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ASSIGNMENT ON
RENNET PRODUCTION
BY
S.SHANMUGA PRIYA
BTF-07-023
Cottage cheese
Roquefort
Stilton
Blue cheese
Gorgonzola
Camembert
Brie
Swiss
Baby Swiss
Gouda
Edam
Limburger
Cheddar
Colby
Sweet Brick
Muenster
Havarti
Mozzarella
Provolone
Parmesan
Romano
MICROBIAL RENNET:
MICROBIAL RENNET:
RECOMBINANT RENNET:
Gene for calf chymosin was one of the first genes for mammalian enzymes
that was cloned and expressed in microorganisms. The use of Chymosin
(genetically-engineered rennin or rennet) for cheese-making was the first
commercial application of food biotech (1988). In the 1960s the Food and
Agriculture Organization of the United Nations predicted a severe shortage of
calf rennet. It was anticipated that an increased demand for meat would lead
to more calves being reared to maturity, and hence less rennet would be
available. Over the last 30 years several substitutes for calf rennet have
been developed, allowing the supply of enzymes to keep pace with cheese
production. The first Chymosins in the early 1980s were derived from
genetically-modified microbes (Escherichia coli, Kluyveromyces lactis and
Aspergillus niger). Numerous groups have since followed their lead, using
other microbes, so that chymosin has now been obtained from food yeasts.
Today about 90% of the hard cheese is made using chymosin from genetically-
modified microbes and 60% of U.S. hard cheese was made with genetically
engineered chymosin it has up to 80% of the global market share for rennet..
Chymosin is identical to the enzyme obtained from animals. This can be used
to produce better quality cheese than the fungal or other animal (non-calf)
rennets. These bioengineered enzymes behave in exactly the same way as calf
rennin, but their activity is more predictable and they have fewer
impurities. Such enzymes have gained the support of vegetarian organizations
and of some religious authorities. Chymosin obtained from recombinant
organisms has been subjected to rigorous tests to ensure its purity. Highly
recommended by some of the best known cheese makers this is high quality
rennet, originating from animal sources, but containing no animal products
itself a vegetable product with animal origins. the most widely used
genetically engineered rennet is produced by the fungus Aspergillus niger.
Other micro organisms are Escherichia coli, Kluyveromyces lactis.
Escherichia coli . It is not regarded as GRAS (Generally Regarded As Safe)
because recombinant proteins are frequently synthesized as intracellular
inclusion bodies process cost increases. Cheese production with chymosin is
similar to production with natural calf rennet. Preparation of calf rennet
has two forms of chymosin A in proportion 40% & chymosin B 60 % Genetic
rennet contains only one of the known main chymosin types, either type A or
type B and Other do not exist. Genetically engineered chymosin and natural
pepsin is mixed to imitate the complexity of natural rennet and to get the
same results in coagulation and in development of flavor and taste. Such
rennets are suitable for vegetarians if there was no animal based
alimentation used during the production in the fermenter.
Aspergillus niger
Aspergillus niger is a fungus and one of the most common species of the
genus Aspergillus. It is a common contaminant of food. A. niger is cultured
for the industrial production of many substances. Various strains of A. niger
are used in the industrial preparation of citric acid (E330) and gluconoic
acid (E574). A. niger fermentation is "generally regarded as safe“ by the
FDA.
CONTAINED USE:
EU Contained Use Directive (Art. 2c): ‘contained use’ shall mean any
activity in which micro-organisms are genetically modified or in which such
GMMs are cultured, stored, transported, destroyed, disposed of or used in any
other way, and for which specific containment measures are used to limit
their contact with, and to provide a high level of safety for, the general
population and the environment;
FERMENTATION:
The production organism is grown in several stages to build up the
inoculum for large-scale production. After inoculation to the fermenter, the
cells are grown aerobically under the proper conditions of pH, temperature,
nutrient composition, etc. After chymosin reaches a desired level in the
fermentation broth, the fermentation is stopped and the fungal cells are
inactivated and separated from the liquid.