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Coverage of ASR Reagents


Analyte Specific Reagents (ASR), as defined by FDA 21 CFR 864.4020, are antibodies, both polyclonal and monoclonal, specific receptor
proteins, ligands, nucleic acid sequences, and similar reagents which, through specific binding or chemical reaction with substances in a specimen,
are intended to use in a diagnostic application for identification and quantification of an individual chemical substance or ligand in biological specimens.
An ASR is the active ingredient of an in-house test; it is the responsibility of the clinical laboratory using the ASR to develop the test and to establish and maintain
its performance.
Beckman Coulter provides a variety of cellular analysis reagents to meet the needs of todays flow cytometry clinical laboratories. In the present table, only directly
conjugated reagents that are ASR are described. These comprise hundreds of high quality products allowing easier test development and sample preparation flexibility.
Marker

Clone

Flourochrome
Max Emission Wavelength
Excitation Source

CD1a
CD2

BL6
39C1.5

IgG1 (mouse)
IgG2a (rat)

CD3

UCHT1

IgG1 (mouse)

CD14
CD15

SFCI12T4D11 (T4)
13B8.2
BL1a
3A1E-12H7
8H8.1
SFCI21Thy2D3 (T8)
B9.11
2ST8.5H7
ALB6
J5
ALB1
ALB2
25.3
Bear1
BU15
366 (MY7)
SJ1D1
Immu103.44
RMO52
80H5

IgG1 (mouse)
IgG1 (mouse)
IgG2a (mouse)
IgG2b (mouse)
IgG2a (mouse)
IgG1 (mouse)
IgG1 (mouse)
IgG2a (mouse)
IgG1 (mouse)
IgG2a (mouse)
IgG1 (mouse)
IgG2a (mouse)
IgG1 (mouse)
IgG1 (mouse)
IgG1 (mouse)
IgG1 (mouse)
IgG1 (mouse)
IgG1 (mouse)
IgG2a (mouse)
IgM (mouse)

CD16

3G8

IgG1 (mouse)

CD16b
CD18

1D3
7E4
HD237 (B4 lytic)
J3-119
B9E9 (HRC20)
BL13
HD239 (B3)
SJ10.1H11
9P25
HD50 (B6)
ALB9
1HT44H3 (IL-2R1)
B1.49.9
1A4CD27
CD28.2
K20
HRS4
5.6E
906 (MY9)
D3HL60.251
581
QBEnd10
QBEnd10 + Immu133 + Immu409
FA6.152

IgM (mouse)
IgG1 (mouse)
IgG2b (mouse)
IgG1 (mouse)
IgG2a (mouse)
IgG1 (mouse)
IgG2b (mouse)
IgG1 (mouse)
IgG1 (mouse)
IgG2b (mouse)
IgG1 (mouse)
IgG2a (mouse)
IgG2a (mouse)
IgG1 (mouse)
IgG1 (mouse)
IgG2a (mouse)
IgG1 (mouse)
IgG1 (mouse)
IgG2b (mouse)
IgG1 (mouse)
IgG1 (mouse)
IgG1 (mouse)
IgG1 + IgG2a (mouse)
IgG1 (mouse)

LS198-4-3

IgG1 (mouse)

T16
MAB89
P2
SZ22
69 (Plt-1)
SZ1
SZ2
DFT1
J.173
J.33
KC56 (T-200)
Immu19.2
2H4
ALB11
UCHL1
J4.57
HP2/1
SAM1
84H10
N901 (NKH-1)
NC1
AICD58
P282E
SZ21
DREG56
CLBThromb/6
CLBGran/12
22
88H7
80H3
KOR-SA3544
TP1.55.3
YDJ1.2.2
HM47
CB3-1
MAB104
HB15a
HA5.2B7
F15-42-1-5
Thy1/310
7C11
2G5
95C3
104D2D1
SSDCLY107D2
R34.34
SF1.340
B-A38
TRAP-1
GL183
Z27.3.7
FES172
Z199
11E4B-7-6
UIC2
7.1
2.7A6A3 (APO2.7)
J1B3
FMC7
B9.12.1
Immu-357
B8.12.2
45.15
N7.48A
4D9
Polyclonal
Polyclonal
CLB-MPO-1
IP26A
IMMU510
HT1 + HT4 + HT8 + HT9
IPM2 (188)
N/A
N/A
N/A
N/A

