow Model 570 Optiflow Model 650

0.1 0.5

50 700

3% 3%

No No

All All

Volumetric Volumetric

none none

Low flow range for measuring flows of capillary columns Medium flow range for general chromatography applications, also available in a field portable case High flow range for demanding flow measurements, including negative (vacuum) flows, also available in a field portable case

9v battery 9v battery

7879 7879

5000

2%

No

All

Volumetric

none

9v battery

7879

Liquid Flowmeters: For those who need to verify and set HPLC column flow rates
Description Low Flow (mL/min) High Flow (mL/min) Flow through design Liquids measured Volumetric1 or mass2 flowmeter RS232 capability Unique features Power supply Page No.

Accuracy

Optiflow Model 1000

30

2%

No

HPLC mobile phases

Volumetric

none

Unique flowmeter for measuring flows of common HPLC mobile phases

110v/220v AC

7879

1 Volumetric measurements are accurate and are independent of the gas that is being measured, thus allowing measurements of mixed gases commonly found in chromatography. 2 Mass flow measurements are very accurate for specific gases and are not affected by temperature and pressure fluctuations. 3 FlowTrackers measure flow rates very accurately down to 5 mL/min; from 0 - 5 mL/min, the standard deviation increases to approximately 5%

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Gas Management
Flowmeters FlowTrackers
Agilents FlowTracker Flowmeters are the ultimate gas flowmeters for chromatography applications. These handheld flowmeters incorporate industry leading performance and features in a highly accurate and reliable package. The inherent stability of the rugged, solid state components allows us to provide the longest calibration interval on the market, all traceable to NIST standards. There are two models available:

FlowTracker 1000
Highly accurate and reliable measurement of common carrier and fuel gases used in GC including: nitrogen, air, oxygen, hydrogen, helium, and argon/methane Two year guaranteed calibration period traceable to NIST standards Measures flow (0-500 mL/min.) based on gas viscosity properties with an accuracy of +/-2%, (+/-5% from 0-5 mL/min.) Flow rate range from 0-500 mL/min (no conversion necessary from SCCM) eliminates the need for two separate meters to measure capillary and standard flows Displays mass flow, volumetric flow, temperature, and pressure readings simultaneously Screen displays for flow, linear velocity, and split ratio modes

FlowTracker 1000 P/N 5183-4779

General Chromatography

FlowTracker 2000
Combination Flowmeter/Leak Detector: the ultimate GC troubleshooting tool! All the features of the FlowTracker 1000 plus an economical Thermal Conductivity Leak Detector in a single handheld design (leak detection at 10-3 cc/s vs. 10-5 cc/s for most dedicated leak detectors, see page 80) First of its kind product to hit the market Industry-leading flowmeter performance with leak detection for less than the price of a typical leak detector Visual and audible detection signals Cost effective alternative to purchasing two separate instruments to measure GC flows and detect gross leaks in your GC and GC/MS systems For complete specifications, ordering information, and availability, please contact your local Agilent sales office, an authorized Agilent dealer, or visit our web site.

FlowTracker 2000 P/N 5183-4780

FlowTracker Flowmeters
Description FlowTracker 1000 Flowmeter FlowTracker 2000 Flowmeter and Leak Detector FlowTracker Accessory Kit, includes adapters for various tubing sizes FlowTracker Universal AC Adapter (optional, not supplied with FlowTracker units) Part No. 5183-4779 5183-4780 5183-4788 5183-4781

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Gas Management
Flowmeters The ADM Flowmeter
Want to use the simplest flowmeter for gas chromatographic analysis? ADM flowmeters allow most gas measurements with the touch of only one buttonturn it on, and the instrument provides continuous, hands-free flow readings. You can measure column, detector, and carrier gas flows without any adjustments. ADM flowmeters are ideal for measuring gas streams with a changing gas composition. For example, if you measure the gas flow from a digestion system, concentration changes in methane, carbon dioxide, and oxygen will not affect accuracy. All ADM flowmeters are battery powered and field portable. Agilent calibrates each instrument to 5-point, NIST-traceable standards to ensure the highest available accuracy. The ADM family measures flow volumetrically, so you dont have to make any adjustments when changing from one gas to another.

General Chromatography

If you need rapid, real-time measurements of a gas stream, ADM Flowmeters are perfect for you. These flowmeters are designed for basic gas chromatography laboratories.

ADM 1000 features include:


Accuracy 3% Operating temperature range0 to 45C for the instrument, -70 to 135C for the tubing. Calibrationtraceable to NIST primary standards Real time, split ratio measurement CE mark certified Measures flow rates from 0.5- to 1000 mL/min Split ratioscompare the ratio from one gas measurements to another (i.e., injection port split ratios)

ADM 1000

ADM 2000
In addition to the features of the ADM 1000, the ADM 2000 includes: Mass flow measurementsmeasure flow rate, independent of atmospheric pressure and temperature (calculated) Data output through RS-232 port 9V battery and AC power adapter (120 or 220 VAC)
ADM 2000

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Gas Management
Flowmeters
ADM Flowmeters
Description Flow Rate (mL/min) Low High Gases* Measured Accuracy (%) Power Supply RS-232 Data Output Part No.

ADM1000 ADM2000 ADM2000E Carrying Case for ADM

0.5 0.5 0.5

1000 1000 1000

All All All

3 3 3

9V Battery Battery or 120 VAC Battery or 220 VAC

None Yes Yes

220-1170 220-1171-U 220-1171-E 907-0056


*Non-corrosive Gases Only

Veri-Flow 500
Need the ultimate in versatility? The Veri-Flow 500 packs an incredible amount of features into a highly accurate flowmeter. The solid state gas sensor used in the Veri-Flow 500 measures mass flow rates of the 5 common GC laboratory gases from 5 to 500 mL/min. Above all, the Veri-Flow 500 has big features for a small, small price.
What Does it Measure?

General Chromatography

5 gasesH2, He, N2, Air, Argon/Methane In-line flow Injection port split flows Carrier gas linear velocity
What Else?

Two-way data communication through serial port Rechargeable battery pack 3% accuracy Calibrated to a NIST primary standard CE mark certified

Electronic Mass Flowmeters


Description Flow Rate (mL/min) Low High Gases* Measured Accuracy (%) Power Supply RS-232 Data Output Part No.

Veri-Flow 500 (110 V) Veri-Flow 500 (220 V)

5.0 5.0

500 500

He, H2, Ar/CH4 N2, Air He, H2, Ar/CH4 N2, Air

3 3

Rechargeable Battery or 110 VAC Rechargeable Battery or 220 VAC

Yes Yes

HVF-500 HVF-500-2

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Gas Management
Flowmeters Digital Bubble Flowmeters
Agilent Digital Bubble Flowmeters have been making flow measurement easy for years. Today, they are still widely used to measure GC flows, calibrate air sampling pumps, and verify various gas flows. The Optiflow series offers the widest range of flow rates and allows you to visually ensure gas flows. This tried and true flowmeter measures volumetrically and therefore can measure any gas composition with the push of one button and the squeeze of a bulb.

Optiflow Features:
Calibrated to NIST primary standards Wide flow range from 0.1 to 50 mL/min (Optiflow 420) up to 5 to 5000 mL/min (OptiFlow 650) 9V Long-lifetime battery Available with hard field case (Models 570 and 650 only)

General Chromatography

Optiflow 420
The Optiflow 420 is the best bubble flowmeter for measuring low gas flow ratesespecially GC columns. Flow range0.1 to 50 mL/min +/- 3% accuracy
Optiflow 420

Optiflow 570
Want a bubble flowmeter for measuring carrier, makeup, injection port, and detector gases? The Optiflow 570 handles most of your GC needs. We also manufacture Optiflow 570 in a hard metal case, which makes it portable and field ready. Flow range0.5 to 700 mL/min for the Optiflow 570 Available in hard field case (Optiflow 570 only) Split ratio measurement +/- 3% accuracy

Optiflow 570

Optiflow 650
If you calibrate air sampling equipment, verify mass flow controller settings, or monitor higher gas flow rates, these Optiflow flowmeters are simple and efficient. Flow range5.0 to 5000 mL/min for the Optiflow 650 +/- 2% accuracy Available in hard field case Measures both positive and negative (vacuum) flow sources
Optiflow 650

Optiflow 1000Liquid Flowmeter


Optiflow 1000 measures liquids! Verify and set HPLC column flow rates with the Optiflow 1000. Flow range1 to 30 mL/min +/- 2% accuracy

Optiflow 1000

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Gas Management
Flowmeters
Optiflow Flowmeters
Gas Flowmeters Description Field Case Flow Rate (mL/min) Low High Gases* Measured Accuracy (%) Power Supply RS-232 Data Output Part No.

Optiflow 420 Optiflow 570 Field-Cal 570 Optiflow 650 Field-Cal 650

No No Yes No Yes

0.1 0.5 0.5 5.0 5.0

50 700 700 5,000 5,000

All All All All All

+/- 3 +/- 3 +/- 3 +/- 2 +/- 2

9V Battery 9V Battery 9V Battery 9V Battery 9V Battery

None None None None None

HFM-420 HFM-570 HFM-570-FC HFM-650 HFM-650-FC

Liquid Flowmeters

Optiflow 1000 (110V) Optiflow 1000 (220V)

1 1

30 30

+/- 2 +/- 2

AC Adapter, 110V AC Adapter, 220V

None None

HFM-1000

General Chromatography

HFM-1000-2

*Noncorrosive and Mildly Corrosive Gases Only

Economy Glass Bubble Flowmeters


Description 10 mL Glass Bubble Flowmeter 100 mL Glass Bubble Flowmeter Part No. 0101-0030 0101-0113

Accessories for Bubblemeters


Description Replacement Bulbs for Optiflow and Glass Bubble Flowmeters Snoop, 8 oz. Part No. HFM-BULB 9300-0311
Glass Bubble Flowmeters

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Gas Management
Leak Detectors

Leak Detection
Gas Leak Detector
The Agilent Technologies Gas Leak Detector enables quick and easy detection and measurement of gas leaks. Based on a dual cell micro volume thermal conductivity system, this unit provides very high sensitivity and eliminates contamination caused by soap solution methods. This hand-held, battery-powered detector indicates leak magnitude on the display and provides a proportional audible signal. Detachable probe and flex-and-stay extension allows access to the most difficult to reach areas. Built-in calibration for 12 gases. AC adapter/battery charger included. Helpful Hint: Using a leak detector avoids system contamination from soap solution detection methods.

General Chromatography

Specifications
Response time:
Gas Leak Detector

Approximately one second Hydrogen, Helium, Neon, Xenon, R11, R12, R21, R22, Methane, Argon, CO2, Water Vapor He 1x10-5, R12 8x10-5, CH4 1x10-4, CO2 1x10-4 (mL/sec) Dual cell micro volume thermal conductivity 6.75 in. (l) x 3 in. (w) x 1 in. (d) (172 mm x 77 mm x 26 mm) 8 in. (l) x 1.85 in. (d) (204 mm x 28 mm) Rechargeable NiCad battery, charger/AC adapter

Built-in calibration: Max. sensitivity under ideal conditions: Detection principle Console size: Probe size: Power:

Gas Leak Detector Kit


Description Leak detector includes probe, extended flexible probe, range extension nozzle, probe clip and template, cable, AC power adapter/battery charger, battery, user manual, cleaning wipe, and carrying case (available in 115 V or 220 V). Gas leak detector, 115 V Gas leak detector, 220 V Part No.

5182-9646 5182-9648

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Gas Management
Regulators and Accessories

Regulators & Tubing


Gas Cylinder Regulators
To plumb your system properly, select one of Agilents economical brass regulators and the proper gas purification devices from our newly expanded product offering. These regulators and our broad spectrum of in-line filters, combination traps, and gas purification systems are an excellent combination to plumb your systems. Our new gas purification selection guide and diagrams on pages 4849 will help you determine which products can be used with the regulators below. Helpful Hint: Always depressurize a regulator before closing the adjusting knob and removing the regulator from the cylinder.

Brass Body, Dual Stainless Steel Diaphragms


Specifications
Max inlet pressure: 3000 psig Operating -40F to 165F Temperature range: Inlet-outlet port: Outlet Valve: 1/4 in., MNPT 1/4 in., MNPT 2 1/2 in. Dual scale gauges CGA-346, 350, 340, 580, 590 Internal self-reseating relief valve 5.6 lbs

General Chromatography

Materials of Construction
Body: Bonnet: Diaphragms: Seat material: Seat assemblies: Gauges: Adjusting knob: Brass Brass Stainless steel P.T.F.E. Encapsulated Brass A.B.S. plastic
Brass Body Regulator

Outlet needle valve: Brass

Delivery pressures: 1 to 125 psig Weight:

Brass Body, Dual Stainless Steel Diaphragms (1/8 in.)*


Description CGA 346, 125 psig max (8.6 bar), Air CGA 350, 125 psig max (8.6 bar), H2, Ar/Me CGA 540, 125 psig max (8.6 bar), O2 CGA 580, 125 psig max (8.6 bar), He, Ar, N2 CGA 590, 125 psig max (8.6 bar), Air Part No. 5183-4641 5183-4642 5183-4643 5183-4644 5183-4645
*For 1/4 in. tubing, purchase a 1/4 in. adapter listed below

Regulator Outlet AdaptersFemale NPT to Swagelok-style


Description 1/4 in. to 1/8 in. brass (included with brass regulators) 1/4 in. to 1/4 in. brass* Part No. 0100-0118 0100-0119
*Required for plumbing 1/4 in. tubing to regulators above

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Gas Management
Regulators and Accessories TubingPrecleaned
Description Copper tubing, 1/8 in., 50 ft. Copper tubing, 1/8 in., 12 ft. Part No. 5180-4196 5021-7107

Spectra-Link is No Ordinary Flexible Tubing!


Stainless Steel: no outgassing or permeation through polymeric materials Quick Connection: prevents air from entering gas lines during tank changeover Tested: each system has leak rates lower than 1x10-5 cc/sec
Description Part No. SL-8 SL-4

General Chromatography

Spectra-Link with 1/8 in. fittings and 36 in. SS tubing Spectra-Link with 1/4 in. fittings and 36 in. SS tubing
Spectra-Link Tubing Connecting System on a two-stage regulator

Cylinder Bracket
Description Cylinder wall bracket with strap and chain (cylinder size up to 14 in., 35 cm) Part No. 5183-1941

Cylinder Wall Bracket P/N 5183-1941

82

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Hardware
Fittings

Nuts, Ferrules, Caps, and Plugs


Nuts, Front and Back Ferrule Sets
Description 1/16 in. SS 1/8 in. Brass 1/8 in. SS 1/4 in. Brass 1/4 in. SS Unit 10/pk 20/pk 20/pk 20/pk 20/pk Part No. 5180-4149 5080-8750 5080-8751 5080-8752 5080-8753
Nut Nut, Front and Back Ferrule Sets

Nuts
Description 1/16 in. nut, SS* 1/8 in. nut, Brass 1/4 in. nut, Brass Unit 10/pk 10/pk 10/pk Part No. 5180-4102 5180-4103 5180-4105

General Chromatography

Front Ferrules
Description 1/16 in. front ferrule, SS 1/16 in. front ferrule, Valco one piece, SS 1/8 in. front ferrule, Brass 1/4 in. front ferrule, Brass Unit 10/pk 10/pk 10/pk 10/pk Part No. 5180-4108 5181-1292 5180-4109 5180-4111

Front Ferrules

Back Ferrules

Back Ferrules
Description 1/16 in. back ferrule, SS 1/8 in. back ferrule, Brass 1/4 in. back ferrule, Brass Unit 10/pk 10/pk 10/pk Part No. 5180-4114 5180-4115 5180-4117
Cap

Caps
Description 1/8 in. cap, Brass 1/4 in. cap, Brass Unit 6/pk 6/pk Part No. 5180-4121 5180-4120
Plug

Plugs
Description 1/8 in. plug, Brass 1/4 in. plug, Brass Unit 6/pk 6/pk Part No. 5180-4124 5180-4125

Blanking Nut, Adapter, and Hose Connector


Description 1/16 in. blanking nut, SS 1/8 in. x 1/8 in. adapter, Brass 1/16 in. hose connector, Brass Unit 1/ea 1/ea 10/pk Part No. 01080-83202 0100-0420 5180-4148
* SS = Stainless Steel

Blanking Nut

Adapters

Hose Connector

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83

Hardware
Fittings

Tees, Crosses, and Elbows


Tees
Description
Tee

Unit 1/ea 2/pk 1/ea 2/pk

Part No. 0100-0782 5180-4160 0100-0542 5180-4129

1/16 in. tee, SS 1/8 in. tee, Brass 1/8 in. tee, SS 1/4 in. tee, Brass

Low Dead Volume Tee


Low Dead Volume Tee

Description 1/16 in. union tee, low dead volume, SS

Unit 1/ea

Part No. 0100-0969

General Chromatography

Crosses
Description 1/8 in., Brass
Cross

Unit 1/ea 1/ea

Part No. 0100-0161 0100-0985

1/4 in., Brass

Elbows
Description 1/8 in., Brass Unit 1/ea Part No. 0100-0091

Elbow

Unions and Connectors


Unions
Description 1/16 in. union, SS 1/16 in. union, Brass Unit 1/ea 1/ea 2/pk 1/ea 2/pk 1/ea Part No. 0100-0124 0100-1316 5180-4127 0100-0126 5180-4128 0100-0128

Union

1/8 in. union, Brass 1/8 in. union, SS 1/4 in. union, Brass 1/4 in. union, SS

Zero Dead Volume Union


Zero Dead Volume Union

Description 1/16 in. union, zero dead volume, SS

Unit 1/ea

Part No. 0100-0900

Bulkhead Unions
Description 1/8 in. bulkhead union, Brass
Bulkhead Union

Unit 1/ea 1/ea

Part No. 0100-0132 0100-0133

1/8 in. bulkhead union, SS

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Hardware
Fittings
Connectors (Swagelok-style to Male NPT)
Description 1/8 in. (A) 1/4 in. (B) connector, Brass 1/4 in. (A) 1/4 in. (B) connector, Brass Unit 2/pk 2/pk Part No. 5180-4143 5180-4145
Connector (Swagelok-style to Male NPT)

Connectors (Swagelok-style to Female NPT)


Description 1/8 in. (A) 1/4 in. (B) connector, Brass 1/4 in. (A) 1/4 in. (B) connector, Brass Unit 1/ea* 1/ea* Part No. 0100-0118 0100-0119
* Use with regulators on page 81.

Adapters and Reducers


Reducing Unions
Description 1/16 x 1/8 in. reducing union, Brass 1/16 x 1/8 in. reducing union, SS 1/8 x 1/4 in. reducing union, Brass 1/8 x 1/4 in. reducing union, SS Unit 2/pk 1/ea 2/pk 1/ea Part No. 5180-4130 0100-0241 5180-4131 0100-0121

Connector (Swagelok-style to Female NPT)

General Chromatography

Reducing Union

Reducers
Description 1/8 in. (A) 1/4 in. (B) reducer, Brass 1/4 in. (A) 1/8 in. (B) reducer, Brass Unit 2/pk 2/pk Part No. 5180-4135 5180-4134
Reducer

Fittings Kit
Description Fittings Kit-includes items below Nut, 1/8 in., Brass Nut, 1/4 in., Brass Ferrules, front 1/8 in., Brass Ferrules, front 1/4 in., Brass Ferrules, back 1/8 in., Brass Ferrules, back 1/4 in., Brass Cap, 1/4 in., Brass Cap, 1/8 in., Brass Plug, 1/8 in., Brass Plug, 1/4 in., Brass Union, 1/8 in., Brass Union, 1/4 in., Brass Union, tee, 1/8 in. Brass Union, tee, 1/4 in., Brass Union, 1/4 x 1/8 in. reducing, Brass Reducer, 1/4 x 1/8 in., Brass Reducer, 1/8 x 1/4 in., Brass www.agilent.com/chem 10/pk 10/pk 10/pk 10/pk 10/pk 10/pk 6/pk 6/pk 6/pk 6/pk 2/pk 2/pk 2/pk 2/pk 2/pk 2/pk 2/pk Unit Part No. 5180-4161 5180-4103 5180-4105 5180-4109 5180-4111 5180-4115 5180-4117 5180-4120 5180-4121 5180-4124 5180-4125 5180-4127 5180-4128 5180-4160 5180-4129 5180-4131 5180-4134 5180-4135

Fittings Kit

85

Hardware
Tools

Tools
The General Laboratory Tool Kit includes the following items: Toolbox Flashlight 8 in. bastard file 6 in. adjustable wrench 12 in. adjustable wrench for use with regulator No. 1 pt, 3 in. Pozidriv shaft, fits no. 2-4 screws No. 2 pt, 4 in. Pozidriv shaft, fits no. 5-10 screws Flathead screwdriver, 2 in. steel shaft, 1/8 in. blade Flathead screwdriver, 4 in. steel shaft, 1/4 in. blade Hex key set, 1.5, 2, 2.5, 3, 4, 5, and 6 mm keys 6 in. long jaw needle nose pliers with side cutters Three open-end wrenches (1/4 in. x 5/16 in., 1/2 in. x 9/16 in., 7/16 in. x 3/8 in.) Snoop-liquid leak detector Teflon tape
Description General Laboratory Tool Kit Part No. 5180-4162

General Laboratory Tool Kit

General Chromatography

Wrenches
Description Open end, 1/4 in. and 5/16 in. Open end, 1/2 in. and 7/16 in. Open end, 7/16 in. and 9/16 in. Open end, 7/16 in. and 3/8 in. Adjustable, 12 in., for use with regulators Open end, 9/16 in. and 5/8 in. Open end, 1/2 in. and 9/16 in. Open end, 14 mm Part No. 8710-0510 8710-0806 8710-0803 8710-0972 8710-1712 8720-0010 8720-0025 8710-1924

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Hardware
Tools
Screwdrivers
Description No. 1 pt, 3 in. Pozidriv shaft, fits no. 2-4 screws No. 2 pt, 4 in. Pozidriv shaft, fits no. 5-10 screws Part No. 8710-0899 8710-0900

Pliers and Tweezers


Description Needle nose, pointed serrated jaws Tweezers, 4 3/4 in. long Truarc pliers Part No. 8710-0004 8710-0007 8710-0018

Tube Cutters and Replacement Blades for SS or Copper Tubing

General Chromatography

Description Hi-duty tube cutter (1/8 to 1 1/8 in. od tubing) Hi-duty tube cutter blades Tube cutter (1/8 to 5/8 in. od tubing) Tube cutter blades

Unit 5/pk 5/pk

Part No. 8710-1707 8710-1708 8710-1709 8710-1710

Hex Keys
Description Hex key set, 1.5, 2, 2.5, 3, 4, and 5 mm keys, 1090A (5880/90A) Hex key, 4.0 mm, 15 cm long T-handle Hex key, 3.0 mm, 12 cm long straight-handle Hex key, 2.5 mm, 15 cm long straight-handle Hex key, 1.5 mm, 10 cm long straight-handle Hex key, 9/64 in. 15 cm long T-handle Hex key, 4.0 mm, 10 cm long straight-handle Hex key, 2.5 mm, 10 cm long straight-handle Part No. 8710-0641 8710-2392 8710-2411 8710-2412 8710-2393 8710-2394 5965-0027 5965-0028

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87

Hardware
Tools

GC Column Installation Kit


The Capillary Column Installation Kit contains: Diamond-tipped pencil 20x magnifier Ferrule removal tool Septum removal tool Refillable 2 oz. bottle of Snoop
Column Installation Kit

Description Kit with diamond-tipped cutting pencil

Part No. 430-2000

General Chromatography

GC Column Rinse Kit


This kit makes solvent rinsing easy! Solvent rinsing removes many contaminants that accumulate in columns. The contaminants are removed by passing solvents through the column. Only bonded and cross-linked phase columns should be rinsed, or permanent damage to the column will result.
Bonded Phase Column Rinse Kit

Description Capillary rinse kit

Part No. 430-3000

Fused Silica Tubing Cutters


We offer two conveniently designed, pencil-shaped tools and a ceramic wafer that allow you to make clean and easy cuts in fused silica, glass and aluminum-clad capillary columns.
Description Diamond-tipped column cutting pencil
Fused Silica Tubing Cutters

Unit

Part No. 420-1000 420-2000

Sapphire column cutting pencil Ceramic wafer column cutter GC Column cutting tool with rotating diamond Replacement diamond blade (for 5183-4620) 4/pk

5181-8836 5183-4620 5183-4621

Column Cutting Tool P/N 5183-4620

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Hardware
Tools

Miscellaneous Tools
Ferrule Tools
Remove ferrules stuck inside fittings. Screw the stainless steel ferrule tool into the ferrule and pull it out. Use the smaller capillary ferrule tool for capillary column ferrules (0.32 mm id column and smaller). The megabore ferrule tool is used for megabore ferrules. The liner tool can be used to remove polyimide and tubing from inside a megabore liner. The column ferrule installation tool positions a ferrule onto a capillary column for proper installation.

Liner Tool

Description Capillary ferrule tool Megabore ferrule tool Liner tool Column ferrule installation tool

Part No. RFT-2500 RFT-5300 RFT-5100 19251-80680

General Chromatography

20x Magnifier
Examine column ends for proper cuts. Jagged, rough, or nonperpendicular cuts negatively affect chromatographic performance.

Column Ferrule Installation Tool P/N 19251-80680

MicroprobesIdeal for Removing GC Septa and Ferrules


Hardened stainless steel probes remove septum and ferrule pieces stuck in fittings. Five different styles give you all of the angles necessary for getting into those hard-to-reach places.

Mirror
Inspect injection ports, the inside of fittings, and other hard-to-reach places.
Description Mirror 20x Magnifier Five Microprobes Part No. 707-0027 430-1020 RMP-5005
Magnifier, Mirror, and Microprobes

Five Microprobes

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89

Hardware
Tools Snoop
Leak test most pressurized gas systems. We recommend that leak detecting solutions be used with care where there is any possibility of the fluid being aspirated into gas flow streams supplying the column or detector. This squeezable bottle comes with a Snooper Tube extendable up to 12 inches (30 cm). Electronic leak detectors are preferred (see pages 75 and 80). In their absence, a volatile nonreactive solvent like isopropanol may be used. Use caution to avoid aspiration of materials such as Snoop into the column.
Description Snoop, 8 ounce (236 mL) bottle Snoop, 1 gallon bottle Part No. 9300-0311 460-1002

General Chromatography

Injection Port Cleaning Kit


If changing the liner and trimming the column does not remove contamination from your injection port, you probably need to clean and flush your injection port. Three stainless steel brushes and a scraper are the ideal tools for removing sample residue and septum particles. The stainless steel brushes have diameters of 5 mm, 1/4 in. and 3/8 in.

Injection Port Cleaning Kit

FID Cleaning Kit


This easy-to-use maintenance kit improves sensitivity, reduces noise and decreases detector spiking.
Description FID cleaning kit Injection port cleaning kit
FID Cleaning Kit

Part No. 9301-0985 480-0003

12-Piece File Set


This file set scores glass, removes burrs from metal tubing, and cleans threads. This 12-piece file set covers all the laboratory filing needs. The files are 5 1/2 in. long.
Description 12-Piece file set Part No. RSF-1200

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Hardware
Tools Combination GC Tools
Description Agilent GC Buddy-multi-purpose lab tool for GC Agilent GC Buddy Junior-multi-purpose lab tool for GC Part No. 5182-9765 5183-4789

GC Buddy

Miscellaneous Tools
Description Septum tool, knurled handle Kit, ferrule tool Capillary inlet cleaning wires Swabs 5/pk 100/pk Unit Part No. 450-1000 440-1000 5180-4153 5080-5400
GC Buddy, Jr.

General Chromatography

Septum Tool with Knurled Handle

Ferrule Tool

Capillary Inlet Cleaning Wires

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91

Chemical Standards
GPC/GFC Standards

Standards
When performing chemical analysis, you can't afford to settle for anything less than the highest standards. That is why Agilent Technologies standards are the perfect solution for you. Every Agilent standard must meet the uncompromising parameters of our ISO-9001 registered quality system. Our quality system means the strictest quality control of incoming raw materials, the meticulous validation of analyte concentrations, homogeneity and stability. So, when it's uncompromising quality you're after, whether it's analytical standards, columns or instrumentation, you can purchase them all from the same reliable source Agilent Technologies.

General Chromatography

Gel Permeation Chromatography (GPC) Calibration Standards


Three different polystyrene standard kits are available for organic GPC column calibration. Each kit contains individual monodisperse polymers that cover low, medium, and high molecular weight ranges. Each standard lot is characterized using low-angle laser light scattering, membrane osmometry, and GPC. Typical dispersity values for Agilent's polystyrene standards are 1.05 to 1.07.

GPC Calibration Standards*


Description Low molecular weight (mol wt) range, 10 standards; 162-20 K (0.5 g/ea) Medium mol wt range, 10 standards; 580-3 M (0.5 g/ea) High mol wt range, 10 standards; 300 K-15 M (0.5 g/ea) Part No. 79911-60500 79911-60501 79911-60502

*The exact molecular weights of these standards vary from batch to batch, but each batch is individually certified. The nominal molecular weights of standards are as follows: Low mol wt: 162, 580, 900, 1.4 K, 2.2 K, 3.4 K, 5.1 K, 8.1 K, 12.8 K, 20 K Medium mol wt: 580, 1.5 K, 4 K, 10 K, 27 K, 66 K, 180 K, 460 K, 1.2 M, 3 M High mol wt: 300 K, 460 K, 700 K, 1.1 M, 1.7 M, 2.6 M, 4 M, 6.2 M, 9.5 M, 15 M

GFC Calibration Standards**


Description Polysaccharides: 10 0.2 g standards in mol wt range 180-788 K PEG Standards: 10 individual 0.5 g standards in mol wt range 100-23 K Part No. 1535-4546 1535-4545

**Polysaccharides 10 individual standards 0.2 g ea. mw: 180, 738, 5900, 11800, 22800, 47300, 112000, 212000, 404000, 788000, Polyethylene glycol 10 individual standards, 0.5 g ea. mw: 106, 194, 400, 600, 1000, 2100, 4120, 7100, 11.84 K, 22.8 K

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Chemical Standards
CE and UV-Vis Standards
CE Standards
Description CE OQ/PV Kit CE IQ Kit Part No. 5063-6520 5063-6514

Note: OQ/PV=Operational Qualification/Performance Verification; IQ=Installation Qualification

Checkout Samples* for UV-Vis Spectrophotometers


Description Holmium oxide glass filter Test sample for UV-Vis (Caffeine solution 10g/mL in water) Part No. 08450-60117 5063-6524
*These are checkout samples only (not certified)

General Chromatography

Certified Calibration Standards for UV-Vis Spectrophotometers


In recent years, quality requirements as outlined by ISO 9000, GLP, GMP and NAMAS have assumed increasing importance. As a consequence, the recommendations of pharmacopoeias have become more influential in the pharmaceutical industry. Formal performance verification of UV-Visible spectrophotometers is now essential, but because of the non-availability of appropriate and/or easy-to-use standards, it has been done in ways which do not fully meet regulatory requirements or it has been very time consuming. Agilent Technologies has made performance verification of the Agilent 8453 spectrophotometer much easier by making available sets of liquid standards in snap-open ampoules. The standards can be used with standard open-topped cuvettes, but to reduce the time required and to minimize errors due to cross-contamination from standard to standard, Agilent recommends the use of flow cells for the verification. A hardware kit is available which includes all the items necessary. For reasons of safety in shipment the standards are packaged in two kits.

Standards Kit-I
Standard 60.06 mg/L potassium dichromate 0.01 N sulphuric acid 0.01 N sulphuric acid 12 g/L potassium chloride in water 10 g/L sodium iodide in water 50 g/L sodium nitrite in water 0.02% v/v toluene in hexane Purpose Photometric accuracy at 235, 257, 313 and 350 nm Blank for photometric accuracy test Stray light at 200 nm Stray light at 220 nm Stray light at 340 nm Resolution (ratio of the absorbance maximum at 269 nm to the absorbance minimum at 266 nm) Blank for resolution test

Standards Kit-II
Standard 40 g/L holmium oxide in 10% v/v perchloric acid 10% v/v perchloric acid Purpose Wavelength accuracy in UV and visible ranges Blank for wavelength accuracy

Note: HPLC grade water is recommended as blank for the stray light tests.

Hexane

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93

Chemical Standards
HPLC, LC/MS and RI Standards
UV-Vis Standards Kits and Accessories (continued)
Description OQ/PV Standards Kit-I for UV-Vis OQ/PV Standards Kit-II for UV-Vis OQ/PV Hardware kit, contains 2 flow cells (10 mm), tubings and syringes, cell cleaning fluid, Multicell transport adjustment tool Hoya 056 filter, used for performance verification of 8452A Caffeine Standard, IQ Test Sample for UV-Vis, 10 g/mL in water Part No. 5063-6503 5063-6521 5063-6523 89075-23800 08450-60300 5063-6524

Note: OQ/PV=Operational Qualification/Performance Verification; IQ=Installation Qualification

General Chromatography

LC-MS Standards
Description Caffeine Standard kit for LC/MS, 0.5, 1.0, 5.0, 25.0, and 50.0 g/mL in water Part No. 8500-6917

General LC Standards
Description Caffeine Standard for HPLC, 5.0, 25.0, 125.0, 250.0, and 500.0 g/mL in water Micro-LC Caffeine Standard kit, 2.0, 4.0, 20.0, 100.0, and 200.0 g/mL in water Fluorescence Detector Calibration Sample, 1 g glycogen Part No. 8500-6762 5065-4420 5063-6597

Qualitative LC Evaluation Standards


Description RI Detector Sample: Contains 0.3% biphenyl, 0.9% o-terphenyl in methanol. One 0.5 mL ampoule Isocratic and Gradient Standards: Contains 0.15% diethylphthalate, 0.01% biphenyl, and 0.03% terphenyl in MeOH (w/w). The gradient standard includes 0.32% dioctyl phthalate as well. Two 0.5 mL ampoules of each. Isocratic Standard: As above. One 0.5 mL ampoule. RI Detector OQ/PV Test sample, 5, 10, 15, 25, and 50 mg/mL glycerin in water Part No. 79877-68701 01080-68702 01080-68704 5064-8220

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Chemical Standards
HPLC Standards HPLC Column Selectivity Evaluation Standard
This standard is a mix of three PAH materials developed by the National Institute of Standards and Technology (NIST) for the evaluation and comparison of C18 reverse-phase materials. The sample probes the surface of the alkyl chain coating of the silica and measures the degree of polymeric coating. Highly cross-linked phases retain BaP relatively longer. Columns with similar TBN/BaP ratios will elute aromatic non-polar compounds in a very similar fashion, making the array of different C18 materials easier to manage. Each kit comes complete with full NIST certification and documentation on how to use the results. How is the sample useful? TroubleshootingBy logging the performance behavior of new columns, a variable can be eliminated whenever selectivity differences arise. Method developmentUse of the probe can help clarify a C18 phase and simplify column selection in method development.
TBN TBN

PhPh PhPh BaP BaP

Polymeric aTBN/BaP<1.0

Monomeric aTBN/BaP>1.7

General Chromatography

Separation of SRM 869 on C18

Standard Reference Material (SRM) 869


Description SRM 869 contains: 0.00112% phenanthrophenanthrene, 0.00032% tetrabenzonaphthalene and 0.00021% benzo(a)pyrene in acetonitrile. Five 1.1 mL ampoules. Part No. 5181-3353

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95

Chemical Standards
GC Column Test Standards J&W GC Column Test Standards
Compare your columns performance to the test chromatogram shipped with your J&W column from Agilent. The column test standard contains components that test the column for resolution characteristics, efficiency, and inertness. The test mixes are supplied at a concentration of 250 ng/L in 2 mL vials. Match the phase and column diameter in the chart below to find the test mix for your column. If you would like a gas test mixture for a PLOT column, please call or fax our Technical Support Department.

J&W GC Column Test Standards


Column Description CAM Carbowax20M OV-351 Cyclodex-B CycloSil-B DB -1ht
TM TM

General Chromatography

Microbore (0.05 & 0.10 mm ID) Part No.

Capillary (0.18 to 0.32 mm ID) Part No. 200-0021 200-0020 200-0032 200-0025 200-0066 200-0010

Megabore (0.45 & 0.53 mm ID) Part No.

DB-1 DB-1ms DB-5 DB-5ht DB-5ms DB-5.625 DB-17ht DB-17 DB-17ms DB-23 DB-35 DB-35ms DB-200 DB-210 DB-225 DB-502.2 DB-608 DB-624 DB-1301 DB-1701 DB-2887 DB-ALC1

200-0010 200-0010

200-0310 200-0001 200-0310 200-0010 200-0185 200-0056 200-0017

200-0110 200-0110 200-0185 200-0056 200-0117 200-0123 200-0019 200-0019 200-0200 200-0102 200-0225 200-0014 200-0117 200-0113 200-0103 200-0107 200-0110 200-0091

200-0017

200-0317 200-0317 200-0023 200-0019 200-0019 200-0200 200-0002 200-0022 200-0014 200-0017 200-0113 200-0003 200-0007 200-0091

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Chemical Standards
GC Column Test Standards
J&W Column Test Standards (continued)
Column Description DB-ALC2 DB-Dioxin DB-FFAP DB-HT Sim Dis DB-MTBE DB-Petro DB-TPH DB-VRX DB-WAX DB-WAXetr DB-XLB DX-1 DX-3 DX-4 SE-30 SE-52 SE-54 200-0070 200-0210 200-0210 200-0016 200-0015 200-0370 200-0370 200-0012 200-0061 200-0061 200-0064 200-0010 200-0010 200-0010 200-0010 200-0016
TM

Microbore (0.05 & 0.10 mm ID) Part No.

Capillary (0.18 to 0.32 mm ID) Part No. 200-0092 200-0024 200-0032

Megabore (0.45 & 0.53 mm ID) Part No. 200-0092 200-0132 200-0111

General Chromatography

200-0015 200-0070 200-0070 200-0012

Agilent GC Column Test Standards


Our line of Column Test Standards validate the performance of your Agilent columns by checking such components as polarity and inertness. These standards help insure the quality of your chromatograms.

Agilent GC Column Test Standards


Description Megabore Test Sample: 530 m HP-1, HP-5 columns (0.35-1.0 mg/mL) 1-Dodecanol Acenaphthylene n-Pentadecane n-Tridecane 4-Chlorophenol Decylamine n-Tetradecane n-Undecane Ultra Test Mix Sample: 200 m, 250 m and 320 m HP-1 and HP-5 columns (0.35-1.0 mg/mL) 1-Dodecanol Decylamine n-Pentadecane n-Tridecane 4-Chlorophenol Methylcaprate n-Tetradecane n-Undecane Acenaphthylene n-Pentane FFAP Column Test Sample: Used for all Free Fatty Acid Phase columns (0.5-1.8 mg/mL) Acetophenone Heptadecane n-Octanol n-Pentane Butyric acid Hexadecane n-Pentadecane n-Tetradecane Quantity 2 x 1 mL Part No. 8500-6812

1 x 1 mL

5080-8858

1 x 1 mL

8500-6813

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97

Chemical Standards
GC and GC/MS Instrument Standards Qualitative Instrument Evaluation Standards
GC, GC/MS, and Headspace Evaluation
Description Capillary Inlet Evaluation Sample (Split Mode): Solution of 1.0% (w/w) of C9, C16, in C14 . Three 0.5 mL ampoules. FID and TCD Sample: This sample is used for the HP 5880, 5890 and 6890 with a FID or TCD. Solution of 0.033% C14, C15, and C16 normal alkanes in hexane. Three 0.5 mL ampoules. Nitrogen-Phosphorus Detector Sample: This sample is used for the HP 5880, 5890 and 6890 with a NPD. Solution of 0.65 ppm azobenzene, 1,000 ppm octadecane, and 1.00 ppm malathion in isooctane (w/v). Three 0.5 mL ampoules. Thermal Conductivity Detector Sample: Solution of 0.33% C14, C15, and C16 normal alkanes in hexane (w/w). Three 0.5 mL ampoules. Part No. 8500-4789 18710-60170 18789-60060

18711-60060 18713-60040 19305-60580

General Chromatography

Electron Capture Detector Sample: This sample is used for the HP 5880, 5890 and 6890 with an ECD. Solution of 33 pg/mL (0.033 ppm) (w/v) each of lindane and aldrin in isooctane. Three 0.5 mL ampoules. Flame Photometric Detector Sample: This sample is used for the HP 5880, 5890 and 6890 with an FPD. Solution of 20 ng/L (20 ppm) dodecanethiol and tributylphosphate in isooctane. Three 0.5 mL ampoules.

Atomic Emission Detector Sample: Sample 1: Contains (w/w) 0.07% nitrobenzene, 0.43% n-tridecane, 0.07% 8500-5067 4-fluoroanisole, 0.05% tert-butyldisulfide, 0.08% 1,2,4-trichlorobenzene, 0.05% tetraethyl orthosilicate, 0.05% n-decane (perdeuterated), 4.3% n-dodecane, 0.13% n-tetradecane, 0.07% 1-bromohexane, 4.04% n-octane in isooctane. Sample 2: Contains the same components as Sample 1 plus 0.06% triethylphosphate. Six 0.5 mL ampoules. Three of each sample. ECD Test Sample Lindane, 0.033 ppm in iso-octane, 3 ampoules PFTBA MS Sample kit (0.5 mL ampoule) OQPV Headspace Sample, contains 0.2-0.3% t-butyl disulfide, 1,2-dichlorobenzene, and nitrobenzene in ethanol Capillary Inlet Evaluation Sample, contains 1% n-nonane and 1% n-hexadecane in n-tetradecane 5183-0379 05971-60571 5182-9733 8500-4789

Headspace Sample, contains 0.2-10.0% t-butyl disulfide, 1,2-dichlorobenzene, nitrobenzene, and n-nonane in n-dodecane 8500-4328

Hint: For more GC/MS standards see page 216.

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Chemical Standards
GC and GC/MS Instrument Standards Qualitative Simulated Distillation Standards
Qualitative Boiling Point Calibration StandardsNot for Quantitative Use
Description Boiling Point Calibration No. 1: Mixture of the following normal hydrocarbons, approximate concentrations % weight/weight: 8.3% n-pentane, 4.4% n-hexane, 4.6% n-heptane, 4.7% octane, 4.8% nonane, 9.7% decane, 4.9% n-undecane, 19.9% n-dodecane, 10.2% n-tetradecane, 5.1% n-pentadecane, 10.3% n-hexadecane, 5.3% n-heptadecane,2.2% n-octadecane, 1.3% n-eicosane, 1.3% n-tetracosane, 0.9% n-octacosane, 0.9% n-dotriacontane, 0.9% n-hexatricontane, 0.9% n-tetracontane. For use with ASTM method D 2887. Six 1 mL ampoules. Part No. 5080-8716

Low Boiling Point Calibration No. 220: Mixture of the following normal hydrocarbons, approximate concentrations 5080-8768 % weight/weight: 8.8% n-pentane, 4.7% n-hexane, 4.8% n-heptane, 4.9% n-octane, 5.1% n-nonane, 10.3% n-decane, 5.2% n-undecane, 21.1% n-dodecane, 10.8% n-tetradecane, 5.4% n-pentadecane, 10.9% n-hexadecane, 5.5% n-heptadecane,2.4% n-octadecane. Six 1 mL ampoules.

General Catography

Boiling Point Calibration No. 320: Mixture of the following components, approximate concentrations % weight/weight: 6.9% n-pentane, 4.8% 2-methyl pentane, 3.6% n-hexane, 3.7% 2,4-dimethyl pentane, 3.8% n-heptane, 4.8% toluene, 3.9%n-octane, 4.8% p-xylene, 4.8% n-propyl benzene, 8.1% n-decane, 4.7% n-butyl benzene, 16.5% n-dodecane, 4.3% n-tridecane, 8.4% n-tetradecane, 4.2% n-pentadecane, 8.5% n-hexadecane, 4.3% n-heptadecane. Six 1 mL ampoules. Internal Standard Mixture 20: Mixture of the following components, approximate concentrations % weight/weight: 33.3% n-tetradecane, 13.3% n-pentadecane, 26.7% n-hexadecane, 26.7% n-heptadecane. For use with proposed method for boiling point distribution of crude petroleum. ASTM Standards, 1976, Vol 25, pp 716-722, one 25 mL bottle.

5080-8769

5080-8723

Petrochemical
Qualitative Petrochemical Standards
Description Alcohol in Gasoline: Test mixture containing approximately 1% each of ethanol, isopropanol, n-propanol, sec-butanol, isobutyl alcohol, tert-amyl alcohol, and n-butanol; 2% each of methanol and tert-butyl alcohol; and 3% MTBE in gasoline. Three sealed 0.5 mL ampoules. Natural Gas: Approximate concentrations % volume/volume: 69% methane, 6% nitrogen, 1% carbon dioxide, 0.5% oxygen, 9% ethane, 1% n-pentane, 6% propane, 3% isobutane, 3% n-butane, 1% iso-pentane and 0.5% hexanes. One pressurized aerosol-type container (370 mL at 25 psig). Transformer Gas: Approximate composition: 0.1% each of carbon dioxide, propylene, hydrogen, carbon monoxide, propane, ethane, methane, ethylene, acetylene, butene-1, and 99% nitrogen. One pressurized aerosol-type container (370 mL at 25 psig). Refinery Gas: Approximate concentrations % volume/volume: 15% hydrogen, 5% propane, 1% propylene, 10% iso-butane, 2% iso-pentane, 1% n-pentane, 15% nitrogen, 5% methane, 5% n-butane, 10% 1-butene, 5% trans-2-butene, 5% cis-2-butene, 5% carbon dioxide, 5% carbon monoxide, 1% ethylene, and 10% ethane. One pressurized aerosol-type container (370 mL at 25 psig). Reference Gas Oil No. 1 Batch 2: Multiple component mixture of hydrocarbons from petroleum fractions. Used for performance verification of the Agilent simulated distillation analyzer, Option 840. The analyzer performs analysis of petroleum fractions according to ASTM Method D 2887. Three 1 mL ampoules. Part No. 18900-60640

5080-8756

5080-8759

5080-8755

5060-9086

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99

Chemical Standards
GC and GC/MS Instrument Standards Qualitative Standards
Miscellaneous Qualitative Standards
Description Valve Checkout: Mixture of 0.5% each of methane, pentane, and hexane in helium (98.5%). For checking backflush, gas sampling, column isolation, and other valve systems. One pressurized aerosol-type container (370 mL at 25 psig). Nickel Catalyst: Mixture of 105 ppm each of methane, carbon monoxide, and carbon dioxide in helium. One pressurized aerosol-type container (370 mL at 25 psig). Nickel Catalyst Refill MIDI System Calibration Standard: Quantitative calibration standard for the Microbial ID System. Fatty acid methyl esters w/v%. 0.01% n-C9:0, 0.02% n-C10:0, 0.01% n-C11:0, 0.02% C12:0, 0.01% C13:0, 0.02% C14:0, 0.01% n-C15:0, 0.02% n-C16:0, 0.01% n-C17:0, 0.02% n-C18:0, 0.01% n-C19:0, 0.02% n-C20:0, 0.005% C10:0 2OH, 0.0025% C10:0 3OH, 0.005% C14:0 2OH, 0.0025% C14:0 3OH and 0.005% C16:0 2OH. Four 2 mL ampoules. Hint: For a complete selection of single and multi-element reference standards for ICP-MS, refer to the ICP-MS section of this catalog on pages 699-700. Part No. 5080-8765 19354-60510 5080-8761 19298-60500

General Chromatography

Gas Analyzer Accessories and Standards


Accessories
Description Regulator for Calib Mix Cylinders, 1 ea, Regulator type: Single stage, with swivel connector, Fittings: 1/4 in. female NPT swivel to cylinder, 1/16 in. SS compression fitting to GC, Pressure gauge range: 0 to 400 psi, Flow range: Dedicated 20 mL/min 5% Carry Case for Calib Mix Cylinders, 1 ea, Dimensions: 13.5 in. x 10 in. x 4 3/8 in., Material of construction: Outer shell: High density polyethylene, Inner foam: Polyester Urethane, Holds: 2 cylinders, 2 regulators, and 2 SS pieces of gas sampling tubing Gas Sampling Tubing 1/16 in. SS with Fttgs, Specifications: 1/16 in. SS tubing, 610 mm long, 0.038 in. id with two 1/16 in. SS Swagelok nuts, front ferrules, and back ferrules Part No. 5184-3539

5184-3540

5185-5817

Gas Standard Cylinders*


Description Universal Calib Mix Cylinders, Box of 2, 0.10% Helium, 0.05% Neon, 0.10% Hydrogen, 0.05% Oxygen, 0.10% Nitrogen, Balance 99.05% Methane, 0.05% Ethane, 0.05% Ethylene, 0.05% Carbon Dioxide, 0.10% Carbon Monoxide, 0.05% Acetylene, 0.05% Propane, 0.05% Methyl Acetylene, 0.05% n-Butane, 0.05% n-Hexane, 0.05% n-Heptane NGA (Natural Gas Analyzer) Calib Mix Cylinders, Box of 2, 5.17% Nitrogen, Balance 72.24% Methane, 8.997% Ethane, 1.495% Carbon Dioxide, 6.001% Propane, 2.999% Isobutane, 2.000% n-Butane, 0.50% Isopentane, 0.50% n-Pentane, 0.10% n-Hexane RGA (Refinery Gas Analyzer) Calib Mix Cylinders, Box of 2 Part No. 5184-3541

5184-3542

5184-3543

*Cylinder specifications: Pressure (when full): 240 psi, Volume: 8.5 liters, Temperature range: 0F to 450F, Diameter: 2.75 in., Height (w/valve): 7.25 in., Tank material: Carbon steel, Concentrations: 5% accuracy, w/w.

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Chemical Standards
GC and GC/MS Instrument Standards
Gas Standard Cylinders (continued)
Description Univ/NGA Calib Mix Cylinder Combo Box** Univ/RGA Calib Mix Cylinder Combo Box** Part No. 5184-3544 5184-3545

Gas Standard Kits***


Description Universal Calibration Kit, 1 ea, with two Universal mix cylinders NGA Calibration Kit, 1 ea, with one Universal and one NGA mix cylinder RGA Calibration Kit, 1 ea, with one Universal and one RGA mix cylinder Part No. 5184-3546 5184-3547 5184-3548

**Combo boxes contain: One box of 2 calibration mix cylinders, containing one cylinder of Universal mix, and the other is either an NGA or RGA cylinder. ***Calibration kits contain: One box of 2 calibration mix cylinders, 2 regulators, 2 pieces of SS 1/16 in. gas sampling tubing, and 1 carry case, all packaged separately.

General Chromatography
Regulator, Carry Case and Mix Cylinders

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Integrators, Integrator Supplies, and Cables


Ink Cartridges/Integrator Paper

Integrators, Integrator Supplies, and Cables


We Can Handle Your Data Handling Needs Perfectly, Too.
At Agilent Technologies, our goal is to provide high quality columns and supplies that are the perfect fit for your instrumentation. In between our rigorous designing, manufacturing and testing, we have also taken the time to listen. We understand that your laboratory needs encompass far more than simply columns and consumables. Which is why we offer our established line of integrators, data handling supplies and cables. Information generation and its presentation has never been more important. Agilent understands that data handling supplies are indeed also laboratory supplies. So it is essential that you never run out of them at a crucial moment. We provide a means to purchase ink cartridges and integrator supplies just as conveniently as you can order our other laboratory products.

General Chromatography

Integrator Cartridges
Our replacement print cartridges are the perfect fit for your integrator. They have been designed and tested specifically for use with our Integrators, and provide the quality you've come to expect.

Agilent Integrator Ink Cartridges and Printheads


Description Agilent 3397A, 3396 Series III, 3396A and B, 3395, 3394 Printhead for 3388A and 5880A Part No. 5181-1220 19350-60540

Helpful Hint: Agilent Integrator Cartridges have an expected lifetime of 18 months, 6 months after installation.

Integrator Paper and Supplies


Paper and Supplies for the Agilent 3397A, 3396 Series III, and 3396A/B/95/94
Description Z-fold paper stand, acrylic Z-fold paper, sequentially numbered, 8.5 x 11 in. InkJet Perforated paper, 8.5 x 11 in., InkJet Perforated paper, sequentially numbered, 8.5 x 11 in., Inkjet Perforated paper, A4 size, InkJet Replacement paper roll rod Replacement paper roll brackets 2/pk 5 pk/box, 2,500 sheets 4 rolls/pk 4 rolls/pk 4 rolls/pk Unit Part No. 5062-3560 5062-3561 5181-1219 5183-2009 5181-1255 03394-20500 03396-40050

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Integrators, Integrator Supplies, and Cables


Integrator Paper/Integrators and Accessories

Integrator Paper and Supplies


Thermal Paper for the Agilent 3390/92/93
Description Thermal Printing Paper, blue print, 4.5 in. wide Unit 4 rolls/pk Part No. 5080-8800

Thermal Paper for the Agilent 338X/5830/3370


Description Z-fold paper for 3388A and 5880A, blue print 8.5 in. wide Z-fold paper for 3388A and 5880A, black print, 8.5 in. wide Z-fold paper for 3380/85A Integrators and 5830/40A terminals, blue print, 8.5 in. wide Unit 8 pks of 200 sheets each 8 pks of 200 sheets each 8 pks of 200 sheets each Part No. 5080-8778 9270-0658

General Chromatography

5080-8735

Helpful Hint: You can use sequentially numbered paper to better track your analyses. Simply record the numbers in your lab notebook for later reference.

Integrators and Accessories


Integrator Features
Integrator Agilent 3396 Series III Integrator Features BASIC programming Reintegration/replot Negative peak integration Industry standard RS-232 communications port Offers 256 kb RAM Multiple-level calibration Instrument Control (INET)

Agilent 3395B Series III Integrator Stores multiple data files in RAM Multiple-level calibrations Reintegration/replot for up to 63 levels 100% memory backup for Offers 128 kb RAM for power failure protection storing up to 20 hrs of chromatographic data at 4 Hz.

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103

Integrators, Integrator Supplies, and Cables


Integrators and Accessories/Integrator Cables Integrators and Accessories
Description 3396 Series III Integrator with BASIC programming 3395 Series III Integrator with 128 kb of RAM, general purpose signal, and remote cables
Agilent Technologies 3396 Series III

Part No. 3396C 3395B G2101A G2100A G2104A G2105A

GC Dual-channel Appak-Series III, 5890 and 6890 GC control software for the 3396 series III integrator* Integrator Starter Kit (8.0 MB data storage card, Z-fold paper stand, Z-fold paper (500 sheets), and three ink cartridges) Integrator Data Storage card, 8 MB Integrator Data Storage card, 16 MB

General Chromatography

*Requires Agilent 5890 GC communications board, option 552 or accessory 19242B

Integrator Cables
Agilent 3396 Series III Cable Sets
Description Series III Integrator General Purpose cables, signal/remote cable pair includes 35900-60630 and 03396-61031 INET interface for Agilent integrators, provides INET communications and dual-channel output, includes 1 INET cable Part No. G2108A G1553A

Cables to Connect any Agilent 3397A or 3396/95 Series III Integrator


Cable Type Instrument 6890 Series GC (non-INET) 5890 GC (non-INET) 1100 Series LC Analog G1530-60570 03396-61010 35900-60610 03394-60560 35900-60750 03396-61010 03396-60560 1090 Series LC 35900-60600 03396-60650 03396-60560 Remote Sample

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Integrators, Integrator Supplies, and Cables


Integrator Cables Cables to Connect any Agilent 3397A or 3396/95 Series III Integrator (continued)
Cable Type Instrument 1050 Series LC Analog 35900-60750 03396-61010 03396-60560 1040 diode array detector 1046A fluorescence detector 7673 automatic sampler (non-INET) All other instruments (general-purpose use) 35900-60630 03394-60540 03396-60500 35900-60600 03396-60650 35900-60750 03394-60600 Remote Sample

General Chromatography

03396-60550 03396-60560

Cables to Connect any Agilent 3394/95A and 3396A/B Integrator


Cable Type Remote 03396-61010 03394-60560 1090 Series LC 35900-60600 35900-60710 03396-60650 03396-60560 1050 Series LC 35900-60750 01046-60210 03396-60560 1040 diode array detector 1046A fluorescence detector 7673 automatic sampler General Purpose 35900-60630 35900-60920(5m) 35900-60900(5m) 03394-60540 03396-60500 www.agilent.com/chem 35900-60610 03396-60650 35900-60750 01046-60210 03395-60540 03396-60560 Instrument 6890 Series GC 5890 GC Analog G1530-60570 03396-61020 35900-60610 Sample

Helpful Hint: Need help selecting replacement cables? Call us. We'd be happy to assist you.

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Integrators, Integrator Supplies, and Cables


HPIB/GPIB/Instrument Interface Cables HPIB and GPIB Cables
Description GPIB Interface (82341C) PCI GPIB Card for Win95/98/NT HPIB Cable 2m (10833B) General Purpose Cable GPIO-Open End GPIB Interface for MS Windows and DOS HPIB Cable, 8 meter (10833G) Cable, 6890 to PC 9F/9F RS232 Crossover Ethertwist Cable, 10 ft Adapter, extends GPIB connector (10834A) Part No. G1680-63711 G1680-63715 10833B-2310 G1103-61611 G1680-63710 10833G-2310 G1530-60600 5183-4649 10834A-2310

General Chromatography

Instrument Interface Cables


Description Signal cable, general purpose analog output cable assembly, spade lugs/6 pins External event control, cable, 8 pins/spade lugs Remote start cable for general use with lug 6890 to PC via RS 232-C, 9 female/25 male user card cable General purpose binary-coded decimals cable with spade lugs Automatic liquid sampler; remote start/stop cable, 2 m 9 male/9 female HPIB cable, 2 m HPIL cable, 5 m Remote Y Cable, G1512/5890A to 3396C Remote Cable to 5890 Part No. G1530-60560 G1530-60590 35900-60670 G1530-60610 G1530-60630 G1530-60930 10833B-2310 82167-60003 G1512-60530 35900-60700

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Books and Learning Software Books, Learning Software, and CDs


PerfectFit: We Wrote the Book. Agilent Technologies offers texts, computer-based tutorials, quick reference guides, and owner manuals. For a complete list of instrument manuals and applications for CE, GC, LC, UV-Vis, Integrators, AED, and ICP-MSplease go to our website at www.agilent.com/chem. There you will find a complete up-to-date list of literature and manuals.

CBT Software
The Basics of Chromatography at Your Fingertips
Training programs for GC and LC instrument operators Take advantage of these new, self-paced tutorial programs designed for novice GC and LC instrument operators. With these informative, interactive, computer-based training programs, your laboratory staff will learn chromatography basics quickly and easily. Interactive software for maximum involvement in learning the fundamentals of chromatography. Chromatographic simulation and testing scenarios save hours of trial and error experimentation. This feature shows the results of changes on chromatograms when experimental parameters are varied. Progress reports record user's study history and document exam results for proof-of-training in audit trails. Customization by adding graphics and/or text unique to your own laboratory applications. Ability to learn at your own pace without an instructor. Available anytime at your convenience; at your home, lab or office; on your computer. Self-testing and rapid feedback ensure comprehension displaying test results and directing users to subjects needing further study. Search and find any word or phrase of text. Hot Words provide instant on-screen definitions of many important chromatographic terms. A glossary offers comprehensive listings and a book mark allows easy later return.

General Chromatography

CBT Software
Description Introduction to Gas Chromatography Introduction to Gas Chromatography, Spanish Version Introduction to HPLC Method Development in HPLC HPLC Equipment Separation Modes of HPLC Identification and Quantitation Techniques in HPLC Troubleshooting HPLC Part No. 5183-4654 5183-4727 5183-4655 5183-4657 5183-4656 5183-4659 5183-4660 5183-4658

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Books and Learning Software


Retention Time Locking (RTL) Software for the Agilent 6890 GC System
Retention time locking (RTL) software is an easy to use software product which allows users to match retention times from one capillary Agilent 6890 GC to another Agilent 6890 GC System. RTL software is integrated into the GC ChemStation. Requires Agilent Multi-technique GC ChemStation revision A.05.02 and the 6890 GC with EPC inlet.

RTL Software for the Agilent 6890 GC System


Description Retention Time Locking Software
Philip Stremple

Part No. G2080AA

General Chromatography

Books
General Chromatography Books
Description Handbook of Chemistry and Physics, Edited by C. Weast (CRC Press, latest edition) System Validation of Computerized Analysis Systems by L. Huber Validation and Qualification in Analytical Laboratories by L. Huber Part No. 9320-5798 5959-3879 5965-0036

Gas Chromatography Books


Description Detectors for Gas ChromatographyA Practical Primer by R. Buffington and M. Wilson (Agilent Technologies, 1987)
Dean Rood

Part No. 5958-9433 5183-2008 5958-9468 5182-0842 5950-3562 9320-5710 900-1010 900-1013

Gas Chromatography by I. Fowlis, 2nd edition, (John Wiley & Sons, 1995) GC InletsAn Introduction by M. S. Klee (Agilent Technologies, 1990) Electronic Pressure Control, Primer with Flocalc Disk (Agilent Technologies) High-Resolution Gas Chromatography, 3rd Edition Edited by K. J. Hyver, guest author P. Sandra (Agilent Technologies, 1989) Modern Practice of Gas Chromatography Edited by R. L. Grob (John Wiley & Sons, 1995) Analytical Gas Chromatography, 2nd Ed., W.G. Jennings, E. Mittlefehldt, P. Stremple A Practical Guide to the Care, Maintenance and Troubleshooting of Capillary GC Systems, 3rd Ed., D. Rood

Walt Jennings, Cofounder, J & W Scientific

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Books and Learning Software


Liquid Chromatography Books
Description Introduction to Modern Liquid Chromatography, 2nd Edition by L. R. Snyder and J. J. Kirkland (John Wiley & Sons, 1979) Maintaining and Troubleshooting HPLC Systems by D. J. Runser (J. Wiley Interscience, 1981) Practical HPLC Methodology and Applications by Brian A. Bidlingmeyer (John Wiley & Sons, 1993) Practical HPLC Method Development, 2nd edition by L. R. Snyder, J. J. Kirkland, and Joseph Glajch (John Wiley & Sons, 1997) Part No. 9320-5713

9320-5790 5183-2006 5183-2005

General Chromatography

Educational Primers
Description High Performance Capillary ElectrophoresisAn Introduction Good Laboratory Practice Pub. No. 5968-9963E 5968-6193E

Helpful Hint: A variety of educational primers and literature is available from Agilent at no charge. Simply call your local sales office, authorized distributor, or consult our website for more information.

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Sample Preparation
Table of Contents
Sample Preparation Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .111 Zorbax SPE Products Zorbax SPE Cartridges and 96-Well Plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .112 Zorbax SPE Formats . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .113 Zorbax C18 SPE Products . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .114115 Zorbax C18 EC SPE Products . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .116117 Zorbax C8 SPE Products . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .118 Zorbax Silica SPE Products . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .119 AccuBONDII SPE Products AccuBONDII SPE Cartridges . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .120 AccuBONDII Reversed Phase (Non-Polar) SPE Products . . . . . . . . . . . . . . . . . . .120122 AccuBONDII Normal Phase (Non-Polar) SPE Products . . . . . . . . . . . . . . . . . . . . . . . .122 AccuBONDII Normal Phase (Polar) SPE Products . . . . . . . . . . . . . . . . . . . . . . . .123124 AccuBONDII Ion Exchange SPE Products . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .124125 Comparative Herbicide Extractions Using AccuBONDII SPE Products . . . . . . . . . . . . .126 AccuBONDII Non-Silica SPE Products . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .127128 AccuBONDII Cartridge Starter Kits and Bulk SPE Sorbents . . . . . . . . . . . . . . . . . . . . .129

Sample Preparation

EvidexII SPE Products EvidexII SPE Products . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .130131 Manifolds and Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .132 Vacuum Manifolds . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .133 Adapters and Replacement Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .134 Vacuum Manifolds for 96-Well Plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .135137 AccuBONDII Empty Reservoirs and Frits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .138 CombiZorb Scavengers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .139 CombiZorb Physical Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .140141 Sample Filtration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .142147

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SPE Solutions for Your Challenging Sample Preparation Problems


Effective sample preparation can make the difference between a definitive measurement and a record of impurity-driven interferences. If samples are taken incorrectly or the sample preparation protocols do not provide good recovery or interference removal, then all the subsequent downstream steps can be negated. Agilent Technologies provides several sample preparation product lines that can help you to solve some of those challenging sample preparation problems. All of Agilents sample preparation products are manufactured under a quality program that conforms to ISO-9000 to ensure that these products meet the consistency and uniformity that you require in your everyday work.

Solid-Phase Extraction
Solid-Phase Extraction (SPE) is a technique for the cleanup and concentration of analytes from various matrices. SPE improves and simplifies separations, increases chromatography column lifetime, and improves detection limits. The following information outlines the primary steps in the application of SPE to liquid samples.

Sample Preparation

Agilent Technologies covers the full range of modern SPE requirementscartridges, 96-well plates and accessories. Zorbax SPE and AccuBONDII are SPE product families. Whatever your SPE requirements, think Agilent first.

Combinatorial Chemistry Scavengers


These products resemble SPE products except that they have chemical functionality that permits these solid phases to react with and remove undesirable reaction by-products and excess reactants from chemical reactions and combinatorial compound library syntheses. Agilent provides a large number of scavengers that will remove just about any interference encountered.

Sample Filtration
Membrane sample filters are used to clarify any samples that need further analysis or use where particulate matter may cause a problem. They are most often used for the filtration of HPLC samples to prevent damage to the injection valve or to the HPLC column inlet. Agilent provides a range of standard and economy syringe filters conveniently housed in inert polymeric housings for easy use and disposal.

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SPE
Zorbax SPE Products

Zorbax SPE Cartridges and 96-Well Plates the High Performance Choice
ZORBAX is the name you have come to know and trust for HPLC columns. Now Agilent adds Zorbax SPE productsproducts that set the same high standard to meet your most exacting sample preparation requirements. Zorbax SPE products, based on proven ZORBAX spherical base silica technology, bring you these advantages: Best reproducibility batch-to-batch, tube-to-tube, plate-to-plate Unique spherical particles that reduce pressure drop, dramatically increase flow reproducibility compared to competitive irregular SPE particles, that may contain fines and may allow replacement of vacuum manifolds with gravity-driven flow Lowest extractablespre-washed cartridges, well plates, frits, and packing materials Triple-laminated bag that ensures cleanliness and excludes oxygen and moisture before use Ultra high purity base silica, resulting in low trace metals, low acidity, and no strong irreversible adsorption of basic compounds, enhancing separation sensitivity High, repeatable recovery, leading to the most accurate quantitation with little loss of analyte on the sorbent Flow-tested to confirm fewer fines and narrow size distribution compared to sorbents from other sources, for more predictable packing density and reproducible flow characteristics Certificate of performance, based on the most rigid chromatographic tests in the industry, supplied with each lot helping you to standardize your separations Wide variety of productscartridges, 96-well plates (both fixed and flexible), and complete line of accessories, including vacuum manifolds and collection plates

Sample Preparation

ZORBAX Spherical Particles vs Competitive Irregular SPE Particles

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SPE
Zorbax SPE Products

Agilent Technologies Offers a Choice of SPE Formats from Cartridges to 96-Well SPE Plates
SPE Cartridgesthe traditional syringe barrel design cartridges in sizes from 1 mL volume to 6 mL volume packed with 25 mg to 1000 mg of packing per cartridge. The cartridges are used with standard vacuum manifolds.

SPE Cartridges

96-Well SPE Fixed Platesdesigned for high throughput SPE processing, these flowthrough design plates consist of 96 small SPE cartridges in a single polypropylene housing that fits in most standard 96-well plate vacuum manifolds. Each well has a total volume of 2 mL and weights of 25, 50 or 100 mg/well.

Sample Preparation

96-Well Fixed Plate

96-Well SPE Flexible Platesin this design, a flexible well base with 96 individual holes accommodates individual cartridges comparable in size and packing volumes to the wells in the fixed 96-well plates. You can prepare your own custom designed plates by placing individual cartridges in the base plate holes. Individual cartridges come in packs of 100. The flow-through flexible plates are useful for method development since they allow you to test a variety of phases, masses and volumes to optimize their choice of SPE conditions. The small SPE flexible cartridges can also be used with standard vacuum manifold system using a small Luer adapter. Each flexible cartridge has a total volume of 1 or 2 mL and weights of 25, 50 or 100 mg.
Flexible 96-Well Plate Configuration

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SPE
Zorbax SPE Products

Zorbax C18 SPE Products


Reversed phase mode Octadecylsilane (ODS, C18) Monomeric Bonding (11.1% carbon loading) Non-endcapped, enhanced polar interactions relative to endcapped (EC) version Spherical silica, reduced pressure, more consistent flow 80 Angstrom average pore size; 50 m average particle diameter Recommended as general purpose SPE phase for both polar and non-polar analytes Retention mechanisms: - Non-polar - Polar - Cation exchange by virtue of ionized silanols

Structure of C18 octadecylsilane (ODS), covalently bonded to the surface of a silica particle

Sample Preparation

Description
C18 Cartridges

Unit 100/pk 100/pk 100/pk 50/pk 50/pk 30/pk 30/pk

Part No. 5184-3588 5184-3589 5184-3590 5184-3591 5184-3592 5184-3593 5184-3594

25 mg, 1 mL 50 mg, 1 mL 100 mg, 1 mL 200 mg, 3 mL 500 mg, 3 mL 500 mg, 6 mL 1000 mg, 6 mL
C18 96-Well SPE Plates

25 mg/well 25 mg/well 50 mg/well 50 mg/well 100 mg/well 100 mg/well


C18 Flexible Plate Cartridges*

5185-5721 5/pk 5/pk 5/pk 5185-5722 5185-5723 5185-5724 5185-5725 5185-5726

25 mg, 1 mL 50 mg, 1 mL 100 mg, 1 mL 100 mg, 2 mL

100/pk 100/pk 100/pk 100/pk

5185-5745 5185-5746 5185-5747 5185-5769

*Requires base plate (P/N 5185-5787) for 96-well operation

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Zorbax SPE Prodcuts

Zorbax C18 SPE Products


Application Example
Analysis of Naproxen in Serum: Preparation with a Zorbax SPE C18 Cartridge Solid Phase Extraction Method CARTRIDGE: PRE-TREAT: LOAD: WASH: ELUTE: INTERNAL STANDARD: RECOVERY: 100 mg, 1 mL Zorbax C18 (P/N 5184-3590, 100/pk) 1% Acetic acid in methanol, deionized water 1.0 mL sample 4% Isopropyl alcohol in 100 mM formic acid, deionized water 50% Acetonitrile in 40 mM ammonium acetate Nitrobenzene in methanol
Comparison of Naproxen-spiked serum before (A) and after (B) extraction using the Zorbax SPE C18 cartridge and analysis by HPLC. Note substantial reduction of protein extractables, providing better resolution and quantitation of the drug. HPLC column lifetime was extended due to cleaner sample injections. HPLC Column used: ZORBAX SB-C8, 4.6 x 150 mm (P/N: 863953-906)

Sample Preparation

HIGH LOAD (10 g): X = 99.25%, n = 3 LOW LOAD (2 g): X = 102.2%, n = 3

Application Example
Analysis of Ranitidine in Serum: Preparation with a Zorbax SPE C18 Cartridge Solid Phase Extraction Method CARTRIDGE: PRE-TREAT: LOAD: WASH: ELUTE: INTERNAL STANDARD: RECOVERY: 100 mg, 1 mL Zorbax C18 (P/N 5184-3590, 100/pk) Methanol, deionized water 1.0 mL sample Deionized water Methanol Prednisolone in deionized water X = 100%, n = 3
Comparison of Ranitidine-spiked serum before (A) and after (B) extraction using the Zorbax SPE C18 cartridge and analysis by HPLC. SPE can help to lower interferences and provide enhanced resolution for routine drug testing. HPLC Column Used: ZORBAX Bonus RP, 4.6 x 150 mm (P/N: 883688-904)

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Zorbax SPE Products

Zorbax C18 EC SPE Products


Reversed phase mode Octadecylsilane (ODS, C18) Monomeric Bonding (14.8% carbon loading) Endcapped, reduced polar interactions relative to non-endcapped version Spherical silica, reduced pressure, more consistent flow 80 Angstrom average pore size; 50 m average particle diameter Recommended for non-polar analytes Retention mechanisms: - Non-polar

Structure of C18 silane and trimethyl silyl endcapping group, covalently bonded to the surface of a silica particle

Description
C18 EC Cartridges

Unit 100/pk 100/pk 100/pk 50/pk 50/pk 30/pk 30/pk

Part No. 5184-3595 5184-3596 5184-3597 5184-3598 5184-3599 5184-3600 5185-5701

25 mg, 1 mL

Sample Preparation

50 mg, 1 mL 100 mg, 1 mL 200 mg, 3 mL 500 mg, 3 mL 500 mg, 6 mL 1000 mg, 6 mL
C18 EC 96-Well Plates

25 mg/well 25 mg/well 50 mg/well 50 mg/well 100 mg/well 100 mg/well


C18 EC Flexible Plate Cartridges*

5185-5727 5/pk 5/pk 5/pk 5185-5728 5185-5729 5185-5730 5185-5731 5185-5732

25 mg, 1 mL 50 mg, 1 mL 100 mg, 1 mL 100 mg, 2 mL

100/pk 100/pk 100/pk 100/pk

5185-5748 5185-5749 5185-5750 5185-5770

*Requires base plate (P/N 5185-5787) for 96-well operation

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Zorbax SPE Products

Zorbax C18 EC SPE Products


Application Example
Analysis of Clozapine and Metabolites in Plasma: Preparation with a Zorbax SPE C18 EC Cartridge* Solid Phase Extraction Method CARTRIDGE: PRE-TREAT: LOAD: WASH: ELUTE: INTERNAL STANDARD: RECOVERY: 100 mg, 1 mL Zorbax C18 EC (P/N 5184-3597, 100/pk) Methanol, deionized water Plasma, flurazepam, deionized water Deionized water, methanol/water Methanol Flurazepam
Analysis of clozapine and its metabolites using the Zorbax SPE C18 EC cartridge.

Sample Preparation

Clozapine (200 g/mL): X = 103%, n = 10 LOW LOAD (2 g): Norclozapine (200 g/mL) X = 111, n = 10
* Analysis of Clozapine: Zorbax SPE C18 EC Cartridge courtesy of Dr. David Freeman, Dept. of Pharmacology and Toxicology, J. P. Robarts Research Institute, London, Ontario, Canada

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Zorbax SPE Products

Zorbax C8 SPE Products


Reversed phase mode Octylsilane (ODS, C8) Monomeric Bonding (8.3% carbon loading) Non-endcapped, polar interactions Spherical silica, reduced pressure, more consistent flow 80 Angstrom average pore size; 50-m average particle diameter Recommended as a general purpose sorbent for both polar and non-polar analytes less retentive compared to Zorbax C18 (SPE) Retention mechanisms: - Non-polar - Polar - Cation exchange by virtue of ionized silanols

Structure of C8 octyl silane, covalently bonded to the surface of a silica particle

Description

Unit 100/pk 100/pk 100/pk 50/pk 50/pk 30/pk 30/pk

Part No. 5184-3574 5184-3575 5184-3576 5184-3577 5184-3578 5184-3579 5184-3580

Sample Preparation

C8 Cartridges

25 mg, 1 mL 50 mg, 1 mL 100 mg, 1 mL 200 mg, 3 mL 500 mg, 3 mL 500 mg, 6 mL 1000 mg, 6 mL
96-Well SPE Plates, 5/pk

C8 96-Well SPE Plates

25 mg/well 25 mg/well 50 mg/well 50 mg/well 100 mg/well 100 mg/well


C8 Flexible Plate Cartridges*

5185-5709 5/pk 5/pk 5/pk 5185-5710 5185-5711 5185-5712 5185-5713 5185-5714

C8, 25 mg, 1 mL C8, 50 mg, 1 mL C8, 100 mg, 1 mL C8, 100 mg, 2 mL

100/pk 100/pk 100/pk 100/pk

5185-5739 5185-5740 5185-5741 5185-5796

*Requires base plate (P/N 5185-5787) for 96-well operation.

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Zorbax SPE Products

Zorbax Silica SPE Products


Normal phase (adsorption) mode Unbonded, activated silica gel adsorbent Polar interactions with silanols via hydrogen bonding Recommended as adsorbent for polar analytes in non-polar matrices Retention mechanisms: - Non-polar matrix, polar analytepolar retention mechanism - Aqueous matrix, cationic analyte, intermediate pHcation exchange retention mechanism

Chemical structure of silanol groups on the surface of a silica particle

Description
Silica Cartridges

Unit 100/pk 100/pk 100/pk 50/pk 50/pk 30/pk 30/pk

Part No. 5185-5702 5185-5703 5185-5704

25 mg, 1 mL 50 mg, 1 mL 100 mg, 1 mL 200 mg, 3 mL 500 mg, 3 mL 500 mg, 6 mL 1000 mg, 6 mL
Silica 96-Well SPE Plates

Sample Preparation

5185-5705 5185-5706 5185-5707 5185-5708

25 mg/well 25 mg/well 50 mg/well 50 mg/well 100 mg/well 100 mg/well


Silica Flexible Plate Cartridges*

5185-5733 5/pk 5/pk 5/pk 5185-5734 5185-5735 5185-5736 5185-5737 5185-5738

25 mg, 1 mL 50 mg, 1 mL 100 mg, 1 mL 100 mg, 2 mL

100/pk 100/pk 100/pk 100/pk

5185-5751 5185-5752 5185-5753 5185-5774

*Requires base plate (P/N 5185-5787) for 96-well operation.

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AccuBOND" SPE Products

AccuBONDII SPE Cartridges A Versatile Sample Preparation Family


The AccuBONDII name on SPE cartridges indicates high quality in a wide selection of phases for use in routine analyses that call for consistent results with higher sample volumes. Based on high purity, irregularly shaped silica packing, AccuBONDII cartridges bring you these valuable characteristics: High quality, low extractables for dependable separations Solvent pre-washed sorbents, frits, and cartridge bodies Wide variety of phases Larger mass cartridges for extraction of environmental samples Suitable for general SPE method development Specific phases for routine extractions of drugs of abuse from biological fluids Attractive pricing for routine, high volume laboratory use

Sample Preparation

AccuBONDII Reversed Phase (Non-polar) SPE Products


Reversed phase sorbents are non-polar, and used to retain (extract) non-polar analytes. For reversed phase sorbents, solvent strength increases as the solvent becomes more non-polar.

AccuBONDII C18 Products


AccuBONDII C18 products are based on bonded, reversed phase (octadecylsilane), irregular silica gel (silica) particles. This non-polar, non-endcapped sorbent provides additional polar interactions associated with surface silanol groups. It also enhances retention of basic compounds compared with the corresponding endcapped sorbent. It is recommended as a general purpose SPE phase for both polar and non-polar analytes.

Structure of octadecyl silane, covalently bonded to the surface of a silica particle

Description
Octadecylsilane (ODS) (C18)

Unit 100/pk 50/pk 50/pk 30/pk 30/pk

Part No. 188-1310 188-1320 188-1350 188-1356 188-1360

100 mg, 1 mL 200 mg, 3 mL 500 mg, 3 mL 500 mg, 6 mL 1000 mg, 6 mL

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AccuBOND" SPE Products AccuBONDII Octyl (C8) SPE Products
AccuBONDII C8 products are based on bonded, reversed phase (octylsilane), irregular silica gel (silica) particles. This non-polar, non-endcapped sorbent is recommended as a general purpose SPE phase for non-polar analytes. It is slightly less retentive than AccuBONDII C18 products. For basic analytes, octyl sorbents often can increase the extraction efficiency and enhance their purity.
Structure of octyl (C8) silane, covalently bonded to the surface of a silica particle

Description
Octyl (C8)

Unit 100/pk 50/pk 50/pk 30/pk 30/pk

Part No. 188-0310 188-0320 188-0350 188-0356 188-0360

100 mg, 1 mL 200 mg, 3 mL 500 mg, 3 mL 500 mg, 6 mL 1000 mg, 6 mL

Sample Preparation

AccuBONDII Ethyl (C2) SPE Products


AccuBONDII Ethyl (C2) SPE products are manufactured using a trifunctional silane bonded to irregular silica gel (silica) particles. They are not endcapped, allowing additional polar interactions with surface silanol groups. The short chain ethyl group provides less retention than the longer C8 and C18 chains, where retention is primarily based on non-polar interactions. The stronger interactions of this phase with basic and polar analytes can provide additional extraction selectivity.

Structure of ethyl (C2) silane, covalently bonded to the surface of a silica particle

Description
Ethyl (C2)

Unit 100/pk 50/pk 50/pk 30/pk 30/pk

Part No. 5185-5811 5185-5812 5185-5813 5185-5814 5185-5815

100 mg, 1 mL 200 mg, 3 mL 500 mg, 3 mL 500 mg, 6 mL 1000 mg, 6 mL

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AccuBOND" SPE Products AccuBONDII Phenyl SPE Products
AccuBONDII Phenyl SPE products are based on bonded, reversed phase (phenyl), irregular silica gel (silica) particles. This phase exhibits additional polar secondary interactions, which can enhance retention of basic compounds. It exhibits a different selectivity compared with the octadecyl and octyl phases when both aromatic and nonaromatic compounds are being extracted.

Structure of phenyl silane, covalently bonded to the surface of a silica particle

Description
Phenyl

Unit 100/pk 50/pk 50/pk 30/pk 30/pk

Part No. 188-0510 188-0520 188-0550 188-0556 188-0560

100 mg, 1 mL 200 mg, 3 mL 500 mg, 3 mL 500 mg, 6 mL 1000 mg, 6 mL

Sample Preparation

AccuBONDII Normal Phase (Non-polar) SPE Products


Normal phase sorbents are polar and used to retain (extract) polar analytes. For normal phase sorbents, solvent strength increases as the solvent becomes more polar.

AccuBONDII Silica SPE Products


AccuBONDII Silica SPE products are based on unbonded, activated irregular silica gel (silica) particles. The primary interaction available is polar (e.g., hydrogen bonding). The silanol groups are ionizable, so it can be used as a weak cation exchanger at intermediate pH values.

Structure of silanol groups on the surface of a silica particle

Description
Silica

Unit 100/pk 50/pk 50/pk 30/pk 30/pk

Part No. 188-0110 188-0120 188-0150 188-0156 188-0160

100 mg, 1 mL 200 mg, 3 mL 500 mg, 3 mL 500 mg, 6 mL 1000 mg, 6 mL

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AccuBOND" SPE Products

AccuBONDII Normal Phase (Polar) SPE Products


AccuBONDII Cyano (CN) SPE Products
AccuBONDII Cyano (CN) SPE products are based on irregular silica gel (silica) particles. This polar, bonded sorbent can exhibit both polar and non-polar interactions. It can be used as a non-polar sorbent for extraction of both polar and non-polar molecules from aqueous samples, and for extraction of polar molecules from relatively non-polar solvents.

Structure of cyano silane, covalently bonded to the surface of a silica particle

Description
Cyano (CN)

Unit 100/pk 50/pk 50/pk 30/pk 30/pk

Part No. 188-0610 188-0620 188-0650 188-0656 188-0660

100 mg, 1 mL 200 mg, 3 mL 500 mg, 3 mL 500 mg, 6 mL 1000 mg, 6 mL

Sample Preparation

AccuBONDII Diol SPE Products


AccuBONDII Diol SPE products are based on irregular silica gel (silica) particles. This polar, bonded sorbent can exhibit both polar and weak non-polar interactions, depending on the cartridge preparation and sample matrix. While, with appropriate cartridge conditioning, it can be used as a non-polar sorbent to extract relatively non-polar molecules from aqueous samples, it is more frequently used to extract polar molecules from relatively non-polar solvents using hydrogen bonding interactions.

Structure of diol silane, covalently bonded to the surface of a silica particle

Description
Diol

Unit 100/pk 50/pk 50/pk 30/pk 30/pk

Part No. 188-0710 188-0720 188-0750 188-0756 188-0760

100 mg, 1 mL 200 mg, 3 mL 500 mg, 3 mL 500 mg, 6 mL 1000 mg, 6 mL

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AccuBOND" SPE Products AccuBONDII Amino (NH2) SPE Products
AccuBONDII Amino (NH2) SPE products are based on irregular silica gel (silica) particles. This dual purpose sorbent can act either as a polar phase or weak anion exchanger. When conditioned with a non-polar solvent, e.g., hexane, it can hydrogen bond with any molecule containing OH, NH, or SH functional groups. In an aqueous environment with pH 7.8 or less, it can function as a weak anion exchanger.

Structure of amino (NH2) silane, covalently bonded to the surface of a silica particle

Description
Amino (NH2)

Unit 100/pk 50/pk 50/pk 30/pk 30/pk

Part No. 188-1010 188-1020 188-1050 188-1056 188-1060

100 mg, 1 mL 200 mg, 3 mL 500 mg, 3 mL 500 mg, 6 mL

Sample Preparation

1000 mg, 6 mL

AccuBONDII Ion Exchange SPE Products


Ion exchange phases are more dependent on pH, ionic strength, and counter-ion strength than on solvent strength. These phases depend on ionic interactions as the primary retention mechanism.

AccuBONDII Strong Anion Exchange (SAX) SPE Products


AccuBONDII SAX SPE products are based on irregular silica gel (silica) particles. This strong anion exchanger is used to extract compounds capable of carrying a negative charge from both aqueous and non-aqueous solutions. They are ideally suited to extraction of weak acids. Nominal capacity 0.6 meq/g.

Structure of SAX silane, covalently bonded to the surface of a silica particle

Description
Strong Anion Exchange (SAX)

Unit 100/pk 50/pk 50/pk 30/pk 30/pk

Part No. 188-1610 188-1620 188-1650 188-1656 188-1660

100 mg, 1 mL 200 mg, 3 mL 500 mg, 3 mL 500 mg, 6 mL 1000 mg, 6 mL

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AccuBOND" SPE Products AccuBONDII Strong Cation Exchange (SCX) SPE Products
AccuBONDII SCX SPE products are based on irregular silica gel (silica) particles. These strong cation exchange sorbents are used to extract positively charged basic compounds. This benzene-sulphonic acid-based sorbent has significant non-polar secondary interactions, with nominal capacity 0.3 meq/g.

Structure of SCX silane, covalently bonded to the surface of a silica particle

Description
Strong Cation Exchange (SCX)

Unit 100/pk 50/pk 50/pk 30/pk 30/pk

Part No. 188-1510 188-1520 188-1550 188-1556 188-1560

100 mg, 1 mL 200 mg, 3 mL 500 mg, 3 mL 500 mg, 6 mL 1000 mg, 6 mL

Sample Preparation

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AccuBOND" SPE Products

Comparative Herbicide Extractions Using SPE Products


Application Example
Detection of triazine herbicides can be critical to food safety. Analysis of these persistent chemicals in various matrices is simplified by the use of AccuBONDII SPE sample preparation. Examples in soilcontaining a large number of charged species, muscle tissuewith large amounts of relatively non-polar components such as lipids and corn oilwith glycerides and fatty acids that are non-polar compared to the triazines show effective separation of the three prominent triazine species. Refer to the chromatograms to see the chromatographic results. Analysis of Triazines in a Variety of Samples: Preparation with AccuBONDII SPE Cartridges

General Structure of Triazine Herbicides

Solid Phase Extraction Method

Sample Preparation

Results in Chromatogram Soil CARTRIDGE EXTRACTION


Soil

Muscle Tissue ODS (C18) Homogenized in methanol Methanol Diluted with water Water

Corn Oil Diol None Methanol, hexane Diluted with hexane Hexane

SCX Shaken in acetonitrile Acetic acid Diluted with acetic acid Acetic acid, acetonitrile, water, 0.1 M K2HPO4 Acetonitrile/ K2HPO4

PRE-TREAT LOAD WASH

ELUTE

Methanol

Methanol

Key to Chromatograms
Muscle Tissue

Peak 1 2 3 Simazine Atrazine Propazine

R1 CH2CH3 CH2CH3 CH(CH3)2

R2 CH2CH3 CH(CH3)2 CH(CH3)2

Corn Oil

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AccuBOND" SPE Products

AccuBONDII Non-Silica SPE Products


Non-silica-based SPE phases have varying degrees of polarity and surface acidity or basicity. They are primarily used to retain polar analytes. For these phases, solvent strength generally increases as the solvent becomes more polar.

AccuBONDII Alumina A (Acidic) SPE Products


AccuBONDII Alumina A sorbents, with 50-200 m particle size, can adsorb molecules by interaction with the aluminum metal center, hydrogen bonding with the surface hydroxyl groups, or ion exchange if the surface carries a charge. Acid washing results in a surface with decreased capacity for basic compounds.

Description
Alumina A (Acidic)

Unit 100/pk 50/pk 50/pk 30/pk 30/pk

Part No. 188-2110 188-2120 188-2150 188-2156 188-2160

100 mg, 1 mL 200 mg, 3 mL 500 mg, 3 mL 500 mg, 6 mL 1000 mg, 6 mL

Sample Preparation

AccuBONDII Alumina B (Basic) SPE Products


AccuBONDII Alumina B sorbents, with 50-200 m particle size, can adsorb molecules by interaction with the aluminum metal center, hydrogen bonding with the surface hydroxyl groups, or ion exchange if the surface carries a charge. Washing with a basic solution results in a net negative charge, allowing retention of certain compounds as cations on the surface or by specific interaction with the center.

Description
Alumina B (Basic)

Unit 100/pk 50/pk 50/pk 30/pk 30/pk

Part No. 188-2210 188-2220 188-2250 188-2256 188-2260

100 mg, 1 mL 200 mg, 3 mL 500 mg, 3 mL 500 mg, 6 mL 1000 mg, 6 mL

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AccuBOND" SPE Products AccuBONDII Alumina N (Neutral) SPE Products
AccuBONDII Alumina N sorbents, with 50-200 m particle size, can adsorb molecules by interaction with the aluminum metal center, hydrogen bonding with the surface hydroxyl groups, or by ion exchange if the surface carries a charge. The neutralized surface allows interaction with compounds whose heteroatoms are electronegative (e.g., N, O, P, S) or with an electron-rich, highly aromatic structure.

