Table Index of Contents

Column Selection
SPE Phase Selection ...........................................................2 HPLC and LC/MS Column Selection ...................................4 GC Column Selection ........................................................17

SPE
HyperSep SPE Products ....................................................30 Finnpipettes.......................................................................46

29

GC and GC/MS
TRACE GC Capillary Columns ...........................................48 Ultra Fast GC Columns......................................................65

47

HPLC and LC/MS

Column Protection.............................................................66 Hypersil GOLD Columns....................................................70 BioBasic Columns..............................................................88 Hypercarb Columns .........................................................101 Hypersil BDS Columns ....................................................106 Hypersil Classical Columns.............................................111 Polymeric HPLC Columns ................................................122 AQUASIL C18 Columns ...................................................128 BetaBasic Columns .........................................................129 BetaMax Columns...........................................................130 BETASIL Columns ............................................................130 Other HPLC Columns.......................................................132

65

Accessories for HPLC and GC

HPLC Accessories............................................................136 HPLC Injectors and Valves ..............................................144 GC Accessories ...............................................................150 Syringes...........................................................................161 Vials, Caps and Vial Accessories....................................169

135

Chromatography and Mass Spectrometry Instrument Parts and Supplies

GC and GC/MS................................................................176 Turboow Columns..........................................................188 LC and LC/MS .................................................................189 MS ...............................................................................195 IRMS ...............................................................................198 ICP-MS ............................................................................199

175

Technical Information
SPE Method Development and Troubleshooting............204 HPLC Method Selection, Troubleshooting and Reference Data...............................................207 GC Method Selection, Troubleshooting and Reference Data...............................................221

203

Applications
Pharma/Biochem.............................................................230 Environmental .................................................................258 Forensics/Toxicology .......................................................276 Industrial .........................................................................288 Food Safety .....................................................................302

229

Indexes
Applications Index...........................................................318 Alphabetical Index ..........................................................330

317

Trademark Information

Trademark Information
The Thermo Scientic name, the Thermo Scientic logo and the following trademarks are the property of Thermo Fisher Scientic Inc. and/or its subsidiaries:
BetaBasic BetaMax BETASIL BioBasic BioMate BioWorks ChromQuest COOL POCKET DASH Deca DELTABOND Discovery DSQ Dual Desolvation Zone Evolution Fingerlok Finnigan Fluophase FocusLiner FOCUS HOT POCKET Hypercarb HyperGEL Hypersil Hypersil GOLD Hypersil GOLD aQ HyperPrep HyperQuad HyperREZ HyperSep HyPURITY HyPURITY ADVANCE HyPURITY AQUASTAR Ion Bright Javelin KAPPA LCQ LCQ Advantage LightPipe LTQ LTQ FT LXQ MSQ Open Access PRISM ProteomeX Retain SLIPFREE Smart Accessories SpectraSYSTEM Spectronic Surveyor Surveyor MSQ Plus Surveyor Plus TRACE TRACE GC Ultra Transcend TriPlus TSQ Quantum TSQ Quantum Ultra UNIGUARD Verify Watson LIMS Xcalibur

The following brands, trademarks or service marks are the property of the listed company and/or its subsidiaries. Every effort has been taken to ensure this list is accurate at the time of printing this catalog.
3M Company Kel-F Agilent Technologies, Inc. AccuBOND, Agilent, DB, Evidex, HP-INNOWax, HP-Ultra, Zorbax Applera Corporation POROS Becton, Dickinson and Company Luer-LOK Bio-Rad Laboratories, Inc. Aminex Biotage AB EVOLUTE, Isolute Bristol-Myers Squibb Company Taxol Capital Analytical, Ltd. RH Chromatography Research Supplies, Inc. BTO Cohesive Technologies, Inc. Aria Dow Chemical Company, The TRITON X-100 E.I. Dupont de Nemours and Company Teon, Tefzel, Vespel, Viton Eka Chemicals AB Kromasil Floridin Company Florisil General Electric Company SE GL Sciences, Inc. Inertsil Hamilton Company Hamilton HighChem, Ltd. Mass Frontier Hewlett-Packard Development Company HP Kimberly-Clark Corporation Kimwipes Macherey-Nagel GmbH CHROMABOND, NUCLEODUR, Nucleosil, Optima Mallinckrodt Baker, Inc. BAKERBOND spe, BAKERBOND, Polar Plus Merck KGaA LiChrosorb, LiChrospher, Purospher Merlin Instrument Company Microseal Neos Corporation Fluox New Objective, Inc. IntegraFrit, PicoFrit Ohio Valley Specialty Chemical Company OV Parker Hannin Corporation Parker PerkinElmer, Inc. Clarus , PE Pzer, Inc. Dostinex Phenomenex, Inc. AQUA, Columbus, Gemini, Jupiter, Luna, Prodigy, strata, Synergi, Ultracarb, Zebron ZB Porvair plc Porvair Quadrex Corporation 007 Restek Corporation Rtx, Stabilwax, Pinnacle, Viva Rheodyne LLC Make-Before-Break (MBB), RheBuild, RheFlex, Rheodyne Scientic Glass Technology Exploitatie B.V. CLICK-ON, Shortix, SGT, Super Clean SGE, Inc. Auto-Sep T, Auto-Sep, BP, BPX, Enduro, SGE, PEEKsil, SilTite, Terry Tool Shimadzu Corporation Shimadzu Showa Denko K.K. Asahipak, Shodex Sigma-Aldrich Corporation Discovery, Meridian MDN, Nukol, Petrocol, PTE, SP, SPB, SPME, Supelclean, Supelcosil, SUPELCOWAX, VOCOL Tosoh Corporation TSKgel Union Carbide Corporation Carbowax United Chemical Technologies, Inc. Clean-Up University of Washington SEQUEST, TurboSEQUEST Upchurch Scientic, Inc. Bottom-of-the-Bottle, Upchurch Scientic Varian, Inc. Bond Elut, Bond Elut Certify, CB, CC, CP-Select, CP-Sil, CP-Wax, Polaris, Pursuit, Varian Velcro Industries B.V. Velcro VICI, Valco Instruments Co., Inc. Cheminert, Valco Victrex plc. VICTREX, PEEK Waters Corporation Bondapak, ACQUITY UPLC, Atlantis, Nova-Pak, Sep-Pak, Styragel, SunFire, Symmetry, Ultrahydrogel, Ultrastyragel, Waters, Bondapak, Waters Spherisorb, Symmetry, SymmetryShield, XBridge, Xterra Whatman, Inc. Partisil W.R. Grace & Company Alltima, AT, Denali, Genesis, Vydac YMC Co., Ltd. ODS-AQ, ODS-A, Pro-C18, YMCbasic, YMC-Pack, YMC

Other Notes:
AquaSil Siliconizing Fluid for treating glass surfaces is sold by Pierce Chemical Co., Rockford, IL

ii

To order or for customer support, please see back cover.

Column Selection

Column Selection
Choosing the appropriate SPE product for your sample preparation or the best column for your HPLC or GC analysis can be a daunting task. This section offers many tools, from USP or EPA listings, to phase characteristics, to ow diagrams to help guide you through the choices so you can make an educated and informed decision. In addition to the column selection material presented here, we also have experienced technical support personnel available to answer all your questions.

SPE Column Selection

SPE Column Selection

Thermo Scientic HyperSep SPE products have been developed to meet the requirements of todays sample preparation challenges. HyperSep SPE columns and multi-well plates are offered in a range of phases, ideal for use in application areas such as: Pharmaceutical, Biochemical, Environmental, and Food and Beverage. We are committed to ensuring that high quality products are selected for your application. Please refer to the selection guide below to determine the correct product(s) for your particular application.

SPE Phase Selection

To order or for customer support, please see back cover.

Column Selection

SPE Phase Selection by Manufacturer

Thermo Scientic HyperSep Product Alternative To HyperSep C18, page 34 CLEAN-UP C18-U Supelclean ENVI-18 / LC-18 AccuBONDII C18 BAKERBOND spe Polar Plus Isolute C18 CHROMABOND C18 Bond Elut C18 strata C18-U Sep-Pak C18 HyperSep C8, page 34 CLEAN-UP C8 Supelclean ENVI-8 / LC-8 AccuBONDII C8 BAKERBOND spe Octyl C8 Isolute C8 CHROMABOND C8 Bond Elut C8 strata C8 Sep Pak C8 HyperSep Phenyl, page 35 CLEAN-UP Phenyl Supelclean LC-Ph AccuBONDII Phenyl BAKERBOND spe Phenyl Isolute Ph Bond Elut Ph strata Phenyl (PH) HyperSep Silica, page 35 CLEAN-UP Silica Supelclean LC-Si AccuBONDII Silica BAKERBOND spe Silica Gel Isolute Silica CHROMABOND SiOH Bond Elut Si strata Si-1 Sep Pak Si HyperSep Aminopropyl, page 38 CLEAN-UP Aminopropyl Supelclean LC-NH2 AccuBONDII Amino BAKERBOND spe Amino Isolute NH2 CHROMABOND NH2 Bond Elut NH2 strata NH2 Sep-Pak NH2 HyperSep Retain-CX, page 33 HyperSep Retain PEP, page 32

Thermo Scientic HyperSep Product Alternative To HyperSep Florisil, page 38 CLEAN-UP Florisil Supelclean ENVI-Florisil / LC-Florisil AccuBONDII Florisil BAKERBOND spe Florisil Isolute Florisil CHROMABOND Florisil Bond Elut Florisil strata FL-PR Sep Pak Florisil HyperSep SAX, page 36 CLEAN-UP QAX Supelclean LC-SAX AccuBONDII SAX BAKERBOND spe Quaternary amine Isolute SAX CHROMABOND SB Bond Elut SAX strata SAX HyperSep SCX, page 36 CLEAN-UP BCX Supelclean LC-SCX AccuBONDII SCX BAKERBOND spe Aromatic Sulfonic Acid Isolute SCX CHROMABOND SA Bond Elut SCX strata SCX HyperSep Verify-AX, page 37 CLEAN-UP THC Isolute HAX Bond Elut Certify II strata Screen-A HyperSep Verify-CX, page 37 CLEAN-UP DAU Discovery DSC-MCAX AccuBONDII Evidex Isolute HCX CHROMABOND Drug Bond Elut Certify I strata Screen-C Oasis HLB (Waters) Strata-X (Phenomenex) FOCUS (Varian) Easy (Macherey Nagel) Isolute ENV+ (Biotage) StyreScreen DVB (UCT) H2O-philic DVB (JT Baker) Oasis MCX (Waters) Strata-X-C (Phenomenex) StyreScreen DBX (UCT) HyperSep Retain-AX, page 33 Oasis MAX (Waters) StyreScreen QAX (UCT)

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HPLC and LC/MS

HPLC Column Selection

The AUG will show you Hydrophobicity which gives the relative retention on the column. Generally, the higher the hydrophobicity, the greater the retention of neutral compounds and the higher the organic content in the mobile phase. A lower value indicates a need for higher aqueous mobile phases to achieve comparable retention and resolution. The recommended pH Range for the column is illustrated, outside of which column lifetimes will diminish. The Pore Size is shown, with larger pore size columns being more applicable to larger analytes such as proteins or peptides. The % Carbon is related to the hydrophobicity. Below the icon, you will see the particle sizes available, as well as the USP code. These graphics are designed to allow you to quickly compare the main characteristics of multiple stationary phases, allowing you to choose quickly the most appropriate stationary phase for your analysis. For additional help in column selection, please see the back cover to contact our expert Technical Support and tap our expertise to help make the best choice for your application.

