Set No:

Code No: 211101

II B.Tech. I-Semester Supplementary Examinations, November 2003
BIO-CHEMISTRY
1
( Bio-Medical Engineering )
Time: 3 hours Max.Marks:80
Answer any FIVE questions
All questions carry equal marks
---

1. a) Explain the terms:

i) Specific Conductance and ii) Equivalent conductance.
b) Describe an experimental method to determine the equivalent conductance of an
electrolyte.

2. a) Explain the difference between addition and condensation polymerization with

examples.
b) What is polyethylene? How is it made? Mention the different grades of polythene
and their uses.

3. Write notes on:

a) Aerobic and anaerobic processes in connection with Sewage.
b) Analysis of Sewage.

4. a) What do you understand by the term hardness’ of a sample of water? Define the
degree of hardness and discuss the various units of its expression.
b) Distinguish between carbonate hardness and non-carbonate hardness of a sample
of water.
c) Calculate the temporary and permanent hardness of water, in ppm units,
Which analysis as follows:

Magnesium bicarbonate … 73 mg/L

Calcium bicarbonate … 162 mg/L
Calcium sulphate … 136 mg/L
Magnesium Chloride … 95 mg/L
Calcium Chloride … 111 mg/L
Sodium Chloride … 100 mg/L

5. a) Draw a neat diagram of generalized cell and label the different organelles.
b) Describe the organelles in an animal cell and explain the function of each
organelle.

6. a) What is the chemical nature of enzymes? And how are they isolated?
b) Explain the specificity of enzymes with suitable examples.
 (Contd…2)
Code No: 211101 .. 2.. Set No: 1

7. a) Illustrate the important steps involved in biosynthesis of starch.

b) What are the enzymes and their mode of action on degradation of starch?
c) Show important difference between starch and cellulose.

8. Describe the haemoglobin buffer system and add a note on plasma protein
buffering action.

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 Set No:
Code No: 211101
II B.Tech. I-Semester Supplementary Examinations, November 2003
BIO-CHEMISTRY
2
( Bio-Medical Engineering )
Time: 3 hours Max.Marks:80
Answer any FIVE questions
All questions carry equal marks
---

1. a) Explain the construction of:

i) A hydrogen electrode. ii) A calomel electrode.
b) Describe the Kohlrausch’s law of independent migration of ions.

2. Describe the preparation, properties and uses of:

a) Bakelite.
b) PVC.

3. a) Name the impurities present in natural waters.

b) Explain the function of the following in water treatment.
(i) Calgan, (ii) Bleaching powder, (iii) Ferricalum

4. a) Write the structure of EDTA. Discuss the principle of the EDTA titration for the
determination of hardness of water and describe the experimental procedure for
the same.
b) 50 ml. of standard hard water containing 1 mg of pure calcium carbonate per ml,
consumed 20 ml of EDTA in the titration using eriochome – black – T indicator.
50 ml. of hard water sample required. 25 ml of the same EDTA. 50 ml. of the
same hard water sample after boiling and filtering consumed 18 ml of EDTA.
Calculate the temporary and permanent hardness of water sample.

5. a) Define a living cell and draw a neat diagram of a plant cell along with the
organelles.
b) How the fractionation of cell organelles is carried out by centrifugation method?

6. a) How an enzyme is extracted and purified from a living cell?

b) Define the unit of enzyme activity. How can you explain the increase of
purification of an enzyme?

7. a) Illustrate and explain the structure and function of the synaptic function.
b) Write briefly on
i) Action potential
ii) Fatigue.

8. Explain how blood biochemical analysis helps in confirmation of diseases

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 Set No:
Code No: 211101
II B.Tech. I-Semester Supplementary Examinations, November 2003
BIO-CHEMISTRY
3
( Bio-Medical Engineering )
Time: 3 hours Max.Marks:80
Answer any FIVE questions
All questions carry equal marks
---

1. a) What is Arrhenius theory of electrolyte dissociation?

b) Describe the construction and mode of working of a Galvanic cell.

2. a) What is the Composition of natural rubber? How is it obtained from its natural
source?
b) Write a note on vulcanization of rubber.

3. a) Write short notes on (i) BOD, (ii) COD, (iii) break point chlorination.

4. a) Describe the lime soda process of water softening giving pertinent chemical
equations. Give the advantages and disadvantages of the process.
b) Calculate the amounts of lime and soda required in Kgs. for softening one lakh
litres of water with the following analysis.

Calcium bicarbonate : 8.1 mg/L.

Magnesium bicarbonate : 7.3 mg/L.
Calcium Sulphate : 13.6 mg/L.
Magnesium Sulphate : 12 mg/L.
Sodium Chloride : 4.6 mg/L.

5. a) How many types of living cells are there? Mention them. Describe the animal cell
with a neat diagram.
b) Explain the function of biological membrane in transportation of substances.

6. a) How the enzymes are classified? Explain different types of enzymes with one
example for each.
b) Explain the properties of an enzyme.

7. Give an account of transport of substances across biological membranes.

8. Briefly describe urine chemistry and explain the terms renal clearance, threshold
substances.

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 Set No:
Code No: 211101
II B.Tech. I-Semester Supplementary Examinations, November 2003
BIO-CHEMISTRY
4
( Bio-Medical Engineering )
Time: 3 hours Max.Marks:80
Answer any FIVE questions
All questions carry equal marks
---

1. a) What are electrode potentials? How are they useful?

b) Write briefly on the glass electrode.
o o
c) If ECu + 2 = 0.34 Volt and E Fe =0.4 Volt, Calculate the standard emf for
Cu Fe + 2
the cell, Fe/Fe+2 | | Cu+2/Cu.

2. a) Describe the preparation, properties and uses of Teflon.

b) What is PVC? Explain the difference between plasticized PVC and un plasticised
PVC. How are they manufactured?

3. Distinguish between:
(i) Flash point and cloud point.
(ii) Petroluem and synthetic lubricants.
(iii) Fluid film and boundary lubrcation

4. a) What are zeolites? How do they function in removing hardness of water? What
are the limitations of the process?
b) The hardness of one lakh litres of water is completely removed by passing
through a zeolite softner. The system is regenerated by 400 ml. of Sodium
chloride solution containing 100 g/L of Sodium Chlordie. Calculate the hardness
of the water, softened.

5. a) What are prokaryotes and eukaryotes? What are the differences between them?
And give suitable examples.
b) Explain the composition of cell wall of all living cells.

6. a) What is meant by ‘Km’ value? Explain its significance.

b) How the spectrophotometer is used to study enzyme Kinetics?

7. Write short notes on

a) Essential fatty acids and cholesterol.
b) Lipoproteins and triglycerides.

8. Explain how plasma proteins contribute for maintaining acid base balance and
add a note on the biochemical measurements of acid base status.

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