Description
Alumina N (Neutral)

Unit 100/pk 50/pk 50/pk 30/pk 30/pk

Part No. 188-2310 188-2320 188-2350 188-2356 188-2360

100 mg, 1 mL 200 mg, 3 mL 500 mg, 3 mL 500 mg, 6 mL 1000 mg, 6 mL

Sample Preparation

AccuBondII Florisil SPE Products


AccuBONDII Florisil is a selective synthetic magnesia-silica adsorbent, specially processed to give consistent results when used for column cleanup and separation of chlorinated pesticide residues prior to identification and measurement of the pesticide by gas, thin layer, or paper chromatography.

Description
Florisil

Unit 100/pk 50/pk 50/pk 30/pk 30/pk

Part No. 188-2410 188-2420 188-2450 188-2456 188-2460

100 mg, 1 mL 200 mg, 3 mL 500 mg, 3 mL 500 mg, 6 mL 1000 mg, 6 mL

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AccuBOND" SPE Products

AccuBONDII Cartridge Starter Kits and Bulk SPE Sorbents


AccuBONDII sorbents are available in bulk, along with a selection of reservoirs and frits, for those who wish to pack their own SPE cartridges or to perform flash chromatography.

Description
SPE Starter Kits 10 each in 100 mg, 1 mL configuration

Part No.
AccuBONDII Bulk SPE Sorbents

Kit A (Reversed Phase) C2, C8, C18, CN, and Phenyl Phases Kit B (Normal Phase) Silica, Amino, Diol, and CN Phases Kit C (Ion-Exchange Phase) SCX, SAX, and Amino Phases

009-4003 009-4004 009-4005

Sample Preparation

Description
AccuBOND Bulk SPE Sorbents
II

Unit Bottle of 25 g Bottle of 25 g Bottle of 25 g Bottle of 25 g Bottle of 25 g Bottle of 25 g Bottle of 25 g Bottle of 25 g Bottle of 25 g Bottle of 25 g Bottle of 25 g Bottle of 25 g Bottle of 25 g Bottle of 25 g

Part No. 189-1302 189-0302 5185-5816 189-0502 189-0102 189-0702 189-0602 189-1002 189-1602 189-1502 189-2102 189-2202 189-2302 189-2402

ODS (C18) Octyl (C8) Ethyl (C2) Phenyl Silica Diol Cyano (CN) Amino (NH2) SAX SCX Alumina A Alumina B Alumina N Florisil

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Evidex" SPE Products Application Example
Opiates in Urine by GC/MS Conditions COLUMN: CARRIER: OVEN: INJECTOR: DETECTOR: DB-5ms-EVDX (Agilent Technologies P/N 128-8522) Helium at 40 cm/sec 65C for 1 min, 65-325C at 20 degrees/min Splitless, 250C MSD, 30C transfer line

EvidexII SPE Products


EvidexII SPE CartridgesOptimum Separation for Drugs-of-Abuse Testing
Effective preparation is an important step in building compelling evidence in drugs-of-abuse cases. The EvidexII SPE cartridgesuitable for all necessary methodssupports the kind of reliable, accurate separation that you need, with characteristics like: Cartridge designed for NIDA-5 drug classes (NIDANational Institute of Drug Abuse is now called SAMSHA Substance Abuse and Mental Health Services Administration) Amphetamine/Methamphetamine PCP Benzoylecgonine Codeine/Morphine THC-COOH (Marijuana) Step-by-step instructions included, for testing consistency Accurate, reproducible results (< 5% RSD)

Sample Preparation

Codeine 30-ng

Morphine 30-ng

Proprietary bonding chemistry (mixed phase) Tested with actual drugs of abuse to ensure lot-to-lot reproducibility, high recovery, and clean extracts with low background Forgiving of minor errors of volumes and concentrations of reagents Specific GC Evidex capillary columns for optimized separations of drug classes
Description 200 mg, 3 mL Unit 50/pk 30/pk Part No. 188-2920 188-2946

Morphine

Codeine

400 mg, 6 mL

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Evidex" SPE Products

Drugs of Abuse Gas Chromatography Columns


DB-EVDX
Specially configured and tested for drugs of abuse confirmation DB-1-EVDX: 100% Dimethylpolysiloxane DB-5ms-EVDX: Equivalent to (5%-Phenyl)- methylpolysiloxane DB-17-EVDX: (50%-Phenyl)- methylpolysiloxane Consistent rentention and peak shape. Test mix includes: caffeine. gluthethimides, lidocaine, phenobarbital, EDDP, Methaqualone, methadone, cocaine, desipramine, carbamazepine Low bleed for GC/MS analysis Bonded and cross-linked Solvent rinsable

Sample Preparation

Helpful Hint: Please contact Technical Support for the test mix specifications.

Phase

ID (mm)

Length (m)

Film (m)

Temp Limits (C)

Part No.

DB-1-EVDX DB-5ms-EVDX DB-17-EVDX

0.20 0.20 0.20

25 25 25

0.33 0.33 0.33

-60 to 325/350 -60 to 325/350 40 to 280/300

128-8122 128-8522 128-8722

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Manifolds and Accessories

Manifolds and Accessories for Convenient, Successful Sample Preparation


Agilent manifolds and accessories complement the quality of ZORBAX and AccuBONDII sorbents. Flexible configurations and availability as complete assemblies or individual components mean that the user has the necessary capability at any stage from method development to high throughput operation.

Cartridge Vacuum Manifold Processing Stations


Vacuum manifold processing stations for individual cartridges are suitable for use from method development to lower volume production. They can be used with individual cartridges or flexible well-plate cartridges. Shown here is a 10-port manifold assembly, (P/N 5185-5754) with all inputs plugged. Your capability is enhanced with features like: Available for either 10 or 20 positions Tank constructed of chemically resistant, vacuum-safe glass

Sample Preparation

Vacuum Manifold Processing Station P/N 5185-5754

Ultra high molecular weight polyethylene lid that ensures excellent solvent resistance Vacuum gauge with coarse and fine control valves for monitoring and adjustment of vacuum levels, plus a safety release valve Choice of height-adjustable racks to accommodate a variety of collection vessels (rack options to fit 10, 12 and 16 mm tube diameters); standard rack for 16 mm tubes Compatible with standard Luer-tipped SPE and filtration columns Individually controlled PTFE stopcocks, permitting flow control at each port position (optional) Clearly marked ports and racks to ensure correct positioning of collection vessels

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Manifolds and Accessories
Vacuum Manifolds for Individual Cartridges
Description
10-Port Manifolds for Cartridges

Part No. 5185-5754 5185-5755 5185-5756 5185-5757 5185-5760 5185-5761 5185-5762


Stacking Adapter (P/N 5185-5794)

Vacuum manifold assembly, 10 port, w/16 mm rack (adjustable)* Rack, optional, 10 x 10 mm, adjustable height Rack, optional, 10 x 12 mm, adjustable height Rack, optional, 10 x 16 mm, adjustable height Lid, 10 position for 10-port vacuum manifold Lid gasket for 10-port vacuum manifold Glass tank for 10-port vacuum manifold

Luer adapters (P/N 5185-5789) allow use of flexible well-plate cartridges in the 10-or 20-port manifold.

Sample Preparation

* Vacuum manifold can be used with Flexible Plate Cartridges, with addition of Luer adapter for 25/pk (P/N 5185-5789)

Needle Retainer (P/N 5185-5764)

Stopcock Valve (P/N 5185-5758)

Multi-stage preparations are straightforward, with use of the accessories shown.

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Manifolds and Accessories

Adapters and Replacement Parts


A full selection of adapters allows stacking cartridges for multi-step separation or use of flexible cartridges on 10 or 20-port manifolds. Parts are available for replacement of worn or contaminated parts and subassemblies.

Description
20-Port Manifolds for Cartridges

Unit

Part No. 5185-5765 5185-5766 5185-5767 5185-5768 5185-5771 5185-5772 5185-5773

Vacuum manifold assembly, 20 port, w/16 mm rack (adjustable)* Rack, optional, 20 x 10 mm, adjustable height Rack, optional, 20 x 12 mm, adjustable height

Sample Preparation

The 20-port manifold assembly (P/N 5185-5765) is shown with all three sizes of SPE cartridge, illustrating its adaptability.

Rack, optional, 20 x 16 mm, adjustable height Lid, 20 position for 20-port vacuum manifold Lid gasket for 20-port vacuum manifold Glass tank for 20-port vacuum manifold
Parts and Disposables for Individual Cartridge Manifolds

Stopcock valve for vacuum manifold


Cartridge contents are retained and protected on top by use of the appropriately sized cap (available for 1 mL, 3 mL, and 6 mL cartridges) and on the bottom by a Luer cap (P/N 5185-5795).

10/pk** 10/pk** 10/pk** 10/pk** 100/pk 100/pk 100/pk 10/pk 100/pk

5185-5758 5185-5759 5185-5763 5185-5764 5185-5791 5185-5792 5185-5793 5185-5794 5185-5795

Stainless steel needles for vacuum manifold Port seal plugs for vacuum manifold Needle retainer for 10-port vacuum manifold Caps for 1 mL SPE cartridges Caps for 3 mL SPE cartridges Caps for 6 mL SPE cartridges Adapters, stacking 1, 3, or 6 mL cartridges Luer caps (fit all cartridge sizes)

*Vacuum manifold can be used with Flexible Plate Cartridges, with addition of Luer adapter (P/N 5185-5789) **For a 20 port manifold, order 2 packs of 10.

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SPE
Manifolds and Accessories

Vacuum Manifolds for 96-Well Plates


96-Well Vacuum Manifold Processing Stations
These stations allow configurations that are usable for both method development and routine testing. Flexible 96-well trays are designed for easy configuration with the selection of cartridges you need for method development. Fill a row, column, or other arrangement, and cap the rest. Fixed 96-well trays support higher volume, automated production. All wells are packed with the same sorbent and mass as the comparable flexible well cartridge, ensuring uniform, predictable separations. Vacuum manifolds for 96-well trays are designed to allow both convenient method development and high throughput operation. The base accommodates lids that hold either a userdefined selection of flexible cartridges, or fixed format 96-well plates with each well loaded with the same sorbent. The processing station can accommodate either fixed position plates or flexible format plates with quality features like: Constructed with polypropylene base and polyethylene lid (choose flexible and/or fixed format lid) On-off switch, vacuum gauge, and fine vacuum control valve for full control Disposable reservoir tray to collect excess sample and wash solvents Allows spacer inserts to be placed into the base so that collection plates of differing heights can be processed (both deep well and standard microtiter plates); ensures maximum penetration of the SPE plate into the collection plate and reduces possible well-to-well contamination Solvent-resistant manifold lid gasket Small footprint
1 9 8 7 3 6 1 4 5 2

The system is shown here with a fixed format tray, comprising: 1. Basecomplete assembly (P/N 5185-5797) Replacement parts: 2. Vacuum gauge (P/N 5185-5786) 3. On/off valve (P/N 5185-5785) 4. Needle valve (P/N 5185-5783) 5. Vacuum outlet (P/N 5185-5784) The setup shown includes: 6. Lid for fixed well manifold (P/N 5185-5798) 7. Fixed 96-well plate

Sample Preparation

Note: Must order base (P/N 5185-5797) and one lid, either fixed plate lid (P/N 5185-5798) or flexible plate lid (P/N 5185-5799). See page 137 for prices.

A 96-well manifold system is shown here equipped for flexible format plates, comprising: 1. Basecomplete assembly (P/N 5185-5797) 8. Lid for flexible format manifold (P/N 5185-5799) 9. Collection plate

Vacuum Manifolds for 96-Well Fixed or Flexible Plates


Description Base for 96-well vacuum manifold (includes vacuum gauge, needle valve, etc.). Select the appropriate lid, etc., for fixed (5185-5798) or flexible format (5185-5799). See page 137. Part No. 5185-5797

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Manifolds and Accessories
Parts and Disposables for 96-Well Plates
Description
1 2 3

Unit 32/pk

Part No. 5042-1387 5065-4402

96-well deep well collection plates, 1 mL, polypropylene 96-well collection plates with glass inserts, caps, and septa, pre-assembled, 0.35 mL 96-well collection plates, 0.5 mL, polypropylene 96-well collection plates, 0.5 mL, polypropylene Base O ring for 96-well plate manifolds Base plate/flexible format 96-well cartridges Closing mats Collection plate spacer for Agilent 1.0 mL deep well, 12 mm

10/pk 120/pk

5042-1386 5042-1385 5185-5779 5185-5787

Collection plates for 96-well manifolds: 1. Pre-assembled system with glass inserts, caps, and septa (P/N 5065-4402) 2. Deep well collection plate (P/N 5042-1387) 3. 0.5 mL collection plate (P/N 5042-1386)

50/pk

5042-1389 5185-5775 5185-5781

Sample Preparation

Collection plate spacer for microtiter plate and Agilent 0.5 mL shallow well plate, 29 mm

Base O-ring (P/N 5185-5779)

Collection plate, showing 96-position closing mat (P/N 5042-1389)

Collection plate spacer, in sizes to match the collection plate used (P/N 5185-5775)

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Manifolds and Accessories
Vacuum Manifolds for Individual Cartridges
Description Collection plate spacer for most industrystandard deep well plates, 2 mm Disposable reservoir tray, 96-well manifold Lid for 96-well fixed well vacuum manifold Lid for 96-well flexible well vacuum manifold Lid gasket for 96-well plate manifold Luer adapters for 96-well flexible cartridge Needle valve for 96-well manifold On/off valve for 96-well manifold Strip of 8 base plate Luer plugs Vacuum gauge for 96-well manifold Vacuum outlet (Ni plated) 96-well manifold Well insertion/removal tool for flexible plate cartridge 50/pk 25/pk 25/pk Unit Part No. 5185-5780 5185-5782 5185-5798 5185-5799 5185-5778 5185-5789 5185-5783 5185-5785 5185-5788 5185-5786 5185-5784 5185-5790
Lid for flexible format manifold (P/N 5185-5799) Disposable reservoir tray (P/N 5185-5782)

Sample Preparation

SPE Accessories for Discontinued J&W Manifold 600-4000


Description Vacuum gauge 1/8 in. NPT, 0-30 in. Hg Port sealing plugs Lid gasket Luer fittings, female Teflon tubing guides 10/pk 12/pk 10/pk Unit Part No. 715-0006 5185-5763 701-4000 5185-5764 701-4005
Luer adapters for flexible cartridges for use in standard 10 and 20-port vacuum manifold (P/N 5185-5789)

Strip of base plate Luer plugs for capping unused base plate holes in flexible format configuration (P/N 5185-5788)

Well insertion/removal tool for flexible plate cartridges (P/N 5185-5790)

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SPE
AccuBOND" Reservoirs and Frits

AccuBONDII Empty Reservoirs and Frits


Empty reservoirs and separate or integrated frits are available if you would like to prepare your own solid phase extraction cartridges. By purchasing bulk SPE sorbents, page 129, you can pack different masses of sorbent, mixed phases or any combination that you desire. The larger volume reservoirs can be used for low pressure or flash chromatography.

Description Empty reservoirs, Luer fitting on bottom, 3 mL Empty reservoirs, Luer fitting on bottom, 6 mL Empty reservoirs, Luer fitting on bottom, 25 mL Empty reservoirs, Luer fitting on bottom, 70 mL Frits for reservoirs 3 mL, polythylene* Frits for reservoirs 6 mL, polythylene* Reservoirs with 2 x 20 m frits, 3 mL** Reservoirs with 2 x 20 m frits, 6 mL**

Unit 50/pk 50/pk 50/pk 50/pk 100/pk 100/pk 50/pk 50/pk

Part No. 700-4005 700-4006 700-4007 700-4008 700-4030 700-4031 700-4015 700-4016
*Use two frits in each tube. **Used with high-suspended-solids samples.

Sample Preparation

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CombiZorb Scavengers CombiZorb Scavengers A Purification Tool for Combinatorial Chemistry


Less Swelling Broad Solvent Compatibility More Capacity per Unit Volume Drug discovery frequently requires compounds to be of high purity in order to properly evaluate their bioactivity and to produce pure combinatorial compound libraries. CombiZorb Scavengers allow the rapid removal of undesired reaction by-products and excess reactants. CombiZorb Scavengers have been developed to solve some of the recurrent problems associated with commercial products such as the polystyrene-divinylbenzene (PS-DVB) copolymersswelling, lack of solvent compatibility, static charge, and handling difficulties. CombiZorb Scavengers come in two varieties: CombiZorb S Silica-Based Scavengers and CombiZorb MP Macroporous Polymer-Based Scavengers. They are sold in bulk in 10 g and 100 g bottles (one kilogram bottles on request as a special quote). CombiZorb Scavengers can be packed into flow-through cartridges, 96-well plates or used in solution.

CombiZorb S Scavengers
CombiZorb S Silica-based Scavengers are synthesized from Type B high purity spherical, rigid silica gel particles with low trace metal content. Scavenger phases are covalently bonded to the base silica using organosilane-bonding chemistry. The CombiZorb S products: Show no swellingunlike conventional functionalized gel-type polystyrene/divinylbenzene resins that can swell up to 500% of their dry volume. Free-flowing, easy to handle when packing columns, 96-well plates, and when transferring the sorbent; no static charge. Are based on high purity (99.995%) porous silica microspheres with low metal content; no interfering reactions or catalysis with metals or contamination from sorbent.

Sample Preparation

CombiZorb MP Scavengers
CombiZorb MP Polymeric Scavengers were created to overcome the problems with lightly crosslinked polymer products. The CombiZorb MP products: Have significantly less swelling (maximum 50%) than standard PS/DVB resins (>500%); they show higher, more consistent capacity/volume and do not need to pre-swell. Show broad solvent compatibility and can be used with polar or non-polar solvents between reaction and scavenging steps and there is no need to switch solvents. Have high purity with no extractables to foul reaction products. Helpful Hint: If you are unsure as to the CombiZorb Scavenger product that may fit your needs, a convenient Variety Pack (P/N 550550-901) is available. This pack contains six of the most popular CombiZorb phases.

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CombiZorb Scavengers
CombiZorb Physical Data
CombiZorb Scavengers Structure Characteristics Loading Capacity Applications

CombiZorb S Scavengers

S-Triamine

Si

H N

)2 NH2

Spherical, ultra-pure silica Density: 1.3 g/mL Particle Size: 2080 m No swelling Spherical, ultra-pure silica Density: 1.3 g/mL Particle Size: 2080 m No swelling Spherical, ultra-pure silica Density: 1.3 g/mL Particle Size: 2080 m No swelling Spherical, ultra-pure silica Density: 1.3 g/mL Particle Size: 2080 m No swelling

1.21.6 meq/g

Scavenging acids, acid chlorides, acid anhydrides, chloroformates, isocyantes, aldehydes and many other electrophiles Scavenging acids, acid chlorides, acid anhydrides, chloroformates, isocyantes, aldehydes and many other electrophiles Scavenging amines and other basic compounds

S-Monoamine

Si

NH2

0.60.9 mmol/g

S-Sulfonic acid

Si

SO3H

0.40.6 mmol/g

Sample Preparation

S-Tertiary amine

Si

NMe2

0.60.9 mmol/g

Scavenging acids generated from condensation reactions and may catalyze acylation reactions

CombiZorb MP* Scavengers

MP-Aldehyde

MP

Spherical, macroporous polystyrene/DVB particle Density: 0.6 g/mL CHO Particle Size: 30100 m Swelling: 2030% in THF or CH2Cl2 Spherical, macroporous polystyrene/DVB particle Density: 0.6 g/mL Particle Size: 30100 m Swelling: 2030% in THF or CH2Cl2 Spherical, macroporous polystyrene/DVB particle Density: 0.6 g/mL Particle Size: 30100 m Swelling: 3050% in THF or CH2Cl2

1.21.8 mmol/g

Scavenging hydrazines, hydroxylamines, primary amines, organometallic reagents (organolithium and Grignard reagents), and other nucleophiles Scavenging hydrazines, hydroxylamines, primary amines, organometallic reagents (organolithium and Grignard reagents), and other nucleophiles Scavenging aldehydes and ketones and used as solid phase reagents in some catch and release applications

0.91.3 mmol/g

MP-Isocyanate

MP

NCO

1.52.5 mmol/g

MP-Sulfonyl hydrazide MP

SO2NHNH2

* MP-macroporous polystyrene/divinylbenzene

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CombiZorb Scavengers
CombiZorb Physical Data (continued)
CombiZorb Scavengers Structure Characteristics Loading Capacity Applications

CombiZorb MP Scavengers

MP-Sulfonyl chloride

MP

SO2Cl

Spherical, macroporous polystyrene/DVB particle Density: 0.6 g/mL Particle Size: 30 100 m Swelling: 30 50% in THF or CH2Cl2 Spherical, macroporous polystyrene/DVB particle Density: 0.6 g/mL Particle Size: 30 100 m Swelling: 30 50% in THF or CH2Cl2

1.6 2.0 mmol/g

Scavenging alcohols, phenols, and amines, more widely used as solid phase reagents in some catch and release applications

1.7 2.5 mmol/g

MP-Piperidinomethyl

MP

Use as catalyst for acylation reactions in place of tertiary amine and for scavenging acids generated from condensation reactions

Note: To get the complete CombiZorb Scavenger story with practical applications examples, please go to the Agilent website www.agilent.com/chem and print a copy of Publication #5968-7313E.

Sample Preparation

CombiZorb Scavenger S-Triamine* S-Monoamine* S-Sulfonic acid* S-Tertiary amine* MP-Aldehyde* MP-Isocyanate* MP-Sulfonyl hydrazide MP-Sulfonyl chloride MP-Piperidinomethyl Variety pack

Part No. 550145-138 550245-238 550145-118 550245-218 550145-124 550245-224 550145-148 550245-248 510100-150 510200-250 510100-160 510200-260 510100-134 510200-234 510100-114 510200-214 510100-170 510200-270 550550-901

Size 10 g 100 g 10 g 100 g 10 g 100 g 10 g 100 g 10 g 100 g 10 g 100 g 10 g 100 g 10 g 100 g 10 g 100 g (6) 5 g Bottles
*Included in Variety Pack

CombiZorb Scavenger

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Sample Filtration
Syringe Filters

Sample Filtration
Syringe Filters
Membrane syringe filters are recommended to clarify any samples that need further analysis or use where particulate matter in the sample solution may cause a problem. Most often they are used for small volume HPLC samples. The membrane filters are contained in an inert polymeric housing. No glue or binders are used in their construction to ensure that no extractables are present. The housing is designed to ensure that the sample is spread over the whole surface of the membrane so that maximum membrane capacity is used. Syringe filters are ready-to-use and are quite convenient. You merely attach the Luer-tipped syringe filled with the sample solution to the housing and push the sample through the pre-cleaned filters. Why Filter Your Samples for HPLC? Protects your column, particularly the newer sub-five micron microparticulate columns, capillary columns and column inlet frits, against plugging (blockage) from sample particulate matter. Extends column lifetime.

Sample Preparation

Protects your injection valve components from possible damage, scratching, and increased wear by sample particulate matter. Minimizes instrument downtime. Why Purchase Agilent Syringe Filters? We offer the most popular sizes, porosities, and membrane types at attractive pricing Both Luer and the lowest volume mini-tips available All syringe filters are pre-sterilized by gamma irradiation Low extractables for high sensitivity work High protein recovery for biological samples Regenerated cellulose filters are batch-tested under HPLC conditions New Econofilters are attractively priced in larger quantity packages How to Select the Right Membrane Syringe Filter? 1) Choose the size of filter based on the volume of sample that must be filtered. 2) Choose the porosity of the filter based on the size of potential particulates that may be present in your sample. Remember, the finer the porosity the more pressure it will take to pass sample through the filter. If you have a great deal of particulate matter, you may want to use a glass fiber filter, place a glass fiber pre-filter in front of your membrane filter to prevent rapid plugging or use a 2-in-1 filter that has a built-in prefilter in a single housing. 3) Choose the membrane type based on the solvent that you want to filter.

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Sample Filtration
Syringe Filters Filter Types
All filter inlets are female Luer-compatible, have inert polypropylene or polycarbonate housings and come in four diameters: 30 mm filters are designed for larger sample volumes or for solvent filtration. The wide cross sectional area (5.1 cm2) offers an increased filtration speed over the smaller diameter filters. The holdup volume is less than 50 L. 25 mm Econofilters are designed for high throughput laboratories that require an economical filter packaged in larger quantities at a substantial cost savings. The filters have a moderately wide cross sectional area (4.2 cm2) with a holdup volume of less than 50 L. 13 mm filters are ideal for most applications. They offer the best compromise between holdup volume and sample volume and are our most popular size. Sample volumes are typically in the 1-10 mL range and the holdup volume is less than 10 L. 3 mm filters are ideal for small sample (< one mL), where a minimum loss of sample is desirable. The membranes and housings are compatible with both organic and aqueous samples. The holdup volume is less than 7 L.

Sample Preparation

Two porosities are available: 0.45 micron pores and 0.20 micron pores. Use the 0.45 micron version to remove particles that are detrimental to most columns; the 0.20 micron filters are used to remove the smallest particles. Pre-filters have a 100% borosilicate glass fiber membrane that is chemically inert and resistant to most solvents. The high surface area of the rigid fiber structure provides outstanding particle retention capacity while maintaining low flow resistance. The GF53 will retain coarse particles down to approximately 3 m in diameter and the GF92 down to approximately 2 m in diameter. They can be used standalone or in series with a membrane filter. 2-in-1 filters are a two-layered filter in a single housing with a built-in glass fiber pre-filter on the top layer and a membrane filter on the bottom layer. The coarse pre-filter removes the larger particulates that would plug the membrane filter. This type of filter is recommended for difficult-to-filter samples. Because the pre-filter retains coarse particles, it requires less force to push liquid sample through the filter, thereby providing higher throughput. The combination costs less than two individual filters and saves time, sample and money.

Glass Fiber Prefilter Membrane Filter

Membrane Types
Agilent Technologies provides the most popular type of membranes to cover a very wide range of applications. The main selection criteria are solvent-compatibility and porosity. To assist you in making sure that the former criterion is met, the chemical resistance table lists the most popular solvents used in HPLC along with the compatible membrane types. As you can see, the regenerated cellulose and PTFE membranes have the widest range of solvent compatibility and are the preferred membranes for HPLC use. Our most popular membrane type, the regenerated cellulose membrane is priced slightly lower than the PTFE.
2-in-1 Filter

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Sample Filtration
Syringe Filters
PTFE membranes are compatible with almost all solvents, acids and bases. Nylon 66 membranes are compatible with most solvents, organic and aqueous; but use with strong acids, methylene chloride and DMF is not recommended. Cellulose nitrate is primarily used for pre-filters and is compatible with many but not all aqueous or non-aqueous solvents (see Chemical Resistance Table). Cellulose acetate membranes are not compatible with organic solvents. They are well suited for aqueous solutions and are specially recommended for proteins and protein-related samples. Regenerated cellulose membranes are recommended for general HPLC sample preparation as well as filtration of aqueous biological samples and organic solvents. The regenerated cellulose filter is close to a universal filter for HPLC. In addition to its wide range of solvent compatibility, low protein binding, and attractive pricing, it offers extremely low extractables. Extractables with uv-absorbance can be particularly troublesome to liquid chromatographers. Thus, all regenerated cellulose filters are batch-certified for water, acetonitrile and methanol extractables as determined under stringent HPLC test conditions (wavelengths of 214 and 254 nm) on an Agilent Technologies Series 1100 HPLC System. A certificate of analysis (C of A) is available for each batch of regenerated cellulose filters. In addition, we have changed the packaging and provide the regenerated cellulose in a reusable, easy-opening plastic box.

Sample Preparation

Chemical Resistance Table for Membrane Filters*


Substances Cellulose nitrate 1-Hexanol 1.4-Dioxan Acetic acid, 10% & 25% Acetone Acetonitrile Aliphatic hydrocarbons Ammonia, 1 M Aromatic hydrocarbons Benzene Boric acid Carbon tetrachloride Carboxylic acid Chloroacetic acid Chloroform Cyclohexane Cyclohexanol + + + + + + + + + + o + + o + + + + + o + o + Membrane filters Cellulose acetate Regenerated cellulose + + + + + + + + + + + + o + + + Nylon 66 + N/A + + + + N/A + o + + + PTFE + o + + + + + + + + + + o + +

*Code for Table: + = Resistance, o = Limited resistance, = Not resistant, N/A = Not available

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Sample Filtration
Syringe Filters
Chemical Resistance Table for Membrane Filters (continued)*
Substances Cellulose nitrate Diethylether Dimethyl formamide Dimethyl sulfoxide Ethanol=<98% Ethyl acetate Ethylene chloride Ethylene glycol Formic acid, 25% Hexane Hydrochloric acid, 25% i-Propanol Methanol Nitric acid, 25% Pentane Phosphoric acid, 25% Phosphoric acid, 45% Potassium hydroxide, 1 M Salt solutions, aqueous Sodium hydroxide, 1 M Tetrachloroethane Tetrahydrofuran Toluene Trichloroacetic acid, 10% Trichloroethane Trichloroethylene Xylene o o o + + + + o + + o + + + + + + + o + o + o + + + + o + + o + o o + o o + Membrane filters Cellulose acetate Regenerated cellulose + o o + + + + + + + + + + + + o o + + + + + + + Nylon 66 + + N/A + + + + + + o + o o + o + + o o o + PTFE o + + + + + + + + + + + + + + + + + + + o + + + + +

Sample Preparation

*Code for Table: + = Resistance, o = Limited resistance, = Not resistant, N/A = Not available

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Sample Filtration
Syringe Filters Recovery
Sample recovery is an important performance criterion for membrane filters. Nobody wants to spend time collecting and preparing their precious sample only to find that in the filtration step, the sample is irreversibly sorbed, affecting the analytical results or reducing the amount of sample analyte for future steps in the process.

Sample Preparation

Recovery of Polynuclear Aromatic Hydrocarbons

Low Protein Adsorption of Cellulose Acetate and Regenerated Cellulose Membranes

Pre-Filters (Stand alone) (100/pk)


Description Part No.

Pre-filter glass fiber GF53 30 mm Pre-filter glass fiber GF91 30 mm

5042-1393 5042-1370

Membrane Filters (100/pk)


Description Part No.

Filter PTFE 30/20 with Luer tip Filter PTFE 30/45 with Luer tip Filter PTFE 13/45 with Luer tip Filter PTFE 13-45 with mini tip Filter cellulose acetate 30/45 with Luer tip Filter nylon 3/45 with Luer tip Filter nylon 3/20 with Luer tip

3150-0753 3150-0754 5185-5836 5185-5837 5061-3363 5061-3367 5061-3368

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Sample Filtration
Syringe Filters
Regenerated Cellulose Membrane Filters (100/pk)
Description Part No.

Filter regenerated cellulose 30/20 with Luer tip Filter regenerated cellulose 30/45 with Luer tip Filter regenerated cellulose 13/20 with Luer tip Filter regenerated cellulose 30/45 with Luer tip Filter regenerated cellulose 13/20 with Luer tip Filter regenerated cellulose 13/45 with Luer tip

5061-3354 5061-3364 5064-8222 5064-8221 5061-3366 5061-3365

2-in-1 Filters
Description Part No.

2-in-1 Filter cellulose nitrate glass fiber 2-in-1 Filter regenerated cellulose glass fiber

5042-1391 5042-1392

Sample Preparation

Membrane Econofilters (200/pk)


Description Part No.

Econofilter regen cellulose 25/20 Econofilter regen cellulose 25/45 Econofilter nylon 25/20 Econofilter nylon 25/45 Econofilter PTFE 25/20 Econofilter PTFE 25/45

5185-5830 5185-5831 5185-5832 5185-5833 5185-5834 5185-5835

Syringes for Use with Sample Filters (10/pk)


Description Part No.

Disposable syringes 20 mL

5062-8534

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GC and GC/MS
Table of Contents
GC and GC/MS Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .149 GC Supplies Septa, Premium and General Purpose . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .149153 Merlin Microseal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .154 Inlet Liners . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .155159 Ferrules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .160163 Column Connectors/Splitters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .164166 Valves and Loops . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .167168 GC Instrument Parts and Supplies Purge and Trap Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .169172 Automatic Liquid Sampler Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .173 Headspace Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .174175 Split/Splitless Inlet Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .175176 6890 PTV Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .177 6890 Cool On-Column Inlet Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .178179 6890/6850 Packed Port Inlet Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .180 6890/6850 Detector Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .181 6890/6850 FID Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .182183 6890 NPD Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .184185 6890/6850 TCD Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .185186 6890 ECD Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .187 6890 FPD Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .187 5890 Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .188192 5890 Detector Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .192-198 GC/MS Parts and Supplies MSD Maintenance Schedule . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .199203 MSD Ion Source . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .204207 MSD Filaments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .207 MSD Vacuum System and Pumps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .208209 MSD Electron Multipliers and Replacement Horn . . . . . . . . . . . . . . . . . . . . . . . . . . .210 MSD NoVents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .211212 8270 Semi-Volatiles Application Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .212 MS Engine Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .213215 MS Test and Performance Sample . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .216 GC and GC/MS Columns . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .217323 GC/MS Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .324340 GC/MS Applications Retention Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .341347 Environmental . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .347380 Food and Flavors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .381393 Industrial Chemicals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .394414 Life Sciences . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .415424 Petroleum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .425441

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GC Supplies
Septa

Ensuring Peak Performance in Your Application


Agilent has expanded its offering of GC columns and supplies by adding not just new products, but a powerful brandJ & W Scientific. The selection listed here now presents a range that is virtually unmatched in the industry. That means you can find precisely what you need from a single supplier, whatever your application. What's more, you'll know that each product has been developed and tested to optimize your Agilent system. And the performance of every column is certified to match our published quality control specifications for reliable, repeatable results. In this section, you'll see not only listings of parts and supplies, you'll see highly useful product selection guides, compatibility charts, and chromatograms to help you make the right choices for your specific applications. To make it still more useful, we have integrated a troubleshooting and maintenance guide into this section. Now everything you need to ensure peak performance is right at your fingertips.

Septa
One of the key components of sample introduction is the inlet septum. All columns must have carrier gas head pressure to establish flow through the column. Septa maintain the leak-free seal and exclude air from the inlet. They come in many different sizes and are made from many different types of material specific to inlet type and analysis needs. Septa are usually available according to their recommended upper temperature limits. Lower temperature septa are usually softer, seal better, and can withstand more punctures (injections) than their high-temperature counterparts. If used above their recommended temperatures, however, they can leak or decompose. This causes sample losses, lower column flow, decreased column life and ghosting.

GC and GC/MS

What is the Function The septum isolates the sample flow path from the outside world. It must provide a barrier that is readily penetrated by the injector needle while maintaining internal pressure without contaminating the analysis. Septa are generally made of special high temperature, low-bleed silicone rubber formulations.

Why Replace Septa should be replaced regularly to avoid: leaks decomposition sample loss reduced column or split vent flow ghost peaks column degradation

How to Minimize Problems Avoid problems by: using with the recommended temperature range changing regularly installing the retainer nut hand tight using septum purge when available using autoinjectors using sharp syringe needles

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GC Supplies
Septa Septa Troubleshooting
Symptom Extra Peaks/Humps Possible Causes Septum bleed. Remedy Turn off injector heater. If extra peaks disappear, use septum specified for higher temperature or analyze at lower inlet temperature.

Baseline Change After Large Peak

Large leak at septum Replace septum and use during injection and smaller diameter needles. for a short time thereafter (common with large diameter needles).

GC and GC/MS

Retention Times Prolonged

Carrier gas leaks at Check for leaks. Replace septum or column septum or tighten connection. connections if necessary.

Summary of Premium Inlet Septum Characteristics


Septum Type BTO (Bleed and Temperature Optimized) Long Life Advanced Green Bleed


Lifetime


Temperature Limits to 400C

(optimized for high temperature)


   

to 350C to 350C

= best = very good = good

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GC Supplies
Premium Septa Premium Septa
Premium CenterGuide Septa for Improved Performance
Our premium septa have a recess on the injection side, to guide the syringe needle to the same point with every injection. Center point guides the needle for easy penetration and less coring Reduces needle bending Precision molding assures accurate fit in the inlet Each batch tested on an Agilent 6890 GC-FID for bleed

Bleed and Temperature Optimized Septa (BTO)


Extended temperature range, low-bleed Maximum injection port temperature 400C Virtually eliminates injection-port sticking Pre-conditioned; packaged in glass to prevent contamination Ideal for use with low-bleed, Mass Spec capillary columns
P/N 5183-4757

Bleed and Temperature Optimized Septa


Description 11 mm septa for 4890, 5890, 6850 and 6890 GCs 5 mm septa through-hole for on-column Unit 50/pk 50/pk Part No. 5183-4757 5183-4758

GC and GC/MS

Long-Life Septa
Pre-pierced for extended life and reduced coring The preferred septum for autosamplers Ideal for overnight runs Up to 400 injections per septum Maximum injection port temperature 350C Soft, 45 Durometer, easy on autosampler needles

P/N 5183-4761

Long-Life Septa
Description 11 mm septa for 4890, 5890, 6850 and 6890 GCs 5 mm septa through-hole for on-column Unit 50/pk 50/pk Part No. 5183-4761 5183-4762

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GC Supplies
Premium/General Purpose Septa
Advanced Green Septa
True long-life, high temperature green septum More injections per septum Reduced injection port sticking Maximum injection port temperature 350C Packaged in glass vials for high purity Economical alternative to competitors Green septa

P/N 5183-4759

Agilent Advanced Green Septa


Description 11 mm septa for 4890, 5890, 6850 and 6890 GCs 5 mm septa through-hole for on-column Unit 50/pk 50/pk Part No. 5183-4759 5183-4760

Bleed Test - Comparison of GC Septa


GC conditions for all chromatograms Instrument: Agilent 6890

GC and GC/MS

Column: P/N: Carrier: Oven:

Injector: Detector:

DB-5 15 m x 0.53 mm id, 1.5 pm film 125-5012 Helium at 4 mL/min constant flow 50C for 15 min 50-250 at 20C/min 250C for 15 min Splitless, 250C 10 minute purge activation time FID, 280C

General Purpose Septa


Agilents General Purpose Septa are made from an enhanced injection-molded silicone rubber material. The septa material, dark red or gray in color, is specified to withstand over 200 automatic injections at an injection port temperature of 350C. You can have confidence in your chromatographic results knowing that each lot of septa is placed through a demanding QC test to ensure that only the highest quality product is delivered to your laboratory.

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GC Supplies
General Purpose Septa
General Purpose Septa are a cost effective choice which provide
Low bleed for reduced instrument maintenance downtime and increased laboratory productivity Less frequent replacement for long life time and the ability to withstand more than 200 injections at maximum injection port temperatures Easy penetration and the durability to remain resistant to coring and leaking from multiple injections Packaged in airtight jars to minimize the risk of contamination and the need for septa reconditioning

General Purpose
Description Low-Bleed Gray Septa 11 mm for 5880, 5890, 6850 and 6890 GCs 11 mm for 5880, 5890, 6850 and 6890 GCs 9.5 mm (3/8 in.) for 5700 series and 5830/40 GCs 9.5 mm (3/8 in.) for 5700 series and 5830/40 GCs Low-Bleed Red Septa 11 mm solid for 5880, 5890, 6850 and 6890 GCs 11 mm with partial through-hole for 5880, 5890, 6850 and 6890 GCs 5 mm through-hole for on-column inlets, automatic or manual injections 5 mm solid for high column backpressure, on-column inlets 9.5 mm (3/8 in.) for 5700 series and 5830/40 GCs Blue Septa 11 mm for 5880, 5890, 6850 and 6890 series GCs White Septa 9.5 mm (3/8 in.) Teflon backed for 5700 series and 5830/40 GCs 9.5 mm (3/8 in.) Teflon backed for 5700 series and 5830/40 GCs 12/pk 100/pk 5080-8745 5080-8707 5/pk 9301-1083 25/pk 25/pk 25/pk 25/pk 24/pk 5181-1263 5181-3383 24/pk 144/pk 24/pk 144/pk 5080-8896 5080-8894 5080-8728 5080-8726 Unit Part No.

GC and GC/MS

5181-1260 5181-1261 5181-1283

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GC Supplies
Merlin Microseal The Merlin Microseal
Low bleed, longer life alternative to standard septa for split/splitless injection Has a lifetime of more than 2000 injections, depending on samples and operating conditions Greatly reduced instrument downtime for septa changes and injection port liner changes due to septa particulates Two distinct sealing mechanisms: Double O-ring type seal around the syringe needle Spring assisted duckbill to seal the injection port

Merlin Microseal
Description High Pressure High pressure Merlin Microseal starter kit (microseal septa and nut) Microseal high pressure septum* Microseal high pressure nut High sample volume septum kit contains: Merlin high pressure Microseal, six 23-gauge syringes, 500 vials and caps Standard Pressure Microseal septum, SS, rubber (30 psi) Microseal PTFE nut liners Merlin Microseal Kit original low pressure system includes nut and septum Merlin Microseal Kit original low pressure system includes nut and 2 septum 2/pk 5182-3442 5182-3444 5182-3445 5181-8839 Unit Part No.

GC and GC/MS

5181-8815 5182-0853 5181-8816 5181-8833


*Not interchangeable with older version.

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GC Supplies
Inlet Liners

Inlet Liners
Agilent offers a complete selection of liners for your Agilent GCs inlet. These liners are designed and engineered to meet strict Agilent specifications for dimensional accuracy and inertness towards demanding compounds. For example, the biggest difference between Agilent splitless liners versus competitor designs is the OD of our splitless liners are ground and polished to exacting dimensional tolerances. This ensures a perfect fit into our inlets and optimum splitless injection performance. Choosing the proper liner for a specific application can be a difficult and challenging task. The three liner characteristics that must be considered for each application are: Liner volume Liner treatments or deactivation Any liner design features that might affect carrier gas flow through the inlet or sample vaporization What is the Function Liners are the centerpiece of the inlet system in which the sample is vaporized and brought into the gas phase. Why Replace These problems will occur if the liner is not changed on a regular basis or if the correct liner is not used: peak shape degradation solute discrimination poor reproducibility sample decomposition ghost peaks How to Minimize Problems Change liners on a regular basis determined by: previous use pattern sample cleanliness chromatographic abnormalities such as: peak shape changes peak discrimination poor reproducibility sample pyrolysis

GC and GC/MS

Liner Troubleshooting
Symptom Tailing Peaks Possible Causes Sample components adsorbed by column, inlet liner or contaminated gold inlet seal. Remedy Use new, deactivated liner or clean old liner and replace glass wool.

Needle hitting and breaking packing in inlet liner. Partially remove packing from liner or use without packing. Column end poorly cut (sample absorption). Normal Problem Remove column. Make a clean, square cut using a reliable capillary fused silica cutting tool (such as a ceramic wafer or the Agilent Column Cutter), then reinstall column. Make sure total flow in inlet is above 40 mL/min. Remove inlet liner and check cleanliness. Use new, deactivated liner or replace glass wool and packing.

Broken or chipped inlet liner. Leading Peaks Sample decomposing.

Normal

Problem

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GC Supplies
Inlet Liners
Symptom Baseline Rise Before or After Peak Possible Causes Remedy

Sample decomposing. Remove inlet liner and check cleanliness. Use new, deactivated liner or replace glass wool and packing.

Normal

Problem

Problem Column and inlet liner misaligned. Check installation of column end and inlet liner, adjust if necessary.

Baseline Change After Large Peak

Normal Unresolved Peaks

Problem

Problem Column or inlet liner Use a guard column to contaminated or prolong column life. column deteriorating. Remove inlet liner and check cleanliness. Use new, deactivated liner or place or replace glass wool and packing. Trim the front end of the column a minimum of 6 inches.

GC and GC/MS

Normal

Problem

Problem

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GC Supplies
Inlet Liners Inlet Liners
Liner Volume Description
Split/Splitless Inlet Liners

Unit 5/pk 25/pk

(L) 870

1/pk 5183-4711

Liner, general purpose split/splitless, glass wool, taper, deactivated


Splitless Inlet Liners

5183-4712 5183-4713

Liner, splitless, single-taper, no glass wool, deactivated Liner, splitless, single-taper, glass wool, deactivated Liner, splitless, double-taper, no glass wool, deactivated
Split Inlet Liners

900

5181-3316 5183-4695 5183-4696

900

5062-3587 5183-4693 5183-4694

800

5181-3315 5183-4705 5183-4706

Liner, split, low press. drop, glass wool, taper, deactivated

870

5183-4647 5183-4701

GC and GC/MS

5183-4702 Split Inlet Liner 990 Liner, split, glass wool, non-deactivated Split Inlet Liners for Manual Injection Liner, split, with cup, no glass wool Liner, split, with cup, glass wool, and packing (not recommended for use with electronic pressure control [EPC]), for manual injection
Direct Inlet Liners

19251-60540 5183-4691 5183-4692 18740-80190 5183-4699 5183-4700

800

800

18740-60840 5183-4697 5183-4698

Liner, direct, 2 mm id, deactivated

250

5181-8818 5183-4703 5183-4704

Liner, direct, 2 mm id, non-deactivated, quartz Liner, direct, 1.5 mm id, nondeactivated (use for gas samples, headspace, or purge and trap applications) Liner, straight, splitless 4.0 mm id

250

18740-80220 5183-4707 5183-4708

140

18740-80200 5183-4709 5183-4710

990

210-3003 210-3003-5

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GC Supplies
Inlet Liners
Direct Connect Liners
Press fit connection to the column No sample exposure to the inlet Included in 8270 EPA application kit Optimized hole size and placement
Description Single taper direct connect liner, splitless 4 mm id, deactivated Dual taper direct connect liner, splitless 4 mm id, deactivated Part No. G1544-80730 G1544-80700

Focus Liners
Improved reproducibility Precise amount of glass wool in the ideal position traps non-volatile sample residue Glass wool wipes any residual sample from the needle
Description Unit Part No.

Focus liner, tapered, 4.0 mm, glass wool, 6.3 mm x 78.5 mm Focus liner, tapered, 4.0 mm, glass wool, 6.3 mm x 78.5 mm

5/pk 5/pk

210-4004-5 210-4022-5

Megaliners

GC and GC/MS

Directly inject samples into 0.45 or 0.53 mm ID columns Column seals to the base of the liner reducing solvent tailing Virtually all of the sample transfers to the column
Description Part No.

Megaliner 22 gauge, 4.0 mm, no glass wool, 6.3 x 78.5 mm Megaliner 26 gauge (T-top), 4.0 mm, no glass wool, 6.3 x 78.5 mm

210-2063H22 210-2063H26

Liner O-Rings
Liners are sealed in the inlet with O-rings or graphite seals Fluorocarbon O-ring seals are easier to replace than graphite that deforms and flakes Graphite seals are used when inlet temperatures exceed 350C
Description Unit Part No.

Fluorocarbon O-ring Graphite O-ring for splitless liner Graphite O-ring for split liner

12/pk 10/pk 10/pk

5180-4182 5180-4173 5180-4168

P/N 5180-4182

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GC Supplies
Inlet Liners Megabore Injection Port Liners for J&W Injection Port Conversion Kits
Direct Injection Liners
Minimizes the potential for sample backflash Most efficient transfer of the sample from the inlet to the column

Hot On-Column Liners


Inject samples directly into the column in a standard 26 gauge syringe

Flash Vaporization Megabore Liner


Can be packed in glass wool to minimize the amount of non-volatile residues reaching the column

Liners
Description Direct Injection Liner 1/4 in. Port Direct Injection Liner - 5890 Direct Injection Liner 1/4 in. Port Direct Injection Liner - 5890 Hot On-column Liner 1/4 in. Port Hot On-column Liner - 5890 Silanized Flash Vaporization Megabore Liner Length (mm) 220.0 85.0 220.0 85.0 220.0 85.0 220.0 Part No. 210-1064 210-1064-G 210-1063* 210-1063-G* 210-1066 210-1066-G 210-1070**
Hot On-column Direct Injection

GC and GC/MS

*These liners are not precut to fit specific injectors. They must be cut to the proper length before use. **This liner has no top restriction; it can be packed and cleaned easily.

Liner Ferrules for J&W Injection Port Conversion Kits


Description Graphite/Vespel Ferrule 6 mm, 1/4 in. Graphite Ferrule for 6 mm Mega/Split-Splitless Injection Port Liner, Double Taper Unit 10/pk 10/pk Part No. 500-1060 500-2066

Note: These liners are designed to fit J&W MegaBore conversion kits only and are not direct replacements for Agilent part numbers.

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GC Supplies
Ferrules

Ferrules
Using the wrong ferrule or a worn out ferrule to seal your column connection can result in inconsistent and unreliable chromatography. An improper ferrule can cause leaks which allow air and other contaminants to enter the instrument through the column seal, causing major interference with column and detector performance. For optimum performance, ferrules should be replaced every time the column is replaced and when performing column maintenance. At Agilent, we offer a comprehensive selection of ferrules made of different materials and configurations for a PerfectFit with your instrument and a leak-free connection between the column and injector. Three main types of ferrules are used with capillary GC columns: graphite, Vespel and Vespelgraphite composites. Graphite ferrules can withstand temperatures as high as 450C, and Vespel and Vespel-graphite ferrules are rated to 280C and 350C respectively.

What is the Function Ferrules seal the connection of the column or liner to the system. The ideal ferrule provides a leak-free seal, accommodates various column outer diameters, seals with minimum torque, will not stick to the column or fittings, and will tolerate temperature cycling.

Why Replace

How to Minimize Problems General technique for installing ferrules:

GC and GC/MS

Signals that a ferrule is damaged include: background noise from oxygen diffusing into the system column bleed catalyzed by oxygen sample degradation loss of sample increase in detector signal/noise poor retention time reproducibility

dont overtightenfingertighten column nut, then use wrench to tighten a 1/4 turn maintain cleanliness bake out ferrules prior to use avoid contaminationfingerprints, oils inspect used ferrules with magnifier for cracks, chips, or other damage before reusing them change ferrules when new columns or injector/detector parts are installed

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GC Supplies
Ferrules Ferrule Troubleshooting
Problems possible after changing ferrules

Normal Peaks Correct column positioning in both injection port and FID

Tailing Solvent Peaks Column positioned incorrectly in the injection port or possible ferrule particle in the carrier gas flow path

Wrong Peak Ratios Column positioned in the inlet (either too far or not far enough; verify 4-6 installation distance)

Ferrule Selection Recommendations


Ferrule/ Seal Type Upper Temperature Limit Usages Advantages Limitations

Graphite (100%)

450C

General purpose for capillary columns. Suitable for FID and NPD. Recommended for high temperature and cool on-column applications. General purpose for capillary columns. Recommended for MS or oxygen sensitive detectors. Most reliable leak-free connection. Isothermal operation. Can be reused or removed easily Excellent sealing material when making metal or glass connections.

Easy to use stable seal Higher temperature limit Can be removed easily Mechanically robust Long lifetime

Not for MS or oxygen sensitive detectors. Soft, easily deformed or destroyed Possible system contamination

GC and GC/MS

Vespel/Graphite 350C (85%/15%)

Not reusable Flows at elevated temperature Must retighten frequently Leaks after temperature cycle Flows at elevated temperature Must retighten frequently

Vespel (100%)

280C

Mechanically robust Long lifetime Can be reused or removed easily

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GC Supplies
Capillary Column Ferrules Capillary Column Ferrules
Ferrule ID (mm) Column ID (mm) Unit Part No. General Purpose Graphite Ferrules (Short Ferrules)

0.5 1.0 0.4 0.8

0.1, 0.2, 0.25, 0.32 0.53 0.05-0.25 0.45, 0.53

10/pk 10/pk 10/pk 10/pk

5080-8853 5080-8773 500-2114 500-2118

85% Vespel, 15% Graphite Ferrules (Short Ferrules)

0.4 0.5 0.8

0.1, 0.2, 0.25 0.32 0.45, 0.53

10/pk 10/pk 10/pk

5181-3323 5062-3514 5062-3512

Preconditioned 85% Vespel, 15% Graphite Ferrules (Long Ferrules)*

0.3 0.4 0.5 0.8

0.1 0.1, 0.2, 0.25 0.32 0.53

10/pk 10/pk 10/pk 10/pk

5062-3507 5062-3508 5062-3506 5062-3538

100% Vespel High-Performance Ferrules (Short Ferrules)**

GC and GC/MS

0.4 0.5 0.8

0.1, 0.2, 0.25 0.32 0.45, 0.53

10/pk 10/pk 10/pk

5181-3322 5062-3513 5062-3511

Specialty Ferrules, 85% Vespel, 15% Graphite Two Hole

0.4 id holes 0.5 id holes


No Hole

0.1, 0.2, 0.25 0.32

10/pk 10/pk 10/pk

5062-3580 5062-3581 5181-3308

*These ferrules are recommended for use with GC/MS **These ferrules are recommended for use in isothermal analysis only.

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GC Supplies
Column Nuts/Ferrules
Column Nuts
Description Short Nuts Universal column nut, 1/16 in. hex Finger-tight column nut for 0.53 mm columns* Finger-tight column nut for 0.32 mm columns and smaller* Blanking plug, finger-tight style 6850 column nut
Long Nuts

Unit 2/pk

Part No. 5181-8830 5020-8293 5020-8292 5020-8294

2/pk

5183-4732

MS interface column nut Column nut for GC/MS ferrules


Column Wrench

05988-20066 05921-21170

Column nut wrench, 1/4 in. and 5/16 in.

8710-0510
*For use with graphite ferrules only.

Helpful Hint: Always match short nuts with short ferrules and long nuts with long ferrules for a PerfectFit.

Straight Ferrules
Description 1/4 in. Teflon 1/4 in. graphite 1/8 in. graphite 1/8 in. 15% graphite/85% Vespel 1/16 in. Teflon 1/16 in. graphite 1/16 in. VG-2 Vespel, 40% graphite 6.4 mm Vespel 1/4 in. 15% graphite/85% Vespel 10/pk Unit 10/pk 10/pk 10/pk 10/pk 10/pk 10/pk 10/pk Part No. 0100-1378 0100-1324 0100-1325 0100-1332 0100-1375 0100-1326 0100-1379 0100-1104 0100-1331

GC and GC/MS

Reducing Ferrules
Description 1/8 to 1/16 in Vespel 1/8 to 1/16 in. VG-1 Vespel, 15% graphite 1/16 in. to 0.4 mm VG-2 Vespel, 40% graphite Unit 10/pk 10/pk 10/pk Part No. 0100-1342 0100-1344 0100-1381

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163

GC Supplies
Column Union Supplies/Column Connectors
Column Union Supplies
Description Column ID Unit Part No.

Self-compensating capillary nut assembly* Split/splitless seal disk, gold plated stainless steel Fused silica column union assembly Quartz liner replacement for fused silica union Ferrule, reusable, Vespel Ferrule, reusable, Vespel Ferrule, reusable, Vespel 200, 250 m 320 m 530 m 10/pk 10/pk 10/pk

19258-20920 5182-9652 5182-0744 5182-0743 5182-0740 5182-0741 5182-0742

*The split/splitless seal disk (P/N 5182-9652) must be used with this assembly. Use Vespel ferrules only.

Column Connectors/Splitters
GC and GC/MS
Glass Capillary Column Connectors
Description
Glass Press-Fit Connector for:

Unit 10/pk 10/pk 10/pk 10/pk 10/pk 10/pk 10/pk 10/pk 5/pk 10/pk 10/pk 10/pk 5/pk 10/pk 25/pk 5/pk 25/pk

Part No. 5041-2178 5062-3553 5062-3552 5041-2176 5041-2175 5041-2173 5062-3556 5062-3554 5062-3550 5041-2174 5041-2172 5062-3555 5062-3551 5041-2171 705-0825 705-0905 705-0925 705-0903 500-1200

0.1 to 0.1 mm tubing 0.1 to 0.25 mm tubing 0.1 to 0.32 mm tubing 0.2 to 0.2 mm tubing 0.2 to 0.32 mm tubing 0.2 to 0.53 mm tubing 0.25 to 0.25 mm tubing 0.32 to 0.25 mm tubing 0.32 to 0.25 mm tubing 0.32 to 0.32 mm tubing 0.32 to 0.53 mm tubing 0.53 to 0.25 mm tubing
Glass Press-Fit Connectors

0.53 to 0.25 mm tubing 0.53 to 0.53 mm tubing Glass Union, universal 2-way Fused Silica Union, universal 2-way Fused Silica Union, universal 2-way Fused Silica Union, universal 3-way Polyimide sealing resin (5 grams)

164

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GC Supplies
Column Connectors/Splitters
Glass Capillary Column Splitters
Description
Glass Press-Fit Splitter for:

Part No. 5021-1860 5062-3549 5041-2179 5021-1861

200 m columns 250 m columns 320 m columns 530 m columns


Glass Connector Trial Kit for Capillary Columns:

Kit contains 3 ea of 530 to 530 m, 530 to 320 m, 530 to 200 m, 200 to 320 m, 200 to 200 m

5062-3509
Quartz Column Connector P/N 5181-3395

Quartz Capillary Column Connectors/Splitters


Description Quartz column connector, 0.1 to 0.53 mm Quartz deactivated column connector Quartz splitter Quartz deactivated splitter Unit 5/pk 5/pk Part No. 5181-3395 5181-3396 5181-3397 5181-3398

GC and GC/MS

Mechanical Capillary Column Connectors


Description Connector, body and nut
Vespel Ferrules, for connecting:

Quartz Deactivated Splitter P/N 5181-3398

Unit

Part No. 5061-5801

0.2 to 0.53 mm id tubing 0.32 to 0.32 mm id tubing 0.32 to 0.53 mm id tubing Fused silica, undeactivated 530 m tubing, 10 m

2/pk 2/pk 2/pk

5061-5804 5061-5805 5061-5806 160-2530-10


Connector for Agilent Split/Splitless Injection Ports P/N 5061-5801

Graphpak Capillary Column Connectors


Column ID (mm) 0.32/0.25 0.53 Connector ID (mm) 0.4 0.7 Part No. 5021-7166 5021-7164
Graphpak Connector for Agilent Capillary Detector

Graphpak Connector for Agilent Capillary Detector Ports*

Graphpak Divider for Simultaneous Sampling to Two Capillary Columns

0.32/0.25 0.53

0.4 0.7

5021-7148 5021-7146

*Order ferrules in addition to connector to fit your column. Ferrules must be ordered separately.

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165

GC Supplies
Column Connectors/Splitters
Graphpak Capillary Column Connectors (continued)
Column ID (mm) 0.2 0.32/0.25 0.53 Plug ferrule
Graphpak Divider for Simultaneous Sampling

Connector ID (mm) Unit 0.3 0.4 0.7 10/pk 10/pk 10/pk 10/pk 5/pk

Part No. 5021-7136 5021-7137 5021-7134 5021-7133 5062-3525

Ferrules for Graphpak Connection Systems

Replacement Graphpak column nut

Graphpak Connector for Agilent Capillary Injection Ports

Column ID (mm) 0.2 0.32/0.25 0.53

ID (mm) 0.3 0.4 0.7

Part No. 5021-7169 5021-7170 5021-7168

*Order ferrules in addition to connector to fit your column. Ferrules must be ordered separately.

Injector Transfer Line Splitter Union

Capillary Column Splitters


Description
Fixed inlet splitter

Tubing ID (mm) 0.22 mm 0.32 mm 0.53 mm

Part No. 5181-3390 5181-3391 5181-3392

GC and GC/MS

Column 1

Column 2

Fixed Inlet Splitter

Fixed outlet splitter (not for use with 0.53 mm)

1:1 ratio splitter


Detector 1 Detector 2

0.22 mm to 0.22 mm 0.22 mm to 0.15 mm 0.21 mm to 0.11 mm

0101-0594 5181-3389 0101-0595

1:5 ratio splitter 1:10 ratio splitter

Two-hole replacement ferrules (10/pk)


Transfer Lines

0.3 mm 0.4 mm 0.5 mm

5181-3388 5062-3580 5062-3581

Splitter Union

Column

Inlet

Variable outlet splitter for capillary columns

Fixed Outlet Splitter

Outlet splitter system includes metal body and 5 m 0.22 mm id transfer line with 0.4 mm 2-hole ferrule.

5181-3393

Variable Outlet Splitter P/N 5181-3393

166

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GC Supplies
Valves and Loops

Valves and Loops


Gas Sampling General-Purpose Valves Compatible with the 5890/6890
Description 6-port replacement valve WE series, 400 psi, 225C 6-port replacement valve WE series, Hastelloy C, 400 psi, 225C 10-port replacement valve WE series, 400 psi, 225C 6-port replacement valve WT series, 300 psi, 350C 10-port replacement valve WT series, 300 psi, 350C Part No. 5062-9508 5062-9509 5062-9510 0101-0584 0101-0585
General Purpose Gas Sampling Valves

10-port replacement valve WE series, Hastelloy C, 400 psi, 225C 5062-9511

Liquid Sampling General-Purpose Valves


Description 0.2 L replacement valve UWP series, 1,000 psi, 75C 0.5 L replacement valve UWP series, 1,000 psi, 75C 1.0 L replacement valve UWP series, 1,000 psi, 75C 0.5 L replacement valve UW series, 5,000 psi, 75C Part No. 0101-0636 0101-0637 0101-0638 0101-0639

GC and GC/MS

Parts for Interfacing Capillary Columns to W Series Valves


Description SS bulkhead ZDV union (for interfacing 530 m columns with 1/16 in. transfer line) SS bulkhead ZDV union (for interfacing 320 m columns with 1/16 in. transfer line) Polyimide 1/16 in. ferrule Polyimide liner for 530 m columns Polyimide liner for 320 m columns 1/16 in. SS counterbored nut for use with above liners Installation tool for liners Part No. 0100-1515 0100-1527 0100-1512 0100-1513 0100-1514 0100-1511 18900-20850

General Purpose Liquid Sampling Valves

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167

GC Supplies
Valves and Loops
Valve-Related Parts
Description 1/16 in. SS nut (10/pk) 1/16 in. SS ferrule (10/pk) Metering valve (for LSVs as a sample-out restrictor or as a flow-balancer for 10-100 mL/min) Air actuator Solenoid valve for controlling actuator Solenoid valve for controlling actuator (for GCs after serial number 3223A43573) Straight metering valve, 1/16 in. SS Angle metering valve, 1/16 in. SS Part No. 5181-1291 5181-1292 0101-0355

Micrometering valve (for flow balancing gas flows of 2-50 mL/min) 0101-0633 19325-60660 05890-61090 05890-61095 0101-0355 0101-0403

GC Loops Compatible with Agilent 5890/6890 (Includes Loop, Nut and Ferrule, 1/16 in.)
Description 0.25 cc 0.50 cc 1.00 cc 2.00 cc 5.00 cc 10.00 cc A. Tube, 1/16 in. SS, 280 mm long Tube, 1/16 in. SS, 400 mm long Tube, 1/16 in. SS, 375 mm long Tube, 1/16 in. SS, 560 mm long B. Tube, 1/16 in. with 1/8 in. flare, SS, 360 mm long Tube, 1/16 in. with 1/8 in. flare, SS, 520 mm long C. Tube, 1/16 in. with 1/8 in. bulkhead fitting, 520 mm long Part No. 0101-0303 0101-0282 0101-0299 0101-0300 0101-0301 0101-0302 18900-20250 18900-20280 18900-20281 18900-20300 1530-2163 1530-2167 07675-80050

GC and GC/MS

Tube, nickel, 1/16 in. with 1/8 in. bulkhead fitting, 460 mm long 18900-80255

Replacement Rotors for Gas Sampling Valves with External Loops


A B C

Description 6-port replacement rotor WE series, 400 psi, 225C 10-port replacement rotor WE series, 400 psi, 225C 6-port replacement rotor WT series, 300 psi, 350C 10-port replacement rotor WT series, 300 psi, 350C

Part No. 5181-7459 5181-7460 1535-4952 1535-4954

168

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GC Instrument Parts and Supplies


Purge and Trap Supplies

GC Instrument Parts And Supplies


When it comes to instrument replacement parts for your Agilent GCs, dont settle for an imitation, use genuine Agilent parts. Our inlets are designed, tested and manufactured by Agilent engineers to work perfectly with our instruments and give you superior performance. Plus we supply every replacement part you would ever need for your entire system, not just a few select parts. So keep your instrument operating at its very best with Agilent consumables and replacement partsthe PerfectFit for optimum performance.

Tekmar 3100 and Agilent 7695A Purge and Trap Supplies


Glassware
Fritless Sparger: Compared to a frit sparger, the fritless sparger may be the better choice when a water sample has a tendency to foam. This sparger is not appropriate for soil samples, which tend to clog the capillary tube. Available in 1/2 and 3/4 in. mount sizes. Disposable (Needle) Tubes: The most practical choice for soil (and other solid or semisolid) samples, these tubes permit easy sample loading and inexpensive disposal. Available only in 3/4 in. mount size, a 3/4 in. tube can be used on the 7695A Purge and Trap concentrator with the adapter (to convert from 7695A Purge and Trap 1/2 in., to this 3/4 in. size). Needle Sparger with Foam Trap: This sparger is designed for samples that foam, particularly if the foaming cannot be controlled by a fritless sparger. Available only in 1/2 in. mount size. Frit Sparger: This is the most efficient design in terms of purging and it is the standard choice for clean drinking water. Available in 1/2 in. and 3/4 in. mount sizes. Note: The 7695A Purge and Trap concentrator has a 1/2 in. mount, and the automatic sampler has a 3/4 in. mount. An adapter is available to mount 3/4 in. glassware on the 7695A Purge and Trap concentrator, but there is no adapter for using 1/2 in. glass on the automatic sampler.
7695 Purge and Trap

GC and GC/MS

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169

GC Instrument Parts and Supplies


Purge and Trap Supplies
Agilent 7695A and Tekmar 3100 Purge and Trap Supplies
Description
Glassware Supplies 1/2 in. Mount

Part No. 5182-0852 5182-0846 5182-0851 5182-0845 5182-0850 5182-0844 5182-0849 5182-0796 5182-0848 5182-0795 5182-0847 5182-0794

5 mL frit sparger (glassware only) 5 mL frit sparger kit with fittings 25 mL frit sparger (glassware only) 25 mL frit sparger kit with fittings 5 mL fritless sparger (glassware only) 5 mL fritless sparger kit with fittings 25 mL fritless sparger (glassware only) 25 mL fritless sparger kit with fittings 5 mL needle sparger (glassware only) 5 mL needle sparger kit 25 mL needle sparger (glassware only) 25 mL needle sparger kit
Glassware and Corresponding Needles 3/4 in. Mount

5 mL frit sparger Needle, 8 in.

5182-0893 14-3195-053TMR 5182-0894 5182-0789 5182-0895 5182-0789 12-0507-024TMR 14-5186-153TMR

GC and GC/MS

25 mL frit sparger Needle, 9 1/8 in. 5 mL fritless sparger Needle, 9 1/8 in. 150 mm disposable needle tube sparger Needle, pointed 8 in.
Sample Handling Supplies

Sample valve, 3-port assembly


Miscellaneous Supplies

5182-0793 5182-0792 14-5681-102 5182-0789 5182-0788 5182-0746 9301-1185

Female luer connector for sample valve Purge line assembly Sample needle, for 25 mL frit or fritless sparger Needle for 5 mL frit or fritless sparger Conversion kit adapter for use with 3/4 in. tube on 1/2 in. mount G1900A concentrators 5 mL sample syringe with luer connector

170

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GC Instrument Parts and Supplies


Purge and Trap Supplies

Tekmar 3100 and Agilent 7695A Purge and Trap Autosampler Supplies
Glassware Assembly (3/4 in.)
Item Description 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Sample drain line assembly Sample valve assembly, 3 port Female luer connector for sample valve Sample needle ferrule Sample needle nut Nut Ferrule Union 3/4 in. ferrules 3/4 in. nut Glassware Sample purge line top Sample purge line bottom 1/4 in. to 1/16 in. union Ferrules Nut Part No. 14-3137-200TMR 14-0036-050TMR 14-0216-016TMR 14-3123-016TMR 14-3124-016TMR 14-2087-016TMR 14-3979-016TMR 14-3914-016TMR 14-0739-016TMR 14-0857-016TMR (see table below) 14-3197-100TMR 14-3198-100TMR

1 2 3 4 5 6 7 12 8 13 9 14 10 15

11 11

GC and GC/MS

14-2261-016TMR 14-3965-016TMR 14-2087-016TMR

7695A and Tekmar 3100 Purge and Trap Autosampler Traps


Description Trap, Tenax Trap, Tenax/silica gel/charcoal Trap, Tenax/charcoal Trap, OV-1/Tenax/silica gel/charcoal Trap, OV-1/Tenax/silica gel Trap, Vocarb 3000 Trap, Bocarb 4000 Trap, BTEX Heater assembly trap Hydrocarbon trap assembly Part No. 5182-0783 5182-0781 5182-0780 5182-0779 5182-0778 5182-0775 5182-0774 5182-0773 5182-0772 5182-0771

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171

GC Instrument Parts and Supplies


Purge and Trap Supplies
Tekmar AQUATek 70 Purge and Trap Autosampler Supplies
Description
Sample Loops

Unit

Part No.

5 mL sample loop, AQUATek 70 25 mL sample loop, AQUATek 70 20 mL sample loop, AQUATek 70


Glassware for 3000/2000 Agilent 7695 (glassware only)

5182-9689 5183-4733 5183-4734

Sparger, 25 mL frit Sparger, 5 mL frit Sparger, 5 mL fritless Sparger, 25 mL fritless


Vials

5183-4735 5183-4736 5183-4737 5183-4738

Screw caps for 40 mL vials

24/pk

5183-4744

Miscellaneous Purge and Trap Supplies


Description Spare/Alternative transfer line tubing, passivated nickel, for 0.53 mm id fused silica or packed columns, 72 in. long (tubing only) Chemical Standard, Method 501 1 mL ampoule (0.2 mg in MeOH) dichlorobromomethane, dibromochloromethane
Interface Cables

Part No. 5181-8822

GC and GC/MS

8500-5901

Agilent 5890 to 7695 and TMR-3100 P&T (P/N G1900A-010) Agilent 5890/MSD to 7695 and TMR-3100 P&T (P/N G1900A-011)

5183-4745 5183-4746

172

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GC Instrument Parts and Supplies


Automatic Liquid Sampler Supplies

Automatic Liquid Sampler Supplies


Automatic Liquid Sampler Supplies
Description
Miscellaneous Autosampler Supplies

Unit

Part No.

4 mL wash vial with screw caps Diffusion caps for 4 mL vials Septa for 4 mL vial 4 mL wash vial with fill marking, caps Screw for mounting syringe Quadrant kit (4 tray sections) 7673 Basic Supply Kit: contains: 10 mL syringes (6/ea), 23/26 gauge needles, 4 mL vials with diffusion caps (144/pk), 2 mL automatic sampler vials with screw caps (1,000/pk) GC septa (25/pk), Vial racks (5/pk)

144/pk 12/pk 144/pk 25/pk

9301-0723 07673-40180 9301-1031 5182-0551 07673-20570 18596-40015 07673-60840

Note: For crimping and decapping tools, see page 37.

GC and GC/MS

18587A Bar Code Reader Labels


Description
Labels numbered (1,000/roll)

Part No.

1 to 1,000 1,001 to 2,000 2,001 to 3,000 3,001 to 4,000 4,001 to 5,000 5,001 to 6,000 Label applicator

5958-9450 5958-9441 5958-9442 5958-9443 5958-9444 5958-9445 18587-60560


Bar Code Reader Labels

Helpful Hint: For a complete selection of Vials and Caps, see the General Chromatography section of this catalog.

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173

GC Instrument Parts and Supplies


Headspace Supplies

19395A and 7694 Headspace Analysis Supplies


Agilent 19395A Miscellaneous
Description Silicone oil (oil bath capacity = 3.5 pt), 1 pt Thermosetting tape Headspace evaluation sample: 2% t-butyldisulfide, 10% nonane, 2% 1,2-dichlorobenzene, 2% nitrobenzen, Solvent: dodecane Part No. 8500-0010 0460-0186 8500-4328

Helpful Hint: Don't forget about your vials and caps for Headspace. See the General Chromatography section of this catalog.

Agilent 7694 Sampler Supplies


7694 Headspace Sampler

Description Transfer line, Silcosteel, 1 m Needle, transfer line, Silcosteel Needle, transfer line, Silcosteel Transfer line nut Transfer line ferrule

Part No. 301-211-HSP 301-222-HSP 301-221-HSP 19258-20830 19258-20870 301-017-HSP 300-301-HSP 321-055-HSP 321-056-HSP 301-220-HSP 301-015-HSP 301-016-HSP 321-002-HSP 330-086-HSP 330-055-HSP 425-553-HSP 301-169-HSP 301-212-HSP 321-058-HSP 321-059-HSP 301-011-HSP 301-152-HSP 301-013-HSP 325-045-HSP 425-554-HSP

GC and GC/MS

Oven adaptor for 10 mL vials Tray adaptors for 10 mL vials, (25/pk) Sample loop, 1 cc, nickel Sample loop, 3 cc, nickel Needle assembly vial probe, Silcosteel Needle for transfer line, 0.4 mm id, 0.8 mm od, nickel Needle for transfer line, 0.25 mm id, 0.5 mm od, nickel Restrictor, stainless steel Pressure regulator Flow controller Solenoid valve, pressure Tube, needle, 6-port valve, nickel Tube, needle, 6-port valve, Silcosteel Sample loop, 1 cc, Silcosteel Sample loop, 3 cc, Silcosteel Transfer line, 80 cm, nickel Transfer line, 1 m, nickel Needle assembly (vial probe) Zero dead volume union Solenoid valve-vent

174

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GC Instrument Parts and Supplies


Headspace Supplies/6890/6850 Split/Splitless Inlet Supplies
Agilent 7694 Sampler Supplies (continued)
Description Bulkhead fitting kit Strain relief Tube, vent-valve stainless steel Vial, 10 mL Headspace OQ/PV standard Sensor tube, 125 mm PTFE Union FF 6MB 5 piece set Union T6 MB 5 piece set, brass Union T5 MA Nut 5 mm 100/pk Unit Part No. 325-024-HSP 301-205-HSP 301-170-HSP 5182-0838 5182-9733 321-057-HSP 325-062-HSP 325-132-HSP 325-185-HSP 325-455-HSP

6890/6850 Split/Splitless Inlet Supplies


Split/Splitless Inlet Supplies*
Item Description 1 2 3 Septum retainer nut for headspace Septum retainer nut Septa, bleed temperature optimized** Insert Weldment Standard manual pneumatics Original standard EPC using 1/4 in. split vent filter Similar to G1544-60575 except allowed insertion for 1/4 in. chemical filters to clean carrier gas for ECD operation S/SL insert weldment. Used with large charcoal canister type filter, for 6890/6850 Similar to G1544-60575 except carrier lines separated for interface to valved systems of a G1540A instrument S/SL insert assembly for 1540A with valved system option. This insert assembly uses the large charcoal canister split vent filter, for 6890/6850 4 Graphite O-rings for split liner Graphite O-rings for splitless liner Fluorocarbon elastomer O-ring 12/pk 10/pk 10/pk 50/pk Unit Part No. 18740-60830 18740-60835 5183-4757 19251-60575 G1544-60575 G1544-80580
6 4 5 1 2 3

GC and GC/MS

G1544-60585 G1580-60575

G1580-60585

7 8 9

5180-4168 5180-4173 5180-4182

10

11 12 13 14

6890/6850 Series GC Split/Splitless Inlet

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175

GC Instrument Parts and Supplies


6890/6850 Split/Splitless Inlet Supplies
Split/Splitless Inlet Supplies* (continued)
Item Description
1 2 3 4 6 5

Unit 25/pk 25/pk

Part No. 5183-4647*** 5183-4702 5181-3316*** 5183-4696 G1544-60610 G1544-80530 G1544-80550 G1544-20590 18740-20880 18740-20885 5182-9652

Liner, split, low pressure drop, glass wool, single taper, deactivated Liner, splitless, Single taper, deactivated liner, 900 L

Split trap vent kit w/repl. cartridge, optional Replacement cartridge for P/N G1544-60610 Split vent trap assembly

7 8

Retaining nut SS seal Gold-plated seal Gold-plated seal with cross

9 10
7 8 9

Washer, 0.375 in. od Reducing nut Insulation (requires 3) Lower insulation cover Ferrule **** Column nut for 6890 Column nut for 6850 Angled wrench for split/splitless inlet Split/splitless injection port kit contains:

12/pk

5061-5869 18740-20800 19243-00067 19243-00070

11 12 13 14

GC and GC/MS

10

2/pk 2/pk

5181-8830 5183-4732 19251-00100 5181-8838 18740-20885 19251-60540 5062-3587 5180-4182 5181-8818 5183-4757 5180-4153

11 12 13 14

6890/6850 Series GC Split/Splitless Inlet

Gold plated seal, 3 ea. Liner, split, straight, glasswool, non-deactivated, 4 ea. Liner, splitless, single-taper, glasswool, deactivated, 2 ea. 1/4 in. Viton O-ring, 12/pk Liner, direct, 2 mm id, deactivated Bleed/Temp optimized inlet septa 11 mm, 50/pk Capillary inlet cleaning wires, 5/pk

*For a complete parts breakdown, see the 6890/6850 Series GC Instrument User and/or Service Manuals. **For a complete selection of septa, see pages 149153. ***For complete list of liners, see pages 157158. ****For a complete selection of capillary column ferrules, see pages 162163.

Gold Plated Seal P/N 18740-20885

Helpful Hint: The new optional replacement split vent trap, G1544-60610, can replace the standard 1/4 inch trap G1544-80550 using the same type of connections as the standard trap and is installed in the same location of the pneumatics carrier.
Capillary Split Vent Trap Assembly P/N G1544-80550

Optional Capillary Split Vent Trap Assembly P/N G1544-60610

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GC Instrument Parts and Supplies


6890 PTV Inlet Supplies

Programmable Temperature Vaporizing (PTV) Inlet


PTV Inlet
Item Description 1 2 3 4 5 6 7 8 Septumless head Septum head Septum nut PTV inlet assy PTV LCO2 cooling jacket PTV LN2 cooling jacket Silver seal Graphpak inlet adapter 0.20 mm 0.25-0.33 mm 0.53 mm 9 Ferrules for Graphpak inlet 0.20 mm 0.25 mm 0.32 mm 0.53 mm 10 11 Split nut for inlet adapters PTV insulation block PTV cryo insulator (not shown) Teflon ferrule (needle seal) Kalrez seal Valve body Pressure spring Viton seal Sealing element CO2 Cryo inline filter Service kit for septumless head contains Kalrez seal, valve body, and pressure spring Graphpak 3D ferrules Installation tool for 3D ferrules 5/pk 10/pk 10/pk 10/pk 10/pk 5/pk Column ID Unit Part No. G2617-60507 G2618-80500 18740-60835 G2617-60506 G2617-60508 G2619-60501 5182-9763 5182-9754 5182-9761 5182-9762 5182-9756 5182-9768 5182-9769
4 1 3 2

GC and GC/MS

5182-9770 5062-3525 G2617-20510 G2617-60510 5182-9748 5182-9759 5182-9757 5182-9758 5182-9775 5182-9760 3150-0602 5182-9747
PTV Inlet
5, 6

7 8
9 10

11

5182-9749 G2617-80540

PTV Liners
Description PTV Liner Single Baffle, 2 mm id 180 L volume, deactivated, glasswool PTV Liner Single Baffle, 2 mm id 200 L volume, deactivated PTV Liner Multi Baffle, 1.5 mm id 150 L volume, deactivated PTV Liner Fritted Glass, 1.5 mm id 150 L volume, deactivated www.agilent.com/chem Part No. 5183-2038 5183-2036 5183-2037 5183-2041

177

GC Instrument Parts and Supplies


6890 Cool On-Column Inlet Supplies

6890 Cool On-Column Inlet Supplies


Like the split/splitless inlet, the cool on-column inlet may be used with manual or automated injection. Required inlet hardware for manual injection can include either a septum nut and septum or cooling tower and duckbill septum (indicated on the part shown), along with the spring and insert. Automated injection requires the septum nut and septum, spring, and insert.

6890 Series GC Cool On-Column Inlet Supplies*


Item Description
Automatic injection

Unit

Part No.

1a 1b 2
1a 1b 1c 1d

Septum nut base for 320 mm assembly Septum nut base for 530 mm assembly Advanced green 5 mm through hole septa** BTO 5 mm through hole septa** 50/pk 50/pk

19245-80521 G1545-80520 5183-4760 5183-4758

Manual injection

1c 1d
2 3 4

Cooling tower assembly Duckbill septum 10/pk 6/pk

19320-80625 19245-40050 19091-63000 9301-0658

Fused silica syringe needles Syringe barrel for use with fused-silica needles,10 L

GC and GC/MS

Common Supplies

3 4

Spring Inserts For 200 m columns (one ring) For 250 m columns (six rings) For 320 m columns (five rings) For 530 m columns (no rings) For 530 m Al clad columns (four rings)

19245-60760 19245-20510 19245-20515 19245-20525 19245-20580 19245-20780 5080-8853 2/pk 5181-8830

5
5 6

Ferrule*** Column nut

6890 Cool On-Column Inlet

*For a complete parts breakdown, see the 6890 Series GC Instrument User and/or Service Manuals. **For a complete selection of septa, see pages 149153. ***For a complete selection of ferrules, see pages 162163.

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GC Instrument Parts and Supplies


6890 Cool On-Column Inlet Supplies

6890 Cool On-Column Inlet Supplies


Be sure to select a needle, septum nut, and insert based on the inside diameter of the column selected. Use the following tables to make this determination for automatic and manual injections.

Automatic or Manual Injection Using Stainless Steel Needles


Column Type and ID Fused silica Needle Part No.* Price Septum Nut** Part No.* Price Insert Part No.

530 m

5182-0832

$27

G1545-80520

$58

19245-20580

320 m

5182-0831

43

19245-80521

57

19245-20525 (5 rings)

Programmable COC, Septum Nut, 250/320 m P/N 19245-80521

250 m

5182-0833

43

19245-80521

57

19245-20515 (6 rings)

200 m

Use cooling and tower duckbill septum

19245-20510 (1 ring)
Programmable COC Septum Nut, 530 m P/N G1545-80520

GC and GC/MS

Aluminum clad

530 m

5182-0832

27

G1545-80520

58

19245-20515 (4 rings)

*All needles use 5 L syringe barrel, P/N 5182-0836. **Use a solid septum, P/N 5181-1261, for manual injection. Use through-hole septum, P/N 5181-1260, for automatic injection.

Column/Retention Gap Installation Consumables


Item Description Column nut 250 m graphite/Vespel ferrule 320 m 0.5 mm graphite/Vespel ferrule 250 m retention gap (one 5 m piece) 320 m retention gap (one 5 m piece) 530 m retention gap (one 5 m piece) Quartz deactivated column connector fits 0.18-0.53 mm 5/pk Unit 2/pk 10/pk 10/pk Part No. 5181-8830 5181-3323 5062-3514 160-2255-5 160-2325-5 160-2535-5 5181-3396

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179

GC Instrument Parts and Supplies


6890/6850 Packed Port Inlet Supplies

6890/6850 Packed Port Inlet Supplies


Selecting Adapters and Liners for Packed Inlets
1 2 3 4

For purged-packed inlets, adapter selection is dependent on the type of column used: 1/4 in. packed, 1/8 in. packed, or 0.53 mm. It allows the columns to be connected to the inlet. Glass liners are often used with metal adapters to reduce reactivity and trap nonvolatile residues. Liners are installed from the top of the inlet and should be installed before the column.

6890 Series GC Packed Port Supplies*


Item Description 1 2 3 4 5 6 7 Septum nut Septa Bleed temperature, optimized,** 11 mm Top insert weldment Viton O-rings Glass liner, disposable Glass liner, disposable/deactivated
Liner

Unit 50/pk 12/pk 25/pk 5/pk 10/pk 10/pk

Part No. 18740-60835 5183-4757 19243-80570 5080-8898 5080-8732 5181-3382 5080-8774 5180-4105

Vespel/graphite ferrules, 1/4 in. id Tubing nut, 1/4 in. brass

Adapters with glass liners

GC and GC/MS

8
5

0.53 mm column adapter 1/8 in. column adapter 1/4 in. column adapter

19244-80540 19243-80530 19243-80540 19243-00067 19234-60720 2/pk 5181-8830

6 7 8 1/4 in. Vespel/ graphite ferrule 1/4 in. nut 1/8 in. or 1/4 in. stainless steel liner

9 10 11

Upper insulation Nut warmer cup with insulation Column nut for 0.53 mm column

Inside of Oven 9 10

*For a complete parts breakdown, see the 6890 Series GC Instrument User and/or Service Manuals. **For a complete selection of septa see pages 149153.

Installation Matrix for Packed Metal Columns


11

6890 Series GC Packed Port Inlet

Inlet or Detector

1/4 in. Columns Where to Install

Key Points

1/8 in. Columns Where to Install

Key Points

Purged packed inlet Adaptable NPD* or FID ECD TCD

1/4 in. liner

1/8 in. liner

1/4 in. adapter Remove or install 1/8 in. adapter (P/N 19231-80530) adapter as desired (P/N 19231-80520) Detector fitting 1/4 in. adapter (P/N G1532-20710) Remove adapter if necessary 1/8 in. adapter (P/N 19301-80530) Detector fitting Remove adapter if necessary

*Do not remove plugs from your NPD until you are ready to connect the column and operate the detector.

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GC Instrument Parts and Supplies


6890/6850 Detector Supplies

6890/6850 Series GC Detector Supplies


Detectors available for use with 6890 and 6850:
Flame ionization detector (FID) for packed or capillary columns Thermal conductivity detector (TCD) Mass-selective detector (MSD)

6890 only:
Electron capture detector (ECD) Nitrogen-phosphorus detector (NPD) for packed or capillary columns

GC Detector Specifications
GC/ Detector Max Op Temp Sensitivity (MDL) Dynamic Range Makeup Gas Types Selectivity

6890 GC/FID and 6850 GC/FID 6890 GC/NPD

450C 375C 400C

<5 pg carbon/sec as propane using N2, 0.2794 mm jet <0.4 pg N/sec, <0.2 pg P/sec with azobenzene/ malathion mixture <400 pg propane/mL He <0.04 pg/sec lindane

Linear: <10%, with N2 and 0.2794 mm jet >105 N, >105 P with azobenzene/ malathion mixture Linear: 105 (5%) 104 with lindane

N2 H He

not applicable

35000 to 1 gN/gC 75000 to 1 gN/gC not applicable

6890 GC/TCD and 6850 GC/TCD 6890 GC/ECD

400C 375C 400C

He N2 He N2 Ar He N2 Ar

GC and GC/MS

6890 GC/MSD 6850 GC/MSD

320C (Interface)

Full scan: 10:1 RMS on 10 pg hexachlorobenzene (splitless mode) Selected monitoring: 10:1 RMS on 200 pg hexachlorobenzene (splitless mode)

Linear: 104 Total ADC: 106

not applicable

Note: All 6890 Series GC detectors are available with manual or electronic regulation of gases; and all detectors, both manual and electronic, include electronic ON/OFF gas switching from the 6890 keyboard.

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181

GC Instrument Parts and Supplies


6890/6850 FID Supplies

6890/6850 Flame Ionization Detector (FID) Supplies


Item Description
1

Unit

Part No. 19231-21050 19231-20940

1 2 3 4
5

PTFE chimney (optional)** Collector nut Spring washer Ignitor castle Optional Hastelloy Ignitor Castle* Ignitor glow plug assembly Collector insulator Collector body FID collector assembly 1 Detector insulation assembly FID collector cleaning brush Collector Housing FID retainer nut wrench (5880, 5890, 6890) 2/pk 10/pk

2 3

5181-3311 19231-20910 19231-21060 19231-60680 G1531-20700 G1531-20690 G1531-60690 G1531-20700 8710-1346 G1531-20740 19301-00150 8710-1561 5182-3450 18710-20119 18710-60170 19231-60680 19244-80560 19301-60660 5180-4150 5180-4152 19301-20720 5180-4153 9301-0985

4 8 6 7

5 6 7 8

GC and GC/MS

1/4 in. Nut Driver for FID jet-drilled shaft FID supplies kit-Includes: Jet, packed standard, 3 each FID performance evaluation sample kit, 2 each Ignitor glow plug assembly, 2 each Jet, cap. series 530 m, 3 each FID flow measuring insert, 2 each Cleaning wires for 0.03 in. id jet Cleaning wires: 0.018 in. id/530 m jet, 1/pk Wire, jet cleaning, 5 each Capillary inlet cleaning wires Detector cleaning kit

6890 Series GC FID Detector

Collector Body P/N G1531-20690

*Hastelloy components may be used when analyzing highly corrosive materials. **Not included in assembly. 1 Does not include Hastelloy component.

FID Jets
Description
Jets for capillary dedicated FID

Length (mm) 48 48

Part No. G1531-80560 G1531-80620

Capillary jet (0.011 in./0.29 mm id tip) High-temperature jet (SimDIS 0.018 in. id tip)

182

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GC Instrument Parts and Supplies


6890/6850 FID Supplies
FID Jets (continued)
Description
Adaptable FID

Length (mm) 61.5 63.5 63.5 61.5

Part No. 19244-80560 18710-20119 18789-80070 19244-80620


FID Jet

Capillary 0.53 mm jet (0.011 in. id tip) Packed (0.018 in. id tip) Packed (0.030 in. id tip) 0.53 mm high temp (0.018 in. id tip) for SimDIS

FID Consumables
Description FID flow measuring insert FID ignitor cable FID Performance evaluation sample kit, 0.03% C14, C15, and C16 normal alkanes in hexane O-rings Cleaning wire Jet cleaning wire for 0.03 in. id jet 5/pk Jet cleaning wire for Series 530 mm and 0.018 in. id jet 5/pk 12/pk Unit Part No. 19301-60660 G1531-60680 18710-60170 5080-4978 19301-20720 5180-4150 5180-4152

GC and GC/MS

Cleaning Your FID Jet


It is often more convenient to replace dirty jets than to clean them, especially if they have been badly contaminated. However, if you decide to clean a jet, be careful not to scratch the jet internally; scratches will ruin the jet. To be on the safe side, you may want to clean the jet using an aqueous bath only. To do this you will need the following materials: Small ultrasonic cleaning bath Aqueous detergent GC-grade methanol in a Teflon wash bottle Flame detector cleaning kit (P/N 9301-0985) Dry, filtered compressed air or nitrogen Forceps or tweezers 1. Run a cleaning wire through the top of the jet. Run it back and forth a few times until it moves smoothly. Be careful not to scratch the jet. 2. Fill the ultrasonic cleaning bath with aqueous detergent, and place the jet in the bath. Sonicate for five minutes. 3. Use a jet reamer to clean the inside of the jet. 4. Sonicate again for five minutes. Note: From this point on, handle the parts only with forceps! 5. Remove the jet from the bath and rinse it thoroughly first with hot tap water and then with a small amount of the methanol. 6. Blow the jet dry with a burst of the compressed air or nitrogen, and then place the jet on a paper towel and allow it to air dry.

