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1) The study evaluated various substrates for cultivating the oyster mushroom Pleurotus pulmonarius in the Konkan region of Maharashtra, India.
2) The maximum biological efficiency was recorded on paddy straw (68.34%), followed by wheat straw (65.83%). Paddy straw supplemented with wheat bran produced the highest biological efficiency (80.44%).
3) Locally available substrates like paddy straw, arecanut husk, coconut husk, groundnut shells, sugarcane bagasse, banana pseudostem, and wheat straw were tested alone and in combination with rice bran and wheat bran supplements.
Исходное описание:
innovative technique for cultivation of pleurotus cultivation
1) The study evaluated various substrates for cultivating the oyster mushroom Pleurotus pulmonarius in the Konkan region of Maharashtra, India.
2) The maximum biological efficiency was recorded on paddy straw (68.34%), followed by wheat straw (65.83%). Paddy straw supplemented with wheat bran produced the highest biological efficiency (80.44%).
3) Locally available substrates like paddy straw, arecanut husk, coconut husk, groundnut shells, sugarcane bagasse, banana pseudostem, and wheat straw were tested alone and in combination with rice bran and wheat bran supplements.
1) The study evaluated various substrates for cultivating the oyster mushroom Pleurotus pulmonarius in the Konkan region of Maharashtra, India.
2) The maximum biological efficiency was recorded on paddy straw (68.34%), followed by wheat straw (65.83%). Paddy straw supplemented with wheat bran produced the highest biological efficiency (80.44%).
3) Locally available substrates like paddy straw, arecanut husk, coconut husk, groundnut shells, sugarcane bagasse, banana pseudostem, and wheat straw were tested alone and in combination with rice bran and wheat bran supplements.
1, 3 Dr. B.S. Konkan Krishi Vidyapeeth, Dapoli, Maharashtra 2 MPAU , Udaip!r, "a#asthan $orrespondin% A!thor& P. '. Borkar, Department o( Plant Patholo%y, $olle%e o( A%ri)!lt!re, Dapoli, Maharashtra *1+,12. -mail& pramod%./%mail.)om ABSTRACT Mild tropical humid climate persists in Konkan region throughout the year within a range of 23 3! " C# This climate is conduci$e for the commercial culti$ation of oyster mushroom# %ocally a$aila&le su&strates such as paddy straw' arecanut husk' coconut husk' &anana pseudostem' groundnut shells' sugarcane &agasse and wheat straw alone and in com&ination with rice &ran and wheat &ran were used# Ma(imum &iological efficiency of P. pulmonarius was recorded on paddy straw )*+#3",- followed &y wheat straw )*.# !3 ,-# /n case of supplemented su&strates' the ma(imum &iological efficiency of the mushroom was recorded on paddy straw supplemented with wheat &ran )0!#.",-# This was followed &y paddy straw supplemented with rice &ran )02#+3,- and wheat straw supplemented with wheat &ran )02#2+-# Key words1 Pleurotus pulmonarius, su&strates' Konkan' &iological efficiency /ntroduction he a.ility o( m!shrooms to %ro0 on a%ri)!lt!ral 0astes in less area makes an attra)ti1e proposition (or in)ome %eneration. he added ad1anta%e is in impro1ement in dietary 1al!es leadin% to sol!tion o( maln!trition, a.atement o( poll!tion and di1ersi(i)ation o( a%ri)!lt!re. he nat!ral ad1anta%es (or oyster m!shroom )!lti1ation in 2ndia are the presen)e o( seasonal 1ariations ena.lin% the )!lti1ation o( di((erent spe)ies !nder nat!ral )onditions in the (orm o( relay )roppin% in di((erent seasons and a.!ndan)e and easy as 0ell as )heap a1aila.ility o( 1ariety o( a%ro 0astes. Mild tropi)al h!mid )limate persists in Konkan re%ion thro!