Experiments in Biochemistry/Molecular Biology

I. Techniques and Procedures

Chromatography

A laboratory experiment to demonstrate the principles of bio-ligand affinity
chromatography: the purification of chicken muscle AMP aminohydrolase
by phosphocellulose chromatography, M. Hahn and H. Husic, Biochem.
Educ. 17 9-9! "#9$9%.
&emonstration of chromatographic techni'ues using minicolumns, &. Miller et
al., Biochem. Educ. 1! ((-( "#99#%.

)eparation utili*ing gel filtration and ion-exchange chromatography, ). +usso
and A. +adcliffe, J. Chem. Educ. "# #!$-#,# "#99#%.
An inexpensi-e gel-filtration chromatography experiment, H. +o.e, J. Chem.
Educ. 7$ #/-#, "#99(%.
0solation of lactoferrin by immobili*ed metal ion affinity chromatography
"0MA1%, A. 1al-o and 2. 3atista-4iere, Biochem. Educ. %% /5-/6 "#99%.
+apid microscale isolation and purification of yeast alcohol dehydrogenase
using 1ibacron blue affinity chromatography, 1. Morgan and 7. Moir, J.
Chem. Educ. 7& #55-#5# "#99!%.
A Problem-sol-ing approach to chromatography in the biochemistry lab,
2. 8orga, J. Chem. Educ. 77 6!-6!! "6555%.
1hromatofocusing, 9. Mako-ec, Biochem. Mol. Biol. Educ. %# 65(-65!
"6555%.
Survey of biochemical separation techniques, M. Nilsson, J. Chem. Educ.
84,112-115 (200!.
"hromato#raphic separation of t$o proteins, %. S&eberenyi, Biochem. Mol.
Biol. Educ. 35, 1-2 (200!.
'()" *etermination of ca+eine an* para,anthine in urine- .n assay for
cytochrome (/50 1.2 activity, ). 0ur#e an* 1. 0let2e, Biochem. Mol. Biol.
Educ. 35, 134-1// (200!.
1
Computational Biochemistry
:7;<P=>9: a microcomputer assisted program for teaching en*yme
kinetics, 2. =eone et al., Biochem. Educ. %& (/-(, "#99/%.

Molecular modeling and experimental -erification of the relati-e stability of
&7A base pairs: a biochemistry laboratory experiment, ). 3urgess et al.,
Biochem. Educ. %' 6(5-6(# "#99!%.
)tructural analysis and modeling of proteins on the .eb, &. =eon, ).
?ridil, and @. Miranda, J. Chem. Educ. 7( ,(#-,( "#99$%.
9eaching 3ioinformatics, H. )alter, Biochem. Educ. %" (-#5 "#99$%.
?sing the computer in biochemical research, +. 3oyer, Modern Experimental
Biochemistry, 3
rd
ed., p. 6##-66/ "6555%, 3enAamin 1ummings ")an 2rancisco%.
0ntegrating computational chemistry into a proAect-oriented biochemistry
laboratory experience: a ne. t.ist on the lyso*yme experiment. +. Peterson,
@. 1ox, J. Chem. Educ. 7# #//#-#/// "655#%.
0ntegrating 0nternet assignments into a 3iochemistryBMolecular 3iology
laboratory course, +. Caspar, Biochem. Mol. Biol. Educ. &$ (!-(9 "6556%.
0ntroduction to interpretation of stochastic parameters: computer-intensi-e
procedures for e-aluation of data in en*yme kinetics, C. 9enekedAie- and
C. Cole-, Biochem. Mol. Biol. Educ. &$ #-#$ "6556%.
3ioMoleculesAli-e.org: 9he biochemistry and molecular biology digital
library update, P. 1raig, Biochem. Mol. Biol. Educ. &1 #/#-#/6 "655(%.
(hotochemical o,i*ation of bilirubin to biliver*in, 5. "oleman, J. Chem.
Educ. 83, 1326-1330 (2007!.
8tili&ation of inte#rate* Michaelis-Menten equation to *etermine 2inetic
constants, 9. :e&erra an* .. ;ias, Biochem. Mol. Biol. Educ. 35, 1/5-150
(200!.
Molecular mo*els of ;N., 5. "oleman, J. Chem. Educ. 84, 406-410
(200!.
Molecular mo*els of resveratrol, 5. "oleman, J. Chem. Educ. 84, 1171-
1172 (200!.
)yso&yme thermal *enaturation an* self-interaction, %. Sch$inefus et al.,
J. Chem. Educ. 85, 11-120 (2004!.
2
<ertical an* hori&ontal inte#ration of bioinformatics e*ucation, ). 0ur#e et al.,
Biochem. Mol. Biol. Educ. 37, 27-37 (2006!.
Electrophoresis
)&)-agarose gel electrophoresis in a simple procedure for determining high
molecular .eight protein oligomeri*ation, M. 3ro.nleader et al., Biochem.
Educ. %% #//-#/$ "#99%.
1apillary electrophoresis: a fast and simple method for the determination of
the amino acid composition of proteins, P. Deber and &. 3uck, J. Chem.
Educ. 71 !59-!## "#99%.
An inexpensi-e agarose gel electrophoresis system, &. 3etsch and @. 3eard,
Biochem. Educ. %7 $-/5 "#999%.
:lectrophoretic transfer techni'ue to detect and identify amylases in
polyacrylamide gels, 4. Mulimani and ). 9hippes.amy, Biochem. Mol.
Biol. Educ. %! 6/5-6/ "655#%.
&etermination of a diffusion coefficient by capillary electrophoresis, C.
Dilliams, 3. Adhyaru, 0. 8erman, and 9. +ussell, J. Chem. Educ. 7! #,/-
#,! "6556%.
Agarose gel electrophoresis system in the classroom. @. &e Mattos et al.
Biochem. Mol. Biol. Educ. &% 6/-6/, "655%.
An )&)-PA8: examination of protein 'uaternary structure. @. Po.ers et al.
J. Chem. Educ. #% 9(-9/ "655/%.
;etermination of the subunit molecular mass an* composition of alcohol
*ehy*ro#enase by S;S-(.=>, :. Nash, J. Chem. Educ. 84, 1504-1511
(200!.
Metal-cataly&e* cleava#e of t9N.-(he, S. 1ir2 et al., J. Chem. Educ. 85,
77-74 (2004!.

)eneral Techniques *solutions statistics etc.+
Preparing solutions in the biochemistry lab, @. +isley, J. Chem. Educ.
"# #5/-#5// "#99#%.
3
Automated data collection from manual Hitachi model ?-##55 spectrophoto-
meters and analysis by means of :xcel spreadsheets, &. 9itheridge et al.,
Biochem. Educ. %& 9#-9! "#99/%.
1oncepts and skills in the biochemistryBmolecular biology lab, +. 3oyer,
Biochem. Mol. Biol. Educ. &1 #56-#5/ "655(%.
?eachin# un*er#ra*uates to $rite scienti@c papers, ). ?omas2a, ASBMB
Today, May, 20-21 (200!.
,igand Binding
)pectrophotometric determination of the binding constants of succinate and
chloride to glutamic oxalacetic transaminase, A. Parody-Morreale et al., J.
Chem. Educ. "7 9$$-99# "#995%.
9he binding of coomassie brilliant blue to bo-ine serum albumin, @. )ohl
and A. )plittgerber, J. Chem. Educ. "# 6!6-6! "#99#%.
:-aluation of the Hill coefficient from )catchard and Clot* plots, A. )abouri
and A. Moosa-i-Mo-ahedi, Biochem. Educ. %% $-9 "#99%.
2luorescence measurements of ethidium binding to &7A, C. )trothkamp
and +. )trothkamp, J. Chem. Educ. 71 ,,-$# "#99%.
Analysis of receptor-ligand interactions, A. Attie and +. +aines, J. Chem.
Educ. 7% ##9-#6# "#99/%.
A ligand-binding study based on a protein assay method, H. 1hial et al., J.
Chem. Educ. 7% ,!-,$ "#99/%.
3inding of bilirubin to goat serum albumin: determination of binding
constants, ). 9ayyab and 4. 9ri-edi, Biochem. Educ. %& 9$-#55 "#99/%.