FID Cleaning Kit P/N 9301-0985

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183

GC Instrument Parts and Supplies


6890 NPD Supplies

6890 Nitrogen Phosphorus Detector (NPD) Supplies


The NPD for the 6890 GC features a ceramic bead selective for nitrogen and phosphorus compounds. All Agilent NPD beads are preconditioned and come with a proof-of-performance chromatogram. The redesigned NPD bead is self-aligning for easy installation.
NPD Ceramic Bead

Like the FID, there are two types of NPD is available. The capillary optimized NPD is only used with capillary columns and the adaptable NPD fits packed columns and can be adapted to fit capillary columns.
2

6890 NPD Supplies


Item Description 1 2 Screws, M3 x 0.5 x 8 mm (Pozidriv) NPD white ceramic bead assembly* NPD black ceramic bead assembly** 3 4 5 6 7 8 9 10 11 12 13 14 Lid weldment Metal C-ring, top Alumina insulator, upper Collector funnel Alumina insulator, lower Metal C-ring bottom Screw, M4 x 10 mm J-Clamp Screw, M4 x 10 mm NPD interconnect assembly Mounting pallet
Jets

Part No. 0515-0655 G1534-60570 5183-2007 G1534-80510 0905-2580 G1534-40020 G1534-20530 G1534-40030 0905-1284 0515-2495 1400-0015 0515-2495 G1534-60610 G1531-40020 G1531-80560 19244-80560 18710-20119
G1534-80500

9 10 4 5 6 12

11

GC and GC/MS

13 14

15 16

Fused silica capillary (0.011 in.), dedicated, 48 mm length Packed capillary (0.011 in.), 61.5 mm length Packed column (0.018 in.), 63.5 mm length 15
Base weldment, Capillary NPD

17

18

Base Weldment, Packed NPD 16 Lid stop NPD Ceramic Insulator Kit includes items 4, 5, 7, and 8

G1534-80540 G1534-20590 5182-9722

*This bead is more sensitive but exhibits some tailing for phosphorous compounds. Quantity discounts available. **The black bead is potentially a little less sensitive but does not exhibit peak tailing and typically has longer lifetime.

6890 Series GC NPD Detector

184

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GC Instrument Parts and Supplies


6890 NPD/6890/6850 TCD Supplies
Item Description 17
Column adapters for packed NPD

Unit

Part No. 19244-80610 19231-80520 19231-80530 19234-60720

Capillary column 1/8 in. packed column 1/4 in. packed column 18 Nut warmer cup with insulation 1/4 in. Vespel ferrule 1.0 mm graphite ferrule 0.5 mm graphite ferrule 1/4 in. brass tubing nut Column nut NPD chemical sample kit solution of 0.65 ppm azobenzene, 1000 ppm octadecane, 1 ppm malathion in isooctane, 3 ampoules 10/pk 10/pk 10/pk 10/pk 2/pk

5080-8774 5080-8773 5080-8853 5180-4105 5181-8830 18789-60060

6890/6850 Thermal Conductivity Detector (TCD) Supplies


Item Description 1 2 3 4 5 TCD replacement cell TCD cap column adapter seal TCD cap column adapter Graphite ferrules, 1.0 mm id Graphite ferrules, 0.5 mm id Capillary column nut, for 6890 Capillary column nut, for 6850
Miscellaneous hardware for packed columns

Unit 10/pk 10/pk 10/pk 2/pk 2/pk 10/pk 10/pk 10/pk 10/pk

Part No. G1532-60675 18740-20950 18740-20960 5080-8773 5080-8853 5181-8830 5183-4732 5080-8774 0100-1332 5180-4103 5180-4105 G1532-20710 18711-60060
Agilent GC TCD
2 3 4 1

GC and GC/MS

Vespel/graphite ferrules, 1/4 in. id Vespel ferrules, 1/8 in. id Tubing nut, 1/8 in. brass Tubing nut, 1/4 in. TCD packed column adapter TCD chemical sample kit, 3 ampoules, 0.5 mL, solution of 0.3% C14, C15, and C16, normal alkanes in hexane

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185

GC Instrument Parts and Supplies


6890/6850 TCD Supplies TCD Column Ferrule Sealing System
Beginning in early 1999 the TC detector for the 6890 Series GC began using a new method of sealing the column into the base of the detector. The new fitting, which reduces noise slightly, forms a better seal without air leaks. It may still require retightening after wide temperature programming ranges. This new ferrule system improves the analytical results for samples that may react with oxygen. It consists of a brass back ferrule, a graphite/Vespel front ferrule, which is available in three different hole sizes and a no hole version. The same size brass back ferrule is used for all column sizes and is reusable over a long lifetime. Step 1: The column is placed through the nut first and then the back ferrule. Step 2: The front ferrule is placed next and must have the tapered end towards the detector (just like a standard ferrule) Step 3: The protrusion in the front ferrule must enter into a matching recess in the back ferrule. Tighten the fitting until it holds the column enough to resist a pull on the column to remove it. Step 4: Tighten the fitting until it holds the column enough to resist a pull on the column to remove it. Below is a list of part numbers, which will allow you to use the new system with all of your columns.
Column ID 0.53 mm 0.32 mm 0.25 / 0.2 / 0.1 mm No hole Back Ferrule (10/pk) 5182-3477 5182-3477 5182-3477 5182-3477 Price $44 44 44 44 Front Ferrule (10/pk) 5182-9673 5182-9676 5182-9677 5182-9679

GC and GC/MS

Note: It is still possible to use the older system of sealing, which will require the purchase of the adapter fitting (P/N G1532-80540), now requiring two sealing ferrules.

Column

TC Detector

Front ferrule

Back ferrule

1/8" Brass nut

186

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GC Instrument Parts and Supplies


6890 ECD Supplies/FPD Supplies
6890 Electron Capture Detector (ECD) Supplies
Item 1 2, 3 Description ECD makeup gas adapter* Nut warmer cup with insulation ECD adapter replacement liner, fused silica ECD end cap ECD chemical sample kit, 3 ampoules solution of 33 pg/mL (0.033 ppm) each of lindane and aldrin in isooctane ECD wipe test kit Part No. G1533-80565 19234-60720 19233-20625 19233-20755 18713-60040

18713-60050
*Includes one each of P/N 19233-20625 and P/N 19233-20755

ECD Wipe Test: A wipe test kit (P/N 18713-60050) is supplied with each new ECD. The kit includes an information card with instructions for performing the test. Records of tests and results must be maintained for possible inspection by the NRC (Nuclear Regulatory Commission) and/or responsible state agency.

6890 Series GC ECD Detector

6890 Flame Photometric Detector (FPD) Supplies


Item Description 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 Exit tube assembly Nut, brass, 1/4 in. Ferrule, Vespel/graphite 1/4 in. id Weldment, block O-ring, ignitor, Kalrez Spacer, ignitor Glow plug Ignitor cable assembly Gasket, heat shield Window, first heat shield Disk, heat shield Coupling, SS Lockwasher (4 required) Screw, M3 x 12 (4 required) Clamp O-ring, inner window Window, second heat shield O-ring, outer window Flange adapter Flange ring O-ring, Viton, 1.239 in. id 12/pk 12/pk 12/pk Unit Part No. 19256-60700 5180-4105 5080-8774 19256-80560 0905-1102 19256-20590 0854-0141 G1535-60600 19256-80040 19256-80030 19256-20580 19256-20550 2190-0108 0515-0911 19256-00090 5061-5886 19256-80060 5061-5891 19256-20510 19256-00200 5061-5890
16 17 18 19 20 21 8 7 6 5 4 9 10 11 1 2 3

GC and GC/MS

12 13 14

15

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187

GC Instrument Parts and Supplies


5890 Split/Splitless Inlet Supplies

5890 Inlet Supplies


1 2 3 14 13 12 11 2 4

Split/Splitless Multimode Inlet Supplies


Item Description 1 2 3 Septum retainer nut or Retainer nut for headspace sampling Split/splitless insert weldment with tubing Graphite O-ring for split liner Graphite O-ring for splitless liner Fluorocarbon elastomer O-ring for liners 4 5 6 Inlet liners, split, glass wool** Retaining nut SS seal Gold plated seal Gold plated seal, cross 7 8 9 10 Washer Reducing nut Insulation (must order quantity 3) Lower insulation cover 12/pk 12/pk 10/pk 10/pk Unit Part No. 18740-60835 18740-60830 19251-60575 5180-4168 5180-4173 5180-4182 19251-60540 19251-20620 18740-20880 18740-20885 5182-9652 5061-5869 18740-20800 19243-00067 19243-00070

5 6 7 8

10

5890 Split/Splitless Inlet

GC and GC/MS

For Split/Splitless Inlets Using Electronic Pressure Control

11 12 13 14

Splitter tube* Front ferrule, 1/8 in. brass Back ferrule, 1/8 in. brass Tubing nut, 1/8 in. brass 10/pk 10/pk 10/pk 5/pk

19251-80525 5180-4109 5180-4115 5180-4103 19251-00100 5180-4153 8500-4789

Angled wrench for split/splitless inlet Capillary inlet cleaning wires


Capillary Inlet Evaluation Sample (Split Mode):
Gold Plated Seal P/N 18740-20885

Solution of 1.0% of nonane (C9), hexadecane (C16), in tetradecane (C14). Three ampoules.
*Includes 1 each of P/N 5180-4109, 5180-4115, 5180-4103. **For a complete selection of inlet liners, see pages 157158.

Helpful Hint: For optimum gas chromatographic performance, see our complete selection Inlet Septa pages 149153.

188

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GC Instrument Parts and Supplies


5890 Split/Splitless Inlet Supplies
Split/Splitless Multimode Inlet Kits
Item Description Split/Splitless Injection Port Kit includes: Gold plated seal Linear, split, with cup, glass wool and packing Liner, split, straight, glass wool, non-deact Ferrule install tool Blanking plug, finger-tight style Washers 0.375 od Liner, splitless, single-taper, glass wool, deact Ferrule, graphite 530 m, 1.0 mm id Ferrule, graphite 320 m, 0.5 mm id Graphite O-ring, 5700/5840/6890 6.52 mm id, 9.63 mm od, 0.62 in. od seals O-rings, ungreased for trap fitting 1/4 in. Viton O-ring Glass wool, pesticide grade Plastic fitting Liner, direct, 2 mm id, deactivated Column nut Capillary tubing cutter Bleed/temp optimized inlet septa 11 mm Tool, wrench Capillary inlet supplies kit includes: Gold plated seal Liner, split, straight, glass wool, non-deact. Liner, splitless, single-taper, glass wool, deact. 1/4 in. Viton O-ring Liner, direct, 2 mm id, deactivated Bleed/Temp optimized inlet septa 11 mm 50/pk 3 each 4 each 2 each 12/pk 10/pk 2 each 2/pk 4/pk 50/pk 2 each 12/pk 3 each 10/pk 10/pk 10/pk 10/pk 12/pk 12/pk 3 each 3 each 3 each Unit Part No. 5181-8838 18740-20885 18740-60840 19251-60540 19251-80680 5020-8294 5061-5869 5062-3587 5080-8773 5080-8853 5180-4168 5180-4173 5180-4181 5180-4182 5181-3317 5181-3394

GC and GC/MS

5181-8818 5181-8830 5181-8836 5183-4757 8710-0510 5182-3449 18740-20885 19251-60540 5062-3587 5180-4182 5181-8818 5183-4757

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189

GC Instrument Parts and Supplies


5890 Cool On-Column Inlet Supplies
5890 Cool On-Column (COC) Inlet Supplies
Item Description
Manual Injection

Unit

Part No. 19320-80625

1 2 3 4

Cooling tower assembly Duckbill Spring Inserts for capillary columns: For 530 m columns (no rings) For 530 m Al clad columns (4 rings) For 320 m columns (5 rings) For 200 m columns (1 ring) 10/pk

19245-40050 19245-60760 19245-20580 19245-20780 19245-20525 19245-20510

2 3 4
Cool On-Column Inlets for Manual Injection

Fused silica syringe needles Syringe for use with fused silica needles Automatic Injection 1a 1b 2 Septum nut (5890 Series II GC) Needle guide (for 7673A only) Septa Advanced Green 5 mm, through-hole* Septa, BTO 5 mm, solid manual, high pressure* Spring Inserts for capillary columns: For 530 m columns (no rings) For 320 m columns (5 rings) For 250 m columns (6 rings)

6/pk

19091-63000 9301-0658 19245-80520 19245-20670

50/pk 50/pk

5183-4760 5183-4758 19245-60760 19245-20580 19245-20525 19245-20515

GC and GC/MS

3 4

1B 1A 2 3

Common Supplies

5 6

Weldment (refer to instrument manuals) Capillary Column Ferrule** Column nut 2/pk 5080-8853 5181-8833

Column/Retention Gap Installation Consumables

320 m Vespel/graphite ferrule 250 m Vespel/graphite ferrule 320 m retention gap (one 5 m piece) Deactivated quartz column connector, fits 0.18-0.53 mm

10/pk 10/pk 5/pk

5062-3514 5181-3323 160-2325-5 5181-3396

6 7
5890 COC Inlet

*For a complete selection of septa see pages 149153. **For a complete selection of capillary column ferrules see pages 162163.

190

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GC Instrument Parts and Supplies


5890 Packed Column Inlet Supplies
5890 Packed Column Inlet Supplies
Item Description Purged Packed Column Inlet Kit: Includes Ferrule Vespel/graphite 1/4 in. for inserts/glass columns 10/pk O-ring, Viton Disposable glass insert 1 Septum retainer nut Headspace septum retainer nut Nonpurging septum nut assembly for manual flow control only, not EPC 2 3 4 5 6 7 8 Septa, BTO 11 mm od Top insert weldment Fluorocarbon elastomer O-rings Weldment (refer to instrument manuals) Vespel ferrules Tubing nut, 1/4 in. brass Adapters without glass liners: 1/8 in. column adapter 1/4 in. column adapter Adapters with glass liners: Agilent Series 530 mm column adapter 1/8 in. column adapter 1/4 in. column adapter Glass liner, disposable, 170 L internal volume Deactivated glass liner, disposable, 170 L internal volume
Universal Packed Column Inlet (non-purged)

Unit

Part No. 5181-8837 5080-8774 5080-8898 5181-3382 18740-60835 18740-60830 19243-60570

12/pk 5/pk

50/pk 12/pk 10/pk 10/pk

5183-4757 19243-80570 5080-8898 5080-8774 5180-4105 19243-80510 19243-80520 19244-80540 19243-80530 19243-80540
Septum-Purged Packed Column Inlet

GC and GC/MS

25/pk 5/pk

5080-8732 5181-3382 19243-60505

1 2 3 4 5 6

Septum retainer nut for headspace sampling, nonpurging Septa, BTO 11 mm od Weldment (refer to instrument manuals) Graphite/Vespel ferrule Brass nut, 1/4 in. Adapters without glass liners: 1/8 in. column adapter 1/4 in. column adapter Adapters with glass liners: Agilent Series 530 mm column adapter 1/8 in. column adapter 1/4 in. column adapter 10/pk 10/pk 50/pk

5183-4757 5080-8774 5180-4105 19243-80510 19243-80520 19244-80540 19243-80530 19243-80540


Universal Packed Column Inlet

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191

GC Instrument Parts and Supplies


5890 FID/NPD Supplies
Universal Packed Column Inlet (non-purged) Supplies (continued)
Description Headspace septum nut, non purged inlet, non EPC Deactivated glass liners, disposable, 170 L internal volume 5/pk Glass liner, disposable, 170 L internal volume 25/pk Unit Part No. 19243-60500 5181-3382 5080-8732

5890 Detector Supplies


Flame Ionization and Nitrogen Phosphorus Detector Supplies
2 3 4 5 6

Item Description
FID Supplies

Unit

Part No.

1 2 3 4 5 6 7

Collector assembly (contains items 2-9) PTFE chimney (optional) Collector nut Spring washer Ignitor castle Optional Hastelloy ignitor castle Ignitor (glow plug) assembly Upper collector insulator Collector body Optional hastelloy collector Lower collector insulator Detector nut warmer (not shown) FID performance evaluation sample kit: Solution of 0.03% C14, C15, and C16 in hexane, 3 ampoules, 0.5 mL volume FID supplies kit-Includes: Jet, packed standard 0.018 in. id tip FID performance evaluation sample kit Ignitor glow plug assembly Jet, capillary series 530 m, 0.011 in. id tip FID flow measuring insert Cleaning wires for 0.03 in. id jet Cleaning wires: 0.018 in. id/530 m jet Wire, jet cleaning Capillary inlet cleaning wires Detector clean kit 3 each 2 each 2 each 3 each 2 each 5/pk 5/pk 5 each 5/pk 10/pk

19231-60690 19231-21050 19231-20940 5181-3311 19231-20910 19231-21060 19231-60680 19231-20970 19231-20960 19231-21080 19231-20950 19234-60720 18710-60170

8 9

GC and GC/MS

5890 FID Supplies

8 9

5182-3450 18710-20119 18710-60170 19231-60680 19244-80560 19301-60660 5180-4150 5180-4152 19301-20720 5180-4153 9301-0985

Helpful Hint: Hastelloy components should be used when analyzing highly corrosive materials such
as chlorinated solvents for HCl via flame.

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GC Instrument Parts and Supplies


5890 FID/NPD Supplies
Flame Ionization and Nitrogen Phosphorus Detector Supplies (continued)
Item Description FID flow measuring insert FID collector cleaning brush 1/4 in. nut driver for 5890A FID jet, drilled shaft
Jets:

Unit 2/pk

Part No. 19301-60660 8710-1346 8710-1561

Capillary/Series 530 mm jet (0.011 in. id tip) Packed standard 0.018 in. id jet Packed wide-bore 0.030 in. id jet (for high-bleed applications) High-temperature jet (for simulated distillation) FID flow measuring adapter O-ring for flow adapter Jet cleaning wire for 0.03 in. id jet Jet cleaning wire for Series 530 mm and 0.018 in. id jet
NPD Supplies

19244-80560 18710-20119 18789-80070 19244-80620 19301-60660 5080-4978 5/pk 5/pk 5180-4150 5180-4152

NPD collector (NPD bead) Recoating kit, sufficient for 10 collectors NPD sample: solution of 0.65 ppm azobenzene, 1,000 ppm octadecane, and 1.00 ppm malathion in isooctane, 3 ampoules, 0.5 mL volume
FID and NPD Adapters

19234-60540 5080-8872 18789-60060

Adapters
5 6 2

GC and GC/MS

1 2 3 4 5

FID/NPD 1/4 in. packed column FID/NPD 1/8 in. packed column FID/NPD capillary column FID/NPD capillary column nut FID/NPD Vespel ferrule, 1/4 in. FID/NPD graphite ferrule, 1.0 mm (not shown) Graphite ferrule, 0.5 mm (not shown) 2/pk 10/pk 10/pk 10/pk 10/pk

19231-80530 19231-80520 19244-80610 5181-8830 5080-8774 5080-8773 5080-8853 5180-4105

1/8 in. Packed Column Use 5 6 1

1/4 in. Packed Column Use 5 6 3 4

Tubing nut, 1/4 in. brass

Capillary Column Use

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193

GC Instrument Parts and Supplies


5890 TCD/ECD Supplies
7

3 4

Thermal Conductivity Detector TCD Supplies


Item Description Unit Part No.
TCD

1
2 8 1

Capillary column nut Graphite ferrule, 0.5 mm id Graphite ferrule, 1.0 mm id Vespel ferrule, 1/8 in. id Tubing nut, 1/8 in. brass Makeup gas adapter, TCD TCD column adapter for capillary columns TCD column adapter seal TCD column adapter, 1/8 to 1/4 in. glass

2/pk 10/pk 10/pk 10/pk 10/pk

5181-8830 5080-8853 5080-8773 0100-1332 5180-4103 19232-80550 18740-20960 18740-20950 19302-80020 19232-60676 18711-60060

2 3

TCD Capillary Adapter without Make up

TCD for Packed or 1/4 in. Glass Column

4 5 6 7 8

TCD replacement cartridge TCD performance evaluation sample: C14, C15, and C16 (0.3%) in hexane, 3 ampoules, 0.5 mL volume
TCD Adapter with Make up

Electron Capture Detector ECD Supplies


Item Description Unit 10/pk 10/pk Part No. 5080-8774 0100-1331 5180-4105 19301-80530 19233-80565 G2397-80520 19233-20625 G2397-20540 19233-20755 18713-60040 1 2 Vespel graphite ferrule, 1/4 in. id Vespel/graphite 85%/15% ferrule, 1/4 in. id Tubing nut, 1/4 in. brass ECD column adapter, 1/4 to 1/8 in. metal Makeup gas adapter, ECD, new style Makeup gas adapter for micro ECD adapter ECD fused silica liner (new style) ECD adapter fused silica liner micro ECD Stainless steel cap for ECD makeup gas adapter (new style and micro ECD)

GC and GC/MS

12 11

1 2

3 5 10 11 12

5, 10

ECD Make up Gas Adapter

ECD Packed for 1/4 in. Glass Columns

ECD Performance evaluation sample lindane and aldrin (0.033 ppm) in Isooctane 3 ampoules, 0.5 mL

Helpful Hint: To increase your GC performance, see our complete line of gas specific or contaminant specific purifiers.

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GC Instrument Parts and Supplies


5890 FPD Supplies
Flame Photometric Detector FPD Supplies
Description Adapter weldment, 1/8 in. columns Adapter weldment, capillary Start-up kit Exit tube Window O-ring, inner Window O-ring, outer Window, heat shield, 1st Window, heat shield, 2nd Ferrule, 1/4 in., Vespel Glow plug FPD O-ring O-ring, Viton Liner/ferrule kit Sulfur filter Phosphorus filter O-ring, greased Kalrez O-ring, size 2-002 Kalrez O-ring, size 2-010 Kalrez O-ring, size 2-011 Fluorocarbon Elastomer O-ring, brown, 0.926 in. id Fluorocarbon Elastomer O-ring, brown, 1.239 in. id FPD Performance Evaluation Sample: Solution of 20 ng/mL (20 ppm) dodecanethiol and tributylphosphate in isooctane, 3 ampoules 12/pk 12/pk 12/pk 12/pk 12/pk 10/pk 12/pk 12/pk Unit Part No. 19256-80590 19256-80570 19256-60500 19256-60700 5061-5886 5061-5891 19256-80030 19256-80060 5080-8774 0854-0141 5061-5867 5180-4182 19256-60590 19256-80000 19256-80010 5080-8846

GC and GC/MS

0905-1101 0905-1102 0905-1103 5061-5889 5061-5890 19305-60580

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195

GC Instrument Parts and Supplies


AED Supplies

G2350 Atomic Emission Detector Supplies


The G2350A Atomic Emission Detector Brings Speed and Selectivity
Environmental laboratories are using the Atomic Emission Detector (AED) to make GC analysis of unknowns faster and more accurate. The AED quickly and efficiently screens for a wide range of substances without losing unknown compounds. At picogram levels, the AED selectively detects contaminants containing Cl, N, S, and P without interference from elements such as phthlates and ketones. The Agilent AEDs are also able to detect organometallic compounds containing Hg, Fe, Sn, As, Ni, V, Se, and Si. Atomic emission detection makes routine work out of the most difficult analyses.

5921 Atomic Emission Detector

G2350 AED Supplies


Description Lens keeper O-ring, Viton Gas Union 1/16 in. tubing nut, SS Discharge Tube Unit 12/pk 10/pk Part No. 5061-5889 5180-4102 G2350-61090

GC and GC/MS

5921/G2350A AED Supplies


Description Window O-ring Spectrometer window replacement kit Gas union brazement Purge manifold assembly 0.53 mm ferrules, brass 0.32 mm ferrules, brass 0.20 mm ferrules Plug ferrules 1/16 in. to 0.5 mm Vespel Graphite reducing ferrule 1/16 in. to 0.4 mm Vespel Graphite reducing ferrule Ferrule nut Ferrule key Discharge Tube 10/pk 10/pk 10/pk 10/pk 10/pk 10/pk Unit Part No. 0905-1161 05921-80350 05921-80600 05921-61390 5021-7134 5021-7137 5021-7136 5021-7133 5062-3506 5062-3508 05921-21850 05921-21860 05921-61090

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GC Instrument Parts and Supplies


AED Supplies
Agilent 5921/G2350A Common Supplies
Description GC chamber O-ring Optical chamber O-ring Receiver liner O-ring (large) Receiver liner fitting nut Receiver liner ferrules Receiver liner O-ring (small) 1/8 in. Teflon ferrules 1/4 in. Vespel ferrules 1/16 in. Vespel ferrules Nut, 1/16 in. SS Transfer line 0.5 mm graphite ferrule Fly ash filter 1/4 in. filter, 5 m water filter element (internal water system only) 1/4 in. filter, 60 m water filter element (external water system only) Cavity brush, small Cavity brush, large AED performance sample Sample 1: Contains (w/w) 0.07% nitrobenzene, 0.43% n-tridecane, 0.07% 4-fluoroanisole, 0.05% tert-butyldisulfide, 0.08% 1,2, 4-trichlorobenzene, 0.05% tetraethyl orthosilicate, 0.05% n-decane (perdeuterated), 4.3% n-dodecane, 0.13% n-tetradecane, 0.07% 1-bromohexane, 4.04% n-octane in isooctane. Sample 2: Contains the same components as Sample 1 plus 0.06% triethylphosphate. Six 0.5 mL ampoules. Three of each sample. AED check-out column (HP-1) 25 m, 0.32 mm, 0.17 m 19091Z-012 10/pk 10/pk 10/pk 10/pk 10/pk Unit Part No. 0905-1159 0905-1160 0905-1163 0100-1505 0100-1504 0905-1162 0100-1365 0100-1327 0100-1329 5180-4102 5080-8853 3150-0417 3150-0617 3150-0374 8710-1876 8710-1938 8500-5067

GC and GC/MS

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197

GC Instrument Parts and Supplies


6890/5890 Regulators, Mass Flow Controllers, and Restrictors
Backpressure Regulators
Description 0-15 psig backpressure regulator/gauge kit 0-60 psig backpressure regulator/gauge kit 0-15 psig (0-100 kPa) 0-30 psig (0-200 kPa) 0-60 psig (0-400 kPa)
Backpressure Regulator

Part No. 19246-60620 19246-60630 19246-60560 19246-60570 19246-60580 19246-60690

0-100 psig (0-700 kPa)

Pressure Gauges
Description 0-15 psig (0-100 kPa) 0-30 psig (0-200 kPa) 0-60 psig (0-400 kPa) 0-100 psig (0-700 kPa) Part No. 19246-60526 19320-60656 19363-60506 19361-60566

Mass Flow Controllers


Description Part No. 19362-60565 19362-60575 Mass flow controller with 20 mL/min restrictor for use with packed column inlets Mass flow controller with 400 mL/min restrictor for use with capillary inlets

GC and GC/MS

Restrictor-New Style
Description 0-20 mL/min 0-60 mL/min 0-110 mL/min
Mass Flow Controller Restrictor (old style screw type)

Color Dot Code Red and Purple Blue and Purple Green and Purple Black and Purple Yellow and Purple

Part No. 19362-60515 19362-60525 19362-60535 19362-60545 19362-60555

0-400 mL/min 0-750 mL/min

Restrictor-Old Style
Description 0-20 mL/min 0-60 mL/min 0-110 mL/min 0-400 mL/min 0-750 mL/min Color Code Red Red Green Black Silver Green Color Dot Silver Part No. 19362-60510 19362-60520 19362-60530 19362-60540 19362-60550

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GC/MS Parts and Supplies


MSD Maintenance Schedule

Maintaining Mass Selective Detectors (MSD)


Your GC/MSD system contains a high level of sensitivity. To continue achieving optimal results, it is critical to maintain your system properly by performing the essential tasks within this section. Some of the benefits for maintaining your GC/MSD include: less downtime for repairs a longer lifetime for your MSD system a reduction in overall operating costs

Maintenance Schedule
Some parts of the MSD require regularly scheduled maintenance. Common maintenance tasks are listed below. It is advisable to keep a log book of system performance, Autotune, and maintenance operations performed. This makes it easier to identify variations from normal performance and to take corrective action.

Every Day
Check, and if necessary, replace the septum. Check the injection port liners. Check the tightness of the column nuts.

Every Week
Check the foreline pump oil level and diffusion pump fluid. Change the injection port liners and O-rings. Gas ballast the foreline pump.

GC and GC/MS

Every Month
Clean the split/splitless inlet vent line trap. Check for leaks (inlet and column connections).

Every Three Months


Replace gas cylinders (when below 500 psig).

Every Six Months


Replace the foreline pump oil. Check, and if necessary, refill the calibration vial.

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199

GC/MS Parts and Supplies


MSD Maintenance Schedule Every Year
Replace the diffusion pump fluid. Recondition or replace internal and external traps and chemical filters on the GC.

As Needed
Tune the MSD. Clean the ion source. Replace the carrier gas trap. Replace worn-out parts (filaments, EM, etc.). Replace the column. Lubricate seals.

Monitor
Record all tune values such as electron multiplier and ion source parameters in a log book to monitor instrument performance. In addition, note the high vacuum and foreline vacuum pressures.
Every week              Every 6 months Every year As needed 

Task Tune the MSD Change injection port liners Check the foreline pump oil level Gas ballast the foreline pump Check the calibration vial Replace the foreline pump oil Check the diffusion pump fluid Replace the diffusion pump fluid Replace the traps and filters Clean the ion source Change the carrier gas trap(s) and purifier Replace the worn out parts Lubricate seals (where appropriate) Replace column

GC and GC/MS

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GC/MS Parts and Supplies


MSD Contamination

Contamination
Contamination is usually identified by excessive background in the mass spectra. It can come from the GC or from the MSD. The source of the contamination can sometimes be determined by identifying the contaminants. Some contaminants are much more likely to originate in the GC, others are likely to originate in the MSD. Contamination sources originating in the MSD: Air leaks Cleaning solvents and materials Fingerprints inside the manifold Diffusion pump fluid Foreline pump oil

Air Leaks
Air leaks are a problem for any instrument that requires a vacuum to operate. Leaks are generally caused by vacuum seals that are damaged or not fastened correctly. Symptoms of leaks include: Higher than normal vacuum manifold pressure or foreline pressure Higher than normal background Peaks characteristic of air (m/z 18, 28, 32, and 44 or m/z 14 and 16) Poor sensitivity Low relative abundance of m/z 502 (this varies with the tune program and MSD used) Remedy Check interface nut for tightness. Replace if necessary. Check, and leak test the GC injection port. Helpful Hint: The most likely point for an air leak is a seal you recently opened.
Description MS interface column nut Column nut for GC/MS and two hole ferrules Universal column nut 2/pk Unit Part No. 05988-20066 05921-21170 5181-8830

GC and GC/MS

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201

GC/MS Parts and Supplies


MSD Contamination Cleaning Solvents
It is common to see cleaning solvent peaks in the mass spectra shortly after the ion source is cleaned. Remedy Dry all cleaned metal parts in the GC oven before reassembling and reinstalling them. Refer to specific cleaning procedures in your MSD Hardware Manual. Use a temperature above the boiling point of the solvent but below the limit of the column.

Fingerprints
Fingerprints contain hydrocarbons that can appear in mass spectra. Hydrocarbon contamination is characterized by a series of mass peaks 14 amu apart. The abundances of these peaks decrease as peak mass increases. Fingerprint contamination is usually caused by the failure to wear lint-free, nylon gloves during ion source cleaning, GC inlet maintenance, or from installing the column. Use special care to avoid recontamination of parts after you clean them. This typically occurs after some maintenance or part replacement. Remedy Reclean using clean, nylon gloves and proper cleaning techniques.
Description Nylon gloves, lint-free, large Nylon gloves, lint-free, small Part No. 8650-0030 8650-0029

GC and GC/MS

Diffusion Pump Fluid


If the diffusion pump is allowed to operate with no column (carrier gas) flow into the vacuum system, vapor from the diffusion pump fluid can drift up into the vacuum manifold. A more serious problem is when fluid is back streamed into the vacuum manifold by sudden or improper venting of the vacuum system. If a diffusion pump has back streamed, a prominent peak will often be seen at m/z 446 and the spectral baseline will exhibit increased background noise. Remedy If m/z 446 appears please call Agilent for assistance.

Foreline Pump Oil


Foreline pump oil contamination is characterized by peaks spaced 14 amu apart (hydrocarbons). Contamination with foreline pump oil is less common than contamination with diffusion pump fluid. Remedy Call Agilent for assistance.

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GC/MS Parts and Supplies


MSD Contamination Contamination Identification
The following table lists some of the more common contaminants, the ion characteristic of those contaminants, and the likely sources of those contaminants.
Ions (m/z) 13, 14, 15, 16 18, 28, 32, 44 or 14, 16 31, 51, 69, 100, 119, 131, 169, 181, 214, 219, 264, 376, 414, 426, 464, 502, 576, 614 31 43, 58 78 91, 92 105, 106 151, 153 69 73, 147, 207, 221, 281, 295, 355, 429 77, 94, 115, 141, 168, 170, 262, 354, 446 149 Compound Methane H2O, N2 , O2 , CO2, CO2 or N, O PFTBA and related ions Possible Source CI gas Residual air and water, air leaks, outgassing from Vespel ferrules PFTBA (tuning compound)

Methanol Acetone Benzene Toluene or xylene Xylene Trichloroethane Foreline pump fluid or PFTBA Dimethylpolysiloxane Diffusion pump fluid Plasticizer (phthalates)

Cleaning solvent Cleaning solvent Cleaning solvent Cleaning solvent Cleaning solvent Cleaning solvent Foreline pump oil vapor or calibration valve leak

GC and GC/MS

Septum bleed or methyl silicone column coating Diffusion pump fluid and related ions Vacuum seals (O-rings) damaged by high temperatures, use of vinyl or plastic gloves Fingerprints, foreline pump oil

Peaks spaced 14 amu apart

Hydrocarbons

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GC/MS Parts and Supplies


MSD Ion Source

Maintaining the Ion Source


Cleaning procedures for MSDs vary. Refer to your MSD Hardware Manual for specific ion source cleaning procedures. Common measures of instrument performance: Abundance of certain ions (e.g., percentage of the 502 ion from the Autotune report) Shape of lens ramps and the chosen voltages, especially Repeller Ramp Sensitivity obtainable for a given analysis Ability to tune to a given reference compound (e.g., DFTPP) When to clean: The ion source should be cleaned: According to a customers predefined schedule Based on instrument performance (e.g., deteriorated performance over time)
5973 Ion Source Assembly (EI)

Frequency of cleaning: The number of samples run (throughput) The type of samples Unique, established laboratory protocol Selecting a cleaning method: The primary action of any cleaning procedure is to remove contamination from surfaces. Removing this contamination restores the electrostatic properties of the ion source lensing system. Numerous cleaning methods have been developed for restoring ion source performance. The cleaning methods include abrasive, sonic, and electropolish. Abrasive methods offer several advantages: Provide adequate energy to remove contamination from surfaces Require minimal equipment Pose minimal risks to the user

GC and GC/MS

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GC/MS Parts and Supplies


MSD Ion Source
Cleaning and Maintenance Supplies
Description
Cleaning and Maintenance

Part No. 8650-0030 8650-0029 9310-4828 5181-8863


Cleaning and Maintenance Supplies

Nylon gloves, lint-free, Large Nylon gloves, lint-free, Small Lint-free industrial wipes, 100% cotton, 9 x 9 in. (300/pk) Generic ion source cleaning kit for all GC/MS types Includes: Cloths, lint-free (15/pk), Abrasive sheets (5/pk), Cotton swabs (100/pk), Nylon gloves, lint-free, Alumina powder, abrasive Cloths, lint-free (15/pk) Abrasive Sheets, aluminum oxide green lapping paper for ion source cleaning, 600 mesh (5 sheets) Alumina powder, abrasive PFTBA sample, certified (10 g) PFTBA sample kit, 0.5 mL Activated alumina, absorbent pellets for Edwards rough pump traps, non-LC/MS (1 lb can) PFTBA glass vial Cotton swabs (100/pk)
Tools

05980-60051 5061-5896 8660-0791 8500-0656 05971-60571 8500-1233 05980-20018 5080-5400

GC and GC/MS

Screwdriver, Pozidriv #1 pt, 3 in., fits no. 2 - 4 screws Screwdriver, Pozidriv #2 pt, 4 in., fits no. 5 - 10 screws Wrench, open-end, 1/4 x 5/16 in. Hex nut driver, 5.5 mm Screwdriver, TORX, T20 Screwdriver, TORX, T15 Screwdriver, TORX, T10 Ferrules and O-rings Teflon 1/4 in. ferrule (back) Teflon 1/4 in. ferrule (front) Retainer rings (10/pk)

8710-0899 8710-0900 8710-0510 8710-1220 8710-1615 8710-1622 5182-3466 0100-0160 0100-0787 5181-1258

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GC/MS Parts and Supplies


MSD Ion Source
Cleaning and Maintenance Supplies (continued)
Description 5973 One Year Maintenance Kit (for diffusion pump systems) Includes: Big Universal Trap for He (1/8 in.), Abrasive sheets (5/pk), Cloths, lint-free (15/pk), Cotton swabs (100/pk), SantoVac Ultra, 18.5 mL (2 each), Rough pump oil, 1 liter, Filament assembly, Octafluoronapthalene (OFN) MSD Tool Kit Includes: Small cleaning rod, Large cleaning rod, Source hold tool, Cotton swabs (100/pk), Nylon gloves, lint-free, Abrasive sheet, 30 mm (5/pk), Tool kit (wrenches, driving tools) Part No. 5183-2096

05971-60561

Helpful Hint: Details about specific MSD maintenance can be found in your models specific hardware manual.

MSD Ion Source Parts (EI) 5973


Item (A) (B) (C) Description Ion source body Repeller Interface socket Drawout plate Drawout cylinder Ion focus lens Entrance lens Repeller insulator Set screw Washer, M3 (5/pk) Nut, 5.5 mm Ion Source Sensor Lens insulator, (2/pk) Ion source assembly Source heater assembly Repeller assembly Screw (for filament on the source) 5973 Part No. G1099-20130 G1099-20132 G1099-20136 05971-20134 G1072-20008 05971-20143 05971-20126 G1099-20133 0515-1446 3050-0891 0535-0071 G1099-60104 05971-20130 G1099-60106 G1099-60177 G1099-60170 0515-1046

GC and GC/MS

C H M L

(D) (E) (F) (G) (H) (I) (J) (K) (L) (M)

F E D

5973 Ion Source Parts (EI)

B I I H K J L H

5973 Repeller Assembly (EI)

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GC/MS Parts and Supplies


MSD Ion Source/MSD Filaments
5972/5971/GCD MSD Ion Source Parts (EI)
Item (A) (B) (C) (D) (E) (F) (G) (H) (I) Description Entrance lens Lens insulator Ion focus lens Drawout cylinder Drawout plate Ion source body Set screw Repeller assembly Screw (for filament on the source) Ion source assembly (5972) Ion source assembly (5971/GCD) Transfer line tip, gold plated (5972/5971) Transfer line tip, gold plated (GCD) 2/pk Unit Part No. 05971-20126 05971-20130 05971-20143 G1072-20008 05971-20134 05971-20128 0515-1446 05971-60170 0515-1046 05971-60226 05971-60102 05971-20305 G1800-20305
5972/5971/GCD Ion Source (EI)

B A D F C B E G H

Helpful Hint: It is good practice to replace scratched lenses and other ion source parts. Scratched source parts lead to poor performance.

GC and GC/MS

Maintaining the Filaments


Like the filament in an incandescent light bulb, the ion source filaments will eventually burn out. Certain practices will reduce the chance of early failure: When setting up data acquisition parameters, set the solvent delay so that the analyzer will not turn on while the solvent peak is eluting. When the software prompts Override solvent delay at the beginning of a run always select No. Higher emission current will reduce filament life. If you are controlling your MSD from the Edit Parameters screen, always select MS Off before changing any of the filament parameters. Description Filament, 5973 (EI) Filament, 5973 (CI) Filament, 5972 (EI/CI) Filament, 5971 (EI/CI) / GCD (EI) 2/pk Unit Part No. 05971-60053 G1099-80053 05971-60053 05971-60140

5973/5972 Filament

Helpful Hint: It is very useful to switch from one filament to the other every three months so that when one filament fails, you know the other will fail soon. This will allow you to change both filaments at the same time.

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GC/MS Parts and Supplies


MSD Vacuum Systems and Pumps

Vacuum Systems and Pumps


A properly maintained vacuum system will: Prevent premature filament failure Provide better sensitivity Require less frequent source cleaning Extend quadrupole lifetime Prevent premature EM Horn failure Helpful Hint: Keeping a pan under the vacuum pump helps to detect and identify the origin of oil leaks.

Calibration
The calibration valve is an electromechanical valve with a vial for tuning compounds. Perfluorotributylamine (PFTBA) is the most commonly used tuning compound. It is required for automatic tuning of the MSD in EI mode. The tuning compound is usually a liquid but can be a volatile or semi-volatile solid.
Description PFTBA sample, certified PFDTD sample Unit 10 g 10 g Part No. 8500-0656 8500-8130

Note: If using the 5973 with a CI source, use Perfluorodimethyltrioxadodecane (PFDTD) sample.

GC and GC/MS

Diffusion Pump
It is not necessary to change the diffusion pump fluid more than once a year, unless you observe symptoms that suggest a problem with the diffusion pump fluid. The MSD must be vented in order to check the diffusion pump fluid (except for the 5973). Therefore, the best time to check the fluid is when the instrument is already vented for other maintenance. How to Check the Fluid Level: 1. If it is not vented already, shut down and vent the MSD according to instrument manual. 2. Unplug the MSD power cord. 3. Remove the pump and cover the top with aluminum foil. 4. After heating the pump in a GC oven at 60C for 15 minutes to make the fluid flow down into the reservoir at the bottom, remove the stack parts. 5. Inspect the pump fluid. If the fluid is discolored or contains particulate material, the fluid must be changed. 6a. Use a metal ruler to determine the depth of the fluid. A pump that has been in operation should have a pool 9 mm +/- 1 mm deep. Fluid in freshly charged pumps will be 12 mm deep. It is normal that up to 2 mL of oil may be in the rear portion of the vacuum manifold. The recommended total fluid charge for the 5971/5972 is 18 mL (+/- 2 mL). 6b. For the 5973 use the sight glass to determine the depth of the fluid. The recommended total fluid charge is approximately 37 mL. Helpful Hint: Use chemical-resistant gloves and safety glasses when replacing pump fluid. Avoid all contact with the fluid.

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GC/MS Parts and Supplies


MSD Vacuum Systems and Pumps
Description Diffusion pump fluid: SantoVac Ultra 5P, 18.5 mL (5973, 5972 or 5971/GCD) Ion gauge controller (5973/5972A) Ion gauge tube for measuring vacuum (5971/5972) Triode gauge tube for measuring vacuum (5972/5973) Part No. 6040-0809 59864B 0960-0376 0960-0897

Foreline Pump
The oil in the foreline or rough pump should be replaced on average once every six months, but can vary depending upon applications. After oil replacement, if a foreline trap is present, the molecular sieves should be replaced. Avoid contact with the pump oil. The residue from some samples may be toxic. Dispose of used oil properly.
Description Rough pump oil, 1 gal Inland 45 (5973, 5972 or 5971/GCD) Rough pump oil, 1 liter Inland 45 (5973, 5972 or 5971/GCD) Molecular sieve (5973, 5972 or 5971/GCD) Part No. 6040-0798 6040-0834 9301-1104

General Instructions on How to Replace the Pump Oil

GC and GC/MS

1. Vent and shut down the MSD. 2. Place a container under the drain plug on the foreline pump. 3. Remove the fill cap from the top of the pump to expose the fill hole. 4. Remove the drain plug from the pump. 5. Reconnect the MSD to its power source. Switch on for 2 or 3 seconds, and then switch it off again. This displaces old oil from the internal pump cavities. Disconnect the power cord again. 6. Reinstall the drain plug and pour pump oil into the fill hole. 7. Reinstall the fill cap. 8. Reconnect the MSD power cord. 9. Start up and pump down the MSD according to the Instrument Manual procedure. Note: Subtle differences may exist between MSD models. Consult your hardware manual for specific instruction.

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GC/MS Parts and Supplies


Electron Multipliers and Replacement Horn

Electron Multipliers and Replacement Horn


Maximize Lifetime
The lifetime of an EM is directly related to the current that flows through it and the extent of contamination or condensation that it experiences. To maximize electron multiplier life: Maintain the best possible vacuum, especially in the analyzer manifold. Use extreme caution and be conservative with venting, pumpdown, and all vacuum system procedures to keep pump fluid background to a minimum. After venting, allow four hours for pump-down and thermal equilibration before scanning. Actively look for background contamination and leaks and repair them immediately. Dont tune excessively. PFTBA can result in higher background over an extended period of time. Symptom Voltage is over 2500 volts Poor vacuum Remedy Replace electron multiplier or horn
Description Electron multiplier replacement horn (5973, 5972, 5971, GCD) High energy dynode (5973 only) Part No. 05971-80103 G1099-80001 05971-80102

Electron Multiplier Replacement Horn

GC and GC/MS

Electron multiplier kit (5971 & 5972 only)

Note: These are the recommended replacement multipliers and horns for the MSD. Substituting other manufacturers products can result in reduced sensitivity, lifetime, and noise problems.

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GC/MS Parts and Supplies


MSD NoVents

MSD NoVents
A Timesaving Solution
Agilent Technologies' MSD NoVents allow users to turn on the purge gas and change the column or service the inlet or column without venting. No air or water enters the system. The MSD remains stable and is operational in minutes. Advantages of Agilent's MSD NoVents No oxygen or air in your system during inlet maintenance, prolonging column life A constantly stable MSD, saving hours with each column change or inlet maintenance No MSD or interface temperature cycling, minimizing the possibility of leaks A cleaner ion source, with less downtime for servicing Convenient accessibility in the GC oven Proven fitting design for easy column removal or installation Simple operation, perfect for inexperienced users MSD NoVents are available in two different kits that include all the necessary tools, fittings and instructions: For Agilent 6890/5973/5972 GC/MSD. Designed for seamless integration with the Agilent 6890 GC system. Available exclusively from Agilent. For Agilent 5890/5972/5971 and GCD. Both MSD NoVent kits include a helium purge gas control module that, for the Agilent 6890 MSD NoVent, fits into one of the GC detector slots and is controlled by the Agilent 6890 electronics. For the Agilent 5890 MSD NoVent, the module sits externally and is controlled manually. Both setups require a specific restrictor tube that acts as the transfer line from the GC to the MS source.

GC and GC/MS

MSD NoVent Tee

The MSD NoVent uses a unique fluidic valve design and deactivated fused silica interface tube that mounts directly on the MS interface inside the GC oven. A soft brass threaded insert connects to the MS interface, providing no interface damage. This design ensures that a helium purge maintains a positive flow from the valve to the atmosphere when a column is disconnected, preventing air or water from entering the MS.

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GC/MS Parts and Supplies


MSD NoVents/8270 SemiVolatiles Applications Kit
MSD NoVents
Description 6890 MSD NoVent Includes two each restrictors for 5973 and 5972.
6890 MSD NoVent

Part No. 5183-4782 5183-4783

5890 MSD NoVent Restrictors not included and must be ordered separately.

MSD NoVent Replacement Parts


Description MSD NoVent Restrictors ID (mm) Unit Part No.

For use with 5971/5972 For use with 5973 For use with GCD
SilTite Metal Ferrules

2/pk 2/pk 2/pk

5183-4785 5183-4786 5183-4787

For use with 0.20-0.25 mm id capillary columns. Includes 2 column nuts For use with 0.32 mm id capillary columns. Includes 2 column nuts For use with 1/16 in. od SS tubing. Includes 2 column nuts Interface Tee

0.4 0.5

10/pk 10/pk 10/pk

5184-3569 5184-3570 5184-3571 5185-5800

GC and GC/MS

G2860A 8270 Semi-Volatiles Applications Kit


The G2860A 8270 Semi-Volatiles Applications Kit is designed for use in Agilent 6890/5973A and 6890/5973N GC/MSD Systems. The kit provides modified and/or pretested components to improve system performance for USEPA Method 8270. With the kit, system linearity is maximized and activity is minimized. Contents: Start-Up Guide, pub. no. 5988-3073EN Application Note, Improvements in the Agilent 6890/5973 GC/MSD System for use with USEPA Method 8270, pub. no. 5988-3072EN Ultra Ion Source Chamber Ultra Repeller Ultra Large Aperture Drawout Plate Pre-tested column, 30 m x 250 m x 0.5 m HP5 MS, P/N 19091S-139 Single-taper splitless liner, 4 mm id, deactivated, P/N 5181-3316 Direct Connect Liner, single taper, 4 mm id, deactivated, P/N G1544-80730 Direct Connect Liner, dual taper, 4 mm id, deactivated, P/N G1544-80700 Floppy Disk with tuning macros
Description 8270 Semi-volatiles Application Kit Part No. G2860A

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GC/MS Parts and Supplies


MS Engine Supplies

Maintaining the MS Engine


Maintenance Schedule
Common maintenance tasks are listed in the following maintenance schedule table. Performing these tasks on a regular basis can reduce overall operating costs. Keep a record (logbook) of system performance characteristics and maintenance operations performed. This makes it easier to detect variances from normal operation and to take corrective action.

Maintenance Schedule
Task Autotune or manual tune (save results)
Cabinet Maintenance

Every Week

Every 3 Months

Every 6 Months

As Needed


Clean the cabinet Inspect hoses and cords Vacuum the fan filter
Vacuum System Maintenance

  

Check mech. pump oil Replace mech. pump oil Replace mech. pump traps Check diffusion pump fluid Degas ion gauge tube Replace ion gauge tube Replace seals & O-rings
Analyzer Maintenance

      

GC and GC/MS

Clean ion source Replace filament Replace ion source heater Replace mass filter heater Replace electron multiplier horn
GC/MS Interface Maintenance

    

Refill EI calibration vial Refill CI calibration vial Replace interface heater

  

Helpful Hint: We recommend keeping a separate set of tools that have been thoroughly cleaned for working with the MS engine. These tools are needed to prevent contamination when reassembling or installing clean assemblies such as the ion source.

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GC/MS Parts and Supplies


MS Engine Supplies Cleaning the Ion Source
There is not a regular interval for ion source cleaning. The ion source should be cleaned when symptoms indicate. Symptoms of a dirty ion source include poor sensitivity and inadequate abundances at high masses. See the Troubleshooting chapter in your hardware manual for more information about these symptoms. Many of the tools and supplies needed to service the 5989 MS Engine are included in the installation kit supplied with the instrument. The following table lists common cleaning supplies and consumable parts used in the maintenance of the MS Engine.

Cleaning and Maintenance


Description Abrasive paper, 30 mm Alumina powder, abrasive Cloths, lint-free Lint-free industrial wipes, 100% cotton, 9 x 9 in. Cotton swabs Nylon gloves, lint-free Grease, high-vacuum PFTBA sample, certified PFTBA sample kit 15/pk 300/pk 100/pk 1 pair 10 g 0.5 mL Unit 5/pk Part No. 5061-5896 8660-0791 05980-60051 9310-4828 5080-5400 8650-0030 6040-0289 8500-0656 05971-60571

GC and GC/MS

5989 MS EngineIon Source Parts and Supplies


Item (A) (B)
5989 Lens Stack

Description Clamp insulator, ion source Entrance lens Lens insulator, ion source Plate insulator, ion source EI/CI repeller Repeller insulator Filament block Filament assembly Heater cartridge

Part No. 05989-20110 05989-67002 05989-20111 05989-20109 05989-20145 05989-20119 05989-20165 05985-60179 05989-60098

(C) (D) (E) (F)

5989 Repeller Assembly

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GC/MS Parts and Supplies


MS Engine Supplies
MS Engine Pump Lubricants and Oils
Lubricant Oil Turbo pump lubricant: 5989 or 5988 Thermo or Electro Spray 5985 or 5987 5999x, 5993x or 5995x Part No.

Balzers turbo pump lubricant, 0.25 liter


Diffusion pump oil:

6040-0468

SantoVac 5, 1 liter SantoVac 5, 128 mL


Rough pump oil:

 

 

 

6040-0370 6040-0819

Rough pump oil, 1 gal Inland 45 Rough pump oil, 1 liter Inland 45 Particle Beam pump oil, Fomblin

  

 

 

 

6040-0798 6040-0834 6040-0730

Replacement Parts and Supplies


The electron multiplier lifetime on the 5989 may be 6 to 9 months or shorter, especially with the following applications: Thermospray Particle Beam Purge and trap, with no jet separator Extensive CI High sensitivity work with high EM voltage
Description
5989

GC and GC/MS

Unit

Part No. 05989-80043 05971-80103

Electron multiplier kit Electron multiplier replacement horn


Parts and Supplies

Ferrule Ion gauge tube, K-25 flange


O-rings and Seals

2/pk

5181-3366 0960-0799

Insulating ring O-ring, detector flange Diffusion pump fluid fitting fill and drain cap O-ring KF 25 centering ring and O-ring O-ring, manifold window 5/pk 12/pk

05989-20705 5181-3367 0905-1145 3162-0110 0905-1189

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GC/MS Parts and Supplies


MS Test and Performance Samples

MS Test and Performance Samples


Each GC/MS has a specific test and performance sample. Refer to the chart below for the exact sample.
Tuning Samples Performance Verification Samples El Cl MSD Tune Tune 5973 PFTBA PFDTD 5972 PFTBA PFTBA 5971 PFTBA PFTBA GCD PFTBA NA El OFN 1pg/L HCB 10 pg/L HCB 10 pg/L Sample A 10 ng/L Negative Mode Cl OFN 1pg/L NA NA NA Positive Mode Cl Checkout Samples SemiVolatile Volatile BFB BFB BFB BFB

Benzophenone DFTPP 100 pg/L Benzophenone DFTPP 100 pg/L Benzophenone DFTPP 100 pg/L NA DFTPP

MS Engine

5989A PFTBA PFTBA 5989B PFTBA PFTBA

HCB 50 pg/L HCB 20 pg/L

OFN 1 pg/L OFN 500 fg/L

Benzophenone DFTPP 100 pg/L Benzophenone DFTPP 100 pg/L Part No. 05970-60045

BFB BFB

GC and GC/MS

Description Evaluation Sample for GC/MS Systems Contains 6 vials: 4 vials (Sample A, 10 ng/L), 1 vial (Sample B, 100 pg/L), and 1 vial (Sample C, 100 ng/L) each of dodecane biphenyl, p-chlorodiphenyl, and methyl palmitate in isooctane, 1 mL ampoules GC/MS Tuning Standard contains: DFTPP, Benzidine, Pentachlorophenol, and p,p-DDT 1 mg/mL in methylene chloride 5989 Installation Sample Kit contains: HCB 50 pg/L, HCB 20 pg/mL, Benzophenone 100 pg/L, OFN 1 pg/L, mix of HCB, OFN and Benzophenone 5 ng/L Extended Mass Performance Sample, 19 g, fomblin oil PFTBA certified, 1 bottle, 10 g PFTBA Sample Kit, 0.5 mL PFDTD, 10 g Benzophenone, 100 pg/L, 5 ampoules Hexachlorobenzene, 10 pg/L, 4 ampoules 1 ng/L, 2 ampoules Hexachlorobenzene, 20 pg/L Methyl stearate (in methanol); 1 ng/L, 2 ea PFTBA not certified Octafluoronapthalene (OFN), 1 pg/L, 5 ampoules Octafluoronapthalene (OFN), 500 fg/L p-Bromofluorobenzene (BFB), 25 g/mL

8500-5995

8500-6406

8500-5500 8500-0656 05971-60571 8500-8130 8500-5440 8500-5808 8500-6405 05990-60075 8500-5441 8500-6572 8500-5851 www.agilent.com/chem

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GC Columns The J&W Scientific Brand of Technically Advanced GC Columns


In 2000 Agilent Technologies merged with J&W Scientific. The worlds two largest column manufacturers combined to become one. HP- and DB- are both dependable GC column names that you have relied upon for over 25 years. Now you can find both in one box, under one brand nameJ&W Scientificfrom one company, Agilent Technologies. The box is new, but the quality production processes and your results will remain the same. Your J&W Scientific columns are backed by decades of chromatography experience and column manufacturing know-how. This enables us to provide superior-quality, dependable GC columns and unmatched service and support. The DB- and HP- capillary column product lines and the manufacturing operations have been consolidated in Folsom, California, USA. As always, every GC column meets the tighest quality control specifications in the industry. So if youre worried about the consistency of your next HP-5ms, DB-1, or any other column, rest assured that you will be getting the same GC columns youve been relying on for years. Our goal is to continually refine our materials and processes to provide the best columns in the world. And each one will continue to deliver the high quality and superior performance you have come to expect.

GC and GC/MS

With the Industrys Best Low-bleed Columns


Agilent invests heavily in R&D to develop superior-performing, low-bleed GC columns, gaining us respect industrywide as the low-bleed leader.

Wed rather be known for no bleed.


Which is precisely why were obsessed with reducing column bleed to the bare minimum and developing new stationary phase chemistries. Why all the concern about column bleed, you ask? Because column bleed is the degradation of the stationary-phase polymer that accelerates at a higher temperature, elevating the baseline as the temperature rises to the columns upper thermal limit. Ultimately, the signal-to-noise ratio dictates analytical sensitivities and detection limits. Decrease the noise and the sensitivity will increase, which is a good thing. Get rid of column bleed and you help eliminate the noise that can decrease MS spectral integrity, reduce instrument uptime, decrease column lifetime and lower reproducibility at trace levels.

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GC Columns
Why low-bleed columns are a smart choice.
Standard 35%Phenyl

If you perform trace analyses where sensitivity and instrument performance are important, column bleed (or the lack thereof) should be important to you. Many GC detectors are sensitive to contamination from bleed. By using low-bleed columns, these detectors require less maintenance. Due to the unique relationship between ion storage capacity of the trap and sensitivity levels, ion trap MS users will especially benefit from low-bleed columns. J&W brands low-bleed GC columns also give you the advantages of superior inertness and increased upper temperature limits.

DB-35ms
10 12 14 16
Time (min)

What makes J&W Scientific low-bleed columns so special?


20 22

18

Primary Quant Ion is hidden

276

Standard 35% Phenyl

GC and GC/MS

In short, its J&Ws unique polymer chemistry and proprietary deactivation. Our MS columns utilize special surface deactivation and siloxane chemistries that enhance the chromatographic performance of siloxane polymers. To provide selectivities virtually identical to their conventional counterparts, we offer DB-5ms, DB-35ms, DB-17ms and DB-225ms. The HP-5ms uses the same stationary phase as the standard HP-5, providing low bleed without the use of arylene technology. DB-1ms and HP-1ms provides the same selectivity as the standard 100% dimethylpolysiloxane (DB-1 type) column. Plus, our unique DB-1ms polymer chemistry and proprietary deactivation techniques help us increase temperature limits (340/360), improving thermal stability and optimizing performance. Because the polysiloxane backbone of the stationary phase is optimized to yield the lowest-bleed column possible, DB-XLB doesnt have any conventional counterparts. You can gain sensitivity by replacing your general-purpose non-polar columns, such as 100%-dimethylpolysiloxane and 5%-diphenyl/95% dimethylpolysiloxane, with DB-XLB.

276

Primary Quant Ion is enhanced

With the Tightest QC Specs in the Business!


DB-35ms

One nanogram of benzo[g,h,i]perylene (m/z 276) at 340C on a standard 35%-phenyl column and on DB 35ms. The increased signal-to-noise ratio is apparent in the chromatograms (top), and the cleaner spectra (lower) will result in more confident peak identifications.

We make more capillary GC columns than anyone else in the world. And we still build them with our consistently rigid performance criteria, such as separation efficiency, retention characteristics, peak height ratios and bleed rates.

Better precision. For better results.


J&W Scientific columns adhere to very tight retention factor (k) specifications, helping ensure consistent retention from column to column. To assure separation reproducibility for the widest range of compounds, we measure at least three retention indices for each column. Narrow retention index ranges help ensure reproducible peak separations from column to column, while a wide range indicates a failure to precisely control stationary phase structure.

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GC Columns
Higher performance. And nice and narrow peaks.
Theoretical plates per meter measures column efficiency (N). Columns with higher plate numbers generate the narrowest peaks, improving the resolution of closely eluting peaks. Paying close attention to the surface deactivation process and coating efficiency of the stationary phase is critical to obtain the highest number of theoretical plates.

Increased sensitivity. With very low bleed levels.


Bleed is the columns contribution to the background signal. The lowest-bleed columns provide the best analytical sensitivity, which is why we devote substantial research to reduce column bleed. We work hard to create the widest range of low-bleed stationary phases. Weve also improved our standard phase columns, resulting in even lower bleed levels.

Excellent peak shape.


Agilents stringent QC performance evaluation tests provide the utmost confidence in the qualitative and quantitative results, especially for difficult to chromatograph compounds. We measure peak height ratios for acidic and basic compounds to ensure the highest column performance for the widest range and types of compounds. We also carefully monitor peak symmetry and tailing for a broad range of chemically active compounds.

GC and GC/MS

What Do Our Tighter Performance Criteria Do For You?


Feature Narrow Retention Index and Retention Factor Window Increased Plates per Meter Lowest Bleed Advantage Highest level of column-to-column reproducibility Minimal method adjustment when changing columns Highest level of resolving power Improved sample throughput Increased analytical sensitivity for all detectors Fast baseline stabilization Faster column exchange Excellent thermal stability Benefit Confidence in analytical results Improved sample throughput, reduced downtime Accurate quantification Potentially shorter run times Improved detection limits Reliable compound identification Minimized conditioning time Increased sample throughput Increased column lifetime, reduced downtime Increased sample throughput

Specify J&W chemistry for intra-company methodologies Confidence in method transfer and intra-company results

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219

GC Columns
Choosing a Column

Choosing a Capillary GC Column


Selecting the best capillary column for an analysis can be an uncertain and sometimes difficult task. The first step should always be to refer to what has already been done. Our chemists have put together a variety of resources to help you find the right column for your analysis. Take a look at:

Chromatograms
Our chemists continue to evaluate and develop methods for a multitude of applications. Weve put together some of the more common chromatograms with column recommendations and method parameters for your reference in this catalog (pages 347441). If you dont find what youre looking for, take a look on our website for a more extensive chromatogram library and a compound search function (go to www.agilent.com/chem, then click on Library).

Method Guides
Weve evaluated the most common Environmental/EPA methods, ASTM methods, and general compound analyses and have put together simple guides which specify the best column recommendation for these methods. These are available for your reference on pages 315323.

Retention Data Lists

GC and GC/MS

Take a look at our solvent and pesticide retention data lists (pages 341347). Weve analyzed hundreds of compounds on several different phases to help you determine which column will be the best choice for your list of compounds.

Reference Section
If you are still unable to find an existing method for your application, take a look at our reference section on pages 324340. While there are no foolproof techniques, shortcuts, tricks or secrets to column selection, there are some guidelines. Our reference guide gives you helpful hints for choosing a stationary phase, picking the right column dimensions, developing temperature programs and determining the right inlet and detector for the application.

Contact Us
Even though weve developed many reference tools to help you find the right column, we are always ready and happy to give the best column recommendation and method development advice possible. Our Technical Support Chemists have over a 100 years of combined experience running samples and developing methods. We are the gas chromatography column experts and we are at your disposal! Send in questions on the Technical Support question form on our website www.agilent.com/chem or contact your local Agilent office.

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GC Columns
Columns for GC/MS

Columns for GC/MS


There is a rapidly increasing population of benchtop GC/MS instruments in analytical laboratories that analyze a broadening range of trace level, higher temperature samples. These samples require increasingly inert, lower bleed, higher temperature columns. In response to this growing need, Agilent Technologies deliberately designed several ms columns to chromatograph a broader range of low level samples and generating lower bleed even at higher temperatures. What makes a J&W low bleed column from Agilent unique? Unique polymer chemistry and proprietary surface deactivation, both of which have contributed to columns that adhere to the tightest quality control specifications in the industry for bleed, inertness, selectivity and efficiency. J&W ms columns utilize special surface deactivation and siloxane chemistries which enhance the chromatographic performance of siloxane polymers.

DB-1ms
100% Dimethylpolysiloxane, identical selectivity to DB-1 Non-polar Very low bleed characteristics, ideal for GC/MS Improved acid performance compared to standard 100% Dimethylpolysiloxane columns Improved signal-to-noise ratio for better sensitivity and mass spectral integrity 340/360C upper temperature limit Excellent general purpose column Bonded and cross-linked Solvent rinsable Test mix available. See pages 9697 for test mix information. Similar Phases: HP-1ms, Rtx-1ms, CP-Sil 5 CB Low Bleed/MS, MDN-1, AT-1
CH O Si CH
3 3

100%

GC and GC/MS

Structure of Dimethylpolysiloxane

DB-1ms Chromatograms
Drug Screen page 416

DB-1ms
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.10 0.10 0.10 0.10 0.20 0.20

10 10 20 20 12 25

0.10 0.40 0.10 0.40 0.33 0.33

-60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360

127-0112 127-0113 127-0122 127-0123 128-0112 128-0122

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GC Columns
Columns for GC/MS
DB-1ms (continued)
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32

15 30 30 60 15 30 30 60

0.25 0.10 0.25 0.25 0.25 0.10 0.25 0.25

-60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360

122-0112 122-0131 122-0132 122-0162 123-0112 123-0131 123-0132 123-0162

HP-1ms
CH O Si CH
3 3

100%

Structure of Dimethylpolysiloxane

GC and GC/MS

100% Dimethylpolysiloxane Identical selectivity to HP-1 Non-polar Low bleed characteristics Excellent general purpose column Improved signal-to-noise ratio for better sensitivity and mass spectral integrity Bonded and cross-linked Solvent rinsable Similar Phases: HP-1ms, Rtx-1ms, CP-Sil 5 CB Low Bleed/MS, MDN-1, AT-1

HP-1ms
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.20 0.25 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.32

25 15 30 30 30 30 60 15 25 30 30 30 60

0.33 0.25 0.10 0.25 0.50 1.00 0.25 0.25 0.52 0.10 0.25 1.00 0.25

-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350

19091S-602 19091S-931 19091S-833 19091S-933 19091S-633 19091S-733 19091S-936 19091S-911 19091S-612 19091S-813 19091S-913 19091S-713 19091S-916

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GC Columns
Columns for GC/MS

DB-5ms
Phenyl Arylene polymer virtually equivalent to a (5%-Phenyl)-methylpolysiloxane Non-polar Very low bleed characteristics, ideal for GC/MS Excellent inertness for active compounds Improved signal-to-noise ratio for better sensitivity and mass spectral integrity Bonded and cross-linked Solvent rinsable Ion trap testing and certification available Exact replacement of HP-5TA Close equivalent to USP Phase G27 Test mix available. See pages 9697 for test mix information. Similar Phases: Rtx-5ms, PTE-5, CP-Sil 8 CB Low Bleed/MS, BPX-5, AT-5ms
CH O Si CH
3 3

CH Si CH

CH O Si CH

3 m

Structure of poly(dimethylsiloxy)poly(1,4-bis(dimethylsiloxy) phenylene)siloxane

DB-5ms Chromatograms
50 ppb Formaldehyde page 380 Amines and Nitriles page 397 Diesel Fuel page 348 EPA Air Analysis Compendium page 379 Method TO-15 (1 ppV Standard) EPA Method 525.2 page 367 EPA Method 551.1 page 377 EPA Method 8061 page 368 (Phthalate Esters) Narcotics page 420 Organochlorine Pesticides page 350 (EPA Method 8081A) Organophosphorous Pesticides page 356 (EPA Method 8141 A) Phenols pages 369, 412 Polyethyleneamines page 396 Substituted Anilines page 411 Sulfur in Air page 380

GC and GC/MS

DB-5ms
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.18 0.18 0.20 0.20 0.20 0.25 0.25 0.25 0.25 0.25 0.25

20 40 12 25 50 15 15 15 15 25 25

0.18 0.18 0.33 0.33 0.33 0.10 0.25 0.50 1.00 0.25 0.40

-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350

121-5522 121-5542 128-5512 128-5522 128-5552 122-5511 122-5512 122-5516 122-5513 122-5522 122-552A

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223

GC Columns
Columns for GC/MS
DB-5ms (continued)
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32

30 30 30 30 50 60 60 60 15 15 15 25 30 30 30 30 50 50 60 60 60 60 15 15 15 30 30 30

0.10 0.25 0.50 1.00 0.25 0.10 0.25 1.00 0.10 0.25 1.00 0.52 0.10 0.25 0.50 1.00 0.25 1.00 0.10 0.25 0.50 1.00 0.50 1.00 1.50 0.50 1.00 1.50

-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 300/320 -60 to 300/320 -60 to 300/320 -60 to 300/320 -60 to 300/320 -60 to 300/320

122-5531 122-5532 122-5536 122-5533 122-5552 122-5561 122-5562 122-5563 123-5511 123-5512 123-5513 123-5526 123-5531 123-5532 123-5536 123-5533 123-5552 123-5553 123-5561 123-5562 123-5566 123-5563 125-5517 125-551J 125-5512 125-5537 125-553J 125-5532

GC and GC/MS

0.32 0.32 0.32 0.32 0.32 0.32 0.53 0.53 0.53 0.53 0.53 0.53

DB-5ms.ITD
0.25 30 0.25 -60 to 325/350 122-4132

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GC Columns
Columns for GC/MS

HP-5ms
(5%-Phenyl)-methylpolysiloxane Identical selectivity to HP-5 Non-polar Very low bleed characteristics, ideal for GC/MS Excellent inertness for active compounds including acidic and basic compounds Improved signal-to-noise ratio for better sensitivity and mass spectral integrity Bonded and cross-linked Solvent rinsable Equivalent to USP Phase G27 Similar Phases: Rtx-5MS, Rtx-5 Amine, DB-5ms, PTE-5, CP-Sil 8CB Low Bleed/MS, BPX-5
CH 3 O Si CH 3
95%

C6H5 O Si C6 H5
5%

Structure of Diphenyldimethylpolysiloxane

HP-5ms Chromatograms
Chlorinated Pesticides: EPA page 352 Method 508 Nitrogen/Phosphorus Containing page 356 Pesticides: EPA Method 507 Organohalide Pesticides in page 352 Water, EPA Method 505 Phenols on HP-5MS page 412 USEPA Method 8270 page 366 Semivolatile Compounds

GC and GC/MS

HP-5ms
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.20 0.20 0.20 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25

12 25 50 15 15 15 30 30 30 30 60 60

0.33 0.33 0.33 0.10 0.25 1.00 0.10 0.25 0.50 1.00 0.10 0.25

-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350

19091S-101 19091S-102 19091S-105 19091S-331 19091S-431 19091S-231 19091S-333 19091S-433 19091S-133 19091S-233 19091S-336 19091S-436

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GC Columns
Columns for GC/MS
HP-5ms (continued)
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32

15 15 15 25 30 30 30 30 60 60

0.10 0.25 1.00 0.52 0.10 0.25 0.50 1.00 0.10 0.25

-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350

19091S-311 19091S-411 19091S-211 19091S-112 19091S-313 19091S-413 19091S-113 19091S-213 19091S-316 19091S-416

DB-XLB
EXceptionally Low Bleed Low polarity Extended temperature limit of 340/360C Unique selectivity Excellent inertness for active compounds Ideal for confirmational analyses Excellent for pesticides, herbicides, PCBs and PAHs Ideal for GC/MS Ion trap testing and certification available Bonded and cross-linked Solvent rinsable Test mix available. See pages 9697 for test mix information. Similar Phase: MDN-12 Note: DB-XLB is designed for inhibiting column bleed at high temperatures. It also appears to have inadvertently inherited an exceptional ability for separating many PCB congeners when used with MS detection. This stellar performance was maximized after careful optimization of the column dimensions, temperature programs, and carrier gas flow conditions... (Frame, G. Analytical Chemistry News & Features, Aug. 1, 1997, 468A-475A)

GC and GC/MS

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GC Columns
Columns for GC/MS
DB-XLB Chromatograms
Aroclors 1016-1268 (without 1221) CLP Pesticides PCB Congeners in DIN Method EPA 508.1 Pesticides EPA 8151A Phenoxy Acid Herbicides - Methyl Derivatives page 361 page 349 page 362 page 351 page 357 EPA 552.2 Garlic Oil Herbicides PCBs by EPA Method 8082 Phenols Ylang Ylang Oil page 370 page 384 page 358 page 363 page 369 page 386

DB-XLB
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.18 0.18 0.18 0.20 0.20 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.45 0.53 0.53

20 30 60 12 25 15 15 15 30 30 30 30 60 15 15 30 30 30 30 60 30 15 30

0.18 0.18 0.18 0.33 0.33 0.10 0.25 1.00 0.10 0.25 0.50 1.00 0.25 0.25 1.00 0.10 0.25 0.50 1.00 0.25 1.27 1.50 1.50

30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 320/340 30 to 320/340 30 to 320/340

121-1222 121-1232 121-1262 128-1212 128-1222 122-1211 122-1212 122-1213 122-1231

GC and GC/MS

122-1232 122-1236 122-1233 122-1262 123-1212 123-1213 123-1231 123-1232 123-1236 123-1233 123-1262 124-1232* 125-1212 125-1232

*No special ferrules required for high speed 0.45 mm id columns

DB-XLB.ITD
.25 30 .25 30 to 340/360 122-4232

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227

GC Columns
Columns for GC/MS

DB-35ms
Virtually equivalent to a (35%-Phenyl)-methylpolysiloxane Midpolarity Very low bleed characteristics, ideal for GC/MS Extended temperature limit of 340/360C Excellent inertness for active compounds Ideal for confirmational analyses Bonded and cross-linked Solvent rinsable Replaces HP-35ms Close equivalent to USP Phase G42 Test mix available. See pages 96-97 for test mix information. Similar Phases: Rtx-35, SPB-35, AT-35, Sup-Herb, MDN-35, BPX-35

DB-35ms Chromatograms
Anilines Barbiturates Benzodiazepines Chlordane CLP Pesticides EPA 508.1 Pesticides EPA 552.2 EPA 8151A Phenoxy Acid Herbicides - Methyl Derivatives page 411 page 418 page 419 page 364 page 349 page 351 page 370 page 357 Organochlorine Pesticides (EPA Method 8081A) Organophosphorous Pesticides (EPA Method 8141 A) PCBs by EPA Method 8082 Toxaphene page 350 page 357 page 363 page 364

GC and GC/MS

DB-35ms
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.20 0.20 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.53 0.53 0.53

15 25 15 30 30 60 15 30 60 15 30 30

0.33 0.33 0.25 0.15 0.25 0.25 0.25 0.25 0.25 1.00 0.50 1.00

50 to 340/360 50 to 340/360 50 to 340/360 50 to 340/360 50 to 340/360 50 to 340/360 50 to 340/360 50 to 340/360 50 to 340/360 50 to 320/340 50 to 320/340 50 to 320/340

128-3812 128-3822 122-3812 122-3831 122-3832 122-3862 123-3812 123-3832 123-3862 125-3812 125-3837 125-3832

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GC Columns
Columns for GC/MS

DB-17ms
Virtually equivalent to (50%-Phenyl)-methylpolysiloxane 320/340C upper temperature limit Very low bleed midpolarity column, ideal for GC/MS Excellent inertness for active compounds Enhanced mass spectral integrity Bonded and cross-linked Solvent rinsable Best column for CLP pesticides Test mix available. See pages 9697 for test mix information. Similar Phases: HP-50+, Rtx-50, 007-17, SP-2250, SPB-50, BPX-50, SPB-17, AT-50

DB-17ms Chromatograms
Hallucinogens PAHs Tocopherols page 420 page 368 page 419

DB-17ms
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.18 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32

20 15 15 30 30 60 15 15 30 30

0.18 0.15 0.25 0.15 0.25 0.25 0.15 0.25 0.15 0.25

40 to 320/340 40 to 320/340 40 to 320/340 40 to 320/340 40 to 320/340 40 to 320/340 40 to 320/340 40 to 320/340 40 to 320/340 40 to 320/340

121-4722

GC and GC/MS

122-4711 122-4712 122-4731 122-4732 122-4762 123-4711 123-4712 123-4731 123-4732

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229

GC Columns
Columns for GC/MS

DB-225ms
Virtually equivalent to (50%-Cyanopropylphenyl)-methylpolysiloxane Mid/high polarity Excellent for separations of cis- and trans-fatty acid methyl esters (FAMEs) Low bleed Bonded and cross-linked Solvent rinsable Close equivalent to USP Phase G7 Similar Phases: HP-225, SP-2330, CP-Sil 43 CB, Rtx-225, BP-225, OV-225, 007-225, AT-225

DB-225ms Chromatograms
Tetrachlorodibenzo-p-furans page 359

DB-225ms
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.25 0.25 0.25 0.32 0.32

15 30 60 15 30

0.25 0.25 0.25 0.25 0.25

40 to 240 40 to 240 40 to 240 40 to 240 40 to 240

122-2912 122-2932 122-2962 123-2912 123-2932

GC and GC/MS

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GC Columns
Polysiloxane Polymers

General Purpose Stationary Phases


Agilent makes more capillary columns than anyone else in the world and our commitment to product reliability and repeatability has raised the level of quality that chromatographers demand. We still continue to tighten our performance criteria such as separation efficiency, retention characteristics, peak height ratios and bleed rates. Our vast selection of high quality products is sure to meet the chromatographic needs for virtually any application.