Information in the following section will help you make an informed decision on the appropriate HPLC column for your application, based on stationary phase use, analyte properties, LC/MS requirements or USP specications. You will also nd a useful table of Thermo Scientic phases with specications, as well as recommended Thermo Scientic alternatives for other popular columns. Refer to the Advanced User Graphic (AUG) on the corresponding product page (illustrated to the right) for more help and information on column selection.

HPLC Phases and Their Uses

Common Name Alternative Name Functional Group Normal Phase Reverse Phase Ion Exchange HILIC Application

Silica C1 C4 C8 C18 Cyano

Silica SAS Butyl MOS ODS CPS, CN

-OH -(CH3)3 -C4H9 -C8H17 -C18H37 -(CH2)3CN

Non-polar and moderately polar organic compounds. Least retentive of all alkyl group bonded phases for non-polar solutes. Typically used for moderately polar and multi-functional compounds. Shorter retention than C8, C18. Separation of peptides and proteins. Less retentive than C18; normally used for small peptides and proteins, pharmaceuticals, steroids, environmental samples. Most retentive of the alkyl-bonded phases. Used widely for pharmaceuticals, steroids, fatty acids, phthalates, environmental etc. Unique selectivity for polar compounds, more suitable than base silica for normal phase gradient separations. When used in reversed phase, the selectivity is different to that of the C8 and C18 phases. Useful for a wide range of pharmaceutical applications and for mixtures of very different solutes.

Amino

APS

-(CH2)3NH2

HILIC: Carbohydrate analysis and other polar compounds. Weak anion exchanger, anions and organic acids. Normal Phase: Alternative selectivity to silica. Good for aromatics. Aromatic compounds and moderately polar compounds. Extra selectivity and retention for halogenated, polar compounds and isomers.

Phenyl Pentauorophenyl Diol SCX SAX AX Porous graphitic carbon Strong Cation Exchanger Strong Anion Exchanger Anion Exchanger Polyethleneimine (PEI) PGC PFP

-(CH3)C6H5 -C6Fl5 -(CH2)2O CH2(CH2OH)2 -RSO3H-RN+(CH3)3 -(CH2CH2NH-)n 100% carbon

Reversed Phase: Proteins, peptides. Normal Phase: similar selectivity to silica, but less polar. Organic bases. Organic acids, nucleotides and nucleosides. Organic acids, nucleotides and oligonucleotides. Particularly useful for the separation of highly polar compounds that are difcult to retain using conventional silica based columns; separation of structurally similar compounds (e.g., isomers, diastereoisomers).

To order or for customer support, please see back cover.

Column Selection

HPLC Column Selection

Before beginning a new analysis, consider the physical and chemical properties of the analyte(s), the mode of analysis, and how the analyte(s) will interact with the surface of the chromatographic phase. To aid column selection, the following guide may be useful.

HPLC Column Selection for Biomolecules

Considerations for column selection for biomolecules are a little more complex, due to the size and complexity of many biological analytes. The following can be used for guidance in column selection.

For more information on the HPLC analysis of biomolecules, please request our Technical Guide TG20026.

www.thermo.com /columns

HPLC and LC/MS

Column Selection for LC/MS

The Thermo Scientic range offers a broad array of column designs and stationary phases optimized for LC/MS applications. Use the following diagram to help you choose your column design, dimensions and stationary phase to best meet your application needs. A variety of HPLC column hardware congurations are available, designed to give superior results for high speed, high sensitivity, high efciency and convenience. A wide range of stationary phases allows choices for optimized selectivity.

Column Hardware Selection for LC/MS

LC/MS Application Column Hardware Design Description

High throughput analysis

DASH HTS columns

Short, fast columns 20 x 2.1 mm Labeled and serialized Economical multi-packs Direct-connection columns Slim design, 20 mm length 1 mm to 4.6 mm ID Capillary columns 75 m to 500 m ID 30 mm to 250 mm lengths Capillary columns 75 m to 500 m ID Nanobore columns 75 m ID Direct nanospray from column tapered-tip

Javelin HTS columns

High sensitivity analysis

KAPPA capillary columns

Proteomics analysis

KAPPA capillary columns PicoFrit and IntegraFrit nanobore columns

To order or for customer support, please see back cover.

Column Selection

Stationary Phase Selection for LC/MS

Phase Particle Sizes Pore Sizes Stationary Phase Chemistries General Description Page

Hypersil GOLD

1.9 m, 3 m, 5 m

175

C18 selectivity

Outstanding peak shape using generic gradients with C18 selectivity, providing increased peak capacity, improved resolution, sensitivity and signal to noise. Offers similar selectivity to Hypersil GOLD but with less retention. Excellent for polar compounds. Good results with low buffer concentrations. Can be used for challenging reverse phase separations employing highly aqueous mobile phases. Offers alternative selectivity in reverse phase applications, particularly for halogenated analytes. Offers alternative selectivity. Can be used for both reversed and normal phase separations. Offers unique selectivity for the analysis of aromatic and moderately polar compounds. Based on 300 pore size silica specically designed for the separation of protein and peptides with increasing hydrophobicity: cyano and phenyl phases to provide alternative selectivity where required. Large pore size for biomolecules. BioBasic SCX and AX stationary phases comprise silica particles coated with polymeric ion exchange ligands, which shield proteins from adsorbing to the silica surface. BioBasic SEC columns are available with 60 , 120 , 300 and 1000 pore sizes, allowing separation of a wide range of molecular weights. Unique 100% porous graphitic carbon. Exceptional retention of very polar analytes. Separation of structurally similar substances. pH stable from 0 - 14. Ideal for high temperature applications. General purpose packing. Superb high pH stability. General purpose packing with high surface coverage for strong retention and use with high organic mobile phases.

72

Hypersil GOLD C8 Hypersil GOLD aQ

1.9 m, 3 m, 5 m 1.9 m, 3 m, 5 m

175 175

C8 C18 polar endcapped

76 78

Hypersil GOLD PFP Hypersil GOLD CN

1.9 m, 3 m, 5 m 1.9 m, 3 m, 5 m

175 175 175 300

Peruorinated phenyl Cyano Phenyl C18, C8, C4, CN, Phenyl

81 84 86

Hypersil GOLD Phenyl 1.9 m, 3 m, 5 m BioBasic Reversed Phase 5 m

91

BioBasic Ion Exchange Phase

5 m

300

SCX, AX

97

BioBasic SEC

5 m

60 , 120 , 300 , 1000 250

SEC

89

Hypercarb

3 m, 5 m

Porous Graphitic Carbon

101

BetaBasic BETASIL

3 m, 5 m 3 m, 5 m 5 m

150 100

C18, C8, C4, CN, Phenyl C18, C8, C6, Phenyl/Hexyl, Cyano, Silica C1, Diol

129 130

www.thermo.com /columns

HPLC and LC/MS

HPLC Column Selection by U.S. Pharmacopeia Specications*

USP Code Description Recommended Phase Page

L1

L2 L3 L4 L7

L8 L9 L10

L11

L13 L14 L15 L17 L19 L20 L21 L22 L25 L26 L27 L33

L34 L43 L52 L58 L59 L60

Hypersil GOLD Hypersil GOLD aQ Hypersil GOLD 1.9 m Hypersil GOLD aQ 1.9 m BioBasic 18 Hypersil BDS C18 Hypersil ODS Octadecyl silane chemically bonded to a silica gel of controlled surface porosity bonded to a solid spherical core, 30 -50 m in diameter Pellicular ODS Porous silica microparticles, 5 -10 m in diameter BETASIL Silica Hypersil Silica Silica gel of controlled surface porosity bonded to a solid spherical core, 30 - 50 m in diameter Pellicular Silica Octyl silane (C8) chemically bonded to totally porous silica particles, 1.5 -10 m in diameter Hypersil GOLD C8 Hypersil GOLD C8 1.9 m BioBasic 8 Hypersil BDS C8 Hypersil MOS Hypersil MOS-2 An essentially monomolecular layer of aminopropylsilane chemically bonded to totally porous silica gel support, 3 - 10 m in diameter Hypersil APS-2 Irregular or spherical, totally porous silica gel having a chemically bonded, strongly acidic cation Partisil SCX exchange coating (SCX), 3 - 10 m in diameter Nitrile groups (CN) chemically bonded to porous silica particles, 3 -10 m in diameter Hypersil GOLD CN BioBasic CN Hypersil BDS Cyano Hypersil CPS Hypersil CPS-2 Phenyl groups chemically bonded to porous silica particles, 1.5 - 10 m in diameter Hypersil GOLD Phenyl Hypersil GOLD Phenyl 1.9 m BioBasic Phenyl Hypersil BDS Phenyl Hypersil Phenyl Hypersil Phenyl-2 Trimethylsilane chemically bonded to porous silica particles, 3 - 10 m in diameter Hypersil SAS BETASIL C1 Silica gel having a chemically bonded, strongly basic quaternary ammonium anion exchange (SAX) coating, 5 - 10 m in diameter Partisil SAX Hypersil SAX (5 m) Hexylsilane (C6) chemically bonded to totally porous silica particles, 3 -10 m in diameter BETASIL C6 Strong cation exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the hydrogen form, HyperREZ XP Carbohydrate H+ 7 -11 m in diameter HyperREZ XP Organic Acids Strong cation exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the calcium form, HyperREZ XP Carbohydrate Ca2+ about 9 m diameter HyperREZ XP Sugar Alcohols Dihydroxypropane groups chemically bonded to porous silica particles, 5 - 10 m in diameter BETASIL Diol A rigid spherical styrene-divinylbenzene copolymer, 5 - 10 m in diameter HyperREZ XP RP100 HyperREZ XP RP300 A cation exchange resin made of porous polystyrene gel with sulfonic acid groups, about 10 m in size HyperREZ XP SCX Packing having the capacity to separate compounds with a molecular weight range 100 - 5000 (as determined by HyperGEL AP polyethylene oxide) applied to neutral, anionic and cationic water-soluble polymers. Butyl silane (C4) chemically bonded to totally porous silica particles, 3 -10 m in diameter BioBasic 4 BetaBasic 4 Porous silica particles, 30 - 50 m in diameter HyperPrep Silica Packing having the capacity to separate dextrans by molecular size over a range of 4,000 to 500,000 daltons. BioBasic SEC 120 It is spherical, silica-based, and processed to provide pH stability BioBasic SEC 300 BioBasic SEC 1000 Strong cation exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the lead form, HyperREZ XP Carbohydrate Pb2+ about 9 m in diameter Pentauorophenyl groups chemically bonded to silica particles by a propyl spacer, 5 -10 m in diameter Hypersil GOLD PFP Fluophase PFP A strong cation exchange resin made of porous silica with sulfopropyl groups by a propyl spacer, 5 - 10 m in diameter BioBasic SCX Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the sodium form, HyperREZ XP Carbohydrate Na+ about 7 to 11 m in diameter Packing having the capacity to separate proteins by molecular weight over the range of 10 to 500 kDa. BioBasic SEC 300 (5 m) It is spherical (10 m), silica-based, and processed to provide hydrophilic characteristics and pH stability Spherical, porous silica gel, 10 m or less in diameter, the surface of which has been covalently modied with HyPURITY ADVANCE alkyl amide groups and endcapped

Octadecyl silane (C18) chemically bonded to porous or ceramic microparticles, 1.5 -10 m in diameter, or a monolithic rod

72 78 72 78 91 107 112 inquire 130 119 inquire 76 76 93 108 114 114 118 inquire 84 96 110 117 117 86 86 95 109 116 116 115 130 inquire 120 130 123 123 123 123 130 inquire inquire inquire 125 94 129 inquire 89 89 89 123 81 132 98 123 89 133

* These are the recommended Thermo Scientic HPLC columns for various USP categories although other columns for each category are also available.

