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FACULTY OF SCIENCE
UNIVERSITI TUNKU ABDUL RAHMAN
Jalan Universiti, Bandar Baru Barat, 31900 Kampar, Perak.
LABORATORY PRACTICAL REPORT
TITLE
Mitosis and Meiosis
PREPARED BY
NAME
DATE
LECTURER
1) 1205603
2) Ng Jia Li
2) 1307356
3) 1307452
th
8 August 2014
DR EE KAH YAW
CRITERIA
Introduction (10%)
Materials & Methods (15%)
Results (30%)
Discussion (30%)
Conclusion (10%)
References (5%)
TOTAL (100%)
Notes:
STUDENT ID.
SCORE
Result: Stages
Prophase
Description
The chromatin fibers become more tightly coiled,
condensing into more discrete chromosomes. The
UDBB1104 CELL BIOLOGY
nucleoli disappear. Each duplicated chromosomes
will appear as sister chromatids joined at the
centromeres. The mitotic spindle begins to form.
Prometaphase
Metaphase
Anaphase
Stages in Meoisis I
Phases
Descriptions
Prophase I
Metaphase I
Telophase I
States in Meiosis II
Phases
Description
Prophase II
Metaphase II
Telophase
Nuclei form, the chromosomes begin to decondense and cytokinesis occurs. The meiotic
division of one parent cell produces four
daughter cells, each with a haploid set of
(unduplicated) chromosomes. The four daughter
cells are genetically distinct from one another
and from the parent cell.
Discussion:
In this experiment, roop tips is used to observed under microscope. This is because
cell on root tips have faster growing rate compared to other part of onion. Beside that, the
onion root tips cell is easier to stain.
We use acetocarmine to stain chromosomes. The stained the chromosomes help to
distinguish from the other organelles and materials. The dye gives the chromosomes a red
color so that, we can observe the nucleus, duplication of DNA, and movement in cell cycle.
Staining is a simple preparatory technique that allows us to observe the otherwise
difficult to see nucleus of onion cells. There is no need to employ, possibly harmful, DNA
staining chemical.When you look at a stained cell you can easily see the components of the
cell. You should be able to see the cell wall, chromosomes, nucleus and mitochondria. In an
unstained cell, it would be very difficult to differentiate the components of the cell. For the
unstained cell, the nucleus was not stained. But when I got it stained we even saw the
ribosome and some other organelles. My results for observe the cell were more accurate and I
learn to try staining the slide whenever I can to see the cell and other things better on the
microscope. I think that when there is stain around the cell, then our eye can focus more on
the brighter side of the slide. This staining of onion cell makes a difference which allows us
Meiosis
A type of cellular
Mitosis
A process of asexual reproduction
separation of homologous
haploid cells.
Genetic diversity through
Type of Reproduction
Occurs in
sexual reproduction.
Sexual
Human, animals, plants,
Genetically
Crossing Over
fungi.
Different
Yes, mixing of chromosomes
Identical
No, crossing over cannot occur.
Pairing of Homologs
Number of Divisions
Number of Daughter
can occur.
Yes
2
4 haploid cells
No
1
2 diploid cells
cells produced
Chromosome Number
Steps
Reduced by half
Meiosis I:
Remain same
Prophase, Metaphase, Anaphase,
-Prophase I, Metaphase I,
Telophase.
Anaphase I, Telophase I
Meiosis II:
-Prophase II, Metaphase II,
Cytokinesis
Occurs in Telophase
Centromeres Split
Telophase II
The centromeres do not
anaphase.
cells.
Creates
Questions:
1. Interphase has sometimes been called a resting stage. Why is this inaccurate?
Interphase in the cell life cycle is a period where the cell carries out its typical
functions in preparation for cell division. It is inaccurate to say called a resting stage
because it is undergoing all of its life biochemical reactions except cell division which still
producing energy and using DNA replication to prepare for Mitosis. It is making proteins,
2. Some specialized cells such as neurons and red blood cells lose their ability to replicate
when they mature. Which phase of the cells cycle do you suspect is terminal for these cells?
Why?
In G1 the cell is just growing, and doing all the normal cell things. After being
checked a few times, it enters S phase, where the DNA is replicated. After that, and a few
more checkpoints, it moves to G2 phase, where it keeps growing and doing all the cell
processes normally. Finally, there is another checkpoint and then the cell jumps into M phase
which known as mitosis. The mitosis consists five part which is prophase, metaphase,
anaphase, telophase and cytokinesis. Then cytokinesis is the stage that cause the cell splits,
then will making two new ones both starting at G1.
Moreover, G0 is a special phase for specialized cells. The Cells enter G0 after
prompting from hormones and environmental factors, and before entering the S phase. Think
of it as an alternate route for the cell to follow other than the cell cycle circle. Once in G 0, the
cell ceases to divide. A vast majority of cells are in G 0, but can easily slip into G1 when they
need to replicated. When one cell dies, another slips back into the cell cycle to replicate and
then they both go into G0.
There are cells that won't ever divide again because they lack the ability to go back
into G1. For example, neurons and blood cells. Basically, they were divided specially from a
stem cell. One of the half kept most of everything that is needed to replicate again, and the
other went of and did its thing. When they die, they are dead. Blood cells themselves do not
replicated, but are continually made from adult stem cells in bone marrow. Neurons, on the
other hand, were made from embryonic stem cells and once dead are gone forever.
3. Why do you suppose cytokinesis generally occurs in the cells midplane?
Becker, W. M. (2009). The World of the Cell Seventh Edition. San Francisco: Pearson
Benjamin Cummings.
Kim, O. (2008). Staining of Onion Cell Nuclei. Retrieved August 2, 2014, from
MicrobeHunter Microscopy Magazine: http://www.microbehunter.com/staining-of-onioncell-nuclei/
Reece, J.B., Taylor, M.R., Simon, E.J., & Dickey, J.L. (2011). Campbell biology: concepts &
connections (7th ed.). Boston, MA: Pearson/Benjamin-Cummings.