Polysiloxane Polymers
Polysiloxanes are the most common stationary phases. They are available in the greatest variety and are stable, robust and versatile. Standard polysiloxanes are characterized by the repeating siloxane backbone. Each silicon atom contains two functional groups. The type and amount of the groups distinguish each stationary phase and its properties. Youll also find our GC/MS phases listed in this section. While some of the GC/MS phases utilize different polymer chemistries, their selectivities mimic the standard polysiloxane phases and offer the advantage of low bleed and in some cases extended temperature ranges. With the merger of Agilent and J&W Scientific there were many similar columns with the same type of polymer. In some cases the manufacturing and QC processes were exactly the same. In these cases the DB version was kept. In the cases where the HP and the DB columns had any manufacturing or QC differences, we opted to keep both phases available as in the case of DB-1 and HP-1. Each of these columns is a high quality product made to meet exacting quality control testing. However there may be some subtle performance differences. For example the DB-35 and the HP-35 have slightly different selectivities. Therefore, we are still offering both the DB and the HP version for our customers who have methods already developed on these columns.
R O Si R
n

R=CH3 CH2CH2CH2CN CH2CH2CF3

methyl cyanopropyl trifluoropropyl phenyl


Polysiloxanes

GC and GC/MS

DB-1
100% Dimethylpolysiloxane Non-polar Excellent general purpose column Wide range of applications Low bleed High temperature limit Bonded and cross-linked Solvent rinsable Wide range of column dimensions available Equivalent to USP Phase G2 Test mix available. See pages 9697 for test mix information. Similar Phases: HP-1, Ultra-1, SPB-1, CP-Sil 5 CB Low Bleed/MS, Rtx-1, BP-1, OV-1, OV-101, 007-1(MS), SP-2100, SE-30, CP-Sil 5 CB MS, ZB-1, AT-1, MDN-1
O

CH Si CH

100%

Structure of Dimethylpolysiloxane

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231

GC Columns
Polysiloxane Polymers
DB-1 Chromatograms
Aldehydes and Ketones Anabolic Steroids Anticonvulsants Aromatics Aromatics in Finished Gasoline ASTM Method 5769 EPA Air Analysis Compendium Method TO-14 Standard EPA Method 551 Esters Formaldehyde - DNPH Derivative Formaldehyde - PFBHA Derivative page 398 page 422 page 417 page 399 page 439 page 378 page 376 page 402 page 399 page 399 Fragrance Reference Standard page 381 Glycols page 404 Halogenated Hydrocarbons page 405 Nitrogen Based Solvents page 409 Polyethylene page 441 Pyrethrins page 364 Regular Unleaded Gasoline page 440 (California Phase 1) "Fast" GC Run Triethylene Glycol and Impurities page 404 Volatile Amines page 396 Volatile Sulfur Compounds page 432

DB-1
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.05 0.05 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.15 0.15 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.20 0.20 0.20 0.20 0.25

10 10 5 10 10 10 20 20 40 40 10 25 10 10 10 20 20 20 40 12 15 25 50 10

0.05 0.20 0.12 0.10 0.12 0.40 0.10 0.40 0.20 0.40 1.20 1.20 0.18 0.20 0.40 0.18 0.20 0.40 0.40 0.33 0.20 0.33 0.33 0.12

-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 280/300 -60 to 280/300 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350

126-1012 126-1013 127-100A 127-1012 127-101A 127-1013 127-1022 127-1023 127-1046 127-1043 12A-1015 12A-1025 121-1012 121-101A 121-1013 121-1022 121-102A 121-1023 121-1043 128-1012 128-10H7 128-1022 128-1052 122-10H7

GC and GC/MS

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GC Columns
Polysiloxane Polymers
DB-1 (continued)
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32

15 15 15 15 25 30 30 30 30 50 60 60 60 60 100 105 150 15 15 15 15 15 15 25 25 25 25 30 30 30 30 30 30 30 30 50 50

0.10 0.25 0.50 1.00 0.25 0.10 0.25 0.50 1.00 0.25 0.10 0.25 0.50 1.00 0.50 0.50 1.00 0.10 0.25 0.50 1.00 3.00 5.00 0.12 0.25 0.52 1.05 0.10 0.25 0.50 1.00 1.50 2.00 3.00 5.00 0.25 0.52

-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 280/300 -60 to 280/300 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 300/320 -60 to 280/300 -60 to 280/300 -60 to 280/300 -60 to 325/350 -60 to 325/350

122-1011 122-1012 122-101E 122-1013 122-1022 122-1031 122-1032 122-103E 122-1033 122-1052 122-1061 122-1062 122-106E 122-1063 122-10AE 122-10BE

GC and GC/MS

122-10G3 123-1011 123-1012 123-101E 123-1013 123-1014 123-1015 123-1027 123-1022 123-1026 123-102F 123-1031 123-1032 123-1033E 123-1033 123-103B 123-103G 123-1034 123-1035 123-1052 123-1056

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233

GC Columns
Polysiloxane Polymers
DB-1 (continued)
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.45 0.45 0.45 0.45 0.45

50 50 50 60 60 60 60 60 60 60 60 15 15 30 30 30 30 60 5 5 10 10 15 15 15 15 15 15 15 25 25 30 30 30

1.05 1.20 5.00 0.10 0.25 0.50 1.00 1.50 2.00 3.00 5.00 1.27 2.55 0.42 1.27 2.55 4.25 1.27 2.65 5.00 2.65 5.00 0.15 0.25 0.50 1.00 1.50 3.00 5.00 1.00 5.00 0.10 0.25 0.50

-60 to 325/350 -60 to 325/350 -60 to 280/300 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 300/320 -60 to 280/300 -60 to 280/300 -60 to 280/300 -60 to 300/320 -60 to 260/280 -60 to 300/320 -60 to 300/320 -60 to 260/280 -60 to 260/280 -60 to 300/320 -60 to 260/280 -60 to 260/280 -60 to 260/280 -60 to 260/280 -60 to 340/360 -60 to 320/340 -60 to 300/320 -60 to 300/320 -60 to 300/320 -60 to 260/280 -60 to 260/280 -60 to 300/320 -60 to 260/280 -60 to 340/360 -60 to 320/340 -60 to 300/320

123-105F 123-105C 123-1055 123-1061 123-1062 123-106E 123-1063 123-106B 123-106G 123-1064 123-1065 124-1012* 124-1014* 124-1037* 124-1032* 124-1034* 124-1005* 124-1062* 125-100B 125-1005 125-10HB 125-10H5 125-1011 125-101K 125-1017 125-101J 125-1012 125-1014 125-1015 125-102J 125-1025 125-1039 125-103K 125-1037

GC and GC/MS

0.45 0.45 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53

*No special ferrules required for high speed megabore 0.45 mm id columns

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GC Columns
Polysiloxane Polymers
DB-1 Order Guide (continued)
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53

30 30 30 30 30 30 50 60 60 60 60 105

0.88 1.00 1.50 2.65 3.00 5.00 5.00 1.00 1.50 3.00 5.00 5.00

-60 to 300/320 -60 to 300/320 -60 to 300/320 -60 to 260/280 -60 to 260/280 -60 to 260/280 -60 to 260/280 -60 to 300/320 -60 to 300/320 -60 to 260/280 -60 to 260/280 -60 to 260/280

125-103D 125-103J 125-1032 125-103B 125-1034 125-1035 125-1055 125-106J 125-1062 125-1064 125-1065 125-10B5

HP-1
100% Dimethylpolysiloxane Non-polar Excellent general purpose columnIndustry Standard Wide range of applications Superior performance for low molecular weight alcohols (<C5) High temperature limit Bonded and cross-linked Solvent rinsable Wide range of column dimensions available Equivalent to USP Phase G2 Similar Phases: DB-1, Ultra-1, SPB-1, CP-Sil 5 CB, Rtx-1, BP-1, OV-1, OV-101, 007-1(MS), SP-2100, SE-30, CP-Sil 5 CB MS, ZB-1, AT-1, MDN-1
CH O Si CH
3 3

GC and GC/MS

100%

Structure of Dimethylpolysiloxane

HP-1 Chromatograms
Common Industrial Solvents Denatured Fuel Ethanol-ASTM D5501 Glycols/Diols Inorganic Hydride Gases Organotin Compounds page 408 page 437 page 404 page 414 page 365 Oxygenates in Gasoline ASTM D5599 (GC-FID) Solvents Sulfur Compounds in Natural Gas Synthetic Mixture page 436 page 408 page 434

HP-1
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.20 0.20 0.20 0.20 0.20

12 25 25 25 50

0.33 0.11 0.33 0.50 0.11

-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350

19091-60312 19091Z-002 19091Z-102 19091Z-202 19091Z-005

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235

GC Columns
Polysiloxane Polymers
HP-1 (continued)
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.20 0.20 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32

50 50 15 15 15 30 30 30 60 60 100 15 15 25 25 25 30 30 30 30 30 30 50 50 50 60 60 60 5 5 5 7.5 10 10 15 15 15 15

0.33 0.50 0.10 0.25 1.00 0.10 0.25 1.00 0.25 1.00 0.50 0.25 1.00 0.17 0.52 1.05 0.10 0.25 1.00 3.00 4.00 5.00 0.17 0.52 1.05 0.25 1.00 5.00 0.15 0.88 2.65 5.00 0.88 2.65 0.15 1.50 3.00 5.00

-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 260/280 -60 to 260/280 -60 to 260/280 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 260/280 -60 to 320/400 -60 to 320/400 -60 to 260/280 -60 to 260/280 -60 to 300/320 -60 to 260/280 -60 to 320/400 -60 to 300/320 -60 to 260/280 -60 to 260/280

19091Z-105 19091Z-205 19091Z-331 19091Z-431 19091Z-231 19091Z-333 19091Z-433 19091Z-233 19091Z-436 19091Z-236 19091Z-530 19091Z-411 19091Z-211 19091Z-012 19091Z-112 19091Z-212 19091Z-313 19091Z-413 19091Z-213 19091Z-513 19091Z-613 19091Z-713 19091Z-015 19091Z-115 19091Z-215 19091Z-416 19091Z-216 19091Z-716 19095Z-220 19095Z-020 19095S-100 19095Z-627 19095Z-021 19095Z-121 19095Z-221 19095Z-321 19095Z-421 19095Z-621

GC and GC/MS

0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53

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GC Columns
Polysiloxane Polymers
HP-1 (continued)
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.53 0.53 0.53 0.53 0.53 0.53

30 30 30 30 30 60

0.88 1.50 2.65 3.00 5.00 5.00

-60 to 300/320 -60 to 300/320 -60 to 260/280 -60 to 260/280 -60 to 260/280 -60 to 260/280

19095Z-023 19095Z-323 19095Z-123 19095Z-423 19095Z-623 19095Z-626

DB-1ms
100% Dimethylpolysiloxane Identical selectivity to DB-1 Non-polar Very low bleed characteristics, ideal for GC/MS Improved acid performance compared to standard 100% Dimethylpolysiloxane columns Improved signal-to-noise ratio for better sensitivity and mass spectral integrity 340/360C upper temperature limit Excellent general purpose column Bonded and cross-linked Solvent rinsable Test mix available. See pages 9697 for test mix information. Similar Phases: HP-1ms, Rtx-1ms, CP-Sil 5 CB Low Bleed/MS, MDN-1, AT-1

CH Si CH

100%

Structure of Dimethylpolysiloxane

GC and GC/MS

DB-1ms Chromatograms
Drug Screen
page 416

DB-1ms
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.10 0.10 0.10 0.10 0.20 0.20 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32

10 10 20 20 12 25 15 30 30 60 15 30 30 60

0.10 0.40 0.10 0.40 0.33 0.33 0.25 0.10 0.25 0.25 0.25 0.10 0.25 0.25

-60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360

127-0112 127-0113 127-0122 127-0123 128-0112 128-0122 122-0112 122-0131 122-0132 122-0162 123-0112 123-0131 123-0132 123-0162

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237

GC Columns
Polysiloxane Polymers

CH Si CH

HP-1ms
100%

Structure of Dimethylpolysiloxane

100% Dimethylpolysiloxane Identical selectivity to HP-1 Non-polar Low bleed characteristics Excellent general purpose column Improved signal-to-noise ratio for better sensitivity and mass spectral integrity Bonded and cross-linked Solvent rinsable Similar Phases: HP-1ms, Rtx-1ms, CP-Sil 5 CB Low Bleed/MS, MDN-1, AT-1

HP-1ms
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.20 0.25 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.32

25 15 30 30 30 30 60 15 25 30 30 30 60

0.33 0.25 0.10 0.25 0.50 1.00 0.25 0.25 0.52 0.10 0.25 1.00 0.25

-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350

19091S-602 19091S-931 19091S-833 19091S-933 19091S-633 19091S-733 19091S-936 19091S-911 19091S-612 19091S-813 19091S-913 19091S-713 19091S-916

GC and GC/MS

DB-5
CH 3 O Si CH 3
95%

C6H5 O Si C6 H5
5%

Structure of Diphenyldimethylpolysiloxane

(5%-Phenyl)-methylpolysiloxane Non-polar Excellent general purpose column Wide range of applications Low bleed High temperature limit Bonded and cross-linked Solvent rinsable Wide range of column dimensions available Equivalent to USP Phase G27 Test mix available. See pages 9697 for test mix information. Similar Phases: HP-5, Ultra-2, SPB-5, CP-Sil 8CB, Rtx-5, BP-5, OV-5, 007-2(MPS-5), SE-52, SE-54, XTI-5, PTE-5, HP-5MS, ZB-5, AT-5, MDN-5

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GC Columns
Polysiloxane Polymers
DB-5 Chromatograms
Amphetamines and PrecursorsTMS derivatives Antihistamines Bacterial Fatty Acid Methyl Esters Cold-Pressed Orange Oil Common Drug Screen page 416 page 417 page 390 page 385 page 415 9-THC (Marijuana) and Major Metabolites-TMS Derivatives Lemon Oil Narcotics and Adulterants Over-the-Counter Pain KillersTMS Derivatives Organochlorine Pesticides page 422 page 384 page 421 page 421 page 353

DB-5
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.05 0.05 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.15 0.18 0.18 0.18 0.18 0.18 0.18 0.20 0.20 0.20 0.20 0.20 0.20 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25

10 10 5 10 10 10 10 20 20 40 10 10 10 20 20 40 40 12 15 25 25 50 50 15 15 15 15 25 25 30 30

0.05 0.20 0.12 0.10 0.17 0.33 0.40 0.10 0.40 0.20 1.20 0.18 0.40 0.18 0.40 0.18 0.40 0.33 0.20 0.11 0.33 0.11 0.33 0.10 0.25 0.50 1.00 0.25 1.20 0.10 0.25

-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 300/320 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 300/320 -60 to 325/350 -60 to 325/350

126-5012 126-5013 127-500A 127-5012 127-501E 127-501N 127-5013 127-5022 127-5023

GC and GC/MS

127-5046 12A-5015 121-5012 121-5013 121-5022 121-5023 121-5042 121-5043 128-5012 128-50H7 128-5021 128-5022 128-5051 128-5052 122-5011 122-5012 122-501E 122-5013 122-5022 122-502C 122-5031 122-5032

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239

GC Columns
Polysiloxane Polymers
DB-5 (continued)
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32

30 30 50 60 60 60 60 10 15 15 15 15 25 25 25 25 30 30 30 30 30 50 50 50 50 50 60 60 60 60 15 30 30 30 10 10 10

0.50 1.00 0.25 0.10 0.25 0.50 1.00 0.25 0.10 0.25 0.50 1.00 0.17 0.25 0.52 1.05 0.10 0.25 0.50 1.00 1.50 0.17 0.25 0.52 1.00 1.05 0.10 0.25 0.50 1.00 1.27 0.42 1.27 4.25 2.00 2.65 5.00

-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 300/320 -60 to 300/320 -60 to 300/320 -60 to 260/280 -60 to 300/320 -60 to 260/280 -60 to 260/280

122-503E 122-5033 122-5052 122-5061 122-5062 122-506E 122-5063 123-5002 123-5011 123-5012 123-501E 123-5013 123-502D 123-5022 123-5026 123-502F 123-5031 123-5032 123-503E 123-5033 123-503B 123-505D 123-5052 123-5056 123-5053 123-505f 123-5061 123-5062 123-506E 123-5063 124-5012* 124-5037* 124-5032* 124-5035* 125-50H3 125-50HB 125-50H5

GC and GC/MS

0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.45 0.45 0.45 0.45 0.53 0.53 0.53

*No special ferrules required for high speed megabore 0.45 mm id columns

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GC Columns
Polysiloxane Polymers
DB-5 (continued)
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53

15 15 15 15 15 15 25 25 30 30 30 30 30 30 30 30 50 60 60 60

0.25 0.50 1.00 1.50 3.00 5.00 2.00 5.00 0.25 0.50 0.88 1.00 1.50 2.65 3.00 5.00 1.00 1.50 3.00 5.00

-60 to 300/320 -60 to 300/320 -60 to 300/320 -60 to 300/320 -60 to 260/280 -60 to 260/280 -60 to 300/320 -60 to 260/280 -60 to 300/320 -60 to 300/320 -60 to 300/320 -60 to 300/320 -60 to 300/320 -60 to 260/280 -60 to 260/280 -60 to 260/280 -60 to 300/320 -60 to 300/320 -60 to 260/280 -60 to 260/280

125-501K 125-5017 125-501J 125-5012 125-5014 125-5015 125-5023 125-5025 125-503K 125-5037 125-503D 125-503J 125-5032 125-503B 125-5034 125-5035

GC and GC/MS

125-505J 125-5062 125-5064 125-5065

HP-5
(5%-Phenyl)-methylpolysiloxane Non-polar Excellent general purpose column Wide range of applications High temperature limit Bonded and cross-linked Solvent rinsable Wide range of column dimensions available Equivalent to USP Phase G27 Similar Phases: DB-5, Ultra-2, SPB-5, CP-Sil 8 CB, Rtx-5, BP-5, OV-5, 007-2(MPS-5), SE-52, SE-54, XTI-5, PTE-5, HP-5MS, ZB-5, AT-5, MDN-5
CH 3 O Si CH 3
95%

C6H5 O Si C6 H5
5%

Structure of Diphenyldimethylpolysiloxane

HP-5 Chromatograms
Organomercury Compounds Organotin Compounds
page 365 page 365

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241

GC Columns
Polysiloxane Polymers
HP-5
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.32 0.32

12 25 25 25 50 50 50 15 15 30 30 30 60 60 15 15 25 25 25 30 30 30 30 30 50 50 50 60 60 10 15 15 30 30 30 30

0.33 0.11 0.33 0.50 0.11 0.33 0.50 0.25 1.00 0.10 0.25 1.00 0.25 1.00 0.25 1.00 0.17 0.52 1.05 0.10 0.25 0.50 1.00 1.00 0.17 0.52 1.05 0.25 1.00 2.65 1.50 5.00 0.88 1.50 2.65 5.00

-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 260/280 60 to 300/320 -60 to 260/280 60 to 300/320 60 to 300/320 -60 to 260/280 -60 to 260/280

19091J-101 19091J-002 19091J-102 19091J-202 19091J-005 19091J-105 19091J-205 19091J-431 19091J-231 19091J-333 19091J-433 19091J-233 19091J-436 19091J-236 19091J-411 19091J-211 19091J-012 19091J-112 19091J-212 19091J-313 19091J-413 19091J-113 19091J-213 19091J-213E 19091J-015 19091J-115E 19091J-215 19091J-416 19091J-216 19095J-121 19095J-321 19095J-621 19095J-023 19095J-323 19095J-123 19095J-623

GC and GC/MS

0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.53 0.53 0.53 0.53 0.53 0.53 0.53

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GC Columns
Polysiloxane Polymers

DB-5ms
Phenyl Arylene polymer virtually equivalent to a (5%-Phenyl)-methylpolysiloxane Non-polar Very low bleed characteristics, ideal for GC/MS Excellent inertness for active compounds Improved signal-to-noise ratio for better sensitivity and mass spectral integrity Bonded and cross-linked Solvent rinsable Ion trap testing and certification available Exact replacement of HP-5TA Close equivalent to USP Phase G27 Test mix available. See pages 9697 for test mix information. Similar Phases: Rtx-5MS, HP-5MS, PTE-5, CP-Sil 8 CB low bleed/MS, BPX-5, AT-5MS
CH 3 O Si CH 3 CH 3 Si CH 3
m

CH 3 O Si CH 3
n

Structure of poly(dimethylsiloxy)poly(1,4-bis (dimethylsiloxy) phenylene)siloxane

DB-5ms Chromatograms
50 ppb Formaldehyde page 380 Amines and Nitriles page 397 Diesel Fuel page 348 EPA Air Analysis Compendium page 379 Method TO-15 (1 ppV Standard) EPA Method 525.2 page 367 EPA Method 551.1 page 377 EPA Method 8061 page 368 (Phthalate Esters) Narcotics page 420 Organochlorine Pesticides (EPA Method 8081A) Organophosphorous Pesticides (EPA Method 8141 A) Phenols Phenols Polyethyleneamines Substituted Anilines Sulfur in Air page 350 page 356 page 369 page 412 page 396 page 411 page 380

GC and GC/MS

DB-5ms
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.18 0.20 0.20 0.20 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25

40 12 25 50 15 15 15 15 25 25 30 30 30 30

0.18 0.33 0.33 0.33 0.10 0.25 0.50 1.00 0.25 0.40 0.10 0.25 0.50 1.00

-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350

121-5542 128-5512 128-5522 128-5552 122-5511 122-5512 122-5516 122-5513 122-5522 122-552A 122-5531 122-5532 122-5536 122-5533

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243

GC Columns
Polysiloxane Polymers
DB-5ms (continued)
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32

50 60 60 15 15 15 25 30 30 30 30 50 50 60 60 60 60 15 15 15 30 30 30

0.25 0.10 0.25 0.10 0.25 1.00 0.52 0.10 0.25 0.50 1.00 0.25 1.00 0.10 0.25 0.50 1.00 0.50 1.00 1.50 0.50 1.00 1.50

-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 300/320 -60 to 300/320 -60 to 300/320 -60 to 300/320 -60 to 300/320 -60 to 300/320

122-5552 122-5561 122-5562 123-5511 123-5512 123-5513 123-5526 123-5531 123-5532 123-5536 123-5533 123-5552 123-5553 123-5561 123-5562 123-5566 123-5563 125-5517 125-551J 125-5512 125-5537 125-553J 125-5532

GC and GC/MS

0.32 0.53 0.53 0.53 0.53 0.53 0.53

DB-5ms.ITD
0.25 30 0.25 -60 to 325/350 122-4132

HP-5ms
CH 3 O Si CH 3
95%

C6H5 O Si C6H5
5%

Structure of Diphenyldimethylpolysiloxane

(5%-Phenyl)-methylpolysiloxane Identical selectivity to HP-5 Non-polar Very low bleed characteristics, ideal for GC/MS Excellent inertness for active compounds including acidic and basic compounds Improved signal-to-noise ratio for better sensitivity and mass spectral integrity Bonded and cross-linked Solvent rinsable Equivalent to USP Phase G27 Similar Phases: Rtx-5MS, Rtx-5 Amine, DB-5MS, PTE-5, CP-Sil 8 CB low bleed/MS, BPX-5, AT-5, MDN-5

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GC Columns
Polysiloxane Polymers
HP-5ms Chromatograms
Chlorinated Pesticides: page 352 EPA Method 508 Nitrogen/Phosphorus Containing page 356 Pesticides: EPA Method 507 Organohalide Pesticides in Water, EPA Method 505 Phenols on HP-5MS USEPA Method 8270 Semivolatile Compounds page 352 page 412 page 366

HP-5ms
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.20 0.20 0.20 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32

12 25 50 15 15 15 30 30 30 30 60 60 15 15 15 25 30 30 30 30 60 60

0.33 0.33 0.33 0.10 0.25 1.00 0.10 0.25 0.50 1.00 0.10 0.25 0.10 0.25 1.00 0.52 0.10 0.25 0.50 1.00 0.10 0.25

-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350

19091S-101 19091S-102 19091S-105 19091S-331 19091S-431 19091S-231 19091S-333 19091S-433 19091S-133 19091S-233

GC and GC/MS

19091S-336 19091S-436 19091S-311 19091S-411 19091S-211 19091S-112 19091S-313 19091S-413 19091S-113 19091S-213 19091S-316 19091S-416

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245

GC Columns
Polysiloxane Polymers

DB-XLB
EXceptionally Low Bleed Low polarity Extended temperature limit of 340/360C Unique selectivity Excellent inertness for active compounds Ideal for confirmational analyses Excellent for pesticides, herbicides, PCBs and PAHs Ideal for GC/MS Ion trap testing and certification available Bonded and cross-linked Solvent rinsable Test mix available. See pages 9697 for test mix information. Similar Phase: MDN-12 Note: DB-XLB is designed for inhibiting column bleed at high temperatures. It also appears to have inadvertently inherited an exceptional ability for separating many PCB congeners when used with MS detection. This stellar performance was maximized after careful optimization of the column dimensions, temperature programs, and carrier gas flow conditions... (Frame, G. Analytical Chemistry News & Features, Aug. 1, 1997, 468A-475A)

GC and GC/MS

DB-XLB Chromatograms
Aroclors 1016-1268 (without 1221) CLP Pesticides PCB Congeners in DIN Method EPA 508.1 Pesticides EPA 552.2 EPA 8151A Phenoxy Acid Herbicides - Methyl Derivatives page 361 page 349 page 362 page 351 page 370 page 357 Garlic Oil Herbicides PCBs by EPA Method 8082 Phenols Ylang Ylang Oil page 384 page 358 page 363 page 369 page 386

246

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GC Columns
Polysiloxane Polymers
DB-XLB
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.18 0.18 0.18 0.20 0.20 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.45 0.53 0.53

20 30 60 12 25 15 15 15 30 30 30 30 60 15 15 30 30 30 30 60 30 15 30

0.18 0.18 0.18 0.33 0.33 0.10 0.25 1.00 0.10 0.25 0.50 1.00 0.25 0.25 1.00 0.10 0.25 0.50 1.00 0.25 1.27 1.50 1.50

30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 320/340 30 to 320/340 30 to 320/340

121-1222 121-1232 121-1262 128-1212 128-1222 122-1211 122-1212 122-1213 122-1231 122-1232 122-1236 122-1233 122-1262 123-1212 123-1213 123-1231

GC and GC/MS

123-1232 123-1236 123-1233 123-1262 124-1232* 125-1212 125-1232

*No special ferrules required for high speed megabore 0.45 mm id columns

DB-XLB.ITD
0.25 30 0.25 30 to 340/360 122-4232

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247

GC Columns
Polysiloxane Polymers

DB-35
(35%-Phenyl)-methylpolysiloxane Midpolarity slightly more polar than HP-35 Low bleed Inert to active solutes Ideal for confirmational analyses Bonded and cross-linked Solvent rinsable Equivalent to USP Phase G42 Test mix available. See pages 9697 for test mix information. Similar Phases: Rtx-35, SPB-35, AT-35, Sup-Herb, HP-35, BPX-35

DB-35
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.53 0.53 0.53 0.53 0.53

30 30 30 60 30 30 30 60 15 30 30 60 60

0.25 0.50 1.00 0.25 0.25 0.50 1.00 0.25 1.00 0.50 1.00 1.00 1.50

40 to 300/320 40 to 300/320 40 to 280/300 40 to 300/320 40 to 300/320 40 to 300/320 40 to 300/320 40 to 300/320 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300

122-1932 122-1933 122-1934 122-1962 123-1932 123-1933 123-1934 123-1962 125-1912 125-1937 125-1932 125-1962 125-1963

GC and GC/MS

248

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GC Columns
Polysiloxane Polymers

HP-35
(35%-Phenyl)-methylpolysiloxane Midpolarity slightly less polar than DB-35 Inert to active solutes Ideal for confirmational analyses Bonded and cross-linked Solvent rinsable Equivalent to USP Phase G42 Similar Phases: Rtx-35, SPB-35, AT-35, Sup-Herb, DB-35, BPX-35

HP-35 Chromatograms
Primary Amines page 396

HP-35
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.25 0.25 0.25 0.32 0.32 0.32

15 30 60 30 30 30

0.25 0.25 0.25 0.15 0.25 0.50

40 to 300/320 40 to 300/320 40 to 300/320 40 to 300/320 40 to 300/320 40 to 300/320

19091G-131 19091G-133 19091G-136 19091G-013 19091G-113

GC and GC/MS

19091G-213

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249

GC Columns
Polysiloxane Polymers

DB-35ms
Equivalent to a (35%-Phenyl)-methylpolysiloxane Midpolarity Very low bleed characteristics, ideal for GC/MS Extended temperature limit of 340/360C Excellent inertness for active compounds Ideal for confirmational analyses Bonded and cross-linked Solvent rinsable Replaces HP-35ms Close equivalent to USP Phase G42 Test mix available. See pages 9697 for test mix information. Similar Phases: Rtx-35, SPB-35, AT-35, Sup-Herb, MDN-35, BPX-35

DB-35ms Chromatograms
Anilines Barbiturates Benzodiazepines Chlordane CLP Pesticides EPA 508.1 Pesticides EPA 552.2 EPA 8151A Phenoxy Acid Herbicides - Methyl Derivatives page 411 page 418 page 419 page 364 page 349 page 351 page 370 page 357 Organochlorine Pesticides (EPA Method 8081A) Organophosphorous Pesticides (EPA Method 8141 A) PCBs by EPA Method 8082 Toxaphene page 350 page 357 page 363 page 364

GC and GC/MS

DB-35ms
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.20 0.20 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.53 0.53 0.53

15 25 15 30 30 60 15 30 60 15 30 30

0.33 0.33 0.25 0.15 0.25 0.25 0.25 0.25 0.25 1.00 0.50 1.00

50 to 340/360 50 to 340/360 50 to 340/360 50 to 340/360 50 to 340/360 50 to 340/360 50 to 340/360 50 to 340/360 50 to 340/360 50 to 320/340 50 to 320/340 50 to 320/340

128-3812 128-3822 122-3812 122-3831 122-3832 122-3862 123-3812 123-3832 123-3862 125-3812 125-3837 125-3832

250

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GC Columns
Polysiloxane Polymers

DB-17
(50%-Phenyl)-methylpolysiloxane Midpolarity slightly more polar than HP-50+ Excellent for confirmational analyses Bonded and cross-linked Solvent rinsable Equivalent to USP Phase G3 Test mix available. See pages 9697 for test mix information. Similar Phases: HP-50+, Rtx-50, CP-Sil 24 CB, 007-17(MPS-50), HP-17, SP-2250, SPB-50, ZB-50, AT-50
CH 3 O Si CH 3
50%

C6H5 O Si C6H5
50%

Structure of Diphenyldimethylpolysiloxane

DB-17 Chromatograms
Common Drug Screen Free Steroids page 415 page 421 Triazine Herbicides page 359

DB-17
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.05 0.10 0.10 0.10 0.10 0.18 0.18 0.18 0.18 0.20 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32

10 10 10 20 20 10 10 20 20 25 15 15 15 30 30 30 60 60 15 15 15 30

0.10 0.10 0.20 0.10 0.20 0.18 0.30 0.18 0.30 0.16 0.15 0.25 0.50 0.15 0.25 0.50 0.25 0.50 0.15 0.25 0.50 0.15

40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300

126-1713 127-1712 127-1713

GC and GC/MS

127-1722 127-1723 121-1712 121-1713 121-1722 121-1723 128-1721 122-1711 122-1712 122-1713 122-1731 122-1732 122-1733 122-1762 122-1763 123-1711 123-1712 123-1713 123-1731

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251

GC Columns
Polysiloxane Polymers
DB-17 (continued)
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.32 0.32 0.32 0.32 0.45 0.45 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53

30 30 60 60 15 30 5 15 15 15 15 15 30 30 30 30 60 60

0.25 0.50 0.25 0.50 0.85 0.85 2.00 0.10 0.25 0.50 1.00 1.50 0.25 0.50 1.00 1.50 1.00 1.50

40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 260/280 40 to 260/280 40 to 260/280 40 to 260/280 40 to 260/280 40 to 280/300 40 to 260/280 40 to 260/280 40 to 260/280 40 to 260/280 40 to 260/280 40 to 260/280 40 to 260/280 40 to 260/280

123-1732 123-1733 123-1762 123-1763 124-1712* 124-1732* 125-1704 125-1719 125-1711 125-1717 125-1712 125-1713 125-1731 125-1737 125-1732 125-1733 125-1762 125-1763

GC and GC/MS

0.53 0.53

*No special ferrules required for high speed megabore 0.45 mm id columns

HP-50 +
(50%-Phenyl)-methylpolysiloxane Midpolarity-slightly less polar than DB-17 Excellent for confirmational analyses Bonded and cross-linked Solvent rinsable Equivalent to USP Phase G3 Similar Phases: DB-17, Rtx-50, CP-Sil 24 CB, 007-17(MPS-50), SP-2250, SPB-50, ZB-50, AT-50

HP-50+
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.20 0.20 0.20 0.20 0.20 0.25 0.25 0.25 0.25 0.25

12 12 25 25 50 15 15 15 30 30

0.16 0.31 0.16 0.31 0.31 0.15 0.25 0.50 0.15 0.25

40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300

19091L-001 19091L-101 19091L-002 19091L-102 19091L-105 19091L-331 19091L-431 19091L-131 19091L-333 19091L-433

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GC Columns
Polysiloxane Polymers
HP-50+ (continued)
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.53 0.53 0.53

30 60 15 15 30 30 30 60 60 15 30 30

0.50 0.25 0.25 0.50 0.15 0.25 0.50 0.25 0.50 1.00 0.50 1.00

40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 260/280 40 to 260/280 40 to 260/280

19091L-133 19091L-436 19091L-411 19091L-111 19091L-313 19091L-413 19091L-113 19091L-416 19091L-116 19095L-021 19095L-523 19095L-023

DB-17ms
Virtually equivalent to (50%-Phenyl)-methylpolysiloxane 320/340C upper temperature limit Very low bleed midpolarity column, ideal for GC/MS Excellent inertness for active compunds Enhanced mass spectral integrity Bonded and cross-linked Solvent rinsable Best column for CLP pesticides Test mix available. See pages 9697 for test mix information. Similar Phases: HP-50+, Rtx-50, 007-17, SP-2250, SPB-50, BPX-50, SPB-17, AT-50

GC and GC/MS

DB-17ms Chromatograms
Hallucinogens PAHs page 420 page 368 Tocopherols page 419

DB-17ms
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.18 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32

20 15 15 30 30 60 15 15 30 30

0.18 0.15 0.25 0.15 0.25 0.25 0.15 0.25 0.15 0.25

40 to 320/340 40 to 320/340 40 to 320/340 40 to 320/340 40 to 320/340 40 to 320/340 40 to 320/340 40 to 320/340 40 to 320/340 40 to 320/340

121-4722 122-4711 122-4712 122-4731 122-4732 122-4762 123-4711 123-4712 123-4731 123-4732

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253

GC Columns
Polysiloxane Polymers

DB-23
CN CH 2 CH 2 CH 2 O Si CH 3 n

(50%-Cyanopropyl)-methylpolysiloxane High polarity Designed for separation of fatty acid methyl esters (FAMEs) Excellent resolution for cis- and trans-isomers Bonded and cross-linked Solvent rinsable Replaces HP-23 Close equivalent to USP Phase G5 Test mix available. See pages 9697 for test mix information. Similar Phases: SP-2330, Rtx-2330, 007-23, AT-Silar, CP-Sil 88, BPX-70, SP-2340

Structure of Cyanopropylmethylpolysiloxane

DB-23 Chromatograms
Canola Oil Margarine Partially Hydrogenated FAMEs page 393 FAMEs page 391

DB-23
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.18 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.53 0.53

20 15 30 30 60 60 15 30 30 60 60 15 30

0.20 0.25 0.15 0.25 0.15 0.25 0.25 0.15 0.25 0.15 0.25 0.50 0.50

40 to 250/260 40 to 250/260 40 to 250/260 40 to 250/260 40 to 250/260 40 to 250/260 40 to 250/260 40 to 250/260 40 to 250/260 40 to 250/260 40 to 250/260 40 to 230/240 40 to 230/240

121-2323 122-2312 122-2331 122-2332 122-2361 122-2362 123-2312 123-2331 123-2332 123-2361 123-2362 125-2312 125-2332

GC and GC/MS

DB-200
CF 3 CH 2 CH 2 O Si CH 3 n

(35% Trifluoropropyl)-methylpolysiloxane 300/320C temperature limit Midpolarity (more polar than DB-1701 or DB-17) Ideal for difficult to separate positional isomers Unique interactions with compounds containing nitro, halogen and carbonyl groups Low ECD bleed Unique selectivity Close equivalent to USP Phase G6 Test mix available. See pages 9697 for test mix information. Similar Phases: Rtx-200

Structure of Trifluoropropylmethylpolysiloxane

254

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GC Columns
Polysiloxane Polymers
DB-200 Chromatograms
Acrylate Impurities Aromatic Solvents page 410 page 407 Solvents page 408

DB-200
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.18 0.25 0.25 0.25 0.25 0.32 0.32 0.45 0.53 0.53 0.53

20 15 15 30 30 30 30 30 15 30 30

0.40 0.25 0.50 0.25 0.50 0.25 0.50 0.85 1.00 0.50 1.00

30 to 300/320 30 to 300/320 30 to 300/320 30 to 300/320 30 to 300/320 30 to 300/320 30 to 300/320 30 to 280/300 30 to 280/300 30 to 280/300 30 to 280/300

121-2023 122-2012 122-2013 122-2032 122-2033 123-2032 123-2033 124-2032* 125-2012 125-2037 125-2032

*No special ferrules required for high speed megabore 0.45 mm id columns

DB-210
(50%-Trifluoropropyl)-methylpolysiloxane High polarity Excellent for U.S. EPA Methods 8140 and 609 Bonded and cross-linked Solvent rinsable Exact replacement of HP-210 Close equivalent to USP Phase G6 Test mix available. See pages 9697 for test mix information. Similar Phases: SP-2401
CF3 CH 2 CH 2 O Si CH 3 n

GC and GC/MS

Structure of Trifluoropropylmethylpolysiloxane

DB-210
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.18 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.53 0.53

20 15 30 30 15 15 30 30 30 15 30

0.30 0.25 0.25 0.50 0.25 0.50 0.15 0.25 0.50 1.00 1.00

45 to 240/260 45 to 240/260 45 to 240/260 45 to 240/260 45 to 240/260 45 to 240/260 45 to 240/260 45 to 240/260 45 to 240/260 45 to 220/240 45 to 220/240

121-0223 122-0212 122-0232 122-0233 123-0212 123-0213 123-0231 123-0232 123-0233 125-0212 125-0232

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255

GC Columns
Polysiloxane Polymers

DB-225
CN CH 2 CH 2 CH 3 O Si CH 3
50%

CH 2 O Si C6H5
50%

(50%-Cyanopropylphenyl)-dimethylpolysiloxane Mid/high polarity Excellent for separations of cis- and trans-fatty acid methyl esters (FAMEs) Bonded and cross-linked Solvent rinsable Exact replacement of HP-225 Close equivalent to USP Phase G7 Test mix available. See pages 9697 for test mix information. Similar Phases: SP-2330, CP-Sil 43 CB, Rtx-225, BP-225, OV-225, 007-225, AT-225

Structure of Cyanopropylphenylmethylpolysiloxane

DB-225 Chromatograms
Alditol Acetates FAME Standard page 388 page 392 Tetrachlorodibenzo-p-furans page 359

DB-225
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.10 0.18 0.25

20 20 15 30 30 15 30 30 15 30 30

0.10 0.20 0.25 0.15 0.25 0.25 0.15 0.25 1.00 0.50 1.00

40 to 220/240 40 to 220/240 40 to 220/240 40 to 220/240 40 to 220/240 40 to 220/240 40 to 220/240 40 to 220/240 40 to 200/220 40 to 200/220 40 to 200/220

127-2222 121-2223 122-2212 122-2231 122-2232 123-2212 123-2231 123-2232 125-2212 125-2237 125-2232

GC and GC/MS

0.25 0.25 0.32 0.32 0.32 0.53 0.53 0.53

DB-225ms
Virtually equivalent to (50%-Cyanopropylphenyl)-methylpolysiloxane Mid/high polarity Excellent for separations of cis- and trans-fatty acid methyl esters (FAMEs) Low bleed Bonded and cross-linked Solvent rinsable Close equivalent to USP Phase G7 Similar Phases: HP-225, SP-2330, CP-Sil 43 CB, Rtx-225, BP-225, OV-225, 007-225, AT-225

DB-225ms Chromatograms
Tetrachlorodibenzo-p-furans page 359

256

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GC Columns
Polysiloxane Polymers
DB-225ms
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.25 0.25 0.25 0.32 0.32

15 30 60 15 30

0.25 0.25 0.25 0.25 0.25

40 to 240 40 to 240 40 to 240 40 to 240 40 to 240

122-2912 122-2932 122-2962 123-2912 123-2932

DB-624
Specifically designed for the analysis of volatile priority pollutants No cryogenics needed for U.S. EPA Method 502.2 Excellent for U.S. EPA Methods: 501.3, 502.2, 503.1, 524.2, 601, 602, 8010, 8015, 8020, 8240, 8260 Excellent inertness for active compounds Bonded and cross-linked Solvent rinsable Exact replacement of HP-624 Equivalent to USP Phase G43 Test mix available. See pages 9697 for test mix information. Similar Phases: AT-624, Rtx-624, PE-624, 007-624, 007-502, CP-624, ZB-624

GC and GC/MS

DB-624 Chromatograms
1,3-Butadiene Alcohols EPA Method 504 by GC/MS EPA Volatiles GC/MS EPA Method 8021 Esters page 428 page 394 page 376 page 371 page 373 page 402 Ethers Fast VOA Analysis Glycols Halogenated Hydrocarbon Nitrogen Based Solvents Residual Solvents, USP <467> page 403 page 375 page 403 page 405 page 409 page 424

DB-624
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.18 0.20 0.25 0.25 0.32 0.32 0.45 0.45 0.53 0.53 0.53

20 25 30 60 30 60 30 75 30 60 75

1.00 1.12 1.40 1.40 1.80 1.80 2.55 2.55 3.00 3.00 3.00

-20 to 260 -20 to 260 -20 to 260 -20 to 260 -20 to 260 -20 to 260 -20 to 260 -20 to 260 -20 to 260 -20 to 260 -20 to 260

121-1324 128-1324 122-1334 122-1364 123-1334 123-1364 124-1334* 124-1374* 125-1334 125-1364 125-1374

*No special ferrules required for high speed megabore 0.45 mm id columns

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257

GC Columns
Polysiloxane Polymers

DB-1301 and DB-1701


DB-1301: (6%-Cyanopropyl-phenyl) methylpolysiloxane DB-1301: Equivalent to USP Phase G43 DB-1701: (14%-Cyanopropyl-phenyl)-methylpolysiloxane Low/midpolarity Bonded and cross-linked Exact replacement of HP-1301 and HP-1701 Solvent rinsable Test mix available. See pages 9697 for test mix information. Similar Phases to DB-1301: Rtx-1301, PE-1301 Similar Phases to DB-1701: SPB-1701, CP-Sil 19 CB, Rtx-1701, BP-10, OV-1701, 007-1701, ZB-1701

DB-1301
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.18 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.32 0.53 0.53 0.53 0.53 0.53

10 15 15 30 30 30 60 60 15 15 30 30 60 60 15 15 30 30 60

0.40 0.25 1.00 0.25 0.50 1.00 0.25 1.00 0.25 1.00 0.25 1.00 0.25 1.00 1.00 1.50 1.00 1.50 1.50

-20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 260/280 -20 to 260/280 -20 to 260/280 -20 to 260/280 -20 to 260/280

121-1313 122-1312 122-1313 122-1332 122-1336 122-1333 122-1362 122-1363 123-1312 123-1313 123-1332 123-1333 123-1362 123-1363 125-1312 125-1313 125-1332 125-1333 125-1363

GC and GC/MS

DB-1701 Chromatograms
Acrylate Impurities Fentanyls Organochlorine Pesticides page 410 page 419 page 354 Phenoxy Acid Herbicides TMS Sugars Triazine Herbicides page 358 page 389 page 359

258

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GC Columns
Polysiloxane Polymers
DB-1701
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.10 0.10 0.18 0.18 0.20 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.45 0.45 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53

20 20 10 20 25 15 15 15 30 30 30 60 60 60 60 15 15 15 30 30 30 30 50 60 60 60 30 30 15 15 15 30 30 30 30 30 60 60

0.10 0.40 0.40 0.40 0.20 0.15 0.25 1.00 0.15 0.25 1.00 0.15 0.25 0.50 1.00 0.15 0.25 1.00 0.10 0.15 0.25 1.00 1.00 0.15 0.25 1.00 0.42 0.85 0.25 0.50 1.00 0.10 0.25 0.50 1.00 1.50 1.00 1.50

-20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 260/280 -20 to 260/280 -20 to 260/280 -20 to 260/280 -20 to 260/280 -20 to 260/280 -20 to 260/280 -20 to 260/280 -20 to 260/280 -20 to 260/280 -20 to 260/280 -20 to 260/280

127-0722 127-0723 121-0713 121-0723 128-0722 122-0711 122-0712 122-0713 122-0731 122-0732 122-0733 122-0761 122-0762 122-0766 122-0763 123-0711

GC and GC/MS

123-0712 123-0713 123-0739 123-0731 123-0732 123-0733 123-0753 123-0761 123-0762 123-0763 124-0737* 124-0732* 125-0711 125-0717 125-0712 125-0739 125-0731 125-0737 125-0732 125-0733 125-0762 125-0763

*No special ferrules required for high speed megabore 0.45 mm id columns

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259

GC Columns
PEG Columns

H -HO C H

H C H O n H

Polyethylene Glycol (PEG) Columns


Agilent offers a full range of PEG columns. Even though each phase is based on the polyethylene glycol polymer, strict control of the cross-linking and deactivation processes results in a variety of unique phase characteristics to meet the varying analysis needs of your laboratory.
PEG Column Features Lowest operating temperature limit Most similar to Carbowax 20M Available in 0.10 mm id Highly inert Benefits Analyze low boiling point analytes Transfer older methods to bonded phase Used for Fast GC for high sample throughput Broad analyte compatibility Compromise for high and low boiling analytes Analyze high boiling compounds Excellent general purpose column Best choice for MS use Broad analyte compatibility Can inject organic acids without derivitization Good peak shape for basic compounds *Cannot be solvent rinsed

Structure of Polyethylene Glycol

DB-WAX

DB-WAXetr HP-INNOWax

Middle operating temperature range Highest upper temperature limit Wide chemical compatibility Lowest bleed at elevated temperatures Highly inert Acid modified

DB-FFAP, HP-FFAP

GC and GC/MS

CAM (see page 296) Base modified *Non-bonded

DB-WAX
Polyethylene glycol (PEG) Close equivalent to USP Phase G16 High polarity Lower temperature limit of 20C is lowest of any bonded PEG phase; improves resolution of low boiling point analytes Column-to-column reproducibility Bonded and cross-linked Exact replacement of HP-WAX Solvent rinsable Test mix available. See pages 9697 for test mix information. Similar Phases: HP-20M, SUPELCOWAX 10, CP-WAX 52 CB, SUPEROX II, CB-WAX, Stabilwax, BP-20, 007-CW, Carbowax, HP-INNOWax, Rtx-WAX, ZB-WAX

260

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GC Columns
PEG Columns
DB-WAX Chromatograms
Aldehydes and Ketones Aromatics Ethylene Oxide FAME Standard Fast Styrene Analysis Formaldehyde-Underivatized page 398 page 400 page 414 page 392 page 436 page 399 Fragrance Reference Standard Glycols Peppermint Oil Phenols Spearmint Oil (Western) page 382 page 403 page 385 page 413 page 386

DB-WAX
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.05 0.05 0.10 0.10 0.10 0.10 0.18 0.18 0.18 0.18 0.18 0.20 0.20 0.20 0.20 0.20 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25

10 10 10 10 20 20 10 10 20 20 40 25 25 50 50 60 15 15 15 30 30 30 60 60 60

0.05 0.10 0.10 0.20 0.10 0.20 0.18 0.30 0.18 0.30 0.30 0.20 0.20 0.20 0.40 0.20 0.15 0.25 0.50 0.15 0.25 0.50 0.15 0.25 0.50

20 to 250/260 20 to 240/250 20 to 250/260 20 to 240/250 20 to 250/260 20 to 240/250 20 to 250/260 20 to 240/250 20 to 250/260 20 to 240/250 20 to 240/250 20 to 250/260 20 to 250/260 20 to 250/260 20 to 240/250 20 to 250/260 20 to 250/260 20 to 250/260 20 to 240/250 20 to 250/260 20 to 250/260 20 to 240/250 20 to 250/260 20 to 250/260 20 to 240/250

126-7012 126-7013 127-7012 127-7013 127-7022 127-7023 121-7012 121-7013 121-7022 121-7023 121-7043 128-7022 128-7032 128-7052 128-7053 128-7062 122-7011 122-7012 122-7013 122-7031 122-7032 122-7033 122-7061 122-7062 122-7063

GC and GC/MS

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261

GC Columns
PEG Columns
DB-WAX (continued)
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.45 0.45 0.45 0.53 0.53 0.53 0.53 0.53

15 15 15 30 30 30 60 60 15 30 60 15 15 30 30 30 60

0.15 0.25 0.50 0.15 0.25 0.50 0.25 0.50 0.85 0.85 0.85 0.50 1.00 0.25 0.50 1.00 1.00

20 to 250/260 20 to 250/260 20 to 240/250 20 to 250/260 20 to 250/260 20 to 240/250 20 to 250/260 20 to 240/250 20 to 230/240 20 to 230/240 20 to 230/240 20 to 230/240 20 to 230/240 20 to 230/240 20 to 230/240 20 to 230/240 20 to 230/240

123-7011 123-7012 123-7013 123-7031 123-7032 123-7033 123-7062 123-7063 124-7012* 124-7032* 124-7062* 125-7017 125-7012 125-7031 125-7037 125-7032 125-7062

GC and GC/MS

0.53

*No special ferrules required for high speed megabore 0.45 mm id columns

DB-WAXetr
Polyethylene glycol (PEG) Extended Temperature Range (etr) High polarity Excellent column-to-column repeatability Bonded and cross-linked Solvent rinsable Close equivalent to USP Phase G16 Test mix available. See pages 9697 for test mix information. Similar Phases: HP-20M, SUPELCOWAX 10, CP-WAX 52 CB, SUPEROX II, CB-WAX, Stabilwax, BP-20, 007-CW, Carbowax, HP-INNOWax, ZB-WAX

262

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GC Columns
PEG Columns
DB-WAXetr Chromatograms
Alcohols Impurities in Mixed Xylenes Impurities in Styrene page 394 page 401 page 401 Organic Acids Solvents Solvents page 395 page 406 page 407

DB-WAXetr
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.20 0.25 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.45 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53

25 15 15 30 30 60 60 15 15 15 30 30 30 50 60 60 60 5 5 15 15 30 30 30 60 60

0.40 0.25 0.50 0.25 0.50 0.25 0.50 0.25 0.50 1.00 0.25 0.50 1.00 1.00 0.25 0.50 1.00 1.70 2.00 1.00 2.00 1.00 1.50 2.00 1.00 1.50

30 to 250/260 30 to 260/280 30 to 250/260 30 to 260/280 30 to 250/260 30 to 260/280 30 to 250/260 30 to 260/280 30 to 250/260 30 to 250/260 30 to 260/280 30 to 250/260 30 to 250/260 30 to 250/260 30 to 260/280 30 to 250/260 30 to 250/260 50 to 230/250 50 to 230/250 30 to 240/260 50 to 230/250 30 to 240/260 30 to 230/240 50 to 230/250 30 to 240/260 30 to 230/240

128-7323 122-7312 122-7313 122-7332 122-7333 122-7362 122-7363 123-7312 123-7313 123-7314 123-7332 123-7333

GC and GC/MS

123-7334 123-7354 123-7362 123-7363 123-7364 124-7304* 125-7304 125-7312 125-7314 125-7332 125-7333 125-7334 125-7362 125-7363

*No special ferrules required for high speed megabore 0.45 mm id columns

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263

GC Columns
PEG Columns

HP-INNOWax
Polyethylene glycol (PEG) High polarity Highest upper temperature limits of the bonded PEG phases Column-to-column repeatability Bonded and cross-linked Solvent rinsable Close equivalent to USP Phase G16 Similar Phases: HP-20M, SUPELCOWAX 10, CP-WAX 52 CB, SUPEROX II, CB-WAX, Stabilwax, BP-20, 007-CW, Carbowax, DB-WAXetr, ZB-WAX, DB-WAXetr

HP-INNOWax Chromatograms
Alcohols Aldehydes and Acids Aromatics Anlalysis-Ethylbenzene Impurities ASTM D16 Aromatics Analysis Bourbon Chlorinated Isooctane Esters Free Fatty Acids Free Organic Acids/C4-C5 Isomers page 395 page 397 page 435 page 435 page 388 page 406 page 402 page 389 page 395 Impurities in Ethylbenzene Impurities in p-Xylene-ASTM D3798 Monomers in Latex Paint by Headspace Perfume Polyunsaturated FAMEs Rapid Analysis of Water in Organic Solvents Strawberry Syrup Sulfur and Selenium in Garlic by Headspace page 401 page 435 page 410 page 383 page 391 page 409 page 388 page 389

GC and GC/MS

HP-INNOWax
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.20 0.20 0.20 0.20 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.32

25 25 50 50 15 15 30 30 30 60 60 60 15 15 30 30 30 60

0.20 0.40 0.20 0.40 0.25 0.50 0.15 0.25 0.50 0.15 0.25 0.50 0.25 0.50 0.15 0.25 0.50 0.15

40 to 260/270 40 to 260/270 40 to 260/270 40 to 260/270 40 to 260/270 40 to 260/270 40 to 260/270 40 to 260/270 40 to 260/270 40 to 260/270 40 to 260/270 40 to 260/270 40 to 260/270 40 to 260/270 40 to 260/270 40 to 260/270 40 to 260/270 40 to 260/270

19091N-102 19091N-202 19091N-105 19091N-205 19091N-131 19091N-231 19091N-033 19091N-133 19091N-233 19091N-036 19091N-136 19091N-236 19091N-111 19091N-211 19091N-013 19091N-113 19091N-213 19091N-016

264

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GC Columns
PEG Columns
HP-INNOWax (continued)
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.32 0.32 0.53 0.53 0.53

60 60 15 30 60

0.25 0.50 1.00 1.00 1.00

40 to 260/270 40 to 260/270 40 to 240/250 40 to 240/250 40 to 240/250

19091N-116 19091N-216 19095N-121 19095N-123 19095N-126

DB-FFAP
Nitroterephthalic acid modified polyethylene glycol High polarity Temperature range from 40 to 250C Designed for the analysis of volatile fatty acids and phenols Replaces OV-351 Bonded and cross-linked Solvent rinsable Close equivalent to USP Phase G35 We do not recommend the use of water or methanol to rinse DB-FFAP GC columns. Test mix available. See pages 9697 for test mix information. Similar Phases: Stabilwax-DA, HP-FFAP, Nukol, 007-FFAP, BP21, CP-Wax 58 (FFAP) CB, AT-1000, OV-351, CP-FFAP-CB

GC and GC/MS

DB-FFAP Chromatograms
Aspirin and Ibuprofen in Methanol page 421 Organic Acids page 390

DB-FFAP
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.20 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32

25 15 30 30 60 60 15 15 25 30 30 30 50 60 60 60

0.30 0.25 0.25 0.50 0.25 0.50 0.25 1.00 0.50 0.25 0.50 1.00 0.50 0.25 0.50 1.00

40 to 250 40 to 250 40 to 250 40 to 250 40 to 250 40 to 250 40 to 250 40 to 250 40 to 250 40 to 250 40 to 250 40 to 250 40 to 250 40 to 250 40 to 250 40 to 250

128-3222 122-3212 122-3232 122-3233 122-3262 122-3263 123-3212 123-3214 123-3223 123-3232 123-3233 123-3234 123-3253 123-3262 123-3263 123-3264

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265

GC Columns
PEG Columns
DB-FFAP (continued)
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.45 0.45 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53

15 30 10 15 15 30 30 30 30 60

0.85 0.85 1.00 0.50 1.00 0.25 0.50 1.00 1.50 1.00

40 to 250 40 to 250 40 to 250 40 to 250 40 to 250 40 to 250 40 to 250 40 to 250 40 to 250 40 to 250

124-3212* 124-3232* 125-32H2 125-3217 125-3212 125-3231 125-3237 125-3232 125-3233 125-3262

*No special ferrules required for high speed megabore 0.45 mm id columns

HP-FFAP
Nitroterephthalic acid modified polyethylene glycol High polarity Temperature range from 60 to 240/250C (230/240C for 0.53 mm) Designed for the analysis of volatile fatty acids and phenols Replaces OV-351 Bonded and cross-linked Solvent rinsable Close equivalent to USP Phase G35 We do not recommend the use of water or methanol to rinse HP-FFAP GC columns. Similar Phases: Stabilwax-DA, DB-FFAP, Nukol, 007-FFAP, BP21, CP-WAX 58 (FFAP) CB, AT-1000, OV-351, CP-FFAP-CB

GC and GC/MS

HP-FFAP Chromatograms
Acrylates Alcohol Beverage Standard page 410 page 388 Ethoxyethanol page 395

HP-FFAP
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.20 0.20 0.25 0.25 0.32 0.32 0.32 0.32 0.53 0.53 0.53

25 50 15 30 15 25 30 50 10 15 30

0.30 0.30 0.25 0.25 0.25 0.50 0.25 0.50 1.00 1.00 1.00

60 to 240/250 60 to 240/250 60 to 240/250 60 to 240/250 60 to 240/250 60 to 240/250 60 to 240/250 60 to 240/250 60 to 240 60 to 240 60 to 240

19091F-102 19091F-105 19091F-431 19091F-433 19091F-411 19091F-112 19091F-413 19091F-115 19095F-121 19095F-120 19095F-123 www.agilent.com/chem

266

GC Columns
Chiral Columns

Application Specific Stationary Phases


Agilents chemists have developed many columns with unique characteristics designed to solve the most difficult separation problems of a given method. As a result, we offer a comprehensive line of specialty columns for a variety of applications to enhance the standard phase portfolio. From columns for volatiles to pesticides to petrochemical and more Agilent exceeds standard QA/QC procedures for the manufacturing and testing of all of our specialty columns to ensure they meet the stringent demands for their application. These columns offer reliable, accurate results with the shortest run times possible on complex sample lists and matrices.

Chiral Columns
Our proven Cyclodex-B, CycloSil-B and HP-Chiral B offer the chiral analyst a broad range of chiral separations. Although no single column resolves every enantiomeric pair, our no-hassle return policy allows you to try the column for your application and if it doesnt work, simply return it. Recommendations for choosing a chiral column: Contact Technical Support through your local Agilent office for a more specific recommendation Refer to existing applications and literature Choose CycloSil-B as a general purpose column Use HP-Chiral B when using a nitrogen specific detector

GC and GC/MS

CycloSil-B
30% heptakis (2,3-di-O-methyl-6-O-t-butyl dimethylsilyl)--cyclodextrin in DB-1701 Chiral separations without chiral specific derivatization New stationary phase for improved resolution of many chiral separations Ideal for many chiral -lactones and terpenes
Test mix available. See pages 9697 for test mix information. Because CycloSil-B GC columns are not bonded or cross-linked, we do not recommend solvent rinsing.

Similar Phases: LIPODEX C, Rt- DEXm, -DEX 110, -DEX 120

CycloSil-B Chromatograms
Citrus Flavored Carbonated Beverage (Soda) page 387 Rosemary Oil page 387
30% Heptakis (2,3-di-O-methyl-6-O-t-butyldimethylsilyl)-b-cyclodextrin

CycloSil-B
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.25 0.32

30 30

0.25 0.25

35 to 260/280 35 to 260/280

112-6632 113-6632

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267

GC Columns
Chiral Columns

Cyclodex-B
10.5% Beta-Cyclodextrin in DB-1701 Chiral separations without chiral specific derivatization Broad range of resolving potential Excellent peak shape Test mix available. See pages 9697 for test mix information. Because Cyclodex-B GC columns are not bonded or cross-linked, we do not recommend solvent rinsing. Similar Phases: LIPODEX C, Rt- DEXm, -DEX 110, -DEX 120

Cyclodex-B Chromatograms
Menthol page 383

Cyclodex-B
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.25 0.25 0.32 0.53

30 60 30 30

0.25 0.25 0.25 0.50

50 to 230/250 50 to 230/250 50 to 230/250 50 to 210/230

112-2532 112-2562 113-2532 115-2537

GC and GC/MS

HP-Chiral
Beta-cyclodextrin in (35%-Phenyl)-methylpolysiloxane Chiral separations without chiral specific derivatization Phenyl-based polymer provides low bleed and does not interfere with nitrogen-specific detectors. Available in two concentrations of beta-cyclodextrin: 10% and 20% 20% beta-cyclodextrin best choice for initial screening Similar Phases: LIPODEX C, Rt-DEXm, -DEX 110, -DEX 120

HP-Chiral-10
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.25 0.32

30 30

0.25 0.25

30 to 240/250 30 to 240/250

19091G-B133 19091G-B113

HP-Chiral-20
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.25 0.32

30 30

0.25 0.25

30 to 240/250 30 to 240/250

19091G-B233 19091G-B213

268

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GC Columns
Food and Flavors

Food and Flavors


Food and flavor analyses place stringent demands on capillary columns. Samples have many components that are difficult to resolve and column-to-column reproducibility becomes critical. J&W Scientific GC columns are ideal for meeting these needs. Our rigorous quality control specifications and extensive QC testing ensure that the column you buy today will perform just like the column you buy tomorrow. Recommended Columns for Food and Flavors DB-23 for FAMEs and cis- and trans-isomers (page 391) DB-XLB and DB-17ht for triglycerides (page 393) DB-FFAP for organic acids (page 390) DB-1, DB-WAX for fragrance compounds (pages 381382) Microbore (0.1 mm id) DB-1, DB-5 or DB-Wax for fast analysis of fragrances and FAMEs (page 392)

Ultra 1 and Ultra 2


Non-polar Ultra 1: 100% Dimethylpolysiloxane Ultra 2: (5%-Phenyl)-methylpolysiloxane HP-1 and HP-5 with tighter specifications for retention index and capacity factors Intended for use with Sadtler Retention Index Library Four volume reference set that identifies compounds by retention indices alone Catalogs over 2000 compounds by their retention behavior on three HP columns Bonded and cross-linked Solvent rinsable Similar Phases: Ultra1: DB-1, HP-1, SPB-1, CP-Sil 5 CB, Rtx-1, BP-1, 007-1(MS) Ultra 2: DB-5, HP-5, SPB-5, CP-Sil 8 CB, Rtx-5, BP-5, CB-5, 007-5, 2B-5

GC and GC/MS

Ultra 1 and Ultra 2 Chromatograms


Ethylene Glycol Mixture Antiepileptic Drugs Flavor Mixture page 404 page 418 page 383 Tricyclic Antipsychotics Urine Drug Screen page 418 page 416

HP-Ultra 1
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.20 0.20 0.20

12 25 25

0.33 0.11 0.33

-60 to 325/350 -60 to 325/350 -60 to 325/350

19091A-101 19091A-002 19091A-102

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269

GC Columns
Food and Flavors/High Temperature Columns
HP-Ultra 1 (continued)
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.20 0.20 0.32 0.32 0.32 0.32

50 50 25 25 50 50

0.11 0.33 0.17 0.52 0.17 0.52

-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350

19091A-005 19091A-105 19091A-012 19091A-112 19091A-015 19091A-115

HP-Ultra 2
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.20 0.20 0.20 0.20 0.20 0.32 0.32 0.32 0.32

12 25 25 50 50 25 25 50 50

0.33 0.11 0.33 0.11 0.33 0.17 0.52 0.17 0.52

-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350

19091B-101 19091B-002 19091B-102 19091B-005 19091B-105 19091B-012 19091B-112 19091B-015 19091B-115

GC and GC/MS

High Temperature Columns DB-1ht


100% Dimethylpolysiloxane Non-polar Specially processed for extended temperature limit of 400C High temperature, polyimide-coated, fused silica tubing Excellent peak shape and faster elution times for high boilers Bonded and cross-linked Solvent rinsable Test mix available. See pages 9697 for test mix information. Similar Phase: None

DB-1ht
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.25 0.25 0.32 0.32

15 30 15 30

0.10 0.10 0.10 0.10

-60 to 400 -60 to 400 -60 to 400 -60 to 400

122-1111 122-1131 123-1111 123-1131

270

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GC Columns
High Temperature Columns

DB-5ht
(5%-Phenyl)-methylpolysiloxane Non-polar Specially processed for extended temperature limit of 400C High temperature, polyimide-coated, fused silica tubing Excellent peak shape and faster elution times for high boilers Bonded and cross-linked Solvent rinsable Test mix available. See pages 9697 for test mix information. Similar Phase: HT5

DB-5ht Chromatograms
Butter Triglycerides page 393

DB-5ht
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.25 0.25 0.32 0.32

15 30 15 30

0.10 0.10 0.10 0.10

-60 to 400 -60 to 400 -60 to 400 -60 to 400

122-5711 122-5731 123-5711 123-5731

GC and GC/MS

DB-17ht
(50%-Phenyl)-methylpolysiloxane Midpolarity Extended upper temperature limit of 365C High temperature, polyimide-coated, fused silica tubing Excellent peak shape and faster elution times for high boilers Improved resolution for triglycerides Ideal for confirmational analyses Bonded and cross-linked Solvent rinsable Test mix available. See pages 9697 for test mix information. Similar Phases: Rtx-65TG, BPX50, CP-TAP CB

DB-17ht Chromatograms
Butter Triglycerides page 393

DB-17ht
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.25 0.25 0.32 0.32 0.32

15 30 15 30 60

0.15 0.15 0.15 0.15 0.15

40 to 340/365 40 to 340/365 40 to 340/365 40 to 340/365 40 to 340/365

122-1811 122-1831 123-1811 123-1831 123-1861

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271

GC Columns
Life Sciences

Life Sciences
The life sciences offer some difficult challenges to capillary chromatographers. These include complex sample matrices, the necessity for low level detection and the chemically active characteristics of many of the samples. In response to this, Agilent offers a line of columns which are designed specifically for drugs of abuse testing. Recommended Columns for Life Sciences DB-ALC1 and DB-ALC2 for U.S. Blood Alcohol analysis (page 422) DB-ALC2 and HP-Blood Alcohol column for European Blood Alcohol analysis Low-bleed columns for controlled substances DB-35ms for barbiturates (page 418) DB-17ms for hallucinogens (page 420) DB-EVDX for analysis of drugs of abuse DB-624, DB-1, DB-WAX or HP-INNOWax for Residual Solvent analysis (page 424) See pages 341344 for solvent retention data on the DB-624, DB-1 and DB-WAX

DB-ALC1 and DB-ALC2


Reliable blood alcohol analysis Optimized primary and confirmation column pair for U.S. blood alcohol analysis Faster GC run times Improved resolution of key ethanol/acetone peaks Available in 0.32 and 0.53 mm id Bonded and cross-linked Test mix available. See pages 9697 for test mix information. Similar Phases: Rtx-BAC1, Rtx-BAC2

GC and GC/MS

DB-ALC1 and DB-ALC2 Chromatograms


Blood Alcohols (Static Headspace/Split) Blood Pollutants page 422 page 423

DB-ALC1 and DB-ALC2


Phase ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

DB-ALC1 DB-ALC1 DB-ALC2 DB-ALC2

0.32 0.53 0.32 0.53

30 30 30 30

1.80 3.00 1.20 2.00

20 to 260/280 20 to 260/280 20 to 260/280 20 to 260/280

123-9134 125-9134 123-9234 125-9234

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GC Columns
Life Sciences

HP-Blood Alcohol Column


Reliable blood alcohol analysis Excellent confirmation column with DB-ALC2 for method using t-butanol as internal standard

HP-Blood Alcohol Column


ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.32

7.5

20

-60 to 270/290

19091S-510

DB-EVDX
Specially configured and tested for drugs of abuse confirmation DB-1-EVDX: 100% Dimethylpolysiloxane DB-5ms-EVDX: Equivalent to (5%-Phenyl)-methylpolysiloxane DB-17-EVDX: (50%-Phenyl)-methylpolysiloxane Consistent retention and peak shape Test mix includes: caffenine, gluthethimide, lidocaine, phenobarbital, EDDP, methaqualone, methadone, cocaine, desiprmine, carbamazepine Low bleed for GC/MS analysis Bonded and cross-linked Solvent rinsable

DB-EVDX Chromatograms
Anesthetics page 417 Sedative Hypnotics page 420

GC and GC/MS

DB-EVDX
Phase ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

DB-1 EVDX DB-5ms EVDX DB-17 EVDX

0.20 0.20 0.20

25 25 25

0.33 0.33 0.33

-60 to 325/350 -60 to 325/350 40 to 280/300

128-8122 128-8522 128-8722

HP-Fast Residual Solvent Column


Equivalent to USP Phase G43 Thinner film reduces run time by 2.5 times and increases MDL by 2 times compared to standard film thickness used for this method Bonded and cross-linked Similar Phases: DB-624, PE-624, 007-624, 007-502, CP-624, ZB-624

HP Fast Residual Solvents Chromatograms


Fast GC Analysis of USP Solvents page 424

HP Fast Residual Solvents


ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.53

30

1.00

-20 to 260

19095V-420

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273

GC Columns
Pesticides

Pesticides
J&W Scientific low bleed columns are ideal for the analysis of pesticides. Not only do they possess less bleed than a standard polymer which improves the signal to noise ratio and minimum detectable quantities, but they also have higher upper temperature limits which allow for faster run times. See pages 345347 for retention information for 100 common pesticides analyzed on several of our low bleed phases. Agilent also offers several common phases with additional pesticide specific testing to ensure performance for your application. Recommended Columns for Pesticides DB-35ms (P/N 123-3832) and DB-XLB (PN 122-1236) for CLP pesticides, chlorinated herbicides, and EPA Method 508.1 pesticides (pages 349, 351) Also ideal for other dual ECD applications such as 8082 PCBs (Aroclors) and haloacetic acids DB-5ms (PN 122-5532) and DB-35ms (P/N 122-3832) for organophosphorous pesticides (EPA Method 8141A) (pages 356357) HP-5ms for over 550 pesticides using retention time locking software and database See page 317 for Environmental/EPA Method Guide for more column recommendations (pages 317318)

HP-PAS5 and DB-1701P


HP-PAS5: (5%-Phenyl)-methylpolysiloxane Non-polar DB-1701: (14%-Cyanopropyl-phenyl)-methylpolysiloxane Low/midpolarity Exact replacement of HP-PAS1701 Specifically designed and processed for the analysis of organochlorine pesticides ECD tested to assure minimal pesticide breakdown and low ECD bleed Bonded and cross-linked Solvent rinsable Similar Phases: HP-PAS5: DB-5, Ultra-2, SPB-5, CP-Sil 8CB, RSL-200, Rtx-5, BP-5, CB-5, OV-5, 007-2 (MPS-5), SE-52, SE-54, XTI-5, PTE-5, HP-5MS, CC-5, ZB-5 DB-1701P: SPB-1701, CP-Sil 19CB, Rtx-1701, BP-10, CB-1701, OV-1701, 007-1701

GC and GC/MS

274

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GC Columns
Pesticides
HP-PAS5
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.32

25

0.52

-60 to 325/350

19091S-010

DB-1701P
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.25 0.32 0.32 0.53

30 25 30 30

0.25 0.25 0.25 1.00

-20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 260/280

122-7732 123-7722 123-7732 125-7732

DB-608
Specifically designed for the analysis of chlorinated pesticides and PCBs U.S. EPA Methods: 608, 508, 8080 Excellent inertness and recoveries without pesticide breakdown Bonded and cross-linked Solvent rinsable Exact replacement of HP-608 Test mix available. See pages 9697 for test mix information.

GC and GC/MS

Similar Phases: SPB-608, NON-PAKD Pesticide, 007-608

DB-608 Chromatograms
Organochlorine Pesticides page 353

DB-608
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.25 0.32 0.45 0.45 0.53 0.53 0.53

30 30 30 30 15 30 30

0.25 0.50 0.42 0.70 0.83 0.50 0.83

40 to 280/300 40 to 280/300 40 to 260/280 40 to 260/280 40 to 260/280 40 to 260/280 40 to 260/280

122-6832 123-1730 124-6837* 124-1730* 125-1710 125-6837 125-1730

*No special ferrules required for high speed megabore 0.45 mm id columns

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275

GC Columns
Pesticides

J&W Dual Columns


Specific configurations for pesticides, PCBs and volatile priority pollutants No assembly required! Includes two columns, three-way union and 10 meter 0.53 mm id guard column that are connected Matched column dimensions for even sample splitting Leak-free Y connections Performance tested Meets U.S. EPA Method requirements

Dual Columns Chromatograms


Organochlorine Pesticides, DB-5/1701P Dual Column page 355

DB-5/DB-1701P
Phase ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

DB-5 DB-1701P

0.32 0.32

30 30

0.25 0.21

40 to 280/300

009-5007

DB-17/DB-1701P
Phase ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

GC and GC/MS

DB-17 DB-1701P

0.32 0.32

30 30

0.25 0.21

40 to 280/300

009-1707

DB-5/DB-17
Phase ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

DB-5 DB-17

0.32 0.32

30 30

0.25 0.25

40 to 280/300

009-5017

276

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GC Columns
Petroleum

Petroleum
Petroleum applications vary greatly in character. From the noble gases to simulated distillation, Agilent offers a broad range of columns designed to meet the needs of the petroleum/petrochemical chromatographer. Refer to the PLOT column section for columns for the analysis of light gases. Recommended Columns for Petroleum DB-HT Sim Dis for Simulated Distallation (page 441) HP-PONA, DB-5 or HP-1 for PONA and PIANO analysis (page 437438) See PLOT columns for recommendations for light hydrocarbons (page 286) See pages 318322 for ASTM Method Guide for more column recommendations

DB-2887
100% Dimethylpolysiloxane Specifically designed for simulated distillation using ASTM Method D2887 Rapid conditioning, fast run time and low bleed when compared to packed columns Bonded and cross-linked Solvent rinsable Test mix available. See pages 9697 for test mix information. Similar Phases: HP-1, Petrocol EX2887, MXT-2887, MXT-1

GC and GC/MS

DB-2887 Chromatograms
Reference Gas Oil Simulated Distillation page 439 page 439

DB-2887
I.D. Length Film Temp Limits (C) Part No.

0.45 0.53 0.53

10 10 30

2.55 3.00 3.00

-60 to 350 -60 to 350 -60 to 350

124-2814* 125-2814 125-2834

*No special ferrules required for high speed megabore 0.45 mm id columns

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277

GC Columns
Petroleum

DB-HT Sim Dis


100% dimethylpolysiloxane Boiling point phase for high temperature simulated distillation Durable stainless steel tubing 430C upper temperature limit Distillation range of C6 to C110+ Low bleedeven at 430C! Bonded and cross-linked Solvent rinsable Test mix available. See pages 9697 for test mix information. Similar Phases: Petrocol EX2887, CP-SimDist Ultimetal, MXT-2887, Rtx-2887, AC Controls High Temp Sim Dist, AT-2887

DB-HT Sim Dis Chromatograms


n-Paraffin Standard page 441

DB-HT Sim Dis


ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.53

0.15

-60 to 400/430

145-1001

GC and GC/MS

HP-PONA
100% Dimethylpolysiloxane Configured for the analysis of petroleum process products Tested to ensure the resolution of m-xylene from p-xylene and of cyclopentane from 2, 3-dimethylbutane PONA, PIANO High resolution Bonded and cross-linked Solvent rinsable Test mix available. See pages 9697 for test mix information. Similar Phases: Petrocol DH, HP-PONA, SPB-1, 007-1, Rtx-1, MXT-1 Note: 100 psi regulator required to reach optimum carrier gas velocity

278

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GC Columns
Petroleum
HP-PONA Chromatograms
Refinery Gas Sulfur Compounds in Naptha page 431 page 434

HP-PONA and Recommended PONA Columns


Phase ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

HP-PONA HP-1 HP-1

0.20 0.20 0.25

50 50 100

0.50 0.50 0.50

-60 to 325/350 -60 to 325/350 -60 to 325/350

19091S-001 19091Z-205 19091Z-530

DB-Petro
100% Dimethylpolysiloxane Configured for the analysis of petroleum process products PONA, PIANO High resolution Bonded and cross-linked Solvent rinsable Test mix available. See pages 9697 for test mix information. Similar Phases: Petrocol DH, HP-PONA, SPB-1, 007-1, Rtx-1, MXT-1

GC and GC/MS

Note: 100 psi regulator required to reach optimum carrier gas velocity

DB-Petro Chromatograms
Propylene, Butene-1 and Ethylene page 428 Regular Unleaded Gasoline page 440 (California Phase 1) Normal GC Run Unleaded Gasoline PONA Mix as Specified by AFNOR Method #2 page 437 page 438

DB-Petro
I.D. Length Film Temp Limits (C) Part No.

0.20 0.25

50 100

0.50 0.50

-60 to 325/350 -60 to 325/350

128-1056 122-10A6

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279

GC Columns
Semivolatiles

Semivolatiles
Recommended Columns for Semivolatiles HP-5ms for EPA methods 8270 and 525 (page 366) DB-XLB for PCB congeners (pages 361362) HP-5ms or DB-35ms for PAHs DB-5ms or DB-XLB for phenols (page 369) See pages 317318 for Environmental/EPA Method Guide for more column recommendations

DB-Dioxin
Specifically engineered for the analysis of polychlorinated dibenzodioxins (PCDDs) and dibenzofurans (PCDFs) Resolves 2,3,7,8-TCDD and 2,3,7,8-TCDF from all other isomers in one run Low bleed Bonded and cross-linked Solvent rinsable Test mix available. See pages 9697 for test mix information. Similar Phases: SP-2331, 007-23, Rtx-2332, CP-Sil 88 Note: 100 psi regulator required to reach optimum carrier gas velocity

GC and GC/MS

DB-Dioxin Chromatograms
Dioxins and Furans page 360

DB-Dioxin
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.25 0.25 0.32 0.32

60 60 60 60

0.15 0.25 0.15 0.25

40 to 250/270 40 to 250/270 40 to 250/270 40 to 250/270

122-2461 122-2462 123-2461 123-2462

280

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GC Columns
Semivolatiles

DB-5.625
Equivalent to a (5%-Phenyl)-methylpolysiloxane Non-polar Specially processed to exhibit excellent inertness for EPA Semivolatiles Methods 625, 1625, 8270 and CLP protocols* Surpasses EPA performance criteria for semivolatiles Inert for base, neutral and acidic compounds High temperature limit with excellent thermal stability and low bleed Bonded and cross-linked Solvent rinsable
* Pentachlorophenol, 2, 4-Dinitrophenol, Carbazole, and N-Nitrosodiphenylamine used to test response factors.

Test mix available. See pages 9697 for test mix information. Similar Phases: XTI-5, Rtx-5, PTE-5

DB-5.625 Chromatograms
European Red List Volatiles page 377

DB-5.625
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.53

30 30 30 60 30 30 30 30

0.25 0.50 1.00 0.25 0.25 0.50 1.00 1.00

-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 300/320

122-5631 122-5632 122-5633 122-5661 123-5631 123-5632 123-5633 125-5632

GC and GC/MS

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281

GC Columns
Volatiles

Volatiles
The DB-VRX, an industry standard, handles the extensive target list of volatiles in less than half the time as the standard 105m columnswith the fewest coelutions compared to any other commercially available GC column! Another member of the J&W volatiles family is the DB-MTBE which resolves the highly publicized oxygenated fuel additives. Recommended Columns for Volatiles DB-VRX and DB-624 for EPA methods 502.2 and 8021 (pages 373374) DB-VRX (60 m, 0.25 mm id) for GC/MS volatiles methods (page 371) DB-VRX (20 m, 0.18 mm id) for fast GC/MS volatiles analysis using a 5973 MSD (page 372) (Not recommended for Ion Trap MS or older MSDs) DB-MTBE for extended EPA method 8020 (page 347) DB-TPH for the analysis of BTEX and gasoline total petroleum hydrocarbons See pages 317318 for Environmental/EPA Method Guide for more column recommendations

DB-VRX
Unique selectivity engineered for optimum resolution of volatiles analysis: U.S. EPA Methods 502.2, 524.2 and 8260 0.45 mm id columns provide more plates per meter compared to 0.53 mm id columns for the fewest coelutions for GC method (an industry first)** No subambient cooling required to resolve the six gases Fast run time: < 30 minutes for optimum sample throughput < 8 minutes with 0.18 mm id Low polarity Excellent peak shape Bonded and cross-linked Solvent rinsable
** Two coelutions: 1) m- and p-xylene, for which U.S. EPA does not require separation, and 2) 1,1,2,2-tetrachloroethane and o-xylene which are separated by detectors PID and ELCD, respectively. Note to GC/MS analysts: These coeluting compounds have different primary characteristic ions of 83 and 106, respectively.

GC and GC/MS

Test mix available. See pages 9697 for test mix information. Similar Phases: VOCOL, NON-PAKD, Rtx-Volatiles, PE-Volatiles, 007-624, HP-624, CP-624, Rtx-VRX, Rtx-VGC

DB-VRX Chromatograms
EPA Method 504 by GC/MS EPA Volatiles by GC/MS (Split Injector) EPA Method 8021 High Speed VOC EPA 8260 page 376 page 371 page 373 page 372 Unleaded Gasoline page 348

DB-VRX
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.18 0.18 0.25

20 40 30

1.00 1.00 1.40

-10 to 260 -10 to 260 -10 to 260

121-1524 121-1544 122-1534

282

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GC Columns
Volatiles
DB-VRX (continued)
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.25 0.32 0.32 0.45 0.45

60 30 60 30 75

1.40 1.80 1.80 2.55 2.55

- 10 to 260 - 10 to 260 - 10 to 260 - 10 to 260 - 10 to 260

122-1564 123-1534 123-1564 124-1534* 124-1574*

*No special ferrules required for high speed megabore 0.45 mm id columns

DB-624
Specifically designed for the analysis of volatile priority pollutants No cryogenics needed for U.S. EPA Method 502.2 Excellent for U.S. EPA Methods: 501.3, 502.2, 503.1, 524.2, 601, 602, 8010, 8015, 8020, 8240, 8260 Excellent inertness for active compounds Bonded and cross-linked Solvent rinsable Exact replacement of HP-624 Equivalent to USP Phase G43 Test mix available. See pages 9697 for test mix information. Similar Phases: AT-624, Rtx-624, PE-624, 007-624, 007-502, CP-624, ZB-624

GC and GC/MS

DB-624 Chromatograms
1,3-Butadiene Alcohols EPA Method 504 by GC/MS EPA Volatiles GC/MS EPA Method 8021 Esters page 428 page 394 page 376 page 371 page 373 page 402 Ethers Fast VOA Analysis Glycols Halogenated Hydrocarbon Nitrogen Based Solvents Residual Solvents, USP <467> page 403 page 375 page 403 page 405 page 409 page 424

DB-624
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.18 0.20 0.25 0.25 0.32 0.32 0.45 0.45 0.53 0.53 0.53

20 25 30 60 30 60 30 75 30 60 75

1.00 1.12 1.40 1.40 1.80 1.80 2.55 2.55 3.00 3.00 3.00

-20 to 260 -20 to 260 -20 to 260 -20 to 260 -20 to 260 -20 to 260 -20 to 260 -20 to 260 -20 to 260 -20 to 260 -20 to 260

121-1324 128-1324 122-1334 122-1364 123-1334 123-1364 124-1334* 124-1374* 125-1334 125-1364 125-1374

*No special ferrules required for high speed megabore 0.45 mm id columns

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283

GC Columns
Volatiles

HP-VOC
Selectivity engineered for: U.S. EPA Methods 502.2, 524.2 and 8260 Low polarity: slightly more polar than DB-VRX Excellent peak shape Bonded and cross-linked Solvent rinsable Similar Phases: DB-VRX, NON-PAKD, Rtx-Volatiles, PE-Volatiles, 007-624, HP-624, CP-624, Rtx-VRX, Rtx-VGC

HP-VOC
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.20 0.20 0.32 0.32 0.32 0.53 0.53

30 60 60 90 90 90 105

1.12 1.12 1.80 1.00 1.80 3.00 3.00

-60 to 280/290 -60 to 280/290 -60 to 280/290 -60 to 280/290 -60 to 280/290 -60 to 280/290 -60 to 280/290

19091R-303 19091R-306 19091R-316 19091R-219 19091R-319 19095R-429 19095R-420

GC and GC/MS

DB-502.2
Available in 105 meters for volatiles analyses Excellent peak shape Bonded and cross-linked Solvent rinsable Test mix available. See pages 9697 for test mix information. Similar Phases: Rtx-502.2, VOCOL, HP-VOC

DB-502.2
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.25 0.32 0.45 0.45 0.53

60 60 75 105 105

1.40 1.80 2.55 2.55 3.00

0 to 260/280 0 to 260/280 0 to 260/280 0 to 260/280 0 to 260/280

122-1464 123-1464 124-1474* 124-14A4* 125-14A4

*No special ferrules required for high speed megabore 0.45 mm id columns

284

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GC Columns
Volatiles

DB-MTBE
Low polarity stationary phase Resolves MTBE from 2-methylpentane and 3-methylpentane for better quantitation Engineered for purge and trap injection without the need for cryofocusing Bonded and cross-linked Solvent rinsable Test mix available. See pages 9697 for test mix information. Similar Phases: None

DB-MTBE Chromatograms
Methyl-tert-butyl Ether (MTBE) page 347 FID, Extended 8020 Analysis Methyl-tert-butyl Ether (MTBE) page 347 PID, Extended 8020 Analysis

DB-MTBE
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.45 0.53

30 30

2.55 3.00

35 to 260/280 35 to 260/280

124-0034* 125-0034

*No special ferrules required for high speed megabore 0.45 mm id columns

DB-TPH
Specifically designed for the analysis of total petroleum hydrocarbons (TPHs), soil analysis, and LUFT Three analyses in one injection: gas range organics, diesel range organics and motor oil Fast run time Bonded and cross-linked Solvent rinsable Test mix available. See pages 9697 for test mix information. Similar Phase: None

GC and GC/MS

DB-TPH
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.32 0.45

30 30

0.25 1.00

-10 to 320 -10 to 290

123-1632 124-1632*

*No special ferrules required for high speed megabore 0.45 mm id columns

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285

GC Columns
PLOT Columns

PLOT Columns
PLOT columns are ideal for separating compounds that are gases at room temperatures. Agilent Technologies offers a comprehensive line of PLOT columns for analysis of fixed gases, low molecular weight hydrocarbon isomers, volatile polar compounds and reactive analytes such as sulfur gases, amines and hydrides. Our PLOT phases are offered in dimensions from 0.25 to 0.53 mm id, allowing for easy column selection for various detector and system requirements. For GC/MS systems, we offer several small diameter columns with truly bonded and immobilized stationary phases, eliminating potential detector fouling due to particle generation.

PLOT Column Application Recommendations


Column Stationary Phase Typical Applications

HP-PLOT Molesieve

5 molecular sieve zeolite

Permanent and noble gases. Thick and thin films available. Thick film column will resolve argon and oxygen at 35C. Least polar Al2O3 phase. Lowest retention of olefins relative to comparable paraffin. C1 to C8 hydrocarbon isomers. Column of choice for accurate quantitation of dienes, especially propadiene and butadiene from ethylene and propylene streams. Excellent general use Al2O3 column. C1 to C8 hydrocarbon isomers. Best for resolving acetylene from butane and propylene from isobutane. Most polar of the Al2O3 columns. Highest retention of olefins relative to comparable paraffin. Excellent general use Al2O3 column. C1 to C8 hydrocarbon isomers. Best for resolving cyclopropane from propylene. Good stability and recovery from water saturation. C1 to C3 isomers, alkanes to C12, CO2, methane, air/CO, water, oxygenated compounds, sulfur compounds, solvents. More polar than HP-PLOT Q and GS-Q. C1 to C7 hydrocarbons, CO2, methane, air/CO, water, glycol dimethacrylate oxygenates, amines, solvents, alcohols, ketones, aldehydes. C1 to C12 hydrocarbons, CO2, trace-level sulfurs, hydride gases, inorganic gases, halocarbons, SF6, oxygen/nitrogen separation at 80C. C1 to C5 hydrocarbons, CO2, air/CO, trace acetylene in ethylene, methane.

HP-PLOT Al2O3 KCl Aluminum oxide deactivated with KCl

GC and GC/MS

HP-PLOT Al2O3 S

Aluminum oxide deactivated with sodium sulfate

GS-Alumina

Aluminum oxide with proprietary deactivation

HP-PLOT Q

Polystyrene-divinylbenzene

HP-PLOT U

Divinylbenzene/ethylene

GS-GasPro

Proprietary, bonded silica-based

GS-CarbonPLOT

Bonded, monolithic carbon-layer

286

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GC Columns
PLOT Columns

HP-PLOT Al2O3 KCl


Least polar Alumina phase Aluminum oxide deactivated with KCl Standard column choice for light hydrocarbon analysis: C1 to C8 hydrocarbon isomers Low retention of olefins relative to comparable paraffin Excellent for quantitation of dienes, especially propadiene and butadiene from ethylene and propylene streams Recommended phase for many ASTM methods Prefered KCl deactivated Alumina Similar Phases: CP-Al2O3/KCl PLOT, Rt-Alumina PLOT, Alumina PLOT, Al2O3/KCl

HP-PLOT Al2O3 KCl


ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.25 0.32 0.53 0.53

30 50 30 50

5.00 8.00 15.00 15.00

-60 to 200 -60 to 200 -60 to 200 -60 to 200

19091P-K33 19091P-K15 19095P-K23 19095P-K25

GS-Alumina KCl
Least polar Alumina phase Aluminum oxide deactivated with KCl Good choice for light hydrocarbon analysis Good resolution of propadiene and butadiene from ethylene and propylene streams Similar Phases: CP-Al2O3/KCl PLOT, Rt-Alumina PLOT, Alumina PLOT, Al2O3/KCl

GC and GC/MS

GS-Alumina KCl Chromatograms


Impurities in Ethylene Impurities in Propylene page 426 page 427

GS-Alumina KCl
ID (mm) Length (m) Temp Limits (C) Part No.

0.53 0.53

30 50

-60 to 200 -60 to 200

115-3332 115-3352

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287

GC Columns
PLOT Columns

HP-PLOT Al2O3 S
Middle range of polarity for Alumina phases Aluminum oxide deactivated with sodium sulfate Excellent general use column for light hydrocarbon analysis: C1 to C8 hydrocarbon isomers Best for resolving acetylene from butane and propylene from isobutane Similar Phases: CP-Al2O3 PLOT Na2SO4

HP-PLOT Al2O3 S Chromatograms


Ethylene Natural Gas page 426 page 425 Refinery Gas page 431

HP-PLOT Al2O3 S
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.25 0.25 0.32 0.53 0.53 0.53

15 30 50 15 30 50

5.00 5.00 8.00 15.00 15.00 15.00

-60 to 200 -60 to 200 -60 to 200 -60 to 200 -60 to 200 -60 to 200

19091P-S31 19091P-S33 19091P-S15 19095P-S21 19095P-S23 19095P-S25

GC and GC/MS

GS-Alumina
Most polar Alumina phase Aluminum oxide with proprietary deactivation Excellent general use column for light hydrocarbon analysis: C1 to C8 hydrocarbon isomers Separates C1-C4 saturated and unsaturated hydrocarbons Best for resolving cyclopropane from propylene Faster, more efficient and provides more sensitivity than packed equivalents Minimal conditioning time required* Preferred substitution for sodium sulfate deactivated Alumina because of its regenerative nature Note: Alumina columns have a tendency to adsorb water and CO2 which, over time, results in changes in retention time. We use an advanced, proprietary deactivation process which allows for rapid regeneration. Fully water saturated GS-Alumina columns regenerate in 7 hours or less at 200C. Similar Phases: Al2O3/KCl, Al2O3/Na2SO4, Rt-Alumina PLOT, Alumina PLOT

GS-Alumina Chromatograms
1,3-Butadiene Purity page 429 Extended Hydrocarbon Analysis page 430 Propylene page 427

288

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GC Columns
PLOT Columns
GS-Alumina
ID (mm) Length (m) Temp Limits (C) Part No.

0.53 0.53

30 50

-60 to 200 -60 to 200

115-3532 115-3552

HP-PLOT Al2O3 M
Most polar Alumina phase (similar to GS-Alumina) Aluminum oxide deactivated with proprietary deactivation Good general use column for light hydrocarbon analysis: C1 to C8 hydrocarbon isomers Good for resolving acetylene from butane and propylene from isobutane Similar Phases: GS-Alumina

HP-PLOT Al2O3 M
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.32 0.53 0.53

50 30 50

8.00 15.00 15.00

-60 to 200 -60 to 200 -60 to 200

19091P-M15 19095P-M23 19095P-M25

GS-GasPro
GC and GC/MS
Unique bonded PLOT column technology Excellent choice for light hydrocarbons and sulfur gases Retention stability not affected by water Separates CO and CO2 on a single column Ideal PLOT column for GC/MSno particles Similar Phase: CP-Silica PLOT

GS-GasPro Chromatograms
C1 and C2 Halocarbons (Freons) page 380 Extended Hydrocarbon page 430 Analysis GS-Alumina and GasPro Halocarbons page 413 Haloethane page 414 Inorganic Gases page 414 Mercaptans Sulfur Compounds in Propylene (1 ppm) Sulfur Gas Analysis in Light Hydrocarbon Streams page 434 page 434 page 433

GS-GasPro
ID (mm) Length (m) Temp Limits (C) Part No.

0.32 0.32 0.32 0.32

5 15 30 60

-80 to 260/300 -80 to 260/300 -80 to 260/300 -80 to 260/300

113-4302 113-4312 113-4332 113-4362

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289

GC Columns
PLOT Columns

GS-CarbonPLOT
High stability, bonded carbon layer stationary phase Unique selectivity for inorganic and organic gases Extended temperature limit of 360C Similar Phases: Carbopack, CLOT, Carboxen-1006 PLOT, CP-CarboPLOT P7

GS-CarbonPLOT
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.32 0.32 0.32 0.32 0.53 0.53

15 30 30 60 15 30

1.50 1.50 3.00 1.50 3.00 3.00

0 to 360 0 to 360 0 to 360 0 to 360 0 to 360 0 to 360

113-3112 113-3132 113-3133 113-3162 115-3113 115-3133

HP-PLOT Molesieve
GC and GC/MS
A PLOT column for the analysis of permanent gases O2, N2, CO and CH4 resolve in less than 5 minutes Durable molecular sieve 5 coating minimizes baseline spiking and damage to multiport valves Select a thick film for Ar/O2 separation without cryogenic cooling Select thin film HP-PLOT Molesieve columns for routine air monitoring applications Replaces GS-Molesieve Note: Molecular sieve columns will absorb water which, over time, results in changes in retention time. We use an advanced, proprietary deactivation process which allows for rapid regeneration. Fully saturated HP-PLOT Molesieve columns regenerate in 7 hours or less at 200C.

HP-PLOT Molesieve Chromatograms


Noble Gases page 425 Permanent Gases page 425

HP-PLOT MoleSieve
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.32 0.32 0.32 0.32 0.53 0.53 0.53 0.53

15 30 15 30 30 15 30 15

12.00 12.00 25.00 25.00 50.00 25.00 25.00 50.00

-60 to 300 -60 to 300 -60 to 300 -60 to 300 -60 to 300 -60 to 300 -60 to 300 -60 to 300

19091P-MS3 19091P-MS4 19091P-MS7 19091P-MS8 19095P-MS0 19095P-MS5 19095P-MS6 19095P-MS9

290

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GC Columns
PLOT Columns

HP-PLOT Q
Bonded polystyrene-divinylbenzene based column A PLOT column with polarity between Porapak-Q and Porapak-N Excellent column for C1 to C3 isomers and Alkanes to C12, CO2, methane, air/CO, oxygenated compounds, sulfur compunds and solvents A PLOT column to replace packed gas-solid columns Separates ethane, ethylene and ethyne (acetylene) Improved resolution in less time than conventional packed columns Minimal conditioning time required1 hour Preferred Q column due to its robust nature Similar Phases: CP PoraPLOT Q, CP PoraPLOT Q-HT, Rt-QPLOT, SupelQ PLOT, GS-Q

HP-PLOT Q Chromatograms
Baseline Resolution of Air/CO, page 425 CO2, and Methane in Natural Gas Sample Ethylene Oxide page 436 Synthetic Standard Oxygenates Separation of Refinery Gas Sample page 436 page 431

HP-PLOT Q
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.32 0.32 0.53 0.53

15 30 15 30

20.00 20.00 40.00 40.00

-60 to 270/290 -60 to 270/290 -60 to 270/290 -60 to 270/290

19091P-QO3 19091P-QO4 19095P-QO3 19095P-QO4

GC and GC/MS

Particle Traps for Use with PLOT Columns


Though highly stabilized, it is impossible to guarantee that no particles will dislodge from the column wall. When used in valve-switching applications, the use of a particle trap can prevent scarring of the rotors.

Particle Traps
ID (mm) Length (m) Part No.

0.32 0.53

2.5 2.5

5181-3351 5181-3352

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291

GC Columns
PLOT Columns

GS-Q
Porous divinylbenzene homopolymer A PLOT column with polarity between Porapak-Q and Porapak-N Separates ethane, ethylene and ethyne (acetylene) Not recommended for quantification of polar compounds Minimal conditioning time required1 hour Similar Phases: CP PoraPLOT Q, CP PoraPLOT Q-HT, Rt-QPLOT, SupelQ PLOT, HP-Q PLOT Note: GS-Q has been proven useful for separations of various gases including freons and sulfides (except for SO2). Although GS-Q is not suitable for quantitating water or alcohols, aqueous injections will not harm the column.

GS-Q Chromatograms
Sulfur Gas Analysis in Light Hydrocarbon Streams page 433

GS-Q
ID (mm) Length (m) Temp Limits (C) Part No.

0.32 0.32 0.32 0.53 0.53 0.53 0.53

15 25 30 10 15 25 30

-60 to 250 -60 to 250 -60 to 250 -60 to 250 -60 to 250 -60 to 250 -60 to 250

113-3412 113-3422 113-3432 115-34H2 115-3412 115-3422 115-3432

GC and GC/MS

HP-PLOT U
Bonded divinylbenzene/ethylene glycol dimethacrylate More polar than HP-PLOT Q Excellent column for C1 to C7 hydrocarbons, CO2, methane, air/CO, water, oxygenates, amines, solvents, alcohols, ketones, and aldehydes Improved resolution in less time than conventional packed columns Similar Phases: PoraPlot U, RTU PLOT

HP-PLOT U
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.32 0.32 0.53 0.53

15 30 15 30

10 10 20 20

-60 to 190 -60 to 190 -60 to 190 -60 to 190

19091P-UO3 19091P-UO4 19095P-UO3 19095P-UO4

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GC Columns
Metal Columns

Metal Columns DB-ProSteel Series Stainless Steel GC Columns


Excellent inertness Virtually unbreakable Available in a wide variety of stationary phases Bonded and cross-linked Ideal for high temperature analysis and process applications Our easy to handle DB-ProSteel metal columns are deactivated with a new formula (this is not glass lined steel) to provide inertness which truly rivals fused silica. DB-ProSteel metal columns can be custom wound upon request for small GC ovens. Several of our most popular bonded phases are available in metal. DB-ProSteel GC columns have the same outer diameter as standard Megabore (0.53 mm id), so no special ferrules are required.

DB-ProSteel
Phase ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

DB-PS1 DB-PS1 DB-PS1 DB-PS1 DB-PS1 DB-PS1 DB-PS1 DB-PS1 DB-PS5 DB-PS624 DB-PS2887 DB-PSWAX DB-PSWAX

0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53

15 15 15 30 30 30 30 30 30 30 10 15 30

0.15 1.50 0.50 0.15 1.50 3.00 5.00 0.50 1.50 3.00 3.00 1.00 1.00

-60 to 340/360 -60 to 300/320 -60 to 300/320 -60 to 340/360 -60 to 300/320 -60 to 260/280 -60 to 260/280 -60 to 300/320 -60 to 300/320 -20 to 260 -60 to 350 20 to 230/240 20 to 230/240

145-1011 145-1012 145-1017 145-1031 145-1032 145-1034 145-1035 145-1037 145-5032 145-1334 145-2814 145-7012 145-7032

GC and GC/MS

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293

GC Columns
Non-Bonded Phases

Non-Bonded Phases
Whenever possible Agilent recommends the use of bonded and cross-linked columns. Bonded columns are more rugged, will have longer lifetimes and can be solvent rinsed. However, Agilent recognizes that some methods have been developed on these non-bonded phases and therefore maintains these columns to support established methods.

HP-101
100% polydimethylsiloxane Similar Phases: OV-101, SP 200, DB-1, HP-1 Because HP-101 columns are not bonded or cross-linked, we do not recommend solvent rinsing.

HP-101
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.20 0.20 0.20 0.32 0.32

12 25 50 25 50

0.25 0.20 0.20 0.30 0.30

0 to 280 0 to 280 0 to 280 0 to 280 0 to 280

19091-60010 19091Y-102 19091Y-105 19091Y-012 19091Y-015

GC and GC/MS

HP-1 is the recommended bonded replacement for HP-101, see page 235

HP-17
50% Phenyl and 50% Methyl siloxane Similar Phases: OV-17, SP-2250, DB-17, HP-50+ Because the HP-17 is not bonded or cross-linked, we do not recommend solvent rinsing.

HP-17
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.53

10

2.00

25 to 260/280

19095L-121

294

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GC Columns
Non-Bonded Phases

Carbowax 20M and HP-20M


Polyethylene glycol, MW 20,000 Equivalent to USP Phase G16 Similar Phases: DB-WAX, HP-INNOWax, DB-WAXetr Because the Carbowax 20M and HP-20M are not bonded or cross-linked, we do not recommend solvent rinsing.

Carbowax 20M
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.25 0.25 0.32 0.32

30 60 30 60

0.25 0.25 0.25 0.25

60 to 220/240 60 to 220/240 60 to 220/240 60 to 220/240

112-2032 112-2062 113-2032 113-2062

DB-WAX is the recommended bonded replacement for HP-20M, see page 260

HP-20M
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.20 0.20 0.32 0.32 0.53 0.53

25 50 25 50 10 30

0.10 0.10 0.30 0.30 1.33 1.33

60 to 220 60 to 220 60 to 220 60 to 220 60 to 220 60 to 220

19091W-102 19091W-105 19091W-012 19091W-015 19095W-121 19095W-123

GC and GC/MS

DB-WAX is the recommended bonded replacement for HP-20M, see page 260

OV-351
Nitroterephthalic acid modified polyethylene glycol Equivalent to USP Phase G35 Similar Phases: DB-FFAP and HP-FFAP Because the OV-351 is not bonded or cross-linked, we do not recommend solvent rinsing.

