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SWARNAVA ROY, Ph.D.

Present Address: 101 Sovabazar Street, Kolkata, 700005, West Bengal,


India; Cell# 9830067144, Home: 91-033-2554-4955, 91-033-24413569
Previous work Address: Cincinnati Childrens Hospital Medical Center
(CCHMC), 3333 Burnet Avenue, Cincinnati, OHIO, 45229, USA
E-Mail:swarnava.roy@gmail.com, swarnava_roy@yahoo.com
Area of
Research &
Objective

Work
Experience

A researcher with over ten years of research experience,


which includes PhD degree in Biochemistry from Louisiana
State University (LSU) and over five years of post-doctoratal
research experience from the following institutes, NIDDK,
National Institutes of Health (NIH) and Cincinnati Childrens
Hospital Medical Center (CCHMC) working in Translational,
Clinical & Molecular Immunology based research projects.
Project at CCHMC undergoing Clinical Trial (ClinicalTrials.gov
Identifier: NCT01479439). Proposed future research project
focuses in the area of Molecular Immunology, investigating on
Molecular Pathways activated during Kidney Diseases.
Has worked with a wide variety of experimental model systems
encompassing Cancer cell lines, extensive experience
working and handling mice and Drosophila model.
Extremely hardworking, productive, methodical, focused and
goal oriented and has a strong publication record.
Interested in Associate Professor position in areas related to
Molecular Immunology, Hematology, Biochemistry, Molecular
biology, Cancer biology, Cellular biology, developmental
biology, therapeutics and drug discovery.
Working
as
an
Online
Faculty
at
BrainMass.com
(https://brainmass.com/experts/SWARNAVAROY)
and
at
InstaEDU.com (2015-present).
Worked as a Research Fellow at the Cincinnati Childrens
Hospital Medical Center, Cincinnati, Ohio (2011-2014).
Worked as a Post Doctorate Fellow at the National Institute of
Diabetes and Digestive and Kidney Diseases (NIDDK), National
Institutes of Health, Bethesda, Maryland.(2009-2011).

Education

Publications

Earned PhD degree at the Louisiana State University, Baton


Rouge, LA, doing research on areas such as Biochemistry,
Molecular Biology, Cell biology and Genetics (2003-2008).
Worked as a teaching Assistant at LSU, Baton Rouge, LA,
teaching several undergraduate courses (2003-2008).
PhD in Biochemistry, Louisiana State University, Baton Rouge,
LA, USA (2003- 2009).
M.S. in Microbiology, Bangalore University, Bangalore, India
(2001-2003).
B.S. in Microbiology, Bangalore University, Bangalore, India
(1997-2000).
Swarnava Roy1, Jiang Nan and Craig M Hart (2010) Lack of
the Drosophila BEAF insulator proteins alters regulation of
genes in the Antennapedia complex. (Mol Genet Genomics.
2010 Dec 4).
Swarnava Roy1 and Craig M Hart (2010) Targeted gene
replacement by homologous recombination in Drosophila
stimulates production of second-site mutations. Fly (Austin).
2010 Jan; 4(1): 12-7. Epub 2010 Jan 18.
Swarnava Roy1, Matthew Gilbert and Craig M Hart (2007)
Characterization of BEAF mutations isolated by homologous
recombination in Drosophila. Genetics. April 15, 2007.
Swarnava Roy1, Yian Yee Tan and Craig M Hart (2006) A
genetic screen supports a broad role for the drosophila
insulator proteins BEAF32A and BEAF32B in maintaining
patterns of gene expression. Mol Genet Genomics. 2006 Dec
2.
Kerong Shi, Swarnava Roy, Vaishali Parekh and Sunita Agarwal
(2012) Dysregulation of the differentiation factor HLXB9 as a
possible mechanism of pancreatic -cell tumorigenesis
(Molecular Endocrinology, 2012).
Kyung Hee Chang, Ramesh Nayak, Swarnava Roy, Ajay
Perumbeti, Ashley Ficker, Katie Bezold, Megan Pirman, Susan
Dunn, Xuan Zhou, Yi Zheng, Punam Malik, Jose A. Cancelas
(2014) Vasculopathy associated hyperangiotensinemia
mobilizes hematopoietic stem cells/progenitors through
endothelial AT2R and cytoskeletal dysregulation. Nature
Communications. 2014 (Nat Commun. 2015 Jan 9).