To order or for customer support, please see back cover.

Column Selection

Thermo Scientic HPLC Phases

The tables below list Thermo Scientic HPLC sorbents offered. Please also refer to the Advanced User Graphic (AUG) for each HPLC phase on the pages indicated.
Phase Particle Type Particle Size (m) Pore Size () Nominal Surface Area (m2/g) % Carbon Endcapping USP Code Phase Code Page

AQUASIL C18 BetaBasic 18 8 4 Phenyl CN BetaMax Neutral Acid Base BETASIL C18 C8 C6 C1 Phenyl Phenyl-Hexyl CN Silica 100 Diol 100 BioBasic 18 8 4 Phenyl CN AX SCX DELTABOND Resolution AK spherical, polymer coated silica Fast AK Fluophase RP PFP WP Hypercarb Hypercarb HyperREZ XP Carbohydrate H+ Carbohydrate Pb2+ Carbohydrate Ca2+ Carbohydrate Na+ Organic Acid Sugar Alcohol spherical, polymer spherical, polymer spherical, polymer spherical, polymer spherical, polymer spherical, polymer 8 8 8 10 8 8 L17 L34 L19 L17 L19 690 691 692 693 696 697 123 123 123 123 123 123 spherical, porous graphitic carbon 3, 5, 7 250 120 100 350 101 spherical, silica spherical, silica spherical, silica 5 5 5 100 100 300 310 310 100 10 12 5 Yes Yes Yes L43 825 827 826 132 132 132 spherical, polymer coated silica 5 5 300 300 100 100 12 12 No No 322 323 132 132 spherical, silica spherical, silica spherical, silica spherical, silica spherical, silica spherical, silica spherical, silica 5 5 5 5 5 5 5 300 300 300 300 300 300 300 100 100 100 100 100 100 100 9 5 4 3 3.5 3 3 Yes Yes Yes Yes Yes No L1 L7 L26 L11 L10 L52 721 722 723 724 729 731 732 91 93 94 95 96 97 98

spherical, silica

3, 5

100

310

12

polar

L1

775

128

spherical, silica spherical, silica spherical, silica spherical, silica spherical, silica

3, 5 3, 5 3, 5 3, 5 3, 5

150 150 150 150 150

200 200 200 200 200

13 7 6 7 5

Yes Yes Yes Yes Yes

L1 L7 L26 L11 L10

715 714 716 718 717

129 129 129 129 129

spherical, silica spherical, silica spherical, silica

5 5 5

60 60 60

540 540 540

29 15 9

Yes Yes Yes

L1 L28 L10

950 952 951

130 130 130

spherical, silica spherical, silica spherical, silica spherical, silica spherical, silica spherical, silica spherical, silica spherical, silica spherical, silica

3, 5,10 3, 5, 10 3, 5 5 3, 5 3, 5 3, 5 3, 5 5

100 100 100 100 100 100 100 100 100

310 310 310 310 310 310 310 310 310

20 12 11 4 11 11 6 6

Yes Yes Yes Yes Yes Yes Yes

L1 L7 L15 L13 L11 L11 L10 L3 L20

701 702 703 705 706 730 708 700 726

130 130 130 130 130 130 130 130 130

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HPLC and LC/MS

Thermo Scientic HPLC Phases

Phase Particle Type Particle Size (m) Pore Size () Nominal Surface Area (m2/g) % Carbon Endcapping USP Code Phase Code Page

Hypersil ODS (C18) ODS-2 (C18) MOS (C8) MOS-2 (C8) Butyl (C4) SAS (C1) Phenyl Phenyl-2 CPS CPS-2 APS-2 Silica SAX Hypersil BDS C18 C8 Phenyl Cyano Hypersil GOLD C18 selectivity C8 CN (Cyano) PFP aQ Phenyl Hypersil Green PAH HyPURITY C18 C8 C4 Cyano ADVANCE AQUASTAR

spherical, silica spherical, silica spherical, silica spherical, silica spherical, silica spherical, silica spherical, silica spherical, silica spherical, silica spherical, silica spherical, silica spherical, silica spherical, silica

3, 5, 10 3, 5 3, 5, 10 3, 5, 10 3, 5, 10 3, 5, 10 3, 5, 10 5, 10 3, 5, 10 5, 10 3, 5, 10 3, 5, 10 5

120 80 120 120 120 120 120 120 120 120 120 120 120

170 220 170 170 170 170 170 170 170 170 170 170 170

10 11 6.5 6.5 2.5 5 5 4 4 1.9 2.5

Yes Yes No Yes Yes No Yes No Yes No Yes

L1 L1 L7 L7 L26 L13 L11 L11 L10 L10 L8 L3 L14

301 316 302 303 304 305 309 319 308 318 307 300 341

112 113 114 114 inquire 115 116 116 117 117 118 119 120

spherical, silica spherical, silica spherical, silica spherical, silica

3, 5 3, 5 3, 5 3, 5

130 130 130 130

170 170 170 170

11 7 5 4

Yes Yes Yes Yes

L1 L7 L11 L10

281 282 289 288

107 108 109 110

spherical, silica spherical, silica spherical, silica spherical, silica spherical, silica spherical, silica

1.9, 3, 5, 8, 12 1.9, 3, 5 1.9, 3, 5 1.9, 3, 5, 8, 12 1.9, 3, 5, 8, 12 1.9, 3, 5

175 175 175 175 175 175

220 220 220 220 220 220

10 8 4 8 12 8.5

Yes Yes Yes Yes Polar Yes

L1 L7 L10 L43 L1 L11

250 252 258 254 253 259

72 76 84 81 78 86

spherical, silica

3, 5

120

170

13.5

Yes

311

121

spherical, silica spherical, silica spherical, silica spherical, silica spherical, silica spherical, silica

3, 5, 8, 12 5 5 5 3, 5 3, 5, 8, 12

190 190 190 190 190 190

200 200 200 200 200 200

13 8 4.5 4 10 10

Yes Yes Yes Yes Polar

L1 L7 L26 L10 L60 L1

221 222 224 228 210 225

133 133 133 133 133 133

Size Exclusion Chromatography Phases

Phase Type Particle Type Particle Size (m) Pore Size () Exclusion Limit Operating Range USP Code Packing Code Page

HyperGEL OP5 OP10 OP25 OP30 OP40 OP50 OP60 OP BioBasic SEC 60 SEC 120 SEC 300 SEC 1000 aqueous aqueous aqueous aqueous silica silica silica silica 5 5 5 5 60 120 300 1,000 0.1- 6 0.1- 50 1- 500 20 - 4,000 L33 L33, L59 L33 733 734 735 736 89 89 89 89 organic organic organic organic organic organic organic organic PS-DVB polymer PS-DVB polymer PS-DVB polymer PS-DVB polymer PS-DVB polymer PS-DVB polymer PS-DVB polymer PS-DVB polymer 5, 10 5, 10 5, 10 5, 10 5, 10 5, 10 5, 10 5, 10 50 100 500 1,000 10,000 100,000 1,000,000 up to 2,000* up to 4,000* 500 - 30,000* 500 - 60,000* 10,000 - 600,000* 60,000 - 2,000,000* 600,000 - 10,000,000* 430 431 432 433 434 435 436 437 125 125 125 125 125 125 125 125

* Operating MW range PEO/PEG (g/mol)

Separation range, protein (kDa)

10

To order or for customer support, please see back cover.

Column Selection

HPLC Column Selection by Manufacturer

To nd a suitable Thermo Scientic alternative to another manufacturers columns, refer to the selection guide below. The Thermo Scientic alternative phases are selected based on a combination of physical and chemical similarities as well as mode of retention. These alternatives are not guaranteed to provide the same retention or selectivity, but should be suitably similar in character to allow a similar or improved separation to be achieved with some method optimization. The user should refer to the individual phase information to ensure that the characteristics of the alternative match the requirements of their separation. The following table is not complete in terms of manufacturer or products offered. Although every effort is made to ensure that the product information provided is as accurate as possible, some errors may occur in collation and transcription. We can not accept any responsibility for the use of the following information.

Phase

Manufacturer

Pore Size ()

Area (m2/g)

%C

Recommended Thermo Scientic Alternative

Page

ACE C18 ACE C8 ACE C4 ACE CN ACE Phenyl ACE AQ ACE C18-300 ACE C8-300 ACE C4-300 ACE CN-300 ACE Phenyl-300 ACQUITY UPLC BEH C18 ACQUITY UPLC BEH C8 ACQUITY UPLC BEH Phenyl ACQUITY UPLC HSS T3 Alltima HP C18 Alltima HP C18 Amide Alltima HP C18 AQ Alltima HP C18 HiLoad Alltima HP C18-Amide Alltima HP C8 Alltima HP CN Alltima HP Silica Aminex HPX42C Aminex HPX72S Aminex HPX87C Aminex HPX87H Aminex HPX87N Aminex HPX87P AQUA C18 Ascentis C18 Ascentis C8 Ascentis RP-Amide Ascentis Phenyl Atlantis dC18 Columbus C18 Columbus C8

ACT ACT ACT ACT ACT ACT ACT ACT ACT ACT ACT Waters Waters Waters Waters Grace Grace Grace Grace Grace Grace Grace Grace Bio-Rad Bio-Rad Bio-Rad Bio-Rad Bio-Rad Bio-Rad Phenomenex Supelco Supelco Supelco Supelco Waters Phenomenex Phenomenex

100 100 100 100 100 100 300 300 300 300 300 130 130 130 100 190 190 100 100 190 190 190 190 125 100 100 100 100 100 110 110

300 300 300 300 300 300 100 100 100 100 100 185 185 185 230 200 200 450 450 200 200 200 200 320 450 450 450 450 330 375 375

15.5 9 5.5 5.5 9.5 14 9 5 2.6 2.6 5.3 12 12 20 24 12 8 4 15 25 15 19.5 19 12 19 13