OV-351
I.D. 0.25 0.32 Length 30 30 Film 0.25 0.25 Temp Limits (C) 60 to 220/240 60 to 220/240 Part No. 112-5132 113-5132

DB-FFAP is the recommended bonded replacement for OV-351, see page 265

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295

GC Columns
Non-Bonded Phases

CAM
Base deactivated polyethylene glycol Specifically designed for amine analysis Excellent peak shape for primary amines Replaces HP-Basicwax Test mix available. See pages 9697 for test mix information. Similar Phases: CAM: Stabilwax-DB, Carbowax Amine Because the CAM is not bonded or cross-linked, we do not recommend solvent rinsing.

CAM Chromatograms
Amines in Water Primary Amines page 397 page 396

CAM
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.53 0.53 0.53

15 30 30 60 15 30 30 15 30 60

0.25 0.25 0.50 0.25 0.25 0.25 0.50 1.00 1.00 1.00

60 to 220/240 60 to 220/240 60 to 220/240 60 to 220/240 60 to 220/240 60 to 220/240 60 to 220/240 60 to 200/220 60 to 200/220 60 to 200/220

112-2112 112-2132 112-2133 112-2162 113-2112 113-2132 113-2133 115-2112 115-2132 115-2162

GC and GC/MS

296

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GC Columns
Non-Bonded Phases

DX-1, DX-3 and DX-4


DX-1: 90% Dimethylpolysiloxane 10% Polyethylene Glycol DX-3: 50% Dimethylpolysiloxane 50% Polyethylene Glycol DX-4: 15% Dimethylpolysiloxane 85% Polyethylene Glycol Test mix available. See pages 9697 for test mix information. Because DX series GC columns are not bonded and cross-linked, we do not recommend solvent rinsing.

DX-1
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.25 0.25 0.32

15 30 30

0.25 1.00 1.00

50 to 250/270 50 to 250/270 50 to 250/270

122-6112 122-6133 123-6133

DX-3
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.25 0.25 0.32

15 30 30

0.25 0.25 0.25

50 to 250/270 50 to 250/270 50 to 250/270

122-6312 122-6332 123-6332

GC and GC/MS

DX-4
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.25 0.25 0.25 0.32 0.32 0.32

15 30 60 15 30 60

0.25 0.25 0.25 0.25 0.25 0.25

50 to 250/270 50 to 250/270 50 to 250/270 50 to 250/270 50 to 250/270 50 to 250/270

122-6412 122-6432 122-6462 123-6412 123-6432 123-6462

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297

GC and GC/MS
SE Series GC Columns

SE Series GC Columns
SE-30: 100% Dimethylpolysiloxane SE-54: (5%-Phenyl)(1%-Vinyl)-methylpolysiloxane Test mix available. See pages 9697 for test mix information. Because SE series GC columns are not bonded or cross-linked, we do not recommend solvent rinsing.

SE-30
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.25 0.32 0.32

30 30 60

0.25 0.25 0.25

0 to 325/350 0 to 325/350 0 to 325/350

112-3032 113-3032 113-3062

DB-1 is a bonded replacement for SE-30, see page 231

SE-54
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.25 0.25 0.32 0.32

30 60 15 30

0.25 0.25 0.25 0.25

0 to 325/350 0 to 325/350 0 to 325/350 0 to 325/350

112-5432 112-5462 113-5412 113-5432

GC and GC/MS

DB-5 is a bonded replacement for SE-54, see page 238

298

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GC Columns
Custom Columns

Custom Columns
Even though we offer over a thousand readily available columns, Agilent recognizes that sometimes you need something a little out of the ordinary. Thats why we developed our Custom Column Shop. So if you cant find what you are looking for in our standard order guides we will design, build and test capillary GC columns for customers to meet your needs. We can create columns with non-standard lengths or unusual film thickness. Well connect columns together in series or as dual columns. We also recognize that sometimes customers have specific column performance requirements for their applications that might not be met with standard test mixes. As a result we can also custom-test your columns with your desired test mixture and test conditions to meet your specific performance requirements. DuraGuard columns with an integrated guard column. Most phases can be manufactured with a built-in guard column which means you get the advantages of a guard column without the union. Custom columns are ordered under the part number 100-2000. Be sure to provide the details of your desired custom service or column including phase, length, id, and film thickness. Contact your local Agilent office or your authorized Agilent Distributor to receive a quote for your Custom Column needs.

GC and GC/MS

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299

GC Columns
GC Columns on 5 in. Cage

Columns for the 6850 GC


GC capillary columns are typically wound on 7 in. cages that fit into standard-sized GCs like the 6890. The 6850 GC has a smaller oven which requires the use of columns wound on a smaller cage diameter. The 5 in. cage configurations listed here are available as standard products. If you require a column for your 6850 that is not listed below the column, simply order part number 100-2000 and specify: the standard cage part number, on 5 in. cage. or the phase and dimensions (length, internal diameter, film thickness), on 5 in. cage. The price of the 5 in. cage column will be the same as the same column on a standard cage.

CAM - 5 in. cage


ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.25 0.32

30 30

0.50 0.25

60 to 220/240 60 to 220/240

112-2133E 113-2132E

DB-1 - 5 in. cage

GC and GC/MS

ID (mm)

Length (m)

Film (m)

Temp Limits (C)

Part No.

0.10 0.10 0.10 0.25 0.25 0.32 0.32 0.32 0.53 0.53 0.53 0.53

10 10 40 30 30 30 60 60 15 15 30 30

0.10 0.40 0.40 0.25 1.00 1.00 3.00 5.00 0.15 1.50 3.00 5.00

-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 280/300 -60 to 280/300 -60 to 340/360 -60 to 300/320 -60 to 260/280 -60 to 260/280

127-1012E 127-1013E 127-1043E 122-1032E 122-1033E 123-1033E 123-1064E 123-1065E 125-1011E 125-1012E 125-1034E 125-1035E

DB-1ms - 5 in. cage


ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.10

10

0.40

-60 to 340/360

127-0113E

300

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GC Columns
GC Columns on 5 in. Cage
DB-5 - 5 in. cage
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.25 0.32 0.32 0.32 0.53 0.53

30 15 30 60 30 60

0.25 0.25 0.25 1.00 1.50 1.50

-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 300/320 -60 to 300/320

122-5032E 123-5012E 123-5032E 123-5063E 125-5032E 125-5062E

DB-5ms - 5 in. cage


ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.25 0.32

30 30

1.00 0.25

-60 to 325/350 -60 to 325/350

122-5533E 123-5532E

DB-17 - 5 in. cage


ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.32

30

0.25

40 to 280/300

123-1732E

DB-225ms - 5 in. cage

GC and GC/MS

ID (mm)

Length (m)

Film (m)

Temp Limits (C)

Part No.

0.25

15

0.25

40 to 220/240

122-2212E

DB-624 - 5 in. cage


ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.25 0.25 0.32 0.32 0.53 0.53

30 60 30 60 30 75

1.40 1.40 1.80 1.80 3.00 3.00

-20 to 260 -20 to 260 -20 to 260 -20 to 260 -20 to 260 -20 to 260

122-1334E 122-1364E 123-1334E 123-1364E 125-1334E 125-1374E

DB-1301 - 5 in. cage


ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.25 0.32

60 30

1.00 1.00

-20 to 280/300 -20 to 280/300

122-1363E 123-1333E

DB-1701 - 5 in. cage


ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.25 0.32 0.32 0.32 0.53

30 30 30 60 30

0.25 0.25 1.00 1.00 1.00

-20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 280/300 -20 to 260/280

122-0732E 123-0732E 123-0733E 123-0763E 125-0732E

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301

GC Columns
GC Columns on 5 in. Cage
DB-1701P - 5 in. cage
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.32

30

0.25

-20 to 280/300

123-7732E

DB-FFAP - 5 in. cage


ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.32

30

0.25

40 to 250

123-3232E

DB-MTBE - 5 in. cage


ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.53

30

3.00

35 to 260/280

125-0034E

DB-Petro - 5 in. cage


ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.25

100

0.50

-60 to 325/350

122-10A6E

DB-PS1 - 5 in. cage


ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.53

30

3.00

-60 to 260/280

145-1034E

GC and GC/MS

DB-VRX - 5 in. cage


ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.25

60

1.40

-10 to 260

122-1564E

DB-WAX - 5 in. cage


ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.10 0.10 0.25 0.25 0.32 0.32 0.32 0.53

10 10 30 30 30 30 60 30

0.10 0.20 0.25 0.50 0.25 0.50 0.50 1.00

20 to 250/260 20 to 240/250 20 to 250/260 20 to 240/250 20 to 250/260 20 to 240/250 20 to 240/250 20 to 230/240

127-7012E 127-7013E 122-7032E 122-7033E 123-7032E 123-7033E 123-7063E 125-7032E

GS-Q - 5 in. cage


ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.53

30

-60 to 250

115-3432E

302

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GC Columns
GC Columns on 5 in. Cage
HP-1 - 5 in. cage
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.20 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.53

25 30 30 60 60 100 15 15 25 25 30 30 30 30 50 60 5 5 10 15 15 15 30 30 30 30

0.33 0.25 1.00 0.25 1.00 0.50 0.25 1.00 0.17 0.52 0.25 1.00 3.00 5.00 0.52 1.00 0.15 2.65 2.65 0.15 3.00 5.00 1.50 2.65 3.00 5.00

-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 260/280 -60 to 260/280 -60 to 325/350 -60 to 325/350 -60 to 320/360 -60 to 260/280 -60 to 260/280 -60 to 320/360 -60 to 260/280 -60 to 260/280 -60 to 300/320 -60 to 260/280 -60 to 260/280 -60 to 260/280

19091Z-102E 19091Z-433E 19091Z-233E 19091Z-436E 19091Z-236E 19091Z-530E 19091Z-411E 19091Z-211E 19091Z-012E 19091Z-112E 19091Z-413E 19091Z-213E 19091Z-513E 19091Z-713E 19091Z-115E 19091Z-216E

GC and GC/MS

19095Z-220E 19095S-100E 19095Z-121E 19095Z-221E 19095Z-421E 19095Z-621E 19095Z-323E 19095Z-123E 19095Z-423E 19095Z-623E

HP-1ms - 5 in. cage


ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.25 0.25 0.25

30 30 60

0.10 0.25 0.25

-60 to 325/350 -60 to 325/350 -60 to 325/350

19091S-833E 19091S-933E 19091S-936E

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303

GC Columns
GC Columns on 5 in. Cage
HP-5 - 5 in. cage
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.20 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.53 0.53 0.53 0.53

25 30 60 60 15 25 30 50 10 30 30 30

0.33 0.25 0.25 1.00 0.25 0.52 0.25 0.52 2.65 0.88 1.50 2.65

-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 260/280 -60 to 300/320 -60 to 300/320 -60 to 260/280

19091J-102E 19091J-433E 19091J-436E 19091J-236E 19091J-411E 19091J-112E 19091J-413E 19091J-115E 19095J-121E 19095J-023E 19095J-323E 19095J-123E

HP-5ms - 5 in. cage


ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.20

25 30 30 30

0.33 0.10 0.25 1.00

-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350

19091S-102E 19091S-333E 19091S-433E 19091S-233E

GC and GC/MS

0.25 0.25 0.25

HP-50+ - 5 in. cage


ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.32 0.32 0.53

30 30 30

0.25 0.50 1.00

40 to 280/300 40 to 280/300 40 to 260/280

19091L-413E 19091L-113E 19095L-023E

HP-101 - 5 in. cage


ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.20 0.20

12 25

0.25 0.20

-60 to 280 -60 to 280

19091-60010E 19091Y-102E

HP Blood Alcohol Column - 5 in. cage


ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.32

7.5

20.00

-60 to 270/290

19091S-510E

HP Fast GC Residual Solvents - 5 in. cage


ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.53

30

1.00

-20 to 260

19095V-420E

304

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GC Columns
GC Columns on 5 in. Cage
HP-FFAP - 5 in. cage
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.20 0.25 0.32 0.32 0.53 0.53

50 30 25 50 15 30

0.30 0.25 0.50 0.50 1.00 1.00

60 to 240/250 60 to 240/250 60 to 240/250 60 to 240/250 60 to 240 60 to 240

19091F-105E 19091F-433E 19091F-112E 19091F-115E 19095F-120E 19095F-123E

HP-INNOWax - 5 in. cage


ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.53 0.53

30 30 60 30 30 30 60 60 15 30

0.25 0.50 0.25 0.15 0.25 0.50 0.25 0.50 1.00 1.00

40 to 260/270 40 to 260/270 40 to 260/270 40 to 260/270 40 to 260/270 40 to 260/270 40 to 260/270 40 to 260/270 40 to 240/250 40 to 240/250

19091N-133E 19091N-233E 19091N-136E 19091N-013E 19091N-113E 19091N-213E 19091N-116E 19091N-216E 19095N-121E 19095N-123E

GC and GC/MS

HP-PLOT Al2O3 KCl - 5 in. cage


ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.32

50

8.00

-60 to 200

19091P-K15E

HP-PLOT Al2O3 M - 5 in. cage


ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.53

50

15.00

-60 to 200

19095P-M25E

HP-PLOT Al2O3 S - 5 in. cage


ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.32 0.53

50 50

8.00 15.00

-60 to 200 -60 to 200

19091P-S15E 19095P-S25E

HP-PLOT Molesieve - 5 in. cage


ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.32 0.53 0.53

30 30 30

12.00 25.00 50.00

-60 to 300 -60 to 300 -60 to 300

19091P-MS4E 19095P-MS6E 19095P-MS0E

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305

GC Columns
GC Columns on 5 in. Cage
HP-PLOT Q - 5 in. cage
ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.32 0.53 0.53

25 15 25

20 40 40

-60 to 270/290 -60 to 270/290 -60 to 270/290

19091P-QO4E 19095P-QO3E 19095P-QO4E

HP-PLOT U - 5 in. cage


ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.32 0.53

30 30

10 20

-60 to 190 -60 to 190

19091P-UO4E 19095P-UO4E

HP-PONA - 5 in. cage


ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.20

50

0.50

-60 to 325/350

19091S-001E

Ultra 1 - 5 in. cage


ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.20

25

0.33

-60 to 325/350

19091A-102E

GC and GC/MS

Ultra 2 - 5 in. cage


ID (mm) Length (m) Film (m) Temp Limits (C) Part No.

0.20 0.32

25 25

0.33 0.17

-60 to 325/350 -60 to 325/350

19091B-102E 19091B-012E

Cage for 68550


Description 5 in. cage for 6850, 13 cm/5 in. od Part No. 19091-80060

306

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GC Columns
Quality Control Specifications

The Tightest QC Specifications in the Industry


Highest level of column-to-column reproducibility Decreased column bleed for increased sensitivity All J&W Scientific columns are individually tested and must live up to our stringent QC requirements We take column manufacturing seriously and strive to make the best columns possible. And to prove it, QC for column bleed, retention index window, and plates per meter are published for our most popular phases and column configurations. The individual bleed specifications represent the difference, in pico amperes of the FID response, measured at the upper temperature limit of the column and the isothermal test temperature (generally 110C to 125C). We strive for the lowest bleed GC columns in the industry. The specifications for RI represent our narrow retention index windows. A small number means you get the highest level of column-to-column reproducibility. Plates per meter is a measurement of the resolving potential for a GC column. We publish the minimum plate specifications that a column must have before it can become a J&W capillary column.

Quality Control Specifications


ID (mm) DB-1 0.18 0.18 0.18 0.18 0.18 0.20 0.20 0.20 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 10 10 20 20 40 12 25 50 15 15 15 30 30 30 60 60 60 15 15 15 25 30 30 30 60 60 60 0.18 0.40 0.18 0.40 0.40 0.33 0.33 0.33 0.10 0.25 1.00 0.10 0.25 1.00 0.10 0.25 1.00 0.10 0.25 1.00 0.52 0.10 0.25 1.00 0.10 0.25 1.00 4 6 6 6 8 4 6 8 4 4 10 6 6 15 7 7 15 4 4 10 15 6 6 15 8 8 15 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 5,150 4,850 5,150 4,850 4,600 4,800 4,800 4,650 3,900 3,900 3,500 3,900 3,900 3,500 3,700 3,700 3,300 3,100 3,100 3,000 3,100 3,100 3,100 3,000 3,000 3,000 2,800 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 121-1012 121-1013 121-1022 121-1023 121-1043 128-1012 128-1022 128-1052 122-1011 122-1012 122-1013 122-1031 122-1032 122-1033 122-1061 122-1062 122-1063 123-1011 123-1012 123-1013 123-1026 123-1031 123-1032 123-1033 123-1061 123-1062 123-1063 Length (m) Film Thickness (m) Maximum Allowable Bleed (pA) RI Tolerance (+/- I) Minimum Plates/ Meter Temperature Limits (C)

GC and GC/MS

Part No.

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307

GC Columns
Quality Control Specifications
Quality Control Specifications (continued)
ID (mm) DB-1ms 0.20 0.20 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 DB-5 0.18 0.18 0.18 0.18 0.18 0.20 0.20 0.20 0.25 0.25 0.25 10 10 20 20 40 12 25 50 15 15 15 30 30 30 60 60 60 15 15 15 25 30 30 30 50 60 60 60 0.18 0.40 0.18 0.40 0.40 0.33 0.33 0.33 0.10 0.25 1.00 0.10 0.25 1.00 0.10 0.25 1.00 0.10 0.25 1.00 0.52 0.10 0.25 1.00 1.00 0.10 0.25 1.00 4 8 7 8 15 5 7 10 4 5 10 6 6 15 8 10 15 6 6 10 12 6 8 15 15 10 10 15 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 5,150 5,150 5,150 5,150 4,850 4,600 4,600 4,300 3,900 3,900 3,500 3,900 3,900 3,500 3,700 3,700 3,500 3,100 3,100 2,800 3,100 3,100 3,100 2,800 2,800 3,100 2,950 2,800 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 121-5012 121-5013 121-5022 121-5023 121-5043 128-5012 128-5022 128-5052 122-5011 122-5012 122-5013 122-5031 122-5032 122-5033 122-5061 122-5062 122-5063 123-5011 123-5012 123-5013 123-5026 123-5031 123-5032 123-5033 123-5053 123-5061 123-5062 123-5063 12 25 15 30 30 60 15 30 30 60 0.33 0.33 0.25 0.10 0.25 0.25 0.25 0.10 0.25 0.25 3 4 3 3 4 7 3.5 3 4 8 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 4,800 4,800 3,900 3,900 3,900 3,700 3,100 3,100 3,100 3,000 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 -60 to 340/360 128-0112 128-0122 122-0112 122-0131 122-0132 122-0162 123-0112 123-0131 123-0132 123-0162 Length (m) Film Thickness (m) Maximum Allowable Bleed (pA) RI Tolerance (+/- I) Minimum Plates/ Meter Temperature Limits (C) Part No.

GC and GC/MS

0.25 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 DB-5ms 0.18 0.18 0.20 0.20 0.20 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25

20 40 12 25 50 15 15 15 30 30 30 30 60 60 60

0.18 0.18 0.33 0.33 0.33 0.10 0.25 1.00 0.10 0.25 1.00 0.50 0.10 0.25 1.00

3 3 3 4 5 2.5 3 6 3 4 6 5 6 8.5 12

0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5

4,900 4,900 4,600 4,600 4,300 3,700 3,700 3,500 3,700 3,700 3,500 3,700 3,500 3,700 3,500

-60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350

121-5522 121-5542 128-5512 128-5522 128-5552 122-5511 122-5512 122-5513 122-5531 122-5532 122-5533 122-5536 122-5561 122-5562 122-5563

308

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GC Columns
Quality Control Specifications
Quality Control Specifications (continued)
ID (mm) 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 DB-5ms ITD 0.25 DB-WAX 0.18 0.18 0.18 0.18 0.18 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.32 0.32 DB-17 0.18 0.18 0.18 0.18 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.32 0.32 10 10 20 20 15 15 15 30 30 30 60 15 15 15 30 30 30 0.18 0.30 0.18 0.30 0.15 0.25 0.50 0.15 0.25 0.50 0.25 0.15 0.25 0.50 0.15 0.25 0.50 5 10 10 15 10 10 15 10 10 15 15 10 10 15 10 10 15 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 4,850 4,500 4,850 3,950 3,700 3,700 2,900 3,700 3,700 2,900 3,500 3,000 3,000 2,650 3,000 3,000 2,650 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 40 to 280/300 121-1712 121-1713 121-1722 121-1723 122-1711 122-1712 122-1713 122-1731 122-1732 122-1733 122-1762 123-1711 123-1712 123-1713 123-1731 123-1732 123-1733 10 10 20 20 40 15 15 15 30 30 30 60 60 60 15 15 30 30 30 60 60 0.18 0.30 0.18 0.30 0.30 0.15 0.25 0.50 0.15 0.25 0.50 0.15 0.25 0.50 0.25 0.50 0.15 0.25 0.50 0.25 0.50 10 15 15 15 20 15 20 20 15 20 20 15 30 30 20 20 15 20 20 30 30 2.5 2.0 2.5 2.0 2.0 2.0 2.0 2.5 2.0 2.0 2.5 2.5 2.5 2.5 2.0 2.0 2.0 2.0 2.0 2.0 2.0 4,600 4,600 4,600 4,600 4,300 3,600 3,500 3,300 3,600 3,500 3,300 3,600 3,500 3,300 3,000 2,800 3,000 3,000 2,800 2,800 2,800 20 to 250/260 20 to 240/260 20 to 250/260 20 to 240/260 20 to 240/260 20 to 250/260 20 to 250/260 20 to 240/260 20 to 250/260 20 to 250/260 20 to 240/260 20 to 250/260 20 to 250/260 20 to 240/260 20 to 250/260 20 to 240/260 20 to 250/260 20 to 250/260 20 to 240/260 20 to 250/260 20 to 240/260 121-7012 121-7013 121-7022 121-7023 121-7043 122-7011 122-7012 122-7013 30 0.25 4 0.5 3,700 -60 to 325/350 122-4132 Length (m) 15 15 15 25 30 30 30 30 60 60 60 Film Thickness (m) 0.10 0.25 1.00 0.52 0.10 0.25 0.50 1.00 0.10 0.25 1.00 Maximum Allowable Bleed (pA) 2.5 3.5 6 5 3 4 5 6 6 9 12 RI Tolerance (+/- I) 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Minimum Plates/ Meter 3,100 3,000 2,800 3,000 3,100 3,000 3,000 2,800 2,800 2,800 2,800 Temperature Limits (C) -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 -60 to 325/350 Part No. 123-5511 123-5512 123-5513 123-5526 123-5531 123-5532 123-5536 123-5533 123-5561 123-5562 123-5563

DB-5ms (continued)

GC and GC/MS

122-7031 122-7032 122-7033 122-7061 122-7062 122-7063 123-7012 123-7013 123-7031 123-7032 123-7033 123-7062 123-7063

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309

GC Columns
Quality Control Specifications
Quality Control Specifications (continued)
ID (mm) DB-17ms 0.18 0.25 0.25 0.25 0.32 0.32 DB-35ms 0.20 0.25 0.25 0.25 0.32 DB-200 0.25 0.25 0.25 0.32 0.32 DB-225ms 0.25 0.25 0.25 15 30 60 15 30 0.25 0.25 0.25 0.25 0.25 15 15 30 15 15 3 3 3 3 3 3,300 3,300 3,300 2,625 2,625 40 to 240 40 to 240 40 to 240 40 to 240 40 to 240 122-2912 122-2932 122-2962 123-2912 123-2932 15 30 30 30 30 0.25 0.25 0.50 0.25 0.50 10 10 15 10 15 1 1 1 1 1 3,500 3,500 3,500 2,800 2,800 30 to 300/320 30 to 300/320 30 to 300/320 30 to 300/320 30 to 300/320 122-2012 122-2032 122-2033 123-2032 123-2033 25 15 30 60 30 0.33 0.25 0.25 0.25 0.25 5 5.5 6 13 7.5 1 1 1 1 1 4,300 3,500 3,500 3,500 2,800 50 to 340/360 50 to 340/360 50 to 340/360 50 to 340/360 50 to 340/360 128-3822 122-3812 122-3832 122-3862 123-3832 20 15 30 60 15 30 0.18 0.25 0.25 0.25 0.25 0.25 5.5 5.5 5.5 11 7 7 1.5 1.0 1.0 1.0 1.0 1.0 3,700 3,700 3,700 3,500 2,800 2,800 40 to 320/340 40 to 320/340 40 to 320/340 40 to 320/340 40 to 320/340 40 to 320/340 121-4722 122-4712 122-4732 122-4762 123-4712 123-4732 Length (m) Film Thickness (m) Maximum Allowable Bleed (pA) RI Tolerance (+/- I) Minimum Plates/ Meter Temperature Limits (C) Part No.

GC and GC/MS

0.32 0.32 DB-XLB 0.18 0.18 0.20 0.20 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32

20 30 12 25 15 15 30 30 30 30 60 30 30 30 60

0.18 0.18 0.33 0.33 0.10 0.25 0.10 0.25 1.00 0.50 0.25 0.25 1.00 0.50 0.25

6 6 5 6 4 6 4 6 9 9.5 12 7.5 13 9.5 15

1 1 1 1 1 1 1 1 1 1 1 1 1 1 1

5,150 5,150 4,600 4,600 3,700 3,900 3,700 3,900 3,500 3,700 3,700 3,000 2,800 3,100 3,000

30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360 30 to 340/360

121-1222 121-1232 128-1212 128-1222 122-1211 122-1212 122-1231 122-1232 122-1233 122-1236 122-1262 123-1232 123-1233 123-1236 123-1262

310

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GC Columns
Fused Silica Tubing

Fused Silica Tubing


Deactivated Tubing
Deactivated tubing can be used as retention gaps, guard columns or transfer lines. Our standard deactivation process is a phenyl methyl deactivation the preferred choice for most applications due to its inertness and robustness.

Fused Silica, Deactivated


ID (mm) OD (mm) Length (m) Part No.

0.05 0.05 0.05 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.18 0.18 0.18 0.20 0.20 0.20 0.25 0.25 0.25 0.25 0.32 0.32 0.32 0.32 0.45 0.45 0.45 0.53 0.53 0.53 0.53

0.36 0.36 0.36 0.19 0.19 0.19 0.36 0.36 0.36 0.36 0.34 0.34 0.34 0.34 0.34 0.34 0.35 0.35 0.35 0.35 0.43 0.43 0.43 0.43 0.67 0.67 0.67 0.67 0.67 0.67 0.67

1 5 10 1 5 10 1 5 5 10 1 5 10 1 5 10 1 5 10 30 1 5 10 30 1 5 10 1 5 10 30

160-2655-1 160-2655-5 160-2655-10 160-1010-1 160-1010-5 160-1010-10 160-2635-1 160-2635-5 19091-60620E* 160-2635-10

GC and GC/MS

160-2615-1 160-2615-5 160-2615-10 160-2205-1 160-2205-5 160-2205-10 160-2255-1 160-2255-5 160-2255-10 160-2255-30** 160-2325-1 160-2325-5 160-2325-10 160-2325-30** 160-2455-1 160-2455-5 160-2455-10 160-2535-1 160-2535-5 160-2535-10 160-2535-30**
*Wound on a 5 in. cage **Wound on a 7 in. cage

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311

GC Columns
Fused Silica Tubing
Fused Silica, High Temp (400C) Deactivated
ID (mm) OD (mm) Length (m) Part No.

0.05 0.10 0.18 0.25 0.25 0.32 0.32 0.53 0.53

0.36 0.36 0.34 0.35 0.35 0.43 0.43 0.67 0.67

5 5 5 5 10 5 10 5 10

160-2815-5 160-2825-5 160-2835-5 160-2845-5 160-2845-10 160-2855-5 160-2855-10 160-2865-5 160-2865-10

ProSteel Stainless Steel, Deactivated


ID (mm) OD (mm) Length (m) Part No.

0.53

0.67

160-4535-5

GC and GC/MS

312

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GC Columns
Fused Silica Tubing Undeactivated Tubing
Undeactivated tubing or bare fused silica is commonly used for capillary electrophoresis. It can also be used for transfer lines and other applications where inertness is not critical.

Fused Silica, Undeactivated


ID (mm) OD (mm) Length (m) Part No.

0.02 0.02 0.05 0.05 0.075 0.10 0.10 0.18 0.18 0.20 0.20 0.20 0.25 0.25 0.32 0.32 0.32 0.45 0.45 0.53 0.53

0.36 0.36 0.36 0.36 0.36 0.36 0.36 0.34 0.34 0.34 0.34 0.34 0.35 0.35 0.43 0.43 0.43 0.67 0.67 0.67 0.67

5 10 5 10 10 5 10 5 10 5 10 50 5 10 5 10 50 5 10 5 10

160-2660-5 160-2660-10 160-2650-5 160-2650-10 160-2644-10 160-2634-5 160-2634-10 160-2610-5 160-2610-10 160-2200-5 160-2200-10 19091-20050*

GC and GC/MS

160-2250-5 160-2250-10 160-2320-5 160-2320-10 19091-21050* 160-2450-5 160-2450-10 160-2530-5 160-2530-10


*Wound on a 7 in. cage

Stainless Steel, Undeactivated


ID (mm) OD (mm) Length (ft) Part No.

0.76 (1/32 in.)

1.59 (1/16 in.)

50

700-0013-50

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313

GC Columns
Column Installation and Tools

Column Installation Kit


The Capillary Column Installation Kit contains: Diamond-tipped pencil 20x magnifier Ferrule removal tool Septum removal tool Refillable 2 oz. bottle of Snoop
Column Installation Kit P/N 430-2000

Column Installation Kit


Description Column Installation Kit with Diamond Tip Pencil Part No. 430-2000

Column Rinse Kit


This kit makes solvent rinsing easy! Solvent rinsing removes many contaminants that accumulate in columns. The contaminants are removed by passing solvents through the column. Only bonded and cross-linked phase columns should be rinsed, or permanent damage to the column will result.

Column Rinse Kit


Description Capillary Rinse Kit Unit 10/pk Part No. 430-3000 0100-1375

GC and GC/MS

Column Rinse Kit P/N 430-3000

Replacement 1/16 in. Teflon Ferrule

Ceramic Wafers
For a full listing of cutting tools see page 88.

20x Magnifier
Examine column ends for proper cuts. Jagged, rough, or non-perpendicular cuts negatively affect chromatographic performance.

Column Ferrule Installation Tool


Tools
Column Ferrule Installation Tool P/N 19251-80680

Description Ceramic Wafer 20x Magnifier Column Ferrule Installation Tool

Unit 4/pk

Part No. 5181-8836 430-1020 19251-80680

314

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GC Columns
Stationary Phase Applications Guide

Method/Column Selection Guides


GC Columns Stationary Phase Applications Guide
GC Columns Stationary Phase Applications Guide Approximate Temperature Range (C) Phases With (Isothermal/Programmed)*Similar Selectivity

Agilent Phase

Composition

Polarity

General Applications

HP-1ms, DB-1ms, Amines, hydrocarbons, HP-1, DB-1 pesticides, PCBs, phenols, sulfur compounds, flavors and fragrances HP-5ms, DB-5, HP-5

100% Dimethylpolysiloxane

Non-polar

From -60 to 325/350

BP-1, SPB-1, CP-Sil 5, Rtx-1, OV-1, SE-30, 007-1, ZB-1 SPB-5, XTI-5, Mtx-5, CP-Sil 8CB, SE-54, Rtx-5, BPX-5, MDN-5, Rtx-5ms, BP-5, ZB-5

Semivolatiles, alkaloids, 5% Phenyl 95% drugs, FAMEs, halogenated dimethylpolysiloxane compounds, pesticides, herbicides

Non-polar

From -60 to 325/350

DB-5ms

Semivolatiles, alkaloids, 5% Phenyl drugs, FAMEs, halogenated 95% dimethyl compounds, pesticides, arylene siloxane herbicides Aroclors, alcohols, pesticides, VOCs CLP-pesticides, aroclors, pharmaceuticals, drugs of abuse CLP-pesticides, aroclors, pharmaceuticals, drugs of abuse Pesticides, herbicides, TMS sugars, aroclors Drugs, glycols, pesticides, steroids Drugs, glycols, pesticides, steroids 6% Cyanopropyl-phenyl Mid-polar 94% dimethyl polysiloxane 35% Phenyl 65% dimethyl Mid-polar polysiloxane 35% Phenyl 65% dimethyl arylene siloxane 14% Cyanopropyl-phenyl Mid-polar 86% dimethyl polysiloxane

From -60 to 325/350

SPB-5, XTI-5, Mtx-5, CP-Sil 8CB, SE-54, Rtx-5, BPX-5, MDN-5, Rtx-5ms Rtx-1301, Mtx-1301, CP-130 1 Rtx-35, SPB-35, AT-35, Sup-Herb, MDN-35, BPX-35 Rtx-35, SPB-35, AT-35, Sup-Herb, MDN-35, BPX-35 SPB-1701, CP-Sil 19 CB, Rtx-1701, CB-1701, OV-1701, 007-1701, BPX-10 Rtx-50, CP-Sil 19 CB, BPX-50, SP-2250

DB-1301 DB-35, HP-35

From -20 to 280/300 From 40 to 300/320

DB-35ms

From 50 to 340/360

GC and GC/MS

DB-1701, DB-1701P HP-50+, DB-17 DB-17ms

From -20 to 280/300

DB-200 DB-210 DB-225ms, DB-225 HP-INNOWax

50% Phenyl Mid-polar 50% dimethylpolysiloxane 50% Phenyl 50% dimethyl arylene siloxane Residual solvents, pesticides, 35% Trifluoropropyl Polar herbicides 65% dimethyl polysiloxane 50% Trifluoropropyl 50% dimethyl polysiloxane FAMEs, alditol acetates, 50% Cyanopropyl-phenyl Polar neutral sterols 50% dimethyl polysiloxane Polar

From 40 to 280/300 From 40 to 320/340

From 30 to 300/320 From 45 to 240/260 From 40 to 220/240

Rtx-200

Alcohols, free organic acids, Polyethylene glycol solvents, essential oils, flavors and fragrances DB-WAX Solvents, glycols, alcohols Polyethylene glycol CAM Amines, basic compounds Polyethylene glycol-base modified HP-FFAP, DB-FFAP Organic acids, alcohols, Polyethylene glycolaldehydes, ketones, acid modified acrylates DB-23 FAMEs (requiring 50% Cyanopropyl cis/trans resolution) 50% dimethyl polysiloxane CycloSil-B Chiral compounds 30%-heptakis (general purpose) (2,3-di-O-methyl-6-O-t-butyl dimethylsilyl)-B-cyclodextrin in DB-1701 HP-Chiral Chiral compounds (using a beta-Cyclodextrin in phenylColumns Nitrogen selective detector, based stationary phase NPD)

From 40 to 260/270

Polar Polar Polar

From 20 to 250/260 From 60 to 220/240 From 40 to 250

Polar Mid-polar

From 40 to 250/260 From 35 to 260/280

SP-2330, CP-Sil 43 CB, OV-225, Rtx-225, BP-225, 007-225 BP-20, 007-CW, CP-WAX 52 CB, Stabilwax, Supelcowax-10 Rt-Wax Carbowax Amine, Stabilwax-DB, CP-51 WAX OV-351, SP-1000, Stabilwax-DA, 007-FFAP, Nukol Rtx-2330, 007-23, SP-2330/2340/2380/2560 LIPODEX C, Rt-BDEXm, B-DEX 110, B-DEX 120

Mid-polar

From 30 to 240/250

LIPODEX C, Rt-BDEXm, B-DEX 110, B-DEX 122

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315

GC Columns
Stationary Phase Applications Guide
GC Columns Stationary Phase Applications Guide (continued)
Approximate Temperature Range (C) Phases With (Isothermal/Programmed)*Similar Selectivity

Agilent Phase

Composition

Polarity

PLOT Column Applications

GC and GC/MS

HP-PLOT Molesieve Permanent and noble gases. Argon and oxygen separation at 35C HP-PLOT C1-C6 hydrocarbons in Al2O3 KCl natural gas, refinery gas, fuel gas, synthetic gas, dienes HP-PLOT C1-C6 hydrocarbons in Al2O3 S natural gas, refinery gas, fuel gas, synthetic gas, dienes GS-Alumina C1-C6 hydrocarbons in natural gas, refinery gas, fuel gas, synthetic gas, dienes HP-PLOT Q Hydrocarbons including isomers, CO2, methane, air/CO, water, polar solvents, sulfur compounds HP-PLOT U C1 to C7 hydrocarbons, CO2, methane, air/CO, water, oxygenates, amines, solvents, alcohols, ketones, aldehydes GS-GasPro C1 to C12 hydrocarbons, CO2, trace-level sulfurs, hydride gases, inorganic gases, halocarbons, SF6, oxygen/nitrogen separation at 80C GS-CarbonPLOT C1 to C5 hydrocarbons, CO2, air/CO, trace acetylene in ethylene, methane
Specialty Phases - Environmental Applications

5 molecular sieve zeolite

From -60 to 300

Rt-Molesieve 13X, Molesieve 5 AluminaPlot, Rt-Alumina, CP-Al2O3/KCl Plot

Aluminum Oxide "KCl" deactivated Aluminum Oxide "Sodium Sulfate" deactivated Aluminum Oxide with proprietary deactivation Polystyrene-divinylbenzene

Least polar

From -60 to 200

Mid-polar

From -60 to 200

Most polar

From -60 to 200

From -60 to 270/290

PoraPlot Q/S, Rt-Q, Supel-Q PLOT

Divinylbenzene/ethylene glycol dimethacrylate

From -60 to 190

PoraPlot U

Proprietary, bonded silica-based

From -80 to 260/300

CP-SilicaPLOT

Bonded monolithic carbon layer

From 0 to 360

CP-CarboBond

DB-624 DB-VRX DB-35ms

DB-XLB (Confirmation Column) HP-5ms

6% Cyanopropyl-phenyl Mid-polar 94% dimethyl polysiloxane Volatile Organic Compounds Proprietary phase Non-polar using MSD, ELCD/PID CLP Pesticides, Chlorinated 35% Phenyl Mid-polar Herbicides, PCBs, 65% dimethyl 508.1 Pesticides arylene siloxane Proprietary phase Non-polar

From -20 to 260C From -10 to 260C From 50 to 340/360C

Rtx-624, AT-624, SPB-624, CP-624, PE-624,007-624 Rtx-VRX MDN-35, BPX-35

From 30 to 340/360C

No equivalent

Semivolatiles by EPA Method 8270 PCB Congener Analysis (209 Congeners) CLP Pesticides, Chlorinated Herbicides, PCBs, 508.1 Pesticides Leaking Underground Fuel Tank (LUFT) testing MTBE in Soil and Water

5% Phenyl 95% dimethylpolysiloxane Proprietary phase

Non-polar

DB-XLB

Non-polar

From -60 to 325/350C SPB-5, XTI-5, Mtx-5, CP-Sil 8CB, SE-54, Rtx-5, BPX-5, MDN-5, Rtx-5ms From 30 to 340/360C

DB-TPH DB-MTBE

Proprietary phase Proprietary phase

Non-polar Non-polar

From -10 to 290C From 35 to 260/280 From -20 to 260C

Other Specialty Phases

HP-Fast GC Residual Solvents Residual Solvent Column DB-ALC1 Blood Alcohol Testing DB-ALC2 HP-Blood Alcohol Column

6% Cyanopropyl-phenyl Mid-polar 94% dimethyl polysiloxane Proprietary phase Proprietary phase Proprietary phase Mid-polar Mid-polar

From 20 to 260/280C From 20 to 260/280C From -60 to 270/290

*Temperature limits vary with column dimensions. Always confirm temperature limits prior to column use.

316

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GC Columns
Environmental/EPA Methods Environmental/EPA Methods
Many possible column and instrument combinations can be used to obtain successful Environmental and EPA Analyses. Listed below are a few of the columns Agilent would recommend for these analyses. The following recommendations are based upon GCs equipped with split/splitless injectors (except for the volatiles methods). Other column configurations may be suitable with different instrument configurations. To tailor your analytical system to your particular needs, contact your local Aglilent office for the best column recommendation.
Environmental/EPA Methods
Analyte Type
Volatiles

EPA Method Common Sample Reference Preparation 501 Purge and trap, direct injection, headspace Purge and trap, direct injection, headspace

Detector Types ELCD, ECD

Sample Matrix Drinking water

Agilent Column Recommendation (Part No.) DB-VRX, 30 m x 0.45 mm x 2.55 m (124-1534) DB-624, 30 m x 0.45 mm x 2.55 m (124-1334) DB-VRX, 75 m x 0.45 mm x 2.55 m (124-1574) DB-624, 75 m x 0.45 mm x 2.55 m (124-1374) DB-VRX, 75 m x 0.45 mm x 2.55 m (124-1574) DB-624, 75 m x 0.45 mm x 2.55 m (124-1374) DB-VRX, 30 m x 0.45 mm x 2.55 m (124-1534) DB-624, 30 m x 0.45 mm x 2.55 m (124-1334) DB-VRX, 60 m x 0.25 mm x 1.4 m (122-1564) DB-624, 60 m x 0.25 mm x 1.4 m (122-1364) HP-VOC, 60 m x 0.20 mm x 1.1 m (19091R-306) DB-VRX, 20 m x 0.18 mm x 1.0 m (121-1524) DB-624, 20 m x 0.18 mm x 1.0 m (121-1324) DB-VRX, 30 m x 0.45 mm x 2.55 m (124-1534) DB-624, 30 m x 0.45 mm x 2.55 m (124-1334) DB-VRX, 30 m x 0.45 mm x 2.55 m (124-1534) DB-624, 30 m x 0.45 mm x 2.55 m (124-1334) HP-5ms, 30 m x 0.25 mm x 0.5 m (19091S-133)

Trihalomethanes

Volatile Organic Compounds (VOCs)

502.2, 8021, CLP-Volatiles

PID, ELCD

Purgeable 601, 8010 Halogenated Organics Purgeable Aromatic Organics Volatile Organic Compounds (VOCs) Using MSD Volatile Organic Compounds (VOCs) Using 5973 MSD EDB and DBCP Acrylonitrile and Acrolein
Semivolatiles

Purge and trap, PID, ELCD headspace for screening Purge and headspace for screening Purge and trap, direct injection, headspace Purge and trap, direct injection, headspace Microextraction with Hexane Purge and trap, liquid extraction, sonication Liquid extraction, sonication, soxhlet extraction, SPE Liquid extraction, sonication, soxhlet extraction, derivatization Liquid extraction, sonication, soxhlet extraction, SPE Liquid extraction PID

Drinking water, waste water, solid wastes Waste water, solid wastes

503.1, 602, 8020 524.2, 624, 8240, 8260, CLP-VOCs 524.2, 624, 8240, 8260, CLP-VOCs 504.1, 8011 603, 8015, 8031 525, 625, 8270 528, 604, 8040, 8041

Drinking water, trap, waste water, solid wastes MSD Drinking water, waste water, solid wastes MSD (5973) Drinking water, waste water, solid wastes ECD Drinking water, solid wastes FID, NPD Waste water, solid wastes MSD Drinking water, waste water, solid wastes Waste water, solid wastes

GC and GC/MS

Semivolatile Organic Compounds Phenols

ECD, FID

Phthalate Esters

506, 606, 8060, 8061 605

ECD, FID

Benzidines

ECD

Drinking water, waste water, solid wastes Waste water

Nitrosamines

607, 8070

Nitroaromatics and Isophorone Polynuclear Aromatic Hydrocarbons (PAHs) Chlorinated Hydrocarbons

609, 8090

610, 8100

612, 8120, 8121

Chlorinated Disinfection 551, 551.1A Byproducts Halogenated 552, 552.1, Acetic Acids 552.2

Liquid extraction, sonication, soxhlet extraction, SPE Liquid extraction, sonication, soxhlet extraction, SPE Liquid extraction, sonication, soxhlet extraction, SPE Liquid extraction, sonication, soxhlet extraction, SPE Liquid extraction, derivatization Liquid extraction, derivatization

NPD

Waste water, solid wastes Waste water, solid wastes Waste water, solid wastes Waste water, solid wastes Drinking water Drinking water

DB-5ms, 30 m x 0.25 mm x 0.25 m (122-5532) DB-XLB, 30 m x 0.25 mm x 0.25 m (122-1232) DB-5ms, 30 m x 0.53 mm x 1.5 m (125-5532) DB-608, 30 m x 0.53 mm x 0.5 m (125-6837) DB-5ms, 30 m x 0.25 mm x 0.25 m (122-5532) DB-5ms, 30 m x 0.53 mm x 1.5 m (125-5532) DB-608, 30 m x 0.53 mm x 0.5 m (125-6837) DB-5ms, 30 m x 0.25 mm x 0.25 m (122-5532) DB-5ms, 30 m x 0.53 mm x 1.5 m (125-5532) DB-608, 30 m x 0.53 mm x 0.5 m (125-6837) DB-5ms, 30 m x 0.25 mm x 0.25 m (122-5532) DB-5ms, 30 m x 0.53 mm x 1.5 m (125-5532) HP-5ms, 30 m x 0.25 mm x 0.5 m (19091S-133) DB-5ms, 30 m x 0.53 mm x 1.5 m (125-5532) DB-608, 30 m x 0.53 mm x 0.5 m (125-6837) DB-5ms, 30 m x 0.25 mm x 0.25 m (122-5532) DB-5ms, 30 m x 0.32 mm x 0.25 m (123-5532) DB-1ms, 30 m x 0.25 mm x 0.25 m (122-0132) DB-5ms, 30 m x 0.32 mm x 0.5 m (123-5536) HP-5ms, 30 m x 0.32 mm x 0.5 m (19091S-113) DB-1, 30 m x 0.32 mm x 0.5 m (123-103E) DB-5ms, 30 m x 0.25 mm x 1.0 m (122-5533) DB-1, 30 m x 0.25 mm x 1.0 m (122-1033) DB-35ms, 30 m x 0.32 mm x 0.25 m (123-3832) DB-XLB, 30 m x 0.32 mm x 0.5 m (123-1236)

ECD, FID

FID

ECD

ECD ECD

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317

GC Columns
ASTM Method Guide
Environmental/EPA Methods (continued)
Analyte Type Organochlorine Pesticides and PCBs Phenoxy Acid Herbicides N- and P-Containing Pesticides and Herbicides PCB Congeners Using MSD EPA Method Common Sample Reference Preparation 508.1, 608, 8081A, 8082, CLP-Pesticides 515, 615, 8150, 8151 507, 614, 619, 622, 8140, 8141A Liquid extraction, sonication, soxhlet extraction, SPE Liquid extraction, sonication, soxhlet extraction, SPE Liquid extraction, sonication, soxhlet extraction, SPE Detector Types ECD Sample Matrix Agilent Column Recommendation (Part No.) DB-35ms, 30 m x 0.32 mm x 0.25 m (123-3832) DB-XLB, 30 m x 0.32 mm x 0.5 m (123-1236) DB-35ms, 30 m x 0.32 mm x 0.25 m (123-3832) DB-XLB, 30 m x 0.32 mm x 0.5 m (123-1236) DB-35ms, 30 m x 0.25 mm x 0.25 m (122-3832) DB-5ms, 30 m x 0.25 mm x 0.25 m (122-5532) DB-XLB, 30 m x 0.18 mm x 0.18 m (121-1232) DB-XLB, 60 m x 0.25 mm x 0.25 m (122-1262)

Pesticides, Herbicides and PCBs

Drinking water, waste water, solid wastes ECD Drinking water, waste water, solid wastes NPD, ELCD, Drinking water, FPD waste water, solid wastes MSD

ASTM
ASTM Methods
Method Designation D 1945 Method Title Standard Test Method for the Analysis of Natural Gas by GC Standard Test Method for the Analysis of Reformed Gas by GC Standard Test Method for Fatty Acid Composition by Gas-Liquid Chromatography of Methyl Esters Standard Test Method for the Analysis of Liquified Petroleum (LP) Gases and Propene Concentrates by GC Column Recommendation HP-Molesieve, 15 m x 0.53 mm id, 50 m HP-PLOT Q, 15 m x 0.53 mm id, 40 m D 1946 HP-Molesieve, 15 m x 0.53 mm id, 50 m HP-PLOT Q, 15 m x 0.53 mm id, 40 m D 1983 DB-WAX, 30 m x 0.25 mm id, 0.25 m film Part No. 19095P-MS9 19095P-QO3 19095P-MS9 19095P-QO3 122-7032

GC and GC/MS

D 2163

HP-Alumina/KCl, 30 m x 0.53 mm id (Agilent preferred) HP-Alumina S, 30 m x 0.53 mm id (Agilent preferred alternate)

19095P-K23

19095P-S23

D 2268 122-106E D 2306 D 2426

Standard Test Method for Analysis of High-Purity n-Heptane and Isooctane by Capillary GC Standard Test Method fo C8 Aromatic Hydrocarbons by GC Standard Test Method for Butadiene Dimer and Styrene in Butadiene Concentrates by GC Standard test Method for Determination of C2 through C5 Hydrocarbons in Gasoline by GC Standard Test Method for Noncondensable Gases in C2 and Lighter Hydrocarbon Products by GC Standard Test Method for Ethylene, Other Hydrocarbons, and Carbon Dioxide in High-Purity Ethylene by GC Standard Test Method for Butadiene Purity and Hydrocarbon Impurities by GC Standard Test Method for Hydrocarbon Traces in Propylene Concentrates by GC Standard Test Method for Purity of Methyl Ethyl Ketone by GC Standard Test Method for Boiling Range Distribution of Petroleum Fractions by GC Standard Test Method for Boiling Range Distribution of Petroleum Fractions by GC, to C60

DB-1, 60 m x 0.25 mm id, 0.50 m film (Agilent preferred) HP-INNOWax, 60 m x 0.25 mm id, 0.25 m film (Agilent preferred) DB-1, 30 m x 0.53 mm id, 5.00 m film (Agilent preferred) DB-1, 30 m x 0.53 mm id, 5.0 m film (Agilent preferred) GS- Alumina, 30 m x 0.53 mm id (Agilent preferred) 19091N-136 125-1035

D 2427

125-1035

115-3532 19095P-MS0

D 2504

HP-Molesieve, 30 m x 0.53 mm id, 50 m film

D 2505

GS-GasPro, 60 m x 0.32 mm id (Agilent preferred)

113-4362

D 2593 D 2712 D 2804

GC-Alumina, 30 m x 0.53 mm id (Agilent preferred) GS-Alumina, 50 m x 0.53 mm id (Agilent preferred) DB-WAX, 30 m x 0.53 mm id, 1.0 m film (Agilent preferred) DB-210, 15 m x 0.53 mm id, 1.0 m film DB-2887, 10 m x 0.53 mm id, 3.00 m film

115-3532 115-3552 125-7032 125-0212 125-2814

D 2887

"Extended D 2887"

HP-1, 10 m x 0.53 mm id, 0.88 m film

19095Z-021

HP-1, 5 m x 0.53 mm id, 0.88 m film

19095Z-020

318

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GC Columns
ASTM Method Guide
ASTM Methods (continued)
Method Designation D 2908 Method Title Standard Practice for Measuring Volatile Organic Matter in Water by Aqueous-Injection GC Standard Test Method for Analysis of Cyclohexane by GC Standard Test Method for Aromatics in Mineral Spirits by GC Standard Test Method for Purity of Methyl Isobutyl Ketone by GC Column Recommendation Contact Agilent for recommendation Part No.

D 3054 D 3257 D 3329

DB-1, 60 m x 0.32 mm id, 0.50 m film DB-624, 30 m x 0.53 mm id, 3.0 m film (Agilent preferred) DB-WAX, 30 m x 0.53 mm id, 1.0 m film (Agilent preferred) DB-624, 30 m x 0.45 mm id, 2.55 m film (Agilent preferred)

123-106E 125-1334 125-7032 124-1334 19091S-713

D 3432

Standard Test Method for Unreacted Toluene Diisocyanates in Urethane Prepolymers and Coating Solutions by GC Standard Test Method for Purity of Halogentated Organic Solvents

HP-1ms, 30 m x 0.32 mm id, 1.00 m film

D 3432 D 3447 D 3534 D 3545 D 3687

DB-1, 30 m x 0.45 mm id, 0.42 m film DB-624, 30 m x 0.53 mm id, 3.0 m film (Agilent preferred)

124-1037 125-1334 124-1232 125-1334 125-7032

Standard Test Method for PCBs in Water DB-XLB, 30 m x 0.45 mm id, 1.27 m film (Agilent preferred) Standard Test Method for Alcohol DB-624, 30 m x 0.45 mm id, 2.55 m film Content and Purity of Acetate Esters by GC (Agilent preferred) Standard Practice for Analysis of Organic DB-WAX, 30 m x 0.53 mm id, 1.0 m film Vapors Collected by the Activated Charcoal Tube Adsorption Method DB-WAX, 30 m x 0.45 mm id, 0.85 m film Standard Test Method for Volatile Alcohols in Water by Direct AqueousInjection GC DB-WAX, 30 m x 0.53 mm id, 1.0 m film (Agilent preferred)

124-7032 125-7032

D 3695

D 3710

Standard Test Method for Boiling Range DB-2887, 10 m x 0.53 mm id, 3.00 m film Distribution of Gasoline and Gasoline Fractions by GC Standard Test Method for Analysis of Isopropylbenzene (Cumene) by GC Standard Test Method for Analysis of o-Xylene by GC Standard Test Method for Analysis of p-Xylene by GC Standard Test Method for Purgeable Organic Coumpounds in Water Using Headspace Sampling Standard Test Method for Purity of Methyl Amyl Ketone and Methyl Isoamyl Ketone by GC

125-2814

GC and GC/MS

D 3760

DB-WAX 60 m x 0.32 mm id, 0.25 m film (Column A) 123-7062 HP-1, 50 m x 0.32 mm id, 0.52 m film (Column B) 19091Z-115 19091N-216 19091N-216 124-1574

D 3797 D 3798 D 3871

HP-INNOWax, 60 m x 0.32 mm id, 0.50 m film HP-INNOWax, 60 m x 0.32 mm id, 0.50 m film DB-VRX, 75 m x 0.45 mm id, 2.55 m film (Agilent preferred) DB-624, 30 m x 0.53 mm id, 3.0 m film (Agilent preferred)

D 3893

125-1334

D 3973 D 4415 D 4424 D 4443

Standard Test Method for Low-Molecular DB-VRX, 30 m x 0.45 mm id, 2.55 m film Weight Halogenated Hydrocarbons in Water (Agilent preferred) Standard Test Method for Determination DB-FFAP, 30 m x 0.32 mm id, 0.25 m film of Dimer in Acrylic Acid Standard Test Method for Butylene Analysis by GC HP-AluminaS, 50 m x 0.53 mm id (Agilent preferred)

124-1534 123-3232 19095P-S25 124-1534

Standard Test Method for Residual Vinyl DB-VRX, 30 m x 0.45 mm id, 2.55 m film Chloride Monomer Content in PPB Range (Agilent preferred) in Vinyl Chloride Homo- and Co-Polymers by Headspace GC Standard Test Method for Determination of DB-FFAP, 30 m x 0.45 mm id, 0.85 m film Trace Thiophene in Refined Benzene by GC Standard Test Method for Propylene DB-5, 10 m x 0.32 mm id, 5.0 m film Gycol Monomethyl Ether, Dipropylene Glycol Monomethyl Ether, and Propylene Glycol Monmethyl Ether Acetate DB-5, 30 m x 0.45 mm id, 4.25 m film (Agilent preferred)

D 4735

124-3232

D 4773

100-2000

124-5035 124-7032

D 4864

Standard Test Method for Determination DB-WAX, 30 m x 0.45 mm id, 0.85 m film of Traces of Methanol in Propylene Concentrates by GC

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319

GC Columns
ASTM Method Guide
ASTM Methods (continued)
Method Designation D 4947 Method Title Standard Test Method for Chlordane and Heptachlor Residues in Indoor Air Column Recommendation DB-5, 30 m x 0.53 mm id, 1.5 m film DB-608, 30 m x 0.53 mm id, 0.83 m film (confirmation column, Agilent preferred) D 4961 Standard Test Method for GC Analysis of Major Organic Impurities in Phenol Produced by the Cumene Process Standard Test Method for Cyclohexylamine, Morpholine and Diethylaminoethanol in Water and Condensed Steam by Direct Aqueous Injection GC Part No. 125-5032 125-1730

DB-FFAP, 30 m x 0.45 mm id, 0.85 m film (Method A) 124-3232

HP-PLOT Q 15 m x 0.53 mm id (Method B) D 4983 HP-5ms 30 m x 0.32 mm id, 1.00 m film (Agilent preferred)

19095P-QO3 19091S-213

CAM, 30 m x 0.53 mm id, 1.00 m film (alternate column) D 5008 Standard Test Method for Ethyl Methyl Pentonal Content and Purity Value of 2-Ethylhexanol by GC HP-1, 15 m x 0.53 mm id, 5.0 m film

115-2132 19095Z-621

HP-INNOWax, 30 m x 0.32 mm id, 0.25 m film D 5060 D 5075 Standard Test Method for Determining HP-INNOWax, 60 m x 0.32 mm id, 0.50 m film Impurities in High-Purity Ethylbenzene by GC Standard Test Method for Nicotine in Indoor Air Standard Test Method for Detailed Analysis of Petroleum Naphthas Through n-Nonane by Capillary GC Standard Test Method for Analysis of Styrene by Capillary GC DB-5, 30 m x 0.53 mm id, 1.5 m film DB-5, 30 m x 0.32 mm id, 1.0 m film D 5134 HP-PONA, 50 m x 0.20 mm id, 0.50 m film

19091N-113 19091N-216 125-5032 123-5033 19091S-001

D 5135

HP-INNOWax, 60 m x 0.32 mm id, 0.50 m film

19091N-216 123-1033

GC and GC/MS

D 5175

Standard Test Method for Organohalide DB-1, 30 m x 0.32 mm id, 1.0 m film (Column 1) Pesticides and Polychlorinated Biphenyls in Water by Microextraction and GC DB-608, 30 m x 0.32 mm id, 0.5 m film (Column 3) DX-3, 30 m x 0.32 mm id, 0.25 m film (Column 2) DB-XLB, 30 m x 0.25 mm id, 0.25 m film (Agilent preferred)

123-1730 123-6332 122-1232 113-4332 19095P-QO4 19095Z-627

D 5303

Standard Test Method for Trace Carbonyl GS-GasPro, 30 m x 0.32 mm id (Agilent preferred) Sulfide in Propylene by GC HP-PLOT Q 30 m x 0.53 mm id, 40 m Standard Test Method for Determination HP-1, 7.5 m x 0.53 mm id, 5.0 m film of Boiling Range Distribution of Crude (Agilent preferred) Petroleum by GC Standard Test Method for Tar Acid Composition by Capillary GC HP-5ms, 30 m x 0.25 mm id, 0.25 m film DB-225ms, 30 m x 0.25 mm id, 0.25 m film (recommended)

D 5307

D 5310

19091S-433 122-2932 19091S-713

D 5316

Standard Test Method for 1, 2-Dibromoethane and 1, 2-Dibromo-3-Chloropropane in Water by Microextraction and GC

HP-1ms, 30 m x 0.32 mm id,1.00 m film (Column B)

DB-624, 30 m x 0.45 mm id, 2.55 m film (Agilent preferred Column B alternative) DX-3, 30 m x 0.32 mm id, 0.25 m film (Column A) D 5317 Standard Test Method for HP-5ms 30 m x 0.25 mm id, 0.25 m film (Column 1) Determination of Chlorinated Organic Acid Coumpounds in Water by GC with Electron Capture Detector DB-XLB, 30 m x 0.25 mm id, 0.25 m film (Agilent preferred) DB-35ms 30 m x 0.25 mm id, 0.25 m film (Agilent preferred) D 5320 Standard Test Method for Determination DB-1 30 m x 0.53 mm id, 3.0 m film of 1,1-Trichloroethane and Methylene Chloride in Stabilized Trichloroethylene and Tetrachloroethylene DB-VRX, 30 m x 0.32 mm id, 1.80 m film (Agilent preferred)

124-1334 123-6332 19091S-433

DB-1701P, 30 m x 0.25 mm id, 0.25 m film (Column 2) 122-7732 122-1232 122-3832 125-1034

123-1534

320

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GC Columns
ASTM Method Guide
ASTM Methods (continued)
Method Designation D 5399 D 5441 Method Title Column Recommendation Standard Test Method fo Boiling Point DB-2887, 10 m x 0.53 mm id, 3.00 m film Distribution of Hydrocarbon Solvents by GC Standard Test Method for Analysis of Methyl Tert-Butyl Ether (MTBE) by GC Standard Test Method for Analysis of Petroleum Waxes by GC Standard Test Method for Nitrogenand Phosphorus-Containing Pesticides in Water by GC with a NitrogenPhosphorus Detector HP-PONA, 50 m x 0.20 mm id, 0.50 m film DB-Petro, 100 m x 0.25 mm id, 0.50 m film D 5442 DB-1, 25 m x 0.32 mm id, 0.25 m film DB-5, 15 m x 0.25 mm id, 0.25 m film D 5475 HP-5ms, 30 m x 0.25 mm id, 0.25 film (recommended in method) Part No. 125-2814 19091S-001 122-10A6 123-1022 122-5012 19091S-433

DB-1701P, 30 m x 0.25 mm id, 0.25 m film (recommended in method) DB-XLB, 30 m x 0.25 mm id, 0.25 m film (Agilent preferred) DB-35ms 30 m x 0.25 mm id, 0.25 m film (Agilent preferred) D 5480 D 5501 Standard Test Method for Engine Oil Volatility by GC DB-PS1 15 m x 0.53 mm id, 0.15 m film (Agilent preferred)

122-7732 122-1232 122-3832 145-1011 19091Z-530

Standard Test Method for Determination HP-1, 100 m x 0.25 mm id, 0.50 m film of Ethanol Content of Denatured Fuel Ethanol by GC Standard Test Method for Determination HP PLOT Q, 15 m x 0.53 mm id of Trace Organic Impurities in Monomer Grade Vinyl Chloride by Capillary Column/Multi-dimensional GC HP PLOT U, 30 m x 0.53 mm id Standard Test Method for Determination HP PLOT Q, 30 m x 0.53 mm id of Residual Acrylonitirle Monomer in Styrene-Acrylonitirle Co-poplymer Resins and Nitrile-Butadiene Rubber by Headspace-Capillary GC Standard Test Method for Determination DB-1, 30 m x 0.53 mm id, 5.0 m film of Benzene, Toluene, Ethylbenzene, (capillary column) p/m-Xylene, C9 and Heavier Aromatics, and Total Aromatics in Finished Gasoline by GC Standard Test Method for Determination HP-1, 60 m x 0.25 mm id, 1.0 m film of Oxygenates in Gasoline by GC and Oxygen Selective Flame Ionization Detection Standard Test Method for Sulfur Compounds in Light Petroleum Liquids by GC and Sulfur Selective Detection HP-1, 30 m x 0.32 mm id, 4.0 m film

D 5507

19095P-QO3

19095P-UO4 19095P-QO4

D 5508

GC and GC/MS

D 5580

125-1035

D 5599

19091Z-236

D 5623

19091Z-613

D 5713

Standard Test Method for Analysis of High DB-PETRO, 50 m x 0.20 mm id, 0.50 m film Purity Benzene for Cyclohexane Feedstock by Capillary GC Standard Practice for Oil Spill Source Identification by GC and Positive Ion Electron Impact Low Resolution Mass Spectrometry DB-5, 30 x 0.25 mm id, 0.25 m film

128-1056

D 5739

122-5032

DB-TPH, 30 m x 0.25 mm id, 0.25 m film D 5769 Standard Test Method for Determination HP-1, 60 m x 0.25 mm id, 1.0 m film of Benzene, Toluene, and Total Aromatics in Finished Gasoline by GC/MS Standard Test Method for Measurement DB-VRX, 60 m x 0.25 mm id, 1.40 m film of Purgeable Organic Compunds in Water (for 5972 and older MSDs) by Capillary Column GC/MS DB-VRX, 20 m x 0.18 mm id, 1.0 m film (for 5973 MSD) DB-624, 60 m x 0.25 mm id, 1.40 m film (for 5972 and older MSDs) DB-624, 20 m x 0.18 mm id, 1.0 m film (for 5973 MSD)

123-1632 19091Z-236

D 5790

122-1564

121-1524 122-1364 121-1324

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GC Columns
ASTM Method Guide
ASTM Methods (continued)
Method Designation D 5812 Method Title Column Recommendation Standard Test Method for Determination HP-5ms, 30 m x 0.25 mm id, 0.25 m film of Organochlotine Pesticides in Water (recommended in method) by Capillary Column GC DB-1701P, 30 m x 0.25 mm id, 0.25 m film (recommended in method) DB-XLB, 30 m x 0.25 mm id, 0.25 m film (Agilent preferred) DB-35ms 30 m x 0.25 mm id, 0.25 m film (Agilent preferred) D 5917 Standard Test Method for Trace Impurities HP-INNOWax, 60 m x 0.32 mm id, 0.25 m film in Monocyclic Aromatic Hydrocarbons by GC and External Calibration Standard Test Methods for Fatty and Rosin Acids in Tall Oil Fraction Products by Capillary GC DB-23, 60 m x 0.25 mm id, 0.25 m film Part No. 19091S-433

122-7732 122-1232 122-3832 19091N-116

D 5974

122-2362

D 5986

Standard Tests Method for Determination HP-1, 60 m x 0.53 mm id, 5.0 m film of Oxygenates, Benzene, Toluene, C8-C12 Aromatics and Total Aromatics in Finished Gasoline by GC/FTIR Standard Test Method for Trace Impurities HP-1, 60 m x 0.25 mm id, 1.0 m film in Alpha-Methylstyrene by Capillary GC

19095Z-626

D 6144 D 6159

19091Z-236

Standard Test Method for Determination of HP-PLOT Alumina/KCl, 50 m x 0.53 mm id (Column 1) 19095P-K25 Hydrocarbon Impurities in Ethylene by GC GS-Alumina, 50 m x 0.53 mm id (Column 1, Agilent preferred) DB-1, 30 m x 0.53 mm id, 5.0 m film (Column 2) 115-3552 125-1035 19091S-413 122-1232 145-1001

D 6160

Standard Test Method for Determination HP-5ms, 30 m x 0.32 mm id, 0.25 m film of PCBs in Waste Materials by GC DB-XLB, 30 m x 0.25 mm id, 0.25 m film (Agilent preferred)

GC and GC/MS

D 6352

Standard Test Method for Boiling Range DB-HT Sim Dis, 5 m x 0.53 mm id, 0.15 m film Distribution of Petroleum Distillates in Boiling Range from 174 to 700 by GC Standard Test Method for Estimation of Engine Oil Volatility by Capillary GC DB-HT Sim Dis, 5 m x 0.53 mm id, 0.15 m film

D 6417 D2360 E 1616 E 1863 E 202 E 475

145-1001 19091N-116 19091Z-115 123-7364 125-1334 19095J-623

Standard Test Method for Trace Impurities HP-INNOWax, 60 m x 0.32 mm id, 0.25 m film in Monocylic Aromatic Hydrocarbons by GC Standard Test for Analysis of Acetic Anhydride Using GC Standard Test Method for Analysis of Acrylonitrile by GC Standard Test Methods for Analysis of Ethylene Glycols and Propylene Glycols Standard Test Method for Assay of Di-tert-Butyl Peroxide Using GC HP-1, 50 m x 0.32 mm id, 0.52 m film DB-WAXetr, 60 m x 0.32 mm id, 1.0 m film DB-624, 30 m x 0.53 mm id, 3.0 m film (Agilent preferred) HP-5, 30 m x 0.53 mm id, 5.0 m film

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GC Columns
USP GC Phases

USP GC Phases
United States Pharmacopoeia (USP GC Phases) USP G1 G2 G3 G5 G6 G7 G14 G15 G16 G17 G19 G20 G25 G27 G28 G32 G35 G36 G38 G39 G41 G42 G43 G45 G46 Phase Composition Dimethylpolysiloxane oil Dimethylpolysiloxane gum 50% Phenyl - 50% methylpolysiloxane 3-cyanopropyl polysiloxane Trifluoropropylmethylpolysilicone 50% 3-Cyanopropyl - 50% phenylmethylsilicone Polyethylene glycol (average molecular weight of 950-1,050) Polyethylene glycol (average molecular weight of 3,000-3,700) Polyethylene glycol (average molecular weight of 15,000) 75% Phenyl - 25% methylpolysiloxane 25% Phenyl - 25% cyanopropylmethylsilicone Polyethylene glycol (average molecular weight of 380-420) Polyethylene glycol TPA (Carbowax 20M terephthalic acid) 5% Phenyl - 95% methylpolysiloxane 25% Phenyl - 75% methylpolysiloxane 20% Phenylmethyl - 80% dimethylpolysiloxane 1% Vinyl - 5% phenylmethylpolysiloxane Phase G1 plus a tailing inhibitor Polyethylene glycol (average molecular weight of 1,500) Phenylmethyldimethylsilicone (10% phenyl substituted) 35% Phenyl - 65% dimethylvinylsiloxane 6% Cyanopropylphenyl - 94% dimethylpolysiloxane Divinylbenzene-ethylene glycol-dimethacrylate 14% Cyanopropylphenyl - 86% methylpolysiloxane Agilent Phase Recommendation HP-1*, DB-1*, HP-1ms*, DB-1ms* HP-1*, DB-1*, HP-1ms*, DB-1ms* DB-17*, HP-50+* DB-23 DB-200, DB-210 DB-225, DB-225ms DB-WAX DB-WAX DB-WAX* DB-17, HP-50+ DB-225*, DB-225ms DB-WAX DB-FFAP*, HP-FFAP* DB-5*, HP-5*, HP-5ms*, DB-5ms DB-35, HP-35, DB-35ms DB-35, HP-35, DB-35ms DB-5, HP-5, HP-5ms, DB-5ms DB-1, HP-1, HP-1ms, DB-1ms DB-WAX DB-5, HP-5, HP-5ms, DB-5ms DB-35*, HP-35*, DB-35ms DB-624*, DB-1301 HP-PLOT U* DB-1701* *Indicates an exact equivalent

GC and GC/MS

Polyethylene glycol & diepoxide esterified with nitroterepthalic acid DB-FFAP*, HP-FFAP*

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GC and GC/MS
Troubleshooting/Maintenance

Column Installation and Tools


Capillary Column Installation Quick Reference Guide
For more detailed installation information, refer to the GC Column Installation Guide which is provided with each column.

Precolumn Installation Check List


1. 2. Replace oxygen, moisture, and hydrocarbon traps as needed. Check gas cylinder pressures to ensure that an adequate supply of carrier, makeup, and fuel gases is available. Minimum recommended carrier gas purity percentages are: Helium 99.995 and Hydrogen 99.995. Clean the injection port, replace critical injection port seals, replace injection port liners, and change septa as needed. Check detector seals, and replace as necessary. Clean or replace detector jets as necessary. Carefully inspect the column for damage or breakage. Gather the necessary installation tools: You will need a column cutter, column nuts, ferrules, a magnifying loupe, and typewriter correction fluid.

3. 4. 5. 6.

Installing the Column


1. 2. 3.
0.4 0.4 0.4 0.4 0.5 0.8 0.8

GC and GC/MS

Table 1. Ferrule Sizes


Column ID 0.10 0.18 0.20 0.25 0.32 0.45 0.53 Ferrule ID (mm)

4. 5.

6. 7.

8.

Uncoil approximately 0.5 m of tubing from the column basket at both ends of the column for injector and detector installation. Avoid sharp bends in the tubing. Mount the column in the oven. Use the hanging bracket if available. Install the column nut and Vespel or graphite ferrule at each column end; pull the nut and ferrule down the tubing approximately 5 cm. (Table 1) Score (cut) the column. Use a light touch to score the column about 4 to 5 cm from each end. Make a clean break. Grasp the column between the thumb and forefinger as close to the score point as possible. Gently PULL and bend the column. The column should part easily. If the column doesn't break easily, don't force it! Score the column again in a different place, and try for a clean break. Use a magnifying loupe to inspect the cut. Make sure the cut is square across the tubing with no polyimide or glass fragments at the end of the tube. Install the column in the inlet. Check the GC manufacturer's instrument manual for the correct insertion distance. Mark the correct distance on the column with typewriter correction fluid. Insert the column into the injector. Finger tighten the column nut until it starts to grab the column, and then tighten the nut an additional 1/4 to 1/2 turn so that the column cannot be pulled from the fitting when gentle pressure is applied. Turn on the carrier gas and establish the proper flow rate. Set head pressure, split flow, and septum purge flow to appropriate levels. See Table 2 on page 325 for nominal head pressures. If using a split/splitless inlet, check that the purge (split) valve is ON (open).

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GC and GC/MS
Troubleshooting/Maintenance
Installing the Column (continued)
9. Confirm carrier gas flow through the column. Immerse the end of the column in a vial of solvent, and check for bubbles. 10. Install the column into the detector. Check the instrument manufacturer's manual for the proper insertion distance. 11. Check for leaks. THIS IS VERY IMPORTANT. Don't even THINK about heating the column without thoroughly checking for leaks. 12. Establish proper injector and detector temperatures. 13. Establish proper makeup and detector gas flows. Ignite or turn ON the detector. 14. Purge the column for a minimum of 10 minutes at ambient temperature. Add the appropriate additional purge time following inlet or trap maintenance. 15. Inject a nonretained substance to check for proper injector installation. Examples: butane or methane (FID), headspace vapors from acetonitrile (NPD), headspace vapors from methylene chloride (ECD), air (TCD), argon (mass spectrometer). Proper installation is indicated by a symmetrical peak. If tailing is observed, repeat the injector installation process.

Conditioning and Testing the Column


1. Condition the column at 20C above the maximum temperature of the analysis or at the maximum temperature of the column (whichever is lower) for 2 hours. If after 10 minutes at the upper temperature the background does not begin to fall, immediately cool the column and check for leaks. 2. If you are using Vespel or graphite/Vespel ferrules, recheck tightness after the conditioning process. 3. Confirm final proper average linear velocity by injecting a non-retained substance.

GC and GC/MS

Table 2. Approximate Head Pressures (psig)


Column Length (m) 10 12 15 20 25 30 40 50 60 75 105 20-40 40-60 30-45 20-30 6-10 8-14 4-8 5-10 7-15 10-20 20-30 15-25 10-20 3-5 2-4 0.18 5-10 10-15 8-12 5-10 1-2 0.2 Column ID (mm) 0.25 0.32 0.45 0.53

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GC and GC/MS
Troubleshooting/Maintenance Capillary GC Columns
On the most basic level, a capillary column is composed of two parts - the tubing and stationary phase (Figure 1). Fused silica (with an external polyimide coating) and stainless steel are the primary tubing materials. There are numerous stationary phases. Most are high molecular weight, thermally stable polymers that are liquids or gums. The most common stationary phases of this type are the polysiloxanes (sometimes incorrectly called silicones) and polyethylene glycols. The next most common type of stationary phases are small, porous particles composed of polymers or zeolites (e.g., alumina, molecular sieves).
Figure 1. GC Capillary Column

Stationary Phase Selection


1. If no information or ideas about which stationary phase to use is available, start with a DB-1 or DB-5. 2. Low bleed (ms) columns are usually more inert and have higher temperature limits. 3. Use the least polar stationary phase that provides satisfactory resolution and analysis times. Non-polar stationary phases have superior lifetimes to polar phases. 4. Use a stationary phase with a polarity similar to that of the solutes. This approach works more times than not; however, the best stationary phase is not always found using this technique. 5. If poorly separated solutes possess different dipoles or hydrogen bonding strengths, change to a stationary phase with a different amount (not necessarily more) of the dipole or hydrogen bonding interaction. Other co-elutions may occur upon changing the stationary phase, thus the new stationary phase may not provide better overall resolution. 6. If possible, avoid using a stationary phase that contains a functionality that generates a large response with a selective detector. For example, cyanopropyl containing stationary phases exhibit a disproportionately large baseline rise (due to column bleed) with NPDs. 7. A DB-1 or DB-5, DB-1701, DB-17 and DB-WAX cover the widest range of selectivities with the smallest number of columns. 8. PLOT columns are used for the analysis of gaseous samples at above ambient column temperatures.

GC and GC/MS

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GC and GC/MS
Troubleshooting/Maintenance Column Diameter Selection
1. Use 0.18-0.25 mm id columns when higher column efficiencies are needed. 0.18 mm id columns are especially well suited for GC/MS systems with low pumping capacities. Smaller diameter columns have the lowest capacities and require the highest head pressures. 2. Use 0.32 mm id columns when higher sample capacity is needed. They often provide better resolution of earlier eluting solutes for splitless injections or large injection volumes (>2 L) than 0.25 mm id columns. 3. Use 0.45 mm id columns when only a Megabore direct injector is available and higher column efficiency is desired. Well suited for high carrier gas flow rate situations such as with purge & trap and headspace samplers. 4. Use 0.53 mm id columns when only a Megabore direct injector is available. Well suited for high carrier gas flow rate situations such as with purge & trap and head-space samplers. 0.53 mm id columns have the highest sample capacities at constant df.

Column Length Selection


1. Start with 25-30 meter columns when the best length is unknown. 2. 10-15 meter columns are well suited for samples containing very well separated solutes or very few solutes. Shorter lengths are used for very small diameter columns to reduce head pressures. 3. 50-60 meters should be used when resolution is not possible by other means (smaller diameter, different stationary phase, change in column temperature). Best suited for complex samples containing a large number of solutes. Long columns have long analysis times and higher cost.

GC and GC/MS

Column Film Thickness Selection


1. For 0.18-0.32 mm id columns, a film thickness of 0.18-0.25 m is average or standard (i.e., not thin or thick) and used for most analyses. 2. For 0.45-0.53 mm id columns, a film thickness of 0.8-1.5 m is average or standard (i.e., not thin or thick) and used for most analyses. 3. Thick film columns are used to retain and resolve volatile solutes (e.g., light solvents, gases). Thick columns are more inert and have higher capacities. Thick film columns exhibit higher column bleed and decreased upper temperature limits. 4. Thin film columns are used to minimize the retention of high boiling, high molecular weight solutes (e.g., steroids, triglycerides). Thin columns are less inert and have lower capacities. Thin film columns exhibit lower column bleed.

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GC and GC/MS
Troubleshooting/Maintenance
Equation 1. Average Linear Velocity
u (cm/sec) = L tM

Carrier Gases
The carrier gas linear velocity or flow rate directly influences retention time and efficiency. The proper selection and setting of the carrier gas are essential to obtaining the best analysis times, efficiency and reproducibility. The carrier gas linear velocity or flow rate is controlled by adjusting the carrier gas pressure at the front of the column (commonly called the head pressure). The pressure setting is dependent on the type of carrier gas, the column length and diameter, column temperature, and the desired linear velocity or flow rate. For capillary columns, the average linear velocity ( ) is a better and more meaningful measure of the carrier gas than the flow rate (F). The average linear velocity can be thought of as the average speed of the carrier gas in cm/sec (i.e., centimeters of column traveled per second by a carrier gas molecule). The average linear velocity is calculated using Equation 1. Carrier gas linear velocities (and flow rates) are dependent on column temperature. At a constant head pressure, carrier gas linear velocities decrease as column temperature increases. This means the average linear velocity needs to be set at the same temperature for a given method if reproducible results are desired. Setting the average linear velocity at a different column temperature results in retention and resolution changes. Since the average linear velocity is dependent on column temperature, the velocity decreases during a temperature program run. Electronic pressure controlled injectors can be programmed to maintain a constant average linear velocity or flow rate throughout the temperature program. Using this feature may result in better resolution of the later eluting peaks or shorter analysis times. Contrary to common perception, hydrogen is a safe carrier gas. It is extremely diffusive in air and only explosive when air is present within a narrow concentration range. In other words, it is difficult to create a hydrogen explosion under GC conditions. Finally, safety features built into modern GCs greatly reduce any explosion possibilities and hazards.

L = tM =

column length (cm) retention time of an unretained peak (sec)

Recommended average linear velocities


He: 30-40 cm/sec H2: 50-80 cm/sec

Unretained Compounds
Detector Unretained Compound
FID ECD methane, butane1 methylene chloride2,3, dichlorodifluoromethane NPD acetonitrile4 TCD, MS methane, butane1, air PID, ELCD vinyl chloride 1. 2. From a disposable lighter. Place 1-2 drops in an autosampler vial and tightly cap. Shake and inject 1-2 L from the headspace of the vial. Do not inject any liquid. Use a column temperature above 50C. Use a column temperature above 90C.

GC and GC/MS

3. 4.

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GC and GC/MS
Troubleshooting/Maintenance Guard Columns or Retention Gap
A guard column and retention gap are the same thing, but they serve different purposes. Both are 1-10 meters of deactivated fused silica tubing attached to the front of the column. Deactivated fused silica tubing does not contain any stationary phase; however, the surface is deactivated to minimize solute interactions. A suitable union is used to attach the tubing to the column. In most cases, the diameter of the retention gap or guard column should be the same as the column. If the tubing sizes are different, it is better to have a larger diameter guard column or retention gap than a smaller one. Guard columns are used when samples contain nonvolatile residues that may contaminate a column. The nonvolatile residues deposit in the guard column and not in the column. This greatly reduces the interaction between the residues and the sample since the guard column does not retain the solutes (because it contains no stationary phase). Also, the residues do not coat the stationary phase which often results in poor peak shapes. Periodic cutting or trimming of the guard column is usually required upon a build-up of residues. Guard columns are often 5-10 meters in length to allow substantial trimming before the entire guard column has to replaced. The onset of peak shape problems is the usual indicator that the guard column needs trimming or changing. Retention gaps are used to improve peak shapes for some types of samples, columns, and GC conditions. Usually a minimum of 3-5 meters of tubing is required to obtain the benefits of a retention gap. The situations that benefit the most from retention gaps are large volume injections (>2 L) and solvent-stationary phase polarity mismatches for splitless, Megabore direct and on-column injections. Peak shapes are sometimes distorted when using combinations of these conditions. Polarity mismatches occur when the sample solvent and column stationary phase are very different in polarity. The greatest improvement is seen for the peaks eluting closest to the solvent front or solutes very similar to the solvent in polarity. The benefits of a retention gap are often unintentionally obtained when using a guard column.

GC and GC/MS

Detector Injector

Deactivated Fused Silica Tubing

Union Column

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GC and GC/MS
Troubleshooting/Maintenance

Causes of Column Performance Degradation


Column Breakage
Fused silica columns break wherever there is a weak point in the polyimide coating. The polyimide coating protects the fragile fused silica tubing. The continuous heating and cooling of the oven, vibrations caused by the oven fan, and being wound on a circular cage all place stress on the tubing. Eventually breakage occurs at a weak point. Weak spots are created when the polyimide coating is scratched or abraded. This usually occurs when a sharp point or edge is dragged over the tubing. Column hangers and tags, metal edges in the GC oven, column cutters, and miscellaneous items on the lab bench are just some of the common sources of sharp edges or points. It is rare for a column to spontaneously break. Column manufacturing practices tend to expose any weak tubing and eliminate it from use in finished columns. Larger diameter columns are more prone to breakage. This means that greater care and prevention against breakage must be taken with 0.45-0.53 mm id tubing than with 0.18-0.32 mm id tubing. A broken column is not always fatal. If a broken column was maintained at a high temperature either continuously or with multiple temperature program runs, damage to the column is very likely. The back half of the broken column has been exposed to oxygen at elevated temperatures which rapidly damages the stationary phase (see Thermal and Oxygen Damage on pages 330331). The front half is fine since carrier gas flowed through this length of column. If a broken column has not been heated or only exposed to high temperatures or oxygen for a very short time, the back half has probably not suffered any significant damage. A union can be installed to repair a broken column. Any suitable union will work to rejoin the column (see pages 164166). No more than 2-3 unions should be installed on any one column. Problems with dead volume (peak tailing) may occur with multiple unions.

GC and GC/MS

Thermal Damage
Exceeding a columns upper temperature limit results in accelerated degradation of the stationary phase and tubing surface. This results in the premature onset of excessive column bleed, peak tailing for active compounds and/or loss of efficiency (resolution). Fortunately, thermal damage is a slower process, thus prolonged times above the temperature limit are required before significant damage occurs. Thermal damage is greatly accelerated in the presence of oxygen. Overheating a column with a leak or high oxygen levels in the carrier gas results in rapid and permanent column damage. Setting the GCs maximum oven temperature at or a few degrees above the columns temperature limit is the best method to prevent thermal damage. This prevents the accidental overheating of the column. If a column is thermally damaged, it may still be functional. Remove the column from the detector. Heat the column for 8-16 hours at its isothermal temperature limit. Remove 10-15 cm from the detector end of the column. Reinstall the column and condition as usual. The column usually does not return to its original performance; however, it is often still functional. The life of the column will be reduced after thermal damage.

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GC and GC/MS
Troubleshooting/Maintenance Oxygen Damage
Oxygen is an enemy to most capillary GC columns. While no column damage occurs at or near ambient temperatures, severe damage occurs as the column temperature increases. In general, the temperature and oxygen concentration at which significant damage occurs is lower for polar stationary phases. It is constant exposure to oxygen that is the problem. Momentary exposure such as an injection of air or a very short duration septum nut removal is not a problem. A leak in the carrier gas flow path (e.g., gas lines, fittings, injector) is the most common source of oxygen exposure. As the column is heated, very rapid degradation of the stationary phase occurs. This results in the premature onset of excessive column bleed, peak tailing for active compounds and/or loss of efficiency (resolution). These are the same symptoms as for thermal damage. Unfortunately, by the time oxygen damage is discovered, significant column damage has already occurred. In less severe cases, the column may still be functional but at a reduced performance level. In more severe cases, the column is irreversibly damaged. Maintaining an oxygen and leak free system is the best prevention against oxygen damage. Good GC system maintenance includes periodic leak checks of the gas lines and regulators, regular septa changes, using high quality carrier gases, installing and changing oxygen traps, and changing gas cylinders before they are completely empty.

Chemical Damage
There are relatively few compounds that damage stationary phases. Introducing nonvolatile compounds (high molecular weight or high boiling point) in a column often degrades performance, but damage to the stationary phase does not occur. These residues can often be removed and performance returned by solvent rinsing the column (see next page for more information on column contamination and solvent rinsing). Inorganic or mineral bases and acids are the primary compounds to avoid introducing in a column. The acids include hydrochloric (HCl), sulfuric (H2SO4), nitric (HNO3), phosphoric (H3PO4) and chromic (CrO3). The bases include potassium hydroxide (KOH), sodium hydroxide (NaOH) and ammonium hydroxide (NH4OH). Most of these acids and bases are not very volatile and accumulate at the front of the column. If allowed to remain, the acids or bases damage the stationary phase. This results in the premature onset of excessive column bleed, peak tailing for active compounds and/or loss of efficiency (resolution). The symptoms are very similar to thermal and oxygen damage. Hydrochloric acid and ammonium hydroxide are the least harmful of the group. Both tend to follow any water that is present in the sample. If the water is not or only poorly retained by the column, the residence time of HCl and NH4OH in the column is short. This tends to eliminate or minimize any damage by these compounds. Thus, if HCl or NH4OH are present in a sample, using conditions or a column with no water retention will render these compounds relatively harmless to the column.

GC and GC/MS

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Troubleshooting/Maintenance
The only organic compounds that have been reported to damage stationary phases are perfluoroacids. Examples include trifluoroacetic, pentafluoropropanoic and heptafluorobutyric acid. They need to be present at high levels (e.g., 1% or higher). Most of the problems are experienced with splitless or Megabore direct injections where large volumes of the sample are deposited at the front of the column. Since chemical damage is usually limited to the front of the column, trimming or cutting 1/2-1 meter from the front of the column often eliminates any chromatographic problems. In more severe cases, 5 or more meters may need to be removed. The use of a guard column or retention gap will minimize the amount of column damage; however, frequent trimming of the guard column may be necessary. The acid or base often damages the surface of the deactivated fused silica tubing which leads to peak shape problems for active compounds.

Column Contamination
Column contamination is one of the most common problems encountered in capillary GC. Unfortunately, it mimics a very wide variety of problems and is often misdiagnosed as another problem. A contaminated column is usually not damaged, but it may be rendered unusable. There are two basic types of contaminants: nonvolatile and semivolatile. Nonvolatile contaminants or residues do not elute and accumulate in the column. The column becomes coated with these residues which interfere with the proper partitioning of solutes in and out of the stationary phase. Also, the residues may interact with active solutes resulting in peak adsorption problems (evident as peak tailing or loss of peak size). Active solutes are those containing a hydroxyl (-OH) or amine (-NH) group, and some thiols (-SH) and aldehydes. Semivolatile contaminants or residues accumulate in the column, but eventually elute. Hours to days may elapse before they completely leave the column. Like nonvolatile residues, they may cause peak shape and size problems and, in addition, are usually responsible for many baseline problems (instability, wander, drift, ghost peaks, etc.). Contaminants originate from a number of sources, with injected samples being the most common. Extracted samples are among the worst types. Biological fluids and tissues, soils, waste and ground water, and similar types of matrices contain high amounts of semivolatile and nonvolatile materials. Even with careful and thorough extraction procedures, small amounts of these materials are present in the injected sample. Several to hundreds of injections may be necessary before the accumulated residues cause problems. Injection techniques such as on-column, splitless and Megabore direct place a large amount of sample into the column, thus column contamination is more common with these injection techniques. Occasionally, contaminants originate from materials in gas lines and traps, ferrule and septa particles, or anything coming in contact with the sample (vials, solvents, syringes, pipettes, etc.). These types of contaminants are probably responsible when a contamination problem suddenly develops and similar samples in previous months or years did not cause any problems.

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Troubleshooting/Maintenance
Minimizing the amount of semivolatile and nonvolatile sample residues is the best method to reduce contamination problems. Unfortunately, the presence and identity of potential contaminants are often unknown. Rigorous and thorough sample cleanup is the best protection against contamination problems. The use of a guard column or retention gap (see page 329) often reduces the severity or delays the onset of column contamination induced problems. If a column becomes contaminated, it is best to solvent rinse the column to remove the contaminants. Maintaining a contaminated column at high temperatures for long periods of time (often called baking out a column) is not recommended. Baking out a column may convert some of the contaminating residues into insoluble materials that cannot be solvent rinsed from the column. If this occurs, the column cannot be salvaged in most cases. Sometimes the column can be cut in half and the back half may still be useable. Baking out a column should be limited to 1-2 hours at the isothermal temperature limit of the column.

