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Available online at www.sciencedirect.com

Complementary Therapies in Medicine (2013) xxx, xxxxxx

ScienceDirect
journal homepage: www.elsevierhealth.com/journals/ctim

Effect of Spiritist passe (Spiritual healing) on growth of bacterial cultures

Giancarlo Lucchetti a,b,c,d,, Renata Ferreira de Oliveira b, alves b, Juliane Piasseschi de Bernardin Gonc Suely Mitoi Ykko Ueda c, Lycia Mara Jenne Mimica c, Alessandra Lamas Granero Lucchetti b,d
a

Federal University of Juiz de Fora, Brazil So Paulo Medical Spiritist Association, Brazil c Santa Casa of So Paulo, Brazil d Joo Evangelista Hospital, Brazil
b

KEYWORDS
Bioeld therapies; Spiritism; Passe; Bacteria; Therapeutic touch

Summary Background: Bioeld therapies are approaches that harness energy elds to inuence the human body. These therapies encompass Reiki, Qigong, Therapeutic Touch, Johrei and Spiritist passe, among others. The aim of this study was to evaluate bacterial growth in two groups of cultures subjected to bioeld therapy (Spiritist passe and laying on of hands (LOH)) in four situations (no intention, intention to inhibit bacterial growth, intention to promote growth, and inuence of a negative factor) and compare them with a no LOH/no treatment group. Methods: Bacterial cultures (Escherichia coli ATCC) were randomized and allocated into three groups: Spiritist passe, LOH, and no LOH. Bacterial growth was assessed using the McFarland Nephelometer Scale. A One-way ANOVA was performed to determine group differences in bacterial growth at 48 h, and at 1 week after each situation. Results: A total of 11 Spiritist passe healers, 10 LOH laymen and no LOH tubes were assessed. Under the intention to inhibit bacterial growth condition, statistically signicant differences were found between the Spiritist passe and no LOH Groups (p = 0.002 after 48 h, and p = 0.008 after one week) and also between the Spiritist passe and LOH Groups (p = 0.005 after 48 h, and p = 0.009 after one week). No statistically signicant difference was detected for the other situations tested (no intention, intention to promote growth and inuence of a negative factor). Conclusions: We concluded that Spiritist passe effectively inhibited growth in bacterial cultures compared to LOH with intention or no LOH. Further studies comparing different intentions and types of LOH in cultures of cells and microorganisms are warranted. 2013 Elsevier Ltd. All rights reserved.

Corresponding author at: Av. Juriti, 367 apto 131, Moema, So Paulo, SP, Brazil. Tel.: +55 11 981199001. E-mail addresses: g.lucchetti@yahoo.com.br, gian.tln@terra.com.br (G. Lucchetti).

0965-2299/$ see front matter 2013 Elsevier Ltd. All rights reserved. http://dx.doi.org/10.1016/j.ctim.2013.08.012

Please cite this article in press as: Lucchetti G, et al. Effect of Spiritist passe (Spiritual healing) on growth of bacterial cultures. Complement Ther Med (2013), http://dx.doi.org/10.1016/j.ctim.2013.08.012

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G. Lucchetti et al. inuence of a negative factor) and compare them with a no LOH/no treatment group.