%ho!t the year 0ithin a ran%e o( 23 3 3+ 4 $. his )limate is )ond!)i1e (or the )ommer)ial )!lti1ation o( oyster m!shroom. Materials and methods1 Se1en lo)ally a1aila.le s!.strates 1i5. paddy stra0, are)an!t h!sk, %ro!ndn!t shells, s!%ar)ane .a%asse, )o)on!t h!sk, .anana pse!do stem, and 0heat stra0 0ere !sed to assess the .iolo%i)al e((i)ien)y o( P. pulmonarius on these s!.strates. Paddy stra0, 0heat stra0 and s!%ar)ane .a%asse 0ere )!t into +36)m pie)es. Are)a n!t h!sk, )o)on!t h!sk and %ro!ndn!t shells 0ere )r!shed 0ith a )ha(( )!tter. 7ne k% 8!antity o( ea)h s!.strate 0as 0ei%hed separately on an ele)troni) .alan)e. All the s!.strates 0ere soaked o1erni%ht in (resh tap 0ater. -9)ess 0ater in the s!.strates 0as drained o(( .y pla)in% the s!.strates on a )lean, )emented plat(orm 0ith desira.le slope. All the s!.strates 0ere immersed in hot 0ater :643;4< $= (or 2 hrs. A(ter 2 hrs the e9)ess 0ater 0as drained o(( and the s!.strates 0ere allo0ed to )ool at room temperat!re. he moist!re )ontent o( the hot 0ater treated s!.strates 0as aro!nd ,4 to ,2 per )ent. he s!.strates 0ere then spa0ned / 2 per )ent on 0et 0ei%ht .asis and (illed in polypropylene .a%s o( si5e, +3 > 3+ )m so as to make )ylindri)al m!shroom .eds. hree repli)ations 0ere maintained (or ea)h s!.strate. hese .a%s 0ere then per(orated .y makin% a.o!t 2+334 pinholes on all sides in)l!din% top and .ottom. M!shroom .eds th!s prepared 0ere then trans(erred to iron shel1es in spa0n r!n room. $omplete darkness and 2+?2 4 $ temperat!re 0as maintained in this room till )ompletion o( my)elial r!n. Beds 0ith )ompletely )oloni5ed s!.strate 0ere trans(erred to the han%in% .am.oo shel1es in )roppin% room and then the plasti) )o1er 0as remo1ed. he h!midity in the )roppin% room 0as maintained 0ithin a ran%e o( 6436+ per )ent 0ith the help o( mist .lo0ers 0hi)h 0ere r!n (or +314 min!tes, *3+ times a day. he e9periment 0as laid in $ompletely "andomi5ed Desi%n. hree repli)ations 0ere maintained per treatment. 2n )ase o( s!pplemented s!.strates, ea)h s!.strate 0as separately s!pplemented 0ith 4.+ per )ent ri)e .ran and 0heat .ran on 0et 0ei%ht .asis o( the s!.strate. he 0ei%hed 8!antities o( s!pplements 0ere mi9ed thoro!%hly, 0ith the s!.strate. hree repli)ations 0ere maintained per treatment in $"D. Results and 2iscussion1 3ffect of Su&strates on &iological efficiency of P. pulmonarius 2t is o.ser1ed (rom a.le 1, that the minim!m )oloni5ation period 0as re)orded in .anana pse!dostem s!.strate :1* days= (ollo0ed .y paddy stra0 : 24 days=and 0heat stra0 :21=respe)ti1ely. hey 0ere (ollo0ed .y s!%ar)ane .a%asse :22 days=, are)a n!t h!sk and %ro!ndn!t shells :2@ days=, and )o)on!t h!sk:3+ days=. -arly emer%en)e o( pin heads 0as re)orded in paddy stra0 and 0heat stra0 :* days= (ollo0ed .y are)an!t h!sk, %ro!ndn!t shells, s!%ar)ane .a%asse and .anana pse!dostem :@ days=. Delayed pin head (ormation 0as re)orded only in )o)on!t h!sk 0here pin heads appeared +4 days a(ter spa0nin%. 2n all the s!.strates, ma9im!m yield 0as o.tained (rom the (irst har1est. he loose te9t!re o( .anana pse!dostem mi%ht .e the reason (or speedy )oloni5ation. So(tenin% o( the s!.strate 0as o.ser1ed in all the other s!.strates a(ter soakin% in )old 0ater (ollo0ed .y hot 0ater treatment .!t the )o)on!t h!sk (i.res remained hard and to!