Pending
Publications

Swarnava Roy1, Shiva Kumar Shanmukhappa, Diamantis G.


Konstantinidis, Kyung Hee Chang, Tilat Rizvi, Anastacia Loberg,
Nambirajan Sundaram, Michael Wourms, Bruce Aronow,
Nancy Ratner, David Witte, Theodosia A. Kalfa, Jose A.
Cancelas, and Punam Malik (2014). Increased Oxidant Stress
Activates the Renin-Angiotensin-System to Mediate and
Perpetuate Sickle Nephropathy. Manuscript ready for resubmission to Nature Medicine. Poster for this work presented
at the 55th American Society of Hematology (ASH) Annual
Meeting in New Orleans, LA, December 7-10, 2013.

Molecular
Immunology,
clinical and basic
research based
technical research
skills/experience

Molecular Immunology techniques and protein-protein


interaction/protein nucleic acid interaction techniques:
a) Immunoprecipitation assay
b) ChIP assay
c) GST-pull down assay
d) ELISA
e) DNAase Foot-printing assay
f) Western Blot, Gel Electrophoresis
g) Protein/DNA purification (also includes gel extraction, MiniMaxi prep), Protein/DNA quantification.
h) Protein Kinase Assay, Enzymatic assays, ligand-receptor
assays.
i) Gel shift.

Immunohistochemistry
a) In-situ hybridization assay (ISH)
b) Florescent in-situ hybridization (FISH)
c) Polytene Chromosome Squash preparation.
d) Immunofluorescence Labeling Methods.

Mouse experience: Extensive experience in handling and


maintaining mice.
a) Drug administration via intraperitoneal injection, osmotic
pumps and water bottles.
b) Urine collection.
c) Retro orbital bleeding, mandibular & tail bleeding.
d) Ear clipping, shaving, and surgery.
e) Extracting bone marrow and transplantation.

Clinical experience:
a) Handling patient samples with Sickle Cell Disease (blood,
plasma, serum, and urine).
b) ELISA (albumin, creatinine, various protein detection
assays).
c) Measuring urine osmolality using vapor pressure
Osmometer (Vapro Osmometer 5520).
d) Measuring reactive oxygen species (ROS).
e) Experience with commercial and experimental drugs
including Losartan, Angiotensin II receptor-1 blocker (Teva
Pharmaceuticals), Captopril, ACE-I inhibitor (West-ward
Pharmaceutical), PD123319, Angiotensin II receptor-2
blocker, C21 compound, Angiotensin II receptor-2 agonist,
Apocynin, Tempol, Quercitin.

Flow-cytometry experience: Experience in flow-cytometry


using BD FACSCanto II and MACS-Quant Analyzer.

Gene
knockout
techniques/siRNA
mediated
gene
knockdown:
a) Targeted mutagenesis to create Gene Knock-out based on
homologous recombination in Drosophila melanogaster.
b) Transient transfection of various cell lines by gene specific
siRNA plasmids for gene knockdown and by plasmids
containing gene-of interest for overexpression using Amaxa
Nucleofector technology and Licofectamine2000 mediated
transfection, RNAi MAX technique.

Mammalian/Mouse Cancer Cell Culture:


a) Handling and maintenance of mammalian/mouse Cancer
cell lines (MIN6, HEK293, Mouse Embryo fibroblasts, NIH3T3
cells) and human primary cells (Hematopoietic stem cells).
b) Knowledge and exposure in stable cell line generation.

RNA and Gene expression:


a) PCR, QPCR, QRT PCR
b) Vector plasmid construction
c) Primer designing, Expression cloning (transfection, restriction
digestion, ligation, transformation)
d) Luciferase Reporter assay
e) Promoter work (Cloning different lengths of gene promoter)
f) Microarray
4

g) Spectroscopy and RNA quantification.

Awards

Sequencing knowledge:
a) Experience in Nucleic acid sequencing,
b) Plasmid-DNA sequencing, and
c) Genomic data analysis, from private and public websites.

Microbiology techniques:
a) Microbiological culture
b) Microscopy
c) Biochemical Test: Kirby-Bauer antibiotic testing, Industrial
fermentation, various biochemical tests (Indole test, Voges
Proskauer test, Oxidase test), Bacteriological water analysis
d) Aseptic technique, Streaking, Blood culture, pure culture
techniques
e) Staining: Gram staining, ZiehlNeelsen staining, Endospore
staining, Capsule staining.