9he shapes of )catchard plots for systems .ith t.o sets of binding sites, A.
3ordbar et al., Biochem. Educ. %' #,6-#,/ "#99!%.
2luorescent measurement of 97) binding to calmodulin, @. 3uccigross et
al., J. Chem. Educ. 7& 6,/-6,$ "#99!%.
3inding of profla-in to chymotrypsin, H. Hendrickson, Biochem. Educ.
%7 ##$-#6# "#999%.
)tudy of protein-ligand 3inding by fluorescence, M. Moller and A.
&enicola, Biochem. Mol. Biol. Educ., &$ (59-(#6 "6556%.
4
9eaching receptor theory to biochemistry undergraduates, M. 3enore-
Parsons and C. )ufka, Biochem. Mol. Biol. Educ. &1 $/-96 "655(%.
:'uilibrium gel filtration chromatography for the measurement of protein-
ligand binding in the undergraduate biochemistry laboratory. &. 1raig, J. Chem.
Educ. #% 9!-9$ "655/%.
Auantitative *etermination of ;N.-li#an* bin*in# usin# Buorescence
spectroscopy, >. 'ealy, J. Chem. Educ. 84, 130/-130 (200!.
Molecular mo*els of ;.(C, 5. "oleman, J. Chem. Educ. 84, 130 (200!.
Stea*y-state Buorescence anisotropy to investi#ate Bavonoi*s bin*in# to
proteins, ". Cn#ersoll an* ". Strollo, J. Chem. Educ. 84, 1313-1317 (200!.
8< thermal meltin# curves of t9N.-(he in the presence of li#an*s, S. 1ir2
et al., J. Chem. Educ. 85, 7/-77 (2004
-pectroscopy
2luorescence 'uenching of albumin. A spectrofluorimetric experiment, M.
Montero et al. Biochem Educ. 1# 99-#5# "#995%.
?sing high field 7M+ to determine dehydrogenase stereospecificity .ith
respect to 7A&H, ). Mostad and A. 8lasfeld, J. Chem. Educ. 7$ /5-/5!
"#99(%.
7M+ titration used to obser-e specific proton dissociation in polyprotic
tripeptides, @. <arger, +. 7ieman, and A. 3ieber, J. Chem. Educ. 7' 6(-6/
"#99,%.
+esonance energy transfer in peptides: A biophysical laboratory experiment, A.
Hund*inski and 3. Anderson, J. Chem. Educ. 7" #!-#$"#999%.
Proton 7M+ )tudies of the conformation of an octapeptide, A. +ehart and @.
8erig, J. Chem. Educ. 77 $96-$9/ "6555%.
1arboxyfluorescin fluorescence experiments, ), Massou, +. Albigot, and
M. Prats, Biochem. Educ. %# #,#-#,( "6555%.
Protein tryptophan accessibility studied by fluorescence 'uenching, M.
Moller and A. &enicola, Biochem. Mol. Biol. Educ. &$ #,/-#,$ "6556%.
9he free energy of denaturation of lyso*yme: an undergraduate experiment
in biophysical chemistry, D. Curtin and @. =ee, Biochem. Mol. Biol. Educ.
&$ 6-6, "6556%.
5
=abeling of Proteins .ith 2luorescent Probes, 4. =e-i and 2. 2lecha,
Biochem. Mol. Biol. Educ. &1 (((-((! "655(%.
0n-estigation of model cell membranes .ith +aman spectroscopy, 7.
1raig, D. 2uchsman, and 7. =acuesta, J. Chem. Educ. #$ #6$6-#6$$
"655(%.
2undamentals of biomolecule analysis by electrospray ioni*ation mass
spectrometry. A. Deinecke and 4. +y*ho-, J. Chem. Educ. #% 99-#56.
. rapi* metho* for the *etermination of the percenta#e of aliphatic versus
vinyl hy*ro#en atoms an* levels of trans fatty aci*s in triacyl#lycerols, M.
Mosher et al., The Chemical Educator, 11, 1-3 (2007!.
Spectroscopic measurement of the re*o, potential of cytochrome c for the
un*er#ra*uate biochemistry laboratory, ;. "rai# an* >. Nichols, J. Chem.
Educ. 83,1325-132 (2007!.
.ccessible NM9 e,periments stu*yin# the hy*ro*ynamics of 15-N-
enriche* ubiquitin at lo$ @el*s, ). ?hompson an* ;. 9ovnya2, Biochem.
Mol. Biol. Educ. 35, /6-57 (200!.
Surface plasmon resonance label-free monitorin# of antibo*y anti#en
interactions in real time, .. 1ausaite et al., Biochem. Mol. Biol. Educ.
35, 5-73 (200!.
"omple,ation of copper (CC! ion $ith tetra#lycine as follo$e* by electronic
absorption spectroscopy, >. =arribba an* =. Micera, J. Chem. Educ. 84,
432-435 (200!.
Auantitative measurement of trans-fats by infrare* spectroscopy, >.
5al2er et al., J. Chem. Educ. 84, 1172-1175 (200!.
.bsorption an* scatterin# coeDcients- a biophysical-chemistry e,periment
usin# reBectance spectroscopy, =. "or*on an* M. )a#orio, J. Chem.
Educ. 84, 117-111 (200!.
Auantitative *etermination of ;N.-li#an* bin*in# usin# Buorescence
spectroscopy, >. 'ealy, J. Chem. Educ. 84, 130/-130 (200!.
Molecular mo*els of ;.(C, 5. "oleman, J. Chem. Educ. 84, 130 (200!.
Stea*y-state Buorescence anisotropy to investi#ate Bavonoi*s bin*in# to
proteins, ". Cn#ersoll an* ". Strollo, J. Chem. Educ. 84, 1313-1317 (200!.
6
. proEect-base* biochemistry laboratory promotin# the un*erstan*in# an*
uses of Buorescence spectroscopy in the stu*y of biomolecular structures
an* interactions, N. :riese an* '. %a2ubo$s2i, Biochem. Mol. Biol.
Educ. 35, 22-26 (200!.
)actate *ehy*ro#enase 2inetics an* inhibition usin# a microplate rea*er,
%. (o$ers et al., Biochem. Mol. Biol. Educ. 35, 24-262 (200!.
.n improve* metho* for stu*yin# the en&yme-cataly&e* o,i*ation of
#lucose usin# luminescent probes, 5. :are et al., J. Chem. Educ. 84,
1511-1515 (200!.
Cncorporatin# biolo#ical mass spectrometry into un*er#ra*uate teachin#
labs, (art C- i*entifyin# proteins base* on molecular mass, C. .rnquist an*
;. :eussman, J. Chem. Educ. 84, 161-16/ (200!.
)yso&yme thermal *enaturation an* self-interaction, %. Sch$inefus et al.,
J. Chem. Educ. 85, 11-120 (2004!.
0luorescence spectroscopy of t9N.-(he F base in the presence of M#(2G!
an* small li#an* molecules, S. 1ir2 et al., J. Chem. Educ. 85, 74-740 (2004!.
8sin# 09>? to measure the an#le at $hich a protein ben*s ;N., %. 1u#el,
Biochem. Mol. Biol. Educ. 36, 5/1-5/7 (2004!.
C*entifyin# #el-separate* proteins usin# in-#el *i#estion, mass spectrometry,
an* *atabase searchin#, ;. :eussman et al., Biochem. Mol. Biol. Educ. 37,
/6-55 (2006!.
II. Isolation and .nalysis o/ Biomolecules

