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Protein molecular weight on SDS-PAGE is usually estimated by plotting Rf values versus log molecular weight and is calculated using a linear equation. We found that migration of proteins on a variety of SDS-PAGE gels is more accurately calculated using non-linear equations. The HiMark Molecular Weight Calculator allows you to easily and accurately calculate the molecular weight of your proteins on NuPAGE Novex 3-8% or 7% Tris-Acetate Gels using the standard curve obtained with HiMark . To use the calculator, click on the Calculator tab.
HiMarkTM Unstained HMW Standard Protein band MW(Da) log10(MW) 500 kDa 500,000 5.6990 290 kDa 290,000 5.4624 240 kDa 240,000 5.3802 160 kDa 160,000 5.2041 116 kDa 116,000 5.0645 97 kDa 97,000 4.9868 66 kDa 66,000 4.8195 55 kDa 55,000 4.7404 40 kDa 40,000 4.6021
Unknown
Protein MW(Da) log10(MW) Rf
Whole Range
5.8000 5.6000 5.4000 5.2000
Log MW
0.200
0.400
0.600
0.800
1.000
1.200
Rf
High MW Range
5.8000 5.7000 5.6000 5.5000
Log MW
0.020
0.040
0.060
0.080
0.100
0.120
0.140
0.160
0.180
0.200
0.220
Rf
Low MW Range
5.1000
5.0000
4.9000
Log MW
4.8000
4.7000
4.6000
4.5000 0.000
0.050
0.100
0.150
0.200
0.250
0.300
0.350
0.400
0.450
0.500
0.550
0.600
Rf