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Feline Heartworm-Feline Leukemia Virus Antigen-

Feline Immunodeficiency Virus Antibody Test Kit


For veterinary use only.

SNAP® Feline Triple™


In vitro diagnostic for the detection of Dirofilaria immitis antigen, antigen to feline leukemia virus and
antibody to feline immunodeficiency virus in feline serum, plasma or whole blood.

Precautions and Warnings


• All wastes should be properly decontaminated prior to disposal.
• Do not mix components from kits with different lot numbers.
• Do not use a SNAP device that has been activated prior to the addition of sample.

Storage
Store devices and test reagents at 2°– 7°C until the expiration date. All components must be at room
temperature (15°– 30°C) before running the test—do not heat.

Kit Components
Each kit contains one 8 mL bottle of anti-HW, anti-FeLV and FIV:HRPO conjugate and one reagent
rack, as well as 5, 15 or 30: transfer pipettes, sample tubes and SNAP devices. Each SNAP device
contains 0.4 mL of wash solution and 0.6 mL of substrate solution.

Sample Information
• Samples must be at room temperature (15°– 30°C) before beginning the test procedure.
• Serum, plasma or anticoagulated whole blood (e.g., EDTA, heparin), either fresh or stored at
2°–7°C for up to one week, can be used.
• For longer storage, serum or plasma can be frozen (-20°C or colder) and then recentrifuged
before use.
• Hemolyzed or lipemic samples will not affect results.

Test Procedure
1. Allow all components to equilibrate at room temperature (15°– 30°C) for 30 minutes before use.
Do not heat.
2
2. Using the pipette provided,
dispense 3 drops of sample 3
into a new sample tube.

3. Holding the bottle vertical, add 3


4 drops of conjugate to the
sample tube. 4

4. Cap the sample tube and mix it 4


thoroughly by inverting it 3–5 times.

5. Place the device on a horizontal surface.


Add the entire contents of the sample
5
tube to the sample well, being careful not to
Sample Well
splash the contents outside of the sample well.
Result Window
The sample will flow across the result window,
reaching the activation circle in 30– 60 seconds. Activation Circle

Some sample may remain in the sample well. Activator

6. When color FIRST appears in the activation circle,


push the activator firmly until it is flush with the
device body.

6
Not fully
depressed
Right Wrong

Note: Some samples may not flow to the activation circle within 60 seconds, and, therefore, the
circle may not turn color. In this case, press the activator after the sample has flowed across the
result window.
7. Test results must be read at 10 minutes from the time of activation.
NOTE: The positive control may develop sooner, but results are not complete until 10 minutes.

Interpreting Test Results

Positive control
Positive Result
Any color development in the sample spots
indicates the presence of FIV antibody, FeLV FIV antibody FeLV antigen
antigen or heartworm antigen in the sample.

Heartworm antigen

Negative Result
Only the positive control spot develops color.

Invalid Results
• Background—If the sample is allowed to flow past the activation circle, background color may
result. Some background color is normal. However, if colored background obscures the test
result, repeat the test.
• No Color Development—If the positive control does not develop color, repeat the test.

Sensitivity and Specificity of SNAP Feline Triple


Sample Size
SNAP Feline Triple/Reference Test
Relative Sensitivity and Specificity Kappa
Comparison Test +/+ -/+ +/- -/- Total Sample Type 95% Confidence Limit Statistic
Heartworm Necropsy/ Sen., 86.7% (95% CL 69.5%–95.2%)
26 4 0 208 238 Serum/Plasma 0.92
PetChek® Heartworm PF Ag1 Spec., 100% (95% CL 97.8%–100%)
Sen., 100% (95% CL 95.3%–100%)
PetChek FeLV Ag2 97 0 3 208 308 Serum/Plasma 0.98
Spec., 98.6% (95% CL 95.7%–99.7%)
Sen., 99.2% (95% CL 95%–100%)
PetChek FIV Ab3 123 1 0 208 332 Serum/Plasma 0.99
Spec., 100% (95% CL 97.8%–100%)
1. Necropsy/PetChek Heartworm PF Antigen Test Kit (5018.02)
2. PetChek Feline Leukemia Virus Antigen Test Kit (5028.01)
3. PetChek Feline Immunodeficiency Virus Antibody Test Kit (5036.00)
CL = confidence limit

IDEXX Technical Support


USA/Canada 1-800-248-2483 • Europe 00800 1234 3399 • Australia 1800-655-978
idexx.com

U.S. Vet. License No. 313


Product Code 502C.00
SNAP, Feline Triple and PetChek are trademarks or registered trademarks of
IDEXX Laboratories, Inc. in the United States and/or other countries.
Covered by U.S. Patent Nos. 4,713,325; 5,037,753; 5,118,602; 5,565,319;
5,627,026; 5,726,010; 5,726,013; 5,750,333 and/or 6,007,999. Other U.S.
and/or foreign patents issued or pending. One IDEXX Drive
Westbrook, Maine 04092 USA
© 2007 IDEXX Laboratories, Inc. All rights reserved. • 06-09422-02 idexx.com

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