Department Course number Course title Credit hours

Clinical Laboratory Sciences : : CLS 332 Instrumental Analysis

3+1=4

Course description: The knowledge of the principles of instrumentation is essential for a clinical laboratory technologist which can be utilized data. A good understanding of the principles of instrumentation along with the fundamentals of electronics applicable to laboratory instruments is offered in the course. This will prepare the laboratory technologist to adopt analytical procedures to the instruments of the future.

CLS 332 ee!s 1

Lecture Schedule Sub"ect

Introduction Electromagnetic radiation Absorption spectroscopy Spectrophotometric techniques Application of eer!s law "isible absorption spectrophotometer# $omponents and applications Atomic absorption spectrophotometer &rinciple# basic components and their function 'lamless atomic absorption spectrophotometer Ad(antages# interferences Emission spectroscopy *uminescence 'luorometers 'luorometers (+s con(entional spectrophotometers Instrumentation &ossible disad(antages# solutions Special application of fluorometers *ight scattering phenomenon Turbidity and nephlometery -ephlomter (+s spectrophotometer 'lame photometry Internal standard in flame photometry Ad(antages+disad(antages 0sometry 0smosis# colligati(e properties 0smolality+osmolarity 0smometers 'reezing point osmometers "apor presure osmometers

Ion selecti(e electrodes Types of electrodes# principle and function 2eference electrodes 3lass p4 electrodes &recipitate electrodes Solid state electrodes &$0% and &0 electrodes

Electrophoretic techniques Introduction uffers# support materials Endosmosis 'actors affecting the migration rate $linical applications of electrophoresis $hromatographic techniques 3eneral principles &hysical basis of separation Adsorption 7liquid8 solid9 &artion 7liquid8 liquid9 3el filtration 7steric e;clusion9 Ion e;change chromatography Thin layer chromatography 4igh performance liquid chromatography 3as chromatography Automated procedures asic concepts# operation# trouble shooting Automatic disponsors 7dilutors9 Types of automated equipment $ontinuos flow analyzers <iscrete analysers Single channel instruments <iscontinious operation 7 atch processing# single specimen9 =ultichannel instruments Small instruments>.si; channels *arge instruments>non discretory# discretory $ontrifugal analysers

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