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David Barker Carbon dioxide is also a limiting factor in photosynthesis. Carbon dioxide is one of the rarest gasses present in air, and is often a factor that limits the rate of photosynthesis under normal conditions. The optimum concentration of carbon dioxide for photosynthesis is 0.1% and growers of some greenhouse crops such as tomatoes, enrich the air with more carbon dioxide to produce higher yields. CO2 affects enzyme activity, in particular the enzyme that catalyse that catalyses the combination of Ribulose Bisphosphate with carbon dioxide in the lightdependent reaction.