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# Mark sheme bio 3 trial perak 2007

Biology
Paper 3
Marking Scheme

## Question Score Explanation Remarks

1(a) KB0603 - Measuring and using numbers
3 Able to record all four heights of the coloured
liquid correctly and accurately.

Temperature, oC Height, cm
20 2.6
30 3.9
40 4.0
50 2.5
2 Able to record three heights of the coloured
liquid correctly and accurately.
1 Able to record two heights of the coloured liquid
correctly and accurately.
0 No response or wrong response

## Question Score Explanation Remarks

1(b)(i) KB0601 - Observing
3 Able to state the two observations correctly
based on the following criteria:
C1: The temperature of the water bath and the
height of the coloured liquid in the
manometer for that particular temperature
or
C2: Comparing the height of the coloured
liquid for two different temperatures
1. When the temperature of water bath is
20oC, the increase in height of coloured
liquid is 2.6cm
2. When the temperature of water bath is
30oC, the increase in height of coloured
liquid is 3.9cm
3. The increase in height of the coloured
liquid at 30oC is higher than the height at
20oC // vice versa
2 Able to state any one observation correctly (as
above) and any one observation less accurate
1. When the temperature of water bath is Temperature is
20oC, the increase in height of coloured correct but the
liquid is low increase in height is
2. When the temperature of water bath is less accurate
40oC, the increase in height of coloured
liquid is high
Mark sheme bio 3 trial perak 2007

## Question Score Explanation Remarks

1(b)(i) 1 Able to state any one observation correctly or
any two observations less accurate or two ideas
of observation.
1. At low temperature, the increase in height
of coloured liquid is low
2. At high temperature, the increase in
height of coloured liquid is high

## 1(b)(ii) KB0604 - Making inferences

3 Able to state two observation , correctly and
accurately based on the following criteria:
C1: Increase in height of the coloured liquid is
caused by the rate of respiration of yeast, or
C2: The difference in the increase in height of
the coloured liquid is due to the different
rate of respiration of yeast
1. At 20oC, the rate of respiration of yeast is
low
2. At 40oC, the rate of respiration of yeast is
very high
3. The rate of respiration of yeast is higher
at 30oC than the rate of respiration of
yeast at 20oC // vice versa
2 Able to state any one inference correctly which
correspond to the observation made and any one
inference less accurate for the observation made
1 Able to state any one inference correctly which
correspond to the observation made or two ideas
of inference for the two corresponding
1. Respiration is low
2. Increase in height of coloured liquid is
due to respiration
0 No response or wrong response
Mark sheme bio 3 trial perak 2007

## Question Score Explanation Remarks

1(c) KB0610 - Controlling variables
Able to state all the variables correctly
V1 Manipulated variable:
Temperature of water bath

V2 Responding variable:
Height of the coloured liquid / Rate of respiration

V3 Controlled variable:
Volume of the yeast suspension used / Duration
of yeast suspension in the water bath before the
experiment starts

## Able to state method to handle variable correctly

M1
Manipulated variable:
Set up different temperatures of the water bath,
20oC, 30oC, 40oC and 50oC
M2
Responding variable:
Measure the height of the coloured liquid using a
ruler / Calculate the rate of respiration using the
formula of height divided by time taken

M3 Controlling variable:
Use 15cm3 of yeast suspension / Leave the yeast
suspension in the water bath for 5 minutes before
the experiment starts
3 Able to state all V and M
2 Able to state 4 -5 V and M correctly
1 Able to state 2-3 V and M correctly
0 No response or wrong response
Mark sheme bio 3 trial perak 2007

## Question Score Explanation Remarks

1(d) KB0611 - Making hypothesis
3 Able to state a hypothesis correctly based on the
three complete criteria:
C1: State the manipulated variable
C2: State the responding variable
C3: State a specific relationship / showing
direction of relationship between C1 and C2

1. When the temperature (of the water bath)
increases until optimum temperature, the
higher increase in height of the coloured
liquid / the rate of respiration increases //
2. When the temperature increases above
the optimum temperature, the lower
increase in height of coloured liquid / the
rate of respiration decreases

## 2 Able to state a hypothesis correctly based on two

complete criteria
1 Able to state a hypothesis correctly based on one
complete criteria
0 No response or wrong response

## 1(e)(i) KB0606 - Communicating

3 Able to construct one table containing the
following criteria
C1: State the title with units correctly
C2: Transfer all data of temperature and increase
in height correctly
C3: State at least three values for rate of
respiration correctly
Temperature, Increase in Rate of
o
C height of respiration,
coloured cm min-1
liquid, cm
20 2.6 0.26
30 3.9 0.39
40 4.0 0.40
50 2.5 0.25
2 Able to construct one table containing two
criteria correctly
1 Able to construct one table containing one
criteria correctly
0 No response or wrong response
Mark sheme bio 3 trial perak 2007