.mino acids/peptides/proteins/anti0odies
Application of gel filtration for fractionation and molecular .eight determination
of proteins, >. Malhotra and A. Cumar, Biochem. Educ. 17 #$-#/5 "#9$9%.
A re-ie. of en*yme-immunoassay and a description of a competiti-e
en*yme-linked immunosorbent assay for the detection of immunoglobulin
concentrations, +. >ECennedy et al., Biochem. Educ. 1# #(!-#5 "#995%.
0solation of the acti-e site of cytochrome c by re-erse-phase high
performance li'uid chromatography, P. Cenigsberg et al. , J. Chem. Educ. "7
#,,-#,9 "#995%.
7
Destern blotting .ith a concana-alin A-horseradish conAugate, :. )nyder
and +. 2all, Biochem. Educ. 1# #,-#$ "#995%.
0nsulin: HP=1 mapping of protease digestion products, M. 4esting, J. Chem.
Educ. "# 9/$-9!5 "#99#%.
0solation and characteri*ation of bo-ine milk F-lactalbumin by 0MA1, +. 3oyer,
J. Chem. Educ. "# (5-(6 "#99#%, and Modern Experimental Biochemistry,
(
rd
ed. p. 6/,-6,$, "6555%, 3enAamin 1ummings ")an 2rancisco%.
0dentification of amino acids in unkno.n dipeptides: a deri-ati*ation .ith
9-fluorenylmethyl chloroformate and HP=1, 1. 1lapp et al., J. Chem. Edu.
"! A#66 "#996%.
&etermination of distances bet.een chromophores in proteins: a resonance-
energy transfer experiment, 8. 8on*ale*, Biochem. Educ. %%#/5-#/# "#99%.
A fast and inexpensi-e .estern blot for the undergraduate laboratory, ).
2arrell and =. 2arrell, J. Chem. Educ. 7& ,5-, "#99/%.
&eri-ation of the thermodynamic parameters in-ol-ed in the elucidation of
protein thermal profiles, A. )aarboury and A. Moosa-i-Mo-ahedi, Biochem.
Educ. %& #!-#!! "#99/%.
:xperimental determination of the free energy of unfolding of proteins,
). 9ayyab et al., Biochem. Educ. %& #!6-#!/ "#99/%.
?se of monoclonal antibodies in :=0)A assays, @. @ohnson, et al., Biochem.
Educ. %' /5-/6 "#99!%.
=abeling histidines in cytochrome c, A. 3onser and >. Moe, J. Chem. Educ.
7& ,9-,9, "#99!%.
0solation and purification of calmodulin from bo-ine brain, @. 3uccigross et al.,
J. Chem. Educ. 7& 6,(-6,! "#99!%.

Protein structure and chromatographic beha-ior: 9he separation and
characteri*ation of four proteins using gel filtration and ion-exchange
chromatography and gel electrophoresis, M. 1hakra-arthy, =. )nyder, 9.
4anyo, @. Holbrook, and H. @akubo.ski, J. Chem. Educ. 7& 6!$-6,6 "#99!%.
An experiment on the succinylation of .heat gluten and its conse'uences for
pH-solubility, &. 2ou'ues and M-1 +alet, Biochem. Educ. %' 6(6-6(( "#99!%.
Purification of horse heart cyt c by ion exchange, P. Mabrouk, J. Chem. Educ.
7& A#9 "#99!%.
8
Microburger biochemistry: extraction and spectral characteri*ation of
myoglobin from hamburger, ). 3ylkas and =. Andersson, J. Chem. Educ. 7'
6!-69 "#99,%.
An introduction to research in protein folding for undergraduates, 1.
@ones, J, Chem. Educ. 7' #(5!-#(#5 "#99,%.
)imulated .estern blot for the science curriculum, P. 1ummings, Biochem.
Educ. %( (9-5 "#99,%.
A simple immunoassay-based system capable of detecting antibody raised
against human 0g8, 8. Dalsh et al., Biochem. Educ. %" #/,-#!5 "#99$%.
:n*yme-linked antibodies: A laboratory introduction to the :=0)A, 8.
Anderson and =. Mc7ellis, J. Chem. Educ. 7( #6,/-#6,$ "#99$%.

0solation of gamma-globulin "0g8% from serum: a task that encourages
students to consolidate concepts on protein structure and properties, A.
Assis-Pandochi et al., Biochem. Educ. %" !(-!/ "#99$%.
3iochemical characteri*ation of transducin, the 8 protein of bo-ine retina,
@. Muschietti et al., Biochem. Educ. %" ,,-$# "#99$%.
0n-estigating lipoproteins, M. 8illett, Biochem. Educ. %7 /#-/ "#999%.
8reen 2luorescent Protein 0soforms: :xercises to integrate molecular biology,
biochemistry, and biophysical chemistry, 3. Hicks, J. Chem. Educ. 7" 59-
#! "#999%.

A con-enient and highly specific .estern blot experiment for introductory
biochemistry, 1. 2enk, ). 8rooms, and &. 8erbig, J. Chem. Educ. 77 (,(-
(,/ "6555%.
A simple method for immunodetection of membrane-associated proteins,
C. &aghastanli, +. 2erreira, 8. 9hedei, and P. 1iancaglini, Biochem. Mol.
Biol. %# 6/!-6!5 "6555%.
0dentification of serum glycoproteins by )&)-PA8: and .estern blotting,
+. 3oyer, Modern Experimental Biochemistry, (
rd
ed., p. (6#-((6 "6555%
3enAamin 1ummings ")an 2rancisco%.
A practical approach to the choice of a suitable detergent and optimal
conditions for solubili*ing a membrane protein, H. )antos and P. 1iancaglini,
Biochem. Educ. %# #,$-#$6 "6555%.
9
1omplete Analysis of a 3iologically Acti-e 9etrapeptide: A proAect utili*ing
thin-layer chromatography and tandem 'uadrupole mass spectrometry,
@. =e2e-re and &. &ods.orth, J. Chem. Educ. 77 /5(-/5/ "6555%.

A 3iochemical )tudy of 7onco-alent 2orces in Proteins using Phycocyanin
from Spirulina, 3. Heller and <. 8indt, J. Chem. Educ. 77 #/$-#!5 "6555%.
)tructural analysis of a dipeptide, +. 3oyer, Modern Experimental Bio-
chemistry, (
rd
ed. p. 66,-66, "6555%, 3enAamin 1ummings ")an 2rancisco%.
)tructural )tudies of Phycobiliproteins from Spirulina: 1ombining
spectroscopy, thermodynamics, and molecular modeling in an undergraduate
biochemistry experiment, A. 9aylor and ). 2eller, J. Chem. Educ. 7! #!,-
#,5 "6556%.
Assessment of the purification of a protein by ion exchange and gel
permeation chromatography, M. Pugh and :. )chult*, Biochem. Mol. Biol.
Educ. &$ #,9-#$( "6556%.
0dentifying a Protein by MA=&0-9>2 Mass )pectrometry, A. 1ounterman,
M. 9hompson, and &. 1lemmer, J. Chem. Educ. #$ #,,-#$5 "655(%.
Preparing undergraduates to participate in the post-genome era: A capstone
laboratory experience in proteomics, :. :berhardt, Biochem. Mol. Biol. Educ.
&1 56-59 "655(%.
3ringing proteomics into the lab, 2. 1ar-alho and M. 8illespie, Biochem. Mol.
Biol. Educ. &1 !-/# "655(%.
9eaching proteomics, =. Plesniak and :. 3ell, Biochem. Mol. Biol. Educ. &1
#6,-#(5 "655(%.
Purification of colored photosynthetic proteins for understanding protein
isolation principles, M. 3es, @. )ancho, M. Peleato, M. Medina, 1. 8ome*-
Moreno, and M. 2illat, Biochem. Mol. Biol. Educ. &1 ##9-#66 "655(%.
0solation of chicken immunoglobulins "0g<% from egg yolk, @. 8oldring and
9. 1oet*er, Biochem. Mol. Biol. Educ. &1 #$/-#$, "655(%.
)alt fractionation of plasma proteins: a procedure to teach principles of
protein chemistry, A. )padaro, A. Assis-Pandochi, <. =ucisano-4alim,
and ;. +othschild, Biochem. Mol. Biol. Educ. &1 69-6/6 "655(%.
"ytochrome c- . biochemistry laboratory course, %. <incent an* S. 5os2i,
J. Chem. Educ. 82, 1211-121/ (2005!.
10
Spectroscopic measurement of the re*o, potential of cytochrome c for the
un*er#ra*uate biochemistry laboratory, ;. "rai# an* >. Nichols, J. Chem.
Educ. 83,1325-132 (2007!.
Survey of biochemical separation techniques, M. Nilsson, J. Chem. Educ.
84,112-115 (200!.
.ccessible NM9 e,periments stu*yin# the hy*ro*ynamics of 15-N-
enriche* ubiquitin at lo$ @el*s, ). ?hompson an* ;. 9ovnya2, Biochem.
Mol. Biol. Educ. 35, /6-57 (200!.
Surface plasmon resonance label-free monitorin# of antibo*y anti#en
interactions in real time, .. 1ausaite et al., Biochem. Mol. Biol. Educ. 35,
5-73 (200!.
"hromato#raphic separation of t$o proteins, %. S&eberenyi, Biochem. Mol.
Biol. Educ. 35, 1-2 (200!.
5estern blot analysis to illustrate relative control levels of the lac an* ara
promoters in E. coli, :. Nielsen et al., Biochem. Mol. Biol. Educ. 35, 133-
13 (200!.
Stea*y-state Buorescence anisotropy to investi#ate Bavonoi*s bin*in# to
proteins, ". Cn#ersoll an* ". Strollo, J. Chem. Educ. 84, 1313-1317 (200!.
. proEect-base* biochemistry laboratory promotin# the un*erstan*in# an*
uses of Buorescence spectroscopy in the stu*y of biomolecular structures
an* interactions, N. :riese an* '. %a2ubo$s2i, Biochem. Mol. Biol.
Educ. 35, 22-26 (200!.
;etermination of the subunit molecular mass an* composition of alcohol
*ehy*ro#enase by S;S-(.=>, :. Nash, J. Chem. Educ. 84, 1504-1511
(200!.
Cncorporatin# biolo#ical mass spectrometry into un*er#ra*uate teachin#
labs, (art C- i*entifyin# proteins base* on molecular mass, C. .rnquist an*
;. :eussman, J. Chem. Educ. 84, 161-16/ (200!.
)yso&yme thermal *enaturation an* self-interaction, %. Sch$inefus et al.,
J. Chem. Educ. 85, 11-120 (2004!.
.n inte#rate* protein chemistry laboratory- "hlorophyll an* chlorophyllase
1. .r2us an* %. %e&, Biochem. Mol. Biol. Educ. 36, 125-124 (2004!.
11
?eachin# protein puri@cation an* characteri&ation techniques- . stu*ent-
initiate*, proEect-oriente* biochemistry laboratory course, =. Mac;onal*,
J. Chem. Educ. 85, 1250-1253 (2004!.
C*entifyin# #el-separate* proteins usin# in-#el *i#estion, mass spectrometry,
an* *atabase searchin#, ;. :eussman et al., Biochem. Mol. Biol. Educ. 37,
/6-55 (2006!.
Car0ohydrates
1hanges in carbohydrate content during fruit ripeningGa ne. approach of
teaching carbohydrate chemistry in biochemistry course, P. 1haimanee
and >. )untorn.at, Biochem. Educ. %% #5#-#56 "#99%.
8uidelines for determining the glycosylation characteristics of glycoconAugates
by simple procedures, @. 1abe*as, Biochem. Educ. %& #!$-#,5 "#99/%.
1hange in carbohydrate content during chickpea cooking: a ne. approach
of teaching of carbohydrate chemistry in biochemistry course, 4. Mulimani,
). 9hippes.amy and +amalingam, Biochem. Educ. %( -/ "#99,%.

