Process Biochemistry 40 (2005) 989995

Review
Bioreactor performance in anaerobic digestion
of fruit and vegetable wastes
H. Bouallagui
a,b,
, Y. Touhami
a
, R. Ben Cheikh
b
, M. Hamdi
a
a
UR-Procds Microbiologiques et Alimentaires, Institut National des Sciences Appliques et de Technologie (INSAT),
B.P. 676, 1080 Tunis, Tunisia
b
Ecole Nationale dIngnieurs de Tunis (ENIT), B.P. 37, 1002 Tunis, Tunisia
Received 2 December 2003; received in revised form 16 March 2004; accepted 28 March 2004
Abstract
This work reviews the potential of anaerobic digestion for material recovery and energy production from fruit and vegetable wastes (FVW).
These wastes contain 818% total solids (TS), with a total volatile solids (VS) content of 8692%. The organic fraction includes about 75%
easy biodegradable matter (sugars and hemicellulose), 9% cellulose and 5% lignin. Anaerobic digestion of FVW was studied under different
operating conditions using different types of bioreactors. It permits the conversion of 7095% of organic matter to methane, with a volumetric
organic loading rate (OLR) o f 16.8 g versatile solids (VS)/l day. A major limitation of anaerobic digestion of FVW is a rapid acidication
of these wastes decreasing the pH in the reactor, and a larger volatile fatty acids production (VFA), which stress and inhibit the activity of
methanogenic bacteria. Continuous two-phase systems appear as more highly efcient technologies for anaerobic digestion of FVW. Their
greatest advantage lies in the buffering of the organic loading rate taking place in the rst stage, allowing a more constant feeding rate of the
methanogenic second stage. Using a two-stage system involving a thermophilic liquefaction reactor and a mesophilic anaerobic lter, over
95% volatile solids were converted to methane at a volumetric loading rate of 5.65 g VS/l d. The average methane production yield was about
420 l/kg added VS.
2004 Elsevier Ltd. All rights reserved.
Keywords: Fruit and vegetable wastes; Anaerobic digestion; Limitation step; Bioreactors performance
1. Introduction
Fruit and vegetable wastes (FVW) are produced in large
quantities in markets, and constitute a source of nuisance
in municipal landlls because of their high biodegradabil-
ity [1,2]. In the central distribution market for food (meat,
sh, fruit, and vegetables) Mercabarna (Barcelona), the to-
tal amount of wastes coming from fruit and vegetables is
around 90 tonnes per day during 250 days per year [3]. The
whole production of FVW collected from the market of Tu-
nis (Tunisia) has been measured and estimated to be 180
tons per month [4]. In India, FVW constitute about 5.6 mil-
lion tonnes annually and currently these wastes are disposed
by dumping on the outskirts of cities [5].
The most promising alternative to incinerating and com-
posting these wastes is to digest its organic matter using the

Corresponding author. Tel.: +216 22 524 406; fax: +216 71 704 329.
E-mail address: hassibbouallagui@yahoo.fr (H. Bouallagui).
anaerobic digestion [4,6]. The main advantage of this pro-
cess is the production of biogas, which can be used to pro-
duce electricity [3,7,8]. A valuable efuent is also obtained,
which eventually can be used as an excellent soil condi-
tioner after minor treatments [9,10]. High organic loading
rates (OLR) and low sludge production are among the many
advantages anaerobic process exhibit over other biological
unit operations [11,12].