## Question Score Explanation Remarks

1(e)(ii) KB0608 - Interpreting data
3 Able to state the relationship between the
temperature, the increase in height of coloured
liquid and the rate of respiration correctly based
on the following criteria
C1: State the increase in temperature until/above
the optimum temperature
C2: State the amount of increase in height of
coloured liquid
C3: State the rate of respiration
When the temperature increases until the
optimum temperature, the increase in height of
colour liquid is higher because the rate of
respiration is higher
2 Able to state the relationship correctly based on
two criteria
1 Able to state correctly one criterion
0 No response or wrong response

KB0605 - Predicting
1(f) 3 Able to predict the observation and the value of
increase in height of the coloured liquid and
explain the prediction based on the following
criteria
C1: Observation - The increase in height of the
coloured liquid
C2: Value - less than 2.5cm
C3: Explanation - The rate of respiration is lower
/ Enzyme denature
The height of the coloured liquid will increase
but less than 2.5cm because the rate of
respiration is lower / denaturation of respiratory
enzyme
2 Able to make a prediction correctly based on two
criteria
1 Able to make a prediction correctly based on one
criterion
0 No response or wrong response
Mark sheme bio 3 trial perak 2007

## Question Score Explanation Remarks

1(g) KB0607 - Using space-time relationship
3 Able to state the changes in the increase in height
of the coloured liquid with time and give the fact
and an explanation for the changes correctly
based on the following criteria:
C1: Observation - The changes in the increase in
height of the coloured liquid with time
C2: Fact - Respiration has stopped
C3: Explanation - Depletion of glucose / Death
of yeast
As time goes by, the height of the coloured liquid
will continue to increase above 2.6cm and
eventually stop. The respiration has stopped
because the glucose has been depleted / the yeast
has died
2 Able to state the changes in the increase in height
of the coloured liquid with time correctly based
on two criteria, C1 and C2 or C1 and C3
1 Able to state the changes in the increase in height
of the coloured liquid with time correctly, C1
0 No response or wrong response

## 1(h) KB0609 - Defining operationally

3 Able to state correctly and accurately the
definition of the rate of respiration based on the
results of the experiment using the following
criteria
C1: An activity of microorganism
C2: Increase in height of coloured liquid
C3: the rate is influenced by the temperature

Respiration is an activity of microrganisms
which caused the increase in height of coloured
liquid in the manometer and the rate is
influenced by the temperature
2 Able to state the definition of the rate of
respiration based on two criteria, C1 and C2 or
C1 and C3
1 Able to state the definition of the rate of
respiration based on C1 or able to give an idea of
a definition
0 No response or wrong response
Mark sheme bio 3 trial perak 2007

## 1(i) KB0602 - Classifying

3 Able to complete Table 1.3 correctly by using all
the abiotic components and the activities of
microorganism given and classifying them into
manipulated and responding variables

Variable
Manipulated Responding
pH Growth

## Light intensity Photosynthesis

Concentration of Reproduction
nutrients
2 Able to state any two pairs of manipulated and
responding variables correctly
1 Able to state any one pair of manipulated and
responding variables correctly
0 No response or wrong response

## 2. [KB061201 – Statement of Identified Problem]

Score Criteria
3 Able to give a statement of identified problem completely and correctly based on
the following criteria
C1: state the manipulated variable
C2: state the responding variable
C3: Relationship between the two variables in the question form
1. How does the state / different speed of air movement affect the rate of
transpiration?
2. What are the effect of the state / different speed of air movement on the
rate of transpiration?
2 Able to give a statement of identified problem based of any two criteria correctly
or one criterion less accurate
1. How do the state / different speed of air movement affect transpiration?
2. What is the effect of air movement on the rate of transpiration?
3. Different speed of air movement influence the rate of transpiration
1 Able to give a statement of identified problem based on one criterion correctly or
two criteria less accurate or idea of the identified problem
Mark sheme bio 3 trial perak 2007

## 1. How does air movement affect transpiration?

2. Air movement influence transpiration
0 No response or wrong response

## 2 [KB061202 – Making hypothesis]

Score Criteria
3 Able to state a hypothesis correctly based on the three complete criteria:
C1: State the manipulated variable
C2: State the responding variable
C3: State a specific relationship / showing direction of relationship between C1
and C2
1. As the speed of the air movement increases, the rate of transpiration
increases
2. The rate of transpiration is higher in a moving air than in a still air
2 Able to state a hypothesis based on two complete criteria or one criterion less
accurate
1. As the speed of the air movement increases, the transpiration increases
2. The transpiration is higher in a moving air than in a still air
1 Able to state a hypothesis based on one complete criterion or two criteria less
accurate or idea of hypothesis
1. Different speed of air movement affect transpiration
2. Air movement affects transpiration.
0 No response or wrong response