Hypersil GOLD Hypersil GOLD C8 HyPURITY C4 Hypersil GOLD CN Hypersil GOLD Phenyl Hypersil GOLD aQ BioBasic 18 BioBasic 8 BioBasic 4 BioBasic CN BioBasic Phenyl Hypersil GOLD (1.9 m) Hypersil GOLD C8 (1.9 m) Hypersil GOLD Phenyl (1.9 m) Hypersil GOLD aQ (1.9 m) Hypersil GOLD HyPURITY ADVANCE Hypersil GOLD aQ BETASIL C18 HyPURITY ADVANCE Hypersil GOLD C8 Hypersil GOLD CN BETASIL Silica HyperREZ XP Carbohydrate Ca2+ HyperREZ XP Carbohydrate H+ HyperREZ XP Carbohydrate Ca2+ HyperREZ XP Carbohydrate H+ HyperREZ XP Carbohydrate Na+ HyperREZ XP Carbohydrate Pb2+ Hypersil GOLD aQ Hypersil GOLD BETASIL C18 Hypersil GOLD C8 BETASIL C8 HyPURITY ADVANCE BetaMax Acid Hypersil GOLD Phenyl BETASIL Phenyl Hypersil GOLD aQ Hypersil GOLD BETASIL C18 Hypersil GOLD C8

72 76 133 84 86 78 91 93 94 96 95 72 76 86 78 72 133 78 130 133 76 84 130 123 123 123 123 123 123 78 72 130 76 130 133 130 86 130 78 72 130 76

www.thermo.com /columns

11

HPLC and LC/MS

HPLC Column Selection by Manufacturer

Phase Manufacturer Pore Size () Area (m2/g) %C Recommended Thermo Scientic Alternative Page

DENALI C18 Discovery Amide C16 Discovery BIO Wide Pore C18 Discovery BIO Wide Pore C8 Discovery C18 Discovery C8 Discovery Cyano Fluox 120 Gemini C18 Genesis AQ Genesis C4 Genesis C8 Genesis CN Genesis MOS Genesis ODS Genesis Phenyl Genesis Silica Inertsil C4 Inertsil C8 Inertsil ODS3V Inertsil Phenyl Inertsil Silica Jupiter C18 Jupiter C4 Kromasil C1 Kromasil C18 Kromasil C4 Kromasil Silica LiChrospher CN LiChrospher Diol LiChrospher NH2 LiChrospher RP 18 LiChrospher RP-18e LiChrospher RP-8 LiChrospher RP-8e Luna C18 (2) Luna C8 (2) Luna CN Luna NH2 Luna SCX Luna Silica (2) Bondapak C18 Bondapak CN Bondapak NH2 Bondapak Phenyl Nova-Pak (HR) C18 Nova-Pak C8 Nova-Pak CN Nova-Pak Phenyl Nova-Pak Silica NUCLEODUR C18 EC NUCLEODUR C18 Gravity NUCLEODUR CN

Grace Supelco Supelco Supelco Supelco Supelco Supelco Neos Phenomenex Grace Grace Grace Grace Grace Grace Grace Grace GL Sciences GL Sciences GL Sciences GL Sciences GL Sciences Phenomenex Phenomenex Akzo-Nobel Akzo-Nobel Akzo-Nobel Akzo-Nobel Merck Merck Merck Merck Merck Merck Merck Phenomenex Phenomenex Phenomenex Phenomenex Phenomenex Phenomenex Waters Waters Waters Waters Waters Waters Waters Waters Waters Macherey-Nagel Macherey-Nagel Macherey-Nagel

120 180 300 300 180 180 180 120 110 120 120 120 120 120 120 120 120 150 150 100 150 150 300 300 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 125 125 125 125 60 60 60 60 60 110 110 110

200 200 200 200 375 300 300 300 300 300 300 300 300 320 320 450 320 320 170 170 340 340 340 340 350 350 350 350 350 350 350 400 400 400 400 400 400 330 330 330 330 120 120 120 120 120 340 340 340

11 14 5 14 7 11 18 8 11 15 10 13 5 5 19 8 7 5 21 22 13 13 18 14 10 10 4 7 5 18 18 7

Hypersil GOLD HyPURITY ADVANCE BioBasic 18 BioBasic 8 Hypersil GOLD Hypersil GOLD C8 Hypersil GOLD CN Fluophase RP Hypersil GOLD Hypersil GOLD aQ HyPURITY C4 Hypersil GOLD C8 Hypersil GOLD CN Hypersil GOLD C8 Hypersil GOLD Hypersil GOLD Phenyl BETASIL Silica HyPURITY C4 Hypersil GOLD C8 Hypersil GOLD Hypersil GOLD Phenyl BETASIL Silica BioBasic 18 HyPURITY C4 BETASIL C1 Hypersil GOLD BETASIL C18 HyPURITY C4 BETASIL Silica Hypersil GOLD CN BETASIL Diol Hypersil APS-2 Hypersil GOLD BETASIL C18 Hypersil GOLD BETASIL C18 Hypersil GOLD C8 Hypersil GOLD C8 Hypersil GOLD Hypersil GOLD C8 Hypersil GOLD CN Hypersil APS-2 BioBasic SCX BETASIL Silica Hypersil GOLD Hypersil GOLD CN Hypersil APS-2 Hypersil GOLD Phenyl Hypersil GOLD Hypersil GOLD C8 Hypersil GOLD CN Hypersil GOLD Phenyl BETASIL Silica Hypersil GOLD Hypersil GOLD Hypersil GOLD CN

72 133 91 93 72 76 84 132 76 78 133 76 84 76 72 86 130 133 76 72 86 130 91 133 130 72 130 133 130 84 130 118 72 130 72 130 76 76 72 76 84 118 98 130 72 84 118 86 72 76 84 86 130 72 72 84

12

To order or for customer support, please see back cover.

Column Selection

Phase

Manufacturer

Pore Size ()

Area (m2/g)

%C

Recommended Thermo Scientic Alternative

Page

NUCLEODUR Pyramid Nucleosil 100 C18 Nucleosil 100 C18 AB Nucleosil 100 C6H5 Nucleosil 100 C8 Nucleosil 100 CN Nucleosil 100 N(CH3)2 Nucleosil 100 NH2 Nucleosil 100 OH Nucleosil 100 SA Nucleosil 100 SB Nucleosil 300 C18 Nucleosil 300 C4 Nucleosil 300 C6H5 Nucleosil 300 C8 Nucleosil 300 CN Partisil C8 Partisil ODS Partisil ODS2 Partisil ODS-3 Partisil SAX Partisil SCX Partisil Silica Pinnacle C1 Pinnacle C18 Pinnacle C4 Pinnacle CN Pinnacle DB C18 Pinnacle DB C18 1.9 m Pinnacle DB C8 Pinnacle DB Cyano Pinnacle DB Phenyl Pinnacle IBD Pinnacle NH2 Pinnacle Phenyl Pinnacle SAX Pinnacle Silica Pinnacle Ultra C18 Pinnacle Wide Pore C4 Polaris C18 Ether Polaris C18-A Polaris C8 Ether Polaris C8-A Polaris NH2 Prodigy C8 Prodigy ODS2 Prodigy ODS-3 Prodigy ODS-3V Prodigy Phenyl-3 Purospher RP-18 Purospher STAR-8e Purospher STAR RP-18e

Macherey-Nagel Macherey-Nagel Macherey-Nagel Macherey-Nagel Macherey-Nagel Macherey-Nagel Macherey-Nagel Macherey-Nagel Macherey-Nagel Macherey-Nagel Macherey-Nagel Macherey-Nagel Macherey-Nagel Macherey-Nagel Macherey-Nagel Macherey-Nagel Whatman Whatman Whatman Whatman Whatman Whatman Whatman Restek Restek Restek Restek Restek Restek Restek Restek Restek Restek Restek Restek Restek Restek Restek Restek Varian Varian Varian Varian Varian Phenomenex Phenomenex Phenomenex Phenomenex Phenomenex Merck Merck Merck

110 100 100 100 100 100 100 100 100 100 100 300 300 300 300 300 85 85 85 85 85 85 85 120 120 120 120 140 140 140 140 140 120 120 120 120 120 100 300 150 150 100 100 100 60 120 120

340 350 350 350 350 350 350 350 350 350 350 100 100 100 100 100 350 350 350 350 350 350 350 170 170 170 170 170 170 170 170 170 310 310 450 450 450 500 300 300

14 17 24 9 4 7 10 7 9 5 16 11 2 10 4 5 11 11 6 4 5 2 5 3 20 2 13 18 16 16 10

Hypersil GOLD aQ Hypersil GOLD Hypersil GOLD BETASIL C18 Hypersil GOLD Phenyl Hypersil GOLD C8 Hypersil GOLD CN Hypersil SAX Hypersil APS-2 BETASIL Diol BioBasic SCX Hypersil SAX BioBasic 18 BioBasic 4 BioBasic Phenyl BioBasic 8 BioBasic CN Hypersil GOLD C8 Hypersil GOLD Hypersil GOLD Hypersil GOLD Hypersil SAX BioBasic SCX BETASIL Silica Hypersil SAS Hypersil GOLD HyPURITY C4 Hypersil GOLD CN Hypersil GOLD Hypersil GOLD (1.9 m) Hypersil GOLD C8 Hypersil GOLD CN Hypersil GOLD Phenyl Hypersil GOLD Hypersil APS-2 Hypersil GOLD Phenyl Hypersil SAX BETASIL Silica Hypersil GOLD BETASIL C18 BioBasic 4 HyPURITY ADVANCE HyPURITY ADVANCE HyPURITY ADVANCE HyPURITY ADVANCE Hypersil APS-2 Hypersil GOLD C8 Hypersil GOLD BETASIL C18 Hypersil GOLD Hypersil GOLD BETASIL Phenyl Hypersil GOLD Hypersil GOLD C8 Hypersil GOLD

78 72 72 130 86 76 84 120 118 130 98 120 91 94 95 93 96 76 72 72 72 120 98 130 115 72 133 84 72 72 76 84 95 72 118 95 120 130 72 130 94 133 133 133 133 118 76 72 130 72 72 130 72 76 72

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13

HPLC and LC/MS

HPLC Column Selection by Manufacturer

Phase Manufacturer Pore Size () Area (m2/g) %C Recommended Thermo Scientic Alternative Page

Purospher RP-18e Pursuit C18 Pursuit C8 Pursuit Diphenyl Pursuit PFP Shodex OHpak SB802.5 Shodex OHpak SB803 Shodex OHpak SB804 Shodex OHpak SB806 Shodex PH Shodex SIL Shodex TMS Waters Spherisorb C1 Waters Spherisorb C6 Waters Spherisorb C8 Waters Spherisorb CN Waters Spherisorb NH2 Waters Spherisorb ODS1 Waters Spherisorb ODS2 Waters Spherisorb ODSB Waters Spherisorb Phenyl Waters Spherisorb SAX Waters Spherisorb SCX Waters Spherisorb W (silica) Styragel HR0.5 Styragel HR1 Styragel HR2 Styragel HR3 Styragel HR4 Styragel HT3 Styragel HT4 SunFire C18 SunFire C8 Supelcosil ABZ Supelcosil ABZ+ Supelcosil LC-1 Supelcosil LC-18 Supelcosil LC-18DB Supelcosil LC-8 Supelcosil LC-CN Supelcosil LC-NH2 Supelcosil LC-Si Symmetry C18 Symmetry C8 SymmetryShield C18 SymmetryShield C8 Synergi Fusion-RP Synergi Hydro-RP TSKgel G2000SW (incl XL) TSKgel Octyl-80TS TSKgel ODS-120A TSKgel ODS-120A