Solvent Rinsing Columns


Solvent rinsing columns involves removing the column from the GC and passing milliliters of solvent through the column. Any residues soluble in the rinse solvents are washed from the column. Injecting large volumes of solvent while the column is still installed is not rinsing a column nor does it remove any contaminants from the column. A capillary GC column must have a bonded and cross-linked stationary phase before it can be solvent rinsed. Solvent rinsing a nonbonded stationary phase results in severe damage to the column. A column rinse kit is used to force solvent through the column (Figure 2). The rinse kit is attached to a pressurized gas source (N2 or He), and the column is inserted into the rinse kit. Solvent is added to the vial, and the vial is pressurized using the gas source. The pressure forces solvent to flow through the column. Residues dissolve into the solvent and are backflushed out of the column with the solvent. The solvent is then purged from the column, and the column is properly conditioned. Before rinsing a column, cut about 1/2 meter from the front (i.e., injector end) of the column. Insert the detector end of the column into the rinse kit. Multiple solvents are normally used to rinse columns. Each successive solvent must be miscible with the previous one. High boiling point solvents should be avoided especially as the last solvent. The sample solvent(s) is often a good choice. Methanol, methylene chloride and hexane are recommended and work very well for the majority of cases. Acetone can be substituted for methylene chloride to avoid using halogenated solvents; however, methylene chloride is one of the best rinsing solvents. If aqueous based samples (e.g., biological fluids and tissues) were injected, use water before the methanol. Some residues originating from aqueous based samples are only soluble in water and not organic solvents. Water and alcohols (e.g., methanol, ethanol, isopropanol) should be used to rinse bonded polyethylene glycol based stationary phases (e.g., DB-WAX, DBWAXetr, DB-FFAP, HP-Innowax) only as a last resort.

Table 3. Solvent Volumes for Rinsing Columns


Column ID (mm) 0.18-0.2 0.25 0.32 0.45 0.53 Solvent Volume (mL) 3-4 4-5 6-7

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7-8 10-12

Using larger volumes will not damage the column.

Figure 2. Solvent Rinse Kit

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Table 3 (page 333) lists the suggested solvent volumes for different diameter columns. Using larger sovent volumes is not harmful, but rarely better and merely wasteful. After adding the first solvent, pressurize the rinse kit, but stay below 20 psi. Use the highest pressure that keeps the solvent flow rate below 1 mL/min. Except for most 0.53 mm id columns, the rinse kit pressure will reach 20 psi before the flow rate reaches 1 mL/min. Longer rinse times are required when using heavy or viscous solvents, and for longer or smaller diameter columns. When all or most of the first solvent has entered the column, add the next solvent. The previous solvent does not have to vacate the column before the next solvent is started through the column. After the last solvent has left the column, allow the pressurizing gas to flow through the column for 5-10 minutes. Install the column in the injector and turn on the carrier gas. Allow the carrier gas to flow through the column for 5-10 minutes. Attach the column to the detector (or leave it unattached if preferred). Using a temperature program starting at 40-50C, heat the column at 2-3/min until the upper temperature limit of the column is reached. Maintain this temperature for 1-4 hours until the column is fully conditioned.

Column Storage
Capillary columns should be stored in their original box when removed from the GC. Place the GC septa over the ends to prevent debris from entering the tubing. Upon reinstallation of the column, the column ends need to be trimmed by 2-4 cm to ensure that a small piece of septa is not lodged in the column. If a column is left in a heated GC, there should always be carrier gas flow. The carrier gas flow can be turned off only if the oven, injector, detector and transfer lines are turned off (i.e., not heated). Without carrier gas flow, damage to the heated portion of the column occurs.

GC and GC/MS

Evaluating the Problem


The first step in any troubleshooting effort is to step back and evaluate the situation. Rushing to solve the problem often results in a critical piece of important information being overlooked or neglected. In addition to the problem, look for any other changes or differences in the chromatogram. Many problems are accompanied by other symptoms. Retention time shifts, altered baseline noise or drift, or peak shape changes are only a few of the other clues that often point to or narrow the list of possible causes. Finally, make note of any changes or differences involving the sample. Solvents, vials, pipettes, storage conditions, sample age, extraction, preparation techniques, or any other factor influencing the sample environment can be responsible.

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Troubleshooting/Maintenance Checking the Obvious
A surprising number of problems involve fairly simple and often overlooked components of the GC system or analysis. Many of these items are transparent in the daily operation of the GC and are often taken for granted (set it and forget it). The areas and items to check include: 1. Gases - pressures, carrier gas average linear velocity, and flow rates (detector, split vent, septum purge). 2. Temperatures - column, injector, detector and transfer lines. 3. System parameters - purge activation times, detector attenuation and range, mass ranges, etc. 4. Gas lines and traps - cleanliness, leaks, and expiration. 5. Injector consumables - septa, liners, O-rings and ferrules. 6. Sample integrity - concentration, degradation, solvent, and storage. 7. Syringes - handling technique, leaks, needle sharpness, and cleanliness. 8. Data system - settings and connections.

The Most Common Problems


Ghost Peaks or Carryover
System contamination is responsible for most ghost peaks or carryover problems. If the extra ghost peaks are similar in width to the sample peaks (with similar retention times), the contaminants were most likely introduced into the column at the same time as the sample. The extra compounds may be present in the injector (i.e., contamination) or in the sample itself. Impurities in solvents, vials, caps and syringes are only some of the possible sources. Injecting sample and solvent blanks may help to find possible sources of the contaminants. If the ghost peaks are much broader than the sample peaks, the contaminants were most likely already in the column when the injection was made. These compounds were still in the column when a previous GC run was terminated. They elute during a later run and are often very broad. Sometimes numerous ghost peaks from multiple injections overlap and elute as a hump or blob. This often takes on the appearance of baseline drift or wander. Increasing the final temperature or time in the temperature program is one method to minimize or eliminate a ghost peak problem. Alternatively, a short bake-out after each run or series of runs may remove the highly retained compounds from the column before they cause a problem. Performing a condensation test is a good method to determine whether a contaminated injector is the source of the carryover or ghost peaks.

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Condensation Test
Use this test whenever injector or carrier gas contamination problems are suspected (e.g., ghost peaks or erratic baselines). 1. Leave the GC at 40-50C for 8 or more hours. 2. Run a blank analysis (i.e., start the GC, but with no injection) using the normal temperature conditions and instrument settings. 3. Collect the chromatogram for this blank run. 4. Immediately repeat the blank run as soon as the first one is completed. Do not allow more than 5 minutes to elapse before starting the second blank run. 5. Collect the chromatogram for the second blank run and compare it to the first chromatogram. 6. If the second chromatogram contains a substantially larger amount of peaks and baseline instability, the incoming carrier gas line or the carrier gas is contaminated. 7. If the second chromatogram contains few peaks or very little baseline drift, the carrier gas and incoming carrier gas lines are relatively clean.

Excessive Baseline Noise


Possible Cause Injector contamination Column contamination Solution Bake-out the column Solvent rinse the column Comments Limit the bake-out to 1-2 hours Only for bonded and cross-linked phases Check for inlet contamination Detector contamination Contaminated or low quality gases Column inserted too far into the detector Incorrect detector gas flow rates Leak when using an MS, ECD or TCD Old detector filament, lamp or electron multiplier Septum degradation Clean the detector Use better grade gases; also check for expired gas traps or leaks Reinstall the column Adjust the flow rates to the recommended values Find and eliminate the leak Replace appropriate part Replace septum For high temperature applications use an appropriate septum Usually the noise increases over time and not suddenly Usually occurs after changing a gas cylinder Consult GC manual for the proper insertion distance Consult the GC manual for the proper flow rates Usually at the column fittings or injector Clean the injector, replace liner, gold seal Try a condensation test; gas lines may also need cleaning

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Baseline Instability or Disturbances
Possible Cause Injector contamination Column contamination Unequilibrated detector Incompletely conditioned column Change in carrier gas flow rate during the temperature program Solution Clean the injector Bake-out the column Allow the detector to stabilize Fully condition the column Normal in many cases Comments Try a condensation test; gas lines may also need cleaning Limit a bake-out to 1-2 hours Some detectors may require up to 24 hours to fully stabilize More critical for trace level analyses MS, TCD and ECD respond to changes in carrier gas flow rate

Baseline Instability or Disturbances


Possible Cause Column contamination Solution Trim the column Solvent rinse the column Column activity Solvent-phase polarity mismatch Irreversible. Replace the column Change sample solvent Install a retention gap Solvent effect violation for splitless or on-column injections Too low of a split ratio Poor column installation Some active compounds always tail Decrease the initial column temperature Increase the split ratio Reinstall the column None Comments Remove 1/2-1 meter from the front of the column Only for bonded and cross-linked phases Check the inlet for contamination Only affects active compounds More tailing for the early eluting peaks or those closest to the solvent front 3-5 meter retention gap is sufficient Peak tailing decreases with retention

GC and GC/MS

Flow from split vent should be 20 mL/min or higher More tailing for the early eluting peaks Most common for amines and carboxylic acids

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Split Peaks
Possible Cause Injection technique Mixed sample solvent to a single solvent Poor column installation Sample degradation in the injector Solution Change technique Change the sample solvent Reinstall the column in the injector Reduce the injector temperature Change to an on-column injection Poor sample focusing Use a retention gap Comments Usually related to erratic plunger depression or having sample in the syringe needle. Use an auto injector. Worse for solvents with large differences in polarity or boiling points Usually a large error in the insertion distance Peak broadening or tailing may occur if the temperature is too low. Requires an on-column injector For splitless and on-column injection

Retention Time Shift


Possible Cause Change in carrier gas velocity Change in column temperature Change in column dimension Large change in compound concentration Solution Check the carrier gas velocity Check the column temperature Verify column identity Try a different sample concentration Leak check the injector Clean or replace the plugged line Replace the septum Change solvent, use a retention gap May also affect adjacent peaks. Sample overloading is corrected with an increase split ratio or sample dilution A change in peak size usually occurs also More common for the split line; also check flow controllers and solenoids Check for needle barb For splitless injection Comments All peaks will shift in the same direction by approximately the same amount Not all peaks will shift by the same amount

GC and GC/MS

Leak in the injector Blockage in a gas line Septum leak Sample solvent incompatibility

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Troubleshooting/Maintenance
Change in Peak Size
Possible Cause Change in detector response Solution Check gas flows, temperatures and settings Check background level or noise Change in the split ratio Change in the purge activation time Change in injection volume Change in sample concentration Leak in the syringe Column contamination Column activity Coelution Change in injector discrimination Sample flashback Decomposition from inlet contamination Check split ratio Check the purge activation time Check the injection technique Check and verify sample concentration Use a different syringe Trim the column Solvent rinse the column Irreversible Change column temperature or stationary phase Maintain the same injector parameters Inject less, use a larger liner, reduce the inlet temperature Clean the inlet, replace the liner, replace the gold seal Comments All peaks may not be equally affected May be caused by system contamination and not the detector All peaks will not be equally affected For splitless injectors Injection volumes are not linear Changes may also be caused by degradation, evaporation, or variances in sample temperature or pH Sample leaks passed the plunger or around the needle; leaks are often not readily visible Remove 1/2-1 meter from the front of the column Only for bonded and cross-linked phases Only affects active compounds Decrease column temperature and check for the appearance of a peak shoulder or tail Most severe for split injections

GC and GC/MS

Less solvent and higher flow rates are most helpful Only use deactivated liners and glass wool in the inlet

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Loss of Resolution
Possible Cause Decrease in separation Different column temperature Different column dimensions or phase Coelution with another peak Increase in peak width Change in carrier gas velocity Column contamination Change in the injector Change in sample concentration or solvent Improper solvent effect, lack of focusing Solution Check column temperature Verify column identity Change the column temperature Check carrier gas velocity Trim the column Solvent rinse the column Check the injector settings Try a different sample concentration Lower oven temperature, better solvent, sample phase polarity match, use a retention gap Comments Differences in other peaks will be visible Differences in other peaks will be visible Decrease column temperature and check for the appearance of a peak shoulder or tail A change in retention time also occurs Remove 1/2-1 meter from the front of the column Only for bonded and cross-linked phases Typical areas: split ratio, liner, temperature, injection volume Peak widths increase at higher concentrations For splitless injectors

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Solvent Retention Data

Solvent Retention Data


DB-624 - Alphabetical Order
Column: P/N: Oven: DB-624 30 m x 0.53 mm id, 3 m 125-1334 40C for 5 min 40-260C at 10/min 260C for 3 min Helium, constant pressure at 30 cm/sec (40C) Split 1:10, 250C FID, 300C

Retention Time 20.02 13.43 10.37 18.69 17.96 18.79 16.46 6.11 9.62 4.00 7.16 5.33 8.72 10.17 12.86 7.16 8.34 11.38 12.30 18.24 21.46 18.04 16.78 9.92 20.35 20.73 12.94 17.06 10.38 7.30 13.73 15.58 20.58 11.22 3.47 10.98 15.57 7.34 10.02 14.42 21.22 12.15 15.84 10.95 8.64 3.72 4.27 18.40 22.65 23.03 10.37 3.72 9.18 3.24 3.72 14.63 15.32 11.93 8.65 15.60 9.15 22.97 17.22 15.63 15.53 15.46 15.29 14.95 17.22 17.22 21.69 26.63 13.14 5.82 21.30 15.06 13.31 13.16 13.01 12.80 15.19 14.88 14.24 14.98 18.10 4.27

Compound 1,2-dibromo-3-chloropropane (DBCP) 1,2-dibromoethane (EDB) dibromomethane 1,2-dichlorobenzene 1,3-dichlorobenzene 1,4-dichlorobenzene trans-1,4-dichloro-2-butene 1,1-dichloroethane 1,2-dichloroethane (ethylene dichloride) 1,1-dichloroethylene (vinylidine chloride) cis-1,2-dichloroethylene trans-1,2-dichloroethylene 1,1-dichloropropane 1,2-dichloropropane 1,3-dichloropropane 2,2-dichloropropane 1,1-dichloropropene cis-1,3-dichloropropene trans-1,3-dichloropropene diethylene glycol diethylene glycol monobutyl ether diethylene glycol monoethyl ether diethylene glycol monomethyl ether diglyme (diethylene glycol dimethyl ether) 1,3-diisopropylbenzene 1,4-diisopropylbenzene 2,4-dimethyl-3-pentanone (diisopropyl ketone) 2,6-dimethyl-4-heptanone (diisobutyl ketone) 1,4-dioxane 1,3-dioxolane DMF (dimethylformamide) DMSO (methyl sulfoxide) dodecane epichlorohydrin ethanol 2-ethoxyethanol (cellosolve) 2-ethoxyethyl acetate ethyl acetate ethyl acrylate ethylbenzene ethyl benzoate ethylene glycol ethylene glycol monobutyl ether ethylene glycol monoethyl ether ethylene glycol monomethyl ether ethyl ether ethyl formate 2-ethyl-1-hexanol -ethylphenethyl alcohol -ethylphenethyl alcohol ethyl propionate ethyl vinyl ether fluorobenzene fluorotrichloromethane (Freon 11) furan furfural furfuryl alcohol glycidol glyme (propylene glycol dimethyl ether) heptanal heptane 1,7-heptanediol 1-heptanol 2-heptanol 3-heptanol 2-heptanone 3-heptanone 4-heptanone trans-2-hepten-1-ol cis-4-hepten-1-ol hexachloro-1,3-butadiene hexadecane hexanal hexane 1,6-hexanediol 1-hexanol 2-hexanol 3-hexanol 2-hexanone 3-hexanone cis-2-hexen-1-ol cis-3-hexen-1-ol 4-hexen-3-one 4-hydroxy-4-methyl-2-pentanone iodobenzene iodomethane

Retention Time 14.75 8.60 12.19 17.68 5.66 8.81 21.04 4.27 8.87 15.88 6.23 6.01 7.53 2.59 8.67 4.60 19.90 11.87 8.73 11.78 9.21 7.91 12.85 14.82 4.80 2.80 16.52 14.73 11.85 11.64 12.28 13.20 19.60 7.88 19.66 17.22 17.68 5.30 12.98 20.35 21.60 22.40 22.79 19.71 14.63 26.02 20.97 17.76 12.11 24.54 19.17 17.63 17.45 27.10 25.26 10.25 3.37 19.52 10.34 12.63 10.60 10.60 10.11 10.34 10.20 12.63 10.03 6.93 18.53 22.65 22.91 13.13 6.34 6.21 3.91 11.89 7.25 10.51 16.56 22.92 13.16 9.05 7.66 13.07 7.55 11.70

Compound iso-amyl acetate iso-butanol iso-butyl acetate iso-butylbenzene iso-butyraldehyde iso-octane isophorone iso-propanol iso-propyl acetate iso-propylbenzene (cumene) iso-propyl ether methacrolein methacrylonitrile methanol 2-methoxyethanol (methyl cellosolve) methyl acetate methyl benzoate 2-methyl-1-butanol (active amyl alcohol) 2-methyl-2-butanol (tert-amyl alcohol) 3-methyl-1-butanol (iso-amyl alcohol) 3-methyl-2-butanone 2-methyl-3-buten-2-ol 3-methyl-2-buten-1-ol 2-methylbutyl acetate methylene chloride methyl formate 5-methyl-3-heptanone 5-methyl-2-hexanone 2-methyl-3-pentanone 4-methyl-2-pentanone(MIBK; methyl isobutyl ketone) 4-methyl-2-pentanol 4-methyl-3-penten-3-one -methylphenyl alcohol methyl propionate 1-methylpyrrolidone -methylstyrene 4-methylstyrene methyl tert-butyl ether (MTBE) morpholine nitrobenzene 2-nitrotoluene 3-nitrotoluene 4-nitrotoluene nonanal nonane 1,9-nonanediol 1-nonanol octanal octane 1,8-octanediol 1-octanol 2-octanone 3-octanone pentachlorobenzene pentadecane pentanal (valeraldehyde) pentane 1,5-pentanediol 2,3-pentanedione 1-pentanol 2-pentanol 3-pentanol 2-pentanone 3-pentanone 1-penten-3-ol 2-penten-1-ol 1-penten-3-one (ethyl vinyl ketone) 3-penten-2-one (methyl vinyl ketone) pentyl ether 2-phenoxyethanol 4-phenyl-2-butanone (benzyl acetone) 1,3-propanediol 1-propanol 2-propen-1-ol (allyl alcohol) propionaldehyde propionic acid propionitrile propyl acetate propylbenzene propyl benzoate propylene glycol (1,2-propanediol) propyl ether propyl formate propyl propionate 2-propyn-1-ol (propargyl alcohol) pyridine

Carrier: Injector: Detector:

Retention Time 10.58 2.46 9.10 4.05 4.27 19.69 3.81 12.21 5.22 9.65 6.41 15.57 17.45 8.69 18.21 21.07 19.27 29.08 16.39 7.59 10.64 4.27 15.61 16.10 17.70 14.14 6.99 9.73 7.19 9.87 9.02 15.89 13.24 18.69 14.41 9.34 7.10 6.84 4.27 8.34 14.25 8.25 13.25 7.75 14.21 23.06 4.57 19.35 16.71 16.82 16.82 9.18 8.10 15.63 16.04 13.16 13.39 16.82 27.43 22.65

Compound acetal (acetaldehyde diethyl acetal) acetaldeyde acetic acid acetone acetonitrile acetophenone acrolein acrylic acid acrylonitrile allyl ether allyl ethyl ether amyl acetate benzaldehyde benzene benzonitrile benzyl acetate benzyl alcohol benzyl ether bromobenzene bromochloromethane bromodichloromethane bromoethane bromoform 1,3-butanediol 1,4-butanediol 2,3-butanediol 2,3-butanedione (diacetal) 1-butanol 2-butanone (MEK) 2-buten-1-ol (crotyl alcohol) 3-buten-1-ol 2-butoxyethanol (butyl cellosolve) butyl acetate butylbenzene butyl ether butyl ethyl ether butyl methyl ether butyraldehyde carbon disulfide carbon tetrachloride chlorobenzene 1-chlorobutane chlorodibromomethane chloroform 1-chlorohexane 1-chloro-4-nitrobenzene 3-chloropropene (allyl chloride) 4-chlorostyrene 2-chlorotoluene 3-chlorotoluene 4-chlorotoluene crotonaldehyde cyclohexane cyclohexanol cyclohexanone cyclopentanol cyclopentanone decane 1,10-decanediol 1-decanol

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Solvent Retention Data
Retention Time 7.55 11.76 17.68 15.28 19.46 8.89 5.01 10.02 17.39 6.90 19.35 24.02 14.38 16.38 12.86 23.80 10.06 7.64 19.63 19.63 19.96 11.93 22.23 21.41 20.35 8.05 12.60 9.80 16.46 4.00 22.24 8.91 21.95 18.18 17.47 16.82 18.78 6.37 14.62 15.28 14.62 Compound sec-butanol sec-butyl acetate sec-butylbenzene styrene styrene oxide tert-amyl methyl ether tert-butanol tert-butyl acetate tert-butylbenzene tert-butyl ethyl ether 4-tert-butyltoluene 1,2,4,5-tetrachlorobenzene 1,1,1,2-tetrachloroethane 1,1,2,2-tetrachloroethane tetrachloroethylene tetradecane tetrahydropyran THF (tetrahydrofuran) m-tolualdehyde o-tolualdehyde p-tolualdehyde toluene 1,2,3-trichlorobenzene 1,2,4-trichlorobenzene 1,3,5-trichlorobenzene 1,1,1-trichloroethane 1,1,2-trichloroethane trichloroethylene 1,2,3-trichloropropane 1,1,2-trichlorotrifluoroethane (Freon 113) tridecane triethylamine triglyme (triethylene glycol dimethyl ether) 1,2,3-trimethylbenzene (hemimellitene) 1,2,4-trimethylbenzene (pseudocumene) 1,3,5-trimethylbenzene (mesitylene) undecane vinyl acetate m-xylene o-xylene p-xylene Retention Time 13.30 15.03 11.40 5.09 7.90 5.41 7.99 6.95 14.72 12.36 14.39 9.18 6.85 18.24 5.29 4.37 4.09 8.18 13.44 7.56 11.81 6.34 13.69 21.34 3.96 18.56 16.07 16.07 16.07 7.03 8.32 14.26 14.26 11.56 11.42 11.90 16.26 17.12 25.84 21.85 18.50 12.10 8.93 17.81 17.20 17.31 14.91 5.02 7.17 3.75 5.98 6.17 8.55 8.93 11.35 6.34 7.85 10.20 10.80 15.60 20.26 16.60 15.09 8.68 20.02 20.42 10.80 11.94 9.18 4.09 20.85 9.35 2.68 9.39 14.40 6.21 8.93 13.90 20.15 8.54 14.68 9.35 6.72 3.50 3.56 17.42 21.38 Compound 1,3-butanediol 1,4-butanediol 2,3-butanediol 2,3-butanedione (diacetal) 1-butanol 2-butanone (MEK; methyl ethyl ketone) 2-buten-1-ol (crotyl alcohol) 3-buten-1-ol 2-butoxyethanol (butyl cellosolve) butyl acetate butyl ether butyl ethyl ether butyl methyl ether butylbenzene butyraldehyde iso-butyraldehyde carbon disulfide carbon tetrachloride chlorobenzene 1-chlorobutane chlorodibromomethane chloroform 1-chlorohexane 1-chloro-4-nitrobenzene 3-chloropropene (allyl chloride) 4-chlorostyrene 2-chlorotoluene 3-chlorotoluene 4-chlorotoluene crotonaldehyde cyclohexane cyclohexanol cyclohexanone cyclopentanol cyclopentanone 2,4-dimethyl-3-pentanone (diisopropyl ketone) 2,6-dimethyl-4-heptanone (diisobutyl ketone) decane 1,10-decanediol 1-decanol 1,2-dibromo-3-chloropropane (DBCP) 1,2-dibromoethane (EDB) dibromomethane 1,2-dichlorobenzene 1,3-dichlorobenzene 1,4-dichlorobenzene trans-1,4-dichloro-2-butene 1,1-dichloroethane 1,2-dichloroethane (ethylene dichloride) 1,1-dichloroethylene (vinylidine chloride) cis-1,2-dichloroethylene trans-1,2-dichloroethylene 1,1-dichloropropane 1,2-dichloropropane 1,3-dichloropropane 2,2-dichloropropane 1,1-dichloropropene cis-1,3-dichloropropene trans-1,3-dichloropropene diethylene glycol diethylene glycol monobutyl ether diethylene glycol monoethyl ether diethylene glycol monomethyl ether diglyme (diethylene glycol dimethyl ether) 1,3-diisopropylbenzene 1,4-diisopropylbenzene DMF (dimethylformamide) DMSO (methylsulfoxide) 1,4-dioxane 1,3-dioxolane dodecane epichlorohydrin ethanol 2-ethoxyethanol (cellosolve) 2-ethoxyethyl acetate ethyl acetate ethyl acrylate ethylbenzene ethyl benzoate ethylene glycol ethylene glycol monobutyl ether ethylene glycol monoethyl ether ethylene glycol monomethyl ether ethyl ether ethyl formate 2-ethyl-1-hexanol -ethylphenethyl alcohol Retention Time 21.71 9.42 3.50 8.36 3.23 3.36 12.54 13.25 9.10 7.56 14.52 9.58 21.03 16.13 14.61 14.54 14.26 14.19 13.88 16.13 16.02 21.46 26.88 11.88 6.25 19.17 13.81 12.06 11.97 11.60 11.52 13.81 13.51 12.76 12.89 17.87 3.75 13.97 6.74 11.30 17.31 9.27 19.31 3.22 7.88 6.21 15.43 4.68 5.36 2.15 6.74 3.79 18.76 10.30 7.14 10.17 7.60 6.17 11.33 14.81 3.85 2.44 15.55 13.46 10.59 10.22 10.93 11.90 18.03 6.78 17.21 16.62 16.99 5.06 13.62 18.84 14.95 24.33 20.11 18.56 20.02 20.72 21.05 16.80 12.48 22.73 18.21 Compound -ethylphenethyl alcohol ethyl propionate ethyl vinyl ether fluorobenzene fluorotrichloromethane (Freon 11) furan furfural furfuryl alcohol glycidol glyme (propylene glycol dimethyl ether) heptanal heptane 1,7-heptanediol 1-heptanol 2-heptanol 3-heptanol 2-heptanone 3-heptanone 4-heptanone trans-2-hepten-1-ol cis-4-hepten-1-ol hexachloro-1,3-butadiene hexadecane hexanal hexane 1,6-hexanediol 1-hexanol 2-hexanol 3-hexanol 2-hexanone 3-hexanone cis-2-hexen-1-ol cis-3-hexen-1-ol 4-hexen-3-one 4-hydroxy-4-methyl-2-pentanone iodobenzene iodomethane iso-amyl acetate iso-butanol iso-butyl acetate iso-butylbenzene iso-octane isophorone iso-propanol iso-propyl acetate iso-propyl ether iso-propylbenzene (cumene) methacrolein methacrylonitrile methanol 2-methoxyethanol (methyl cellosolve) methyl acetate methyl benzoate 2-methyl-1-butanol (active amyl alcohol) 2-methyl-2-butanol (tert-amyl alcohol) 3-methyl-1-butanol (iso-amyl alcohol) 3-methyl-2-butanone 2-methyl-3-buten-2-ol 3-methyl-2-buten-1-ol 2-methylbutyl acetate methylene chloride methyl formate 5-methyl-3-heptanone 5-methyl-2-hexanone 2-methyl-3-pentanone 4-methyl-2-pentanone (MIBK; methyl isobutyl ketone) 4-methyl-2-pentanol 4-methyl-3-penten-3-one -methylphenyl alcohol methyl propionate 1-methyl-2-pyrrolidone -methylstyrene 4-methylstyrene methyl tert-butyl ether (MTBE) morpholine nonanal nonane 1,9-nonanediol 1-nonanol nitrobenzene 2-nitrotoluene 3-nitrotoluene 4-nitrotoluene octanal octane 1,8-octanediol 1-octanol

GC and GC/MS

DB-1 - Alphabetical Order


Column: P/N: Oven: Carrier: Injector: Detector: DB-1 30 m x 0.53 mm id, 3 m 125-1034 40C for 5 min 40-260C at 10/min Helium, constant pressure at 30 cm/sec (40C) Split 1:10, 250C FID, 300C

Retention Time 10.21 2.16 16.87 3.05 2.87 18.13 2.98 19.16 3.43 9.05 6.00 14.04 15.88 8.00 16.26 17.42 27.72 15.54 4.79 9.22 3.75 14.20

Compound acetal (acetaldehyde diethyl acetal) acetaldehyde acetic acid acetone acetonitrile acetophenone acrolein acrylic acid acrylonitrile allyl ether allyl ethyl ether amyl acetate benzaldehyde benzene benzyl acetate benzyl alcohol benzyl ether bromobenzene bromochloromethane bromodichloromethane bromoethane bromoform

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Solvent Retention Data
Retention Time 16.46 16.46 26.38 25.51 8.76 3.51 17.17 8.66 11.11 9.10 9.10 8.46 8.80 8.54 11.11 8.40 5.09 18.51 20.92 21.36 9.95 4.44 4.09 3.11 18.18 4.43 4.57 9.47 16.07 21.91 9.05 9.90 6.48 12.25 10.21 5.80 10.91 17.37 14.55 18.24 8.68 3.72 9.32 16.99 6.85 18.97 23.35 13.43 14.67 12.66 24.06 9.35 6.75 18.23 18.23 18.50 11.33 21.28 20.63 19.85 7.56 11.00 9.22 14.84 4.09 22.51 8.93 20.75 17.61 16.99 16.41 19.07 4.09 14.11 14.69 14.11 Compound 2-octanone 3-octanone pentachlorobenzene pentadecane (valeraldehyde) pentane 1,5-pentanediol 2,3-pentanedione 1-pentanol 2-pentanol 3-pentanol 2-pentanone 3-pentanone 1-penten-3-ol 2-penten-1-ol 1-penten-3-one (ethyl vinyl ketone) 3-penten-2-one (methyl vinyl ketone) pentyl ether 2-phenoxyethanol 4-phenyl-2-butanone (benzyl acetone) 1,3-propanediol 1-propanol 2-propen-1-ol (allyl alcohol) propionaldehyde propionic acid propionitrile 2-propyn-1-ol (propargyl alcohol) propyl acetate propylbenzene propyl benzoate propyl ether propylene glycol (1,2-propanediol) propyl formate propyl propionate pyridine sec-butanol sec-butyl acetate sec-butylbenzene styrene styrene oxide tert-amyl methyl ether tert-butanol tert-butyl acetate tert-butylbenzene tert-butyl ethyl ether 4-tert-butyltoluene 1,2,4,5-tetrachlorobenzene 1,1,1,2-tetrachloroethane 1,1,2,2-tetrachloroethane tetrachloroethylene tetradecane tetrahydropyran THF (tetrahydrofuran) m-tolualdehyde o-tolualdehyde p-tolualdehyde toluene 1,2,3-trichlorobenzene 1,2,4-trichlorobenzene 1,3,5-trichlorobenzene 1,1,1-trichloroethane 1,1,2-trichloroethane trichloroethylene 1,2,3-trichloropropane 1,1,2-trichlorotrifluoroethane (Freon 113) tridecane triethylamine triglyme (triethylene glycol dimethyl ether) 1,2,3-trimethylbenzene (hemimellitene) 1,2,4-trimethylbenzene (pseudocumene) 1,3,5-trimethylbenzene (mesitylene) undecane vinyl acetate m-xylene o-xylene p-xylene

DB-WAX - Alphabetical Order


Column: P/N: Oven: DB-WAX 30 m x 0.53 mm id, 1 m 125-7032 40C for 5 min 40-230C at 10/min 230C for 7 min Helium, constant pressure at 34 cm/sec (40C) Split 1:10, 250C FID, 300C

Retention Time 17.06 17.00 9.10 7.20 2.65 7.84 4.38 9.71 4.95 12.38 3.99 4.56 11.27 12.78 23.91 21.74 19.48 19.06 6.04 15.47 16.20 7.94 12.03 9.65 7.09 15.25 19.21 12.23 11.69 6.46 13.11 14.31 5.03 7.87 11.13 20.27 19.47 16.24 13.11 12.24 2.13 3.78 17.41 23.10 24.12 6.93 2.39 7.72 2.13 3.27 18.32 19.90 17.25 6.04 12.32 2.65 27.26 16.91 14.71 14.29 12.27 11.66 11.06 17.77 17.67 17.84 18.70 10.07 25.75 2.05 15.31 12.95 12.49 10.04 9.34 16.31 15.87 12.55 15.70 18.30 3.46 10.96 10.31 8.36 13.47 3.54 2.44

Compound 1,4-dichlorobenzene trans-1,4-dichloro-2-butene 1,1-dichloroethane 1,2-dichloroethane (ethylene dichloride) 1,1-dichloroethylene (vinylidine chloride) cis-1,2-dichloroethylene trans-1,2-dichloroethylene 1,1-dichloropropane 1,2-dichloropropane 1,3-dichloropropane 2,2-dichloropropane 1,1-dichloropropene cis-1,3-dichloropropene trans-1,3-dichloropropene diethylene glycol diethylene glycol monobutyl ether diethylene gycol monoethyl ether diethylene glycol monomethyl ether diglyme (diethylene glycol dimethyl ether) 1,3-diisopropylbenzene 1,4-diisopropylbenzene 2,4-dimethyl-3-pentanone (diisopropyl ketone) 2,6-dimethyl-4-heptanone (diisobutyl ketone) 1,4-dioxane 1,3-dioxolane DMF (dimethylformamide) DMSO (methyl sulfoxide) dodecane epichlorohydrin ethanol 2-ethoxyethanol (cellosolve) 2-ethoxyethyl acetate ethyl acetate ethyl acrylate ethylbenzene ethyl benzoate ethylene glycol ethylene glycol monobutyl ether ethylene glycol monoethyl ether ethylene glycol monomethyl ether ethyl ether ethyl formate 2-ethyl-1-hexanol -ethylphenethyl alcohol -ethylphenethyl alcohol ethyl propionate ethyl vinyl ether fluorobenzene fluorotrichloromethane (Freon 11) furan furfural furfuryl alcohol glycidol glyme (propylene glycol dimethyl ether) heptanal heptane 1,7-heptanediol 1-heptanol 2-heptanol 3-heptanol 2-heptanone 3-heptanone 4-heptanone trans-2-hepten-1-ol cis-4-hepten-1-ol hexachloro-1,3-butadiene hexadecane hexanal 1,6-hexanediol hexane 1-hexanol 2-hexanol 3-hexanol 2-hexanone 3-hexanone cis-2-hexen-1-ol cis-3-hexen-1-ol 4-hexen-3-one 4-hydroxy-4-methyl-2-pentanone iodobenzene iodomethane iso-amyl acetate iso-butanol iso-butyl acetate iso-butylbenzene iso-butyraldehyde iso-octane

Carrier: Injector: Detector:

Retention Time 5.09 2.47 16.87 3.60 8.12 20.13 4.10 19.61 7.81 6.04 3.05 10.96 17.25 6.46 19.55 21.01 22.82 30.41 15.47 9.26 11.76 2.95 17.00 20.96 23.14 18.70 7.44 11.48 5.35 12.95 12.02 16.31 9.85 14.81 6.97 3.27 2.80 4.72 2.65 4.85 13.00 3.99 14.52 8.58 9.10 23.97 3.46 18.19 14.71 14.90 14.90 9.07 2.27 16.31 14.61 14.57 12.46 7.63 34.11 21.12 20.57 13.80 11.98 17.80 16.60

Compound acetal (acetaldehyde diethyl acetal) acetaldeyde acetic acid acetone acetonitrile acetophenone acrolein acrylic acid acrylonitrile allyl ether allyl ethyl ether amyl acetate benzaldehyde benzene benzonitrile benzyl acetate benzyl alcohol benzyl ether bromobenzene bromochloromethane bromodichloromethane bromoethane bromoform 1,3-butanediol 1,4-butanediol 2,3-butanediol 2,3-butanedione (diacetal) 1-butanol 2-butanone (MEK; methyl ethyl ketone) 2-buten-1-ol (crotyl alcohol) 3-buten-1-ol 2-butoxyethanol (butyl cellosolve) butyl acetate butylbenzene butyl ether butyl ethyl ether butyl methyl ether butyraldehyde carbon disulfide carbon tetrachloride chlorobenzene 1-chlorobutane chlorodibromomethane chloroform 1-chlorohexane 1-chloro-4-nitrobenzene 3-chloropropene (allyl chloride) 4-chlorostyrene 2-chlorotoluene 3-chlorotoluene 4-chlorotoluene crotonaldehyde cyclohexane cyclohexanol cyclohexanone cyclopentanol cyclopentanone decane 1,10-decanediol 1-decanol 1,2-dibromo-3-chloropropane (DBCP) 1,2-dibromoethane (EDB) dibromomethane 1,2-dichlorobenzene 1,3-dichlorobenzene

GC and GC/MS

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Solvent Retention Data
Retention Time 19.47 6.28 5.32 12.13 3.27 4.83 7.54 5.40 12.25 3.78 19.70 12.73 8.43 12.73 6.15 9.11 14.82 12.05 6.18 2.85 12.38 11.40 7.94 8.19 11.89 11.26 22.00 5.54 15.13 15.61 20.71 2.30 13.62 21.41 22.62 21.92 23.14 16.05 4.97 31.29 19.80 14.29 3.22 29.06 18.40 14.20 13.66 25.09 17.28 7.46 1.89 24.43 9.49 13.54 10.94 10.66 7.44 7.44 11.77 14.65 8.56 6.61 12.66 26.14 22.76 18.97 9.11 10.78 3.25 18.18 8.72 7.38 12.86 21.46 18.96 3.05 5.93 9.17 15.16 12.44 8.77 7.69 13.64 13.80 19.46 3.27 5.54 Compound isophorone iso-propanol iso-propyl acetate iso-propylbenzene (cumene) iso-propyl ether methacrolein methacrylonitrile methanol 2-methoxyethanol (methyl cellosolve) methyl acetate methyl benzoate 2-methyl-1-butanol (active amyl alcohol) 2-methyl-2-butanol (tert-amyl alcohol) 3-methyl-1-butanol (iso-amyl alcohol) 3-methyl-2-butanone 2-methyl-3-buten-2-ol 3-methyl-2-buten-1-ol 2-methylbutyl acetate methylene chloride methyl formate 5-methyl-3-heptanone 5-methyl-2-hexanone 2-methyl-3-pentanone 4-methyl-2-pentanone (MIBK; methyl isobutyl ketone) 4-methyl-2-pentanol 4-methyl-3-penten-3-one -methylphenyl alcohol methyl propionate -methylstyrene 4-methylstyrene 1-methyl-2-pyrrolidone methyl tert-butyl ether (MTBE) morpholine nitrobenzene 2-nitrotoluene 3-nitrotoluene 4-nitrotoluene nonanal nonane 1,9-nonanediol 1-nonanol octanal octane 1,8-octanediol 1-octanol 2-octanone 3-octanone pentachlorobenzene pentadecane pentanal (valerlaldehyde) pentane 1,5-pentanediol 2,3-pentanedione 1-pentanol 2-pentanol 3-pentanol 2-pentanone 3-pentanone 1-penten-3-ol 2-penten-1-ol 1-penten-3-one (ethyl vinyl ketone) 3-penten-2-one (methyl vinyl ketone) pentyl ether 2-phenoxyethanol 4-phenyl-2-butanone (benzyl acetone) 1,3-propanediol 1-propanol 2-propen-1-ol (allyl alcohol) propionaldehyde propionic acid propionitrile propyl acetate propylbenzene propyl benzoate propylene glycol (1,2-propanediol) propyl ether propyl formate propyl propionate 2-propyn-1-ol (propargyl alcohol) pyridine sec-butanol sec-butyl acetate sec-butylbenzene styrene styrene oxide tert-amyl methyl ether tert-butanol Retention Time 5.40 13.41 2.47 15.13 22.08 13.80 17.73 8.58 15.75 5.83 4.45 19.77 19.73 20.13 9.06 21.25 20.03 18.19 4.85 13.80 7.84 17.06 2.13 14.09 3.26 20.77 15.29 14.27 13.55 10.10 5.03 11.44 12.39 11.30 Compound tert-butyl acetate tert-butylbenzene tert-butyl ethyl ether 4-tert-butyltoluene 1,2,4,5-tetrachlorobenzene 1,1,1,2-tetrachloroethane 1,1,2,2-tetrachloroethane tetrachloroethylene tetradecane tetrahydropyran HF (tetrahydrofuran) m-tolualdehyde o-tolualdehyde p-tolualdehyde toluene 1,2,3-trichlorobenzene 1,2,4-trichlorobenzene 1,3,5-trichlorobenzene 1,1,1-trichloroethane 1,1,2-trichloroethane trichloroethylene 1,2,3-trichloropropane 1,1,2-trichlorotrifluoroethane (Freon 113) tridecane triethylamine triglyme (triethylene glycol dimethyl ether) 1,2,3-trimethylbenzene (hemimellitene) 1,2,4-trimethylbenzene (pseudocumene) 1,3,5-trimethylbenzene (mesitylene) undecane vinyl acetate m-xylene o-xylene p-xylene

GC and GC/MS

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Pesticide Retention Data

Pesticide Elution Order Using Low Bleed Phases


Run Conditions: Columns: 30 m x 0.25 mm id, 0.25 m Carrier Gas: Helium at 35 cm/sec, measured at 50C Oven: 50C for 1 min 50-100C at 25/min 100-300C at 5/min (DB-1701 ramped to 280C) 300C for 5 min (DB-1701 held at 280C for 10 min)

Alphabetical Order By Analyte


Compound Alachlor Aldrin Aspon Atrazine Azinphos-ethyl Azinphos-methyl -BHC -BHC -BHC -BHC Bolstar 1-Bromo-2-nitrobenzene (IS) 2-Bromobiphenyl (SS) Captafol Captan Carbophenothion -Chlordane -Chlordane Chlorfenvinphos 4-Chloro-3-nitrobenzotrifluoride (SS) Chlorobenzilate Chloroneb Chloropropylate Chlorothalonil Chlorpyrifos Chlorpyrifos-methyl Coumaphos Crotoxyphos Dacthal p,p-DDD p,p-DDE p,p-DDT Demeton-O Demeton-S Diallate A Diallate B Diazinon 1,2-Dibromo-3-chloropropane DB-5ms 24.29 25.99 25.72 21.11 37.51 36.28 20.01 21.12 21.46 22.70 32.16 11.50 17.31 33.91 27.98 32.56 28.54 29.06 27.61 7.66 31.28 15.53 31.28 22.16 25.84 23.86 38.74 28.16 26.11 31.62 29.97 33.07 17.91 20.52 19.88 20.26 21.99 6.63 DB-XLB 25.84 27.33 26.69 22.65 39.94 38.83 21.83 24.95 23.37 25.98 33.89 12.73 18.49 36.35 30.15 34.49 30.72 30.90 29.34 8.55 32.82 16.87 32.92 26.44 27.52 25.64 41.40 29.48 27.55 33.93 31.82 35.12 18.97 21.83 20.87 21.35 23.05 7.11 DB-35ms 27.40 28.28 28.11 24.82 43.31 42.60 23.50 26.62 25.32 27.83 36.25 14.87 20.24 39.46 33.20 36.69 31.77 32.19 31.47 8.83 34.03 18.68 34.48 28.06 29.31 27.79 44.01 32.13 29.13 35.60 33.20 36.71 20.58 24.03 21.97 22.40 24.59 8.05 DB-17ms 27.91 28.54 27.55 24.50 43.27 42.75 23.83 26.70 25.73 28.08 35.94 15.66 21.01 40.31 34.14 36.26 31.91 32.43 31.15 8.59 34.27 19.37 34.85 28.08 28.86 27.55 43.52 32.09 29.61 35.92 33.53 37.05 20.13 23.67 22.31 22.71 24.21 8.47 31.89 28.82 34.60 31.50 35.37 20.49 24.05 21.42 22.10 24.16 7.90 DB-1701 27.59 26.79 28.22 25.12 48.74 45.86 23.06 24.84 27.97 28.89 34.96 14.68 18.62 40.40 32.25 35.48 30.91 31.21 31.02 10.00 33.78 17.92 33.98 27.73 28.36 26.70

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Pesticide Retention Data
Alphabetical Order By Analyte (Continued)
Compound ,-Dibromo-m-xylene Dibutylchlorendate (SS) Dichlofenthion Dichlorvos Dicrotophos Dieldrin Dimethoate Dioxathion Disulfoton Endosulfan I Endosulfan II Endosulfan sulfate Endrin Endrin aldehyde Endrin ketone EPN Ethion Ethoprop Ethylparathion Famphur DB-5ms 16.72 36.32 23.64 9.31 19.12 30.14 20.52 21.41 22.37 29.06 31.39 32.88 31.00 31.96 34.68 34.86 31.55 18.47 26.17 32.38 25.19 31.25 26.02 21.76 24.52 27.59 20.12 11.42 10.25 27.17 35.37 26.57 36.17 25.62 30.01 35.22 24.14 13.50 37.09 19.55 18.86 29.18 21.22 38.62 38.89 31.00 19.79 34.73 DB-XLB 18.27 37.75 25.07 9.93 20.58 32.03 22.32 22.78 23.68 30.95 34.11 35.74 32.92 34.57 37.26 37.04 33.09 19.60 28.09 34.38 26.96 33.31 27.62 23.19 25.98 29.32 22.13 11.94 11.10 28.71 37.59 28.71 38.15 26.96 31.47 37.05 26.14 14.48 39.12 21.15 20.15 31.15 23.47 40.27 40.57 32.67 21.02 37.24 DB-35ms 20.60 38.67 26.58 11.53 23.77 33.59 25.80 25.53 25.53 32.37 36.04 37.84 34.89 37.00 40.23 39.61 35.39 21.42 29.94 37.69 29.47 36.44 30.25 25.29 26.92 30.76 22.91 12.08 12.74 30.10 39.54 30.35 40.73 29.31 32.94 39.54 28.56 16.72 40.67 24.70 22.72 31.91 24.84 42.12 42.42 34.29 22.85 40.83 DB-17ms 21.40 38.74 26.02 11.39 23.98 33.90 26.02 25.49 25.09 32.61 36.36 38.13 35.35 37.52 40.99 39.26 35.11 21.02 29.36 37.72 29.22 36.36 30.22 25.03 27.11 31.07 23.01 12.25 12.54 30.48 39.91 30.68 40.55 29.13 32.22 40.31 28.22 16.69 40.99 24.97 22.70 31.91 25.08 42.53 42.80 34.68 22.45 40.91 40.94 29.20 31.89 38.91 28.57 17.56 37.96 26.50 22.41 31.29 23.64 42.25 46.52 32.51 22.33 42.38 DB-1701 19.41 39.65 26.02 12.45 24.66 32.16 26.70 24.80 24.95 30.72 34.97 38.91 32.97 37.01 41.57 41.19 34.85 21.29 30.10 39.69 29.36 37.10 29.07 24.41 25.69 29.68 21.03 11.60 15.46 28.44

GC and GC/MS

Fenitrothion Fensulfothion Fenthion Fonofos Heptachlor Heptachlor epoxide Hexachlorobenzene Hexachlorocyclopentadiene Hexamethylphosphoramide Isodrin Kelthane Kelthane Decomp. Product Leptophos Malathion Merphos Methoxychlor Methylparathion Mevinphos Mirex Monocrotophos Naled trans-Nonachlor Pentachloronitrobenzene (IS) cis-Permethrine trans-Permethrine Perthane Phorate Phosmet

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Pesticide Retention Data/Environmental-Hydrocarbons
Alphabetical Order By Analyte (Continued)
Compound Phosphamidon Propachlor Ronnel Simazine Stirophos Sulfotep TEPP Terbufos Terrazole Tetrachloro-m-xylene (SS) Thionazin Tokuthion Trichloronate Trifluralin Tri-o-cresylphosphate DB-5ms 23.56 17.88 24.58 20.91 28.66 19.37 16.76 21.64 14.17 18.12 17.72 29.55 26.51 19.30 36.64 DB-XLB 23.40 19.32 26.14 22.65 30.50 20.42 17.91 22.78 15.12 19.81 18.97 31.19 28.09 20.35 38.40 DB-35ms 27.79 21.17 27.95 25.05 32.94 22.46 20.69 24.23 16.60 20.24 21.26 32.83 29.31 19.98 40.93 DB-17ms 27.72 21.81 27.55 24.87 32.69 22.27 20.74 23.67 17.25 20.32 21.12 32.22 28.59 19.47 40.83 DB-1701 28.85 21.74 27.10 25.23 32.27 22.56 21.98 23.91 15.86 18.70 20.85 31.67 28.57 22.15 42.62

Methyl tert-butyl ether (MTBE) PID, Extended 8020 Analysis


Column:
P/N: Carrier: Oven:

Methyl tert-butyl ether (MTBE) FID, Extended 8020 Analysis


Column:
15. 1,3,5-Trimethylbenzene 16. 1,2,4-Trimethylbenzene 17. 1,2,3-Trimethylbenzene 18. Naphthalene SS - Surrogate Standard P/N: Carrier: Oven:

DB-MTBE
30 m x 0.45 mm id, 2.55 m 124-0034 Helium at 10 mL/min 35C for 4 min 35-200C at 20/min 200C for 5 min Purge and trap (O.I.A. 4560) J&W LVI (Low Volume Injector), 150C 40 ppb per component in 5 mL water Tenax only 175C 180C for 3 min FID (O.I.A. 4410), 200C 15. 1,3,5-Trimethylbenzene 16. 1,2,4-Trimethylbenzene 17. 1,2,3-Trimethylbenzene 18. Naphthalene SS - Surrogate Standard

GC and GC/MS

DB-MTBE
30 m x 0.45 mm id, 2.55 m 124-0034 Helium at 10 mL/min 35C for 4 min 35-200C at 20/min 200C for 5 min Purge and trap (O.I.A. 4560) J&W LVI (Low Volume Injector), 150C 40 ppb per component in 5 mL water Tenax only 175C 180C for 3 min PID (O.I.A. 4430), 200C

Injector:

Injector:

Trap: Preheat: Desorb: Detector:

Trap: Preheat: Desorb: Detector:

1. Methyl-tert-butyl ether (MTBE) 2. 2-Methylpentane 3. 3-Methylpentane 4. Diisopropyl ether (DIPE) 5. Ethyl-tert-butyl ether (ETBE) 6. Benzene 7. tert-Amyl methyl ether (TAME) 8. ,,-Trifluorotoluene (SS) 9. Toluene 10. Ethylbenzene 11. m-Xylene 12. p-Xylene 13. o-Xylene 14. Cumene

1. Methyl-tert-butyl ether (MTBE) 2. 2-Methylpentane 3. 3-Methylpentane 4. Diisopropyl ether (DIPE) 5. Ethyl-tert-butyl ether (ETBE) 6. Benzene 7. tert-Amyl methyl ether (TAME) 8. ,,-Trifluorotoluene (SS) 9. Toluene 10. Ethylbenzene 11. m-Xylene 12. p-Xylene 13. o-Xylene 14. Cumene

C539

C540

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347

GC and GC/MS Applications


Environmental-Hydrocarbons
Unleaded Gasoline
Column:
P/N: Carrier: Oven:

DB-VRX
30 m x 0.45 mm id, 2.55 m 124-1534 Helium at 109 cm/sec (10.4 mL/min), measured at 40C 40C for 2 min 40-200C at 12/min 200C for 5 min Purge and trap (O.I.A. 4460A) J&W LVI (Low Volume Injector) 115 ppb gasoline in 5 mL water BTEX (Supelco) at 50C during purge 1 min at 270C PID (O.I.A. 4430), 200C FID, 250C

Injector:

Trap: Desorb: Detector A: Detector B:

1. 3-Methylpentane 2. Benzene 3. Isooctane 4. Toluene 5. Ethylbenzene 6. m,p-Xylene 7. o-Xylene 8. 1,2,4-Trimethylbenzene 9. Naphthalene

FID

PID

GC and GC/MS

C174

Diesel Fuel
Column:
P/N: Carrier: Oven:

DB-5ms
30 m x 0.53 mm id, 1.5 m 125-5532 Helium at 48.5 cm/sec, measured at 60C 60C for 2 min 60-300C at 12/min 300C for 10 min Megabore Direct, 280C 1 L injection in hexane FID, 250C Nitrogen makeup gas at 30 mL/min 1. n-C10, Decane 2. n-C12, Dodecane 3. n-C14, Tetradecane 4. n-C16, Hexadecane 5. n-C18, Octadecane 6. n-C20, Eicosane 7. n-C22, Docosane 8. n-C24, Tetracosane 9. n-C26, Hexacosane 10. n-C28, Octacosane

Injector: Detector:

Diesel Fuel Standard 50 ng/component

Diesel Fuel 0.6 mg/mL

C175

348

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GC and GC/MS Applications


Environmental-Pesticides and Herbicides
CLP Pesticides
Column:
P/N: 1. Tetrachloro-m-xylene (SS) 2. -BHC 3. -BHC 4. -BHC 5. Heptachlor 6. -BHC 7. Aldrin 8. Heptachlor epoxide 9. -Chlordane 10. -Chlordane 11. Endosulfan I 12. 4,4-DDE 13. Dieldrin 14. Endrin 15. 4,4-DDD 16. Endosulfan II 17. 4,4-DDT 18. Endrin aldehyde 19. Endosulfan sulfate 20. Methoxychlor 21. Endrin ketone 22. Decachlorobiphenyl (SS) SS - Surrogate Standard 12 8 1 5 6 18 4 14 17 19 22 9 10 11 15 16 21 13

DB-35ms
30 m x 0.32 mm id, 0.25 m film 123-3832

Column:
P/N: Carrier: Oven:

DB-XLB
30 m x 0.32 mm id, 0.5 m film 123-1236 Helium at 45 cm/sec (EPC in constant flow mode) 110C for 0.5 min 110-320C at 15 C/min 320C for 2 min Splitless, 250C 30 sec purge activation time 50 pg per component ECD, 350C Nitrogen makeup gas (column + makeup flow = 30 mL/min constant flow) 2

Injector:

Detector:

DB-35ms
3 7

GC and GC/MS

20

C784-2

10 Time (min)

12

14

16

DB-XLB
2

Complete resolution and confirmation of 22 CLP Pesticides in under 16 minutes!


3 7 8 9 10 11 15 6 5 4 17 14 18 19 22 12 13

16 21

20

10 Time (min)

12

14

16

C783

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349

GC and GC/MS Applications


Environmental-Pesticides and Herbicides
Organochlorine Pesticides (EPA Method 8081A)
Column:
P/N: Carrier: Oven: 9. Tetrachloro-m-xylene (SS) 10. , -Dibromo-m-xylene 11. Propachlor 12. Di-allate A 13. Di-allate B 14. Hexachlorobenzene 15. -BHC 16. Pentachloronitrobenzene (IS) 17. -BHC 18. -BHC 19. Heptachlor 20. Alachlor 21. -BHC 22. Chlorothalonil 23. Aldrin 24. Dacthal 25. Isodrin 26. Kelthane 27. Heptachlor epoxide 28. -Chlordane 29. trans-Nonachlor 30. -Chlordane 31. Endosulfan I 32. Captan 33. p,p-DDE 34. Dieldrin 35. Chlorobenzilate 36. Perthane 37. Chloropropylate 38. Endrin 39. p,p-DDD 40. Endosulfan II 41. p,p-DDT 42. Endrin aldehyde 43. Endosulfan sulfate 44. Dibutylchlorendate (SS) 45. Captafol 46. Methoxychlor 47. Endrin ketone 48. Mirex 49. cis-Permethrin 50. trans-Permethrin * Breakdown Products SS - Surrogate Standard IS - Internal Standard Standards used were a composite of individual solutions supplied courtesy of Accustandard Inc., 25 Science Park, New Haven, CT 06511, 800-442-5290.

DB-35ms
30 m x 0.25 mm id, 0.25 m 122-3832 Helium at 35 cm/sec, measured at 50C 50C for 1 min 50-100C at 25/min 100-300C at 5/min 300C for 5 min Splitless, 250C 30 sec purge activation time 1 L of 35 g/mL composite 8081A standards, Accustandard Inc. MSD, 300C transfer line full scan at m/z 50-500

Injector:

Detector:

1. 1,2-Dibromo-3-chloropropane 2. 4-Chloro-3-nitrobenzotrifluoride (SS) 3. Hexachloropentadiene 4. 1-Bromo-2-nitrobenzene (IS) 5. Terrazole 6. Chloroneb 7. Trifluralin 8. 2-Bromobiphenyl (SS)

GC and GC/MS

C512

Organochlorine Pesticides (EPA Method 8081A)


Column:
P/N: Carrier: Oven:

DB-5ms
30 m x 0.25 mm id, 0.25 m 122-5532 Helium at 35 cm/sec, measured at 50C 50C for 1 min 50-100C at 25/min 100-300C at 5/min 300C for 5 min Splitless, 250C 30 sec purge activation time 1 L of 35 g/mL composite 8081A standards, Accustandard Inc. MSD, full scan at m/z 50-500 300C transfer line

Injector:

Detector:

1. 1,2-Dibromo-3-chloropropane 2. 4-Chloro-3-nitrobenzotrifluoride (SS) 3. Hexachloropentadiene 4. 1-Bromo-2-nitrobenzene (IS) 5. Terrazole 6. Chloroneb 7. Trifluralin 8. 2-Bromobiphenyl (SS) 9. Tetrachloro-m-xylene (SS) 10. , Dibromo-m-xylene 11. Propachlor 12. Di-allate A 13. Di-allate B 14. Hexachlorobenzene 15. -BHC 16. Pentachloronitrobenzene (IS) 17. -BHC 18. -BHC 19. Heptachlor 20. Alachlor 21. -BHC

22. Chlorothalonil 23. Aldrin 24. Dacthal 25. Isodrin 26. Kelthane 27. Heptachlor epoxide 28. -Chlordane 29. trans-Nonachlor 30. Chlordane 31. Endosulfan I 32. Captan 33. p, p-DDE 34. Dieldrin 35. Chlorobenzilate 36. Perthane 37. Chloropropylate

38. Endrin 39. p, p-DDD 40. Endosulfan II 41. p, p-DDT 42. Endrin aldehyde 43. Endosulfan sulfate 44. Dibutylchlorendate (SS) 45. Captafol 46. Methoxychlor 47. Endrin ketone 48. Mirex 49. cis-Permethrin 50. trans-Permethrin * Breakdown Products SS - Surrogate Standard IS - Internal Standard

C494

350

www.agilent.com/chem

GC and GC/MS Applications


Environmental-Pesticides and Herbicides
EPA 508.1 Pesticides
Column:
P/N: 1. Hexachlorocyclopentadiene 2. Etridiazole 3. Chloroneb 4. Trifluralin 5. Propachlor 6. Hexachlorobenzene 7. -BHC 8. Atrazine 9. Pentachloronitrobenzene 10. Simazine 11. -BHC 12. -BHC 13. Heptachlor 14. Alachlor 15. -BHC 16. Chlorothalonil 17. Aldrin 18. Metribuzin 19. Metolachlor 20. DCPA
28 24 25 26 13 17 32 18 1 12 2 9,10 4 30 31 33 35

DB-35ms
30 m x 0.32 mm id, 0.25 m 123-3832

Column:
P/N: Carrier: Oven:

DB-XLB
30 m x 0.32 mm id, 0.50 m 123-1236 Helium at 45 cm/sec (EPC in constant flow mode) 75C for 0.5 min 75-300C at 10 C/min 300C for 2 min Splitless, 250C 30 sec purge activation time 50 pg per component 7 ECD, 350C Nitrogen makeup gas (column + makeup flow = 30 mL/ 6 min constant flow)
11

Injector:

Detector:

16 15

21. 4,4-Dibromobiphenyl 22. Heptachlor epoxide 23. Cyanazine 24. Chlordane 25. -Chlordane 26. Endosulfan I 27. 4,4-DDE 28. Dieldrin 29. Chlorobenzilate 30. Endrin 31. 4,4-DDD 32. Endosulfan II 33. 4,4-DDT 34. Endrin aldehyde 35. Endosulfan sulfate 36. Methoxychlor 37. cis-Permethrin 38. trans-Permethrin

21,22

DB-35ms
20

27

36 23 3 14 5 8 19 29 37 34 38

GC and GC/MS

C786

10

12

14

16 Time (min) 7 6

18

20

22

24

DB-XLB

8,11

15 16 24 20 13 17 22 26 32 35 33 28 27

25

1 4 2

18 12

30 31

21 23 3 5 8,10 14 19 29 34

36

38 37

10

12

14

C787

16 Time (min)

18

20

22

24

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351

GC and GC/MS Applications


Environmental-Pesticides and Herbicides
Chlorinated Pesticides: EPA Method 508
Column:
P/N: GC: Carrier: Oven:

HP-5ms
30 m x 0.25 mm id, 0.25 m 19091S-433 5890 EPC Helium, 24 psi, 45 cm/sec (80C) constant flow 80C for 1 min 80-180C at 30C/min 180-205C at 3C/min 205C for 4 min 205-290C at 20C/min 290C for 2 min Splitless, 1 L, 1 min purge delay ECD 320C, Nitrogen, 60 mL/min makeup gas 3 mL/min anode purge 6 5 10 13 8 9 15 17 1. Etridiazole 2. Chloroneb 3. Propachlor 4. Trifluralin 5. -BHC 6. Hexachlorobenzene 7. -BHC 8. -BHC 9. -BHC 10. Chlorothalonil 11. Heptachlor 12. Aldrin 13. DCPA 14. Heptachlor epoxide 15. -Chlordane 16. Endosulfan I 17. -Chlordane 18. Dieldrin 19. 4,4'-DDE 20. Endrin 21. Endosulfan II 22. Chlorobenzilate 23. 4,4'-DDD 24. Endrin aldehyde 25. Endosulfan sulfate 26. 4,4'-DDT 27. Methoxychlor 28. cis-Permethrin 29. trans-Permethrin 29

Injection: Detector:

4 1

12 7 11 25 14 16 18 19 27 21 20 26 28

2 3

23 24 22

GC and GC/MS

C1023

10 Time (min)

15

20

Organohalide Pesticides in Water, EPA Method 505


Column: HP-5MS
P/N: Carrier: Oven: 30 m x 0.25 mm id, 0.25 m 19091S-433 Helium, 2.0 m./min, constant flow, 42 cm/sec (22.4 psi at 80C) 1. Hexachlorocyclopentadiene 9. Heptachlor epoxide 80C for 1 min 2. Hexachlorobenzene 10. -Chlordane 80-175C at 30C/min 3. Simazine 11. -Chlordane 175C for 4 min 4. Atrazine 12. trans-Nonachlor 175- 215C at 6C/min 5. Lindane 13. Dieldrin 215C for 2 min 6. Heptachlor 14. Endrin 215- 290Cat 15C/min 7. Alachlor 15. cis-Nonachlor 290C for 5 min 8. Adrin 16. Methoxychlor Splitless, 250C, 1 min purge delay 1 L injection volume Sample: ECD, 300C 16 components EPA-505 targeted pesticides and 14 ppb Makeup gas: N2, 60 mL/min Aroclor 1260 in hexane. Anode purge 6 mL/min Concentration of pesticides: 50 ppb each except 1.25 ppm for atrazine and simazine. 2 4 3 5 8 7 6 1 9 11 10 12 14 13 15 16

Injector:

Detector:

C1038

10

15 Time (min)

20

352

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GC and GC/MS Applications


Environmental-Pesticides and Herbicides
Organochlorine Pesticides
Column:
P/N: Carrier: Oven: Injector: Detector:

DB-5
30 m x 0.53 mm id, 0.5 m 125-5037 Helium at 30 cm/sec (4.0 mL/min) 150-275C at 4/min 275C for 30 min Megabore Direct, 250C 0.7 L of 100 pg/L standard in isooctane ECD, 300C Nitrogen makeup gas at 30 mL/min

1. 2,4,5,6-Tetrachloro-m-xylene (IS) 2. -BHC 3. -BHC 4. -BHC 5. -BHC 6. Heptachlor 7. Aldrin 8. Heptachlor epoxide 9. -Chlordane 10. Endosulfan I 11. -Chlordane 12. Dieldrin 13. p,p-DDE 14. Endrin 15. Endosulfan II 16. p,p-DDD 17. Endrin aldehyde 18. Endosulfan sulfate 19. p,p-DDT 20. Endrin ketone 21. Methoxychlor 22. Decachlorobiphenyl (IS)

GC and GC/MS

C177

Organochlorine Pesticides
Column:
P/N: Carrier: Oven: Injector: Detector:

DB-608
30 m x 0.53 mm id, 0.5 m 125-6837 Helium at 30 cm/sec (4.0 mL/min) 150-275C at 4/min 275C for 30 min Megabore Direct, 250C 0.7 L of 100 pg/L standard in isooctane ECD, 300C Nitrogen makeup gas at 30 mL/min

1. 2,4,5,6-Tetrachloro-m-xylene (IS) 2. -BHC 3. -BHC 4. -BHC 5. -BHC 6. Heptachlor 7. Aldrin 8. Heptachlor epoxide 9. -Chlordane 10. Endosulfan I 11. -Chlordane 12. Dieldrin 13. p,p-DDE 14. Endrin 15. Endosulfan II 16. p,p-DDD 17. Endrin aldehyde 18. Endosulfan sulfate 19. p,p-DDT 20. Endrin ketone 21. Methoxychlor 22. Decachlorobiphenyl (IS)

C178

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353

GC and GC/MS Applications


Environmental-Pesticides and Herbicides
Organochlorine Pesticides
Column:
P/N: Carrier: Oven: Injector: Detector:

DB-1701
30 m x 0.53 mm id, 0.5 m 125-0737 Helium at 30 cm/sec (4.0 mL/min) 150-275C at 4/min 275C for 30 min Megabore Direct, 250C 0.7 L of 100 pg/L standard in isooctane ECD, 300C Nitrogen makeup gas at 30 mL/min

1. 2,4,5,6-Tetrachloro-m-xylene (IS) 2. -BHC 3. -BHC 4. -BHC 5. -BHC 6. Heptachlor 7. Aldrin 8. Heptachlor epoxide 9. -Chlordane 10. Endosulfan I 11. -Chlordane 12. Dieldrin 13. p,p-DDE 14. Endrin 15. Endosulfan II 16. p,p-DDD 17. Endrin aldehyde 18. Endosulfan sulfate 19. p,p-DDT 20. Endrin ketone 21. Methoxychlor 22. Decachlorobiphenyl (IS)

GC and GC/MS

C180

Organochlorine Pesticides
Column:
P/N: Carrier: Oven: Injector: Detector:

DB-35
30 m x 0.53 mm id, 0.5 m 125-1937 Helium at 30 cm/sec (4.0 mL/min) 150-275C at 4/min 275C for 30 min Megabore Direct, 250C 0.7 L of 100 pg/L standard in isooctane ECD, 300C Nitrogen makeup gas at 30 mL/min

1. 2,4,5,6-Tetrachloro-m-xylene (IS) 2. -BHC 3. -BHC 4. -BHC 5. -BHC 6. Heptachlor 7. Aldrin 8. Heptachlor epoxide 9. -Chlordane 10. Endosulfan I 11. -Chlordane 12. Dieldrin 13. p,p-DDE 14. Endrin 15. Endosulfan II 16. p,p-DDD 17. Endrin aldehyde 18. Endosulfan sulfate 19. p,p-DDT 20. Endrin ketone 21. Methoxychlor 22. Decachlorobiphenyl (IS)

C179

354

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GC and GC/MS Applications


Environmental-Pesticides and Herbicides
Organochlorine Pesticides, DB-5/1701P Dual Column
Columns: DB-5
30 m x 0.32 mm id, 0.25 m

DB-1701P
Guard Column: P/N: Carrier: Oven: 30 m x 0.32 mm id, 0.21 m 10 m x 0.53 mm id 3-way union 009-5007 Helium at 29.2 cm/sec, measured at 150C 60C for 0.5 min 60-140C at 20/min 140-280C at 11/min 280C for 23 min Splitless, 200C 2.0 L, 20-200 pg/L ECD, 325 Nitrogen makeup gas at 30 mL/min

Injector: Detector:

1. Etridiazole 2. Chloroneb 3. Propachlor 4. Tetrachloro-m-xylene (IS) 5. Trifluralin 6. -BHC 7. Hexachlorobenzene 8. -BHC 9. -BHC 10. Pentachloronitrobenzene 11. p,p'-Dichlorobiphenyl 12. -BHC 13. Heptachlor 14. Alachlor 15. Aldrin

16. Chlorpyrifos 17. DCPA 18. Isodrin 19. Heptachlor epoxide 20. Captan 21. -Chlordane 22. o,p-DDE 23. Endosulfan I 24. -Chlordane 25. Dieldrin 26. p,p-DDE 27. o,p-DDD 28. Endrin 29. Endosulfan II 30. Chlorobenzilate

31. p,p-DDD 32. o,p-DDT 33. Endrin aldehyde 34. Endrin ketone 35. Carbophenothion 36. p,p-DDT 37. Endosulfan sulfate 38. Hexabromobenzene (HBB) 39. Methoxychlor 40. Mirex 41. cis-Permethrin 42. trans-Permethrin 43. Decachlorobiphenyl (IS)

GC and GC/MS

Enlarged view of critical pair on DB-5

DB-5 DB-1701

Enlarged view of critical pairs on DB-1701 C176

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355

GC and GC/MS Applications


Environmental-Pesticides and Herbicides
Nitrogen/Phosphorus Containing Pesticides: EPA Method 507
Columns:
P/N: GC: Carrier: Oven:

HP-5MS
30 m x 0.25 mm id, 0.25 m 19091S-433 5890 Series II Helium, 30 cm/sec (13.6 psi) pressure program 80-178C at 30C/min 178C for 4 min 178-205C at 2C/min 205-310C at 30C/min 310C for 4 min Splitless mode, 260C 1 min purge delay NPD, 290C Helium makeup gas at 30 mL/min

Injector: Detector:

1. Dichlorvos 2. EPTC 3. Butylate 4. Mevinphos 5. Vernolate 6. Pebulate 7. Tebuthiuron 8. Molinate 9. Ethoprop 10. Cycloate 11. Chlorpropham 12. Atraton 13. Simazine 14. Prometon 15. Atrazine

16. Propazine 17. Terbufos 18. Pronamide 19. Diazinon 20. Disulfoton 21. Terbacil 22. Metribuzin 23. Simetryn 24. Alachlor 25. Ametryn 26. Prometryn 27. Terbutryn 28. Bromacil 29. Metolachlor 30. Triademefon
41

31. MGK-264 32. Diphenamid 33. Stirofos 34. Butachlor 35. Fenamiphos 36. Napropamide 37. Tricyclazole 38. Merphos 39. Carboxin 40. Norflurazon 41. Hexazinone 42. Fenarimol 43. Fluridone

9 2 5 36 7 8

14 13 15 12 16

23 24 25,26

10

17 20 19 22

27 38 30

40

42

1 4 11 18 21 28

32 29 31

37 36 35 33 34 39

43

GC and GC/MS

C1025

10

15 Time (min)

20

25

Organophosphorous Pesticides (EPA Method 8141A)


Column:
P/N: Carrier: Oven:

DB-5ms
30 m x 0.25 mm id, 0.25 m 122-5532 Helium at 35 cm/sec, measured at 50C 50C for 1 min 50-100C at 25/min 100-300C at 5/min 300C for 5 min Splitless, 250C 2 30 sec purge activation time 1 L of 40 g/mL 8141A standards, Accustandard Inc. MSD, 300C transfer line full scan at m/z 50-500

Injector:

Detector:

Note: All standards used were supplied courtesy of Accustandard Inc., 25 Science Park, New Haven, CT 06511, 800-442-5290.

1 3

1. 4-Chloro-3-nitrotrifluoride 20. Fonofos 30. Aspon 2. Dichlorovos 21. Diazinon 31. Chlorpyrifos 3. Hexamethylphosphoramide 22. Disulfoton 32. Fenthion 4. Mevinphos 23. Phosphamidon 33. Ethyl parathion 5. TEPP 24. Dichlofenthion 34. Trichloronate 6. Thionazin 25. Chlorpyrifos-methyl 35. Chlorfenvinphos 7. Demeton-O 26. Methyl parathion 36. Crotoxyphos 8. Ethoprop 27. Ronnel 37. Stirophos 9. Naled 28. Fenitrothion 38. Tokuthion 10. Dicrotophos 29. Malathion 39. Merphos 11. Sulfotepp 40. Fensulfothion 21 12. Monocrotophos 41. Ethion 13. Phorate 42. Bolstar 22 14. Dimethoate 31 8 13 15. Demeton-S 20 16. Simazine 34 38 19 17. Atrazine 27 32 11 18. Dioxathion 30 7 24 19. Terbufos 6 36 39 37 29 4 17 25 26 35 14,15 23 33 12 16 28
9 18

43. Famphur 44. Carbophenothion 45. Phosmet 46. EPN 47. Leptophos 48. Azinphos methyl 49. Tri-o-cresyl phosphate 50. Azinphos ethyl 51. Coumaphos * Breakdown products

41

42 44 43 49 45 50 48 47 51

40

46

* 5 10 * * * *

10
C516

15

20 Time (min)

25

30

35

356

www.agilent.com/chem

GC and GC/MS Applications


Environmental-Pesticides and Herbicides
Organophosphorous Pesticides (EPA Method 8141A)
Column:
P/N: Carrier: Oven: 1. 4-Chloro-3-nitrotrifluoride 2. Dichlorvos 3. Hexamethylphosphoramide 4. Mevinphos 5. TEPP 6. Thionazin 7. Demeton-O 8. Ethoprop 9. Naled 10. Dicrotophos 11. Sulfotepp 12. Monocrotophos 13. Phorate 14. Dimethoate 15. Demeton-S 16. Simazine 17. Atrazine 18. Dioxathion 19. Terbufos 20. Fonofos 21. Diazinon 22. Disulfoton 23. Phosphamidon 24. Dichlofenthion 25. Chlorpyrifos-methyl 26. Methyl parathion 27. Ronnel 28. Fenitrothion 29. Malathion 30. Aspon 31. Chlorpyrifos 32. Fenthion 33. Ethyl parathion 34. Trichloronate 35. Chlorfenvinphos 36. Crotoxyphos 37. Stirophos 38. Tokuthion 39. Merphos 40. Fensulfothion 41. Ethion 42. Bolstar 43. Famphur 44. Carbophenothion 45. Phosmet 46. EPN 47. Leptophos 48. Azinphos methyl 49. Tri-o-cresyl phosphate 50. Azinphos ethyl 51. Coumaphos * Breakdown products

DB-35ms
30 m x 0.25 mm id, 0.25 m 122-3832 Helium at 35 cm/sec, measured at 50C 50C for 1 min 50-100C at 25/min 100-300C at 5/min 300C for 5 min Splitless, 250C 30 sec purge activation time 1 L of 40 g/mL 8141A standards, Accustandard Inc. MSD, full scan at m/z 50-500 300C transfer lin

Injector:

Detector:

Note: All standards used were supplied courtesy of Accustandard Inc., New Haven, CT

GC and GC/MS

C517

EPA 8151A Phenoxy Acid Herbicides - Methyl Derivatives


Column:
P/N:

DB-35ms
30m x 0.32 mm id, 0.25 m 123-3832

Column:
P/N: Carrier: Oven:

DB-XLB
30m x 0.32 mm id, 0.50 m 123-1236 Helium at 45 cm/sec (EPC in constant flow mode) 50C for 0.5 min 50-100C at 25C/min 100-320C at 12C/min 320C for 2 min Splitless, 250C 30 sec purge activation time 50 pg per component ECD, 350C Nitrogen makeup gas (column + makeup flow = 30 mL/min constant flow)
1

1. Dalapon 2. 3,5-Dichlorobenzoic acid 3. 4-Nitrophenol 4. Methyl-2,4-dichlorophenylacetate (SS) 5. Dicamba 6. MCPP 7. MCPA 8. 4,4-Dibromooctafluorobiphenyl (IS) 9. Dichloroprop 10. 2,4-D

11

11. Pentachlorophenol 12. 2,4,5-T,P 13. 2,4,5-T 14. Chloramben 15. Dinoseb 16. 2,4-DB 17. Bentazone 18. DCPA 19. Picloram 20. Acifluorofen

Injector:

Detector:

12 5 2 3 10

18 13 14 15 19 20

DB-35ms
4

16

17

8 Time (min)

10

12 11

14

16

DB-XLB
12 5 1 2 3 4 10 19 14 13 7 9 15 16 18 20

17

8 Time (min)

10

12

14

16

C785

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357

GC and GC/MS Applications


Environmental-Pesticides and Herbicides
Phenoxy Acid Herbicides
Column:
P/N: Carrier: Oven:

DB-1701
30 m x 0.32 mm id, 0.25 m 123-0732 Helium at 35 cm/sec, measured at 50C 50C for 1 min 50-160C at 10/min 160C for 3 min 160-260C at 10/min 260C for 5 min Splitless, 250C 45 sec purge activation time 1 L of 0.1 g/L standard in methanol FID, 300C Nitrogen makeup gas at 30 mL/min

TMS Esters

Injector:

Detector:

1. Dalapon 2. Dicamba 3. MCPP 4. MCPA 5. Dichloroprop 6. 2,4-D 7. Pentachlorophenol 8. Dinoseb 9. 2,4,5-TP 10. 2,4,5-T 11. 2,4-DB 12. Picloram

Methyl Esters

GC and GC/MS

C014

Herbicides
Column:
P/N: Carrier: Oven:

DB-XLB
30 m x 0.25 mm id, 0.25 m 122-1232 Helium at 32 cm/sec, measured at 50C 50C for 1 min 50-180C at 10/min 180-230C at 5/min 230-320C at 10/min 320C for 2 min Splitless, 250C 30 sec purge activation time 2 L x 10-50 ng/L solution in acetone MSD, 50-400 full scan transfer line 300C

Injector:

1. Monuron 2. Diuron 3. EPTC 4. Dichlobenil 5. Vernolate 6. Pebulate 7. Molinate 8. Sulfallate 9. Atraton 10. Prometon 11. Atrazine 12. Propazine

13. Simazine 14. Terbuthylazine 15. Pronamide 16. Secbumeton 17. Terbacil 18. Alachlor 19. Propanil 20. Ametryne 21. Prometryne 22. Simetryn 23. Metribuzin 24. Terbutryn

25. Metolachlor 26. Bromacil 27. Dacthal 28. Diphenamid 29. Butachlor 30. Napropamide 31. Carboxin 32. Tricyclazole 33. Norflurazon 34. Hexazinone 35. Difolotan 36. Fluridone *Impurity

Detector:

C414

358

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GC and GC/MS Applications


Environmental-Pesticides and Herbicides/Environmental-Semivolatiles
Triazine Herbicides
Column:
P/N: Carrier: Oven:

Triazine Herbicides
Column:
P/N: Carrier: Oven:

DB-1701
30 m x 0.32 mm id, 0.25 m 123-0732 Helium at 35 cm/sec, measured at 50C 50C for 1 min 50-100C at 25/min 100-250C at 5/min Splitless, 250C, 40 sec purge activation time 1 L of 0.1 g/L in methanol FID, 300C Nitrogen makeup gas at 30 mL/min 1. 2-Nitroxylene 2. Atraton 3. Prometon 4. Propazine 5. Atrazine 6. Simazine 7. Terbuthylazine 8. Secbumeton 9. Ametryne 10. Prometryne 11. Simetryne 12. Terbutryne

DB-17
30 m x 0.25 mm id, 0.25 m 122-1732 Helium at 35 cm/sec, measured at 50C 50C for 1 min 50-100C at 25/min 100-250C at 5/min Splitless, 250C 40 sec purge activation time 1 L of 0.1 g/L in methanol FID, 300C Nitrogen makeup gas at 30 mL/min 1. 2-Nitroxylene 2. Atraton 3. Propazine 4. Prometon 5. Atrazine 6. Simazine 7. Terbuthylazine 8. Secbumeton 9. Prometryne 10. Ametryne 11. Simetryne 12. Terbutryne

Injector: Detector:

Injector:

Detector:

GC and GC/MS

C184

C183

Tetrachlorodibenzo-p-furans
Note the separation between 2,3,7,8-TCDF and 2,3,4,7-TCDF on DB-225 is also easily achievable (actually a little better!) on DB-225ms.

Column:
P/N: Carrier: Oven: Injector: Detector:

DB-225
30 m x 0.25 mm id, 0.25 m 122-2232 Helium at 12 mL/min 160-250C at 7/min 250C until compounds elute Splitless, 240C VG Autospec Ultima

Column:
P/N: Carrier: Oven: Injector: Detector:

DB-225ms
30 m x 0.25 mm id, 0.25 m 122-2932 Helium at 12 mL/min 160-250C at 7/min 250C until compounds elute Splitless, 240C VG Autospec Ultima

C500

C501

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359

GC and GC/MS Applications


Environmental-Semivolatiles
Dioxins and Furans
Column:
P/N: Carrier: Oven:

Tetra Isomers

Penta Isomers

DB-Dioxin
60 m x 0.25 mm id, 0.15 m 122-2461 Helium at 34.3 cm/sec, measured at 250C 180C for 1 min 180-270C at 2.5/min 270C for 40 min

GC and GC/MS

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33.

1,3,6,8-TCDD 2,3,7,8-TCDD 1,2,8,9-TCDD 1,2,4,6,8-/1,2,4,7,9-PeCDD 1,2,3,7,8 + unknown-PeCDD 1,2,4,8,9-PeCDD 1,2,4,6,7,9-/1,2,4,6,8,9-HeCDD 1,2,3,4,7,8-HeCDD 1,2,3,4,6,9-HeCDD 1,2,3,6,7,8-HeCDD 1,2,3,7,8,9-HeCDD 1,2,3,4,6,7-HeCDD 1,2,3,4,6,7,9-HpCDD 1,2,3,4,6,7,8-HpCDD OcCDD 1,3,6,8-TCDF 2,3,7,8-TCDF 1,2,8,9-TCDF 1,3,4,6,8-PeCDF 1,2,3,4,8-PeCDF 1,2,3,7,8-PeCDF 1,2,3,4,6-PeCDF 2,3,4,7,8-PeCDF 1,2,3,8,9-PeCDF 1,3,4,6,7,9-HeCDF 1,2,3,4,7,8/1,2,4,6,8,9-HeCDF 1,2,3,6,7,8-HeCDF 2,3,4,6,7,8-HeCDF 1,2,3,7,8,9-HeCDF 1,2,3,4,8,9-HeCDF 1,2,3,4,6,7,8-HpCDF 1,2,3,4,7,8,9-HpCDF OcCDF

C041A

Hexa Isomers

Hepta Isomers

Octa Isomers

C041A

C041b

360

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GC and GC/MS Applications


Environmental-Semivolatiles
Aroclors 1016-1268 (without 1221)
Column:
P/N: Carrier: Oven: Injector: Detector:

DB-XLB
30.0 m x 0.18 mm id, 0.18 m 121-1232 Helium at 37 cm/sec, measured at 150C 100C for 1 min 100-265C at 1.2/min Hot On-column, 250C 1 L in isooctane, 12.5 ppm MSD, SIM, 340C transfer line

C549

IUPAC 3 2 1 4 10 9 7 6 5 8 19 14 30 11 18 17 12 13 27 24 16 15 32 54 34 23 29 50 26 25 31 53 28 21 33 20 51 45 22 46 73 36

Retention Time 26.03 32.69 33.92 35.61 35.74 40.70 40.85 42.15 43.11 43.64 45.84 46.58 48.01 50.08 50.25 50.60 50.85 51.69 51.74 52.08 52.95 53.46 53.70 54.87 55.33 55.54 56.14 57.51 57.60 57.98 59.42 59.43 59.79 59.84 60.05 60.10 60.36 61.48 61.87 62.44 63.60 63.97

IUPAC 69 43 52 48 49 104 39 65 47 62 38 75 44 59 42 35 71 41 96 64 40 37 72 103 68 100 67 94 57 58 102 61 98 93 76 63 88 74 70 121 95 91

Retention Time 64.03 64.08 64.57 64.85 65.24 65.62 65.80 65.85 65.86 65.87 65.96 66.23 67.36 67.95 67.98 68.52 68.89 68.93 69.31 70.13 70.22 70.33 70.34 70.74 70.90 71.75 71.99 71.99 72.03 72.78 73.18 73.22 73.69 73.83 73.97 74.03 74.33 74.68 75.15 75.40 75.45 75.55

IUPAC 66 155 55 80 84 89 56 92 60 90 101 113 150 99 152 83 119 86 112 108 145 125 97 79 116 148 78 87 136 117 115 85 111 154 110 81 120 82 151 135 77 144

Retention Time 75.65 75.83 76.58 77.19 77.48 77.52 77.69 77.78 78.45 78.64 78.78 79.37 79.43 79.47 80.10 80.57 80.64 80.87 80.89 81.13 81.15 81.18 81.48 81.87 82.12 82.63 82.78 82.85 82.94 83.33 83.48 83.54 83.89 83.96 84.44 84.67 84.93 85.61 85.89 86.21 86.47 86.69

IUPAC 147 149 139 143 140 124 107 123 109 134 106 142 188 131 118 133 184 122 114 165 146 161 132 168 153 179 105 141 176 186 137 127 130 163 164 138 160 129 178 158 175 182

Retention Time 87.25 87.53 87.82 87.83 88.20 88.29 88.69 88.81 88.93 89.02 89.04 89.27 89.54 89.62 89.71 90.17 90.67 90.73 91.05 91.17 91.26 91.66 91.86 92.22 92.28 92.93 93.48 93.90 94.08 94.75 95.11 95.16 95.41 95.76 95.77 96.35 96.50 96.58 96.87 96.99 97.74 97.78

Retention IUPAC 187 166 183 126 159 128 185 162 174 181 167 202 177 171 201 204 197 173 156 172 157 192 180 200 193 191 170 198 199 190 196 169 203 208 189 207 195 194 205 206 209

Time 98.18 98.85 99.04 99.87 100.21 100.37 100.70 101.00 101.25 101.94 101.95 102.58 102.70 103.36 103.39 103.47 103.64 105.18 105.30 105.56 105.72 106.05 106.68 106.76 107.01 107.69 110.82 110.90 111.22 111.48 112.14 112.22 112.33 115.39 116.61 116.62 116.65 120.33 121.34 124.75 128.32

GC and GC/MS

www.agilent.com/chem

361

GC and GC/MS Applications


Environmental-Semivolatiles
Congeners in DIN Method PCBs
Column:
P/N: Carrier: Oven: Injector:

DB-XLB
30 m x 0.25 mm id, 0.5 m 1 m x 0.53 mm id Retention Gap 122-1236 Helium at 34.2 cm/sec, measured at 150C 100C for 1 min 100-320C at 5.6/min Hot On-column, 250C, Split Flow 100 mL/min 2 L dilute Aroclor mixture MSD, SIM of 221.9, 255.9, 291.9, 325.8, 359.8, 395.8, 429.7, 463.7 300C transfer line

Extended Temperature Program Resolving Congeners 52 and 138


Column:
P/N: Carrier: Oven: Injector:

DB-XLB
30 m x 0.25 mm id, 0.5 m 1 m x 0.53 mm id Retention Gap j122-1236 Helium at 34.2 cm/sec, measured at 150C 100C for 1 min 100-275C at 1.6/min Hot On-column, 250C, Split Flow 100 mL/min 2 L dilute Aroclor mixture MSD, SIM of 221.9, 255.9, 291.9, 325.8, 359.8, 395.8, 429.7, 463.7 300C transfer line

Detector:

Detector:

GC and GC/MS

C370

C371

362

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GC and GC/MS Applications


Environmental-Semivolatiles
PCBs by EPA Method 8082
Column:
P/N: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. IUPAC 1 Tetrachloro-m-xylene (IS/SS) IUPAC 5 IUPAC 18 IUPAC 31 IUPAC 52 IUPAC 44 IUPAC 66 IUPAC 101 IUPAC 87 IUPAC 110 IUPAC 151 IUPAC 153 IUPAC 141 IUPAC 137 IUPAC 187 IUPAC 183 IUPAC 180 IUPAC 170 IUPAC 206 Decachlorobiphenyl (IS/SS)

DB-35ms
30m x 0.32 mm id, 0.25 m 123-3832

Column:
P/N: Carrier: Oven:

DB-XLB
30 m x 0.32 mm id, 0.50 m 123-1236 Helium at 45 cm/sec (EPC in constant flow mode) 110C for 0.5 min 110-320C at 15C/min 320C for 5 min Splitless, 250C 30 sec purge activation time 50 pg per component ECD, 350C Nitrogen makeup gas (column + makeup flow = 30 mL/min constant flow)

Injector:

Detector:

14 15 18

19

DB-35ms

11 10 12

17 16

20

8 9 5 6 3 4 7

13

22

GC and GC/MS

C790

10 Time (min)

12

14

16

DB-XLB

15 14 17 16 13 9

19 18

10 11 12 8 7 5 6 3 4

20

21

6
C791

10 Time (min)

12

14

16

www.agilent.com/chem

363

GC and GC/MS Applications


Environmental-Semivolatiles
Chlordane
Column:
P/N: Carrier: Oven:

Toxaphene
Column:
P/N: Carrier: Oven:

DB-35ms
30m x 0.32 mm id, 0.25 m film thickness 123-3832 Helium at 45 cm/sec (EPC in constant flow mode) 110C for 0.5 min 110-320C at 15C/min 320C for 5 min Splitless, 250C 30 sec purge activation time 1 g/mL per component in Methanol ECD, 350C Nitrogen makeup gas (column + makeup flow = 30 mL/min constant flow)

DB-35ms
30m x 0.32 mm id, 0.25 m film thickness 123-3832 Helium at 45 cm/sec (EPC in constant flow mode) 110C for 0.5 min 110-320C at 15C/min 320C for 5 min Splitless, 250C 30 sec purge activation time 1 g/mL per component in Methanol ECD, 350C Nitrogen makeup gas (column + makeup flow = 30 mL/min constant flow)

Injector:

Injector:

Detector:

Detector:

DB-35ms

DB-35ms

GC and GC/MS

4
C1083

10 Time (min)

12

14

16

4
C1085

10 12 Time (min)

14

16

18

Pyrethrins
Chromatogram courtesy of Khan Nguyen and Richard Moorman of Sandoz Agro Inc.