Introduction
Complementary medicine has grown signicantly in recent decades. An estimated 40% of patients in the United State use some type of complementary therapy1 where at least 13% use spiritual healing or prayer, and 1% energy therapies.2 Bioeld modalities3 are dened as those therapies intended to affect energy elds that purportedly surround and interpenetrate the human body. These therapies involve touch or placement of the hands in or through bioelds, the existence of which have not yet been scientically proven. These therapies include Reiki, Qigong, Therapeutic Touch, Johrei and Spiritist passe, among others. This latter approach numbers among the therapies employed by Spiritism, the third most prominent religion in Brazil that adopts a dualistic concept of human beings. It postulates that we are essentially immortal spirits that temporarily inhabit physical bodies for several necessary incarnations to attain moral and intellectual improvement. It also implies a possible benecent or malecent inuence of the spirits over incarnate humans.4 With Spiritism emerged Complementary Spiritist Therapy,5 based on a range of therapeutic resources including prayer, uidotherapy (uidic or magnetized water), charity, spirit education, disobsession (spirit release therapy) and the Spiritist passe (a kind of laying on of hands (LOH) which, according to Spiritism, results in an exchange of uids and energies derived from the healer, from good spirits or a combination of both). Spiritist therapy, although not yet fully understood or extensively studied, has shown promising indirect results such as those reported in a recent systematic review.6 Several studies involving bioeld therapies have shown positive results, in some cases showing differences between LOH with intention versus the same approach without a dened intention.6 However, in the last decades, numerous systematic reviews in this eld showed controversial results and heterogeneous evidence.79 Other studies have shown the effect of bioeld therapies on human cells and bacteria.10,11 It is well established that several physical factors can inuence bacterial growth such as temperature, pH and salinity.12 Nevertheless, few studies have been conducted aimed at elucidating the relationship between behavioral factors and bacterial growth. In 2006, a study assessing the inuence of Reiki in bacterial cultures was published. According to the study results, no overall difference was found between the Reiki and control plates in the non-healing context. In the healing context however, the Reiki treated cultures exhibited signicantly more bacteria overall than did controls.11 Nevertheless, one of the current knowledge gaps in this eld of research is whether differences exist between two different types of LOH with intention (a therapy deemed spiritual versus a non-spiritual therapy). Similarly, it is not clear if different intentions such as that of promoting or inhibiting growth can in fact positively or negatively inuence the proliferation of bacterial cultures. Against this background, the aim of this study was to evaluate bacterial growth in two groups of cultures subjected to bioeld therapy (Spiritist passe and laying on of hands (LOH)) in four situations (no intention, intention to inhibit bacterial growth, intention to promote growth, and

Methods
An experimental study was conducted at the Microbiology Laboratory of the Medical Sciences Faculty of the Santa Casa de Misericrdia of So Paulo between April and June 2011. This laboratory is one of the references for microbiological analysis in So Paulo, Brazil. It has a controlled environment and it follows the procedures from the Manual of Clinical Microbiology12 which sets the standard for state-of-the-science laboratory practice. The main hypothesis was that bacterial growth in cultures submitted to different types of LOH varies according to the type of intention and faith of the participants. Three different groups were employed to test this theory: Spiritist passe (spiritual healing) group: according to Spiritism,6 passe is dened as a transfusion of energy which changes the cellular eld and a transfusion of psychic energy. Spiritist healers undergo a course of three years in which they learn the fundamentals of the application of passes with the aim of curing individuals through practical classes. Having completed this course they are authorized to act in the capacity of healers within Spiritist Centers. For the present study, healers afliated to the Spiritist Federation of So Paulo State (one of the most renowned Spiritist institutions in Brazil) were recruited. Inclusion criteria for healers was having at least 2 years of experience, aged older than 18 years, and having taken part in courses accredited by the institution. Laying on of hands (LOH) laymen group: the group was randomly selected from among groups of friends of relatives of the researchers, staff or trainees at the Santa Casa de So Paulo Hospital. Inclusion criteria for this group was adults (older than 18 years of age), not belonging to the Spiritist religion, and no prior knowledge of LOH techniques such as Reiki, Johrei or Therapeutic Touch. No LOH/No treatment group: Analysis of bacterial culture growth without LOH, in other words by natural course.