The successful application of anaerobic technology to
the treatment of solid wastes is critically dependent on the
development and use of high rate anaerobic bioreactors
[13,14]. The reactor design has a strong effect on digester
performance [15]. In recent years, a number of novel re-
actor designs have been adapted and developed allowing a
signicantly higher rate of reaction per unit volume of re-
actor [16,17]. Different anaerobic processes, such as batch,
continuous one-stage, and continuous two-stage systems,
with a variety of methanizers like, continuously stirred
tank reactor (CSTR), tubular reactor, anaerobic sequencing
batch reactor (ASBR), upow anaerobic sludge blanket
0032-9592/$ see front matter 2004 Elsevier Ltd. All rights reserved.
doi:10.1016/j.procbio.2004.03.007
990 H. Bouallagui et al. / Process Biochemistry 40 (2005) 989995
(UASB) and anaerobic lters have been applied to FVW
treatment. These processes differ especially in the way
the microorganisms are retained in the bioreactor, and the
separation between the acidogenic and the methanogenic
bacteria which reduce the anaerobic digestion limitations.
Methanogenic bacteria may have long mass doubling times
in anaerobic reactors and this makes it very difcult to
obtain fast acting reactors without retaining most of the
biomass normally washed out with the efuent [18,19].
The aim of this paper was to review the energetic potential
of FVWand to examine the performance of several groups of
anaerobic bioreactors used for anaerobic digestion of these
wastes.
2. Characteristics of FVW and anaerobic
digestion limitations
The putrescible FVW used in overall reported studies
were collected from food markets and Table 1 shows the
most important constituents of FVW in three works where
anaerobic digestion was operated [1,20,21]. The total initial
solid concentration of FVW is between 8 and 18%, with
a total volatile solids (VS) content of about 87%. The or-
ganic fraction includes about 75% sugars and hemicellulose,
9% cellulose and 5% lignin [20]. The easy biodegradable
organic matter content of FVW (75%) with high moisture
facilitates their biological treatment and shows the trend of
these wastes for anaerobic digestion [1,21]. However, com-
plex vegetable processing efuent, such as olive mill wastes
containing large amounts of phenolic and non-biodegradable
compounds are resistant to biological degradation [22]. Aer-
obic processes are not favoured for FVW treatment because
they require preliminary treatment to minimise the organic
loading rate [23]. The COD/N ratio of FVW is balanced,
being around 100/4 and therefore, no nitrogen was added to
the reactors. In fact the optimum C:N ratio for microbial ac-
tivity involved in bioconversion of vegetable biomasses to
methane is 100128:4 [24].
Before being loaded to the reactors, FVW must undergo
some pre-treatments [5,9]. They were shredded to small
Table 1
Composition of different fruit and vegetable wastes
Wastes (g/kg) Potato peelings Salad waste Green peas and carrots Mixture of FVW Mixture of FVW
Total solids 119.2 79.4 179.4 90.4 84.4
Volatile solids 105.5 72.1 171 82.9 77.5
Total COD 126 97.8 185 104.5
Particulate COD 80.6 39.3 123.9
Total suspended solids 80 39 145 58.6
Total Kjeldhal Nitrogen 2 2.7
Cellulose 12.9 13.5 16.1 9.2
Sugars and hemicellulose 62
Lignin 4.5
References [21] [21] [21] [20] [1]
particles and homogenized to facilate digestion. They were
also diluted to decrease the concentration of organic matter
and then to operate the reactors with optimal organic load-
ing rate [3,4]. Due to the lower pH of FVW, some authors
buffered these waste by the addition of sodium hydroxide
solutions [5,6]. Without any regulation, the pH quickly de-
creased and tended to inhibit the methanogenic bacteria [20].
Converti et al. pre-treated organic matter of FVW at high
temperature to improve the efciency of their anaerobic di-
gestion [9], while Srilatha et al. pre-treated orange process-
ing waste by solid state fermentation using selected strains
of Sporotrichum, Aspergillus, Fusarium, and Penicillium to
improve biogas and methane productivity at higher OLR [5].
The biomethanation of FVW is accomplished by a se-
ries of biochemical transformations, which can be roughly
separated into four metabolic stages [25,23] (Fig. 1). First,
particulate organic materials of FVW like cellulose, hemi-
cellulose, pectin, and lignin, must undergo liquefaction by
extracellular enzymes before being taken up by acidogenic
bacteria [26]. The rate of hydrolysis is a function of factors,
such as pH, temperature, composition, and particle size of
the substrate and high concentrations of intermediate prod-
ucts [27,28]. After that, soluble organic components includ-
ing the products of hydrolysis are converted into organic
acids, alcohols, hydrogen, and carbon dioxide by acidogens.
