MCB 101 Exam 1 Information

Spring 2014
Exam Date: Friday, February 2, 2014
Exam !ime: ":00 # ":$0 %M
Exam &'a(e:
if you are in )e(tion): %, B, C, D or E # room 10* M+M
If you are in )e(tion): F, , or - # room 1*4 !-B-
!.e exam (o/er) materia' from experiment) 1 # ,
page) 1 # 01 of t.e Spring 2014 2ab Manua'3
MCB101 Introductory Microbiology Lab
Practice Exam 1
For each question choose the one best answer.
Multiple Choice
1) Which one of the following statements about bacteria in the environment is false?
A. A single gram of garden soil may contain a billion bacterial cells.
B. There are no bacteria found on clean, healthy human skin.
C. There are bacteria and mold spores drifting in the air, mostly on dust particles.
D. There are bacteria living in your mouth, even if you brush your teeth regularly.
E. A Petri dish of medium can become contaminated if you leave it open for too long.
2) When Staphylococcus epidermidis is incubated on a Petri dish of Phenolethanol agar,
individual cells grow into visible colonies. But when Esherichia coli is plated on this
medium, it dies. In this respect phenolethanol agar is an example of:
A. a general purpose medium B. a differential medium
C. a selective medium D. a defined medium
E. a complex medium
3) Many Gram negative bacteria can grow on MacConkey agar but the growth of
most Gram positive bacteria is inhibited.
What ingredient is used in MacConkey agar to cause this inhibition?
A. agar B. an antibiotic C. bile salts
D. lactose E. phenolethanol


4) When describing a bacterial colony growing on an agar plate, the elevation of
the colony is one of the traits that can be noted.
What is the term used to describe a colony that has this sort of elevation profile?

A. raised B. pulvinate C. craterform D. convex E. umbonate
5) Agar is:
A. a polymer extracted from algae that has no nutritional value.
B. a common source of nitrogen in liquid media.
C. toxic to most medically important bacteria.
D. a protein extracted from the hooves of cows.
E. degraded by most medically important bacteria.
6) What substance(s) in sucrose fermentation broth can act as a carbon source?
A. The carbon source in sucrose fermentation broth is carbon dioxide.
B. The only source for carbon in sucrose fermentation broth is sucrose.
C. There are two carbon sources in sucrose fermentation broth, sucrose and glucose.
D. There are two carbon sources in sucrose fermentation broth, sucrose and amino acids.
E. There are three carbon sources in this broth, sucrose, ammonium sulfate and vitamins.
7) Which one of the following statements best describes the appearance of a reliable
positive result for a carbohydrate fermentation test.
A. The medium is clear and does not change color.
B. The medium is cloudy and greenish-blue.
C. The medium is cloudy and yellow.
D. The medium is red.
E. The pH indicator dye has turned a color that reveals the presence of base.
8) The type of microscopy we use to examine bacteria that are stained by the
Gram stain procedure is called:
A. bright-field microscopy. B. fluorescent microscopy.
C. dark-field microscopy. D. transmission electron microscopy.
E. phase-contrast microscopy.
9) You are looking at an insect with a compound microscope. The ocular lens has a
magnification of 8X and the objective lens has a magnification of 2X.
What is the total magnification?
A. 2X B. 4X C. 8X D. 10X E. 16X
10) What is the diameter a typical coccus bacterium?
A. 1 x 10
-5
meters B. 1 x 10
-6
meters C. 1 x 10
-7
meters
D. 1 x 10
-8
meters E. 1 x 10
-9
meters
11) Which of the following statements about using amino acids in media for the
culturing of bacteria is true?
A. Amino acids can be used as a source of carbon by many common bacteria.
B. Amino acids can be used as a source of nitrogen by many common bacteria.
C. Amino acids can be broken down to provide energy.
D. All of the above (A C) are true.
E. Responses A and B are true, but not C.
12) What would be the term used to describe the shape of these bacteria?