Experimental procedures
Bacterial cultures were randomized and allocated into the three groups: Spiritist passes, LOH, and no LOH. The tubes were numbered consecutively and a computerized random-number generator was used to create a random sequence. Then, the rst tube in the random sequence was included in the Spiritist passe group, the second tube was included in the LOH group, the third tube was included in the No LOH group and so on. The bacterial samples were placed in identical tubes with culture medium and presented uncovered in metal test tube racks to the volunteers. A notebook device with headphones was used to enable participants to watch and listen to the videos (see below). The Spiritist healers were allowed to previously prepare the setting through 5 (ve) min of prayer and relaxation. The passe or LOH was carried out continuously for 10 min (witnessed by two research assistants) at a distance of 1015 cm from the tubes containing the bacterial cultures

Please cite this article in press as: Lucchetti G, et al. Effect of Spiritist passe (Spiritual healing) on growth of bacterial cultures. Complement Ther Med (2013), http://dx.doi.org/10.1016/j.ctim.2013.08.012

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3 numbered from 0.5 to 10, containing different amounts of barium chloride and sulfuric acid. The different concentrations of barium chloride and sulfuric acid determine the bacterial counts. Thus, tube number 0.5 corresponds to 150 million bacteria, tube number 51.5 billion, and tube number 103 billion bacteria, per mL of medium. To increase reliability of the results, tubes were withdrawn randomly (precluding identication) and analyses of growth were performed in triplicate by three independent examiners. In the event of no consensus, examiners convened to reach a decision on the nal result. Results analysis was carried out as per manufacturers instructions (Probac do Brasil) by comparing tubes containing NefelobacTM against those containing bacterial culture, side by side with the naked eye. The tubes used to hold NefelobacTM and bacterial culture were homogenized to produce a uniform suspension (turbidity). Printed texts and a Wickerham card15 were placed to carry out comparative readings of the tubes, where lesser or greater clarity of the letters and lines seen through the tubes indicated higher and lower turbidity, respectively. The outcome assessment took place by an assessor blind to treatment allocation. After the analysis at one week, cultures were seeded onto blood Agar medium to test the viability of the strains. Bacterial samples not viable after one week were excluded from the study (7 tubes from the LOH group were excluded).

Spiritist passe (spiritual healing) on growth of bacterial cultures (this distance was chosen because the Spiritist healers are trained to apply the passe within this distance). The experimental sessions were performed between 8 a.m. and 3 p.m. for three consecutive days. Participants were submitted to a well-being scale (10 cmlong line, numbered from zero to ten), containing questions on general well-being, peace, happiness, spiritual wellbeing, nervousness and irritability. Both groups applied passe or LOH with lights out (to increase concentration) for 10 min and under four different conditions: (1) LOH with no intention for 10 min: in this experiment the participants (Spiritist passe and LOH) watched a video about the industrial production of ethanol fuel, describing the process of obtaining, storing and manufacturing the product (in order to distract their attention). Healers were instructed to place their hands over the cultures while paying attention to the video with no intention requested. (2) LOH with the intention of inhibiting bacterial growth for 10 min: participants were instructed to kill the bacteria (prevent growth) since they were the cause of infection and were harmful to humans. At this point, participants watched a video of aspects of nature and relaxing sounds with no religious connotation. (3) LOH with the intention of promoting growth: participants were instructed to promote growth of the cells. At this point, participants watched the same video of aspects of nature and relaxing sounds with no religious connotation. (4) LOH with a negative impact: In this experiment, participants watched the rst 10 min of the lm Saving Private Ryan showing scenes of violent combat on Dday during the Second World War. Participants were instructed to place their hands over the cultures while paying attention to the video with no intention requested.

Statistical analysis
The data collected were input to the SPSS 17.0 (SPSS Inc.) statistics package. Demographic data for the groups was compared using the t test (continuous variables) and Chisquare test (categorical variables). A one-way ANOVA was performed to determine group differences (Passe, LOH and no LOH) in bacterial growth/counts at 48 h, and at 1 week after each situation. Then, a TukeyKramer post hoc analysis was performed. To increase homogeneity and to approach normality of the data, the variables used in the analysis were transformed to Z-scores before analysis. A p value <0.05 was considered statistically signicant and the condence interval was set at 95%. All statistical analyses were conducted under blind circumstances by an independent statistician.