The products of the acidogenesis are then converted into
acetic acid, hydrogen, and carbon dioxide. Finally, methane
is produced by methanogenic bacteria from acetic acid, hy-
drogen, and carbon dioxide as well as directly from other
substrates of which formic acid and methanol are the most
important [28].
In general, hydrolysis is the rate limiting step if the sub-
strate is in particulate form [29,30]. However, the anaerobic
degradation of cellulose-poor wastes like FVW is limited
by methanogenesis rather than by the hydrolysis [31,32].
These wastes, are very rapidly acidied to volatile fatty
acids (VFA) and tend to inhibit methanogenesis when the
feedstock is not adequately buffered [23]. In one-stage sys-
tems, all these reactions take place simultaneously in a sin-
gle reactor, while in two-or multistage systems, the reac-
tions take place sequentially in at least two reactors. In a
H. Bouallagui et al. / Process Biochemistry 40 (2005) 989995 991
Fig. 1. Reactions scheme for anaerobic digestion of particulate organic material of FVW [3,20,25,28,49].
well-balanced anaerobic digestion process, all products of
a previous metabolic stage are converted into the next one
without signicant build up of intermediate products [33].
The overall result is a nearly complete conversion of the
anaerobically biodegradable organic material into end prod-
ucts like methane, carbon dioxide, hydrogen sulphide, and
ammonia.
3. Anaerobic bioreactors used for FVW biomethanation
3.1. Batch systems
In batch systems, digesters are lled once with fresh FVW,
with or without addition of seed materials, and allowed to go
through all degradation steps sequentially. The hallmark of
Table 2
Performance data of different anaerobic processes applied for FVW treatment
Process Volume (l) Loading rate
(gVS/(l day))
HRT (day) VS removal (%) Methane yield
(litre/gVS)
References
Batch system 10 1.06 47 65 0.16 [35]
Batch system 5 0.9 32 58 0.26 [36]
Continuous one-stage CSTR 3 1.6 20 88 0.47 [3]
Continuous one-stage CSTR 16 3.6 23 83 0.37 [20]
Continuous tubular reactor 18 2.8 20 76 0.45 [4]
Two-stage system: solid bed hydrolyser
and UASB methaniser
100 + 25 6.8 2.5 94 0.35 [52]
Two-stage system: ASBR hydrolyser and
anaerobic lter methaniser
2.5 + 10 4.4 7 + 10 87.5 0.34 [21]
Two-stage system: CSTR
hydrolyser and anaerobic
lter methaniser
7 + 4 5.65 2 + 2.3 96 0.42 [20]
batch systems is the clear separation between a rst phase,
where acidication proceeds much faster than methanogen-
esis, and a second phase, where acids are transformed into
biogas [34].
Converti et al., tested the anaerobic batch digestion of
FVW, under both mesophilic and thermophilic conditions
[9]. The results showed that, under mesophilic and ther-
mophilic conditions, the mixture of vegetable wastes was
quickly digestible, and the rst-order kinetic constant around
4.1 10
3
l/(h g) VSS was estimated for these materials.
Anaerobic batch digestion of mixed vegetable waste was
also carried out successfully at 5% total solid concentration
[35] (Table 2). Digestion of the waste after 47 days resulted
in 0.16 m
3
biogas/kg TS added with a maximum gas produc-
tion rate on day 26. Whereas, Bouallagui et al., [36] and
Marouani et al., [37] showed that the anaerobic treatment of
992 H. Bouallagui et al. / Process Biochemistry 40 (2005) 989995
FVW at 8% TS in a batch digester was inhibited by the VFA
accumulation and irreversible decreasing pH problems.