Merck Varian Varian Varian Varian Showa Denko Showa Denko Showa Denko Showa Denko Showa Denko Showa Denko Showa Denko Waters Waters Waters Waters Waters Waters Waters Waters Waters Waters Waters Waters Waters Waters Waters Waters Waters Waters Waters Waters Waters Supelco Supelco Supelco Supelco Supelco Supelco Supelco Supelco Supelco Waters Waters Waters Waters Phenomenex Phenomenex Tosoh Tosoh Tosoh Tosoh

60 100 100 100 80 80 80 80 80 80 80 80 80 80 80 80 50 100 500 1,000 10,000 1,000 10,000 90 90 120 120 120 120 120 120 120 120 120 100 100 100 100 80 80 125 80 120 120

500 200 200 200 200 200 200 200 200 200 200 200 200 340 340 170 170 170 170 170 170 170 335 335 335 335 475 475 200 200 200

2 5 6 3 2 6 12 12 3 16 16 12 12 11 11 19 12 17 15 19 11 22 22

Hypersil GOLD Hypersil GOLD Hypersil GOLD C8 BetaBasic Phenyl Hypersil GOLD PFP HyperGEL AP HyperGEL AP HyperGEL AP HyperGEL AP Hypersil GOLD Phenyl BETASIL Silica Hypersil SAS Hypersil SAS BETASIL C6 Hypersil GOLD C8 Hypersil GOLD CN Hypersil APS-2 Hypersil GOLD Hypersil GOLD Hypersil GOLD Hypersil GOLD Phenyl Hypersil SAX BioBasic SCX BETASIL Silica HyperGEL OP 5 HyperGEL OP 10 HyperGEL OP 25 HyperGEL OP 30 HyperGEL OP 40 HyperGEL OP 30 HyperGEL OP 40 Hypersil GOLD Hypersil GOLD C8 HyPURITY ADVANCE HyPURITY ADVANCE Hypersil SAS Hypersil GOLD Hypersil GOLD Hypersil GOLD C8 Hypersil GOLD CN Hypersil APS-2 BETASIL Silica Hypersil GOLD Hypersil GOLD C8 HyPURITY ADVANCE HyPURITY ADVANCE HyPURITY ADVANCE Hypersil GOLD aQ BioBasic SEC 120 Hypersil GOLD C8 Hypersil GOLD BETASIL C18

72 72 76 129 81 125 125 125 125 86 130 115 115 130 76 84 118 72 72 72 86 120 98 130 125 125 125 125 125 125 125 72 76 133 133 115 72 72 76 84 118 130 72 76 133 133 133 78 89 76 72 130

14

To order or for customer support, please see back cover.

Column Selection

Phase

Manufacturer

Pore Size ()

Area (m2/g)

%C

Recommended Thermo Scientic Alternative

Page

TSKgel ODS-120T TSKgel ODS-80TM TSKgel Super Octyl TSKgel Super ODS TSKgel Super Phenyl TSKgel SuperSW3000 Ultracarb C8 Ultracarb ODS (20) Ultracarb ODS (30) Ultrahydrogel 1000 Ultrastyragel 100A Ultrastyragel 103A Ultrastyragel 104A Ultrastyragel 105A Ultrastyragel 106A Ultrastyragel 500A Viva C18 Viva C4 Viva C8 Vydac 201SP C18 Vydac 201SP Selectapore 90M C18 Vydac 201TP C18 Vydac 202TP C18 Vydac 208TP C8 Vydac 214TP Vydac 218TP Vydac 218WP Selectapore 300M C18 Vydac 219TP Vydac 238TP Vydac 259VHP Vydac 300VHP Vydac 301VHP Vydac 400VHP XBridge C18 XBridge C8 XBridge Phenyl XBridge Shield RP18 XTerra MS C18 XTerra MS C8 XTerra Phenyl XTerra RP18 XTerra RP8 YMCbasic YMC-Pack C4 YMC-Pack C8 YMC-Pack CN YMC-Pack Diol YMC-Pack NH2 YMC-Pack ODS AQ YMC-Pack ODS-A YMC-Pack ODS-A YMC-Pack Phenyl YMC-Pack Phenyl

Tosoh Tosoh Tosoh Tosoh Tosoh Tosoh Phenomenex Phenomenex Phenomenex Waters Waters Waters Waters Waters Waters Waters Restek Restek Restek Grace Grace Grace Grace Grace Grace Grace Grace Grace Grace Grace Grace Grace Grace Waters Waters Waters Waters Waters Waters Waters Waters Waters YMC YMC YMC YMC YMC YMC YMC YMC YMC YMC YMC

120 80 110 110 110 250 60 90 60 1000 100 1,000 10,000 100,000 1,000,000 500 300 300 300 90 90 300 300 300 300 300 300 300 300 300 300 300 300 125 125 125 125 125 120 120 120 120 120 120 120 300 120 300

200 200 550 320 550 250 70 180 180 180 180 180 300 300 300 300 300 300 150 300 150

22 15 5 8 3 14 22 31 9 4 5 16 12 12 16 14 7 10 7 16 17 6 9 3

Hypersil GOLD BETASIL C18 Hypersil GOLD Hypersil GOLD C8 Hypersil GOLD Hypersil GOLD Phenyl BioBasic SEC 300 Hypersil GOLD C8 Hypersil GOLD BETASIL C18 BetaMax Neutral HyperGEL AP 30 HyperGEL OP 10 HyperGEL OP 30 HyperGEL OP 40 HyperGEL OP 50 HyperGEL OP 60 HyperGEL OP 25 BioBasic 18 BioBasic 4 BioBasic 8 Hypersil GOLD Hypersil GOLD BioBasic 18 BioBasic 18 BioBasic 8 BioBasic 4 BioBasic 18 BioBasic 18 BioBasic Phenyl BioBasic 18 HyperREZ XP RP 300 HyperREZ XP SAX HyperREZ XP SAX HyperREZ XP SCX Hypersil GOLD Hypersil GOLD C8 Hypersil GOLD Phenyl HyPURITY ADVANCE Hypersil GOLD Hypersil GOLD C8 Hypersil GOLD Phenyl HyPURITY ADVANCE HyPURITY ADVANCE Hypersil GOLD C8 HyPURITY C4 Hypersil GOLD C8 Hypersil GOLD CN BETASIL Diol Hypersil APS-2 Hypersil GOLD aQ Hypersil GOLD BioBasic 18 Hypersil GOLD Phenyl BioBasic Phenyl

72 130 72 76 72 86 89 76 72 130 130 125 125 125 125 125 125 125 91 94 93 72 72 91 91 93 94 91 91 95 91 inquire inquire inquire inquire 72 76 86 133 72 76 86 133 133 76 133 76 84 130 118 78 72 91 86 95

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15

HPLC and LC/MS

HPLC Column Selection by Manufacturer

Phase Manufacturer Pore Size () Area (m2/g) %C Recommended Thermo Scientic Alternative Page

YMC-Pack Polyamine 2 YMC-Pack Polymer C18 YMC-Pack Pro C18 YMC-Pack Silica YMC-Pack TMS (C1) Zorbax Bonus RP Amide C14 Zorbax Eclipse XDB C18 Zorbax Eclipse XDB C8 Zorbax Eclipse XDB Phenyl Zorbax Eclipse Plus C18 Zorbax Eclipse Plus C8 Zorbax RRHT Eclipse Plus C18 Zorbax RRHT Eclipse Plus C8 Zorbax RRHT Eclipse XDB-C18 Zorbax RRHT Eclipse XDB-C8 Zorbax RRHT SB-CN Zorbax SB Aq Zorbax SB C18 Zorbax SB C18 Zorbax SB C8 Zorbax SB C8 Zorbax SB CN Zorbax SB CN Zorbax SB Phenyl

YMC YMC YMC YMC YMC Agilent Agilent Agilent Agilent Agilent Agilent Agilent Agilent Agilent Agilent Agilent Agilent Agilent Agilent Agilent Agilent Agilent Agilent Agilent

120 120 120 120 80 80 80 80 95 95 95 95 80 80 80 80 80 300 80 300 80 300 80

350 300 180 180 180 180 160 160 160 160 180 180 180 180 180 45 180 45 180 45 180

16 4 10 10 8 8 8 6 8 6 10 7.5 4 10 3 6 2 4 1 6

HyperREZ SAX Hypersil GOLD Hypersil GOLD BETASIL Silica BETASIL C1 HyPURITY ADVANCE Hypersil GOLD Hypersil GOLD C8 Hypersil GOLD Phenyl Hypersil GOLD Hypersil GOLD C8 Hypersil GOLD (1.9 m) Hypersil GOLD C8 (1.9 m) Hypersil GOLD (1.9 m) Hypersil GOLD C8 (1.9 m) Hypersil GOLD CN (1.9 m) Hypersil GOLD aQ Hypersil GOLD BioBasic 18 Hypersil GOLD C8 BioBasic 8 Hypersil GOLD CN BioBasic CN Hypersil GOLD Phenyl

inquire 72 72 130 130 133 72 76 86 72 76 72 76 72 76 84 78 72 91 76 93 84 96 86

16

To order or for customer support, please see back cover.

Column Selection

GC Column Selection
recommended. If the products differ primarily in their polarities, then a polar column such as the TR-WAX will be ideal. If you know the particular class of your sample, please refer to the column Selection by Application for a recommended phase (see page 19). Always select the least polar column which will perform the separation. B. Internal Diameter The selection of the internal diameter is often determined by the instrument or detection method. Most modern GC equipment will accommodate most column sizes. With a larger internal diameter, column sample capacity increases, but resolution and sensitivity decrease. Conversely, a smaller ID column can improve resolution and sensitivity, but with the drawback of reduced sample capacity and a greater need for sample preparation. It is a good idea to nd a similar application which gives separation of the desired components and use this as a guide. C. Film Thickness Increasing the lm thickness increases the sample capacity of the column and slows the elution of the peaks which can help when analyzing volatile compounds. A thicker lm also reduces the potential of overloading the column, thus improving the resolution. However, a thicker lm can be more sensitive to degradation. The same component will elute at a higher temperature on a thick lm when compared to a thin lm. Compounds with high boiling points or those with a high molecular weight should be analyzed using a thin lm to improve resolution and avoid unnecessarily long analysis times. Another factor to consider is the phase ratio () which is calculated using both the internal diameter and lm thickness in the following equation: =
Internal diameter (m) 4 x Film thickness (m)

2. To transfer an analysis from a column of one ID to another without changing the method substantially, choose a column with a similar value as this will have similar retention properties.
0.1 Film Thickness (m) 0.25 0.5 1 1.8 3

Internal Diameter (mm)

0.1 0.25 0.32 0.53

250 625 800 1325

100 250 320 530

50 125 160 265

25 63 80 133

14 35 44 74

8 21 27 44

When selecting a GC column for your analysis, it can often be difcult to choose the most appropriate column because of the wide range of options. However, the choice can be simplied by considering a number of questions about the planned separation. This section provides useful information to help you determine the most suitable column for your analysis.

