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SPRING 2000
April 25 Lecture: Integration of Metabolism
A. Metabolism Basics
Strategy of metabolism is to form ATP, reducing power and biosynthetic building blocks
• ATP
o universal currency of energy,
o high phosphoryl group transfer potential
o hydrolysis of ATP is coupled with unfavorable reactions to make them
favorable
o generated by oxidation of food molecules
glucose, fatty acids, common intermediate is acetyl CoA
electrons carried by NADH and FADH2
• NADPH
o major electron donor in reductive biosynthesis
• Biomolecules constructed from small number of building blocks
o common intermediates for pathways
• Biosynthetic and degradative pathways almost always distinct
o fatty acid synthesis (cytosol); fatty acid degradation (mito. matrix)
B. Recurring Motifs
1. Allosteric interactions
2. Covalent modification
3. Enzyme levels
4. Compartmentation
1. Glycolysis
4. Gluconeogenesis
D. Key Junctions
Pyruvate
Acetyl CoA
2. Muscle
3. Adipose Tissue
4. Liver
• essential for providing fuel to brain, muscle, and other peripheral organs
• converts glucose to glycogen and breaks down glycogen to glucose
• Fatty acids derived from diet or synthesized by liver are secreted as very low
density lipoprotein (VLDL)
• During fasting converts fatty acids to ketone bodies
• Prefers keto acids derived from amino acids as fuel source