Batch systems have, up to now, not succeeded in taking
a substantial market share. However, the specic features
of batch processes, such as simple design and process con-
trol, robustness towards coarse and heavy contaminants, and
lower investment costs make them particularly attractive for
developing countries [34]. The dependence of the biometha-
nation yield on the starting level of digestible organic sub-
stances observed in batch digestion tests suggested the op-
erating conditions for the fed-batch or continuous digestion
of the materials under consideration.
Application of sequencing batch reactor (SBR) technol-
ogy in anaerobic treatment of FVW is of interest because of
its inherent operational exibility, characterised by a high
degree of process exibility in terms of cycle time and se-
quence, no requirement for separate clariers, and retention
of a higher concentration of slow-growing anaerobic bac-
teria within the reactor [38]. Research into the ASBR pro-
cess has been carried out by several investigators [39,40].
Satisfactory high solid content waste degradation and sus-
pended solid removal (9093%) using the ASBR were re-
ported [41,42].
3.2. Continuous one-stage systems
About 90% of the full scale plants, currently in use in Eu-
rope for the anaerobic digestion of organic fraction of munic-
ipal solid wastes and biowastes, rely on continuous one-stage
systems [14]. However, a considerable amount of literature
has appeared concerning wastes treatment in two phases;
rst an acid forming phase followed by a methanogenic
phase [4345]. A likely reason for this discrepancy is that
two-and multistage systems afford more possibilities to the
researcher to control and investigate the intermediate steps
of the digestion process. Industrialists, on the other hand,
prefer one-stage systems because of their simpler designs
and lower investment costs.
Different experiments on vegetable wastes anaerobic di-
gestion were carried out using different one-stage systems
(Table 2). Mata-Alvarez et al. examined the performance of
the mesophilic one-stage completely stirred reactor (Fig. 2a)
for the treatment of the organic fraction of the wastes coming
from a large food market [3]. The maximum organic load-
ing rate (OLR) tested was below 3 kg TVS/(m
3
day). The
OLR of 6 kg TVS/(m
3
day) was found to be a limit condition
for a similar waste digestion [31]. Moreover, as mentioned
by Mata-Alvarez et al., this waste was presumably more
biodegradable, which meant a larger and faster VFA pro-
duction which stressed the validity of this OLR limit [32].
Overloading of digesters with FVW above 4 kg TVS/m
3
day
was also reported by Lane to result in a fall in pH and gas
yield and an increase in the CO
2
content of gas produced
using a continuously stirred tank reactor (CSTR) [46].
A semi-continuously mixed tubular digester was tested
(Fig. 2b) [4,47]. The best results were obtained by applying
an HRT of 20 days with an OLR of 2.8 kg TVS/(m
3
day).
The pH may fall in the hydrolysis shortly to 6.1, but it
remains most of the time at 7.2. When reducing the HRT to
10 days, the pH fell to 5 and inhibition was observed. The
most signicant factor of the tubular reactor is its ability
to separate acidogenesis and methanogenesis longitudinally
down the reactor, allowing the reactor to behave as a system
of two phases.
In one-step anaerobic digestion of solid wastes, problems
may occur if the substrate is easily degradable because in
solid waste digestion, there is no possibility for the accu-
mulation/retention of biomass within the reactor, the slower
growing methanogens are overfed at higher loading rates [6].
In a one-stage system, combining acidogens and
methanogens in one vessel, hydrogen formed by acidogenic
metabolism is assimilated by the methanogens to reduce
carbon dioxide to methane and water [48]. On increasing the
feeding rate of the substrate, acidogenic activity, including
mainly acetate, carbon dioxide, and hydrogen production,
is increased, whereas the methanogenic population cannot
increase its activity to the same extent. At a loading rate,
were the hydrogen consuming reactions become saturated,
accumulation of hydrogen partially inhibits its further for-
mation and consequently more organic electron sink will
be formed, causing imbalances and cessation of methane
production [49,50].