1arbohydrate Analysis: 1an .e control the ripening of bananasH, ). &eal,
1. 2armer, and P. 1erpo-ic*, J. Chem. Educ. 7! ,9-$5 "6556%.
,ipids
0solation and spectrophotometric characteri*ation of photosynthetic
pigments, +. 3oyer, Biochem. Educ. 1# 65(-65! "#995%, and Modern
Experimental Biochemistry, (
rd
ed., p. (((-(, "6555% 3enAamin 1ummings.
")an 2rancisco%.
:rythrocyte storage as a model for lipid peroxidation studies. An experi-
mental assay for students of biochemistry and molecular biology, M.
=lanillo et al., Biochem. Educ. %% #/$-#!# "#99%.
Purification and characteri*ation of triacylglycerols in natural oils, +. 3oyer,
Modern Experimental Biochemistry, (
rd
ed. p. (5(-(65 "6555%, 3enAamin
1ummings ")an 2rancisco%.
&etermination of the 2atty Acid 1ontent of 3iological Membranes: A highly
-ersatile 81-M) :xperiment, :. )chult* and M. Pugh, J. Chem. Educ. 7#
9-9! "655#%.
12
&ifferential scanning calorimetric study of bilayer membrane phase
transitions, ). >hline, M. 1ampbell, M. 9urnbill, and ). Cohler, J. Chem.
Educ. 7# #6/#-#6/, "655#%.
An impro-ed method for the extraction and thin-layer chromatography of
chlorophyll a and b from spinach. H. Iuach, +. )teeper, and 8. 8riffin, J.
Chem. Educ. #1 ($/-($, "655%.
. rapi* metho* for the *etermination of the percenta#e of aliphatic versus
vinyl hy*ro#en atoms an* levels of trans fatty aci*s in triacyl#lycerols, M.
Mosher et al., The Chemical Educator, 11, 1-3 (2007!.
Auantitative measurement of trans-fats by infrare* spectroscopy, >.
5al2er et al., J. Chem. Educ. 84, 1172-1175 (200!.
Molecular mo*els of resveratrol, 5. "oleman, J. Chem. Educ. 84, 1171-
1172 (200!.
. proEect-base* biochemistry laboratory promotin# the un*erstan*in# an*
uses of Buorescence spectroscopy in the stu*y of biomolecular structures
an* interactions, N. :riese an* '. %a2ubo$s2i, Biochem. Mol. Biol.
Educ. 35, 22-26 (200!.
III. Isolation and Characteri1ation o/ En1ymes
:n*yme kineticsGthe steady state obser-ed, P. 3uckley et al., Biochem.
Educ. 1# #5#-#56 "#995%.
&etermining the conformation of a ligand bound to an en*yme: application
of 7M+ spectroscopy in drug design, &. 1raik et al., J. Chem. Educ. "#
6/$-6!5 "#99#%.
An 7M+ study of the stereochemistry of the fumarase-cataly*ed hydration
of fumaric acid, @. >lsen and +. >lsen, J. Chem. Educ. "# (!-(9 "#99#%.
4ersatile partial purification of glucose !-phosphate dehydrogenase by ion-
exchange chromatography, @. Celler et al., J. Chem. Educ. "# 6!/-6!$
"#99#%.
A biochemistry proAect to study mushroom tyrosinase, M. +odri'ue* and
D. 2lurkey, J. Chem. Educ ."! ,!,-,!9 "#996%.
Purification of lyso*yme by linear salt gradient and )&) gel electrophoresis,
M. Hurst et al., J. Chem. Educ. "! $/5-$/6 "#996%.
13
Probing denaturation by simultaneously monitoring residual en*yme acti-ity
and intrinsic fluorescence, 9. )il-erstein and =. 3lomberg, J. Chem. Educ.
"! $/6-$// "#996%.
Polyphenoloxidase: an en*yme .idespread in fruits, ). 8arcia and A.
3u*aleh, Biochem. Educ. %% #/6-#/( "#99%.
9he hydrolysis of p-nitrophenyl acetate: a -ersatile reaction to study en*yme
kinetics, @. Anderson, J. Chem. Educ. 71 ,#/-,#, "#99%.
9esting the F-amylase inhibitor of the common bean, @. Moreno et al.,
J. Chem. Educ. 71 (/5-(/6 "#99%.
A kinetic study of yeast alcohol dehydrogenase, +. ?techt, J. Chem. Educ.
71 (!-($ "#99%.
&esigning a coupled assay system for aspartate aminotransferase, P. Arnold
and 8. Parslo., Biochem. Educ. %& 5-# "#99/%.
A rapid and inexpensi-e procedure for the determination of proteolytic
acti-ity, ). 1astro and A. 1antera, Biochem. Educ. %& #-( "#99/%.
7ucleophilic and en*ymic catalysis of p-nitrophenylacetate hydrolysis, M.
Head et al., J. Chem. Educ. 7% #$-#$! "#99/%.
A practical experiment on en*yme immobili*ation and characteri*ation of the
immobili*ed deri-ati-es, A. ManAon et al., Biochem. Educ. %& 6#(-6#! "#99/%.
An experiment on the chemical modification of essential tryptophan residues of barley
J-amylase, 4. Mulimani and @. =alitha, Biochem. Educ. %& 6#$-6#9 "#99/%.
Cinetic study of the en*yme urease from Dolichos bilorus, C. 7ataraAan,
J. Chem. Educ. 7% //!-//$ "#99/%.
9eaching biochemistry to .ildlife management and oceanology students:
kinetics of =&H iso*ymes in brook charr, P. +ioux and P. 3lier, Biochem.
Educ. %& ($-(9 "#99/%.

An assay procedure for determining the rate of an en*yme reaction lacking
an optical signal: -alidity of coupled en*yme assays, >. Malhotra et al.,
Biochem. Educ. %' /!-/9 "#99!%.
;ymography of extracellular matrix proteases, A. Iuesada et al., Biochem.
Educ. %' #,5-#,# "#99!%.
14
An experiment on the denaturation of F-chymotrypsin by an anionic sur-
factant, sodium dodecyl sulfate ")&)%, 4. Mulimani and @. =alitha, Biochem.
Educ. %' /6-/ "#99!%.