%h. his may .e d!e the presen)e o( more amo!nt o( li%nin in )o)on!t (i.res. he Pleurotus spe)ies are a.le to %ro0 on a 0ide spe)tr!m o( lin%o3)ell!losi) 0aste material d!e to their a.ility to se)rete an array o( de%radin% en5ymes. he a)ti1ity these en5ymes 1ary a))ordin% to the )omposition o( the s!.strate and the spe)ies or strain o( the m!shroom :Perisamy and Aatara#an, 244*=. Ma9im!m .iolo%i)al e((i)ien)y :,@.34 B= o( P. pulmonarius 0as re)orded on paddy (ollo0ed .y 0heat stra0 : ,*.+3=.hese res!lts are in )on(ormity 0ith those reported .y earlier 0orkers :Ca5arika,1;;6,Dhan% et al., 2442 and Kimen#! et al. 244;=. 'eetha and Si1aprakasam :1;;6= reported that the paddy stra0 !sed (or )!lti1ation o( Pleurotus spe)ies sho0ed hi%her red!)tion in )ell!lose )ontent indi)atin% the pre(eren)e o( the m!shrooms to the s!.strates ri)h in )ell!lose. Both paddy and 0heat stra0 )ontain %ood amo!nt o( )ell!lose .!t perhaps the amo!nt li%nin in 0heat stra0 is more than that in paddy stra0. he reason (or lo0 .iolo%i)al e((i)ien)y in are)a n!t s!.strate may .e attri.!ted to the li%nin )ontent o( the s!.strate. ho!%h the .iolo%i)al e((i)ien)y 0as less as )ompared to stra0 s!.strates, this s!.strate needs to tried more n!m.er o( times (or this m!shroom as the .iolo%i)al e((i)ien)y is nearly +4 per )ent. 'ro!ndn!t shell s!.strate 0as the poorest per(ormer amon% the , s!.strates !nder st!dy. 2t re)orded only 1*.*, per )ent .iolo%i)al e((i)ien)y. Ci%h moist!re holdin% )apa)ity o( this s!.strate res!lted in less a1aila.ility o( (ree air in the s!.strate d!rin% spa0n r!n. his !ltimately had ad1erse e((e)t on s!.strate )oloni5ation and (r!it .ody (ormation. he present (indin%s are in )on)!rren)e 0ith the res!lts o( Mane et al. :244,= and Veena and Sa1al%i :1;;1=. he .iolo%i)al e((i)ien)y in s!%ar)ane .a%asse 0as nearly si9ty per )ent and it pro1ed to .e a .etter s!.strate (or )!lti1ation o( the m!shroom !nder st!dy. 28!.al et al., 244+, also reported similar res!lts. $o)on!t h!sk 0as si%ni(i)antly s!perior to %ro!ndn!t shells .!t in(erior to other s!.strates. Perisamy and Aatara#an: 244*= reported that some strains o( P. djamor 0ere )a.le o( de%radin% the li%nin polymers in )o)on!t )oir and there(ore re)orded .etter yield on )o)on!t )oir pith s!.strate 0hile others (ailed to do so. Banana pse!dostem ranked (i(th in terms o( .iolo%i)al e((i)ien)y. his s!.strate )ontains more amo!nt 0ater than dry matter. he lo0 yield on this s!.strate may .e attri.!ted to ma9im!m !tili5ation o( a1aila.le n!trients d!rin% )oloni5ation. 3ffect of su&strate supplementation on &iological efficiency of P. pulmonarius he ma9im!m .iolo%i)al e((i)ien)y o( the m!shroom 0as re)orded on paddy stra0 s!pplemented 0ith 0heat .ran :6+.*4B= 0hi)h 0as (ollo0ed .y paddy stra0 s!pplemented 0ith ri)e .ran :62.@3B= and 0heat stra0 s!pplemented 0ith 0heat .ran :62.2@=. Eatter t0o treatments 0ere statisti)ally at par 0ith ea)h other. Amon% the remainin% treatments, in treatment the 13 ,