Bacterial culture
The agent Escherichia coli ATCC was used in all experiments, based on a previous study which investigated the effects of Reiki treatments on E. coli bacteria.11 The agent was chosen inoculated into screw-capped tubes containing aliquots of 5 mL of TSB (Tryptic Soy Broth; Probac do BrasilTM ) medium where density corresponding to the 0.5 standard on the McFarland scale. For each condition investigated, it was decided to use 3 tubes in a bid to improve reliability and increase the sample count. Cultures were stored in an incubator at 37 C. Inoculums were assessed at 48 h and again at one week, post experimentally. The McFarland Nephelometer Scale (NefelobacTM , Probac do Brasil),13 was used to determine the intensity of multiplication in liquid culture medium. This multiplication manifests in liquid media via an increase in particles (bacteria) which in turn block the free passage of light, causing turbidity or opacity of the medium. The higher the bacteria count, the greater the opacity of the culture medium. NefelobacTM was prepared according to the nephelometric McFarland standard formula.12,14 Briey, this consists of a series of 11 tubes

Ethical issues
The study was evaluated and approved by the Santa Casa of So Paulo Institutional Review Board and all participants signed a consent form.

Results
Characteristics of participants
A total of 12 Spiritist healers and 12 individuals for the LOH group was selected. Due to personal problems, one of the Spiritist healers and two from the LOH group were unable to

Please cite this article in press as: Lucchetti G, et al. Effect of Spiritist passe (Spiritual healing) on growth of bacterial cultures. Complement Ther Med (2013), http://dx.doi.org/10.1016/j.ctim.2013.08.012

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4 take part, giving a nal sample of 11 and 10 participants in the Spiritist passe and LOH groups, respectively. Three tubes per participant were used for each experimental condition, i.e. each participant performed laying on of hands on three tubes at a time (3 tubes with no intention, 3 tubes inhibiting growth, 3 tubes promoting growth and 3 tubes with negative inuence). Thus, a total of 132 tubes were used for the 11 Spiritist healers (33 per condition), 113 for the 10 subjects in the LOH group (28.2 per situation excluding 7 tubes due to non-viability of the sample after one week) plus 33 tubes to serve as no LOH group. The mean age of subjects in the Spiritist healer group was 51.8 (SD: 10.8) years, with subjects being predominantly females (72.7%) with a mean experience of 16.2 (SD: 11.4) years applying passes at Spiritist centers. The mean age of subjects in the LOH group (non-Spiritist healers) was 35.1 (SD: 16.9) years (p = 0.016 compared to Spiritist healers) and subjects were predominantly females (80.0%), with no signicant difference to the Spiritist passes group in terms of gender (p = 0.55).

Bacterial growth after 48 h

The assumptions for performing the ANOVA parametric test were met. Z-scores were used for dependent variables (bacterial growth/counts in each situation: no intention, increase, decrease and negative) in order to reduce the effect of non-normality of this variable. On the analysis of bacterial growth after 48 h (Graph 1), the one-way ANOVA test showed differences for the inhibition of bacterial growth condition (F = 7.932, p = 0.001, eta squared: 0.148) but not for the other situations: promoting bacterial growth (F = 2.015, p = 0.139), no intention (F = 2.093, p = 0.129) and negative impact (F = 2.467, p = 0.091). The TukeyKramer post hoc test was used given the lack of homogeneity among the groups. For the inhibition of bacterial growth situation, statistically signicant differences were found between the no LOH and the passe groups (p = 0.002) and likewise between the LOH and the passe groups (p = 0.005).