A rapid and inexpensi-e procedure for the determination of amylase acti-ity,
4. Mulimani and @. =alitha, Biochem. Educ. %' 6(-6(/ "#99!%.
)pectrofluorimetric -isuali*ation of the progress of an en*ymatic reaction,
1. )aldanha and @. Martins-)il-a, Biochem. Educ. %' 6(/-6(! "#99!%.
+egiospecific ester hydrolysis by orange peel esterase, 9. 3ugg, A. =e.in,
and :. 1atlin, J. Chem. Educ. 7' #5/-#5, "#99,%.
0nhibition of cholinesterase by insecticides, =. =ai and @. Ho, Biochem. Educ.
%( 6(/-6(, "#99,%.
9he thermodynamic stability and catalytic acti-ity of yeast alcohol dehydrogen-
ase at different pH -alues, +. 9abor and @. =ad.ig, Biochem. Educ. %( #!9-
#,5 "#99,%.
A simple laboratory experiment for the teaching of the assay and kinetic
characteri*ation of en*ymes, =. 4icario, &. 1asti, and A. 0glesias, Biochem.
Educ. %( #5!-#59 "#99,%.
A no-el approach to practical en*ymology teaching: a conductimetric
in-estigation of arginase, inorganic pyrophosphatase, aliphatic esterase,
ornithine carbamyl transferase, and arginosuccinate lyase acti-ities from
mammalian li-er, A. =a.rence et al., Biochem. Educ. 26, /!-!6 "#99$%.
0mmobili*ed lactase in the biochemistry laboratory, M. Allison and 1. 3ering,
J. Chem. Educ. 7( #6,$-#6$# "#99$%.
Purification of bo-ine carbonic anhydrase by affinity chromatography, 1.
3ering and @. Cuhns, J. Chem. Educ. 7( #56#-#56/ "#99$%.
A #9-2 7M+ study of en*yme acti-ity, C. Peterman, C. =ent*, and @. &uncan,
J. Chem. Educ. 7( #6$(-#6$/ "#99$%.
9he competiti-e inhibition of yeast alcohol dehydrogenase by 6,6,6-trifluoro-
ethanol, +. 9abor, Biochem. Educ. %" 6(9-66 "#99$%.
Cinetics of Papain: An introductory biochemistry laboratory experiment, C.
1ornely, :. 1respo, M. :arley, +. Cloter, A. =e-es'ue,and M. Pickering,
J. Chem. Educ. 7" !-!! "#999%.
15
:n*ymic bro.ning in potatoes: a simple assay for a polyphenol oxidase
cataly*ed reaction, @. 3usch, Biochem. Educ. %7 #,#-#,( "#999%.
Cinetic analysis of tyrosinase, +. 3oyer, Modern Experimental Biochemistry
(
rd
ed. p.6,9-(56, "6555%, 3enAamin 1ummings ")an 2rancisco%.
1hemical cross-linking analysis of calcium-A9Pase from rabbit skeletal
muscle, 3. Murray and C. >hlendieck, Biochem. Educ. %# #-! "6555%.
Probing the acti-e site of yeast alcohol dehydrogenase through microscale
yeast-mediated reductions of acetophenone and acetylpyridines, M. =ee
et al. J. Chem. Educ. 77 (!(-(!! "6555%.
&emonstration of the principles of en*yme-cataly*ed reactions using
alkaline phosphatase, 7. Price and 3. 7e.man, Biochem. Mol. Biol.
Educ. %# 65,-6#5 "6555%.
9he determination of chitinase acti-ity of grapes, 7. 3yrne, M. &uxbury,
and 7. )harpe, Biochem. Mol. Biol. Educ. %! #-#! "655#%.
Aminotransferase from insect cells, H. :r.in, 8. de +idder, and P. 3arton,
Biochem. Mol. Biol. Educ. %! #9!-#9$ "655#%.
A no-el and inno-ati-e biochemistry laboratory: crystal gro.th of hen
egg .hite lyso*yme, J. Chem. Educ. 7! (!!-(!9 "6556%.
>ptimi*ation of kinetic parameters of en*ymes, +. Heinrich, :. Melende*-
He-ia, and H. 1abe*as, Biochem. Mol. Biol. Educ. &$ #$-#$$ "6556%.
Cinetic studies .ith alkaline phosphatase in the presence and absence of
inhibitors and di-alent cations, +. &ean, Biochem. Mol. Biol. Educ. &$ 5#-
5, "6556%.
(reparation, puri@cation, an* secon*ary structure *etermination of Bacillus
circulans ,ylanase. . mo*ular laboratory incorporatin# aspects of
molecular biolo#y, biochemistry, an* biophysical chemistry, S. 9usso an*
). =entile, J. Chem. Educ. 83, 1450-1452 (2007!.
'()" *etermination of ca+eine an* para,anthine in urine- .n assay for
cytochrome (/50 1.2 activity, ). 0ur#e an* 1. 0let2e, Biochem. Mol. Biol.
Educ. 35, 134-1// (200!.
8tili&ation of inte#rate* Michaelis-Menten equation to *etermine 2inetic
constants, 9. :e&erra an* .. ;ias, Biochem. Mol. Biol. Educ. 35, 1/5-150
(200!.
16
0rom e## to crystal- a practical on puri@cation, characteri&ation, an*
crystalli&ation of lyso&yme for bachelor stu*ents, <. Hlieric et al. Biochem.
Mol. Biol. Educ. 35, 240-247 (200!.
)actate *ehy*ro#enase 2inetics an* inhibition usin# a microplate rea*er,
%. (o$ers et al. Biochem. Mol. Biol. Educ. 35, 24-262 (200!.
.n improve* metho* for stu*yin# the en&yme-cataly&e* o,i*ation of
#lucose usin# luminescent probes, 5. :are et al., J. Chem. Educ. 84,
1511-1515 (200!.
Cmmobili&e* -#alactosi*ase in the biochemistry laboratory, <. Mulimani
an* 1. ;hananEay, J. Chem. Educ. 84, 16/-167 (200!.
?hermal an* chemical *enaturation of Bacillus circulans ,ylanase, ). =entile et
al., Biochem. Mol. Biol. Educ. 36, /24-/32 (2004!.
9e* sea$ee* en&yme-cataly&e* bromination of bromophenol re*- .n inquiry-
base* 2inetics laboratory e,periment for un*er#ra*uates, (. %ittam et al.,
Biochem.
Mol. Biol. Educ. 37, 66-105 (2006!.

I2. Meta0olism/3egulation/Transport
9he energetics of aerobic -ersus anaerobic respiration, 9. 1hampion and
+. )ch.en*, J. Chem. Educ. "7 /6$-/(5 "#995%.
?se of &1P0P in a colorimetric method to in-estigate electron transport in
crude heart mitochondrial extracts, A. Myers, Journal o Biol. Educ. %' #6(-
#6! "#995%.
Mitochondria from rat li-er: method for rapid preparation and study, 1. Heisler,
Biochem. Educ. 1! (/-($ "#99#%.
An experiment on glycogen biosynthesis in E. coli, A. =odeiro et al, Biochem.
Educ. %% 6#(-6# "#99%.
&emonstration of the regulation of phosphorylase by re-ersible
phosphorylation and allosteric effectors, +. 9oomik et al., Biochem. Educ. %%
65/-65, "#99%.
An experiment illustrating catabolite repression in yeast, D. 3aker, Biochem
17
Educ. %& 6#!-6#, "#99/%.
=ight-induced proton transport through chloroplast membranes, @. Ho and :.
Po, Biochem. Educ. %' #,9-#$5 "#99!%.
2ollo.ing glycolysis using #(-1 7M+, 9. Mega, 1. 1arlson, and &. 1leary,
J. Chem. Educ. 7' #,-#,! "#99,%.
)ome aspects of yeast anaerobic metabolism examined by the inhibition of
pyru-ate decarboxylase, :. Martin, J. Chem. Educ. 7( #6$#-#6$( "#99$%.
Photosynthesis and respiration in leaf slices, ). 3ro.n, Biochem. Educ. %"
#!-#!, "#99$%.
An experiment illustrating metabolic regulation in situ using digitonin permea-
boli*ed yeast cells, A. Ponces 2reire et al., Biochem. Educ. %" #!#-#!(
"#99$%.
A simple experiment demonstrating the allosteric regulation of yeast pyru-ate
kinase, +. 9aber, A. 1ampbell, and ). )pencer, Biochem. Educ. %" ,(-,!
"#99$%.
An in 4i-o #(-1 7M+ Analysis of the Anaerobic <east Metabolism of #-#(-1
8lucose, 3. 8iles, ;. Matsche, +. :geland, +. +eed, ). Morioka, and +.
9aber, J. Chem. Educ. 7" #/!-#/!! "#999%.
A simple experiment illustrating metabolic regulation: induction -ersus
repression of yeast F-glucosidase, 3. )tambuk, Biochem. Educ. %7 #,,-#$5
"#999%.