0here 0heat stra0 0as s!pplemented 0ith ri)e .ran, the .iolo%i)al e((i)ien)y 0as nearly 64 per )ent. his 0as (ollo0ed .y s!%ar)ane .a%asse. he .iolo%i)al e((i)ien)y on s!%ar)ane .a%asse s!pplemented 0ith 0heat .ran and ri)e .ran 0as @3.43 and @1.4@ respe)ti1ely. Both these treatments 0ere statisti)ally at par 0ith ea)h other. he .iolo%i)al e((i)ien)y o( are)an!t h!sk 0ith 0heat .ran s!pplementation 0as +1.,4 per )ent and 0ith ri)e .ran it 0as *;.23 per )ent and the di((eren)e amon% these t0o treatments 0as statisti)ally si%ni(i)ant. Biolo%i)al e((i)ien)y on .anana pse!dostem s!pplemented 0ith 0heat .ran and ri)e .ran 0as 3,.64 and 3,.,4 per )ent respe)ti1ely and .oth these treatments 0ere statisti)ally at par. Moreo1er, the .iolo%i)al e((i)ien)y on )o)on!t h!sk s!pplemented 0ith .oth the s!pplements 0as statisti)ally at par. S!pplementation o( %ro!nd n!t shells 0ith .oth the s!pplements did not yield m!shrooms. his indi)ated that the s!pplementation 0as not e((e)ti1e in )ase o( this s!.strate. Se1en o( the 1* treatments, re)orded more than +4 per )ent .iolo%i)al e((i)ien)y and 0heat .ran s!pplementation re)orded hi%her yield than ri)e .ran. ho!%h, .oth s!pplements 0ere ine((e)ti1e 0ith %ro!ndn!t shell s!.strate. $oloni5ation period in all the s!pplemented s!.strates 0as less as )ompared to that re8!ired to )oloni5e the same s!.strate 0itho!t s!pplementation. Biolo%i)al e((i)ien)y on all the s!pplanted s!.strates e9)ept %ro!ndn!t shells 0as more than on non3s!pplemented s!.strates. Ma9im!m .iolo%i)al e((i)ien)y 0as re)orded in paddy stra0 s!pplemented 0ith 0heat .ran (ollo0ed .y paddy stra0 s!pplemented 0ith ri)e .ran and 0heat stra0 s!pplemented 0ith 0heat .ran. Similar res!lts 0ere reported .y many 0orkers :Khare et al., 244,F "!h!l Amin et al., 244, and Patil et al., 2414=. 'ro!nd n!t shell s!.strate retains more amo!nt o( moist!re 0hi)h res!lts in poor my)elial r!n. D!e to in)orporation o( s!pplements in this s!.strate, the .ed 0as so )ompa)t that there 0as 1ery little s)ope (or proli(eration o( m!shroom my)eli!m. his )ondition did not allo0 the primary my)eli!m to (orm tertiary my)eli!m 0hi)h a%%re%ates to (orm (r!it .odies o( m!shroom (!n%i. Biolo%i)al e((i)ien)y in paddy stra0 s!pplemented 0ith 0heat .ran 0as nearly @.+ per )ent more than that in sole paddy stra0. his indi)ates that protein ri)h s!pplements s!)h as 0heat and ri)e .ran ha1e positi1e e((e)t on )oloni5ation and .iolo%i)al e((i)ien)y o( oyster m!shrooms. 2t )an .e )on)l!ded on the .asis o( the res!lts o( present st!dy that, in)orporation proper s!pplements not only red!)e the )oloni5ation period .!t also in)reases the .iolo%i)al e((i)ien)y o( P. pulmonarius Ta&le 41 3ffect of Su&strates on &iological efficiency of P. pulmonarius. Tr# 5o# Treatments Spawn run 6eriod )days- 6inhead 7ormation )days- A$erage 8ield )g- B#3# ),- T 4 6addy straw 24 * ,@3.44 ,@.34 T 2 Arecanut 9usk 2@ @ *,@.33 *,.@3 T 3 :roundnut shells 2@ @ 1**.@, 1*.*, T . Sugarcane &agasse 22 @ +;3.33 +;.33 T ! Coconut husk 3+ 1+ 24;.@, 24.;, T + Banana pseudostem 1* @ 3,*.33 3,.*3 T * ;heat straw 21 * ,*+.33 ,*.+3 C2 at ! , 33#<. C2 at 4, .*#44 C= , .#4"
Ta&le 21 3ffect of su&strate supplementation on &iological efficiency of P. pulmonarius Tr# 5o# Treatments Spawn run 6eriod )days- 6inhead 7ormation )days- A$erage 8ield )g- B#3# ),- T 4 6> RB 1, + 62@.33 62.@3 T 2 6> ;B 1@ + 6+*.44 6+.*4 T 3 A> RB 22 , *;2.33 *;.23 T . A> ;B 24 + +1,.44 +1.,4 T ! :> RB 2* 3 4 4 T + :> ;B 2* 3 4 4 T * S> RB 24 , @14.@, @1.4, T 0 S> ;B 1; @ @34.33 @3.43 T < C> RB 31 13 214.44 21.44 T 4" C> ;B 34 12 211.44 21.14 T 44 B> RB 11 + 3,,.44 3,.,4 T 42 B> ;B 11 * 3,6.44 3,.64 T 43 ; > RB 1, * ,;;.@, ,;.;, T 4. ;> ;B 1, + 622.@, 62.2, C2 at !, 4.#4+ C2 at 4 , 4<#44 C= 4#*+
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The Use of Alum, Ferric Chloride and Ferrous Sulphate As Coagulants in Removing Suspended Solids, Colour and COD From Semi-Aerobic Landfill Leachate at Controlled PH