Bacterial growth after one week General well-being of participants

A difference in participants well-being was noted among the groups. Mean physical well-being of the Spiritist passe and LOH groups was 8.82 vs. 7.30 (p = 0.006), 9.36 vs. 7.20 (p = 0.017) for inner peace, 8.73 vs. 7.20 (p = 0.151) for happiness, 9.36 vs. 7.70 (p = 0.111) for spiritual well-being, 0.45 vs. 3.90 (p = 0.000) for nerves, 0.55 vs. 3.80 (p = 0.011) for irritability, and 2.55 vs. 4.50 (p = 0.0155) for worries. No statistically signicant difference was found (p = 0.256) for response to the question on whether subjects had experienced a bad time during the week. The analysis of bacterial growth after one week can be seen in Graph 1. The one-way ANOVA test showed differences for the inhibition of bacterial growth situation (F = 6.210, p = 0.003, eta squared: 0.118) but not for the other situations: promoting bacterial growth (F = 0.090, p = 0.914), no intention (F = 0.578, p = 0.563) and negative impact (F = 2.080, p = 0.131). The TukeyKramer post hoc test was used given the lack of homogeneity among the groups. For the decrease in bacterial growth situation, statistically signicant differences were found between the no LOH and the passe groups (p = 0.008) groups and likewise between the LOH and the passe groups (p = 0.009) groups.

Graph 1

Bacterial growth 48 h, and one week, after experiment by group (mean, SE).

Please cite this article in press as: Lucchetti G, et al. Effect of Spiritist passe (Spiritual healing) on growth of bacterial cultures. Complement Ther Med (2013), http://dx.doi.org/10.1016/j.ctim.2013.08.012

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5 authors, should be assessed by this type of study21 and, (c) the use of nonhuman populations (such as animals or bacteria) which, could solve some methodological problems in such research.7 However, our results should also be interpreted with caution. Firstly, this was an experimental study in which it was not possible to determine whether the inuence seen in bacterial growth also occurs in vivo. Secondly, a clear difference in age and well-being between participants from the Spiritist passe and LOH groups was observed, pointing to a possible role of other factors inuencing these results. Thirdly, a larger sample may yield other statistical differences not observed in the present study. Fourthly, the results were obtained from only one center. A future multiple center trial is warranted in order to enhance the validation of our results. Comparing to other studies, we found some remarkable differences. For instance, Rubik et al.11 found that Reiki was capable of promoting bacterial growth. On the other hand, our study found that the Spiritist passe was capable of inhibiting bacterial growth. This apparent contradiction could be explained by the intentions involved in both studies. In the rst study, healers were asked to help the bacteria recover and grow. In the latter, healers were instructed to inhibit growth. Interestingly, when Spiritist healers were asked to promote growth, they failed to replicate Rubik ndings. This nding, not yet fully understood, leads us to speculate that Spiritist healers motivation and concentration may have been greater when asked to kill bacteria harmful to humans and the cause of diseases than under other conditions. Moreover, when Spiritist healers have no dened intention, bacteria were found to exhibit similar growth to control samples. This result lends further weight to the hypothesis that intention to inhibit growth was able to inuence bacteria, as shown in Graph 1. Although, at present, there are only very tenuous theoretical foundations underlying the mechanism responsible for healing, authors believe that an energy (undetected by normal scientic methods) is activated during the process of healing and transferred to the patient.22 In Spiritism,6 laying on of hands is dened as an energy transfusion, changing the cellular eld and a psychic energy transfusion, denoting the inuence of the spiritual energy on physical health. According to Hodges and Scoeld,22 the purpose of the healer is to channel the energy from what is usually termed a higher source into the patient where, rstly, it increases the energy levels and ows, and re-establishes a balance; secondly, it activates the natural healing force within patients, helping them to heal themselves and to return toward a state of normality. Our ndings have also some implications for clinicians. The role of spiritual healing on the growth of bacterial cultures in two studies (the present one and Rubik et al. [11]) could foster the discussion on whether these therapies could have the same impact in clinical samples such as patients with sepsis or treated with antibiotics. In addition, although spiritual treatments are frequently used by patients, there is little evidence available. Increasing evidence regarding these issues could help health professionals in recommending or not these practices to their patients.