0solation, subfractionation, and en*ymatic analysis of beef heart mitochondria,
+. 3oyer, Modern Experimental Biochemistry, (
rd
ed. p. (/,-(,5 "6555%
3enAamin 1ummings ")an 2rancisco%.
9he excellence of turnip mitochondrial fractions, @. 4icente and 4. Madeira,
Biochem. Educ. %# #5-#5! "6555%.
A simple laboratory exercise illustrating acti-e transport in yeast cells,
3. )tambuk, Biochem. Mol. Biol. Educ. %# (#(-(#, "6555%.

Photoinduced proton transport through chloroplast membranes, +. 3oyer,
Modern Experimental Biochemistry, (
rd
ed. p. (/-(/! "6555%, 3enAamin
1ummings ")an 2rancisco%.
:xperimental treatment of the la.s of heterogeneous catalysis .ith
18
immobili*ed yeast cells, P. 8run.ald, Biochem. Educ. %# 9!-99 "6555%.
)tudying ion channels in undergraduate laboratories, A. 8arrill, Biochem.
Mol. Biol. Educ. %# (#$-(6# "6555%.
A laboratory experiment demonstrating the dynamic character of membranes,
M. Pugh and :. )chult*, Biochem. Mol. Biol. Educ. %# (66-(6! "6555%.
9he pentose phosphate path.ay in the yeasts Saccharomyces cere!isiae
and "loec#era apiculata, an exercise in comparati-e metabolism for food
and .ine science students, 1. )teel, P. 8rbin, and A. 7ichol, Biochem.
Mol. Biol. Educ. %! 6/-69 "655#%.
&esigner metabolic path.ays in the teaching laboratory, M. 8illett, Biochem.
Mol. Biol. Educ. %! #,-#/# "655#%.
Cinetic analysis of glucose-!-phosphatase: an in-estigati-e approach to
carbohydrate metabolism, M. Dallert, @. 2oster, &. )cholnick, A. >lmschenk,
3. Cuehn, and @. Pro-ost, Biochem. Mol. Biol. Educ. %! #99-65( "655#%.
7itrate reductase: A model system for the in-estigation of en*yme induction
in eukaryotes, 1. Pike, D. 1ohen, and @. Monroe, Biochem. Mol. Biol. Educ.
&$ ###-##! "6556%.
9ranscriptional and posttranslational regulation of a membrane nutrient
transporter, 3. )tambuk, Biochem. Mol. Biol. Educ. &$ ($$-(9( "6556%.
&iphenyl carba*ide restores electron transport in isolated, illuminated chloroplasts
after electron transport from .ater has been eliminated by mild heat treatment,
+. &ean and 9. Pocock, Biochem. Mol. Biol. Educ. &% ($#-($9 "655%.
)tudent-designed en*yme-linked metabolite assay kits. &. Hancock et al. Biochem.
Mol. Biol. Educ. &% (6!-((5 "655%.
(hotochemical o,i*ation of bilirubin to biliver*in, 5. "oleman, J. Chem.
Educ. 83, 1326-1330 (2007!.
Spectroscopic measurement of the re*o, potential of cytochrome c for the
un*er#ra*uate biochemistry laboratory, ;. "rai# an* >. Nichols, J. Chem.
Educ. 83, 1325-132 (2007!.
Solar irra*iation of bilirubin- .n e,periment in photochemical o,i*ation, ..
(illay an* 0. Salih, J. Chem. Educ. 83, 132-1326 (2007!.
5estern blot analysis to illustrate relative control levels of the lac an* ara
promoters in E. coli, :. Nielsen et al., Biochem. Mol. Biol. Educ. 35, 133-
13 (200!.
19
'()" *etermination of ca+eine an* para,anthine in urine- .n assay for
cytochrome (/50 1.2 activity, ). 0ur#e an* 1. 0let2e, Biochem. Mol. Biol.
Educ. 35, 134-1// (200!.
2. Clinical/4utritional
&etermination of urinary ammonium: a practical class experiment designed
to introduce medical students to mechanisms of extracellular and intra-
cellular pH homeostasis, 1. Marinho and @. 3arros, Biochem. Educ. %%
6#/-6#! "#99%.
A practical approach to chemical sensors through potentiometric transducers:
determination of urea in serum by means of a biosensor, :. Martine* and
). Alegret, J. Chem. Educ. 71 A!, "#99%.
9he role of molecular biology in characteri*ation of F-thalassaemia,
@. Ho.arth et al., Biochem. Educ. %' /9-!# "#99!%.
?se of blood-glucose test strips for introducing en*yme electrodes and modern
biosensors, @. Dang and 1. Macca, J. Chem. Educ. 7& ,9-,9, "#99!%.

9he analysis of ribofla-in in urine using fluorescence, @. Henderleiter and +.
Hyslop, J. Chem. Educ. 7& /!(-/!/ "#99!%.
Dorking .ith en*ymesG.here is lactose digestedH, ). Pope, 9. 9olleson,
+. Dilliams, +. ?nderhill, and +. &eal, J. Chem. Educ. 7( ,!#-,!6 "#99$%.
Measurement of cholesterol and -itamin 1 in biological samples, +. 3oyer,
Modern Experimental Biochemistry, (
rd
ed. p. (,#-($$ "6555%, 3enAamin
1ummings ")an 2rancisco%.
An experiment on the apoptosis induced by polyamine adducts produced
in the presence of serum amine oxidase, 0. 2aAardo, Biochem. Educ. %#
##5-##6 "6555%.