Phase ratio () of common column dimensions

Column Selection for Existing or Regulated Methods

To select a TRACE GC column with an expanded temperature range and lower bleed levels, the chart on the following page provides a quick cross reference of TRACE columns to other GC columns. If you are following an ASTM, NIOSH or US EPA method, please refer to the column selection by method tables beginning on page 20, for the best Thermo Scientic alternative.

D. Column Length A longer column length will provide greater efciency and resolution, but this is not a linear relationship. Resolution is proportional to the square root of column length, so doubling the column length will increase resolution by approximately 40%. However, increasing the column length will also increase the retention time. Double column length, twice the analysis time. Generally, it is recommended to use the shortest column which will perform the desired separation.

Additional Considerations
Several generalizations regarding GC columns exist that you might rely on when in doubt. First, 95% of all GC columns used are either TR-1, TR-5 or TR-WAX type columns. A good starting column is a 30 m x 0.25 mm ID, 5% Phenyl column with a 0.25 m lm thickness, such as the TR-5. (260E142P; Page 51). This is a non-polar column, which separates predominately on boiling point, but has some polar characteristics. For further assistance in choosing the right column for your separation, please contact our technical support help desk.

Method Development Considerations

When rst developing a method, you should consider these column characteristics to determine the best column for the separation. A. Phase or chemistry B. Internal diameter C. Film thickness D. Length A. Column Phase In GC, the separation of two analytes occurs due to differences in their interaction with the stationary phase, therefore a phase must be chosen that matches the properties of the sample. For example, if the components have different boiling points (greater than 2 C), a non-polar column such as the TR-1 is

The phase ratio can be used in two ways: 1. To categorize the best dimensions for an application: a. For volatile samples < 100 b. For general samples ~ 250 c. For high molecular weight samples > 400

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17

GC and GC/MS

GC Column Selection by Manufacturer and Operating Temperatures

TRACE Column Phase Type Alternative To Maximum Operating Temperature

TR-1

100% Dimethyl Polysiloxane

DB-1, DB-Petro, BP1, HP-1, HP-1MS, Rtx-1, Ultra-1, SPB-1, SPB-1 Sulfur, Petrocol DH, CP-Sil 5CB, RSL-150, RSL-160, Zebron ZB-1, CB-1, OV-1, PE-1, 007-1(MS), SP-2100, SE-30, RH-1, CC-1, CP-Sil 5CB MS, VF-1ms DB-1, DB-Petro, BP1, HP-1, HP-1MS, Rtx-1, Rtx-1MS, Ultra-1, SPB-1, SPB-1Sulfur, Petrocol DH, CP-Sil 5CB, RSL-150, RSL-160, Zebron ZB-1, CB-1, OV-1, PE-1, 007-1(MS), SP-2100, SE-30 DB-5, BP5, Rtx-5, HP-5, Ultra-2, PTE-5, SPB5, MDN-5, CP-Sil 8CB, SPB-5, AT-5, MDN-5, Zebron ZB-5, 007-2(MPS-5), SE-52, SE-54 DB-5, DB-5ms, DB-5.625 XTI-5, BPX5, Rtx-5MS, Rtx-5, SilMS, AT-5, AT-5MS, 007-5MS, SPB-5, CP-Sil 8CB, Ultra-2, HP-5, HP-5MS, HP5-TA, SPB-5, MDN-5S, VF-5ms, RSL-200, CB-5, OV-5, PE-5, 007-2(MP-5), SE-52, SE-54, PTE-5, CC-5, RH-5ms, Zebron ZB-5 DB-35, DB-35ms, HP-35, HP-35MS, MDN-35,Rtx-35, Rtx-35MS, SPB-35, BPX35 DB-1701, Rtx-1701, HP-1701, BP10, OV-1701, 007-1701, CP-Sil 19 CB OV-17, SP-2250, DB-17, DB-17ms, DB-17ht, BPX50, Rtx-50, SPB-50, HP-50+, HP-17,AT50, RSL-300, PE-17, CC-17, 007-17(MPS-50), SPB-17, Zebron ZB-50 DB-Wax, BP20, Rtx-Wax, Stabilwax, HP20M, HP-Wax, HP-INNOWax, SUPELCOWAX-10, AT-Wax, Nukol, CP-Wax52CB, SUPEROX II, CB-WAX, PE-CW, CCWAX, 007-CW, CARBOWAX, HP-INNOWax, PE-WAX, Zebron ZB-WAX DB-Wax, Rtx-Wax, Stabilwax, HP20M, BP20, HP-Wax, HP-INNOWax, SUPELCOWAX 10, AT-Wax, Nukol, CP Wax 52CB, Zebron ZB-WAX DB-HT Sim Dis, DB-2887, BPX1, Rtx-2887, HP-1, Petrocol 2887, Petrocol EX2887 DB-23, BPX70, Rtx-2330, SP-2330, CP-Sil 88, SP-2380, HP-23, VF-23ms, 007-23, AT-Silar, PE-23 DB-624, BPX-Volatiles, Rtx-Volatiles, VOCOL56, OV-624, AT-624, HP-VOC, CP-Select624CB, 007-624, ZM-624

< 1.0 m; 340 C / 360 C for lms _ 300 C / 320 C for 1.5 to 3.0 m lms; 280 C / 300 C for lms > 3.0 m 370 C / 380 C

TR-1MS

100% Dimethyl Polysiloxane

TR-5 TR-5MS

5% Phenyl Methylpolysiloxane 5% Phenyl Polysilphenylene-siloxane

< 1.5 m; 340 C / 350 C for lms _ 280 C / 300 C for lms > 1.5 m 360 C / 370 C for lms less than 1.5 m; 350 C / 360 C for lms > 1.5 m

TR-35MS TR-1701 TR-50MS

35% Phenyl Polysilphenylene-siloxane 14% Cyanopropylphenyl Polysiloxane 50% Phenyl Polysilphenylene-siloxane

360 C / 370 C 280 C / 300 C 360 C / 370 C

TR-WAX

Polyethylene Glycol (PEG)

< 1.0 m; 260 C / 280 C for lms _ 240 C / 260 C for 1.0 m lms

TR-WaxMS

Polyethylene Glycol

280 C / 300 C

TR-SimDist TR-FAME TR-V1 TR-524 TR-525 TR-527 TR-5HT TR-8270 TR-DoA5 TR-DoA35 TR-BioDiesel (M) TR-BioDiesel (F) TR-BioDiesel (G) TR-Dioxin 5MS TR-PCB 8MS TR-8095

100% Dimethyl Polysiloxane 70% Cyanopropyl Polysilphenylene-siloxane Cyanopropylphenyl Polysiloxane Cyanopropylphenyl/dimethyl polysiloxane Proprietary 5% Phenyl Polysilphenylene-siloxane 5% Phenyl Polycarborane-siloxane 5% Phenyl Polysilphenylene-siloxane 5% Phenyl Methylpolysiloxane 35% Phenyl Polysilphenylene-siloxane 100% Dimethyl Polysiloxane Polyethylene Glycol 5% Phenyl Polysilphenylene-siloxane 5% Phenyl Polysilphenylene-siloxane 8% Phenyl Polysilphenylene-siloxane 8% Phenyl Polycarborane-siloxane

< 1.0 m; 400 C for lms _ 370 C for 2.65 m lms 250 C / 260 C 280 C / 300 C 240 C / 260 C 340 C / 360 C 330 C / 350 C 380 C / 400 C 330 C / 350 C 330 C / 350 C 330 C / 350 C 300 C / 320 C 280 C / 300 C 380 C / 400 C 330 C / 350 C 330 C / 350 C 360 C / 370 C

18

To order or for customer support, please see back cover.

Column Selection

GC Column Selection by Application

Recommended Alternative Acids Acid / Neutral Drugs Alcohols Alcohols in Beverages Aldehydes Alditol Acetates (Sugars) Amines - Aliphatic Amines - Aromatic Antidepressants Benzenes, substituted Biodiesel - Methanol Biodiesel - FAMEs Biodiesel - Glycerine Brominated Flame Retardants Butter Fat Carboxylic Acids Cigarette Lighter Fuel Chlorinated Aromatics Dioxins Drugs of Abuse Drugs of Abuse - THC Essential Oils Explosives FAMEs Glucose - Methylated Herbicides Hydrocarbons Ketones Monomers Nitroaromatics Organic acids Organochlorine Pesticides Organophosphorous Pesticides PAHs Parafns PCBs Pesticides Petroleum Phenols Phthalates Plant Sterols Polyethylene Polymers Polywax Pyrethroids Sedatives Semivolatiles Silicon Oil Solvents Terpenes Triglycerides TRPH Volatiles Xylenes
TR -1 ,T R1M TR S -5 ,T R5 TR M S -3 5M S TR -5 0M S TR -1 70 1 TR -W AX ,T TR RW -V ax 1 M S TR -F AM E TR -S im Di st TR -5 24 TR -5 25 TR -5 27 TR -5 HT TR -8 27 0 TR -D oA 5 TR -D oA 35 TR -B io Di es TR el -B (M io Di ) es TR e -B l( F) io Di es TR el -D ( G) io xi n TR 5M -P S CB 8M TR S -8 09 5

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19

GC and GC/MS

GC Column Selection by U.S. Pharmacopeia Specications

The USP specications are listed below with the appropriate Thermo Scientic GC column offerings included for your convenience. In some cases, there is more than one phase that matches the phase description. When in doubt, it is recommended that you consult the original complete method or contact our technical support team for additional information or help in choosing the correct column for your application.

USP Code Description

Recommended Thermo Scientic Phase

Page

G1 G2 G3 G5 G16 G20 G27 G36 G38 G42 G43 G46

Dimethylpolysiloxane oil Dimethylpolysiloxane gum 50% Phenyl-50% methylpolysiloxane 3-Cyanopropylpolysiloxane Polyethylene glycol compound (ave. mol. wt. ~15,000) with diepoxide linker Polyethylene glycol (ave. mol. wt. of 380 - 420) 5% Phenyl-95% methylpolysiloxane 1% Vinyl-5% phenylmethylpolysiloxane Phase G1 containing a small percentage of tailing inhibitor 35% Phenyl-65% dimethylpolysiloxane (percentages refer to molar substitution) 6% Cyanopropylphenyl-94% dimethylpolysiloxane (percentages refer to molar substitution) 14% Cyanopropylphenol-86% methylpolysiloxane

TRACE TR-1 TRACE TR-1MS TRACE TR-1 TRACE TR-1MS TRACE TR-50MS TRACE TR-FAME TRACE TR-WAX TRACE TR-WaxMS TRACE TR-WAX TRACE TR-WaxMS TRACE TR-5 TRACE TR-5MS TRACE TR-5 TRACE TR-5MS TRACE TR-1 TRACE TR-1MS TRACE TR-35MS TRACE TR-V1 TRACE TR-1701

49 50 49 50 55 60 56 57 56 57 51 52 51 52 49 50 53 59 54

20

To order or for customer support, please see back cover.