Column:
P/N: Carrier: Oven:

DB-1

30 m x 0.32 mm id, 0.25 m 123-1032 Helium at 39 cm/sec, measured at 150C 180C for 11 min 180-200C at 10/min 200C for 8 min 200-210C at 10/min 210C for 18 min 210-245C at 30/min 245C for 4 min Injector: Split 1:20,1 L, 250C Detector: FID, 300C Helium makeup gas at 30 mL/min 1. Heptadecane 2. Octadecane 3. Endo-MGK 264 4. Exo-MGK 264 5. Methoprene 6. Cinerin I 7. Jasmolin I 8. Pyrethrin I 9. BPE (PB) 10. Cinerin II 11. Jasmolin II 12. Pyrethrin II MGK 264 = N-Octylbicycloheptene dicarboximide BPE = Butylcarbityl 6-propylpiperonyl ether

C525

364

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GC and GC/MS Applications


Environmental-Semivolatiles
Organotin Compounds
Column:
P/N: Carrier: Oven: Injection: Detector:

Organomercury Compounds
Column:
1. 2. 3. 4. 5. 6. 7. Dimethylpentyltin Tetrabutyltin Methtyltripentyltin Tributylpentyltin Dibutylpentyltin Butlypentyltin Tetrapentyltin P/N: Carrier: Oven: Injection: Detector:

HP-1
25 m x 0.32 mm x 0.17 m 19091Z-012 Helium, 100 kPa 50C for 1 min 50-260C at 15C/min Splitless, 1 L AED 330C

HP-5
25 m x 0.2 mm id, 0.11 m 19091J-002 Helium, 0.75 mL/min constant flow 80C for 4 min 80-300C at 5C/min Splitless, inlet 300C, 1 L AED 300C, Tin selective at 303.4 mm

Sn (303 nm)
5 2 3 4 6 7

MeHgPh 40 pg

Ph2Hg 38 pg

7
C1031

9 Time (min)

10

11

12

20
C1029

25

30 Time (min)

35

40

45

GC and GC/MS

Organotin Compounds
Column:
P/N: Carrier: Oven: Injection: Detector:

HP-5
25 m x 0.2 mm id, 0.11m 19091J-002 Helium, 0.75 mL/min constant flow 60-360C at 5C/min Splitless, 1 L, inlet 300C AED 300C, Hg selective at 254 mm

Can't find what you're looking for?


Save time and avoid trial and error by accessing pre-defined, high-quality applications.
Oct2SnPh

Consult Agilent's online, searchable Chromatogram Database


Containing hundreds of commonly analyzed compounds, this resource helps chromatographers select the right analytical technique, column and conditions for an application. Search the extensive database for GC, LC and CE applications by compound name or CAS number.

Standard Solution
Bu4Sn Bu2SnPh Bu2SnPh2 BuSnPh2

Solvent Vent

10

20

30 Time (min)

40

50

Harbor Sediment
Me2SnPh 10 pg

BuSnPh2 35 pg Bu4Sn 14 pg Bu SnPh 2 2 17 pg Bu2 SnPh2 78 pg Ph4Sn Oct SnPh 2 35 pg

Solvent Vent 0 C1032 10 20 30 Time (min)

To access the Chromatogram Database go to www.agilent.com/chem and click on "Library."


50

40

www.agilent.com/chem

365

GC and GC/MS Applications


Environmental-Semivolatiles
USEPA Method 8270 Semivolatile Compounds
Column:
P/N: Carrier: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. n-Nitrosodimethylamine Pyridine 2-Fluorophenol Phenol-d5 Phenol Aniline bis(2-Chloroethyl) ether 2-Chlorophenol 1,3-Dichlorobenzene 1,4-Dichlorobenzene-d4 1,4-Dichlorobenzene Benzyl alcohol 1,2-Dichlorobenzene 2-Methylphenol bis(2-Chloroisopropyl) ether 4-Methylphenol n-Nitroso-di-n-propylamine Hexachloroethane Nitrobenzene-d5 Nitrobenzene Isophorone 2-Nitrophenol 2,4-Dimethylphenol Benzoic acid bis(2-Chloroethoxy) methane 2,4-Dichlorophenol 1,2,4-Trichlorobenzene Naphthalene-d8 29. 30. 31. 32. 33. 34. 35. 36. 37. 38. 39. 40. 41. 42. 43. 44. 45. 46. 47. 48. 49. 50. 51. 52. 53. 54. 55. 56. Naphthalene 4-Chloroaniline Hexachlorobutadiene 4-Chloro-3-methylphenol 2-Methylnaphthalene Hexachlorocyclopentadiene 2,4,6-Trichlorophenol 2,4,5-Trichlorophenol 2-Fluorobiphenyl 2-Chloronaphthalene 2-Nitroaniline Dimethylphthalate 2,6-Dinitrotoluene Acenaphthylene 3-Nitroaniline Acenaphthene-d10 Acenaphthene 2,4-Dinitrophenol 4-Nitrophenol Dibenzofuran 2,4-Dinitrotoluene Diethylphthalate 4-Chlorophenyl-phenyl ether Fluorene 4-Nitroaniline 4,6-Dinitro-2-methylphenol n-Nitrosodiphenylamine Azobenzene 57. 58. 59. 60. 61. 62. 63. 64. 65. 66. 67. 68. 69. 70. 71. 72. 73. 74. 75. 76. 77. 78. 79. 80. 81. 82. 83. 2,4,6-Tribromophenol 4-Bromophenyl-phenylether Hexachlorobenzene Pentachlorophenol Phenanthrene-d10 Phenanthrene Anthracene Carbazole Di-n-butylphthalate Fluoranthene Pyrene Terphenyl-d14 Benzidine Butylbenzylphthalate 3,3'-Dichlorobenzidine Benzo[a]anthracene Chrysene-d12 Chrysene bis(2-Ethylhexyl)phthalate Di-n-octylphthalate Benzo[b]fluoranthene Benzo[k]fluoranthene Benzo[a]pyrene Perylene-d12 Indeno[1,2,3-cd]pyrene Dibenz[a,h]anthracene Benzo[g,h,I]perylene

HP-5ms
30 m x 0.25 mm id, 0.5 um 19091S-133 Ramped flow 1.2 mL/min for 0.0 min ramp at 99 mL/min to 2.0 mL/min 2.0 mL/min for 0.35 min ramp at 10 mL/min to 1.2 mL/min 40C for 1.0 min 40-100C at 15C/min 100-240C at 20C/min 240-310C at 10C/min Splitless, 250C 30 mL/min purge flow at 0.35 min 1 uL of 50 ng standard 5973 MSD, 310C transfer line Scan range 35-500 amu, 3.25 scans/sec

Oven:

Inlet: Sample: Detector:

GC and GC/MS

75 45 37 22,23 29 33 38 32 16 4,5 21 28 35 36 42 40 44 41
43

52 48 51 56 55 50

62 63 64

65

66

67 70 68,69 74 76

58 60 72 73

49

53 54 59 60 71 77

78

3 1,2

12 26 30 14 13 25 27 31 11 20 10 19 9 17 8 15 6 18 7

39
47

46

79 57 80 82 81 83

34

24

10

12

14 Time (min)

16

18

20

22

24

C1080

366

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GC and GC/MS Applications


Environmental-Semivolatiles
EPA Method 525.2
Column: DB-5ms
P/N: Carrier: 30 m x 0.25 mm id, 0.25 m 122-5532 Helium, at 32 cm/sec, measured at 45C, constant flow mode 45C for 1 min 45-130C at 30/min 130C for 3 min 130-180C at 12/min 180-240C at 7/min 240-325C at 12/min 325C for 5 min Splitless 1.0 min purge activation time, 300C FocusLiner MSD, 325C transfer line full scan at m/z 45-450 Composite mixture of Accustandard Method 525.2 standards (M-525.2-SV-ASL, M-525.2-FS-ASL, M-525.2-CP-ASL, M-525.2-NP1-ASL, M-525.2-NP2-ASL): target compounds at 2 ng/L, IS/SS at 5 ng/L Peak No./Compound 28. 2,3-Dichlorobiphenyl 29. Hexachlorobenzene 30. Gesatamine (aka Atraton) 31. Prometon 32. Atrazine 33. Simazine 34. -BHC 35. Pentachlorophenol 36. Propazine 37. -BHC 38. Terbufos 39. Pronamide 40. Diazinon 41. Phenanthrene-d10 (IS #2) 42. Chlorothalonil 43. Phenanthrene 44. Terbacil 45. Methyl paraoxon 46. Disulfoton 47. Anthracene 48. -BHC 49. 2,4,5-Trichlorobiphenyl 50. Metribuzin 51. Alachlor 52. Simetryn 53. Ametryn 54. Heptachlor 55. Prometryne 56. Prebane (aka Terbutryne) 57. Bromacil 58. Di-n-butyl phthalate 59 2,2',4,4'-Tetrachlorobiphenyl 60. Metolachlor 61. Dursban (aka Chlorpyrifos) 62. Cyanazine 63. Dacthal (aka DCPA methyl ester) 64. Aldrin 65. Triadimefon 66. Diphenamid 67. MGK-264 (isomer A) 68. MGK-264 (isomer B) 69. Heptachlor epoxide 70. 2,2',3',4,6-Pentachlorobiphenyl 71. Merphos 72. -Chlordane 73. Tetrachlorvinphos (aka Stirifos) RT 13.74 13.77 13.99 14.14 14.26 14.27 14.28 14.35 14.35 14.52 14.62 14.69 14.76 14.85 14.89 14.92 15.02 15.04 15.05 15.06 15.20 15.59 15.95 16.14 16.23 16.33 16.36 16.40 16.72 16.79 16.90 17.02 17.11 17.15 17.23 17.27 17.29 17.43 17.73 17.78 18.11 18.28 18.34 18.36 18.88 18.95 m/z 222/152 284 196/169 225/168 200/215 201/186 181 266 214/172 181 57 173 137/179 188 266 178 161 109 88 178 181 256 198 160 213 227/170 100 241/184 226/185 205 149 292 162 197/97 225/68 301 66 57 72/167 164/66 164 81 326 209/153 373 109 Peak No./Compound 74. Butachlor 75. Pyrene-d10 (SS) 76. Pyrene 77. -Chlordane 78. Endosulfan I 79. trans-Nonachlor 80. Fenamiphos 81. Napropamide 82. Tricyclazole 83. p,p-DDE 84. DEF 85. 2,2',4,4',5,6'-Hexachlorobiphenyl 86. Dieldrin 87. Carboxin 88. Endrin 89. Chlorobenzilate 90. Endosulfan II 91. p,p-DDD 92. Endrin aldehyde 93. Norflurazon 94. Benzyl butyl phthalate 95. Endosulfan sulfate 96. p,p-DDT 97. Hexazinone 98. bis (2-Ethylhexyl) adipate 99. Triphenylphosphate (SS) 100. Endrin ketone (breakdown product) 101. 2,2',3,3',4,4',6-Heptachlorobiphenyl 102. Benz[a]anthracene 101. Chrysene-d12 (IS #3) 104. 2,2',3,3',4,5',6,6'-Octachlorobiphenyl 105. Methoxychlor 106. Chrysene 107. bis(2-Ethylhexyl) phthalate 108. Fenarimol 109. cis-Permethrin 110. trans-Permethrin 111. Benzo[b]fluoranthene 112. Benzo[k]fluoranthene 113. Fluridone 114. Benzo[a]pyrene 115. Perylene-d12 (SS) 116. Indeno[1,2,3-c,d]pyrene 117. Dibenz[a,h]anthracene 118. Benzo[g,h,i]perylene RT 19.03 19.13 19.18 19.21 19.22 19.28 19.33 19.39 19.61 19.76 19.84 19.90 19.92 19.97 20.43 20.56 20.68 20.77 21.01 21.36 21.49 21.53 21.61 21.68 21.87 21.98 22.52 22.59 22.66 22.68 22.70 22.73 22.74 23.10 23.80 24.38 24.50 25.06 25.12 25.66 25.67 25.78 27.63 27.69 28.11 m/z 176/160 212 202 375/373 195 409 303/154 72 189 246 57/169 360 79 143 67/81 139 195 235/165 67 145 149 272 235/165 171 129 326/325 67/317 394/396 228 240 430/428 227 228 149 139 183 183 252 252 328 252 264 276 278 276

Oven:

Injector: Detector: Sample:

Peak No./Compound 1. Isophorone 2. 1,3-Dimethyl-2nitrobenzene (SS) 3. Dichlorvos 4. Hexachlorocyclo-pentadiene 5. EPTC 6. Mevinphos 7. Butylate 8. Vernolate 9. Dimethyl phthalate 10. Terrazole (aka Etridazole) 11. 2,6-Dinitrotoluene 12. Tillam (aka Pebulate) 13. Acenaphthylene 14. Acenaphthene-d10 (IS #1) 15. Chloroneb 16. 2-Chlorobiphenyl 17. Tebuthiuron 18. 2,4-Dinitrotoluene 19. Molinate 20. Diethyl phthalate 21. Fluorene 22. Propachlor 23. Ethoprop 24. Cycloate 25. Chlorpropham 26. Trifluralin 27. -BHC

RT 5.85 6.65 7.41 8.87 9.17 10.09 10.18 10.42 10.45 10.47 10.56 10.61 10.65 11.00 11.17 11.19 11.37 11.51 11.68 12.21 12.35 12.46 12.82 12.86 13.08 13.14 13.69

m/z 82 134 109 237 128 127 57/146 128 163 211/183 165 128 152 164 191 188 156 165 126 149 166 120 158 83/154 127 306 181

GC and GC/MS

C741

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367

GC and GC/MS Applications


Environmental-Semivolatiles
EPA Method 8061 (Phthalate Esters)
Column:
P/N: Carrier: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. Dimethyl phthalate Diethyl phthalate Benzyl benzoate (IS) Diisobutyl phthalate Di-n-butyl phthalate bis(4-Methoxyethyl) phthalate bis(4-Methyl-2-pentyl) phthalate *Two isomers bis(2-Ethoxyethyl) phthalate Diamyl phthalate Dihexyl phthalate Butyl benzyl phthalate 12. 13. 14. 15. 16. 17. 18. 19. 20. Hexyl 2-ethylhexyl phthalate bis(2-n-Butoxyethyl) phthalate Dicyclohexyl phthalate bis(2-Ethylhexyl) phthalate Diphenyl phthalate (SS Diphenyl isophthalate (SS) Di-n-octyl phthalate Dibenzyl phthalate (SS) Dinonyl phthalate

DB-5ms
20 m x 0.18 mm id, 0.18 m 121-5522 Helium at 49 cm/sec, measured at 80C constant flow program 80C for 0.5 min 80-160C at 30/min 160-320C at 15/min Splitless, 300C 30 sec purge activation time 1 L of 20 ng/L Method 8061 mixture (Accustandard) in hexane MSD, m/z 50-400 full scan 325C transfer line

Oven:

Injector:

* Two isomers SS - Surrogate Standard

Detector:

GC and GC/MS

C730

PAHs
Column:
P/N: Guard Column: P/N: Carrier: Oven:

DB-17ms
30 m x 0.25 mm id, 0.25 m 122-4732 1 m x 0.53 mm id 160-2535 Helium at: 34.1 cm/sec, measured at 150C 95C for 0.5 min 95-340C at 5/min 340C for 5 min Split 1:40, 300C 2 L, PAH standard MSD, 340C transfer line Scan 80-330 amu

Injector: Detector:

8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33.

Name 1. Naphthalene 2. 2-Methylnaphthalene 3. 1-Methylnaphthalene 4. Azulene 5. Acenaphthene 6. Biphenyl 7. 2,6-Dimethylnaphthalene

Ions 128 142,141 142,141 128 154 154 156,155

Acenaphthalene Dibenzofuran Dibenzo-p-dioxin Fluorene 1-Nitronaphthalene 9,10-Dihydroanthracene 2-Nitronaphthalene 2-Nitrobiphenyl Dibenzothiophene Phenanthrene Anthracene 3-Nitrobiphenyl 4-Nitrobiphenyl 5,6-Benzoquinoline Carbazole 2-Methylanthracene 1,2,3,4-Tetrahydrofluoranthene 2-Phenylnaphthalene 9-Methylanthracene 3,6-Dimethylphenanthrene 1,3-Dinitronaphthalene 1,5-Dinitronaphthalene Fluoranthene 2,2'-Dinitrobiphenyl Pyrene 2-Methylfluoranthene

152 168,139 184 166,165 127,173 179,180 127,173 152,115 184 178 178 199,152 199,152 179 167 192,191 178,206 204 192,191 206,191 126,218 218,114 202 198,139 202 216,215

34. 2,3-Benzofluorene 35. Dodecahydrotriphenylene 36. 1-Amino-4nitronaphthalene 37. 9-Phenylanthracene 38. 1,2-Benzanthracene 39. Chrysene 40. Benz[a]anthracene7,12-dione 41. 2,7-Dinitrofluorene 42. Benzo[b]fluoroanthene 43. Benzo[k]fluoranthene 44. 7,12-Dimethylbenz [a]anthracene 45. Benzo[e]pyrene 46. Benzo[a]pyrene 47. Perylene 48. 3-Methylcholanthrene 49. 9,10-Diphenylanthracene 50. 1,2,3,4-Dibenzanthracene 51. 1,2,5,6-Dibenzanthracene 52. Benzo[g,h,i]perylene

216,215 240,198 188,115 254,253 228 240 258,202 256,163 252 252 256,241 252 252 252 268 330 278 278 276

C467A

368

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GC and GC/MS Applications


Environmental-Semivolatiles
Phenols
Column:
P/N: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. Phenol 2-Chlorophenol 2-Methylphenol 4-Methylphenol 3-Methylphenol 2-Chloro-5-methylphenol 2,6-Dimethylphenol 2-Nitrophenol 2,4-Dimethylphenol 2,5-Dimethylphenol 2,4-Dichlorophenol 2,3-Dimethylphenol 2,5-Dichlorophenol 2,3-Dichlorophenol 2-Chlorophenol 4-Chlorophenol 3,4-Dimethylphenol 2,6-Dichlorophenol 4-Chloro-2-methylphenol 4-Chloro-3-methylphenol 2,3,5-Trichlorophenol 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38. 39. 40. 2,4-Dibromophenol 2,4,6-Trichlorophenol 2,4,5-Trichlorophenol 2,3,4-Trichlorophenol 3,5-Dichlorophenol 2,3,6-Trichlorophenol 3,4,-Dichlorophenol 3-Nitrophenol 2,5-Dinitrophenol 2,4-Dinitrophenol 4-Nitrophenol 2,3,5,6-Tetrachlorophenol 2,3,4,5-Tetrachlorophenol 2,3,4,6-Tetrachlorophenol 3,4,5-Trichlorophenol 2-Methyl-4,6-dinitrophenol Pentachlorophenol Dinoseb 2-Cyclohexyl-4,6-dinitrophenol

DB-5ms
30 m x 0.25 mm id, 0.25 m 122-5532

Column:
P/N: Carrier: Oven:

DB-XLB
30 m x 0.25 mm id, 0.25 m 122-1232 He at 1.2 mL/min Constant Flow 40C for 2.00 min 40-100C at 40C/ min 100C for 0.50 min 100-140C at 2C/min 140-340C at 30C/min Pulsed Splitless, 200C Pulse Pressure & Time: 25.0 psi for 1.00 min Purge Flow & Time: 50.0 mL/min for 0.25 min Gas Saver Flow & Time: 20.0 mL/min for 3.00 min MSD Transfer Line Temp at 320C Quadrapole at 150C 12, 13 Source at 230C

Injector:

Detector:

DB-5ms
15, 17 22, 23

36, 37

24 26 2728 4, 5 6 1 2 3 7 9 10 14 18 11 19 20 21 25 32 29, 30

34 35 33 38 39 40

GC and GC/MS

31

5 C1081

9 Time (min)

10

11

12

36

DB-XLB
38 39 12 7 6

8,10

11,13 17 30,32 14 22 19 16 18 20 29 21 23,24 25 26 27 28

34

35 33 37

40

2 1

3 5 4 9

15

31

6 C1082

10

12

14

16

18 Time (min)

20

22

24

26

28

30

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369

GC and GC/MS Applications


Environmental-Semivolatiles
EPA 552.2
Column:
P/N:

DB-35ms
30m x 0.32 mm id, 0.25 m film thickness 123-3832 30 m x 0.32 mm id, 0.50 m film thickness 123-1236 Helium at 45 cm/sec (EPC in constant flow mode) 40C for 0.5 min 40-200C at 15C/min 200C for 2 min Splitless, 250C 30 sec purge activation time 50 pg per component ECD, 350C Nitrogen makeup gas (column + makeup flow = 30 mL/min constant flow)
11

DB-XLB
P/N: Carrier: Oven:

Injector:

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12.

Chloroacetic acid Bromoacetic acid Dichloroacetic acid Dalapon Trichloroacetic acid 1,2,3-Trichloropropane (IS) Bromochloroacetic acid Bromodichloroacetic acid Dibromoacetic acid 2,3-Dibromopropionic acid (SS) Chlorodibromoacetic Tribromoacetic acid
12

Detector:

DB-35ms
9 7

2, 3

4 6

min

C788

GC and GC/MS

DB-XLB
12 11

8, 9 7

3 5 2 4 1 6 10

min

C788

370

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GC and GC/MS Applications


Environmental-Volatiles
EPA Volatiles by GC/MS (Split Injector)
Column:
P/N: Carrier: Oven: 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. Acetone Iodomethane Carbon disulfide Allyl chloride Methylene chloride Acrylonitrile Methyl-tert-butyl ether trans-1,2-Dichloroethene Hexane 1,1-Dichloroethane 2-Butanone cis-1,2-Dichloroethene 2,2-Dichloropropane Propionitrile Methyl acrylate Methacrylonitrile 25. 26. 27. 28. 29. Bromochloromethane Tetrahydrofuran Chloroform Pentafluorobenzene (IS) 1,1,1-Trichloroethane 30. 31. 32. 33. 34. 35. 36. 37. 38. 39. 40. 1-Chlorobutane 1,1-Dichloropropene Carbon tetrachloride Benzene 1,2-Dichloroethane 2,2-Dimethylhexane Fluorobenzene (IS) 1,4-Difluorobenzene (IS) Trichloroethene 1,2-Dichloropropane Methyl methacrylate

DB-VRX
60 m x 0.25 mm id, 1.4 m 122-1564 Helium at 30 cm/sec, measured at 45C 45C for 10 min 45-190C at 12/min 190C for 2 min 190-225C at 6/min 225C for 1 min Purge and trap (O.I.A. 4560) 40 ppb per each component in 5 mL water Helium for 11 min at 40 mL/min Tenax/Silica Gel/Carbosieve 0.6 min at 220C Split injector at 110C with split flow set at 30 mL/min MSD, 235C transfer line full scan 35-260 amu (m/z 44 subtracted)

61,62

68,63

Injector: Sample: Purge: Trap: Desorb: Interface: Detector:

60 64 48 57 58

70 71 66 72 73 67 75

Compound list for both chromatograms (NOTE: Some compounds not present in both chromatograms) 1. Dichlorodifluoromethane 2. Chloromethane 3. Vinyl chloride 4. Bromomethane 5. Chloroethane 6. Trichlorofluoromethane 7. Diethyl ether 8. 1,1-Dichloroethene
10,14 13 12 2 3 4 1 7 5 6 9 16 15 11 18,22 19 20 27 24 25 21

74 79 78

53 33 29,30 44 34 28 23 26 31 32 41 36 37 38 42,43 47,45 49 39 40 46

50 69

76

80 83 81 82 84

51 52 54 55 56

59 77

89 85 88 90 91 86

65

GC and GC/MS

87

10

15 Time (min)

20

25

30

C378B

Column:
P/N: Carrier: Oven:

DB-624
60 m x 0.25 mm id, 1.4 m 122-1364 Helium at 31 cm/sec, measured at 40C 40C for 3 min 40-90C at 8/min 90C for 4 min 90-200C at 6/min 200C for 5 min Purge and trap (O.I.A. 4560) 40 ppb per each component in 5 mL water Helium for 11 min at 40 mL/min Tenax/Silica Gel/Carbosieve 0.6 min at 220C Split injector at 110C with split flow set at 30 mL/min MSD, 235C transfer line, full scan 35-260 amu (m/z 44 subtracted)
33,34

Injector: Sample: Purge: Trap: Desorb: Interface: Detector:

53. 54. 55. 56. 57. 58. 59. 60. 61. 62. 63. 64. 65. 66. 67. 68. 69.

1,3-Dichloropropane 2-Hexanone Dibromochloromethane 1,2-Dibromoethane 1-Chloro-3-fluorobenzene (IS) Chlorobenzene 1,1,1,2-Tetrachloroethane Ethylbenzene m-Xylene p-Xylene o-Xylene Styrene Bromoform Isopropylbenzene 4-Bromofluorobenzene (SS) 1,1,2,2-Tetrachloroethane Bromobenzene
48

70. 71. 72. 73. 74.


61,62

1,2,3-Trichloropropane trans-1,4-Dichloro-2-butene n-Propylbenzene 2-Chlorotoluene 1,3,5-Trimethylbenzene

63,64

75. 76. 77. 78. 79. 80. 81. 82. 83. 84. 85. 86. 87. 88. 89. 90. 91.

4-Chlorotoluene tert-Butylbenzene Pentachloroethane 1,2,4-Trimethylbenzene sec-Butylbenzene 1,3-Dichlorobenzene p-Isopropyltoluene 1,4-Dichlorobenzene n-Butylbenzene 1,2-Dichlorobenzene Hexachloroethane 1,2-Dibromo-3-chloropropane Nitrobenzene 1,2,4-Trichlorobenzene Hexachlorobutadiene Naphthalene 1,2,3-Trichlorobenzene

72 52,53 60 66 73 74 70,71 67 75

79 80 78 76 81 82 84 90 85 88 91 83

Compound list continued 41. Dibromomethane 42. Bromodichloromethane 43. 2-Nitropropane 44. Chloroacetonitrile 45. cis-1,3-Dichloropropene 46. 4-Methyl-2-pentanone 47. 1,1-Dichloro-2-propanone 48. Toluene 49. trans-1,3-Dichloropropene 50. Ethyl methacrylate 51. 1,1,2-Trichloroethane 52. Tetrachloroethene
4 1 23 5 6

15,16 14 11 10 18 8,9 12 7 17 35 43,44 47 13 24 23 19,20,21,22 31,32 26,27 38 25 29 36 39 41 42 46 54 30 40 45 50 49 51 55 56 59

58 69 68

65

89

86

87

10

15 Time (min)

20

25

30

35

C378A

www.agilent.com/chem

371

GC and GC/MS Applications


Environmental-Volatiles
High Speed VOC EPA 8260
Column:
P/N: Carrier: Oven: 32. 33. 34. 35. 36. 37. 38. 39. 40. 41. 42. 43. 44. 45. 46. 47. 48. 49. 50. 51. 52. 53. 54. 55. 56. 57. 58. 59. 60. 61. 62. 63. 64. 65. 66. 67. 68. 69. 70. 71. 72. 73. 74. 2,2-Dichloropropane Ethyl acetate Ethyl-tert-butyl ether Methyl acrylate Dibromofluoromethane IS Isobutanol Dichloroethane-d4 IS Pentafluorobenzene 1,2-Dichloroethane 1,1,1-Trichloroethane 1-Chlorobutane Crotonaldehyde 2-Chloroethanol 1,1-Dichloropropene 1-Butanol Carbon tetrachloride Chloroacetonitrile Benzene tert-Amylmethyl ether Fluorobenzene IS 2-Pentanone Dibromomethane 1,2-Dichloropropane Trichloroethene Bromodichloromethane 2-Nitropropane 1,4-Dioxane Epichlorohydrin Methyl methacrylate cis-1,3-Dichloropropene Propiolactone Bromoacetone Pyridine trans-1,3-Dichloropropene 1,1,2-Trichloroethane Toluene-d8 IS Toluene 1,3-Dichloropropane Paraldehyde Ethyl methacrylate Dibromochloromethane 3-Chloropropionitrile 1,2-Dibromoethane 41,42 39,40 46 27 26 2.0 3.0 37 38 43,44 50 52 58 4.0 62 45 47 48 51 53 75. Tetrachloroethene 76. 1,1,1,2-Tetrachloroethane 77. 1-Chlorohexane 78. Chlorobenzene 79. Ethylbenzene 80. Bromoform 81. m-Xylene 82. p-Xylene 83. trans-Dichlorobutene 84. 1,3-Dichloro-2-propanol 85. Styrene 86. 1,1,2,2-Tetrachloroethane 87. o-Xylene 88. 1,2,3-Trichlropropane 89. cis-Dichlorobutene 90. 4-Bromofluorobenzene IS 91. Isopropylbenzene 92. Bromobenzene 93. Propylbenzene 94. 2-Chlorotoluene 95. 4-Chlorotoluene 96. 1,3,5-Trimethylbenzene 97. Pentachloroethane 98. tert-Butylbenzene 99. 1,2,4-Trimethylbenzene 100. sec-Butylbenzene 101. 1,3-Dichlorobenzene 102. Benzylchloride 103. 1,4-Dichlorobenzene-d4 IS 104. 1,4-Dichlorobenzene 105. Isopropyltoluene 106. 1,2-Dichlorobenzene 107. Butylbenzene 108. 1,2-Dibromo-3-chloropropane 109. Hexachloroethane 110. Nitrobenzene 111. 1,2,4-Trichlorobenzene 112. Naphthalene 113. Hexachlorobutadiene 114. 1,2,3-Trichlorobenzene 55 54 56,57 59,60 61

DB-VRX
20 m x 0.18 mm id, 1.0 m film 121-1524 Helium at 55 cm/sec (1.5 mL/min) 45C for 3.0 minutes 45-190C at 36C/min 190-225C at 20C/min 225C for 0.5 min Tekmar 3100 Purge and Trap Vocarb 3000 5 mL 11 minutes 245C 1 minute at 250C 10 minutes at 260C 100C

Interface: Agilent 5973 MSD Scan range: Scan rate: Quad temperature: Source temperature: Transfer line temp:

Split injector at 150C, 60:1 split ratio 35-260 amu 3.25 scans/sec 150C 200C 200C

Injector: Trap: Sample volume: Purge: Desorb preheat: Desorb: Bake: Line and valve temp: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31.

Sample Concentration Halogentated and aromatic analytes at 40 ppb Internal standards at 20 ppb Polar analytes (i.e., ethers, alcohols and ketones at 100-800 ppb) 28,29

GC and GC/MS

Dichlorodifluoromethane Chloromethane Hydroxypropionitrile Vinyl chloride Bromomethane Chloroethane Ethanol Acetonitrile Acrolein Trichlorofluoromethane Isopropyl alcohol Acetone Ethyl ether 1,1-Dichloroethene tert-Butyl alcohol Acrylonitrile Methylene chloride Allyl chloride Allyl alcohol 1-Propanol Propargyl alcohol trans-1,2-Dichloroethene MTBE 1,1-Dichloroethane Propionitrile 2-Butanone Diisopropyl ether cis-1,2-Dichloroethene Methacrylonitrile Bromochloromethane Chloroform

Ideal for 5973 MSD

8-10

16

14,15 17 18

22

49

23

2 1 3,4

5 13 6 7 11 12 19 1.0 21 20 25 24 32-34 30 31

35,36

C797a

Time (min)
98 80-82 96,97 77,78 99

101,102 105 100


103,104

86,87

90,91 93 107 98 95 94 92 109 106

79 68

84,85

6.4

6.5

6.6
111

114

113 112

75 76 65 63, 64 66 67 69,70 71 88 72 74 73 83 89 108 110

C797b

5.0

6.0

7.0

8.0

Time (min)

372

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GC and GC/MS Applications


Environmental-Volatiles
Extended Analyte List for EPA Method 8021 DB-624/VRX Dual Column
Columns: DB-624
P/N: 75 m x 0.45 mm id, 2.55 m 124-1374 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38. 39. 40. Allyl chloride Methylene chloride trans-1,2-Dichloroethene 1,1-Dichloroethane Chloroprene cis-1,2-Dichloroethene 2,2-Dichloropropane Bromochloromethane Chloroform 1,1,1-Trichloroethane Carbon tetrachloride 1,1-Dichloropropene Benzene 1,2-Dichloroethane Fluorobenzene (IS) Trichloroethene 1,2-Dichloropropane Dibromomethane Trifluorotoluene (IS) Bromodichloromethane 2-Chloroethyl vinyl ether cis-1,3-Dichloropropene Toluene trans-1,3-Dichloropropene 1,1,2-Trichloroethane Tetrachloroethene 1,3-Dichloropropane Dibromochloromethane 1,2-Dibromoethane 1-Chloro-3-fluorobenzene (IS) Chlorobenzene 41. 42. 43. 44. 45. 46. 47. 48. 49. 50. 51. 52. 53. 54. 55. 56. 57. 58. 59. 60. 61. 62. 63. 64. 65. 66. 67. 68. 69. 70. 71. 1,1,1,2-Tetrachloroethane Ethylbenzene m-xlyene p-Xylene Styrene o-Xylene Bromoform Isopropylbenzene cis-1,4-Dichlorobutene 1,1,2,2-Tetrachloroethane Bromobenzene 1,2,3-Trichloropropane n-Propylbenzene 2-Chlorotoluene 1,3,5-Trimethylbenzene 4-Chlorotoluene tert-Butylbenzene 1,2,4-Trimethylbenzene sec-Butylbenzene 1,3-Dichlorobenzene p-Isopropyltoluene 1,4-Dichlorobenzene Benzyl chloride n-Butylbenzene 1,2-Dichlorobenzene bis (2-Chloroisopropyl) ether 1,2-Dibromo-3-chloropropane 1,2,4-Trichlorobenzene Hexachlorobutadiene Naphthalene 1,2,3-Trichlorobenzene

DB-VRX
75 m x 0.45 mm id, 2.55 m Guard column: 5 m x 0.53 mm id deactivated fused silica 3-way universal glass union P/N: 124-1574 Carrier: Helium at 9 mL/min, measured at 35C Oven: 35C for 12 min 35-60C at 5/min 60C for 1 min 60-200C at 17/min 200C for 5 min Injector: Purge and trap (O.I.A. 4560) J&W LVI (Low Volume Injector), 150C 20 ppb per component in 5 mL water Trap: VOCARB 3000 Preheat: 175C Desorb: 260C for 1 min Detector A: PID (O.I.A. 4430), 200C Helium makeup gas at 20 mL/min Detector B: ELCD (O.I.A. 4420), with NiCat reaction tube in the halogen mode, 950C reactor temperature

Compound list for all chromatograms 1. Dichlorodifluoromethane 2. Chloromethane 3. Vinyl chloride 4. Bromomethane 5. Chloroethane 6. Trichlorofluoromethane 7. 2-Chloropropane (IS) 8. 1,1-Dichloroethene 9. Iodomethane

GC and GC/MS

Detector B: ELCD
DB-624

DB-VRX

C432

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373

GC and GC/MS Applications


Environmental-Volatiles

Detector A: PID
DB-624

GC and GC/MS
DB-VRX

374

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GC and GC/MS Applications


Environmental-Volatiles
Fast VOA Analysis
Columns: DB-624
P/N: Carrier: Oven: 20 m x 0.18 mm id, 1.0 m 121-1324 Helium at 37 cm/sec, (constant flow mode) 35C for 4 min 35-200C at 15/min 200C for 0.1 min 60-200C at 17/min Purge and trap (Tekmar LSC 3000) 10 ppb per component in 25 mL water Helium for 11 min at 50 mL/min 250C 260C for 2 min 100C 5973 MSD, 250C transfer line Full scan 35 -260 amu 3.25 scans per second 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38. 39. 40. 41. 42. 43. 44. 45.
15 16

Injector: Sample: Purge: Preheat: Desorb: Line temp: Detector:

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14.

Dichlorofluoromethane Chloromethane Vinyl chloride Bromomethane Chloroethane Trichlorofluoromethane 1,1-Dichloroethene Methylene Chloride trans-1,2-Dichloroethene 1,1-Dichloroethane 2,2-Dichloropropane Bromochloromethane Chloroform 1,1,1-Trichloroethane 7
9 8

Ideal for 5973 MSD

Carbon tetrachloride Benzene Fluorobenzene Trichloroethene 1,2-Dichloropropane Dibromomethane Bromodichloromethane cis-1,3-Dichloropropene Toluene trans-1,3-Dichloropropene 1,1,2-Trichloroethane Tetrachloroethene Dibromochloromethane 1,2-Dibromomethane Chlorobenzene 1,1,1,2-Tetrachloroethane Ethylbenzene m-Xylene p-Xylene o-Xylene Bromoform Isopropylbenzene Bromofluorobenzene Bromobenzene n-Propylbenzene 2-Chlorotoluene 1,3,5-Trimethylbenzene tert-Butylbenzene 1,2,4-Trimethylbenzene sec-Butylbenzene 1,3-Dichlorobenzene

46. 47. 48. 49. 50. 51. 52. 53.

48 32,33 41

4-Isopropyltoluene 1,4-Dichlorobenzene 1,2-Dichlorobenzene 1,2-Dibromo-3Chloropropane 1,2,4-Trichlorobenzene Hexachlorobutadiene Napthalene 1,2,3-Trichlorobenzene

29 34 46

39 31 36 40 23

44 42 43

45 51

26 30 38 37

47

50 53

11

18

6 23 4 1 5

13 10 12

14

19 21 17 20

22 24 25 52 27 28 35 49

GC and GC/MS

8 Time (min)

10

11

12

13

14

15

C804

www.agilent.com/chem

375

GC and GC/MS Applications


Environmental-Volatiles
EPA Method 504 by GC/MS
Column:
P/N: Oven: Injector:

EPA Method 504 by GC/MS


Column:
P/N: Oven: Injector:

DB-VRX
30 m x 0.25 mm id, 1.4 m 122-1534 40C for 0.5 min 40-200C at 10/min Splitless, 200C 12 sec purge activation time 25 pg on-column of each component MSD, 220C transfer line, SIM

DB-624
30 m x 0.25 mm id, 1.4 m 122-1334 40C for 0.5 min 40-200C at 10/min Splitless, 200C 12 sec purge activation time 25 pg on-column of each component MSD, 220C transfer line, SIM 6. 7. 8. 9. 10. 11. Bromodichloromethane 1,2-Dibromopropane 1,2-Dibromoethane (EDB) Dibromochloromethane Bromoform 1,2-Dibromo-3-chloropropane (DBCP)

Detector: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11.

Detector: 1. 2. 3. 4. 5.

1,1,1-Trichloroethane Carbon tetrachloride Trichloroethene Chloroform Tetrachloroethene Bromodichloromethane 1,2-Dibromopropane 1,2-Dibromoethane (EDB) Dibromochloromethane Bromoform 1,2-Dibromo-3-chloropropane (DBCP)

1,1,1-Trichloroethane Carbon tetrachloride Trichloroethene Chloroform Tetrachloroethene

GC and GC/MS

C373

C196

EPA Method 551


Column:
P/N: Carrier: Oven:

DB-1
30 m x 0.25 mm id, 1.0 m 122-1033 Helium at 24.8 cm/sec, measured at 150C 35C for 9 min 35-40C at 10/min 40C for 3 min 40-150C at 6/min 150C for 1 min Splitless, 200C 15 sec purge activation time 1 L of 50 pg/L, AccuStandard ECD, 300C

Injector:

Detector:

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19.

Chloroform 1,1,1-Trichloroethane Carbon tetrachloride Trichloroacetonitrile Dichloroacetonitrile Bromodichloromethane Trichloroethene Chloral hydrate 1,1-Dichloro-2-propanone Chloropicrin Dibromochloromethane Bromochloroacetonitrile 1,2-Dibromoethane Tetrachloroethene 1,1,1-Trichloropropanone 1,2-Dibromopropane (IS) Bromoform Dibromoacetonitrile 1,2-Dibromo-3-chloropropane

C201

376

www.agilent.com/chem

GC and GC/MS Applications


Environmental-Volatiles
EPA Method 551.1
Column:
P/N: Carrier: Oven:

DB-5ms
30 m x 0.25 mm id, 1.0 mm 122-5533 Helium at 35 cm/sec, measured at 150C 35C for 9 min 35-40C at 10/min 40C for 3 min 40-150C at 15/min 150C for 5 min Splitless, 200C 0.1 min purge activation time 1 mL of 0.5-10 ng/mL ECD, 325C

Injector:

Detector: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21.

Chloroform 1,1,1-Trichloroethane Carbon tetrachloride Trichloroacetonitrile Trichloroethene Bromodichloromethane Chloral hydrate Dichloroacetonitrile 1,1-Dichloro-2-propanone 1,1,2-Trichloroethane Chloropicrin Dibromochloromethane Tetrachloroethene 1,2-Dibromoethane Bromochloroacetonitrile 1,1,1-Trichloro-2-propanone Bromoform Dibromoacetonitrile 1,2,3-Trichloropropane p-Bromofluorobenzene (IS) 1,2-Dibromo-3-chloropropane

GC and GC/MS

C587

European Red List Volatiles


Column:
P/N: Carrier: Oven: Injector: Detector:

DB-5.625
30 m x 0.25 mm id, 0.5 m 122-5632 Helium at 35 cm/sec, measured at 40C 40C for 2 min 40-140C at 12/min Split 1:50, 250C 1 L of headspace of neat mixture FID, 300C Nitrogen makeup gas at 30 mL/min

Column:
P/N: Carrier: Oven: Injector: Detector:

DB-624
30 m x 0.25 mm id, 1.4 m 122-1334 Helium at 35 cm/sec, measured at 40C 40C for 2 min 40-140C at 12/min Split 1:50, 250C 1 L of headspace of neat mixture FID, 300C Nitrogen makeup gas at 30 mL/min

1. 2. 3. 4. 5. 6. 7. 8.

1,1-Dichloroethylene 1,1-Dichloroethane Chloroform 1,1,1-Trichloroethane 1,2-Dichloroethane Carbon tetrachloride Trichloroethylene Tetrachloroethylene

1. 2. 3. 4. 5. 6. 7. 8.

1,1-Dichloroethylene 1,1-Dichloroethane Chloroform 1,1,1-Trichloroethane 1,2-Dichloroethane Carbon tetrachloride Trichloroethylene Tetrachloroethylene

5 2 4 3 7 8

4 Time (min)

10

C307

C308

www.agilent.com/chem

377

GC and GC/MS Applications


Environmental-Air Analysis
EPA Air Analysis Compendium Method TO-14 Standard
Column:
P/N: Carrier: Oven:

DB-1
60 m x 0.32 mm id, 1.0 m 123-1063 Helium at 25 cm/sec measured off of CO2 at 35C constant flow mode 35C for 5 min 35-120C at 5/min 120-220C at 30/min 220C for 5 min Entech 7100 cryogenic sample preconcentrator 400 mL of a 10 ppbV TO-14 standard and 100 mL of a 20 ppbV IS/SS standard MSD, full scan of m/z 40-250

Injector:

Detector:

Agilent wishes to thank Entech Instruments for providing this chromatogram.

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23.

CO2 Freon 12 (Dichlorodifluoromethane) Chloromethane Freon 114 (1,2-Dichloro-1,1,2,2-tetrafluoroethane) Vinyl chloride Bromomethane Chloroethane Freon 11 (Trichlorofluoromethane) 1,1-Dichloroethene Methylene chloride Freon 113 (1,1,2-Trichloro-1,2,2-trifluoroethane) 1,1-Dichloroethane cis-1,2-Dichloroethene Bromochloromethane (IS) Chloroform 1,2-Dichloroethane 1,1,1-Trichloroethane Benzene Carbon tetrachloride 1,4-Difluorobenzene (IS) 1,2-Dichloropropane Trichloroethene cis-1,3-Dichloropropene

24. 25. 26. 27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38. 39. 40. 41. 42. 43. 44. 45. 46.

trans-1,3-Dichloropropene 1,1,2-Trichloroethane Toluene-d8 (SS) Toluene 1,2-Dibromoethane Tetrachloroethene Chlorobenzene-d5 (SS) Chlorobenzene Ethylbenzene m-Xylene p-Xylene Styrene 1,1,2,2-Tetrachloroethane o-Xylene 4-Bromofluorobenzene (SS) 1,3,5-Trimethylbenzene 1,2,4-Trimethylbenzene 1,3-Dichlorobenzene 1,2-Dichlorobenzene 1,4-Dichlorobenzene 1,2,4-Trichlorobenzene 1,2-Dibromobenzene (IS) Hexachloro-1,3-butadiene

11 22 17 15 8 9 10 14 3 5 6 7 13 12 16 20 23 21 18 19

GC and GC/MS

8 Time (min)

10

11

12

13

14

Ion 91.00 (90.70 to 91.70): 98100107.D

Peak 37 m/z 91 46

Peak 36 m/z 83

39

40 43 41

21.45 21.50 21.55 21.60 21.65 29 32 27 25 31 35 38 30 26 28 24 33

42

34

36 37 44 45

16

17

18

19

20

C731

21 22 Time (min)

23

24

25

26

27

378

www.agilent.com/chem

GC and GC/MS Applications


Environmental-Air Analysis
EPA Air Analysis Method TO-15 (1 ppbV Standard)
Column:
P/N: Carrier: Oven: Peak # 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38. 39. 40. 41. 42. 43. 44. 45. 46. 47. 48. 49. 50. 51. 52. 53. 54. 55. 56. 57. 58. 59. 60. 61. 62. 63. 64. 65. 66. 67. 68. 69. 70. 71. Compound Name Formaldehyde Propene Dichlorodifluoromethane Chloromethane Dichlorotetrafluoroethane Acetaldehyde Vinyl chloride 1,3-Butadiene Bromomethane Chloroethane Bromoethene Trichlorofluoromethane Acetone Propanal Isopropyl alcohol 1,1-Dichloroethene 1,1,2-Trichloro-1,2-2-trifluoroethane Methylene chloride 3-Chloro-1-propene (Allyl chloride) Carbon disulfide trans-1,2-Dichloroethene tert-Butyl methyl ether (MTBE) 1,1-Dichloroethane Vinyl acetate 2-Butanone (MEK) n-Hexane cis-1,2-Dichloroethene Ethyl acetate Bromochloromethane <ISTD> Chloroform Tetrahydrofuran 1,1,1-Trichloroethane 1,2-Dichloroethane Benzene Carbon tetrachloride Cyclohexane 1,4-Difluorobenzene <ISTD> 2,2,4-Trimethylpentane (Isooctane) n-Heptane Trichloroethene 1,2-Dichloropropane 1,4-Dioxane Bromodichloromethane 4-Methyl-2-pentanone (MIBK) cis-1,3-Dichloropropene trans-1,3-Dichloropropene Toluene 1,1,2-Trichloroethane 2-Hexanone Dibromochloromethane Tetrachloroethene 1,2-Dibromoethane Chlorobenzene-d5 <ISTD> Chlorobenzene Ethylbenzene m-Xylene p-Xylene Styrene o-Xylene Bromoform 1,1,2,2-Tetrachloroethane 4-Bromofluorobenzene <SMC> 4-Ethyltoluene 1,3,5-Trimethylbenzene 1,2,4-Trimethylbenzene 1,3-Dichlorobenzene Benzyl chloride 1,4-Dichlorobenzene 1,2-Dichlorobenzene 1,2,4-Trichlorobenzene Hexachlorobutadiene Quantitation Ion 30 41 85 50 85 29 62 39 94 64 106 101 58 29 45 61 101 49 76 76 96 73 63 43 72 57 96 43 128 83 42 97 62 78 117 56 114 57 41 130 63 88 83 43 75 75 91 97 43 129 166 107 117 112 91 91 91 104 91 173 83 95 105 105 105 146 91 146 146 180 225

DB-5ms
60 m x 0.32 mm id, 1.0 m 123-5563 Helium, 1.5 mL/min 35C for 5 min 35-140C at 6C/min 140-220C at 15C/min 220C for 3 min Entech 7100 cryogenic sample preconcentrator 40 cc 10 ppb GC/MS 6890/5973 N Scan 29-180 amu 0-6 min 33-280 amu 6-30 min Electron Impact 70 eV Sample: 400 mL sample load, All compounds at 10 ppbV except Formaldehyde (50 ppbV), Acetaldehyde (20 ppbV), Propanol (20 ppbV), Acetone (30 ppbV), 2-Butanone (30 ppbV)

Injector: Sample: Detector:

37 29

4,5

17 40

34,35 3 12 18,19 13 6,7 8 1,2 10 9 11 14 15 28 31 16 20 21 23,24 22 33 26 27 25 30 32 36

38 39 43 44,45

41

GC and GC/MS

42

4 C1089-a

9 Time (min)

10

11

12

13

14

53

62

71

63 64 55 56 57 51 47 54 58 61

65 66

68 69 70

59

48 50 52 60

67

Agilent wishes to thank Entech Instruments for providing this chromatogram.

46 49

15 C1089-b

16

17

18

19

20

21 22 Time (min)

23

24

25

26

27

28

www.agilent.com/chem

379

GC and GC/MS Applications


Environmental-Air Analysis
50ppb Formaldehyde
Column:
P/N: Carrier: Oven: Injector: Sample: Detector:

Sulfur in Air
Column:
P/N: Carrier: Oven: 1. 2. 3. 4. 5. 6. Formaldehyde Acetaldehyde Acetone-d6 Acetone Propanol 2-Butanone

Injector: Sample: Detector:

DB-5ms
60 m x 0.32 mm id, 1.0 m 123-5563 Helium, 1.5 mL/min 35C for 5 min 35-85C at 10C/min Entech 7100 cryogenic sample preconcentrator 100 cc 50 ppb Formaldehyde/20 ppb others GC/MS 6890/5973 N Scan 29-180 amu 0-6 min 33-280 amu 6-10 min Electron Impact 70 eV

DB-5ms
60 m x 0.32 mm id, 1.0 m 123-5563 Helium, 1.5 mL/min 35C for 5 min 35-140C at 6C/min 140-220C at 15C/min 220C for 3 min 5

Entech 7100 cryogenic sample preconcentrator 400 cc 10 ppb Sulfurs GC/MS 6890/5973 N Scan 29-180 amu 0-6 min 33-280 amu 6-30 min Electron Impact 70 eV

Agilent wishes to thank Entech Instruments for providing this chromatogram.

1. 2. 3. 4. 5. 6. 7. 8. 9.

Hydrogen Sulfide Carbonyl Sulfide Methyl Mercaptan Dimethyl Sulfide Carbon Disulfude Bromochloromethane 1,4-Difluorobenzene Chlorobenzene-d5 4-Bromofluorobenzene

Agilent wishes to thank Entech Instruments for providing this chromatogram.


8 9

2 7

6 4 2 5 1

GC and GC/MS

3.5
C1078

4.0

4.5

5.0

5.5 6.0 6.5 Time (min)

7.0

7.5

8.0

8.5
C1087

10

12 14 Time (min)

16

18

20

C1 and C2 Halocarbons (Freons)


Column:
P/N: Carrier: Oven:

GS-GasPro

Injector:

Detector:

60 m x 0.32 mm id 113-4362 Helium at 35 cm/sec constant velocity 40C for 2 min, 40-120C at 10/min 120C for 3 min 120-200C at 10/min Splitless, 250C 0.20 min purge activation time, 1.0 L of 100 ppm mixture of Accustandard M-REF & M-REF-X in methanol MSD, 280C, full scan 45-180 amu

Compound 1. Chlorotrifluoromethane (peak not shown) 2. Trifluoromethane 3. Bromotrifluoromethane 4. Chloropentafluoroethane 5. Pentafluoroethane 6. 1,1,1-Trifluoroethane 7. Dichlorodifluoromethane 8. Chlorodifluoromethane 9. 1,1,1,2-Tetrafluoroethane 10. Chloromethane 11. 1,1,2,2-Tetrafluoroethane 12. Bromochlorodifluoromethane

Freon # 13 23 13B1 115 125 143a 12 22 134a 40 134 12B1

Compound 13. 1,1-Difluoroethane 14. 1,2-Dichloro-1,1,2,2-tetrafluoroethane 15. 2-Chloro-1,1,1,2-tetrafluoroethane 16. 1-Chloro-1,1-difluoroethane 17. Dichlorofluoromethane 18. Trichlorofluoromethane 19. Chloroethane 20. Dichloromethane 21. 1,1-Dichloro-1-fluoroethane 22. 2,2-Dichloro-1,1,1-trifluoroethane 23. 1,1,2-Trichloro-1,2,2-trifluoroethane 24. 1,2-Dibromo-1,1,2,2-tetrafluoroethane

Freon # 152a 114 124 142b 21 11 160 141b 123 113 114B2

C664

380

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GC and GC/MS Applications


Food and Flavors

Food and Flavors


Fragrance Reference Standard
Column:
P/N: Carrier: Oven: Injector: Detector:

DB-1
30 m x 0.25 mm id, 0.25 m 122-1032 Helium at 25 cm/sec, measured at 150C 40C for 1 min 40-290C at 5/min Split 1:50, 250C 1 L of a 1:20 dilution of neat sample in acetone MSD, 300C transfer line

Many thanks to Carl Frey, Manager of Analytical Services, Dragoco, and Kevin Myung, Director of Flavor and Perfumery Research, Bush Boake Allen, Inc. for contributing to this work.

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 26a. 26b. 26c. 28. 29. 30. 31. 32. 33. 34. 35. 37. 38. 39. 41. 42.

Acetone 2,3-Butanedione (diacetyl) Ethyl acetate 2,3-Pentanedione (acetyl propionyl) Ethyl propionate Methyl butyrate 3-Methylbutyl alcohol 2-Methylbutyl alcohol Isobutyl acetate Ethyl butyrate Furfural Ethyl isovalerate Hexanol Allyl butyrate Ethyl pentanoate Hexylene glycol -Thujone Benzaldehyde -Pinene Camphene 3,5,5-Trimethylhexanol Sabinene -Pinene Ethyl hexanoate Myrcene Hexyl acetate cis-Linalool oxide Methyl benzoate trans-Linalool oxid Methyl-cresol Benzyl alcohol para-Cymene 1,8-Cineol Limonene 2,6-Dimethylhept-5-enal -Terpinene Octanol Ethyl heptanoate Linalool Benzene ethanol Rose oxide, cis-rose Rose oxide, trans-rose

43. 44. 45. 46. 47. 48. 49. 51. 52. 53. 54. 56. 57. 58. 59. 59a. 60. 61. 62. 63. 64. 66. 67. 68. 69. 70. 71. 72. 73. 74. 76. 78. 79. 80. 81. 82. 83. 85. 88. 89. 90. 91.

Camphor Citronellal Benzyl acetate Menthone Isoborneol Isomenthone Borneol Terpinen-4-ol -Terpineol Ethyl octanoate Octyl acetate Fenchyl acetate Citronellol Neral Carvonel Phenylethyl acetate Geraniol Linalyl acetate Geranial Hydroxycitronellal Citronellyl formate Bornyl acetate Vertenex (isomer 1) Ethyl nonanoate Geranyl formate Vertenex (isomer 2) -Nonalactone Citronellyl acetate Neryl acetate Geranyl acetate Diphenyl oxide Ethyl decanoate -Copaene Florazone (isomer 1) Florazone (isomer 2) -Caryophyllene Citronellyl propionate 3,7-Guaiadiene Dodecanol Ethyl undecanoate Eugenyl acetate Frambione (raspberry ketone)

93. Isoamyl salicylate 94. -Cadinene 95. cis-Nerolidol 96. Rosatol (rosetone) 96a. Geranyl butyrate 97. trans-Nerolidol 98. n-Amyl salicylate 99. Phenylethyl tiglate 100. Ethyl dodecanoate 101. Benzophenone 102. Dibenzyl ether 103. -Dodecalactone 104. Citronellyl tiglate 105. Evernyl 106. Geranyl tiglate 107. Geranyl-2-methyl valerate 108. Celestocide 109. Heptadec-1-ene 110. Benzyl benzoate 111. Ethyl tetradecanoate 112. Benzyl salicylate 113. Tonalid 114. Nonadec-1-ene 115. Isopropylmyristate 116. Ethyl pentadecanoate 116a. Nonadecane 117. Ethyl hexadecanoate 118. Musk T (ethylene brassylate) 119. Eicosane 120. Cinnamyl phenyl acetate 121. Heneicosane 122. Phenyl ethyl cinnamate 123. Ethyl octadecanoate 124. Hercolyn D (tetrahydro & dihydro methyl abietate) 125. Cinnamyl cinnamate 126. Cetearyl octanoate 127. Cetearyl decanoate

GC and GC/MS

C389

www.agilent.com/chem

381

GC and GC/MS Applications


Food and Flavors
Fragrance Reference Standard
Column:
P/N: Carrier: Oven: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38. 39. Acetone Ethyl acetate Ethyl propionate 2,3-Butanedione (diacetyl) Methyl butyrate Isobutyl acetate -Pinene Ethyl butyrate 2,3-Pentanedione (acetyl propionyl) Camphene Ethyl isovalerate -Pinene Ethyl pentanoate Myrcene Allyl butyrate Limonene 1,8-Cineol 3,5,5-Trimethylhexanol 3-Methylbutyl alcohol 2-Methylbutyl alcohol Ethyl hexanoate -Terpinene p-Cymene Hexyl acetate Terpinolene Ethyl heptanoate 2,6-Dimethylhept-5-enal (MelonalTM) Rose oxide, cis-rose Hexanol Rose oxide, trans-rose Methyl-para-cresol Ethyl octanoate cis-Linalool oxide Menthone Furfural trans-Linalool oxide Octyl acetate Isomenthone -Copaene 40. 41. 42. 43. 44. 45. 46. 47. 48. 49. 50. 51. 52. 53. 54. 55. 56. 57. 58. 59. 60. 61. 62. 63. 64. 65. 66. 67. 68. 69. 70. 71. 72. 73. 74. 75. 76. 77. 78. Camphor Benzaldehyde Ethyl nonanoate Linalool Linalyl acetate Vertenex (isomer 1) Octanol -Caryophyllene Vertenex (isomer 2) Terpinen-4-ol Methyl benzoate Hexylene glycol Ethyl decanoate Citronellyl acetate Isoborneol Neral -Terpineol Geranyl formate Borneol -Bisabolene Benzyl acetate Neryl acetate Geranial Ethyl undecanoate -Cadinene Geranyl acetate Citronellol Ethyl dodecanoate Geraniol Benzyl alcohol Geranyl butyrate Nonadecane Benzene ethanol Nonadec-1-ene Florazone (isomer 1) Florazone (isomer 2) Hydroxycitronellal Dodecanol Diphenyl oxide 79. 80. 81. 83. 84. 85. 86. 87. 90. 91. 92. 93. 94. 95. 96. 97. 98. 99. 100. 101. 102. 103. 104. 105. 106. Citronellyl tiglate Eugenyl methyl ether -Nonalactone Ethyl tetradecanoate n-Amyl salicylate Geranyl tiglate Ethyl pentadecanoate Isopropylmyristate Phenylethyl tiglate Rosatol (rosetone) Eugenyl acetate Ethyl hexadecanoate -Dodecalactone Dibenzyl ether Tonalid Ethyl octadecanoate Benzophenone Benzyl benzoate Cetearyl octanoate Musk T (ethylene brassylate) Cetearyl decanoate Frambione (raspberry ketone) Cinnamyl phenyl acetate Phenyl ethyl cinnamate Cinnamyl cinnamate

DB-WAX
30 m x 0.25 mm id, 0.25 m 122-7032 Helium at 25 cm/sec, measured at 150C 45C for 2 min 45-250C at 3/min 250C for 34 min Split 1:50, 250C 1 L of a 1:20 dilution of neat sample in acetone MSD, 250C transfer line

Injector:

Detector:

Many thanks to Carl Frey, Manager of Analytical Services, Dragoco, and Kevin Myung, Director of Flavor and Perfumery Research, Bush Boake Allen, Inc. for contributing to this work.

GC and GC/MS
C390

382

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GC and GC/MS Applications


Food and Flavors
Perfume
Column: HP-INNOWax
P/N: Carrier : Oven: 30 m x 0.25 mm id, 0.25 m 19091N-133 Helium, 30 cm/sec 0.9 mL/min constant flow 80C for 1 min 80-250C at 5C/min 250C for 2 min Split 10:1, 250C MSD 280C

Flavor Mixture
Column: Ultra 2
P/N: Carrier: Injector: Oven: 25 m x 0.32 mm id, 0.52 m 19091B-112 Helium, 90 kPa., 2.2 mL/min constant flow Split 20:1, 250C 80C for 1 min 80-210C at 8C/min 210C for 2 min IRD, 280C, Wide Band MCT, 550 to 4000 cm-1

Injector: Detector:

1. 2. 3. 4. 5. 6. 7. 8. 9. 10.

Limonene Linalool Linalyl acetate Benzyl acetate Citronellol Benzene ethanol -Methyl Ionone Carvocrol and geraiol Isoamyl salicylate n-Amyl salicylate

11. 12. 13. 14. 15. 16. 17. 18. 19.

Commamyl acetate Acetylcedrene Diethyl phthalate Tonalid Coumarin Musk xylene Benzyl benzoate Benzyl salicylate Musk ketone

Detector:

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13.

Fenchone Thujone Benzaldehyde trans-Carveol Farnesol cis-Carveol trans-Geraniol Citral Eugenol Vanillan trans-Isoeugenol trans-Citronellyl tiglate cis-Citronellyl tiglate

13 9 18 6 4 3 2 1 5 7 11 8 10 2 12

15 12 14

16 17 1 4 3 19 5 7 68 11 0 C1060 5 10 Time (min) 15 10

GC and GC/MS

C1056

10

15

20 Time (min)

25

30

Separation of Chiral Compounds Commonly Found in Essential Oils and Fragrances


Column:
P/N: GC: Carrier: Oven: Injection: Sample: Detection:

Menthol
Column:
1,2-Dimethylbenzene Myrcene (-)-Camphene (+)-Camphene (+)--pinene 1S-(-)--pinene Cineole (R)-(+)-Citronellal 1S,2R,5S-(+)-Menthol 1R,2S,5R-(-)-Menthol -Terpineol (+/-)-isoborneol (+)-borneol trans-Cinnamaldehyde P/N: Carrier: Oven: Injector: Detector:

Cyclodex-B
30 m x 0.25 mm id, 0.25 m, 112-2532 Hydrogen, 55 cm/sec 105 C isothermal Split ratio 1:100, 250C 1 L of 1 g/L each chloroform FID, 300C Nitrogen makeup gas at 30 mL/min

HP-Chiral-20 b
30 m x 0.25 mm id, 0.25 m, 19091G-B233 6890 Hydrogen, 39 cm/sec, Constant pressure 65 C for 1 min 65-170 C at 5 C/min Split ratio 30:1, 1 L, 250C 0.25 ng/L each analyte in Hexane FID, 300C 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14.

1. 2. 3. 4.

(+)-Neomenthol (-)- Neomenthol (+)-Menthol (-)-Menthol

14

7 5 6

9 10 13 11 12

4 3

C1079

10 Time (min)

15

20

C082

www.agilent.com/chem

383

GC and GC/MS Applications


Food and Flavors
Garlic Oil
Column:
P/N: Guard Column: Carrier : Oven: 1. 2. 3. 4. 5. 6. 7. 8. 9. Allymethylsulfide Methyldisulfide Allylsulfide Allylmethyldisulfide cis-1-(Methyldithio)-1-propene Methylpropyldisulfidde l trans-1-(Methyldithio)-1-propene Dimethyltrisulfide Dipropenyldisulfide 10. 11. 12. 13. 14. 15. 16. 17. 18. Allylpropenyldisulfide Allyldisulfide Dipropyldisulfide Allylmethyltrisulfide Methylpropyltrisulfide 3-Vinyl-1,2-dithiin 2-Vinyl-1,3-dithiin Dimethyltetrausulfide Allyltrisulfide

DB-XLB
30 m x 0.25 mm id, 0.5 m 122-1236 1 m x 0.53 mm id deactivated tubing Helium at 41 cm/sec, measured at 45C 45C for 3 min 45-240C at 5/min 240C for 3 min Hot On-column, 100C 108 mL/min split vent flow 1.2 L dichloromethane plug with 1.2 L of 1.0 mg/mL sample of garlic oil in dichloromethane MSD, 200C transfer line full scan 35-215 amu

Injector:

Detector:

GC and GC/MS

C461

Lemon Oil
Column:
P/N: Carrier : Oven:

DB-5
20 m x 0.10 mm id, 0.1 m 127-5022 Hydrogen at 60 cm/sec, measured at 40C 40C for 3 min 40-185C at 30/min 185C for 3 min Split 1:275, 275C Nitrogen makeup gas at 30 mL/min

Injector: Detector:

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14.

-Thujone -Thujone Camphene Sabinene -Pinene Myrcene Octanal -Phellandrene -Terpinene r-Cymene -Limonene -Trepinene Octanol Terpinolene

15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28.

Linalool Nonanal Citronellal Trepinen-4-ol -Trepineol Decanal Neral Geranial Nerylacetate Geranylacetate -Caryophyllene trans--Bergamotene -Humulene -Bisabolene

Faster Analysis Time With 0.1 mm ID Columns!

C519

384

www.agilent.com/chem

GC and GC/MS Applications


Food and Flavors
Cold-Pressed Orange Oil
Column:
P/N: Carrier : Oven: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. -Pinene Sabinene Myrcene Octanal Limonen Linalool Nonanal Citonellal -Terpineol Decanal 11. 12. 13. 14. 15. 16. 17. 18. 19. Neral Geranial Dodecenal Valencene Cadinene -Sinensal -Sinensal Nootkatone Polymethoxyflavones

DB-5
20 m x 0.1 mm id, 0.1 m 127-5022 Hydrogen at 60 cm/sec, measured at 70C 70C for 1 min 70-250C at 30/min 250-310C at 20/min 310C for 2 min Split 1:275, 275C FID, 350C Nitrogen makeup gas at 30 mL/min

Injector: Detector:

Chromatogram courtesy of Tastemaker

Faster Analysis Time With 0.1 mm ID Columns!

GC and GC/MS

C521

Peppermint Oil
Column:
P/N: Carrier : Oven:

DB-WAX
60 m x 0.25 mm id, 0.25 m 122-7062 Helium at 25 cm/sec (0.73 mL/min) 75C for 8 min 75-200C at 4/min 200C for 5 min Split 1:150, 270C 1L neat FID, 270C Nitrogen makeup gas at 30 mL/min

Injector: Detector:

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15.

-Pinene -Pinene Sabinene Myrcene -Terpinene /-Limonene 1,8-Cineol cis-Ocimene Terpinene r -Cymene -Terpinolene 3-Octanol 1-Octen-3-ol trans-Sabiene hydrate /-Methone

16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29.

Methofuran d-Isomethone -Bourbonene Linalool Methylacetate Neomenthol Trepinen-4-ol -Caryophyllene /-Menthol Pulegone -Terpineol Germacrene-D Piperitone Viridiflorol

Thanks to: Mr. William Faas of A.M. Todd Company for providing the sample and assisting with peak identification.
C210

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385

GC and GC/MS Applications


Food and Flavors
Spearmint Oil (Western)
Column:
P/N: Carrier : Oven:

DB-WAX
60 m x 0.25 mm id, 0.25 m 122-7062 Helium at 25 cm/sec (0.73 mL/min) 75C for 8 min 75-200C at 4/min 200C for 5 min Split 1:150, 270C 1 L neat FID, 270C Nitrogen makeup gas at 30 mL/min

Injector: Detector:

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15.

-Pinene -Pinene Sabinene Myrcene -Terpinene /-Limonene 1,8-Cineol cis-Ocimene -Terpinene r-Cymene Terpinolene 3-Octylacetate 3-Octanol trans-Sabiene hydrate /-Methone

16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30.

-Bourbonene Linalool Trepinen-4-ol -Caryophyllene Dihydrocarvone trans-Dihydrocarvyl trans- -Farnesene -Terpineol Germacrene-D /-Carvone cis-Carvylacetate trans-Carveol cis-Carveol cis-Jasmone Viridiflorol

Thanks to: Mr. William Faas of A.M. Todd Company for providing the sample and assisting with peak identification.

GC and GC/MS

C209

Ylang Ylang Oil


Column:
P/N: Carrier : Oven: Injector: Detector:

DB-XLB
30 m x 0.25 mm id, 0.25 m 122-1232 Helium at 34 cm/sec, measured at 50C 50C for 1 min 50-250C at 3.5/min Split 1:125, 250C 1L of 10% oil in methylenechloride MSD, full scan at m/z 35-550 310C transfer line

1. r-Methylansiole 2. Linalool 3. Methylbenzoate 4. Benzylacetate 5. Geraniol 6. Geranylacetate 7. -Caryophyllene 8. Cinnamylacetate 9. Germacrene-D 10. Benzylbnzoate 11. Faneosolacetate 12. Benzylsalicylate

C577

386

www.agilent.com/chem

GC and GC/MS Applications


Food and Flavors
Rosemary Oil
Column:
P/N: Carrier : Oven: Injector: Detector:

CycloSil-B
30 m x 0.25 mm id, 0.25 m 112-6632 Hydrogen at 40 cm/sec, measured at 60C 55C for 1 min 50-180C at 5/min Split 50:1, 250C FID, 340C 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. (+/-) -Pinene (+/-) Camphene (+/-) -Pinene (+/-) Limonene (+/-) Linalool (+/-) Camphor (+/-) Terpinene-4-ol (+/-) Isoborneol (+/-) Borneol (+/-) -Terpineol

GC and GC/MS

C509

Citrus Flavored Carbonated Beverage (Soda)


Column:
P/N: Carrier : Oven: Detector: SPME Fiber: Extraction:

CycloSil-B
30 m x 0.25 mm id, 0.25 m 112-6632 Helium at 37 cm/sec, measured at 40C 40-190C at 2 min HP5973MSD, 280C transfer line Ployacrylate fiber, 85 m Headspace, no stir, NaCl 1g/10 mL sample 68 min at 27C Adsorption 250C for 15 min, Split 1:5

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11 12 13

S(-)Limonene p-Cymene R(+)Limonene Octanol -Terpinene Nonanol 2-Ethyl-1-Hexanol Linalool Decanol Terpinen-4-ol Phenethylalcohol -Terpineol BHT

Desorption

C740

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387

GC and GC/MS Applications


Food and Flavors
Alcohol Beverage Standard
Column: HP-FFAP
P/N: Carrier: Injection: Oven: 50 m x 0.2 mm id, 0.33 m 19091F-105 Hydrogen Split, 100:1, 1 L 60C for 4 min 60-200C at 6C/min 200C for 2 min FID 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. Acetaldehyde Acetone Ethyl formate Ethyl acetate Acetal Methanol Ethanol Diacetyl sec-butanol n-propanol Isobutanol n-butanol Isoamyl alcohol n-amyl alcohol Acetic acid Propionic acid

Bourbon
Column: HP-INNOWax
P/N: Carrier: Oven: 30 m x 0.25 mm id, 0.25 m 19091N-133 Helium, 33 cm/sec, 15.5 psi (35C) 1.5 mL/min constant flow 35C for 5 min 35-150C at 5C/min 150-250C at 20C/min 250C for 2 min Split 25:1, 220C,1 L FID 280C

Detector:

Injection: Detector:

1. 2. 3. 4. 5. 6. 7. 8.

Acetaldehyde Ethyl acetate Methanol Ethanol Acetic acid n-Propanol Isobutanol 2-Methyl-1-butanol or 3-Methyl-1-Butanol

11 10 9 2 5 4 2 3 8 7 13 14 15 1 3 5 12 8

GC and GC/MS

C1050

20 min

C1052

10 Time (min)

20

30

Alditol Acetates
Column: DB-225
P/N: Carrier: Oven: Injector: Detector: 30 m x 0.25 mm id, 0.15 m 122-2231 Hydrogen a 36.5 cm/sec 220C isothermal Split, 1:50, 1 L, 225C FID, 250C Nitrogen makeup gas at 30 mL/min

Strawberry Syrup
Column: HP-INNOWax
P/N: Carrier: Oven: 30 m x 0.32 mm id, 0.5 m 19091N-213 Helium, 40 cm/sec, 11.7 psi (60C) 2.5 mL/min constant flow 60C for 1 min 60-250C at 10C/min 250C for 2 min Split 60:1, 220C FID 275C

Injector: Detector: 1. 2. 3. 4. 5. 6. 7. 8. Rhamnitol Fucitol Ribitol Arabinitol Mannitol Galactitol Glucitol Inositol

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12.

Ethyl acetate Ethyl butyrate Isoamyl acetate Amyl acetate Isoamyl butyrate Amyl butyrate Ethyl benzoate Citronellol Geraniol Ethyl-3-phenyl oxiran carboxylate Strawberry aldehyde Benzyl benzoate

2 3 4

11

10

5 7 8 9 12

0 C085 C1062

10 Time (min)

15

20

388

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GC and GC/MS Applications


Food and Flavors
Sulfur and Selenium in Garlic by Headspace
Column:
P/N: Carrier:

HP-INNOWax
60 m x 0.32 mm id, 0.25 m 19091N-116 (A): Helium, 28 cm/sec, 18 psi (35C), 1.2 mL/min constant flow (B): Helium, 28 cm/sec, 5.6 psi (35C), 1.2 mL/min constant flow 35C for 3 min 35-230C at 5C/min 230C for 5 min Splitless, SI inlet (140C), AED 190C and MSD 230C

Oven:

Injector: Detector:

1. CH3-Se-CH3 2. C3H5-S-CH3 3. C3H5-Se-CH3 4. CH3-S-S-CH3 5. C3H5-S-C3H5 6. CH3-S-Se-CH3 7. C3H5-S-S-CH3 7a. CH3-Se-Se-CH3

8. 9. 10. 11. 12. 13. 14.

CH3-Se-S-C3H5 CH3-S-S-S-CH3 C3H5-S-S-C3H5 CH3-S-Se-S-CH3 CH3-S-S-S-C3H5 C3H5-S-S-S-C3H5 C3H5-S-S-S-C3H5

(A) AED

(B) MSD
5 2 10

10 2 4 5 6 8 7 9

12

13 7 12 13

1 3 1 0
C1069

4 3 6 10 89 11 25

7a 10 Time (min) 20

11 30
C1070

GC and GC/MS

15 20 Time (min)

Free Fatty Acids


Column: HP-INNOWax
30 m x 0.25 mm id, 0.25m 19091N-133 Helium 42 cm/sec, 24 psi (120C) 1.8 mL/min constant flow 120C for 1 min 120-250C at 10C/min 250C for 5 min Split 40:1, 250C, 1 L 0.05 to 0.11% each in methylene chloride FID 280C

TMS Sugars
Column:
P/N: Carrier: Oven: Injector: Detector:

DB-1701
30 m x 0.25 mm id, 0.25 m 122-0732 Hydrogen at 40 cm/sec 180C for 2 min 180-200C at 5/min Split 1:50, 225C FID, 250C Nitrogen makeup gas at 30 mL/min

P/N: Carrier: Oven:

Injection: Sample: Detector:

1. 2. 3. 4. 5. 6. 7. 8. 9.

D-Arabinose D-Ribose anomers D-Zylose D-Mannose D-Fructose -D-Galactose -D-Glucose D-Fructose (anomer) -D-Glucose

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18.

C2 C3 C4 C5 C5-Iso C6 C7 C8 C10 C12 C14 C14:1 C16 C16:1 C18 C18:1 C18:2 C18:3

1 9 8 34 5 6 7 10 11

14 16

12 13 15 17 18

C086

0
C1047

15 Time (min)

www.agilent.com/chem

389

GC and GC/MS Applications


Food and Flavors
Organic Acids
Column:
P/N: Carrier: Oven:

DB-FFAP
30 m x 0.25 mm id, 0.25 m 122-3232 Helium at 40 cm/sec, measured at 100C 100C for 5 min 100-250C at 10/min 250C for 12 min Split 1:50, 250C FID, 300C Nitrogen makeup gas at 30 mL/min

Injector: Detector:

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11.

Acetone Formic acid Acetic acid Propionic acid Isobutyric acid Butyric acid Isovaleric acid Valeric acid (pentanoic acid) Isocaproic acid Caproic acid (hexanoic acid) Heptanoic acid

12. 13. 14. 15. 16. 17. 18.

Octanoic acid Decanoic acid Dodecanoic acid Tetradecanoic acid Hexadecanoic acid Octadecanoic acid Arachidic acid (eicosanoic acid)

GC and GC/MS

C077

Bacterial Fatty Acid Methyl Esters


Column:
P/N: Carrier: Oven: Injector: Detector:

DB-5
30 m x 0.25 mm id, 0.25 m 122-5032 Hydrogen at 42 cm/sec 150C for 4 min 150-250C at 4/min Split 1:100 FID Nitrogen makeup gas at 30 mL/min Methylundecanoate Methyl 2-hydroxydecanoate Methyl laurate Methyl tridecanoate Methyl 2-hydroxydodecanoate Methyl 3-hydroxydodecanoate Methyl myristate Methyl 12-methyltetradecanoate Methyl pentadecanoate Methyl 2-hydroxytetradecanoate Methyl 3-hydroxytetradecanoate Methyl palmitoleate Methyl palmitate Methyl 14-methylhexadecanoate Methyl cis-9,10-methyl hexadecanoate Methyl heptadecanoate Methyl 2-hydroxyhexadecanoate Methyl oleate Methyl elaidate Methyl stearate Methyl cis-9,10-methylene octadecanoate Methyl nonadecanoate Methyl arachidate

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21.

C11:0 2-OH C10:0 C12:0 C13:0 2-OH C12:0 3-OH C12:0 C14:0 12-Me C14:0 C15:0 2-OH C14:0 3-OH C14:0 C16:1 C16:0 14-Me C16:0 9,10-diMe C16:0 C17:0 2-OH C16:0 C18:1 C18:1 C18:0 9,10-diMe C18:0

22. C19:0 23. C20:0

C068

390

www.agilent.com/chem

GC and GC/MS Applications


Food and Flavors
FAMES
Column:
P/N: Carrier: Hydrogen at 43 cm/sec Constant pressure mode 130C for 1.0 min 130-170C at 6.5C/min 170-215C at 2.75C/min 215C for 12 min 215-230C at 40C/min 230C for 3 min Split, 270C Split ratio 50:1 FID, 280C 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. C6:0 C7:0 C8:0 C9:0 C10:0 C11:0 C12:0 BHT C13:0 C14:0 C14:1n5 C15:0 C16:0 C16:1n7(trans) C16:1n7(cis) C17:0 C17:1 C18:0 C18:1n9(trans) C18:1n9(cis) C18:1n7 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38. 39. 40. 41. C18:2n6 C18:3n6 C18:3n3 C18:2(d9,11) C18:2(d10,12) C20:0 C20:1n9 C20:2n6 C20:3n6 C20:4n6 C20:3n3 C20:5n3 C22:0 C22:1n9 C22:2n6 C22:4n6 C22:5n3 C24:0 C22:6n3 C24:1n9

DB-23
60 m x 0.25 mm id, 0.25 m 122-2362

Oven:

6 9 8 5 3 4 1 2 10 11 13 15 16 17 14 7 12

Injector: Detector:

Baseline Resolution of C16 and C18 cis-, trans- isomers

20

19 21

18

22

23 24

2526 27

28

29 30

3132

Short Analysis Time 33 34 35 36 37 383940 41

GC and GC/MS

2
C796

10

12

14 Time (min)

16

18

20

22

24

26

28

Chromatogram provided courtesy of Steve Watkins and Jeremy Ching, FAME Analytics, http://www.fameanalytics.com

Polyunsaturated Fatty Acid Methyl Esters


Column:
P/N: Sample: Carrier: Oven:

HP-INNOWax
30 m x 0.32 mm id, 0.5 m 19091N-213 50 g/L in methylene chloride Helium, 30 cm/sec, 10.7 psi (150C) 1.5 mL/min constant flow 150C for 1 min 150-200C at 15C/min 200-250C at 2C/min 250C for 5 min Split (60:1), 0.5 L, inlet 220C FID, 275C 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. C14:0 C16:0 C16:1w7 C18:0 C18:1w9 C18:1w7 C18:2w6 C18:3w3 C20:1w9 C20:2w6 C20:3w6 C20:4w6 C20:5w3 C22:4w6 C22:5w3 C22:6w3

Can't find what you're looking for?


Save time and avoid trial and error by accessing pre-defined, high-quality applications. Consult Agilent's online, searchable Chromatogram Database
Containing hundreds of commonly analyzed compounds, this resource helps chromatographers select the right analytical technique, column and conditions for an application. Search the extensive database for GC, LC and CE applications by compound name or CAS number.

Injection: Detector:

4 6 5 2

12 14

7 3 8 1 9 10 11 13 15 16

To access the Chromatogram Database go to www.agilent.com/chem and click on "Library."

0
C1045

10

15 Time (min)

20

25

www.agilent.com/chem

391

GC and GC/MS Applications


Food and Flavors
FAME Standard
Column:
P/N: Carrier: Oven: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. Butyric acid methyl ester (C4:0) Caproic acid methyl ester (C6:0) Caprylic acid methyl ester (C8:0) Capric acid methyl ester (C10:0) Undecanoic acid methyl ester (C11:0) Lauric acid methyl ester (C12:0) Tridecanoic acid methyl ester (C13:0) Myristic acid methyl ester (C14:0) Myristoleic acid methyl ester (C14:1) Pentadecanoic acid methyl ester (C15:0) cis-10-Pentadecenoic acid methyl ester (C15:1) Palmitic acid methyl ester (C16:0) Palmitoleic acid methyl ester (C16:1) 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. Heptadecanoic acid methyl ester (C17:0) cis-10-Heptadecenoic acid methyl ester (C17:1) Stearic acid methyl ester (C18:0) Oleic acid methyl ester (C18:1n9c) Elaidic acid methyl ester (C18:1n9t) Linoleic acid methyl ester (C18:2n6c) Linolelaidic acid methyl ester (C18:2n6t) -Linolenic acid methyl ester (C18:3n6) Linolenic acid methyl ester (C18:3n3) Arachidic acid methyl ester (C20:0) cis-11-Eicosenoic acid methyl ester (C20:1) cis-11,14-Eicosadienoic acid methyl ester (C20:2) cis-8,11,14-Eicosatrienoic acid methyl ester (C20:3n6) Heneicosanoic acid methyl ester (C21:0) cis-11,14,17-Eicosatrienoic acid methyl ester (C20:3n3) Arachidonic acid methyl ester (C20:4n6) cis-5,8,11,14,17-Eicosapentaenoic acid methyl ester (C20:5n3) Behenic acid methyl ester (C22:0) Erucic acid methyl ester (C22:1n9) cis-13,16-Docosadienoic acid methyl ester (C22:2) Tricosanoic acid methyl ester (C23:0) Lignoceric acid methyl ester (C24:0) cis-4,7,10,13,16,19-Docosahexaenoic acid methyl ester (C22:6n3) Nervonic acid methyl ester (C24:1)

DB-WAX
10 m x 0.10 mm id, 0.10 m 127-7012 Hydrogen at 77 cm/sec, measured at 40C 40C for 0.5 min 40-195C at 25/min 195-205C at 3/min 205-230C at 8/min 230C for 1 min Split 1:30, 250C FID, 250C

Injector: Detector:

Excellent Resolution in 12 Minutes!

GC and GC/MS

C583

FAME Standard
Column:
P/N: Carrier: Oven:

DB-225
20 m x 0.10 mm id, 0.10 m 127-2222 (cut to 10 m) Hydrogen at 59.3 cm/sec, measured at 35C 35 for 0.5 min 35-195C at 25/min 195-205C at 3/min 205-230C at 8/min 230C for 1 min Split 1:30, 250C FID, 250C

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13.

Butyric acid methyl ester (C4:0) Caproic acid methyl ester (C6:0) Caprylic acid methyl ester (C8:0) Capric acid methyl ester (C10:0) Undecanoic acid methyl ester (C11:0) Lauric acid methyl ester (C12:0) Tridecanoic acid methyl ester (C13:0) Myristic acid methyl ester (C14:0) Myristoleic acid methyl ester (C14:1) Pentadecanoic acid methyl ester (C15:0) cis-10-Pentadecenoic acid methyl ester (C15:1) Palmitic acid methyl ester (C16:0) Palmitoleic acid methyl ester (C16:1)

Injector: Detector:

14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33. 34. 35. 36.

Heptadecanoic acid methyl ester (C17:0) cis-10-Heptadecenoic acid methyl ester (C17:1) Stearic acid methyl ester (C18:0) Oleic acid methyl ester (C18:1n9c) Elaidic acid methyl ester (C18:1n9t) Linoleic acid methyl ester (C18:2n6c) Linolelaidic acid methyl ester (C18:2n6t) -Linolenic acid methyl ester (C18:3n6) Linolenic acid methyl ester (C18:3n3) Arachidic acid methyl ester (C20:0) cis-11-Eicosenoic acid methyl ester (C20:1) cis-11,14-Eicosadienoic acid methyl ester (C20:2) cis-8,11,14-Eicosatrienoic acid methyl ester (C20:3n6) Heneicosanoic acid methyl ester (C21:0) cis-11,14,17-Eicosatrienoic acid methyl ester (C20:3n3) Arachidonic acid methyl ester (C20:4n6) cis-5,8,11,14,17-Eicosapentaenoic acid methyl ester(C20:5n3) Behenic acid methyl ester (C22:0) Erucic acid methyl ester (C22:1n9) cis-13,16-Docosadienoic acid methyl ester (C22:2) Tricosanoic acid methyl ester (C23:0) Lignoceric acid methyl ester (C24:0) cis-4,7,10,13,16,19-Docosahexaenoic acid methyl ester (C22:6n3) 37. Nervonic acid methyl ester (C24:1)

Faster Analysis Time With 0.10 mm ID Columns!

C582

392

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GC and GC/MS Applications


Food and Flavors
Canola Oil Margarine Partially Hydrogenated FAMEs AOCS Method 1c-89
Column:
P/N: Carrier: Oven: Injector: Detector: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10.

DB-23
60 m x 0.25 mm id, 0.25 m 122-2362 Helium at 15 cm/sec (0.44 mL/min), measured at 150C 150-200C at 1.3/min 200C for 10 min Split 1:100, 1 L, 210C FID, 210C

C16:0 Methyl palmitate C16:1 Methyl palmitoleate C18:0 Methyl stearate C18:1 trans-Methyl elaidate and Multiple isomers C18:1 cis-Methyl oleate and Multiple isomers C18:2 trans-Multiple isomers C18:2 cis-Multiple isomers C18:3 Methyl linolenate C20:0 Methyl arachidate C20:1 Methyl 11-eicosanoate

C211

GC and GC/MS

Butter Triglycerides
Column:
P/N: Carrier: Oven:

Butter Triglycerides
Column:
P/N: Carrier: Oven: Injector:

DB-5ht
30 m x 0.32 mm id, 0.1 m 123-5731 Hydrogen at 55 cm/sec, measured at 250C 35-250C at 70/min 250-400C at 5/min 400C for 20 min Cool On-column 1 L of 9 g/L in toluene (approx. 1% w/w solution) FID, 400C Nitrogen makeup gas at 30 mL/min Baseline Corrected

DB-17ht
30 m x 0.32 mm id, 0.15 m 123-1831 Hydrogen at 40 cm/sec 250-365C at 5/min 365C for 1 min Cool On-column 1 L of 9 g/L in toluene (approx. 1% w/w solution) FID, 400C Nitrogen makeup gas at 30 mL/min Baseline Corrected

Injector:

Detector:

Detector:

T= Total number of Carbons

Peak Identification: P=Palmitic acid, hexadecanoic acid, C16:0 S=Stearic acid, octadecanoic acid, C18:0 O=Oleic acid, cis-9-octadecanoic acid, C18:1

C079

C212

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393

GC and GC/MS Applications


Industrial Chemicals

Industrial Chemicals
Alcohols
Column:
P/N: Carrier: Oven:

DB-624
-Prosteel version 30 m x 0.53 mm id, 3.0 m 125-1334 Helium at 30 cm/sec, measured at 40C 40C for 5 min 40-260C at 10C/min 260C for 3 min Split 1:10, 250C 1 L of 0.01-0.05% each solvent in CS2 FID, 300C Nitrogen makeup gas at 30 mL/min
3, CS2 7,8 1

Injector: Detector:

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13.

Methanol Ethanol Isopropanol tert-Butanol 2-Propen-1-ol (allyl alcohol) 1-Propanol 2-Propyn-1-ol (propargyl alcohol) sec-Butanol 2-Methyl-3-buten-2-ol Isobutanol 2-Methoxyethanol (methyl Cellosolve) 3-Buten-1-ol 2-Methyl-2-butanol (tert-amyl alcohol)

14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26.

1-Butanol 2-Buten-1-ol (crotyl alcohol) Ethylene glycol 1-Penten-3-ol 2-Pentanol Glycidol 3-Pentanol 2-Ethoxyethanol (Cellosolve) Propylene glycol 3-Methyl-1-butanol (isoamyl alcohol) 2-Methyl-1-butanol (active amyl alcohol) 4-Methyl-2-pentanol 1-Pentanol

22,29,30

37,39

41,42,43 44

49,50,52

18,20 28 26,27 24 19 15 17 11 13 12 14 10 9 23 21 16 25 31 34 36 35 32 40 38 33 45 47 46 51 48

27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38. 39. 40. 41. 42. 43. 44. 45. 46. 47. 48. 49. 50. 51. 52.

2-Penten-1-ol 3-Methyl-2-buten-1-ol Cyclopentanol 3-Hexanol 2-Hexanol 4-Hydroxy-4-methyl-2-pentanone Furfuryl alcohol cis-3-Hexen-1-ol 1-Hexanol cis-2-Hexen-1-ol Cyclohexanol 3-Heptanol 2-Heptanol 2-Butoxyethanol (butyl Cellosolve) cis-4-Hepten-1-ol trans-2-Hepten-1-ol 1-Heptanol Benzyl alcohol 2-Ethyl-1-hexanol a-Methylphenyl alcohol 1-Octanol 1-Nonanol 2-Phenoxyethanol a-Ethylphenethyl alcohol b-Ethylphenethyl alcohol 1-Decanol

5 2 4 6

GC and GC/MS

10 Time (min)

15

20

C397

Alcohols
Column:
P/N: Carrier: Oven:

DB-WAXetr
50 m x 0.32 mm id, 1.0 m 123-7354 Helium at 50 cm/sec, measured at 40C 40C for 5 min 40-230C at 10/min 230C for 5 min Split 1:5, 250C 1 L of 0.15% each solvent in CS2 FID, 250C Nitrogen makeup gas at 35 mL/min

Injector:

14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25.

1-Butanol 2-Buten-1-ol (crotyl alcohol) Ethylene glycol 1-Penten-3-ol 2-Pentanol Glycidol 3-Pentanol 2-Ethoxyethanol (Cellosolve) Propylene glycol 3-Methyl-1-butanol (isoamyl alcohol) 2-Methyl-1-butanol (active amyl alcohol) 4-Methyl-2-pentanol

26. 27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37.