Spiritist passe (spiritual healing) on growth of bacterial cultures

Discussion
In the present study, a difference was noted between the Spiritist healer group and the other groups for inhibiting growth in bacterial cultures. These results were maintained even one week after the passe intervention. The possibility of humans exerting change in infectious agents is a subject little studied in the medical literature, with few reports available on the theme. The present study revisits the discussion as to whether humans are capable of not only inuencing their own organism but inuencing other cell types such as bacteria and broblasts. In addition, these data raise the possibility of there being a difference between only having good or bad intentions in LOH and actually making a spiritual connection. This theory is supported by the fact that, for all of the situations studied, the LOH group showed no signicant improvement on results found in the Control group, suggesting that intention alone was unable to impact bacteria in the experiment. Given that all bioeld therapies (Reiki, Qigong, Therapeutic Touch, Johrei and Spiritist passe) involve some kind of ritual or systematization, it could be inferred that this element shall be a prerequisite for the approach to effectively inuence outcomes. The inuence of bioeld therapies on the growth of different cell types has been a constant focus of research and reports in the medical literature.16 Studies based on therapeutic touch have conrmed increased proliferation of human cells in cultures,10 DNA synthesis and mineralization of human osteoblasts17 as well as increased blood hemoglobin and hematocrit level.18 However, studies in bacterial cultures remain scarce in the literature. In a recent study, Rubik et al.11 assessed the inuence of Reiki on bacterial cultures and reported no overall difference was found between the Reiki and control plates in the non-healing context. In the healing context, the Reiki treated cultures overall exhibited signicantly more bacteria than control. Some studies conducted in China19 have shown that external qi emitted from the hands of qigong masters can either increase or decrease counts of bacteria, normal and cancer cells, demonstrating two forms of external qi: positive qi, with benecial effects; and negative qi, with deleterious effects on cells. Recently, Morse and Beem20 reported a 54-year-old severely ill man who had hepatitis C who was treated with experimental high-dose interferon/ribavirin therapy with resultant profound anemia and neutropenia. Spiritist passe and Reiki were used for complementary treatment and he found statistically signicant relationships between these therapies, a quieting of the electronically created white noise of the RNG during healing sessions, and improvement in the patients absolute neutrophil count. Nevertheless, to date, no experimental studies have assessed Spiritist passe as a modality in bioeld therapies. Noteworthy, the present study presents some strengths that should be mentioned: (a) the investigation of several different intentions and the comparison between healers and laymen, weakening the possibility that these results could be justied by the imposition of hands alone; (b) the measurement of some psychological aspects of participants through well-being scales which, in accordance with some

Please cite this article in press as: Lucchetti G, et al. Effect of Spiritist passe (Spiritual healing) on growth of bacterial cultures. Complement Ther Med (2013), http://dx.doi.org/10.1016/j.ctim.2013.08.012