## 2 [KB061203 – Planning an investigation]

Score Criteria
3 Able to state 7-9 aspects in planning an investigation of the following:
P1: Statement of identified problem
P2: Objective of study
P3: Variables
P4: Statement of hypothesis
P5: List of materials and apparatus
P6:Techinique used
P7: Experimental procedure
P8: Presentation of data
P9: Conclusion
2 Able to state 4-6 aspects in planning an investigation
1 Able to state 1-3 aspects in planning an investigation
0 No response or wrong response

## 2. [KB061203 – Objective of study]

Criteria
Mark sheme bio 3 trial perak 2007

## Able to state the objective of study

To study the effect of air movement on the rate of transpiration

## 2. [KB061203 – Controlling variables]

Criteria
Able to state all the variables correctly
i. Manipulated - State / speed of air movement
ii. Responding - Distance traveled by air bubble / Rate of transpiration
iii. Controlled - Time for the movement of air bubble // Temperature // Type of plant used

## 2. [KB061203 – Technique used]

Criteria
Able to state the technique used based on the following criteria
C1: What data - distance traveled // Rate of transpiration
C2: How to collect - Measure and record // Calculate
C3: Instrument used - using a ruler // using formula of distance traveled divided by time taken
1. Measure and record the distance travelled (in a given time) using a ruler
2. Calculate the rate of transpiration using the formula of distance travelled divided by
time taken

## 2. [KB061203 – Presentation of data]

Criteria
Able to construct one table containing the following aspects
1. State of air movement
2. Time taken for air bubble to travel from X to Y with unit
3. Rate of transpiration with unit

## State of air Time taken for air bubble to travel Rate of

movement from X to Y, min transpiration,
cm min-1
1 2 3 Average
Moving air
Still air

2. [KB061203 – Conclusion]
Criteria
Mark sheme bio 3 trial perak 2007

## Able to state a conclusion correctly

1. When the speed of the air movement increases, the rate of transpiration increases
2. The rate of transpiration is higher in a moving air than in a still air

2 [KB061204 – Procedure]
Score Criteria
3 Able to state 5 criteria of the following
P1 : Preparation of specimen and apparatus / potometer (at least 4S to get a tick)
S1: Choose a leafy shoot
S2: Cut the stem obliquely
S3: Insert the rubber tube into capillary tube
S4: Insert the cut stem to the rubber tube
S5: Set up the apparatus in upright position
S6: Introduce the air bubble

## P2 : Handling the manipulated variable

Fan switch on and fan switch on
P3 : Handling the controlled variable
Measure the fixed distance and mark X and Y
P4 : Handling responding variable / Collecting and recording data
- activate the stop watch at X
- stop the stop watch at Y
- record the time in the table
P5 : Accuracy of the data obtained / Precaution
- repeat the step and find the average
- smear with vaseline to prevent leakage / wipe dry the leaves and apparatus

## 1. Choose and cut off a leafy shoot from a plant.

2. Immerse the cut end immediately into a basin of water
3. Cut 1cm of the bottom of the stem obliquely under water using a sharp
4. Measure a distance of 10 cm on the capilary tube and mark the point X and
point Y
5. Insert one end of the rubber tube into the capillary tube fill the tube with
water
6. Insert the other end of the rubber tube with the cut end of the stem under
water
7. Set up the leafy shoot and capillary tube in the upright position using retort
stand and clamp with the other end of the capillary tube immerses in a
beaker of water
8. Wipe dry the leaves and apparatus using dry cloth
9. Smear all the joints of the apparatus with vaseline to prevent leakage
10. Place the apparatus under the fan which is switched off.
11. Introduce an air bubble into the capillary tube by lifting the end of the
capillary tube out of the beaker for a short while and then returned it to the
beaker again
12. Allow the air bubble to move until it reaches the X mark and activate the
stop watch
13. Stop the stop watch when the air bubble reaches the Y mark
Mark sheme bio 3 trial perak 2007

14. Record the time taken for the air bubble to travel from X to Y in the table
provided.
15. Repeat steps 7 to 9 to obtain an average reading
16. Repeat the above steps by switching on the fan /using different speeds of
the fan (speed 1, 2, 3, 4, 5) and record the result
2 Able to state 4 criteria
1 Able to state 2-3 criteria
0 No response or wrong response

## 2 [KB061205 – List of materials and apparatus]

Score Criteria
3 Able to list all the materials and apparatus so that the experiment can function
and the data can be obtained:

Apparatus
Capillary tube, rubber tube, stop watch, ruler, beaker, fan, retort stand with clamp

Materials
Plant shoot, water, vaseline

## 2 Able to list the materials and apparatus listed below

Apparatus
Capillary tube, rubber tube, stop watch, beaker, fan

Materials
Plant shoot, water, (vaseline)

## 1 Able to list the materials and apparatus listed below

Apparatus
Capillary tube, stop watch, fan

Materials
Plant shoot, water, (vaseline)
0 No response or wrong response

Extra 2 marks

Score Criteria
1 B1 - able to state the technique used based on the given criteria
1 B2 - able to construct a correct table for presenting the data