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An overview of quantum dot synthesis, applications and safety

Nastassja Lewinski Department of Bioengineering nal@rice.edu

What are Quantum Dots?


III IV V VI

II II-VI: ZnS, ZnSe, CdSe, CdTe, CdS, HgS III-V: GaAs, GaN, InP, InAs, InGaAs IV-VI: PbS, PbSe

What do Quantum Dots look like?

Control

AntiHER2

Quantum dots ~2 - 6 nm

How are they made?

Synthesis Movie

http://www.youtube.com/watch?v=2XfFQKEfCcw&feature=related

Why do we care?

Medintz et al. Nature Mat., 4, 2005.

Energy

Luminescence

Absorption (excitation)

Fluorescence (emission)

Excited State

Ground State

Electromagnetic Spectrum

400 nm

700 nm

Bare CdSe QD Spectra

CdSe/ZnS Excitation-Emission Matrix

Water solubilization
Ligand exchange
HS O OH

Mercaptoundecanoic acid

Polymer wrapping
COOH COOH CH3 -[CH2-CH]x-[CH2-CH]y-[CH2-C-CH2-C-CH2-C]zCONHC8H17 COOC4H9 Amphiphilic triblock polymer COOC2H5

-(CH-CH-CH2-CH)mO OH O NH (O-CH2-CH2)n-OCH3

Silica shelling
O

Polymaleic anhydride oxide - polyethylene glycol (PMAO-PEG)

O Si O O

O n
O Si NH2

O Si SH

2-[methoxy(polyethleneoxy)propyl]O trimethoxysilane (PEOS) 3-aminopropylmethyldimethoxysilane 3-mercaptopropylmethyldimethoxysilane (APS) (MPS)

Particle Aggregation

How are they used?

Sapsford et al. Sensors, 6, 2006

Parungo et al. JTCS, 129, 2005

Green, Angew. Chem., 43, 2004

Gao et al. Nat.Biotech., 22, 2004 Michalet et al. Science, 307, 2005

Tumor Tissue Margin Detection

NIR Optical Window

Absorption coefficient (cm-1)

Wavelength (nm)

Toxicity Concerns
O HS OH

Low pH - lysosomes Oxidation - peroxisomes

-(CH-CH-CH2-CH)mO OH O NH (O-CH2-CH2)n-OCH3

Lewinski et al. Small, 4, 2008

Daphnia magna

4x Brightfield

Qdot Localization
Rectum 10x Brightfield

10x Fluorescence (DAPI excitation filter)

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