8lucocorticoid-induced apoptosis: a simple set of laboratory experiments,
8. 4icent, 8. =anu*a, &. +omero, =. &i 1roce, and A. Pecci, Biochem. Mol.
Biol. Educ. %# (5,-(#6 "6555%.
2rom gene to protein: Prostatic acid phosphatase: )tructure and expression of
gene and protein. P. =aidler et al., Biochem. Mol. Biol. Educ. &%5 55-59 "655%.
9esting for genetically modified foods using P1+. A. 9aylor and ). )aAan, J. Chem.
20
Educ. #% /9,-/99 "655/%.
(hotochemical o,i*ation of bilirubin to biliver*in, 5. "oleman, J. Chem.
Educ. 83, 1326-1330 (2007!.
Solar irra*iation of bilirubin- .n e,periment in photochemical o,i*ation, ..
(illay an* 0. Salih, J. Chem. Educ. 83, 132-1326 (2007!.
'()" *etermination of ca+eine an* para,anthine in urine- .n assay for
cytochrome (/50 1.2 activity, ). 0ur#e an* 1. 0let2e, Biochem. Mol. Biol.
Educ. 35, 134-1// (200!.
. laboratory e,ercise to *etermine human .:H bloo* type by noninvasive
metho*s, M. Martin an* S. ;et&el, Biochem. Mol. Biol. Educ. 36, 136-1/7
(2004!.
2I. Molecular Biology *nucleic acids cloning techniques+
An introduction to restriction mapping of &7A, 1. Hepfer and ). 9urchi,
Biochem. Educ. 17 $-/5 "#9$9%.
9he purification and translation of globin m+7A, :. Dolf, Biochem. Educ.
17 /-, "#9$9%.
+estriction en*yme mapping: a simple student practical, ). Higgins et al.,
Biochem. Educ. 1# #-#! "#995%.
Practical molecular biology for students: an integrated approach to teaching
basic techni'ues, 3. Hames et al., Biochem. Educ. 1# ##-# "#995%.
<ou too can be a molecular biologist: a basic cloning experiment for the
undergraduate biochemistry laboratory, ). 2arrell et al., J. Chem. Educ.
"# ,5,-,59 "#99#%.
2ield in-ersion agarose gel electrophoresis of &7A, &. Deller and P. 8ariepy,
J. Chem. Educ. "# $#-$( "#99#%.
&emonstration of &7A strand breakage induced by ultra-iolet light: an
experiment to sho. molecular e-ents in carcinogenesis, +. +usso and
@. +usso, J. Chem. Educ. 7$ ((5-((( "#99(%.
9he polymerase chain reaction, D. 9immer et al., and @. 4illalobos, J. Chem.
Educ. 7$ 6,(-6,, "#99(%.
P1+ amplification of &7A, &. Deller, J. Chem. Educ. 71 (5-(6 "#99%.
21
7on-radioacti-e detection of the Staphylococcus aureus protein A gene using
the polymerase chain reaction "P1+%, A. 1orbett et al, Biochem. Educ. %% /6-
// "#99%.
A fast restriction en*yme experiment for the undergraduate biochemistry
laboratory, ). 2arrell, J. Chem. Educ. 71 #59/-#59! "#99%.
A practical exercise in-ol-ing +7A isolation, transfer, and hybridi*ation,
M. Marano et al., Biochem. Educ. %% 65,-6#5 "#99%.
&7A composition analysis by nuclease digestion and HP=1, ). Dietstock,
J. Chem. Educ. 7% 9/5-9/6 "#99/%.
An analysis of the in-ol-ement of purine ribonucleotides in eukaryotic
protein synthesis, 9. =a.son, J. Chem. Educ. 7% #5-#5! "#99/%.
&etermination of the solution conformation of (E-a*ido-(Edeoxythymidine-
/E-monophosphate "A;9MP%, M. =ee, J. Chem. Educ. 7& #$-#$! "#99!%.
+esearch in undergraduate instruction: a biotech lab proAect for recombinant
protein expression in bacteria, M. 3rockman, A. >rdman and A. Malcolm, J.
Chem. Educ. 7& /6-/ "#99!%.
A laboratory class experiment illustrating basic principles of &7A cloning
and molecular biology techni'ues, =. Pere*-Pons and :. Iuerol, Biochem.
Educ. %' /-/! "#99!%.
+estriction mapping and gene analysis, M. )imsek, Biochem. Educ. %'
##,-##9 "#99!%.
A fast 19A3 method of human &7A isolation for polymerase chain reaction
applications, @. 9homas et al., Biochem. Educ. %( 6((-6(/ "#99,%.
Making your o.n gene library, @. Pere*->rtin et al., Biochem. Educ. %(
6(,-6# "#99,%.
Molecular genetic diagnosis by the polymerase chain reaction, A. 3eyer and
M. 4arsanyi, Biochem. Educ. %( #,5-#,( "#99,%.
0solation of nuclear, chloroplast, and mitochondrial &7A from plants, @. &ay
Biochem. Educ. %( #-( "#99,%.
A demonstration of genomic &7A profiling by +AP& analysis, @. Hammond
and 8. )pans.ick, Biochem. Educ. %( #59-### "#99,%.
22
&etection of non-3-&7A secondary structures by )# nuclease digestion,
M. del >lmo et al., J. Chem. Educ. 7( ,!6-,!, "#99$%.
)ynthesis and Purification of a Hammerhead +ibo*yme and a 2luorescein-
=abeled +7A )ubstrate. A 3iochemistry =aboratory: Part #, 1. 1ho. and
). )omne, J. Chem. Educ. 7" !$-!/# "#999%.
Monitoring Hammerhead +ibo*yme-1ataly*ed 1lea-age .ith a 2luorescein-
=abeled )ubstrate: :ffects of magnesium ions and antibiotic inhibitors, 1.
1ho., ). )omne, and 3. *llano-)otelo, J. Chem. Educ. 7" !/#-!/( "#999%.
A )tructure-2unction )tudy of +ecA, @. 8egner, 7. )pruill, and =. Plesniak,
J. Chem. Educ. 7" #/!6-#/! "#999%.
+7A isolation from plant tissues, M. 1laros and 2. 1ano-as, Biochem. Educ.
%7 ##5-##( "#999%.
An experiment illustrating &7A-protein interactions using nuclear extracts
from chicken erythrocytes, 3. 8arat, Biochem. Educ. %7 6(6-6(! "#999%.
1hromatin 0solation and &7A )e'uence Analysis in =arge ?ndergraduate
=aboratory )ections, A. Hagerman, J. Chem. Educ. 7" #6!-#69 "#999%.
A guided experimental approach to practical molecular pharmacology
teaching: demonstration of se'uence selecti-ity of &7A-binding drugs
by arrested polymerase chain reaction, +. 8ambari, Biochem. Educ.
%# #55-#5( "6555%.

&7A 9opology Analysis in the ?ndergraduate 3iochemistry =aboratory,
M. Ceck, J. Chem. Educ. 77 #,#-#, "6555%.
:xtraction and characteri*ation of bacterial &7A, +. 3oyer, Modern
Experimental Biochemistry, (
rd
ed. p. (99-# "6555%, 3enAamin 1ummings
")an 2rancisco%.
Plasmid &7A isolation and characteri*ation by electrophoresis, +. 3oyer,
Modern Experimental Biochemistry, (
rd
ed. p. #/-(5 "6555%, 3enAamin
1ummings ")an 2rancisco%.

9he action of restriction endonucleases on plasmid or -iral &7A, +. 3oyer,
Modern Experimental Biochemistry, (
rd
ed. p. (#-6 "6555%, 3enAamin
1ummings ")an 2rancisco%.
A laboratory exercise designed to teach medical students about re-erse-
transcription polymerase chain reaction, M. 2ay, 7. 1handar, and ).
23
4iselli, Biochem. Mol. Biol. Educ. %! 6(-6($ "655#%.
+estriction en*yme mapping of bacterial urease genes: using degenerate
primers to expand experimental outcomes, C. +eed, Biochem. Mol. Biol.
Educ. %! 6(9-6 "655#%.

3iocatalytic =actone 8eneration in 8enetically :ngineered E. coli and
0dentification of Products by 8as 1hromatography-Mass )pectroscopy,
1. )la.son, +. Potter, and @. )te.art, J. Chem. Educ. 7# #/((-#/(/,
"655#%.
0dentification of <east 4-A9Pase Mutants by Destern 3lots Analysis of
Dhole 1ell =ysates, C. Parra-3elky, J. Chem. Educ. 7! #($-#(/# "6556%.
&etection of genetically modified organisms in food by &7A extraction and
P1+ amplification, =. 9hion, 1. 4ossen, 3. 1ouderc, M. :rard, and 3.
1lemencon, Biochem. Mol. Biol. Educ. &$ /#-// "6556%.
0ntroducing students to &7A, H. )treicher and A. 3rodte, Biochem. Mol. Biol.
Educ. &$ #5-#5/ "6556%.
?se of chloroplast r+7A gene to introduce basic molecular biology techni'ues,
3. 7ielsen and ). :chols, Biochem. Mol. Biol. Educ. &$ 5$-#( "6556%.
0dentification of 2orensic )amples -ia Mitochondrial &7A in the ?ndergraduate
3iochemistry =aboratory, @. Millard and A. Pilon, J. Chem. Educ. #$ -!
"655(%.
A no-el, inexpensi-e, and highly sensiti-e experiment for demonstration of
&7A damage in human leukocytes by single cell electrophoresis "comet
assay%, 3. ManAunatha, 8. ManAunatha, 1. )arika, C. +oopa, and 8. 7aik,
Biochem. Mol. Biol. Educ. &1 (-(! "655(%.
1rime scene in-estigations, C. =ounsbury, Biochem. Mol. Biol. Educ. &1
(,-# "655(%.
A laboratory exercise in comparati-e &7A analysis, =. 3ro.n, Biochem.
Mol. Biol. Educ. &1 #,,-#,9 "655(%.
Photoinduced oxidati-e &7A damage re-ealed by an agarose gel nicking
assay, 4. )hafiro-ich, 1. )ingh, and 7. 8eacinto-, J. Chem. Educ. #$
#69,-#(55 "655(%.
>utcomes of a research-dri-en laboratory and literature course designed to
enhance undergraduate contributions to original research, M. +asche, Biochem.
Mol. Biol. Educ. &% #5#-#5, "655%.
24
(reparation, puri@cation, an* secon*ary structure *etermination of Bacillus
circulans ,ylanase. . mo*ular laboratory incorporatin# aspects of
molecular biolo#y, biochemistry, an* biophysical chemistry, S. 9usso an*
). =entile, J. Chem. Educ. 83, 1450-1452 (2007!.
5estern blot analysis to illustrate relative control levels of the lac an* ara
promoters in E. coli, :. Nielsen et al., Biochem. Mol. Biol. Educ. 35, 133-
3 (200!.
Molecular mo*els of ;N., 5. "oleman, J. Chem. Educ. 84, 406-410
(200!.
8sin# restriction mappin# to teach basic s2ills in the molecular biolo#y lab,
). 5alsh et al., Biochem. Mol. Biol. Educ. 35, 166-205 (200!.
Auantitative *etermination of ;N.-li#an* bin*in# usin# Buorescence
spectroscopy, >. 'ealy, J. Chem. Educ. 84, 130/-130 (200!.
Molecular mo*els of ;.(C, 5. "oleman, J. Chem. Educ. 84, 130 (200!.
. proEect-base* biochemistry laboratory promotin# the un*erstan*in# an*
uses of Buorescence spectroscopy in the stu*y of biomolecular structures
an* interactions, N. :riese an* '. %a2ubo$s2i, Biochem. Mol. Biol.
Educ. 35, 22-26 (200!.
(robin# chan#es in the conformation of t9N.-(he- an inte#rate* biochemistry
laboratory course, S. 1ir2 et al., J. Chem. Educ. 85, 777-7/ (2004!.
8< thermal meltin# curves of t9N.-(he in the presence of li#an*s, S. 1ir2
et al., J. Chem. Educ. 85, 7/-77 (2004!.
Metal-cataly&e* cleava#e of t9N.-(he, S. 1ir2 et al., J. Chem. Educ. 85,
77-74 (2004!.

