Biophen PC on ACL7000 (research software) 1/1 D.750.

07/B/1202/1205/ACL7000
HYPHEN BioMed Version 3 04/02/2010 LLL
B Bi io op ph he en n P PC (A221202/A221205) C

o on n A AC CL L7 70 00 00 0 ( (r re es se ea ar rc ch h s so of ft tw wa ar re e) )

NOTE : reconstitution, preparation, caIibration and controIs, expression of resuIts: usuaI
cautions and reaIization according to the specific technicaI insert D.750.02/BI/1202 or
/1205.

Reagents are reconstituted as per the insert, weII equiIibrated, and preheated at 37C


PHASE
CONDTON
OPERATON PARAMETERS
1
st
loading
step
YES
Reagent loading (pos 18) 0l
Washing after reagent loading (pos 18) 0

Diluent (pos DL) 0L
Specimen (working diIution 1 : 2 in
physioIogicaI saIine)
25I
Reagent (naceIIe 2) Reagent R1 Protac 100L
Washing between specimen 3

Optical ref. (cycle abs.)
Diluent (pos DL)
Reagent (nacelle 2)

80L
80L
Washing (at the end of loading) 3

ncubation time (T1) 60 s
2
nd
loading
step
YES Diluent (pos DL) 0L
Specimen 0L
Washing between specimen 0

Reagent (naceIIe 3) Reagent R2 substrate 100L
Washing after reagent loading 3

Mix time (T2) 300 s
Activation time (T3) 0 s
3
rd
loading
step
NO Reagent (pos 17) 0L
Washing after reagent loading 0

Mix time (T4) 0 s
Activation time (T5) 0 s
Washing NO Reagent (pos 1) 0L
Reagent (pos 16) 0L

Cycles number 1
Washing at the end of loading 1
Levels
detection
NO
Acquisition
parameters
YES Ramp yes
nter-ramp interval (T6) 1s
Delay before acquisition (T7) 5s
Acquisition time (T8) 300s

Canal used 405nm
Rotation speed 1200 rpm





Biophen PC on ACL7000 (research software) 2/1 D.750.07/B/1202/1205/ACL7000
HYPHEN BioMed Version 3 04/02/2010 LLL




Example of obtained calibration curve :










Note: The stability at 2-8C and at room temperature (18-25C) claimed are validated on
reconstituted vials kept closed, protected from any evaporation or contamination.
Stabilities of reagents on each automate must be controlled, adjusted and validated under each
exact laboratory working conditions.
% PC A405
92% (1 /2 dil)
0.59
46%
0.32
23%
0.17
11%
0.09
0.9%
0.003
r
2
( linA405-lin conc)

0.998
Biophen PC sur ACL7000 (logiciel recherche) 1/2 D.750.06/B/1202/1205/ACL7000
HYPHEN BioMed Version 3 04/02/2010 LLL

B Bi io op ph he en n P PC (A221202/A221205) C
s su ur r A AC CL L7 70 00 00 0 ( (l lo og gi ic ci ie el l r re ec ch he er rc ch he e) )

NOTE : reconstitution, prparation, caIibration et contrIe, expression des rsuItats :
prcautions d'usage, et raIiser seIon Ies indications de Ia notice technique
correspondante D.750.01/BI/1202 ou /1205.

Ractifs repris seIon notice, quiIibrs et prchauffs 37C

PHASE
CONDTON
OPERATON PARAMETRES
1ere tape de
chargement
OU
Chargement ractif (pos 18) 0l
Cycle nettoyage aprs ractif (pos 18) 0

Diluant (pos DL) 0L
Specimen (diIution de travaiI : 1/2 en
srum physioIogique)
25I
Ractif (naceIIe 2) Ractif R1 Protac 100L
Nettoyage entre chantillons 3

Ref Optique (cycle abs.)
Diluant (pos DL)
Ractif (nacelle 2)

80L
80L
Nettoyage (fin de chargement) 3

Temps d'ncubation (T1) 60 s
2eme tape
de
chargement
OU Diluant (pos DL) 0L
Specimen 0L
Nettoyage entre specimens 0

Ractif (naceIIe 3) Ractif R2 substrat 100L
Nettoyage (fin de chargement) 3

Temps de mlange (T2) 300 s
Temps d'Activation (T3) 0 s
3eme tape
de
chargement
NON Ractif (pos 17) 0L
Nettoyage (fin de chargement) 0

Temps de mlange (T4) 0 s
Temps d'activation (T5) 0 s
Nettoyage NON Ractif (pos 1) 0L
Ractif (pos 16) 0L

Nombre de Cycles 1
Nettoyage (fin de chargement) 1
Dtection de
niveaux
NON
Paramtres
d'acquisition
OU Rampe yes
ntervalle nter-rampe (T6) 1s
Dlai avant acquisition (T7) 5s
Temps d'Acquisition (T8) 300s

Canal utilis 405nm
Vitesse de Rotation 1200 rpm






Biophen PC sur ACL7000 (logiciel recherche) 2/2 D.750.06/B/1202/1205/ACL7000
HYPHEN BioMed Version 3 04/02/2010 LLL




Exemple de calibration obtenue :

% PC A405
92% (1 /2 dil)
0.59
46%
0.32
23%
0.17
11%
0.09
0.9%
0.003
r
2
( linA405-lin conc)

0.998


Nota: Les stabilits 2-8C et TA ont t obtenues sur des flacons reconstitus, conservs ferms,
exempts de tout risque d'vaporation ou de contamination. Chaque laboratoire doit contrler,
ajuster et valider selon ses conditions exactes de travail, la stabilit bord de chaque automate.