food products Philippe Mongondry p.mongondry@groupe-esa.com 29 June 2011, Maribor 2 Many analytical chemical and physical techniques:
Electro-analysis Q ty of ions Gravimetry Fat or water content Electrophoresis separation Atomic spectrometry trace element Molecular spectrometry emission (Fluo); diffusion (Raman) absorption (UV, IR RMN) Mass Spectrometry Radiometry ( 14 C) origin & date X ray diffusion structural ID Rheometry Flow & structure Sensory analysis Chromatography
3 Techniques for food products: what for?
Quality control
Quantification of nutriments, vitamins of compounds Identification of contamination Determination of ingredients
Authentication of origin of the product
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Physical and chemical analysis Spectroscopy (UV visible IR) Chromatography SNIF NMR and Mass spectrometry
Concepts and Basis Sensory analysis 5 Matter Spectroscopy and interactions 6 From atom to complex molecules 7 Biological Molecules Thousands of different molecules per cell, fast Renew Complex system Biological specificity with 'allowed' movement and conformation E(link etc...)reactions, biological and thermodynamic parameters Particularity : transformation of light energy (chlorophyll) Lipids Glucide Proteins Nucleic acid 8 electromagnetic radiance domain Phenomena Frequency (Hz) Wave length Radiofrequency Nuclear Magnetic Resonance Nuclear Quadrupole Resonance 10 6 to 10 8 300 to 3 m Micro-ondes Electronic paramagnetic Resonance molecular rotations spectrum 10 10 to 10 12 300 to 0.3 mm Infrared molecular vibrations 10 12 to 310 14 300 to 0,8 m Visible and ultraviolet Electronic Transitions (external e-) 310 14 to 10 16 800 to 10 nm X Rays Electronic Transitions (core e-) 310 16 to 10 19 10 to 0.03 nm Rays nuclear Transitions 10 19 to 10 22
0.03 to 0.003 nm Spectroscopic principles 9 Powerful photons No! but right ones Yes! Transmission and reflection = nothing Spectroscopic principles Absorption of energy UV absorption spectrum Emission of large spectrum of photons (energy) 10
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Large spectre of the source Spectrale composition of transmise light Polychromatic radiation Abs Construction of absorption spectrum Spectroscopic principles 11 Spectroscopy UV-visible Quantitative method, Kinetic of reaction Absorption of UV by unsaturated bonds O O H NH 2 O O NO YES YES YES 12 Principe: Molecule under infrared radiation: vibration and rotation Symmetrical stretching Antisymmetrical stretching Scissoring Rocking Wagging Twisting http://fr.wikipedia.org/wiki/Spectroscopie_infrarouge Infrared Spectroscopy 13 Fourier Transform InfraRed Spectroscopy Fingerprint of molecule spectrometer FTIR Used for samples : Gaz, liquid or solid in powder 14 Separation & Identification Chromatography or HPLC Extraction and preparation in liquid media 15 Chromatography or HPLC High Performance Liquid Chromatography Different Types Partition chromatography Normal-phase chromatography Reversed-phase chromatography (RPC) Size-exclusion chromatography (SEC) Ion-exchange chromatography Bio-affinity chromatography SEC 16 Chromatogram of pesticide in cow milk Identification of polyphenols in wine 17 SNIF NMR Site-Specific Natural Isotope Fractionation Nuclear Magnetic Resonance Based on the absorption of deuterium in plants (Glucose or ethanol) Irregular distribution of De on the Earth due to rotation
Addition of different type of sugar in Tequilas 18 Mass Spectrometry Main steps of the measurement Unknown sample Determination of the molecular weight of big molecules (proteins)
Quick Identification based on databases Aims 19 Identification Reconstruction by affinity of pieces Depend on position of the picks and ratio m/Z Mass Spectrometry Coupling HPLC-MS Coupling techniques is powerful !! 20 Sensory analysis What consumer understand from different information we give him ? What consumer looks for ? How improve his loyalty ? How his sensory experiments is influenced by the information he has ? What is important in his purchase behavior ? What does he discern in products ? How to measure the sensory performance of products ? What is the impact of a new process or technology on his fondness ? 21 Sensory analysis How objectively measure the tasting and the sensorial characteristics of food products?
Habits Culture Personality Current mood Physiology (allergy) Time of the day place With who how Why what Sensorial characteristics Nutriments Packaging Factors which influence the choice of a food product ( Gains, 1994) Consumer Context product 22 Definition:
Evaluation of organoleptic properties of a food product by human senses based on experimental design and statistical analysis (combination of inferences or deductive reasoning and insight or specific cause and effect) Sensory analysis of Food products ICS 67.240 Sensory analysis (ISO standard) Glossary 23 Sensory analysis How objectively measure the tasting and the sensorial characteristics of food products?
differences preferences Evocations Motivations 3 categories: Effective, affective and perception analysis Descriptive Test Hedonic Test Qualitative Test Trained panel Naive consumers 24 Study consumer behavior and motivation to get a better understanding of his reactions Sensory analysis Aims: Identify all reactions, all opinions that consumer have Example : Reaction in front of the product Label, design Information Price Association of these different attributes Which cap do you prefer ?? 25 Thank you for your attention It is time for sensory analysis