3.3. Continuous two-stage systems
Both groups of acidogenic and methanogenic organisms
are different with respect to their nutritional requirements,
physiology, pH optima, growth, and nutrient uptake kinet-
ics, and their ability to withstand environmental stress fac-
tors [13]. With conventional digestion processes, by com-
bining acidogens and methanogens in one reactor, uniform
conditions are imposed on both groups. However, two-phase
anaerobic digestion implies a process conguration employ-
ing separate reactors for acidication and methanogenesis
connected in series, allowing optimisation of both processes
[51].
The two-phase anaerobic digestion of a mixture of
fruit and vegetable wastes was studied in different works
(Table 2). The two-step technology applied by Rajeshwari
et al., allowed the conversion of over 94% of vegetable
market waste into biogas (Fig. 2c) [52]. The raw waste
was acidied in a solid bed reactor. The leachate obtained
after completion of acidication phase was further treated
in an UASB reactor for biogas production. A different kind
of FVW have been subjected to two-phase anaerobic di-
gestion [21]. The hydrolysisacidication step was carried
out in ASBR and methane fermentation was performed in
a xed lm reactor operated in the upow mode (Fig. 2e).
The global degradation yield remained above 87% and the
biogas production yield was about 0.29 l per g of input total
COD. Using a two-stage system involving a thermophilic
H. Bouallagui et al. / Process Biochemistry 40 (2005) 989995 993
Fig. 2. Processes used for FVW anaerobic treatment: (a) continuously stirred tank reactor (CSTR) [3,6]; (b) tubular reactor [4,47]; (c) two-phase integrated
anaerobic solid bed hydrolyser (SBH) and upow anaerobic sludge blanket (UASB) [52]; (d) two-phase integrated anaerobic continuously stirred tank
reactor and xed lm reactor (FFR) [20]; and (e) two-phase integrated anaerobic sequencing batch reactor and xed lm reactor (FFR) [21].
liquefaction CSTR reactor and a mesophilic anaerobic lter,
more than 95% volatile solids were converted to methane
at a volumetric loading rate of 5.65 g VS/l d (Fig. 2d). The
methane production yield was about 420 l/kg added VS [20].
These authors generally found that phase-separated di-
gesters may offer the best choice for high efciency, con-
cerning both depuration rates and energy recovery.
4. Post-treatment of anaerobic digestion efuent
Post-treatments are necessary if anaerobic efuents need
to be discharged into surface waters, because anaerobic di-
gestion alone is not able to produce efuents that can meet
the discharge standards applied in most industrialized coun-
tries, particularly for COD and nitrogen [53]. Up to now,
there is a denite lack of practical experience and know-how
in the treatment of those efuents [54]. In view of the in-
creasingly wide acceptance awarded to the fermentation pro-
cess, it appears necessary to include the treatment of the gen-
erated wastewater in the overall process and to grant it the
same priority as the fermentation step. The SBR technology
can successfully be used for carbon and nitrogen removals
with anoxic/aerobic processes. Garrido et al. reported that
98 and 99% removals were achieved with the conventional
SBR reactor for COD and nitrogen, respectively [55].
5. Conclusion
Anaerobic digestion represents a commercially viable
process to convert FVW to methane gas, a useful energy
source. The overall results of anaerobic digestion of FVW
suggest that the two-stage system is a promising process
to treat these wastes with high efciency in term of degra-
dation yield and biogas productivity. This efciency is
994 H. Bouallagui et al. / Process Biochemistry 40 (2005) 989995
possible by the adaptation of each ecosystem to its own
substrate. The biochemical reactions involved in anaerobic
digestion of FVW are taken subsequently under conditions
similar to those of the rumen. It is appropriate to view the
gastrointestinal tract as an ecological system and that by
applying ecological principles, a better understanding of
distribution and interaction of organisms can be achieved,
and then it could help to design and construct a suitable
bioreactor for FVW anaerobic treatment.
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