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Organization
OVERVIEW. Begin with a brief overview that reveals the object's (a) overall framework, arrangement, or shape, and (b) purpose or function. PARTS. Divide the object into parts and describe each part (a) in enough detail to use, make, or draw it, and (b) in a way that reveals its role, its relation to other parts. ORDER. Organize the part descriptions to help your reader: (a) spatial order (top to bottom, outside to inside), or (b) priority order (most to least important), or (c) chronological order (order of [dis]assembly).
Content
SPECIFICS. o Include relevant specific features (such as size, shape, color, material, technical names). o Omit irrelevant background, confusing details, and needless words. COMPARISON. Compare features or parts with other things already familiar. CONTRAST. Contrast properties with different ones to reveal their significance.
FORMAT. Clarify your text with: o Heads. Identify topics with clear, nested section headings. o Lists. Itemize related features with indenting and marks. o Figures. Integrate figures and text with labels and references. VERBAL CUES. Guide your reader's expectations with: o Parallelism. Use parallel words and phrases for parallel ideas. o Proleptics. Use verbal links (also, but, however, etc.) to signal how your description fits together.
FEATURE: WHY:
FEATURE: WHY:
FEATURE: WHY:
FEATURE: WHY:
FEATURE: WHY:
FEATURE(S):
third-class lever, with its fulcrum (F) at the welded end. High force applied to its middle by the handle bend (above it) moves the cutting edge gently through enough distance to meet the facing edge below it, carefully cutting any finger nail inserted between the beveled edges.
WHY:
The information in DNA can be used for many purposes. Read the following passage to see how the information can be useful. DNA fingerprinting and DNA typing Southern Blots are not just a research tool. They are widely used in forensic laboratories to identify individuals who have left blood or other DNA- containing tissues at the scenes of crimes. Such DNA typing has its roots in a discovery by Alec Jeffreys and his colleagues in 1985. These workers were investigating a DNA fragment from a gene of a human blood protein, -globin, when they discovered that this fragment contained a sequence of bases repeated several times. This kind of repeated DNA is called minisatellite. More interestingly, they found similar minisatellite sequences in other places in the human genome, again repeated several times. This simple finding turned out to have far-reaching consequences, because individuals differ in the pattern of repeats of the basic sequence. In fact, two individuals have only a remote chance of having exactly the same pattern. That means that these patterns are like fingerprints; indeed they are called DNA fingerprints.
A DNA fingerprint is actually a southern blot. To make one, investigators first cut out the DNA under study with a restriction enzyme such as HaeIII. Jeffreys chose this enzyme because the sequence he had found did not contain a HaeIII recognition site. That means HeaIII will cut on either side of the minisatellite regions, but not inside. In this case, the DNA has three sets of repeated regions, containing four, three, and two repeats, respectively (some regions containing unrelated DNA sequences). Thus, three different size fragments bearing these repeated regions
will be produced.
Next, the fragments are electrophoresed, denatured, and blotted. The blot is then probed with a labeled minisatellite DNA, and the labeled bands are detected with X ray film, or by phosphor imaging. In this case, three labeled bands occur, so three dark bands will appear on the film. Real animals have more complex genome than the simple piece of DNA in this example, so they will have many more than three fragments that contain a minisatellite sequence that will react with the probe. As we have already mentioned, this is such a complex pattern of fragments that the patterns for two individuals are extremely unlikely to be identical, unless they come from monozygotic twins. This complexity makes DNA fingerprinting a very powerful identification technique.
3 Generally, individuals differ in the pattern of repeats of the basic sequences in DNA
False True
4 DNA fingerprinting is the most widely used identification technique for criminal identification.
True False
5 Police can identify a criminal from his identical twin using DNA fingerprinting.
True False
6 A restriction enzyme such as HaeIII will cut on inside the minisatellite regions.
True False
Multiple-choice questions
*Three dark bands appear on the film. *The sequence chosen does not contain a Hae III recognition site. *Detected by X-ray film or by phosphor imaging. *Electrophoresed, denatured and blotted. *The DNA has three sets of repeated regions. *Three different size fragment bearing repeated regions are produced. *The blot is probed with a labeled minisatellite DNA.
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Vocabulary
If the molecules are moved through a liquid or gel under the influence of an electric field, then the molecules are .. The complete set of genes present in a cell is called Detection of crime by DNA fingerprinting is done by . scientists. A group of cells making animal or plant body parts or organs is called A .divides DNA molecules at specific sequences of nucleotides.
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Changing the properties of a molecule by disruption of its conformation is called . Twins from a single ovum are called .twins. A ... finding can be widely applicable and carries many consequences.
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