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US adults: 19972002. Altern Ther Health Med 2005;11: 429. Ni H, Simile C, Hardy AM. Utilization of complementary and alternative medicine by United States adults: results from the 1999 national health interview survey. Med Care 2002;40:3538. Miles P, True G. Reikireview of a bioeld therapy history, theory, practice, and research. Altern Ther Health Med 2003;9:6272. Moreira-Almeida A, Silva de Almeida AA, Neto FL. History of Spiritist madness in Brazil. Hist Psychiatry 2005;16: 525. Lucchetti G, Aguiar PRDC, Braghetta CC, Vallada CP, MoreiraAlmeida A, Vallada H. Spiritist psychiatric hospitals in Brazil: integration of conventional psychiatric treatment and spiritual complementary therapy. Cult Med Psychiatry 2011;36: 12435. Lucchetti G, Lucchetti AL, Bassi RM, Nobre MR. Complementary spiritist therapy: systematic review of scientic evidence. Evid Based Complement Alternat Med 2011;2011:835945. Astin JA, Harkness E, Ernst E. The efcacy of Distant Healing a systematic review of randomized trials. Ann Intern Med 2000;132:90310. Lee M, Pittler M, Ernst E. Effects of reiki in clinical practice: a systematic review of randomised clinical trials. Int J Clin Pract 2008;62:94754. Abbot NC. Healing as a therapy for human disease: a systematic review. J Altern Complement Med 2000;6:15969. Gronowicz GA, Jhaveri A, Clarke LW, Aronow MS, Smith TH. Therapeutic touch stimulates the proliferation of human cells in culture. J Altern Complement Med 2008;14:2339. Rubik B, Brooks AJ, Schwartz GE. In vitro effect of Reiki treatment on bacterial cultures: role of experimental context and practitioner well-being. J Altern Complement Med 2006;12:713. Versalovic J. Manual of clinical microbiology. Washington, DC: ASM Press; 2011. Nefelobac ESCALA NEFELOMTRICA DE MC FARLAND. http://www.probac.com.br/bulas/nefelobac.pdf Lennette EH, Ballows A, Hausler W. Manual of clinical microbiology. Washington, DC: American Society of Microbiology; 1985. Wickerham LJ. Taxonomy of yeasts. Washington, DC: US Dept. of Agriculture; 1951. Jain S, Mills PJ. Bioeld therapies: helpful or full of hype? A best evidence synthesis. Int J Behav Med 2010;17:116. Jhaveri A, Walsh SJ, Wang Y, McCarthy M, Gronowicz G. Therapeutic touch affects DNA synthesis and mineralization of human osteoblasts in culture. J Orthop Res 2008;26: 15416. Movaffaghi Z, Hasanpoor M, Farsi M, Hooshmand P, Abrishami F. Effects of therapeutic touch on blood hemoglobin and hematocrit level. J Holist Nurs 2006;24:418. Sancier KM, Hu B. Medical applications of qigong and emitted qi on humans, animals, cell cultures, and plants: review of selected scientic research. Am J Acupunct 1991;19: 36777. Morse ML, Beem LW. Benets of Reiki therapy for a severely neutropenic patient with associated inuences on a true random number generator. J Altern Complement Med 2011;17:118190. Targ E. Evaluating distant healing: a research review. Altern Ther Health Med 1997;3:748. Hodges R, Scoeld A. Is spiritual healing a valid and effective therapy? J R Soc Med 1995;88:2037.

6 In our opinion, future studies should focus on the differences among techniques such as therapeutic touch, Spiritist passe, Johrei and Reiki in clinical outcomes. Another aspect that should be more evaluated is the comparison of intentional laying on of hands and healing techniques based on a high healing power, such as attempted in the present study. Furthermore, another future direction is focusing on the identication of which types of energies were irradiated from the imposition of hands. We conclude that LOH by Spiritist passe was able to effectively inhibit the growth of bacterial cultures compared to LOH with intention and no laying on of hands. Further studies comparing different intentions and types of laying on of hands in cultures of cells, other microorganisms and other bacterial species are warranted.

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Authors contributions
GL has made substantial contributions to conception and design, acquisition of data, and analysis and interpretation of data; has been involved in drafting the manuscript; and has given nal approval of the version to be published. RFO has made substantial contributions to acquisition of data; has been involved in revising the manuscript critically for important intellectual content; and has given nal approval of the version to be published. JPBG has made substantial contributions to acquisition of data; has been involved in revising the manuscript critically for important intellectual content; and has given nal approval of the version to be published. SMYU has made substantial contributions to conception and design; has been involved in revising the manuscript critically for important intellectual content; and has given nal approval of the version to be published. LMJM has made substantial contributions to conception and design; has been involved in revising the manuscript critically for important intellectual content; and has given nal approval of the version to be published. ALGL has made substantial contributions to conception and design, analysis and interpretation of data; has been involved in revising the manuscript critically for important intellectual content; and has given nal approval of the version to be published.

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Conict of interest statement

The authors declare that there is no conict of interest.

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Acknowledgement
The authors would like to extend their thanks to the volunteers who took part in this study.
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References
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21. 22.

Please cite this article in press as: Lucchetti G, et al. Effect of Spiritist passe (Spiritual healing) on growth of bacterial cultures. Complement Ther Med (2013), http://dx.doi.org/10.1016/j.ctim.2013.08.012