IgG1 (mouse)
IgG1 (mouse)
IgG1 (mouse)
IgG1 (mouse)
IgM (mouse)
IgG2a (mouse)
IgG1 (mouse)
IgG1 (mouse)
IgG1 (mouse)
IgG1 (mouse)
IgG1 (mouse)
IgG1 (mouse)
IgG1 (mouse)
IgG1 (mouse)
IgG2a (mouse)
IgG1 (mouse)
IgG1 (mouse)
IgG2b (mouse)
IgG1 (mouse)
IgG1 (mouse)
IgM (mouse)
IgG2a (mouse)
IgG2a (mouse)
IgG1 (mouse)
IgG1 (mouse)
IgG1 (mouse)
IgG1 (mouse)
IgG1 (mouse)
IgM (mouse)
IgG1 (mouse)
IgG1 (mouse)
IgG2b (mouse)
IgG1 (mouse)
IgG1 (mouse)
IgG1 (mouse)
IgG1 (mouse)
IgG2b (mouse)
IgG2b (mouse)
IgG1 (mouse)
IgG1 (mouse)
IgM (mouse)
IgG2a (mouse)
IgG1 (mouse)
IgG1 (mouse)
IgG1 (mouse)
IgG1 (mouse)
IgG1 (mouse)
IgG1 (mouse)
IgG1 (mouse)
IgG1 (mouse)
IgG1 (mouse)
IgG2a (mouse)
IgG2b (mouse)
IgG1 (mouse)
IgG2a (mouse)
IgG1 (mouse)
IgG1 (mouse)
IgG1 (mouse)
IgM (mouse)
IgG2a (mouse)
IgG1 (mouse)
IgG2b (mouse)
IgG1 (mouse)
IgG2a (mouse)
IgG1 (mouse)
IgG (rabbit)
IgG (rabbit)
IgG2a (mouse)
IgG1 (mouse)
IgG1 (mouse)
IgG1 + IgG2a (mouse)
IgG1 (mouse)
IgG1 (mouse)
IgG2a (mouse)
IgG2b (mouse)
IgM (mouse)

CD4
CD5
CD7
CD8
CD8b
CD9
CD10
CD11a
CD11b
CD11c
CD13

CD19
CD20
CD21
CD22
CD23
CD24
CD25
CD27
CD28
CD29
CD30
CD31
CD33
CD34
CD36
CD38
CD40
CD41
CD42a
CD42b
CD43
CD44
CD45
CD45RA
CD45R0
CD48
CD49d
CD49e
CD54
CD56
CD57
CD58
CD59
CD61
CD62L
CD62P
CD63
CD64
CD65
CD66b
CD66c
CD69
CD71
CD79a
CD79b
CD80
CD83
CD86 (B7-2)
CD90 (Thy-1)
CD95 (Fas)
CD103 (HML-1)
CD117 (c-kit)
CD123 (IL-3 Ra)
CD127 (IL-7 Ra)
CD135 (FLT3)
CD138 (Syndecan-1)
CD154 (CD40-Ligand)
CD158b1/b2,j
CD158e1/e2
CD158i
CD159a (NKG2A)
CD235a (Glycophorin A)
CD243 (P-glycoprotein)
7.1 (NG2 Antigen)
APO2.7 (7A6 Antigen)
Cytokeratin
FMC7 (CD20 special epitope)
HLA-ABC
HLA-DR
IFN-g
IL-2
IL-4
Kappa Light Chain
Lambda Light Chain
Myeloperoxidase (MPO)
TCR Pan a/b
TCR Pan g/d
TdT
TNFa
IgG1 (Isotypic Control)
IgG2a (Isotypic Control)
IgG2b (Isotypic Control)
IgM (Isotypic Control)

Krome
Orange

FITC

PE or RD1

ECD

PC5

PC5.5

PC7

APC

450 nm

528 nm

525 nm

575 nm

620 nm

675 nm

695 nm

770 nm

660 nm

Violet Source (405 nm)

A93687

Blue Source (488 nm)

IM0442U

IM1942U
IM0443U

IM1281U

IM1282U

IM0448U
IM0468U
6603824
IM0585U

IM0449U
IM0469U
6603822
IM1429U

IM0451U

IM0452U
IM2217U

IM2635U
6607010

APC-Alexa
Fluor 750

725 nm

770 nm

Red Source (640 nm)