1-Pentanol 2-Penten-1-ol 3-Methyl-2-buten-1-ol Cyclopentanol 3-Hexanol 2-Hexanol 4-Hydroxy-4-methyl-2-pentanone Furfuryl alcohol cis-3-Hexen-1-ol cis-2-Hexen-1-ol Cyclohexanol 3-Heptanol

Detector:

38. 39. 40. 41. 42. 43. 44. 45. 46. 47. 48. 49. 50.

2-Heptanol 2-Butoxyethanol (butyl Cellosolve) cis-4-Hepten-1-ol trans-2-Hepten-1-ol 1-Heptanol Benzyl alcohol 2-Ethyl-1-hexanol 1-Octanol 1-Nonanol 2-Phenoxyethanol a-Ethylphenethyl alcohol b-Ethylphenethyl alcohol 1-Decanol

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13.

Methanol Ethanol Isopropanol tert-Butanol 2-Propen-1-ol (allyl alcohol) 1-Propanol 2-Propyn-1-ol (propargyl alcohol) sec-Butanol 2-Methyl-3-buten-2-ol Isobutanol 2-Methoxyethanol (methyl Cellosolve) 3-Buten-1-ol 2-Methyl-2-butanol (tert-amyl alcohol)

C550

394

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GC and GC/MS Applications


Industrial Chemicals
Alcohols
Column:
P/N: Carrier: Oven:

Ethoxyethanol
HP-INNOWax
30 m x 0.53 mm id, 1.0 m 19095N-123 Helium, 29 cm/sec, 3.0 psi (45C) 45C for 1 min 45-150C at 10C/min 4 mL/min constant flow Split (25:1), 250C, 1 L FID 250C 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 1-Propanol iso-Butanol 3-Methyl-3-pentanol 1-Butanol 4-Methyl-2-pentanol 1-Pentanol 2-Ethyl-1-Butanol 1-Hexanol Cyclohexanol 1-Heptanol

Column:
P/N. Carrier: Oven:

HP-FFAP
30 m x 0.53 mm id, 1.0 m 19095F-123 Helium, 10 mL/min 60C for 1 min 60-100C at 5C/min 100-210C at 10C/min Split, 10:1 TCD

Injection: Detector:

Injection: Detector:

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11.

Ethylene oxide Ethyl formate Ethyl alcohol Water 2-Ethoxyethanol 2-Ethoxyethyl acetate Hydroxy acetate Acetic acid Formic acid Ethylene glycol/monoformate Ethylene glycol/monoacetate

10

8 9 5 7

10
3 1 2

5 2 1
0

11 7

4 3
5 10 Time (min)

9
15 20

0 C817

6 Time (min)

10

12

GC and GC/MS

C845

Organic Acids
Column:
P/N: Carrier: Oven:

Free Organic Acids/C4-C5 Isomers


Column:
P/N: Oven:

DB-WAXetr
30 m x 0.53 mm id, 1.0 m 125-7332 Helium at 37 cm/sec, measured at 40C 125C for 5 min 125-180C at 15C/min 180C for 12 min Split, 250C FID, 250C

HP-INNOWax
30 m x 0.25 mm id, 0.25 m 19091N-133 110C for 1 min 110-133 at 2C/min 133-160C at 3C/min Helium 42 cm/sec, 24 psi (120C) 1.8 mL/min constant flow Split (40:1), 1 L, 250C FID 300C 1. 2. 3. 4. 5. 6. 7. 8. 9. 1 2 4 3 5 6 8 9 Isobutyric acid Butyric acid Valerolactone 2-Methyl butyric acid Valeric acid 4-Pentenoic acid trans-2-Methyl-2-butenoic acid trans-3-Pentenoic acid trans-2-Pentenoic acid

Carrier: Injection: Detector:

Injector: Detector: 1. 2. 3. 4. 5. 6. 7. 8. 9.

Acetic acid Propionic acid Isobutyric acid Butyric acid Isovaleric acid Valeric acid (pentanoic acid) Isocaproic acid Caproic acid (hexanoic acid) Heptanoic acid

0 C568

C814

10 Time (min)

15

20

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395

GC and GC/MS Applications


Industrial Chemicals
Volatile Amines
Column:
P/N: Oven: Injector: Detector:

Primary Amines
Column:
P/N: Carrier: Oven: Sample: Detector:

DB-1
30 m x 0.53 mm id, 5.0 m 125-1035 30C isothermal Split 1:10, headspace injections FID Nitrogen makeup gas at 30 mL/min

HP-35
15 m x 0.25 mm id, 0.25 m 19091G-131 Helium, 1.5 mL/min constant flow 45C for 2 min 45-220C at 25C/min 1 L split (ratio 30:1), 0.1 to 0.35% aliphatic amines in methanol FID 310C

1. Methylamine 2. Dimethylamine 3. Trimethylamine

1. 2. 3. 4. 5. 6. 7. 8. 9.

n-Butylamine n-Amylamine n-Hexylamine n-Cyclohexylamine n-Heptylamine n-Octylamine n-Decylamine n-Dodecanylamine n-Tetradecanylamine

1 3

GC and GC/MS

C101

C822

6 Time (min)

10

Primary Amines
Column:
P/N: Carrier: Oven: Injector: Detector:

Polyethyleneamines
Column:
P/N: Carrier: Oven: Injector: Detector:

CAM
30 m x 0.25 mm id, 0.25 m 112-2132 Hydrogen at 40 cm/sec 110C isothermal Split FID Nitrogen makeup gas at 30 mL/min

DB-5ms
30 m x 0.25 mm id, 0.5 m 122-5536 Helium at 30 cm/sec, measured at 100C 100C for 1 min 100-320C at 20C/min Split 1:50, 250C 1 L of 100 ng/L standard in methanol FID, 300C Nitrogen makeup gas at 30 mL/min 1. Ethylenediamine 2. Diethylenetriamine 3. Triethylenetetramine 3.* Branched and piperazine analogs of peak #3 4. Tetraethylenepentamine 4.* Branched and piperazine analogs of peak #4

1. 2. 3. 4. 5.

n-Octylamine n-Nonylamine n-Decylamine Benylamine Dicyclohexylamine

C100

C398

396

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GC and GC/MS Applications


Industrial Chemicals
Amines and Nitriles
Column:
P/N: Carrier: Oven: Injector: Detector:

DB-5ms
30 m x 0.25 mm id, 0.5 m 122-5536 Helium at 22 cm/sec, measured at 40C 40C for 1 min 40-260C at 10C/min Split 1:50, 250C 1 L of 100 ng/L standard in methanol FID, 300C Nitrogen makeup gas at 30 mL/min 27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38. 4-Chloroaniline N,N-Diethylaniline n-Decylamine 4-Bromoaniline 3,4-Diaminotoluene 2,6-Diethylaniline 2-Nitroaniline Dicyclohexylamine 3,4-Dichloroaniline 3-Nitroaniline 4-Nitroaniline Diphenylaniline

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26.

Diethylamine Propionitrile Diisopropylamine Triethylamine Pyridine Pyrimidine Pyrazole Acrylamide Pyridazine Aniline 3-Bromopyridine Benzonitrile 3-Cyanopyridine Benzylamine n-Octylamine 1-Methyl-2-pyrrolidine N,N-Dimethylbenzylamine Phenylethylamine N-Benzylmethylamine 2-Cyanopyridine 2-Chloroaniline n-Nonylamine 2,4-Dimethylaniline 4-Chlorobenzonitrile 2,6-Dimethylaniline 3-Chloroaniline

GC and GC/MS

C399

Amines in Water
Column:
P/N: Carrier: Oven: Injector: Detector:

Aldehydes and Acids


Column:
P/N: Carrier: Oven:

CAM
30 m x 0.25 mm id, 0.25 m 112-2132 Hydrogen at 38 cm/sec 120-220C at 10C/min Split FID Nitrogen makeup gas at 30 mL/min 1. 2. 3. 4. 5. 6. 7. Ethylenediamine Piperazine Diethylenetriamine N-(2-Aminoethyl) piperazine Aminoethylethanolamine Triethylenetetramine (4 isomers) Tetraethylenepentamine (4 isomers)

HP-INNOWax
30 m x 0.32 mm id, 0.5 m 19091N-213 Helium, 40 cm/sec, 11.7 psi (60C) 60C for 1 min 60-250C at 10C/min 2.5 mL/min constant flow Split (40:1), 0.5 L, 250C FID 275C

Injection: Detector:

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17.

Butanal 2-Methyl butanal Pentanal Hexanal Heptanal Octanal Acetic acid Decanal Propanoic acid iso-Butyric acid Butyric acid iso-Valeric acid Valeric acid Hexanoic acid Heptanoic acid Octanoic acid Decanoic acid

9 10 11 12 13 16 2 7 1 14 3 5 4 6 8 15 17

C099

C820

10 Time (min)

15

20

www.agilent.com/chem

397

GC and GC/MS Applications


Industrial Chemicals
Aldehydes and Ketones
Column:
P/N: Carrier: Oven: Injector: Detector:

DB-1
30 m x 0.32 mm id, 3.0 m 123-1034 Helium at 32 cm/sec, measured at 40C 40C for 5 min 40-210C at 10C/min Split 1:100, 250C FID, 300C Nitrogen makeup gas at 30 mL/min

GC and GC/MS

Compound list for both chromatograms 1. Acetaldehyde 2. Acrolein 3. Acetone 4. Propionaldehyde 5. Isobutyraldehyde 6. Methacrolein 7. Butyraldehyde 8. 2-Butanone (MEK) 9. Crotonaldehyde 10. 3-Methyl-2-butanone 11. 2-Pentanone 12. 3-Pentanone 13. Valeraldehyde (pentanal) 14. 4-Methyl-2-pentanone (MIBK) 15. 2-Methyl-3-pentanone 16. 3-Hexanone 17. Cyclopentanone 18. 2-Hexanone 19. Hexanal 20. Furfural 21. 4-Heptanone 22. 3-Heptanone 23. 2-Heptanone 24. Cyclohexanone 25. Heptanal 26. Benzaldehyde 27. Octyl aldehyde 28. o-Tolualdehyde 29. m-Tolualdehyde 30. p-Tolualdehyde 31. Nonyl aldehyde

C396

DB-1 provides the best overall resolution; however, DB-WAX provides better resolution of o- and m-tolualdehyde.

Column:
P/N: Carrier: Oven: Injector: Detector:

DB-WAX
30 m x 0.32 mm id, 0.5 m 123-7033 Helium at 32 cm/sec, measured at 40C 40C for 5 min 40-210C at 10C/min Split 1:100, 250C FID, 300C Nitrogen makeup gas at 30 mL/min

C397

398

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GC and GC/MS Applications


Industrial Chemicals
Formaldehyde Underivatized
Column:
P/N: Carrier: Oven: Injector: Detector:

DNPH Derivative
Column:
P/N: Carrier: Oven: Injector: Detector:

PFBHA Derivative
Column:
P/N: Carrier: Oven: Injector: Detector:

DB-WAX
30 m x 0.32 mm id, 0.5 m 123-7033 Helium at 36 cm/sec, measured at 35C 35C isothermal Split 1:100, 200C FID, 300C Nitrogen makeup gas at 30 mL/min

DB-1
15 m x 0.32 mm id, 0.25 m 123-1012 Helium at 35 cm/sec, measured at 150C 150-250C at 20C/min Split 1:100, 300C ECD, 375C Nitrogen makeup gas at 35 mL/min

DB-1
15 m x 0.32 mm id, 0.25 m 123-1012 Helium at 40 cm/sec, measured at 60C 60-100C at 10C/min Split 1:100, 250C FID, 375C Nitrogen makeup gas at 35 mL/min

1. Formaldehyde 2. Methanol *Formaldehyde by-products

1. Formaldehyde-DNPH

1. Formaldehyde-PFBHA

GC and GC/MS

C393

C394

C395

Aromatics
Column:
P/N: Carrier: Oven: Injector: Detector:

DB-1
Prosteel version 30 m x 0.53 mm id, 3.0 m 125-1034 Helium at 30 cm/sec, measured at 40C 40C for 5 min 40-260C at 10C/min Split 1:10, 250C FID, 300C Nitrogen makeup gas at 30 mL/min

1. 2. 3. 4. 5. 6. 7. 8. 9.

Benzene Fluorobenzene Toluene Chlorobenzene Ethylbenzene m-Xylene p-Xylene Styrene o-Xylene

10. 11. 12. 13. 14. 15. 16.

Isopropylbenzene (cumene) Bromobenzene Propylbenzene 2-Chlorotoluene 3-Chlorotoluene 4-Chlorotoluene 1,3,5-Trimethylbenzene (mesitylene)

17. -Methylstyrene 18. tert-Butylbenzene 19. 1,2,4-Trimethylbenzene (pseudocumene) 20. 4-Methylstyrene 21. 1,3-Dichlorobenzene 22. 1,4-Dichlorobenzene 23. Isobutylbenzene 24. sec-Butylbenzene 25. 1,2,3-Trimethylbenzene (hemimellitene) 26. 1,2-Dichlorobenzene 27. Iodobenzene 28. Styrene oxide 29. Butylbenzene 30. 4-Chlorostyrene 31. Nitrobenzene 32. 4-tert-Butyltoluene 33. 1,3,5-Trichlorobenzene 34. 2-Nitrotoluene 35. 1,3-Diisopropylbenzene 36. 1,4-Diisopropylbenzene 37. 1,2,4-Trichlorobenzene 38. 3-Nitrotoluene 39. 4-Nitrotoluene 40. 1,2,3-Trichlorobenzene 41. 1-Chloro-4-nitrobenzene 42. 1,2,4,5-Tetrachlorobenzene 43. Pentachlorobenzene

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399

GC and GC/MS Applications


Industrial Chemicals
Aromatics
Column:
P/N: Carrier: Oven: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. Benzene Fluorobenzene Toluene Chlorobenzene Ethylbenzene m-Xylene p-Xylene Styrene o-Xylene Isopropylbenzene (cumene) 11. Bromobenzene 12. Propylbenzene 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 2-Chlorotoluene 3-Chlorotoluene 4-Chlorotoluene 1,3,5-Trimethylbenzene (mesitylene) -Methylstyrene tert-Butylbenzene 1,2,4-Trimethylbenzene (pseudocumene) 4-Methylstyrene 1,3-Dichlorobenzene 1,4-Dichlorobenzene Isobutylbenzene 24. sec-Butylbenzene 25. 1,2,3-Trimethylbenzene (hemimellitene) 26. 1,2-Dichlorobenzene 27. Iodobenzene 28. Styrene oxide (peak not shown) 29. Butylbenzene 30. 4-Chlorostyrene 31. Nitrobenzene 32. 4-tert-Butyltoluene 33. 1,3,5-Trichlorobenzene 34. 2-Nitrotoluene 35. 1,3-Diisopropylbenzene 36. 1,4-Diisopropylbenzene 37. 1,2,4-Trichlorobenzene 38. 3-Nitrotoluene 39. 4-Nitrotoluene 40. 1,2,3-Trichlorobenzene 41. 1-Chloro-4-nitrobenzene 42. 1,2,4,5-Tetrachlorobenzene 43. Pentachlorobenzene

DB-WAX
-Prosteel version 30 m x 0.53 mm id, 1.0 m 125-7032 Helium at 30 cm/sec, measured at 40C 40C for 5 min 40-230C at 10C/min 230C for 7 min Split 1:10, 250C FID, 300C Nitrogen makeup gas at 30 mL/min

Injector: Detector:

See below for enlarged view

GC and GC/MS
C553

400

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GC and GC/MS Applications


Industrial Chemicals
Impurities in Styrene
Column:
P/N: Carrier: Oven: Injector: Detector:

DB-WAXetr
60 m x 0.32 mm id, 0.5 m 123-7363 Helium at 29.4 cm/sec, measured at 70C 80C isothermal Split 1:150, 230C FID, 240C
11 3 9

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11.

Benzene Toluene Ethylbenzene p-Xylene m-Xylene Isopropylbenzene o-Xylene n-Propylbenzene Styrene -Methylstyrene Heptane (IS)

ASTM D5135
10 6

8 1 4 2 5 7

10 Time (min)

20

30

C588

GC and GC/MS

Impurities in Ethylbenzene
Column:
P/N: Carrier: Oven:

Impurities in Mixed Xylenes


1. 2. 3. 4. 5. 6. 7. 8. 9. 10. Hydrocarbon Benzene Toluene Ethylbenzene p-Xylene m-Xylene Cumene o-Xylene Propylbenzene Styrene

HP-INNOWax
60 m x 0.32 mm id, 0.5 m 19091N-216 Helium, 32 cm/sec, 19.9 psi (60C) 2.5 mL/min constant flow 60C for 1 min 60-92C at 4C/min 92C for 4.5 min 92-220C at 20C/min Split (100:1), 0.5 L, inlet 220C ASTM Method D5060 Neat, 99%+ FID 270C

Column:
P/N: Carrier: Oven:

DB-WAXetr
60 m x 0.32 mm id, 0.25 m 123-7362 Helium at 20 cm/sec, measured at 145C 60C for 10 min 60-150C at 5/min 150C for 10 min Split 1:150, 230C FID, 240C

Injection: Sample: Detector:

Injector: Detector:

ASTM D2360
1. 2. 3. 4. 5. 6. 7. Benzene Toluene Ethylbenzene p-Xylene m-Xylene o-Xylene n-Butylbenzene (IS)

4
4

6
1 6 5 7 3 1 2 1 8 9 10 10 Time (min) 5 C848 10 Time (min) 15 C472 11

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401

GC and GC/MS Applications


Industrial Chemicals
Pyrolysates of Polystyrene
Column:
P/N: Carrier: Oven: Injection: Sample: Detector:

Esters
Column:
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. Propylene Propane 1-Butene Butene Pentane Toluene Styrene C2H5-C(Ph) = CH2 C4H9-CH2CH2-Ph C4H9-C(Ph) = CH2 C4H9-CH=C(Ph)CH3 Styrene dimer Styrene trimer P/N: Carrier: Oven: Injector: Detector:

HP-Ultra 1
50 m x 0.20 mm id, 0.33 m 19091A-105 Helium, 30 psi, 12 mL/min 0-280 at 5C/min Split 30:1, inlet 280C, Pyrolyzer 600C 100 mg pyrolyzed FID 300C

DB-1
-Prosteel version 30 m x 0.53 mm id, 3.0 m 125-1034 1. Helium at 30 cm/sec, 2. measured at 40C 3. 40C for 5 min 4. 40-260C at 10/min 5. Split 1:10, 250C 6. FID, 300C 7. Nitrogen makeup gas 8. at 30 mL/min 9. 10. 11. 12. 13. Methyl formate Ethyl formate Methyl acetate Vinyl acetate Ethyl acetate Propyl formate Methyl propionate Isopropyl acetate Ethyl acrylate tert-Butyl acetate Ethyl propionate Propyl acetate sec-Butyl acetate 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. Isobutyl acetate Propyl propionate Butyl acetate Isoamyl acetate Amyl acetate 2-Ethoxyethyl acetate 2-Methylbutyl acetate Methyl benzoate Benzyl acetate Ethyl benzoate Propyl benzoate

12

13 10 2 1 4 9 5 10 C835 20 8 11 30 Time (min) 40 50 60 C554

GC and GC/MS

3 0

Esters
Column:
P/N: Carrier: Oven:

DB-624
-Prosteel version 30 m x 0.53 mm id, 3.0 m 125-1334 Helium at 30 cm/sec, measured at 40C 40C for 5 min 40-260C at 10/min 260C for 3 min Split 1:10, 250C FID, 300C Nitrogen makeup gas at 30 mL/min

Injector: Detector:

9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24.

Ethyl acrylate tert-Butyl acetate Ethyl propionate Propyl acetate sec-Butyl acetate Isobutyl acetate Propyl propionate Butyl acetate Isoamyl acetate Amyl acetate 2-Ethoxyethyl acetate 2-Methylbutyl acetate Methyl benzoate Benzyl acetate Ethyl benzoate Propyl benzoate

Esters
Column: HP-INNOWax
P/N: Carrier: Oven: Injection: Detector: 30 m x 0.53 mm id, 1.0 m 19095N-123 Helium 29 cm/sec, 3.0 psi (45C) 4 mL/min constant flow 45C for 1 min 45-200C at 5C/min Split (25:1), 1 L, inlet 250C FID 250C

1. 2. 3. 4. 5. 6. 7. 8.

Methyl formate Ethyl formate Methyl acetate Vinyl acetate Ethyl acetate Propyl formate Methyl propionate Isopropyl acetate 1

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19.

Ethyl propionate Propyl acetate Ethyl butyrate Propyl propionate Propyl butyrate Ethyl valerate Butyl propionate Propyl valerate Ethyl caproate Butyl valerate Propyl caproate Methyl decanoate Butyl caproate Methyl dodecanoate Butyl heptanoate Methyl tetradecanoate Methyl hexadecanoate Methyl octadecanoate Methyl eicosenoate

6 2 3 4 5 8

9 15 11 13

10 12 7 14 16 17 18 19

0 C558 C824

10

15 Time (min)

20

25

402

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GC and GC/MS Applications


Industrial Chemicals
Ethers
Column:
P/N: Carrier: Oven: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. Furan Ethyl vinyl ether Ethyl ether 1,3-Dioxolane Methyl-tert-butyl ether (MTBE) Allyl ethyl ether Isopropyl ether Tetrahydrofuran (THF) tert-Butyl ethyl ether Butyl methyl ether Glyme (propylene glycol dimethyl ether) tert-Amyl methyl ether 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. Diglyme (diethylene glycol dimethyl ether) Propyl ether Allyl ether 1,4-Dioxane Butyl ethyl ether Epichlorohydrin Tetrahydropyran Acetal (acetaldehyde diethyl acetal) Butyl ether Pentyl ether Triglyme (triethylene glycol dimethyl ether) Benzyl ether

DB-624
-Prosteel version 30 m x 0.53 mm id, 3.0 m 125-1334 Helium at 30 cm/sec, measured at 40C 40C for 5 min 40-260C at 10/min 260C for 3 min Split 1:10, 250C FID, 300C Nitrogen makeup gas at 30 mL/min

Injector: Detector:

GC and GC/MS

C558

Glycols
Column:
P/N: Carrier: Oven: Injector: Detector:

DB-WAX
30 m x 0.45 mm id, 0.85 m 124-7032 Helium at 35 cm/sec, measured at 50C 50C for 2 min 50-220C at 10/min Megabore Direct, 1 L, 250C FID, 280C Nitrogen makeup gas at 30 mL/min

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19.

Ethylene glycol monomethyl ether Ethylene glycol monoethyl ether 1,3-Propanediol 1,2-Propanediol (propylene glycol) 2,3-Butanediol 1,3-Butanediol Ethylene glycol monobutyl ether Diethylene glycol monomethyl ether 1,4-Butanediol Diethylene glycol monoethyl ether Dipropylene glycol 1,5-Pentanediol 1,6-Hexanediol 1,7-Heptanediol Diethylene glycol monobutyl ether 1,8-Octanediol 1,9-Nonanediol Ethylene glycol 1,10-Decanediol

Glycols Column:
P/N: Carrier: Oven:

DB-624
-Prosteel version 30 m x 0.53 mm id, 3.0 m 125-1334 Helium at 30 cm/sec, measured at 40C 40C for 5 min 40-260C at 10/min 260C for 3 min Split 1:10, 250C FID, 300C Nitrogen makeup gas at 30 mL/min

Injector: Detector:

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22.

Ethylene glycol monomethyl ether Glyme Ethylene glycol Diglyme Ethylene glycol monoethyl ether 1,3-Propanediol 1,2-Propanediol (propylene glycol) 2,3-Butanediol 1,3-Butanediol Ethylene glycol monobutyl ether 1,4-Butanediol Diethylene glycol monomethyl ether Diethylene glycol Diethylene glycol monoethyl ether 1,5-Pentanediol 1,6-Hexanediol Diethylene glycol monobutyl ether Triglyme 1,7-Heptanediol 1,8-Octanediol 1,9-Nonanediol 1,10-Decanediol

C405

C561

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403

GC and GC/MS Applications


Industrial Chemicals
Glycols
Column:
P/N: Carrier: Oven: Injector: Detector:

Triethylene Glycol and Impurities


DB-1
-Prosteel version 30 m x 0.45 mm id, 1.27 m 124-1032 Helium at 35 cm/sec, measured at 50C 50C for 2 min 50-260C at 10/min Megabore Direct, 1 L, 250C FID, 280C Nitrogen makeup gas at 30 mL/min 1. 2. 3. 4. 5. 6. 7. 8. 9. Ethylene glycol monomethyl ether Ethylene glycol monoethyl ether 1,3-Propanediol 1,2-Propanediol 2,3-Butanediol 1,3-Butanediol Ethylene glycol monobutyl ether Diethylene glycol monomethyl ether 1,4-Butanediol 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. Diethylene glycol monoethyl ether Dipropylene glycol 1,5-Pentanediol 1,6-Hexanediol 1,7-Heptanediol Diethylene glycol monobutyl ether 1,8-Octanediol 1,9-Nonanediol Ethylene glycol 1,10-Decanediol

Column:
P/N: Carrier: Oven: Injector: Detector:

DB-1
30 m x 0.45 mm id, 1.27 m 124-1032 Helium at 35 cm/sec, measured at 50C 170C isothermal Split 1:50, 0.5 L, 250C FID, 280C Nitrogen makeup gas at 30 mL/min

1. Ethylene glycol 2. Diethylene glycol 3. Triethylene glycol

GC and GC/MS

C404

C406

Ethylene Glycol Mixture


Column:
P/N: Carrier: Oven: Injection: Detector:

Glycols/Diols
Column:
P/N: Carrier: Pressure program: Oven: 1. 2. 3. 4. 5. Diethylene glycol Triethylene glycol Polyethylene glycol 200 Polyethylene glycol 400 Polyethylene glycol 600 Injection: Inlet program: Detector:

Ultra-1
12 m x 0.2 mm id, 0.33 m 19091A-101 Helium, 25 cm/sec 100C for 0.5 min 100-200C at 20C/min Split (100:1), 250C, 1 L FID

HP-1
30 m x 0.53 mm id, 0.88 m 19095Z-023 Helium 10 psi to 25 psi (4.25 min) at 1 psi/min 50C for 3 min 50-180C at 8C/min On-column, 1 L 50C (0 min) to 180C (8.45 min) at 12C/min FID 250C 2 1 1. 2. 3. 4. 5. 6. Ethylene glycol 1,3-Butanediol Ethylene glycol phenyl ether 1,7-Hepatanediol 1,9-Nonanediol 1,10-Decanediol

1 3

4
5 2 4 5 6

7 min 20 min

C818

C819

404

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GC and GC/MS Applications


Industrial Chemicals
Halogenated Hydrocarbons
Column:
P/N: Carrier: Oven: Injector: Detector: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. Pentane Iodomethane 1,1-Dichloroethene 1,1,2-Trichlorotrifluoroethane (Freon-113) 3-Chloropropene (allyl chloride) Methylene chloride 1,1-Dichloroethane Chloroform 1,1,1-Trichloroethane 1-Chlorobutane Carbon tetrachloride 1,2-Dichloroethane 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 1,2-Dichloropropane cis-1,2-Dichloropropene trans-1,2-Dichloropropene 1,1,2-Trichloroethane 1,1,1,2-Tetrachloroethane 1,2-Dibromoethane (EDB) 1-Chlorohexane trans-1,4-Dichloro-2-butene Iodoform Hexachlorobutadiene 1,2,3-Trichloropropane 1,1,2,2-Tetrachloroethane Pentachloroethane 1,2-Dibromo-3-chloropropane (DBCP) Hexachloroethane Hexachlorocyclopentadiene

DB-624
30 m x 0.32 mm id, 1.8 m 123-1334 Helium at 35 cm/sec 35C for 5 min 35-245C at 10/min Split 1:50, 250C FID, 300C Nitrogen makeup gas at 30 mL/min

GC and GC/MS

C215

Halogenated Hydrocarbons
Column:
P/N: Carrier: Oven:

DB-1
30 m x 0.32 mm id, 3.0 m 123-1034 Helium at 35 cm/sec, measured at 35C 35C for 5 min 35-245C at 10/min 245C for 2 min Split 1:100 in pentane, 250C FID, 300C Nitrogen makeup gas at 30 mL/min

Injector: Detector:

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38.

1,1,2-Trichlorotrifluoroethane (Freon-113) 1,1-Dichloroethene Bromoethane (ethyl bromide) Iodomethane 3-Chloropropene (allyl chloride) 1-Chlorobutane 2,2-Dichloropropane trans-1,2-Dichloroethene 1,1,1-Trichloroethane Carbon tetrachloride Methylene chloride Trichloroethene Chloroform Tetrachloroethene 1,2-Dichloropropane 1-Chlorohexane Bromochloromethane 1,1-Dichloroethane 1,2-Dichloroethane Iodoform cis-1,3-Dichloropropene Dibromomethane Bromodichloromethane 1,3-Dichloropropane 1,1-Dichloropropane trans-1,3-Dichloropropene 1,1,2-Trichloroethane 1,2-Dibromoethane (EDB) 1,1,1,2-Tetrachloroethane Pentachloroethane Hexachloroethane Bromoform trans-1,4-Dichloro-2-butene 1,2,3-Trichloropropane Hexachlorobutadiene 1,1,2,2-Tetrachloroethane 1,2-Dibromo-3-chloropropane (DBCP) Hexachlorocyclopentadiene

C113

www.agilent.com/chem

405

GC and GC/MS Applications


Industrial Chemicals
Chlorinated Isooctane
Column:
P/N: Carrier: Oven: Injection: Detector:

HP-INNOWax
60 m x 0.25 mm id, 0.25 m 19091N-136 Helium, 33 cm/sec, 35.7 psi (80C) 2 mL/min 80C isothermal Split (150:1), 0.5 L, 250C 1. 1-Chloro isooctane FID 300C 2. 4-Chloromethyl 2,2'-dimethyl pentane 3. 3-Chloro isooctane 4. 4-Chloro isooctane

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4 2 3

Monochloro isomers

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6 7

GC and GC/MS

3 C829

4 Time (min)

Solvents
Column:
P/N: Carrier: Oven: Injector: Detector:

DB-WAXetr
30 m x 0.53 mm id, 1.0 m 125-7332 Helium at 30 cm/sec, measured at 40C 40C for 5 min 40-140C at 5/min Split, 250C FID, 250C

1. 2. 3. 4. 5. 6. 7. 8.

3-Methylpentane Hexane Isooctane Methyl-tert-butyl ether (MTBE) Heptane Cyclohexane Octane Nonane

9. 10. 11. 12. 13. 14. 15. 16. 17. 18.

Methanol Ethanol Benzene Decane Toluene Undecane Ethylbenzene p-Xylene m-Xylene Cumene

19. 20 21. 22. 23. 24. 25. 26. 27. 28. 29. 30.

Dodecane o-Xylene Propylbenzene Chlorobenzene Mesitylene Styrene 1,2,4-Trimethylbenzene Naphthalene 4-Chlorotoluene 1,3-Dichlorobenzene 1,4-Dichlorobenzene 1,2-Dichlorobenzene

C569

406

www.agilent.com/chem

GC and GC/MS Applications


Industrial Chemicals
Solvents
Column:
P/N: Carrier: Oven: Injector: Detector:

DB-WAXetr
50 m x 0.32 mm id, 1.0 m 123-7354 Helium at 35.2 cm/sec, measured at 50C 50C for 5 min 50-170C at 10/min Split 1:100, 250C FID, 280C Nitrogen makeup gas at 30 mL/min

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19.

Hexane Isooctane Acetone Ethyl formate Tetrahydrofuran Trichloroethane Ethyl acetate Isopropyl acetate Methyl ethyl ketone Methylene chloride Isopropyl alcohol Benzene 2-Pentanone Methyl isobutyl ketone Isobutyl acetate Chloroform sec-Butyl alcohol Toluene n-Propanol

GC and GC/MS

C420

Aromatic Solvents
Column:
P/N: Carrier: Oven: Injector:

DB-200
30 m x 0.25 mm id, 0.25 m 122-2032 Helium at 31 cm/sec 50C for 5 min 50-160C at 10/min Split 1:100, 250C 0.5 L of 0.5 g/L standard in hexane FID, 300C Nitrogen makeup gas at 30 mL/min

Detector:

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12.

Benzene Toluene Ethylbenzene Chlorobenzene p-Xylene m-Xylene o-Xylene Styrene Isopropylbenzene n-Propylbenzene 2-Chlorotoluene 3-Chlorotoluene

13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25.

4-Chlorotoluene tert-Butylbenzene sec-Butylbenzene Isobutylbenzene 1,3-Dichlorobenzene 1,4-Dichlorobenzene n-Butylbenzene 1,2-Dichlorobenzene 1,3-Diisopropylbenzene 1,4-Diisopropylbenzene 2-Nitrotoluene 3-Nitrotoluene 4-Nitrotoluene

C505

www.agilent.com/chem

407

GC and GC/MS Applications


Industrial Chemicals
Solvents
Column:
P/N: Carrier: Oven: Injector: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 21 23 24 1 9 14 20 19 22 Methanol Ethanol Ethyl ether Isopropanol n-Hexane Methylene chloride tert-Butanol n-Propanol Chloroform Cyclohexane sec-Butanol n-Heptane Acetone Acetonitrile Benzene Tetrahydrofuran (THF) Trichloroethylene n-Butanol Ethyl acetate Methyl ethyl ketone (MEK) Toluene 1,4-Dioxane Pyridine Dimethylformamide (DMF)

DB-200
30 m x 0.25 mm id, 0.5 m 122-2033 Helium at 31 cm/sec 45C for 7 min 45-145C at 20/min Split 1:00, 250C 0.5 L of 0.5-1.0 g/L standard in water FID, 300C Nitrogen makeup gas at 30 mL/min

11,12,13

Detector:

15 6 2,3 5 4 16 18 7 10 8 17

GC and GC/MS

0
C504

6 Time (min)

1 2

Solvents
Column:
P/N: Carrier: Oven: Injection: Detector:

HP-1
50 m x 0.2 mm id, 0.52 m 19091Z-205 Helium, 30 psi 70-200C at 5C/min 200C for 2 min Split, 1 L TCD

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14.

Isopropanol Methyl ethyl ketone Ethyl acetate n-Butyl alcohol Ethyl cellosolve Methyl isobutyl ketone Toluene n-Butyl acetate Diacetone alcohol p-Xylene Cellosolve acetate o-Xylene Butyl cellosolve Butyl cellosolve acetate

Common Industrial Solvents


Column: HP-1
P/N: Carrier: Oven: Injection: Detector: 25 m x 0.32 mm id, 1.0 m 19091Z-212 Helium, 35 kPa 30-140C at 10C/min Split (200:1), 1 L IRD 200C 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. Methanol Methyl formate Ethanol Acetone Isopropanol Dichloromethane n-Propanol Methyl ethyl ketone sec-Butanol Ethyl acetate Isobutanol Isopropyl acetate Nitropropane 1,4-Dioxane Toluene Mesityl oxide Diacetone-alcohol m-Xylene Cyclohexanone

12 2 3 4 9 7 5 6 10 8 13 11 12 13 14 1 8 2 3 1 45 6 7 9 11 10

19 17

14

16 18 15

25 min C847 C839

12 min

408

www.agilent.com/chem

GC and GC/MS Applications


Industrial Chemicals
Rapid Analysis of Water in Organic Solvents
Column:
P/N: Carrier: Oven: Post temp: Inlet: Injector: Injection volume: Detector:

Nitrogen Based Solvents


Column:
P/N: Carrier: Oven: Injector: Detector:

HP-INNOWax
15 m x .53 mm id, 1.0 m 19095N-121 15 mL/min helium constant flow mode 100C for 1.5 min 175C (2.5 min) Purge packed 240C 0.5 L, 87 ppm Water in Aniline Thermal conductivity (TCD), 240C

DB-1
-Prosteel Version 30 m x 0.53 mm id, 3.0 m 125-1034 Helium at 30 cm/sec, measured at 40C 40C for 5 min 40-260C at 10/min Split 1:10, 250C FID, 300C Nitrogen makeup gas at 30 mL/min

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13.

Acetonitrile Acrolein Acrylonitrile Propionitrile Methacrolein Methacrylonitrile Triethylamine Ethyl acrylate Pyridine DMF (dimethylformamide) DMSO (dimethyl sulfoxide) Benzonitrile 1-Methyl-2-pyrrolidinone

12

CS 2 1
87 ppm Water in Aniline

13

7 8 10 11

0 C846

0.2

0.4

0.6

0.8 Time (min)

1.0

1.2

0
C565

8 10 Time (min)

12

14

16

18

GC and GC/MS

Nitrogen Based Solvents


Column:
P/N: Carrier: Oven:

DB-624
-Prosteel version 30 m x 0.53 mm id, 3.0 m 125-1334 Helium at 30 cm/sec, measured at 40C 40C for 5 min 40-260C at 10/min 260C for 3 min Split 1:10, 250C FID, 300C Nitrogen makeup gas at 30 mL/min

Injector: Detector:

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13.

Acetonitrile Acrolein Acrylonitrile Propionitrile Methacrolein Methacrylonitrile Triethylamine Ethyl acrylate Pyridine DMF (dimethylformamide) DMSO (dimethyl sulfoxide) Benzonitrile 1-Methyl-2-pyrrolidinone

C566

www.agilent.com/chem

409

GC and GC/MS Applications


Industrial Chemicals
Acrylate Impurities
Column:
P/N: Carrier: Oven: Injector: Detector: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. Methanol Ethanol Methyl acetate Ethyl acetate Methyl acrylate Methyl propionate Isobutanol Butanol Ethyl acrylate Ethyl propionate Methyl methacrylate Isopropyl acrylate Isobutyl acetate Butyl acetate Isobutyl acrylate Dibutyl ether Isobutyl propionate Butyl acrylate Butyl propionate

Acrylate Impurities
Column:
P/N: Carrier: Oven: Injector: Detector:

DB-200
30 m x 0.53 mm id, 1.0 m 125-2032 Helium at 34.5 cm/sec measured at 35C 35C for 5 min, 35 - 200C at 10/min Split 1:10, 230C FID, 250C

DB-1701
-Prosteel version 30 m x 0.53 mm id, 1.0 m 125-0732 Helium at 36.8 cm/sec measured at 35C 35C for 5 min, 35-200C at 10/min Split 1:10, 230C FID, 250C

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19.

Methanol Ethanol Methyl acetate Ethyl acetate Methyl acrylate Methyl propionate Isobutanol Butanol Ethyl acrylate Ethyl propionate Methyl methacrylate Isopropyl acrylate Isobutyl acetate Butyl acetate Isobutyl acrylate Dibutyl ether Isobutyl propionate Butyl acrylate Butyl propionate

Methyl acrylate impurities * Ethyl acrylate impurities ] Butyl acrylate impurities

Methyl acrylate impurities Ethyl acrylate impurities ] Butyl acrylate impurities

GC and GC/MS

C694

C693

Acrylates
Column: HP-FFAP
P/N: Carrier: Oven: 10 m x 0.53 mm id, 1.0 m 19095F-121 Hydrogen 35C for 1 min 35-60C at 10C/min 60-160C at 15C/min On-column, 1 L FID

Monomers in Latex Paint by Headspace


Column:
P/N: Carrier: Oven: 1. 2. 3. 4. 5. 6. 7. 8. 9. Methyl methacrylate Ethyl methacrylate sec-Butyl methacrylate Allyl acrylate n-Butyl acrylate Hexyl methacrylate Cyclohexyl methacrylate Hydroxypropyl acrylate Unknown

HP-INNOWax
30 m x 0.53 mm id, 1.0 m 19095N-123 Helium, 45.5 cm/sec (40C) 6 mL/min constant flow 40C for 5 min 40-90C at 15C/min 90-200C at 30C/min Split (10:1), 1 mL headspace, inlet temp 220C 0.200 gm sample with 1 gram sodium sulfate in 5 mL water FID 300C

Injection: Detector:

Injection: Sample: Detector:

1. Acrylonitrile 2. Methyl acrylate 3. Butyl acrylate 4 Styrene

4 3

7 2 6 8

2 1

4 1 9

11 min C815 C834

6 Time (min)

10

12

410

www.agilent.com/chem

GC and GC/MS Applications


Industrial Chemicals
Anilines
Column:
P/N: Carrier: Oven:

DB-35ms
25 m x 0.20 mm id, 0.33 m 128-3822 1 m x 0.32 mm id, retention gap Helium at 35 cm/sec, measured at 50C 50C for 2 min 50-340C at 20/min 340C for 10 min Splitless, 280C, 1 L of 5 ng on-column per component 0.50 min purge activation time FID, 320C Nitrogen makeup gas at 30 mL/min

Injector:

Detector:

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17.

o-Toluidine 4-Chloroaniline 2-Methoxy-5-methylaniline 2,4,5-Trimethylaniline 4-Chloro-2-methylaniline 2,4-Diaminotoluene 2,4-Diaminoanisole 2-Aminonaphthalene 2-Methyl-5-nitroaniline 4,4-Oxydianiline 4,4-Methylenedianiline Benzidine 2-Aminoazotoluene o-Tolidine 4,4-Thiodianiline 3,3-Dimethoxybenzidine 3,3-Dichlorobenzidine

GC and GC/MS

C407

Substituted Anilines
Column:
P/N: Carrier: Oven:

DB-5ms
30 m x 0.25 mm id, 0.5 m 122-5536 Helium at 33.3 cm/sec, measured at 150C 40C for 5 min 40-290C at 12/min 290C for 10 min Splitless, 250C 30 sec purge activation time 1 L of 25 ng/L standard MSD, 325C transfer line

Injector:

Detector:

Compound list for all chromatograms 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. Aniline 2-Chloroaniline 2,6-Dimethylaniline 3-Chloroaniline 4-Chloroaniline 4-Bromoaniline 2-Nitroaniline 3,4-Dichloroaniline 3-Nitroaniline 2,4,5-Trichloroaniline 4-Chloro-2-nitroaniline 4-Nitroaniline 2-Chloro-4-nitroaniline 2,6-Dichloro-4-nitroaniline 2-Chloro-4,6-dinitroaniline 2,6-Dibromo-4-nitroaniline 2,4-Dinitroaniline 2-Bromo-4,6-dinitroaniline (m/z) (93) (127) (121) (127) (127) (171) (138) (161) (65) (195) (172) (138) (172) (176) (217) (266) (183) (261)

C408

www.agilent.com/chem

411

GC and GC/MS Applications


Industrial Chemicals
Phenols
Column: HP 5MS
P/N: Carrier: Oven: Injection: Sample: Purge Time: Detector: 30 m x 0.25 mm id, 0.25 m 19091-5433 Helium, 33 cm/sec, constant flow 35C for 5 min 35-220C at 8C/min 1 L, Splitless, 250C 20 g/mL phenols in methylene chloride 0.75 min FID 300C 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. Phenol 2-Chlorophenol 2-Nitrophenol 2,4-Dimethylphenol 2,4-Dichlorophenol 4-Chloro-3-methylphenol 2,4,6-Trinitrophenol 2,4-Dinitrophenol 4-Nitrophenol 2-Methyl-4,6-dinitrophenol Pentachlorophenol

4 1 5 2 3

6 7 8 9 10 11

0 C832

10 Time (min)

15

20

25

GC and GC/MS

Phenols
Column:
P/N: Carrier: Oven: Injector:

DB-5ms
30 m x 0.25 mm id, 0.5 m 122-5536 Helium at 22 cm/sec, measured at 100C 100C for 1 min 100-270C at 10/min Split 1:50, 250C 1 L of 50 ng/L standard in toluene/p-xylene FID, 300C Nitrogen makeup gas at 30 mL/min

Detector:

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16.

Phenol 2-Chlorophenol o-Cresol m-Cresol p-Cresol 2,6-Xylenol 2,4-Xylenol 2,5-Xylenol 2-Nitrophenol 3,5-Xylenol 2,3-Xylenol 2,4-Dichlorophenol 3,4-Xylenol 2,4,6-Trichlorophenol 2,4-Dinitrophenol 1-Naphthol

C400

412

www.agilent.com/chem

GC and GC/MS Applications


Industrial Chemicals
Phenols
Column:
P/N: Carrier: Oven: Injector: Detector: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 2,6-Xylenol 2-Cresol Phenol 2-Ethylphenol 2,5-Xylenol 4-Cresol 2,4-Xylenol 3-Cresol 2-Isopropylphenol 2,3-Xylenol 3,5-Xylenol 4-Ethylphenol 3,4-Xylenol 2,3,5-Trimethylphenol

DB-WAX
30 m x 0.25 mm id, 0.25 m 122-7032 Hydrogen at 43 cm/sec 165C isothermal Split 1:50, 1 L, 250C FID, 300C Nitrogen makeup gas at 30 mL/min

GC and GC/MS

C117

Halocarbons
Column:
P/N: Carrier: Oven:

GS-GasPro
30 m x 0.32 mm id 113-4332 Helium at 30 cm/sec 130C for 4 min 130-225C at 10/min 225C Hold Split 1:67, 1 L, 250C FID, 250C

Injector: Detector: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22.

CH4 CHCIF2 (Freon 22) CCI2F2 (Freon 12) CICF2 CF2 Cl (Freon 114) CHCI2 F (Freon 21) CCI3F (Freon 11) CF2Br2 (Freon 12B2) CH3I CH2CI2 trans-CICH=CHCI CF3CCl3 (Freon 113) cis-CICH=CHCI CHCI3 ? from CCI4 CCI4 ? from CCI4 CH3CH2I CH2Br2 CHCI2Br C4F9I CHCIBr2 CH3CH2CH2I

C532

www.agilent.com/chem

413

GC and GC/MS Applications


Industrial Chemicals
Ethylene Oxide
(Courtesy of J. Chromatogr. Sci., 28:97 [1990])

Halothane
Column:
P/N: Carrier: Oven: Injector: Detector:

GS-GasPro
15 m x 0.32 mm 113-4312 Helium at 45 cm/sec 240C, isothermal Split 1:100, 0.2 L, 200C FID, 200C

Column:
P/N: Carrier: Oven: Injector: Detector:

DB-WAX
122-7032 Helium at 1 mL/min 60C for 2 min 60-180C at 16/min Split 1:50, 250C FID, 300C Nitrogen makeup gas at 30 mL/min

1. Ethylene oxide 2. 2-Chloroethanol 3. Ethylene glycol (solvent: Dimethylformamide)

1. 2. 3. 4.

Nitrogen Halothane Diethyl ether Acetone

GC and GC/MS

C116

C591

Inorganic Gases
Column:
P/N:

Inorganic Hydride Gases


Column: HP-1
Carrier: Oven: Helium at 53 cm/sec 25C for 3 min 25-200C at 10/min 200C Hold Split 1:50, 200C, 50 L TCD, 250C P/N: Carrier: Oven: Injection: Detector: 50 m x 0.2 mm id, 0.5 m 19091Z-205 Helium, 35 cm/sec A: -70C isothermal B: 30C isothermal Split (25:1), 1 L FPD (535 m filter) 1. Arsine 2. Phosphine 3. Selenide 4. Diborane 5. Tetraborane 6. Pentaborane 7. Dihydropentaborane 0.1% 0.1% 0.1% 0.10 ppm 0.10 ppm 0.10 ppm 0.60 ppm

GS-GasPro
30 m x 0.32 mm id 113-4332

Injector: Detector: 1. 2. 3. 4. 5. 6. 7.

Nitrogen CO2 SF6 COS H2S Ethylene oxide SO2

(A) -70C

(B) 30C

5 6 3 7

C531

C828

414

www.agilent.com/chem

GC and GC/MS Applications


Life Sciences

Life Sciences
Common Drug Screen
Column:
P/N:

DB-5
30 m x 0.25 mm id, 0.25 m 122-5032

Peak 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33. 34.

Drug Nicotine Phenmetrazine Ibuprofen Procaine Allobarbital Aprobarbital Butabarbital Secobarbital Pentobarbital Phenacetin Amobarbital Benzphetamine Acetaminophen Hydroxyphenamate Dimenhydrinate Meprobamate Benactyzine Hexobarbital Doxylamine Caffeine Chlorpheniramine Methapyrilene Thenyldiamine Phenobarbital Bromopheniramine Chlorcyclizine Cocaine Pyrrobutamine Codeine Diazepam Morphine Hydrocodone Oxymorphone Heroin

DB-17 Time 9.87 11.80 12.06 13.48 13.91 14.14 14.56 14.87 15.41 15.72 15.87 16.14 16.34 16.47 16.93 17.12 17.26 17.52 17.69 18.05 18.47 18.72 18.87 19.11 19.71 20.75 21.32 22.79 24.27 25.27 25.36 25.98 28.27 29.32

DB-5 Time 8.57 9.95 10.64 14.82 12.02 12.27 12.76 14.31 13.73 12.94 13.43 14.96 11.12 15.31 13.79 14.44 14.71 15.22 15.87 13.11 16.35 16.68 16.85 16.29 17.39 19.13 18.88 20.89 20.66 21.13 21.12 21.26 22.21 23.14

Column:
P/N: Carrier: Oven:

DB-17
30 m x 0.25 mm id, 0.25 m 122-1732 Hydrogen at 41 cm/sec, measured at 80C 80C for 1 min 80-280C at 10/min 280C for 9 min Split 1:40, 250C FID, 300C

Injector: Detector:

GC and GC/MS

DB-5

DB-17

C131

www.agilent.com/chem

415

GC and GC/MS Applications


Life Sciences
Drug Screen
Column:
P/N: Carrier: Oven:

DB-1ms
30 m x 0.25 mm id, 0.25 m 122-0132 Helium at 40 cm/sec, measured at 50C 50C for 1.0 min 50-125C at 25/min 125-325C at 10/min 325C for 5 min Cold Splitless using Optic II injector 50-250C at 10/sec, 45 sec purge activation time 1 L injection of 50-150 ppm standard FID, 300C

1. 2. 3. 4. 5. 6. 7. 8. 9.

Nicotine Caffeine Glutethimide Lidocaine PCP Phenobarbital Methadone primary metabolite Methaqualone Methadone

10. 11. 12. 13. 14. 15. 16. 17. 18.

Cocaine Desipramine Carbazepine Trimipramine Heroin Fentanyl Ibogaine Triazolam LSD

Injector:

Sample: Detector:

C759

GC and GC/MS

Urine Drug Screen


Column:
P/N: JCarrier: Injector: Oven: Detector:

Amphetamines and Precursors - TMS Derivatives


Column:
P/N: Carrier: Oven: Injector: Detector: 1. 2. 3. 4. 5. 6. 7. 8.

Ultra 2
50 m x 0.32 mm id, 0.52 m 19091B-115 Hydrogen 80 cm/sec Splitless 45C for 1.5 min 45-300C at 6C/min FID

DB-5
20 m x 0.18 mm id, 0.4 m 121-5023 Helium at 39 cm/sec, measured at 100C 100-240C at 10/min Split 1:100, 250C 1 L of 2 g/L each in pyridine FID, 300C Nitrogen makeup gas at 30 mL/min 9. 10. 11. 12. 13. 14. 15. 16. Phenacetin 3,4-Methylenedioxyamphetamine (MDA) 3,4-Methylenedioxymethylamphetamine 4-Methyl-2,5-dimethoxyamphetamine (STP) Phenyl ephedrine 3,4-Methylenedioxyethylamphetamine (MDE; Eve) Caffeine Benzphetamine

12

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13.

Amphetamine Methamphetamine Meperidine Phencyclidine (PCP) Methadone Proposyphene Amitriptyline Cocaine Imipramine Cyheptamide (ISTD) Codeine Diazepam Flurazepam

Phenylacetone Dimethylamphetamine Amphetamine Phentermine Methamphetamine Methyl ephedrine Nicotinamine Ephedrine

8 6 7

10

13 11 12

12 min

C100B

C123

416

www.agilent.com/chem

GC and GC/MS Applications


Life Sciences
Anesthetics
Column:
P/N: Carrier: Oven:

Anticonvulsants
Column:
P/N: Carrier: Oven: Injector: Detector: 1. 2. 3. 4. 5.

DB-5ms EVDX
25 m x 0.20 mm id, 0.33 m 128-8522 Helium at 35 cm/sec, measured at 55C 55C for 1 min 55-130C at 25/min 130-325C at 15/min Splitless, 250C 45 sec purge activation time 1 L of 50-100 ng/L standard in methanol MSD, 280C transfer line full scan at m/z 35-400

DB-1
30 m x 0.53 mm id, 1.5 m 125-1032 Helium at 8 mL/min 160C for 2 min 160-275C at 15/min Megabore Direct, 250C 1 L of 100 ng/L in methanol FID, 300C Nitrogen makeup gas at 30 mL/min 6. 7. 8. 9. 10. Phenobarbital Primidone Carbamazepine Phenytoin 5-Methyl-5-phenylhydantoin

Injector:

Detector:

1. 2. 3. 4. 5. 6. 7. 8. 9.

Salicylamide Benzocaine Lidocaine Procaine Nefopam Mepivacaine Tetracaine Butacaine Dibucaine

Ethosuximide Methsuximide Phensuximide N-Desmethyl methsuximide Phenylethylmalonamide

C216

C141

GC and GC/MS

Antihistamines
Column:
P/N: Carrier: Oven:

DB-5
30 m x 0.32 mm id, 0.25 m 123-5032 Helium at 40 cm/sec, measured at 55C 55C for 1 min 55-175C at 30/min 175-320C at 10/min 320C for 1 min Splitless, 250C 30 sec purge activation time 1 L of 50 ng/L each in methanol FID, 300C Nitrogen makeup gas at 30 mL/min 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. Pheniramine Dimenhydrinate Diphenhydramine Doxylamine Phenyltoloxamine Tripelennamine Methapyrilene Chlorpheniramine Cyclizine Carbinoxamine Diphenylpyraline Bromopheniramine 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. Thonzylamine Chlorcyclizine Pyrilamine Triprolidine Promethazine Antazoline Clemizole Hydroxyzine Meclizine Cinnanzine Buclizine

Injector:

Detector:

C138

www.agilent.com/chem

417

GC and GC/MS Applications


Life Sciences
Antiepileptic Drugs
Column:
P/N: Carrier: Injection: Oven: Detector:

Tricyclic Antipsychotics
Column:
P/N: Carrier: Oven:

Ultra 2
12 m x 0.32 mm id, 0.17 m 19091B-012 Helium, 14 psi Split, 35:1 100-230C at 15C/min NPD 1. 2. 3. 4. 5. 6. 7. 8. Ethotoin PEMA n-Des Phenobarbital Carbepoxide 10/11 Primidone Phenyltoin ISTD

Ultra 2
12 m x 0.2 mm id, 0.33 m 19091B-101 Hydrogen, 106 cm/sec 250C for 3 min 250-290C at 10C/min 290C for 10 min Split, 75:1 FPD 5954-7614 4 3

Injector: Detector: Pub No.:

1 2

2 1

1. 2. 3. 4. 5.

Triflupromazine Promethazine Promazine Chlorpothixene Thioridazine


5

5 3 7

C1012

GC and GC/MS

C1010

Barbiturates
Column:
P/N:

DB-35ms
30 m x 0.25 mm id, 0.25 m 122-3832

Carrier: Oven:

Injector: Detector:

Helium at 31 cm/sec, measured at 50C 50C for 0.5 min 50-150C at 25/min 150-300C at 10/min Splitless, 250C 30 sec purge activation time MSD, 280C transfer line full scan at m/z 40-270

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18.

Barbital Allobarbital Aprobarbital Butabarbital Butethal Butalbital Amobarbital Talbutal Pentobarbital Methohexital Secobarbital Hexobarbital Thiopental Cyclopentylbarbital Mephobarbital Thiamylal Phenobarbital Alphenal

C572

418

www.agilent.com/chem

GC and GC/MS Applications


Life Sciences
Benzodiazepines
Column:
P/N: Carrier: Oven:

DB-35ms
30 m x 0.25 mm id, 0.25 m 122-3832 Helium at 31 cm/sec, measured at 50C 50C for 0.5 min 50-150C at 25/min 150-340C at 10/min 340C for 6 min Splitless, 250C 30 sec purge activation time MSD, 280C transfer line full scan at m/z 40-400

Injector: Detector:

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17.

Medazepam Halazepam Oxazepam Lorazepam Diazepam Demoxepam Desmethyldiazepam Clobazam Temazepam Flunitrazepam Delorazepam Bromazepam Prazepam Flurazepam Clonazepam Alprazolam Triazolam

C574

GC and GC/MS

Fentanyls
Column:
P/N: Carrier: Oven: Injector: Detector:

Tocopherols
DB-1701
30 m x 0.53 mm id, 1.0 m 125-0732 Hydrogen at 15 mL/min 270C isothermal Split 1:5, 0.8 L, 250C FID, 300C Nitrogen makeup gas at 30 mL/min

Column:
P/N: Carrier: Oven:

DB-17ms
30 m x 0.25 mm id, 0.25 m 122-4732 Helium at 40 cm/sec, measured at 150C 300C for 1 min 300-320C at 25/min 320C for 4 min Split 1:25, 310C 1 L of 1-10 ng/L in isooctane MSD, 310C transfer line full scan at m/z 45-550

Injector: 1. 2. 3. 4. 5. Fentanyl Sufentanyl Carfentanyl Lofentanyl Alfentanyl Detector:

1. 2. 3. 4.

-Tocopherol -Tocopherol -Tocopherol -Tocopherol

C129

C474

www.agilent.com/chem

419

GC and GC/MS Applications


Life Sciences
Hallucinogens
Column:
P/N: Carrier: Oven: Helium at 30 cm/sec, measured at 50C 50C for 0.5 min 50-125C at 25/min 125-255C at 10/min 255-320C at 25/min 320C for 16 min Splitless, 250C 30 sec purge activation time 1 L of 10-50 ng/L standard in methanol MSD, 300C transfer line full scan at m/z 40-350 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 4-Methylaminorex Mescaline Dimethyltryptamine Ketimine TCP (Tenocyclidine) PCP Diethyltryptamine Bufotenine Fentanyl Ibogaine LSD Psilocin (peak not shown)

DB-17ms
30 m x 0.25 mm id, 0.25 m 122-4732

Injector:

Detector:

C468

GC and GC/MS

Sedative Hypnotics
Column:
P/N: Carrier: Oven:

Narcotics
Column:
P/N: Carrier: Oven:

DB-5ms EVDX
12 m x 0.20 mm id, 0.33 m 128-8512 Helium at 35 cm/sec, measured at 55C 55C for 1 min 55-130C at 25/min 130-325C at 15/min 325C for 4 min Splitless, 250C 45 sec purge activation time 1 L of 50-100 ng/L standard in methanol MSD, 280C transfer line full scan at m/z 35-400

DB-5ms
30 m x 0.25 mm id, 0.25 m 122-5532 Helium at 31 cm/sec, measured at 50C 50C for 0.5 min 50-150C at 25/min 150-325C at 10/min Splitless, 250C 30 sec purge activation time MSD, 300C transfer line full scan at m/z 40-380 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. Dextromethorphan Codeine Dihydrocodeine Norcodeine Ethylmorphine Morphine Normorphine 6-Acetylcodeine 6-Monoacetylmorphine Heroin

Injector: Detector

Injector:

Detector: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. Ethchlorvynol Ethinamate Pyrithyldione Talbutal Meprobamate Glutethimide Methaqualone Propiomazine Haloperidol Sulpiride Droperidol

2,3

5 9 4 6 7

10

C218

C424

420

www.agilent.com/chem

GC and GC/MS Applications


Life Sciences
Narcotics and Adulterants
Column:
P/N: Carrier: Oven: Injector: Detector:

Over-the-Counter Pain Killers - TMS Derivatives


Column:
P/N: Carrier: Oven: Injector: Detector: 1. 2. 3. 4. 5. 6. 7.

DB-5
30 m x 0.32 mm id, 0.25 m 123-5032 Helium at 40 cm/sec, measured at 140C 140-320C at 12/min 320C for 4 min Split 1:75, 250C 1 L of 0.5 g/L each in methanol FID, 300C Nitrogen makeup gas at 30 mL/min 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11.

DB-5
20 m x 0.18 mm id, 0.4 m 121-50232 Helium at 39 cm/sec, measured at 100C 100-240C at 10/min Split 1:100, 250C 1 L of 2 g/L each in pyridine FID, 300C Nitrogen makeup gas at 30 mL/min Nicotine Unknown Acetylsalicylic acid (aspirin) Ibuprofen Acetaminophen Unknown Caffeine

Caffeine Ketamine Lidocaine Procaine Cocaine Codeine Morphine 6-Acetylcodeine Diacetylmorphone (Heroin) Quinine Strychnine

C135

C140

GC and GC/MS

Aspirin and Ibuprofen in Methanol


Column:
P/N: Carrier: Oven: Injector: Detector: 1. Aspirin 2. Ibuprofen

Free Steroids
Column:
P/N: Carrier: Oven:

DB-FFAP
30 m x 0.25 mm id, 0.25 m 122-3232 Hydrogen at 24 cm/sec, measured at 180C 180C isothermal Split 1:50, 250C FID, 300C Nitrogen makeup gas at 30 mL/min

DB-17
30 m x 0.25 mm id, 0.15 m 122-1731 Hydrogen at 44 cm/sec 260C isothermal Injector: Detector: Split 1:100, 1 L, 250C FID, 300C Nitrogen makeup gas at 30 mL/min

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12.

Coprostane (5--cholestane) 5--Androsterone 5--Cholestane Androsterone Epiandrosterone (trans-androsterone) 17--Estradiol -Estradiol Estrone Progesterone Cholesterol Estriol Stigmasterol

C139

C148

www.agilent.com/chem

421

GC and GC/MS Applications


Life Sciences
Anabolic Steroids
Column:
P/N: Carrier: Oven: Injector: Detector: 1. 2. 3. 4. 5. 6. 7. 8.

DB-1
30 m x 0.25 mm id, 0.1 m 122-1031 Helium at 40 cm/sec, measured at 180C 180-320C at 10/min 320C for 4 min Split 1:40, 2 L of 0.125 g/L each in methanol FID, Nitrogen makeup gas at 30 mL/min 11. 12. 13. 14. 15. 16. 17. Oxymetholone 19-Nortestosterone-17-propionate 4-Chlortestosterone-17-acetate (Clostebal) Stanozolol 1-Dehydrotestosterone benzoate 19-Nortestosterone-17-decanoate 1-Dehydrotestosterone undecylenate

9-THC (Marijuana) and Major Metabolites - TMS Derivatives


Column:
P/N: Carrier: Oven:

DB-5
30 m x 0.32 mm id, 0.25 m 123-5032 Helium at 40 cm/sec, measured at 100C 100C for 1 min 100-175C at 30/min 175-295C at 12/min Splitless, 250C 30 sec purge activation time 1 L of 0.1 g/L each in pyridine FID, 300C Nitrogen makeup gas at 30 mL/min

Dehydroisoandrosterone (Prasterone) 5-Androstan-17-ol-3-one (Stanolone) 19-Nortestosterone (Nandrolone) Mesterolone Testosterone 1-Dehydrotestosterone (Boldenone) 17-Methyltestosterone 1-Dehydro-17--methyltestosterone (Methandrostenolone) 9. Norethandrolone 10. 1-Dehydrotestosterone acetate

Injector:

Detector:

1. 9-THC 2. L-11-Hydroxy-9-THC 3. L-9-Carboxy-11-nor-9-THC

C147

C136

GC and GC/MS

Blood Alcohols (Static Headspace/Split)


Column:
P/N: Carrier: Oven: Injector: Detector:

Blood Alcohols (Static Headspace/Split)


Column:
P/N: Carrier: Oven: Injector: Detector:

DB-ALC1
30 m x 0.53 mm id, 3.0 m 125-9134 Helium at 80 cm/sec, measured at 40C 40C Isothermal Headspace to split inlet, Split 1:10, 250C FID, 300C Nitrogen makeup gas at 23 mL/min Headspace Conditions Oven: Loop: Transfer Line: Vial Equil. Time: Pressurization Time: Loop Fill Time: Loop Equil. Time: Inject Time: Sample Loop Size: Sample Composition: 70C 80C 90C 10 min 0.20 min 0.20 min 0.05 min 0.1 - 0.2 min 1.0 mL 0.1% Ethanol, 0.001% Others

DB-ALC2
30 m x 0.53 mm id, 2.0 m 125-9234 Helium at 80 cm/sec, measured at 40C 40C Isothermal Headspace to split inlet, Split 1:10, 250C FID, 300C Nitrogen makeup gas at 23 mL/min
3

1. 2. 3. 4. 5. 6.

Methanol Acetaldehyde Ethanol Isopropanol Acetone 1-Propanol

1. 2. 3. 4. 5. 6.

Methanol Acetaldehyde Ethanol Isopropanol Acetone 1-Propanol

Headspace Conditions Oven: Loop: Transfer Line: Vial Equil. Time: Pressurization Time: Loop Fill Time: Loop Equil. Time: Inject Time: Sample Loop Size: Sample Composition:

70C 80C 90C 10 min 0.20 min 0.20 min 0.05 min 0.1 - 0.2 min 1.0 mL 0.1% Ethanol, 0.001% Others

1 2

0.5

1.5 Time (min)

2.5

3.0

C670

C671

422

www.agilent.com/chem

GC and GC/MS Applications


Life Sciences
Blood Pollutants
Column:
P/N: Carrier: Oven: Injector: Detector:

DB-ALC1
30 m x 0.53 mm id, x 3.0 m 125-9134 Helium, 36 cm/sec, measured at 40C 40C for 5 min 40-210C at 10/min 250C, Split 1:10 FID, 300C

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16.

Methanol* Acetaldehyde* Ethanol* Diethyl ether Isopropyl alcohol* Methylene Chloride Acetone* Acetonitrile Ethyl formate t-Butyl alcohol* 1-Propanol MTBE Hexane Chloroform sec-Butyl alcohol 2-Chlorobutane

17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32.

MEK (2-Butanone) Ethyl acetate 1,1-Trichloroethane Carbon tetrachloride 1-Chlorobutane Benzene 1-Butanol Heptane Ethylene glycol soamyl alcohol Toluene Isopropyl amine (not shown) Ethylbenzene m,p-Xylene o-Xylene DMSO

C1072

GC and GC/MS

Blood Pollutants
Column:
P/N: Carrier: Oven: Injector: Detector:

DB-ALC2
30 m x 0.53 mm id, x 2.0 m 125-9234 Helium, 36 cm/sec, measured at 40C 40C for 5 min 40-210C at 10/min 250C, Split 1:10 FID, 300C

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16.

Methanol* Acetaldehyde* Ethanol* Diethyl ether Isopropyl alcohol* Methylene Chloride Acetone* Acetonitrile Ethyl formate t-Butyl alcohol* 1-Propanol MTBE Hexane Chloroform sec-Butyl alcohol 2-Chlorobutane

17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32.

MEK (2-Butanone) Ethyl acetate 1,1-Trichloroethane Carbon tetrachloride 1-Chlorobutane Benzene 1-Butanol Heptane Ethylene glycol soamyl alcohol Toluene Isopropyl amine (not shown) Ethylbenzene m,p-Xylene o-Xylene DMSO

C1073

www.agilent.com/chem

423

GC and GC/MS Applications


Life Sciences
Residual Solvents, USP <467>
Column:
P/N: Carrier: Oven: Injector: Detector:

DB-624
30 m x 0.53 mm id, 3.0 m 125-1334 Helium at 35 cm/sec, measured at 40C 40C for 20 min 40-90C at 5/min Megabore Direct, 250C 5 m phenylmethylsilane deactivated retention gap FID, 300C Nitrogen makeup gas at 30 mL/min

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12.

Methanol Ethanol Ethyl ether Acetone Isopropanol Acetonitrile Methylene chloride tert-Butanol n-Hexane n-Propanol Methyl ethyl ketone (MEK) Ethyl acetate

13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24.

Tetrahydrofuran (THF) Chloroform sec-Butanol Cyclohexane Benzene n-Heptane Trichloroethylene n-Butanol 1,4-Dioxane Pyridine Toluene Dimethylformamide (DMF)

C144

GC and GC/MS

Fast GC Analysis of USP Solvents


Column:
P/N: GC: Carrier: Oven:

Can't find what you're looking for?


Save time and avoid trial and error by accessing pre-defined, high-quality applications. Consult Agilent's online, searchable Chromatogram Database
Containing hundreds of commonly analyzed compounds, this resource helps chromatographers select the right analytical technique, column and conditions for an application. Search the extensive database for GC, LC and CE applications by compound name or CAS number.

HP-Fast GC Residual Solvent Column


30 m x .53 mm id, 1.0 m 19095V-420 6890 Helium, 30 cm/min, constant flow 40C for 1.7 min 40-90C at 6C/min 90-250C at 30C min Headspace, 180C, Split 7/1 FID, 260C

Injector: Detector:

1. 2. 3. 4. 5.
3

Methylene Chloride (25 ppm) Chloroform (12 ppm) Benzene (25 ppm) Trichloroethylene (25 ppm) 1, 4-Dioxane (25 ppm)

4 2

To access the Chromatogram Database go to www.agilent.com/chem and click on "Library."


6

4 Time (min)

C843

424

www.agilent.com/chem

GC and GC/MS Applications


Petroleum

Petroleum
Noble Gases
Column:
P/N: GC: Carrier: Oven:

Permanent Gases
Column:
P/N: GC: Carrier: Oven: Sample: Detector:

HP-PLOT/MoleSieve
30 m x 0.53 mm i.d., x 50 m 19095P-MS0 6890 Helium, 4 mL/min 35C for 3 min 35-120C at 25C/min 120C for 5 min 250 L, split (50:1) TCD 4 2 5 6

HP-PLOT/MoleSieve
30 m x 0.32 mm i.d., 12 m 19091P-MS4 6890 Helium, 2 mL/min 40C Isothermal 250 L, split (75:1) TCD

Sample: Detector:

1. 2. 3. 4. 5. 6.

Neon Argon Oxygen Nitrogen Krypton Xenon

1. 2. 3. 4. 5. 6.

Neon Argon Oxygen Nitrogen Methane Carbon monoxide

5 3 1 2

GC and GC/MS

2 Time (min) C887

C886

4 Time (min)

Baseline Resolution of Air/CO, CO2, and Methane in a Natural Gas Sample


Column:
P/N: GC: Carrier: Oven:

Natural Gas
Column:
P/N: Carrier: Oven: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. Air/CO CO2 Ethane H2S Water C3 i-C4/n-C4 neo-C5 i-C5/n-C5 C6 C7 Injection: Sample: Detector:

HP-PLOT Q
30 m x 0.53 mm i.d., 40 m 19095P-Q04 6890 Helium (8.6 mL/min @ 60C) 60C for 2 min 60-240C at 30C/min 240C for 1 min Split mode (12:1) 0.25 cc natural gas sample, Methane, 80%+ TCD 250C

HP-PLOT/Al2O3 S
15 m x 0.53 mm i.d., 15 m 19095P-S21 Helium, 50 cm/sec (100C), 6 mL/min 100C for 1.5 min 100-180C at 30C/min Split (50:1), 5 L, Inlet 250C Natural gas P/N 5080-8756 FID 250C

Injection: Sample: Detector:

1. 2. 3. 4. 5. 6. 7. 8.

Methane Ethane Propane iso-Butane n-Butane iso-Pentane n-Pentane n-Hexane

1 23

45

1 2

6 5 67 8 4 8 9 10 11 0
C860

4 Time (min)

8
C859

2 Time (min)

www.agilent.com/chem

425

GC and GC/MS Applications


Petroleum
Ethylene
Column:
P/N: GC: Sample: Carrier: Oven: Injection: Detector: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. Methane Ethane Ethylene Propane Cyclopropane Propylene Isobutane n-Butane Propadiene Acetylene trans-2-Butene Butene-1 Isobutylene cis-2-Butene Isopentane n-Pentane 1,3-Butadiene Propyne trans-2-Pentene 2-Methyl-2-butene Pentene-1 cis-2-Pentene n-Hexane

HP- PLOT/Al2O3 S
50 m x 0. 53 mm i.d., 15 m 19095P-S25 6890 Ethylene 98.4% Helium, 50 cm/sec (35C), 7 mL/min constant flow 35C for 2 min 35-100C at 5C/min Split 65:1, 250C, 5 L FID 250C

1 2

11 12 14 16

17

13 15 18 19 20 10 9 21 22 23 10 Time (min) 15 20 25

GC and GC/MS

5 0
C885

Impurities in Ethylene
Column:
P/N: Carrier: Oven:

GS-Alumina/KCl
50 m x 0.53 mm i.d. 115-3352 Helium at 8 mL/min, measured at 35C 35C for 2 min 35-190C at 10/min 190C for 3 min Split 1:40, 200C 0.2 mL of trace hydrocarbons in ethylene FID, 200C Nitrogen makeup gas at 20 mL/min

Injector:

Detector:

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19.

Methane Ethane Ethylene Propane Cyclopropane Propylene Isobutane Acetylene n-Butane Propadiene trans-2-Butene 1-Butene Isobutylene cis-2-Butene Isopentane n-Pentane Propyne 1,3-Butadiene 1-Pentene

C479

426

www.agilent.com/chem

GC and GC/MS Applications


Petroleum
Impurities in Propylene
Column:
P/N: Carrier: Oven: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. Methane Ethane Ethylene Propane Cyclopropane Propylene Isobutane Acetylene n-Butane Propadiene trans-2-Butene 1-Butene Isobutylene cis-2-Butene Isopentane n-Pentane Propyne 1,3-Butadiene 1-Pentene

GS-Alumina/KCl
50 m x 0.53 mm i.d. 115-3552 Helium at 10 mL/min, measured at 35C 35C for 2 min 35-190C at 10/min 190C for 3 min Split 1:30, 200C 0.2 mL of trace hydrocarbons in propylene FID, 200C Nitrogen makeup gas at 20 mL/min

Injector:

Detector:

C480

GC and GC/MS

Propylene
Column:
P/N: Carrier: Oven:

GS-Alumina
50 m x 0.53 mm i.d. 115-3552 Helium at 10 mL/min, measured at 35C 35C for 2 min 35-190C at 10/min 190C for 3 min Split 1:30, 200C 0.2 mL of trace hydrocarbons in propylene FID, 200C Nitrogen makeup gas at 20 mL/min

Injector:

Detector:

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20.

Methane Ethane Ethylene Propane Cyclopropane Propylene Isobutane Acetylene n-Butane Propadiene trans-2-Butene 1-Butene Isobutylene cis-2-Butene Isopentane n-Pentane Propyne 1,3-Butadiene 1-Pentene n-Hexane

C586

www.agilent.com/chem

427

GC and GC/MS Applications


Petroleum
Propylene, Butene-1 and Ethylene
Column:
P/N: Carrier: Oven: Injector: Detector: Propylene 1. Methane 2. Ethylene 3. Propylene 4. Propane

DB-Petro100
100 m x 0.25 mm i.d., 0.5 m 122-10A6 Helium at 28.5 cm/sec -40C for 20 min -40-120C at 5/min Split 1:16, 100 L, 200C FID, 250C Nitrogen makeup gas at 30 mL/min

Butene-1 1. Isobutane 2. Butene-1 3. 1,3-Butadiene 4. n-Butane 5. trans-2-Butene 6. cis-2-Butene

Ethylene 1. Methane 2. Ethylene 3. Ethane 4. Propylene

C154

1,3-Butadiene

GC and GC/MS

Column:
P/N: Carrier: Oven:

DB-624
25 m x 0.20 mm i.d., 1.12 m 128-1324 Helium at 1.0 mL/min -20C for 3 min -20 - 20C at 4/min 20 - 200C at 8/min 200C for 10 min Split 1:150, 250C 0.5 L injection FID, 250C

Injector: Detector:

Agilent Technologies wishes to thank DCG Industries (Pearland, TX) for providing this chromatogram. 8,9 11 12 2,3 13

7 10

Refined Butadiene Standard Component 1. Acetylene 2. Propane 3. Propylene 4. Propadiene (allene) 5. Propyne (methylacetylene) 6. Cyclopropane 7. Isobutane 8. Butene-1 9. Isobutylene 10. n-Butane 11. 1,3-Butadiene 12. trans-2-Butene 13. cis-2-Butene 14. 1-Butyne (ethylacetylene) 15. 1,2-Butadiene 16. 3-Methyl-1-butene 17. Isopentane 18. Pentene-1 19. n-Pentane 20. 2-Butyne (dimethylacetylene) 21. trans-2-Pentene 22. Isoprene 23. cis-2-Pentene 24. trans-1,3-Pentadiene 25. cis-1,3-Pentadiene 26. Benzene 27. Toluene 28. Dimer (4-vinylcyclohexene-1)

Gravimetric concentration (PPM) 20.7 19.8 296 21.1 21.0 20.0 506 999 495 494 balance 442 1946 20.2 28.9 19.8 50.1 29.8 50.1 150 5.57 20.0 13.9 13.8 7.73 20.3 20.2

28

20

5 4

6 14
5 5 75

15 16

17

19 18
10 10

22 21 23
12 5

24

26 25
15 15 17 5 20 20

27

0 C777

25

22 5

Time (min)

428

www.agilent.com/chem

GC and GC/MS Applications


Petroleum
1,3-Butadiene Purity
Column:
P/N: Carrier: Oven: Refined Butadiene Standard Component Gravimetric concentration (PPM) 1. Acetylene 20.7 2. Propane 19.8 3. Propylene 296 4. Propadiene (allene) 21.1 5. Propyne (methylacetylene) 21.0 6. Cyclopropane 20.0 7. Isobutane 506 8. Butene-1 999 9. Isobutylene 495 10. n-Butane 494 11. 1,3-Butadiene balance 12. trans-2-Butene 442 13. cis-2-Butene 1946 14. 1-Butyne (ethylacetylene) 20.2 15. 1,2-Butadiene 28.9 16. 3-Methyl-1-butene 19.8 17. Isopentane 50.1 18. Pentene-1 29.8 19. n-Pentane 50.1 20. 2-Butyne (dimethylacetylene) 150 21. trans-2-Pentene 5.57 22. Isoprene 20.0 23. cis-2-Pentene 13.9 24. trans-1,3-Pentadiene 13.8 25. cis-1,3-Pentadiene 7.73 26. Benzene 20.3 27. Toluene 20.2 28. Dimer (4-vinylcyclohexene-1) 5 18

GS-Alumina
50 m x 0.53 mm i.d. 115-3552 Helium, 6.0 mL/min (constant flow mode) 45C for 3 min 6/min to 195C 195C for 15 min 250C, Split 1:50 0.5 L injection FID, 250C

Injector: Detector:

12

13 11

15,19

17

GC and GC/MS

23 16 21

13

14

15

16 Time (min)

17

18

19

20 28

3 7 10

20

14 2 6 4 1 n-C6 24 25 22 26 27

5 C798

10

15

20 Time (min)

25

30

35

www.agilent.com/chem

429

GC and GC/MS Applications


Petroleum
Extended Hydrocarbon Analysis on GS-Alumina and GS-GasPro
Column: GS-Alumina
30 m x 0.53 mm i.d. P/N: 115-3532 Carrier: Helium at 52 cm/sec (6.7 mL/min), measured at 100C Oven: 100C for 1 min 100-140C at 8/min 140C for 0.5 min 140-200C at 30/min Injector: Split 1:8, 250C 300 L injection of 100 ppm V SUMMA cannister mixture Detector: FID, 275C Nitrogen makeup gas at 29 mL/min (not all peaks shown ) 1. Methane 2. Ethane 3. Ethylene 4. Propane 5. Propylene 6. Isobutane 7. Acetylene 8. n-Butane 9. trans-2-Butene 10. 1-Butene 11. cis-2-Butene 12. Cyclopentane 13. Isopentane 14. n-Pentane 15. Propyne 16. 1,3-Butadiene 17. Cyclopentene 18. 3-Methyl-1-butene 19. trans-2-Pentene 20. 2-Methyl-2-butene 21. 1-Pentene 22. cis-2-Pentene 23. Methylcyclopentane 24. 2,2-Dimethylbutane 25. Cyclohexane 26. 2,3-Dimethylbutane

Enlarged view of peaks 15-30

GC and GC/MS

C608

Column:
P/N: Carrier: Oven:

GS-GasPro
60 m x 0.32 mm i.d. 113-4362 Helium at 40 cm/sec (3.3 mL/min), measured at 80C 80C for 0.5 min 80-175C at 25/min 175C for 2 min 175-250C at 25/min Split 1:17, 250C 500 L injection of 100 ppmV SUMMA cannister mixture FID, 275C Nitrogen makeup gas at 32 mL/min

Enlarged view of peaks 18-40

Injector:

Detector:

27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38. 39. 40. 41. 42. 43. 44. 45. 46. 47. 48. 49. 50. 51. 52. 53. 54. 55. 56. 57.

2-Methylpentane 3-Methylpentane Isoprene n-Hexane 4-Methyl-1-pentene trans-2-Hexene 2-Methyl-1-pentene cis-2-Hexene 2,4-Dimethylpentane Methylcyclohexane 2,3-Dimethylpentane 2-Methylhexane 3-Methylhexane n-Heptane Benzene Isooctane (2,2,4-trimethylpentane) 2,3,4-Trimethylpentane 3-Methylheptane 2-Methylheptane n-Octane Toluene n-Nonane Ethylbenzene m-Xylene p-Xylene o-Xylene Styrene Isopropylbenzene (cumene) n-Propylbenzene 1,3,5-Trimethylbenzene 1,2,4-Trimethylbenzene

430

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GC and GC/MS Applications


Petroleum
Separation of Refinery Gas Sample
Column:
P/N: GC: Carrier: Oven:

Refinery Gas
Column:
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. Air/CO C1 CO2 Ethylene C2 H2O COS H2O Propylene C3 MeOH i-C4 t-C4 n-C4 cis-C4 i-C5 n-C5 P/N: GC: Carrier: Oven: Injection: Split: Detector:

HP-PLOT Q
0.53 mm x 30 m i.d., 40 m 19095P-Q04 6890 Helium p=9.0 psi @ 60C 60C for 5 min 60-200C at 20C/min 200C for 1 min 250C Split mode 0.25 cc valve Refinery gas and others 100 mL/min TCD 250C

HP-PLOT/AI203 S
50 m x 0.53 mm i.d., 15 m 19095P-S25 6890 Helium 7 mL/min 100C Isothermal Split (100:1), inlet 250C, 5 L 100 mL/min FID 250C 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. Methane Ethane Ethylene Propane Propylene iso-Butane n-Butane trans-2-Butene 1-Butene cis-2 Butene iso-Pentane n-Pentane

Inlet: Sample: Split flow: Detector:

1 23 5

2 6

13 14 11 12 4 10 6 7 8 9 16 17 3 5 15 1 4

9 8

10 11

GC and GC/MS

12 12

0
C862

6 Time (min)

10

12

0
C863

6 Time (min)

10

Refinery Gas
Column:
P/N: Carrier: Oven:

PONA
50 m x 0.2 mm i.d., 0.5 m 19091S-001 Helium, 1 mL/min 35C for 3 min 35-100C at 10C/min 100C for 5 min Split (200 -400:1), 2 cc loop FID 250C 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. C1 C2, C2= C3= C3 IC4 NC4 1-Butene/t-2-butene cis-2-Butene IC5 NC5

Injection: Detector:

5 67 8

10

3
C864

4 Time (min)

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431

GC and GC/MS Applications


Petroleum
Volatile Sulfur Compounds
Column:
P/N: Carrier: Oven: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. Hydrogen sulfide Carbonyl sulfide Methyl mercaptan Ethyl mercaptan Dimethyl sulfide Carbon disulfide 2-Propanethiol 2-Methyl-2-propanethiol 1-Propanethiol Ethyl methyl sulfide Thiophene 2-Methyl-1-propanethiol 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. Diethyl sulfide 1-Butanethiol Methyl disulfide 2-Methylthiophene 3-Methylthiophene Tetrahydrothiophene 1-Pentanethiol 2-Ethylthiophene 2,5-Dimethylthiophene 1-Hexanethiol Ethyl disulfide 1-Heptanethiol

DB-1
30 m x 0.32 mm i.d., 5.0 m 123-1035 Helium at 23 cm/sec (H2S at 50C) 50C for 4 min, 50-120C at 20/min, 120C for 4 min, 120-220C at 25/min, 220C for 2.5 min Split 1:10, 200C, 600 L of sulfur gas standard, 3 ppmV each component PFPD (OI Analytical), 220C

Injector: Detector:

Agilent wishes to thank Air Toxics, Ltd. (Folsom, CA) for providing the standard mixture shown in this chromatogram.

GC and GC/MS

C775

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432

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GC and GC/MS Applications


Petroleum
Sulfur Gas Analysis in Light Hydrocarbon Streams
Column:
P/N: Injector: Carrier: Oven: Detector: Sulfur compounds (PFPD) A. Carbonyl sulfide B. Hydrogen sulfide C. Sulfur dioxide D. Carbon disulfide E. Methyl mercaptan F. Ethyl mercaptan Hydrocarbons (FID) 1. Methane 2. Ethane 3. Ethylene 4. Acetylene 5. Propane 6. Propylene 7. iso-Butane 8. n-Butane 9. 1-Butene/Methyl acetylene 10. trans-2-Butene 11. 1,3-Butadiene 12. cis-2-Butene 13. iso-Pentane 14. n-Pentane

GS-GasPro
30 m x 0.32 mm i.d. 113-4332 200C, 1:20 split Helium, 10 psig, 2.0 mL/min @ 60C 60C for 2 min, 20/min to 260C and hold Two separate analyses under identical conditions on FID and PFPD

PFPD

Time (min)

10

FID
1

13 14 12

7 8 5 2
C800

10 11

GC and GC/MS

Sulfur Gas Analysis in Light Hydrocarbon Streams


Column:
P/N: Injector: Carrier: Oven: Detector:

GS-Q
30 m x 0.32 mm i.d. 113-3432 200C, 1:20 split Helium, 10 psig, 1.7 mL/min @ 100C 100C for 2 min, 20/min to 250C and hold Two separate analyses under identical conditions on FID and PFPD

Sulfur compounds (PFPD) A. Hydrogen sulfide B. Carbonyl sulfide C. Methyl mercaptan D. Ethyl mercaptan E. Carbon disulfide

PFPD

Hydrocarbons (FID) 1. Methane 2. Ethylene/Acetylene 3. Ethane 4. Propylene 5. Propane 6. Methyl acetylene 7. iso-Butane 8. 1-Butene 9. 1,3-Butadiene 10. n-Butane/cis-2-Butene 11. trans-2-Butene 12. iso-Pentane 13. n-Pentane

Time (min)

12

FID
4 1 5 6

7 13 11

9 10
C799

3 2

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433

GC and GC/MS Applications


Petroleum
Sulfur Compounds in Propylene (1 ppm)
Column:
P/N: Oven: Injector: Sample: Detector: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. COS H2 S Propylene CS2 Methyl mercaptan Ethyl mercaptan Thiophene Dimethyl sulfide 2-Propanethiol 1-Propanethiol 2-Methyl-2-propanethiol 2-Methyl-1-propanethiol 1-Methyl-1-propnaethiol 1-Butanethiol

Mercaptans
Column:
P/N: Carrier: Oven: Injector: Detector:

GS-GasPro
30 m x 0.32 mm i.d. 113-4332 60C for 2.5 minutes 60-250C at 10C/min OI Analytical Volatiles Inlet 5:1 split, 200 L gas sampling valve 1 ppm Sulfur compounds in Propylene OI Analytical Model 5380 PFPD

GS-GasPro
30 m x 0.32 mm i.d. 113-4332 Helium at 25 cm/sec 175C for 2 min 175-260C at 10/min Split, 80 mL/min split flow 0.2 mL 3 FID

1. 2. 3. 4. 5. 6. 7. 8. 9.

Ethyl mercaptan 2-Propyl mercaptan 1-Propyl mercaptan 2-Methyl-2-propyl mercaptan 2-Methyl-1propyl mercaptan 1-Methyl-1-propyl mercaptan 1-Butyl mercaptan 1-Pentyl mercaptan 1-Hexyl mercaptan

1 2

1 1 5 6 4 4 4
2 2

8 9

6 6 5 5

8 7 7 9 9

10 10

13 14 13

14

11 11 12 12 3 3

GC and GC/MS

C801 Chromatogram courtesy of OI Analytical.

Time (min)

0
C530

Sulfur Compounds in Natural Gas-Synthetic Mixture


Column:
P/N: Carrier: Oven: Injection: Detector:

Sulfur Compounds in Naphtha


Column:
P/N: Carrier: Oven:

HP-1
50 m x 0.2 mm i.d., 0.5 m 19091Z-205 Helium 35C for 10 min 35-300C at 7C/min Split (100:1) 0.5 mL FPD

3 12 45 7 8 9 11 12 10 13

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16.

Hydrogen sulfide Methyl mercaptan Ethyl mercaptan Dimethyl sulfide Isopropyl mercaptan tert-Butyl mercaptan n-Propyl mercaptan Thiophene and sec-Butyl mercaptan Isobutyl mercaptan n-Butyl mercaptan tert-Amyl mercaptan Isoamyl mercaptan n-Amyl mercaptan n-Hexyl mercaptan tert-Dibutyl disulfide n-Octyl mercaptan

PONA
50 m x 0.2 mm i.d., 0.5 m 19091S-001 Helium, 26 cm/sec 35C for 15 min 35-70C at 8C/min 70-130C at 15C/min Split (400:1), 3 L FPD 1. 2. 3. 4. Thiophene Methyl thiophenes Ethyl and dimethyl thiophenes Benzothiophene

Injection: Detector:

16

14 15 6

C870

30 min

C868

42 min

434

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GC and GC/MS Applications


Petroleum
ASTM D16 Analytes- Aromatics Analysis
Column: HP-INNOWax
P/N: Carrier: Oven: 60 m x 0.32 mm i.d., 0.5 m 19091N-216 Helium at 20 psi, constant pressure mode 75C for 10 min 3C/min to 100C 10C/min to 145C 250C, Split/Splitless 100:1 to 400:1 split ratio 250C, FID Data aquisition rate at 20 Hz 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. Heptane Cyclohexane Octane Nonane Benzene Decane Toluene 1-4-Dioxane Undecane Ethylbenzene p-Xylene m-Xylene Cumene Dodecane 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. o-Xylene Propylebenzene p-Ethyltoluene m-Ethyltoluene tert-Butylbenzene sec-Butylbenzene Styrene Tridecane Diethylbenzene isomer Diethylbenzene isomer n-Butylbenzene -Methylstyrene Phenylacetylene

Injector: Detector:

1 23

6 7 25 9 10 11 12 13 14 15 16 18 17 19 20 21 22 23 27 26

GC and GC/