Column Selection

GC Column Selection by ASTM Method

Selected ASTM methods are listed below with the appropriate Thermo Scientic GC column offerings. In some cases, there is more than one phase or column dimension that can be used. When in
Method Title

doubt, it is recommended that you consult the original complete method or contact our technical support team for additional information or help in choosing the correct column for your application.
Recommended Thermo Scientic Phase Part Number Page

D1983 D2245 D2268 D2306 D2360 D2426 D2427 D2456 D2580 D2753 D2800 D2804 D2887 D2998 D2999 D3009 D3054 D3168 D3257 D3271 D3304 D3328 D3329 D3432 D3447 D3452 D3457 D3534 D3545 D3687 D3695 D3710 D3725 D3760 D3797 D3798 D3871 D3893 D3973 D4059 D4415 D4443 D4735

Fatty acid methyl ester composition Oils and oil acids in solvent-reducible paints High-purity n-heptane and isooctane C8 aromatic hydrocarbons Trace impurities in monocyclic aromatic hydrocarbons Butadiene dimer and styrene in butadiene concentrates Determination of C2 through C5 hydrocarbons in gasoline Polyhydric alcohols in alkyd resin Phenols in water Oil and oil acids FAME analysis Purity of methyl ethyl ketone Boiling range distribution of petroleum fractions Polyhydric alcohols in alkyd resin Monopentaerythritol in commercial pentaerythritol Composition of turpentine Cyclohexane Polymers in emulsion paints Aromatics in mineral spirits Solvent analysis in paints PCBs in environmental materials Comparison of waterborne petroleum oils Purity of methyl isobutyl ketone Unreacted toluene diisocyanates in urethane prepolymers and coating solutions Purity of halogentated organic solvents Identication of rubber FAME analysis PCBs in water Alcohol content and purity of acetate esters Alcohol content and purity of acetate esters Volatile alcohols in water by direct aqueous-injection GC Boiling range distribution of gasoline and gasoline fractions Fatty acids in drying oils Isopropylbenzene (cumene) o-Xylene p-Xylene Purgeable organic compounds in water using headspace sampling Purity of methyl amyl ketone and methyl isoamyl ketone Low molecular weight halogenated hydrocarbons in water PCBs in insulating liquids Dimer in acrylic acid Residual vinyl chloride monomer content in ppb range in homo- and co-polymers by headspace GC Trace thiophene in rened benzene

TR-WAX TR-FAME TR-1 TR-WAX TR-WAX TR-1 TR-1 TR-WAX TR-5MS TR-FAME TR-FAME TR-WAX TR-SimDist TR-SimDist TR-1 TR-1 TR-WAX TR-1 TR-1 TR-V1 TR-WAX TR-5MS TR-PCB 8MS TR-1 TR-WAX TR-V1 TR-1 TR-V1 TR-1 TR-FAME TR-5MS TR-PCB 8MS TR-V1 TR-WAX TR-WAX TR-SimDist TR-SimDist TR-FAME TR-WAX TR-1 TR-WAX TR-WAX TR-V1 TR-V1 TR-V1 TR-5MS TR-PCB 8MS TR-WAX TR-V1 TR-WAX

260W142P 260M154P inquire 260W154P 260W155P 260A470P 260A470P 260W298P 260F223P 260M154P 260M154P 260W298P 260S348P 260S347S 260A297P inquire 260W224P inquire 260A297P 260V396P 260W298P 260F154P 26AJ148P 260A395P 260W298P 260V396P 260A309P 260V396P 260A309P 260M154P 260F336P 26AJ148P 260V396P 260W298P 260W298P 260S348P 260S347S inquire 260W155P inquire 260W236P 260W236P 260V408P 260V396P 260V396P 260F154P 26AJ148P 260W143P 260V396P 260W225P

56 60 49 56 56 49 49 60 52 60 60 56 58 58 49 49 56 49 49 59 56 52 61 49 56 59 49 59 49 60 52 61 59 56 56 58 58 60 56 49 56 56 59 59 59 52 61 56 59 56

www.thermo.com /columns

21

GC and GC/MS

GC Column Selection by ASTM Method

Method Title Recommended Thermo Scientic Phase Part Number Page

D4773 D4806 D4815 D4864 D4947 D5060 D5075 D5134 D5135 D5399 D5441 D5442

Propylene gycol monomethyl ether, dipropylene glycol monomethyl ether, and propylene glycol monomethyl ether acetate Denatured fuel ethanol for blending with gasoline for use as automotive spark-ignition engine fuel MTBE, ETBE, TAME, DIPE, tert-Amyl Alcohol and C1 to C4 Alcohols in gasoline Traces of methanol in propylene concentrates Chlordane and heptachlor in indoor air Impurities in high-purity ethylbenzene Nicotine in indoor air Petroleum naphthas through n-nonane Styrene Boiling point distribution of hydrocarbon solvents Methyl t-butyl ether Petroleum waxes

TR-5MS TR-1 TR-1 TR-5MS TR-5 TR-5MS TR-5MS TR-WAX TR-5MS TR-1 TR-WAX TR-SimDist TR-1 TR-1 TR-5 TR-5MS

260E470P inquire 260A396P inquire 260E334P 260F336P 260F298P 260W236P 260F297P inquire 260W236P 260S348P inquire 260A143P 260E130P 260F130P inquire inquire 260A470P 260A308P inquire inquire 260F142P 260A308P 260A309P 260V333P 260F142P 260Q142P 260R142P 260W155P 260M154P inquire 260F142P 260S250P inquire 260S250P inquire 26AF024P inquire 260E296P 260F284P 260V408P 260E470P inquire 260W309P 260S250P

52 49 49 52 51 52 52 56 52 49 56 58 49 49 51 52 52 49 49 49 49 49 52 49 49 59 52 54 52 56 60 49 52 58 50 58 49 61 49 51 52 59 51 49 56 58

D5480 D5501 D5580 D5599 D5623 D5713 D5739 D5769

Motor oil volatility Ethanol content of denatured fuel ethanol Benzene, toluene, ethylbenzene, p/o/m-xylene, C9 and heavier aromatics in nished gasoline Oxygenates in gasoline by oxygen selective FID Sulfur compounds in light petroleum liquids using sulfur selective detection High purity benzene for cyclohexane feedstock Oil spill source identication using positive ion electron impact low resolution MS Benzene, toluene and total aromatics in nished gasolines

TR-5MS TR-1 TR-1 TR-1 TR-1 TR-1 TR-5MS TR-1 TR-1 TR-V1

D5790 D5812

Purgeable organic compounds in water Organochlorine pesticides in water

TR-5MS TR-1701 TR-5MS TR-WAX

D5917 D5974 D5986 D6160 D6352 D6417 D6584 D6729 D6730

Trace impurities in monocyclic aromatic hydrocarbons Fatty androsin acids in tall oil fraction products Oxygenates, benzene, toluene, C8-C12 aromatics and total aromatics in nished gasoline by GC/FTIR PCBs in waste materials Boiling range distribution of petroleum fractions Engine oil volatility Free and Total Glycerin in B-100 Biodiesel Individual components in spark ignition engine fuels Individual components in spark ignition engine fuels using precolumn

TR-FAME TR-1 TR-5MS TR-SimDist TR-1MS TR-SimDist TR-1 TR-Biodiesel G TR-1 TR-5 TR-5MS TR-V1

E202 E475 E1616 E1863

Ethylene glycols and propylene glycols Di-tert-butyl peroxide Acetic anhydride Acrylonitrile

TR-5 TR-1 TR-WAX TR-SimDist

22

To order or for customer support, please see back cover.

Column Selection

GC Column Selection by US EPA Drinking Water Test Method

Selected EPA Drinking Water methods are listed below with the appropriate Thermo Scientic GC column offerings. In some cases, there is more than one phase or column dimension that can be used.
Method Title

When in doubt, it is recommended that you consult the original complete method or contact our technical support team for additional information or help in choosing the correct column for your application.
Recommended Thermo Scientic Phase Part Number Page

501.3 502.1 502.2 503.1 504 504.1 506 507 509 513 515.2 524.1

Trihalomethanes Volatile halogenated compounds Volatile organic compounds Volatile aromatic & unsaturated organics EDB and DBCP EDB and DBCP Phthalates and adipates Organonitrogen and organophosphorus pesticides Ethylene thiourea Dioxin Chlorinated herbicides Volatile organic compounds

TR-V1 TR-V1 TR-5MS TR-V1 TR-V1 TR-V1 TR-5MS TR-5MS TR-5MS TR-5MS TR-5MS TR-1 TR-5MS TR-5MS TR-50MS TR-1701 TR-WAX TR-5MS TR-5MS TR-50MS TR-524 TR-V1 TR-V1

260V396P 260V408P 260F396P 260V408P 260V332P 260V408P 260F396P 260F396P 260F224P 260F396P 260F224P 260A143P 260F143P 260F142P 260R142P 260Q130P 260W130P 260F154P 260F143P 260R143P 26RV495P 260V408P 260V332P 26RV495P 260V408P 260V332P 26RX142P 260F142P 26RX142P 260F142P 26RF142P 260F142P 260A143P 260F142P 260F142P 260Q224P 260Q143P 260C143P 260Q143P 260C143P

59 59 52 59 59 59 52 52 52 52 52 49 52 52 55 54 56 52 52 55 61 59 59 61 59 59 61 52 61 52 61 52 49 52 52 54 54 53 54 53

524.2

Volatile organic compounds

TR-524 TR-V1 TR-V1

525.1 525.2 527 548.1 551 552 552.1

Semi-volatile organic compounds Semi-volatile organic compounds Selected pesticides and ame retardants Endothall Chlorinated disinfection by-products/chlorinated solvents Haloacetic acids Haloacetic acids and dalapon

TR-525 TR-5MS TR-525 TR-5MS TR-527 TR-5MS TR-1 TR-5MS TR-5MS TR-1701 TR-1701 TR-35MS TR-1701 TR-35MS

www.thermo.com /columns

23

GC and GC/MS

GC Column Selection by US EPA Waste Water Test Method

Selected EPA Waste Water methods are listed below with the appropriate Thermo Scientic GC column offerings. In some cases, there is more than one phase or column dimension that can be used.
Method Title

When in doubt, it is recommended that you consult the original complete method or contact our technical support team for additional information or help in choosing the correct column for your application.
Recommended Thermo Scientic Phase Part Number Page

601 602 603 604 606 607 608.1 608.2 609 610 611 612 613 614 614.1 615 616 617 618 619 620 622 622.1 624 625 627 630.1 633 633.1 634 645 646