A. sarcina B. streptobacillus C. diplococci
D. vibrio E. spirilla
13) Which one of the following statements about the Gram stain is false?
A. In the Gram stain, a dye called crystal violet is used to stain the cells purple.
B. Gram negative cells have thinner cell walls than Gram positive bacteria.
C. In the Gram stain, alcohol is used to kill the bacteria.
D. Gram positive cells end up being stained purple.
E. Gram negative cells end up being stained pink or red.
14) The Gram stain divides microorganisms into two groups, purple vs. red, on the basis
of differences in the:
A. presence of a capsule.
B. presence of an outer membrane.
C. thickness of the peptidoglycan layer of the cell wall.
D. presence of waxy mycolic acids in the cell wall.
E. presence of endospores.
15) What is the correct order in which these reagents are used in the Gram stain?
A. Crystal Violet, Ethanol, Iodine, Safranin,
B. Crystal Violet, Safranin, Ethanol, Iodine
C. Crystal Violet, Iodine, Ethanol, Safranin
D. Safranin, Iodine, Crystal Violet, Ethanol
E. Iodine, Safranin, Ethanol, Crystal Violet
16) You grow a pure culture of an unknown bacteria on a starch agar plate.
After 48 hours you flood the plate with iodine. Most of the plate turns
dark blue except for a clear zone around the bacterial growth.
What does this mean?
A. The bacteria excrete amylase.
B. The bacteria excrete acid.
C. The bacteria excrete basic waste products.
D. The bacteria degrade agar.
E. The bacteria degrade amino acids.
17) You streak an unknown bacterium on a blood agar plate. After incubation
you notice a distinct zone around the colonies that is completely clear.
What does this mean?
A. The bacteria excrete acid.
B. The bacteria excrete an enzyme that degrades protein.
C. The bacteria are alpha-hemolytic.
D. The bacteria are beta-hemolytic.
E. The bacteria are gamma-hemolytic.
18) All of the following statements describe Escherichia coli except one.
Which one of the following statements is false?
A. Escherichia coli is a Gram positive coccus.
B. Escherichia coli can grow on MacConkey agar.
C. Most samples of mammalian feces contain some Escherichia coli.
D. Escherichia coli does not produce amylase, lipase or gelatinase.
E. Escherichia coli is a common inhabitant of mammalian large intestines.
19) A mutant strain of bacteria that has an additional nutritional requirement that is
not seen in the wildtype strain is called:
A. an autotroph B. a heterotroph C. a prototroph
D. an auxotroph E. a phototroph
20) Which one of the following statements about the O-F glucose test is true?
A. Enteric bacteria like Escherichia coli require oxygen to make acid from glucose.
B. Pseudomonas bacteria produce acid from glucose both with and without air.
C. Pseudomonas bacteria cause neither of the two tubes to turn yellow.
D. Pseudomonas bacteria cause both tubes to turn yellow.
E. Enteric bacteria like Escherichia coli cause both tubes to turn yellow.
Matching Some responses are used more than once, others are not used at all.
21 24) MATCH each medium with its purpose.
A. O-F glucose tube B. starch agar C. gelatin agar
D. sucrose fermentation broth E. egg yolk agar
21) use to etermine if a bacterium can gro! by using fermentati"e metabolism
22) use to etect the #rouction of e$tracellular amylase en%yme
2&) use to etect the #rouction of e$tracellular li#ase en%yme
2') use to see if a bacterial strain can make aci from table sugar !hen gro!ing
anaerobically
2( ) 2*) +A,C- each chemical reagent. meia ingreient or item !ith its #ur#ose.
A. glucose B. ammonium sulfate C. sterile mineral oil
D. ioine E. yeast e$tract
2() use to etect the hyrolysis of gelatin
2/) use to kee# o$ygen out of broth or semisoli meia
20) use as a source of "itamins. amino acis an other nutrients in rich com#le$ meia
2*) use to etect starch hyrolysis in the amylase test
21 ) &&) +A,C- each bacteriological test !ith the a##earance of a #ositi"e result.
For this section. res#onses may be use more than once.
A##earance of a 2ositi"e 3esult
A. a clear %one is seen aroun the bacterial gro!th after aition of a chemical reagent
B. a clear %one is seen aroun the bacterial gro!th after incubation
C. the meium turns blue
D. the meium turns yello!
21) Amylase ,est
&4) ,he bacteria make aci because they ferment a sugar.
&1) -emolysis ,est
&2) 5i#ase ,est
&&) ,he bacteria gro! on 6immon7s citrate agar.
&' ) &0) 8n this matching section you are to #ut the ste#s of the #roceure in the #ro#er
orer. ,he to#ic is9
&reparation of a Smear of Ba(teria on a Mi(ro)(ope S'ide for t.e Simp'e Stain
A. the first ste# B. the secon ste# C. the thir ste# D. the fourth ste#
&') Clean the slie an #lace a small ro# of !ater on the slie.
&() Allo! the slie to ry at room tem#erature.
&/) :se a sterile inoculating loo# to s#rea a tiny sam#le of bacteria on the slie.
&0) ;uickly #ass the slie through the flame to heat fi$ the cells to the glass.
&* ) '() !rue4 Fa')e 5ue)tion)
&*) <hen you label a 2etri ish culture. you shoul al!ays !rite the information
on the li of the #late.
&1) =ram negati"e bacteria that ferment lactose form #ale !hite colonies on E+B agar.
'4) At a #- of 0.4. the inicator ye bromthymol blue is green
'1) 5uria agar is a efine meium that contains only glucose an inorganic salts.
42) In the gelatinase test, the plate is flooded with ammonium sulfate after incubation.
43) Iodine forms a blue-black complex when it binds to lipids found in egg yolk.
44) There are no vitamins or amino acids in Simmons citrate agar.
'() <hen you #ut a 2etri ish into an incubator you shoul al!ays #ut the agar sie u#.
'/ ) '1) Mat(. the reagents or #roceures !ith the stain or use.
A. congo re B. malachite green >!ith heat for ( minutes)
C. tannic aci D. safranin
'/) use as a morant for the flagella stain
'0) use to stain enos#ores
'*) use as a counterstain
'1) use to stain the backgroun in the ca#sule stain
(4 ) (1) multi#le choice ?uestions
(4) <hich of the follo!ing bacterial s#ecies can make heat resistant enos#ores
an be gro!n in aerobic conitions@
A. Pseudomonas aeruginosa B. Bacillus cereus C. Clostridium tetani
D .Streptococcus pyogenes E. Esherichia coli
(1) <hich of the follo!ing bacterial s#ecies makes a ca#sule !hich is an im#ortant
"irulence factor@
A. Streptococcus pneumonia
B. Klebsiella pneumonia
C. Pseudomonas aeruginosa
D. Neisseria meningitidis
E. all of the abo"e
Answer Key
1) B 18) A 35) C
2) C 19) D 36) B
3) C 20) E 37) D
4) E 21) A 38) F
5) A 22) B 39) F
6) D 23) E 40) T
7) C 24) D 41) F
8) A 25) B 42) T
9) E 26) C 43) F
10) B 27) E 44) T
11) D 28) D 45) T
12) D 29) A 46) C
13) C 30) D 47) B
14) C 31) B 48) D
15) C 32) B 49) A
16) A 33) C 50) B
17) D 34) A 51) E