0luorescence spectroscopy of t9N.-(he F base in the presence of M# (2G!
an* small li#an* molecules, S. 1ir2 et al., J. Chem. Educ. 85, 74-740 (2004!.
. laboratory e,ercise to *etermine human .:H bloo* type by noninvasive
metho*s, M. Martin an* S. ;et&el, Biochem. Mol. Biol. Educ. 36, 136-1/7
(2004!.
8sin# 09>? to measure the an#le at $hich a protein ben*s ;N., %. 1u#el,
Biochem. Mol. Biol. Educ. 36, 5/1-5/7 (2004!.
25
Caenorhabditis elegans as an un*er#ra*uate e*ucational tool for teachin#
9N.i, %. .n*ersen et al., Biochem. Mol. Biol. Educ. 36, /1-/2 (2004!.
. ne$ $ay to intro*uce microarray technolo#y in a lectureIlaboratory settin# by
stu*yin# the evolution of this mo*ern technolo#y, M. 9o$lan*-=ol*smith,
Biochem.
\Mol. Biol. Educ. 37, 3-/3 (2006!.
2II. Integrated Pro6ects
2ocusing on a single en*yme, @. Mark.ell, J. Chem. Educ. 7$ #5#$-#565 "#99(%.
An integrated approach to the undergraduate biochemistry laboratory, @.
Harman et al., J. Chem. Educ. 7% !#-!( "#99/%.
An integrated biochemistry laboratory including molecular modeling,
A. Dolfson, M. Hall, and 9. 3ranham J. Chem. Educ. 7& #56!-#569 "#99!%.
K+esearch in ?ndergraduate 0nstruction K, M. 3rockman, A. >rdman, and A.
1ampbell, J. Chem. Educ. 7& /6-/ "#99!%.
?tili*ing isolation, purification, and characteri*ation of en*ymes as proAect-
oriented labs for undergraduate biochemistry, ). &eal and M. Hurst, J.
Chem. Educ. 7' 6#-66 "#99,%.
A proAect-oriented biochemistry laboratory, P. 1raig, J. Chem. Educ. 7"
##(5-##(/ "#999%.
A single protein research integrated ad-anced biochemistry laboratory
course: spectroscopic determination of tyrosyl side chain pC, 3. 3lanchette
and 3. )ingh, Biochem. Educ. %# #5,-#59 "6555%.
0ntegrating computational chemistry into a proAect-oriented biochemistry
laboratory experience: a ne. t.ist on the lyso*yme experiment. +. Peterson,
@. 1ox, J. Chem. Educ. 7# #//#-#/// "655#%.
9he most proficient en*yme as the central theme in an integrated, research-
based biochemistry laboratory, @. )miley, Biochem. Mol. Biol. Educ. &$ /-
/5 "6556%.
&e-elopment of a biochemistry laboratory course .ith a proAect-oriented
goal, +. )tahelin, +. 2orslund, &. Dink, and D. 1ho, Biochem. Mol. Biol.
Educ. &1 #5!-##6 "655(%.
26
0solation, purification, and characteri*ation of bo-ine heart and yeast
cytochromes c: An integrated biochemistry laboratory experience,
3. Hunnes, Biochem. Mol. Biol. Educ. &1 66-6$ "655(%.
Preparing undergraduates to participate in the post-genome era: A
capstone laboratory experience in proteomics, :. :berhardt et al.,
Biochem. Mol. Biol. Educ. &1 56-59 "655(%.
An undergraduate biochemistry laboratory course .ith an emphasis on a
research experience, M. 1aspers and :. +oberts-Circhhoff, Biochem. Mol.
Biol. Educ. &1 (5(-(5, "655(%.
Ad-anced instrumentation proAects for first-year biochemistry laboratory,
M. Hall, 1. 8uth, ). Cohler, and A. Dolfson, Biochem. Mol. Biol. Educ.
&1 ##/-##$ "655(%.
8ene Amplification by P1+ and subcloning into a 82P-fusion plasmid expression
-ector as a molecular biology laboratory course, @. 3ornhorst, M. &eibel and
Mulnix, Biochem. Mol. Biol. Educ. &% #,(-#$6 "655%.
&e-elopment of green fluorescent protein-based laboratory curriculum,
P. =arkins and <. Hartberg, Biochem. Mol. Biol. Educ. && #-/ "655/%.
(reparation, puri@cation, an* secon*ary structure *etermination of Bacillus
circulans ,ylanase. . mo*ular laboratory incorporatin# aspects of molecular
biolo#y, biochemistry, an* biophysical chemistry, S. 9usso an* ). =entile,
J. Chem. Educ. 83, 1450-1452 (2007!.
5estern blot analysis to illustrate relative control levels of the lac an* ara
promoters in E. coli, :. Nielsen et al., Biochem. Mol. Biol. Educ. 35, 133-
13 (200!.
8sin# restriction mappin# to teach basic s2ills in the molecular biolo#y lab,
). 5alsh et al., Biochem. Mol. Biol. Educ. 35, 166-205 (200!.
. proEect-base* biochemistry laboratory promotin# the un*erstan*in# an*
uses of Buorescence spectroscopy in the stu*y of biomolecular structures
an* interactions, N. :riese an* '. %a2ubo$s2i, Biochem. Mol. Biol.
Educ. 35, 22-26 (200!.
. streamline* molecular mo*ule for un*er#ra*uate biochemistry labs, =. Muth
an* %. "hiha*e, Biochem. Mol. Biol. Educ. 36, 206-217 (2004!.
"lonin# yeast actin c;N. lea*s to an investi#ative approach for the molecular
27
biolo#y laboratory, M. :lac2 et al., Biochem. Mol. Biol. Educ. 36, 21-22/ (2004!.
(robin# chan#es in the conformation of t9N.-(he- an inte#rate* biochemistry
laboratory course, S. 1ir2 et al., J. Chem. Educ. 85, 777-7/ (2004!.
?eachin# protein puri@cation an* characteri&ation techniques- . stu*ent-
initiate*, proEect-oriente* biochemistry laboratory course, =. Mac;onal*,
J. Chem. Educ. 85, 1250-1253 (2004!.
?hermal an* chemical *enaturation of Bacillus circulans ,ylanase, ). =entile et
al., Biochem. Mol. Biol. Educ. 36, /24-/32 (2004!.
<ertical an* hori&ontal inte#ration of bioinformatics e*ucation, ). 0ur#e et al.,
Biochem. Mol. Biol. Educ. 37, 27-37 (2006!.
9Nase Hne #ene isolation, e,pression, an* aDnity puri@cation, ". :ailey,
Biochem. Mol. Biol. Educ. 37, //-/4 (2006!.
0rom #ene mutation to protein characteri&ation, ;. Mo+et, Biochem. Mol. Biol.
Educ. 37, 110-115. (2006!.
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