Instrument use
a) Operation of Electron Microscope
b) Dissection Microscope
c) Confocal microscope
d) Fluorescent microscope
e) Spectrophotometer
f) Plate Reader
g) Nanodrop
h) Sonicator

Other Biological model


a) Worked with Drosophila melanogaster.
b) Involved in fly maintenance, genetics, cross-strategy,
making fly food

American Society of Hematology (ASH) 2013, Achievement


Award for abstract Increased Oxidative Stress In Sickle Cell
Disease Activates The Renin-Angiotensin-TGF- pathway To
Mediate Sickle Nephropathy.
Post Doctoral Fellowship Award (2009-2011) NIDDK, National
Institutes of Health.
Mc Daniels Scholarship Travel Award 2008.
Louisiana State University, Graduate School Travel Award, April

Teaching
Experience

Computer skills,
Communication
skills, ability to
work in a team
environment &
other qualities

2008.
Louisiana State University, Graduate School Travel Award, April
2007.
Distinction award in Master of Science of the Microbiology in
the university examinations held during 2003, P.E.S. degree
college, Bangalore University.

Taught primarily undergraduate lab courses and also


graduate level courses for 5 years at the Louisiana State
University.
BIOL 1012 (GENERAL MICROBIOLOGY LAB) Lab designed to
learn basic laboratory skills, for handling and observing
microorganisms; demonstration of features of microorganisms.
BIOL 1208 (BIOLOGY LAB FOR SCIENCE MAJOR I) Primarily for
students majoring in science, agriculture, or education.
BIOL 2051 (GENERALMICROBIOLOGY LAB) Structure and
function of microbial cells and their relationship to people and
the environment.
BIOL 3090 (CELL BIOLOGY LECTURE COURSE) Molecular
description of cell structure and function.
Familiar in working with both Mac and Windows operating
systems. Expert with using PowerPoint, Excel, Word and Adobe
Photoshop.
Excellent communication skills and writing ability (author for
www.biocareers.com).
Talented public presentation abilities. Gave talks at various
university and research conferences. Lectured graduate and
undergraduate courses for more than 5 years.
Worked in projects, which involved multiple members. Value
working as a team, the compromises one requires to accept,
and the support one needs to offer to other colleagues to
move together and succeed as an entire group.
Autonomously capable to design experiments relevant to the
project, make independent scientific decisions based on
logical reasoning.
Can do multi-tasking and extremely punctual about finishing
experiments within the desired time frame.
Very methodical and likes adhering to principles and
guidelines.
6

Articles/Talks/
Presentations

Experience in supervising and mentoring undergraduates,


summer students and even fresh PhD candidates. Helped
them with their projects and posters.
Expertise in lab research, data collection/analysis and project.
management.
Experience in writing manuscripts for publications.
Extensive experience working with cross-functional scientific
and research teams.
Presented poster at the 55th American Society of Hematology
(ASH) Annual Meeting in New Orleans, LA, December 7-10,
2013.
Published
articles
as
a
scientific
author
for
www.biocareers.com.
Gave
Online
Webinars
for
www.biocareers.com.
Regularly gave talks at NIDDK, National Institutes of Health
seminar series.
Presented poster at the 49th Annual Drosophila Research
Conference, San Diego, CA, April2008 (Swarnava Roy1 and
Craig M. Hart Evidence for a broad role of BEAF in
maintaining global patterns of gene expression).
Presented poster at the 48th Annual Drosophila Research
Conference, Philadelphia, PA, April 2007 (Swarnava Roy1,
Matthew Gilbert and Craig M Hart (2007) Characterization of
BEAF mutations isolated by homologous recombination in
Drosophila. Genetics. April 15, 2007).
Gave a talk for the Bio Grads Symposium November 2007
(Swarnava Roy1 and Craig M Hart) Evidence for a broad role
of BEAF in maintaining global patterns of gene expression).
Presented poster for Bio Grads Symposium, November 2006
(Swarnava Roy1, Yian Yee Tan1 and Craig M Hart1 (2006) A
genetic screen supports a broad role for the drosophila
insulator proteins BEAF32A and BEAF32B in maintaining
patterns of gene expression).