Purgable halocarbons Purgable aromatics Acrolein and acrylonitrile Phenols Phthalate ester Nitrosamines Organochlorine pesticides in industrial and municipal water Organochlorine pesticides in wastewater Nitroaromatics and isophorone Polynuclear aromatic hydrocarbons Haloethers Chlorinated hydrocarbons Dioxin Organophosphorous pesticides in industrial and municipal water Organophosphorous pesticides in wastewater Chlorinated herbicides in industrial and municipal water C, H, and O compounds Organohalide pesticides and PCBs in industrial and municipal water Volatile pesticides in industrial and municipal water Triazines, pesticides and PCBs in industrial and municipal water Diphenylamine in industrial and municipal water Organophosphorous pesticides in industrial and municipal water Thiophosphate pesticides Purgeables Base/neutrals and acids Dinitroaniline pesticides in industrial and municipal water Dithiocarbamate pesticides as carbon disulde Organonitrogen pesticides Neutral nitrogen-containing pesticides Thiocarbamate pesticides Amine pesticides and lethane in industrial and municipal water Dinitro aromatic pesticides

TR-V1 TR-V1 TR-V1 TR-5MS TR-V1 TR-5MS TR-5MS TR-35MS TR-5MS TR-35MS TR-5MS TR-35MS TR-5MS TR-5MS TR-5MS TR-35MS TR-5MS TR-5MS TR-35MS TR-5MS TR-35MS TR-5MS TR-5MS TR-50MS TR-5MS TR-50MS TR-5MS TR-50MS TR-1 TR-5MS TR-5MS TR-1 TR-5MS TR-35MS TR-1 TR-5MS TR-5MS TR-50MS TR-5MS TR-35MS TR-V1 TR-V1 TR-5MS TR-5MS TR-5MS TR-35MS TR-5MS TR-5MS TR-5MS TR-50MS TR-5MS TR-35MS TR-5MS TR-35MS TR-5MS TR-35MS TR-5MS TR-35MS

260V408P 260V332P 260V408P 260F296P 260V408P 260F296P 260F142P 260C142P 260F142P 260C142P 260F142P 260C142P 260F224P 260F224P 260F143P 260C143P 260F142P 260F143P 260C143P 260F143P 260C143P 260F154P 260F142P 260R142P 260F142P 260R142P 260F142P 260R142P 260A142P 260F142P 260F142P 260A224P 260F224P 260C143P 260A143P 260F143P 260F142P 260R142P 260F142P 260C142P 260V408P 260V332P 260F142P 260F143P 260F143P 260C143P 260F142P 260F143P 260F142P 260R142P 260F142P 260C142P 260F142P 260C142P 260F142P 260C142P 260F142P 260C142P

49 49 49 52 59 52 52 53 52 53 52 53 52 52 52 53 52 52 53 52 53 52 52 55 52 55 52 55 49 52 52 49 52 53 49 52 52 55 52 53 59 59 52 52 52 53 52 52 52 55 52 53 52 53 52 53 52 53

24

To order or for customer support, please see back cover.

Column Selection

GC Column Selection by US EPA Solid Waste Test Method

Selected EPA Solid Waste methods are listed below with the appropriate Thermo Scientic GC column offerings. In some cases, there is more than one phase or column dimension that can be used.
Method Title

When in doubt, it is recommended that you consult the original complete method or contact our technical support team for additional information or help in choosing the correct column for your application.
Recommended Thermo Scientic Phase Part Number Page

8010B 8011 8015B 8020A 8021A 8030A 8031 8032 8040A 8060 8061 8070 8081 8090 8095 8100 8110 8120A 8121 8140 8141A 8150B 8151 8240B 8250A 8260A 8270B 8270C 8280 8290

Halogenated volatile organics EDB and DBCP Nonhalogenated volatile organics Aromatic volatile organics Halogenated and aromatic volatile organics Acrolein and acrylonitrile Acrylonitrile Acrylamide Phenols Phthalate esters Phthalate esters Nitrosamines Organochlorine pesticides and PCBs Nitroaromatics and cyclic ketones Explosives Polynuclear aromatic hydrocarbons Haloethers Chlorinated hydrocarbons Chlorinated hydrocarbons Organophosphorous pesticides Organophosphorous pesticides Chlorinated herbicides Chlorinated herbicides Volatile organic compounds Semi-volatile organic compounds Volatile organic compounds Semi-volatile organic compounds Semi-volatile organic compounds Polychlorinated dioxins and furans Polychlorinated dioxins and furans

TR-V1 TR-V1 TR-5MS TR-V1 TR-5MS TR-V1 TR-5MS TR-V1 TR-5MS TR-V1 TR-V1 TR-V1 TR-5MS TR-35MS TR-5MS TR-5MS TR-5MS TR-5MS TR-5MS TR-5MS TR-8095 TR-5MS TR-5MS TR-5MS TR-5MS TR-5MS TR-5MS TR-5MS TR-5MS TR-5MS TR-5MS TR-5MS TR-5MS TR-V1 TR-V1 TR-5MS TR-5MS TR-V1 TR-V1 TR-5MS TR-5MS TR-8270 TR-5MS TR-5MS

260V408P 260V332P 260F142P 260V408P 260F296P 260V408P 260F296P 260V408P 260F296P 260V408P 260V339P 260V339P 260F142P 260C142P 260F142P 260F142P 260F143P 260F223P 260R142P 260F143P 260P123P 260F142P 260F142P 260F143P 260F143P 260F142P 260R142P 260F142P 260R142P 260F142P 260R142P 260F142P 260R142P 260V408P 260V332P 260F142P 260F143P 260V408P 260V332P 260F142P 260F143P 26RF296P 260F154P 260F154P

59 59 52 59 52 59 52 59 52 59 59 59 52 53 52 52 52 52 52 52 61 52 52 52 52 52 52 52 52 52 52 52 52 59 59 52 52 59 59 52 52 61 52 52

www.thermo.com /columns

25

GC and GC/MS

GC Column Selection by NIOSH Method

Selected NIOSH methods are listed below with the recommended Thermo Scientic GC column offerings included for your convenience. There may be more than one phase or column dimension that can be
Method Title

used. When in doubt, it is recommended that you consult the original complete method or contact our technical support team for additional information or help in choosing the correct column for your application.
Recommended Thermo Scientic Phase Part Number Page

801 1001 1002 1003 1004 1005 1010 1011 1013 1015 1016 1018 1020 1300 1301 1302 1400 1401 1402 1403 1450 1451 1453 1454 1457 1458 1500 1501 1550 1551 1552 1601 1602 1604 1606 1608 1609 1610 1611 1612 1613 1614 1615 1618 2000 2004 2005 2007 2010 2012

Aerobic bacteria Methylene chloride Chloroprene Halogenated hydrocarbons Dichloroethyl ether Methylene chloride Epichlorohydrin Ethyl bromide Propylene dichloride Vinylidene chloride 1,1,2,2-Tetrachoro-2,2-diuoroethane and 1,1,2,2-tetrachoro-1,2-diuoroethane Dichlorodifuoromethane, 1,2-dichlorotetrauoroethane and chlorodiuoromethane 1,1,2-Trichloro-1,2,2-triuoroethane Ketones 1 Ketones 2 N-Methyl-2-pyrrolidinone Alcohols 1 Alcohols 2 Alcohols 3 Alcohols 4 Esters 1 Methyl cellosolve acetate Vinyl acetate Isopropyl acetate Ethyl acetate Methyl acetate Hydrocarbons (b.p. 36 - 126 C) Aromatic hydrocarbons Naphthas Turpentine Terpenes 1,1-Dichloro-1-nitroethane Dioxane Acrylonitrile Acetonitrile Glycidol Tetrahydrofuran Ethyl ether Methylal Propylene oxide Pyridine Ethylene oxide Methyl-tert-butyl ether Isopropyl ether Methanol Dimethlyacetamide and dimethylformamide Nitroaromatics Aminoethanol compounds 1 Aliphatic amines n-Butylamine

TR-FAME TR-1 TR-1 TR-V1 TR-1 TR-WAX TR-WAX TR-WAX TR-WAX TR-V1 TR-WAX TR-1 TR-WAX TR-WAX TR-WAX TR-5 TR-WAX TR-WAX TR-WAX TR-WAX TR-WAX TR-5 TR-5 TR-1 TR-WAX TR-WAX TR-1 TR-WAX TR-1 TR-1 TR-WAX TR-1 TR-5 TR-WAX TR-WAX TR-WAX TR-WAX TR-1 TR-WAX TR-5 TR-5 TR-WAX TR-1 TR-1 TR-35MS TR-WAX TR-5 TR-5 TR-5 TR-5

inquire 260A143P 260A296P 260V339P inquire 260W143P inquire 260W224P inquire inquire 260W224P 260A297P 260W143P 260W224P 260W224P 260E297P 260W224P 260W224P 260W224P 260W143P 260W224P 260E297P 260E297P 260A297P 260W297P 260W297P 260A395P 260W297P 260A154P 260A154P 260W310P inquire 260E297P 260W224P 260W297P inquire 260W224P 260A297P inquire 260E297P 260E297P inquire 260A224P 260A395P 260C298P 260W224P 260E225P inquire 260E142P 260E142P

60 49 49 59 49 56 56 56 56 59 56 49 56 56 56 51 56 56 56 56 56 51 51 49 56 56 49 56 49 49 56 49 51 56 56 56 56 49 56 51 51 56 49 49 53 56 51 51 51 51

26

To order or for customer support, please see back cover.

Column Selection

Method

Title

Recommended Thermo Scientic Phase

Part Number

Page

2017 2500 2505 2520 2529 2536 2537 2541 2542 2546 2549 2550 2551 3511 3513 5020 5515 5519 5523 5600 5602 5701 9200 9201

Aniline, o-toluidine and nitrobenzene Methyl ethyl ketone Furfuryl alcohol Methyl bromide Furfural Valeraldehyde Methyl methacrylate Formaldehyde Mercaptans Cresols and phenol Volatile organic CPDS (screening) Benzothiazole in asphalt fume Nicotine Monomethylaniline Tetranitromethane Dibutyl phthalate and di(2-ethylhexyl) phthalate Polynuclear aromatic hydrocarbons Endrin Glycols Organophosphorus pesticides Chlorinated organonitrogen herbicides (air sampling) Resorcinol Chlorinated organonitrogen herbicides (hand wash) Chlorinated organonitrogen herbicides (dermal patch)

TR-5 TR-1 TR-1 TR-1 TR-5 TR-5 TR-35MS TR-WAX TR-1 TR-WAX TR-1 TR-1 TR-5 TR-5 TR-1 TR-1 TR-1 TR-1 TR-35MS TR-5 TR-50MS TR-1 TR-50MS TR-50MS

260E297P 260A297P 260A142P 260A297P 260E296P 260E131P 260C298P 260W224P 260A296P 260W143P 260A296P 260A297P 260E297P 260E142P 260A297P 260A130P 260A309P 260A309P 260C298P 260E297P 260R142P 260A142P 260R142P 260R142P

51 49 49 49 51 51 53 56 49 56 49 49 51 51 49 49 49 49 53 51 55 49 55 55

www.thermo.com /columns

27

GC and GC/MS

Notes

28

To order or for customer support, please see back cover.