043_12_KM DNA Repair Poster:La out 1 27/02/2012 07:26 Page 1

DNA Repair Pathways

Homologous Recombination
Ionizing radiation Genotoxic chemicals Free radicals Mechanical stress

Mismatch Repair

Nucleotide Excision Repair

UV DNA crosslinkers
FA100 FANCB FANCL FANCM FANCA FANCG

DNA Strand Crosslink Repair

DNA crosslinkers, Cisplatin Mitomycin C, Fork stalling agents
ATR ATRIP FA100 FAAP24 FANCC FANCF FANCE FANCA FANCB FANCL FANCM FANCG FANCF FANCE
P P

FAAP24 FANCC ATRIP ATR

Stalled replication fork

Msh2 Msh6 (MutSa)

Msh2 Msh3 (MutSb)

Global genome repair

Transcription coupled repair

Chk1

Translocation to fork stall site

XPC

HR23B

UBE2T E2 2T E1 Ligase

ATM
P

ATM

ATM

Mre11 Rad50 Nbs1

XPE SMC1
P

-H2AX

Single base pair mismatch

P

1 base pair insertion or deletion

XPC

Transcription block
CSA
RNA Pol I/II

Ubq
P

Ubq q
P

PARP1

Ubq

FANCD2

FANCI

H2A

-H2AX

HR23B

FANCD2/I
P P P P P

histones
CSB
P P
P P P P P

REV1/3

Ubq

Bard1 BRCA1
P

Poly ubiquitin on H2A

Rap80

P P P

Mre11 Rad50 Nbs1

ATM

Histones

Rad51 monomers
P

PCNA

Mdc1
P P P

BRCA2

Abraxas
P P

Single base pair mismatch on leading strand

Pola
ATM MRN complex

Pole Pold

Single base pair mismatch on lagging strand

3 5
Pol/

-H2AX

BRCC36

Rnf8 Ubc13 Caesin Kinase2

Repaired by NER

TFIID XPG XPD XPB TFB5

ERCC1 5 3 3 5
Pol/ Pol/ REV1/3

Resection
3 Rad51BCD XRCC2/3 5 Rad52 MRN complex ATM CtIP 3 CtIP 5

Msh2 Msh6 (MutSa)

RPA XPA

XPF

Translesion bypass by Rev1/3

3 5

Resection

Pole Pola Pold Pola Pold

Pole

XPC

HR23B

3 5 Repaired by HR 3

Rad54

ERCC1 XPG Rad51BCD XRCC2/3 BRCA1

P

XPF TFIID XPD XPB RPA RP PA TFB5 XPA

Rad52

BRCA2 BR

3 5 3 Homology search and D-loop formation

BRCA2
Rad52

3 5

RFC/PCNA and endonuclease activity of MutLa allow Exo1 to resect DNA 3 of the mismatch

5 Pold/e P Pola 3 Exo1 Exo1 5

MutLa endonuclease activity and Exo1 remove mismatch by 5 to 3 resection

Nonhomologous End-Joining
Ionizing radiation Genotoxic chemicals Free radicals Mechanical stress

Mlh1 Pms2 (MutLa)

3
P

BRCA1 Rad51BCD XRCC2/3

RFC

Pold/e

and
P P

ATM

ATM
P

ATM

Mre11 Rad50 Nbs1

g-H2AX
P

RPA

H4K20Me2

5
Rad54

5 3

DNA Pol

DNA Pol

and

PCNA

PCNA
DNA Pol 5 3
DNA Ligase

Homologous recombinations

53BP1

Histones

ATM
P

Mre11 Rad50 Nbs1

Histones Strand tethering

Ku / Lig4 dependent pathway

Ku / Lig4 independent pathway

Pole

DNA synthesis

Pola Pold
Ku70 Ku70 Ku80 Ku80

Rag 1/2 PARP1 PARP1

Replication Resumes
P

DNA PKcs

V Hairpin DNA ends

Homologous chromosome

5
DNA Pol

Microhomology mediated strand annealing

Ku70 Ku70 Ku80 Ku80 DNA PKcs

DNA Pol

Featured DNA Repair antibodies

DAPI

Strand opening and processing by exonuclease activity of WRN

DNA PKcs Ku70 Ku70 Ku80 WRN WRN Ku80 DNA PKcs Artemis
P

Fen1

PNKP

Ligation and formation of Holliday Junction

Non-crossover (cut both junctions vertically or horizontally)

DNA Ligase1

BrdU
Holliday junction resolvase

Anti-BrdU Proliferation Marker antibody (ab8152)

Hela cells stained with ab8152 at 1/100 dilution.

Trial-sized aliquots of your favorite antibodies

Optimize before you commit. DNA damage response Histone modifications RNA polymerase II For more details visit: www.abcam.com/panels

gamma H2A.X (phospho S139) antibody (ab2893)

Pachytene spermatocytes stained with ab2893 at 1/200.

Flap excision

Polb Hairpin DNA cut by Artemis

DNA ligation

XRCC4

DNA Ligase 4 XLF

XRCC1

DNA Ligase 3

Crossover (cut both black or both red arrows)

Merge

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Copyright 2012 Abcam, All Rights Reserved. The Abcam logo is a registered trademark. All information / detail is correct at time of going to print. Produced in collaboration with James Haber and Farokh Dotiwala, Brandeis University.

043_12_KM

No deletion Accurate repair

Short deletion Inaccurate repair