IM2634U
IM2705U

APC-Alexa
Fluor 700

A51074

A60794

A66327

IM2467U

A70203

IM2468U
A60790

A66329

6604727
A82789
A82790

A96417

A82791

IM1755U
6604107
IM2720U
IM0471U
IM0860U
IM0530U

IM0778U
IM0645U
IM1423U
IM0814U
6604894
IM2353U
IM1568U

A74775
A82792

A86355
A74777

IM1284U
IM1455U
IM0473U
IM0779U
IM0529U
6604368

A93689

6603200
IM0478U

A82944

IM1236U
IM0791U

6604120
IM1915U

A33096
A70202
6604728

IM3608U

IM2636U
IM2637U
IM3613U
6607011
IM2638U

A46526

IM2721U

A46527

IM1238U

A33097
IM2707U

IM2639U
IM2640U
IM2641U

A33098

IM2642U

6604551
IM2708U
IM3607U

IM2643U
IM2644U

A79389
A70204

A87782

A46528

A87783
IM2580U

A74765

A96416

IM0649U
IM1756U
6602993
IM1757U
IM0648U
IM3264U
IM1219U
IM0782U
6604104

A89310

A86052
A89312

A66330

A78838
A66331

IM1570U
IM1285U
IM1451U
A32536
6604428
IM1835U
A33099
6604426
IM1428U
6604422
IM0479U

A66328

IM3628U
IM3629U

IM2470U
A51076

A78837

A80712

A74788

A60791
A69964

A89311
A70207

IM3609U

A87785
IM2646U

A79386

A52882
A54823

A86356

IM2071U

6604121
IM1179U
IM1871U
IM1250U
IM1459U

A87939

IM2709U

IM2647U
IM2648U
IM2650U

A70198

A54824
A51077

IM0766U

IM0775U

A78836

A66332

A86353

A54822
A80249

IM2033U
IM1431U
6604111
IM1135U
IM1870U

A78835
A70201

IM2469U

IM1433U
IM2581U
IM1760U
6602989
IM1427U
IM0650U
IM1954U

A51075

IM2471U
IM2472U

A86354

A70200
A89309

A87786
IM2371U
6604928
IM1832U
IM1936U
IM1416U

IM1417U
A32560
A32537
IM2078U
6603839

IM2651U

A70205

A54189

A60792

A86049

A89307
IM2710U

IM2653U

A54139

IM3548U

IM2473U

A71117

A71119

IM2652U
A82946

IM2711U
IM0584U
IM1247U
IM1837U
IM1404U
IM1854U
IM0726U

A74779

IM0466U
IM1218U
IM3457U
IM1758U
IM1231U
A51079
IM1165U
IM1604U
IM1654U
IM0531U
IM2039U
IM0483U

IM1853U
IM2410U
IM1839U

A86050

IM1834U
IM1307U

IM2712U

IM1239U
IM2073U

A82943

IM2654U

IM2713U

IM2655U

IM2214U
IM1759U
IM1914U
IM3601U

A79388

A51078

IM2474U

IM3606U

IM2357U
IM1943U
IM2001U
IM2221U
IM1612U
IM1976U
IM2218U
IM2729U
IM1840U
IM3600U
IM2446U

A89308

IM2656U

A80710

A80711
A89313

IM3456U

A60793
A87784

IM3240U

IM1856U
IM1360U
IM2732U
A32535
IM1980U
IM2234U
A40316
IM2216U
IM2278U

IM2212U

A74781

IM2356U
IM1364U
IM1838U
IM1638U
IM0463U
IM2716U

IM2657U
IM2733U

A66333

A64617

A86051
A64618

A40317

A71116
A87787

A60795
A60796
A60797

IM3291U
IM2211U
IM2370U
IM3454U
IM2088U

A71564

IM1639U
IM0464U
IM2717U
IM2718U
IM2719U

IM2659U

A89314

A40579

A64828
IM1874U
IM1571U
IM3524U
A74766

IM0639U
A12690

A64827
IM3455U
A39499
IM1418U
IM3279U
IM0670U
A09141
6603852

A39500
IM2662U

IM2714U
A09144
6604700

IM2663U
A09147

A62834
A12692

IM2475U
A12694

A71118

A71120

IM1269U

ASR: Analyte Specific Reagent. Analytical and performance characteristics are not established. September 2010 Edition of the ASR Reagents Table.


Beckman Coulter and the stylized logo are registered trademarks of Beckman Coulter, Inc.
Krome Orange is a trademark of Beckman Coulter, Inc.
Pacific Blue is a registered trademark of and licensed under patents assigned to Molecular Probes, Inc.
Alexa Fluor is a registered mark of and is licensed under patents assigned to Molecular Probes, Inc.

B2010-11474 P-14200A

Pacific
Blue

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