Histology Self Quiz

Bone Bone is a type of _____Connective tissue ______________________. A bone can be

an ____organ__________ as several types of tissues are present. Functions 1.

Support 2.

ineral Storage !.

"rotection #.

ove$ent %.

Bloo& cell pro&uction # 'ypes of Cells in Bone 1.

(steoprogenitor cells 2.

(steoblasts !.

(steocytes #.

(steoclasts (steogenitor cells &erive fro$ $esenc)y$al cells. ')ey are foun& in
t)e *nner$ost layer of t)e periosteu$. (steogenitor cells actually &i+erentiate into
osteoblasts. (steogenitor cells ai& in bone gro,t) an& repair (steoblasts are
i$$ature bone cells t)at synt)esize e-tracellular $atri-. ')e e-tracellular $atri-
consists of type 1 collagen. glycoproteins. an& proteoglycans. An osteocyte is a
$ature bone cell t)at rests in a lacuna ')e process of bone for$ation is calle&
osteogensis ')e e-tracellular $atri- before it is )ar&ene& is calle& osteoi&. (steoi&
is )ar&ene& ,)en )y&ro-yapetite for$s a crystalline structure on collagen. ')e
for$ation of bone occurs at _____)ig)__________ levels of
_________o-ygen___________. (steocytes are lin/e& t)roug) tunnels calle&
____canicula______ by pro&ucing ____processes_____ ,)ic) e-ten& t)roug)
_____canicula_______ interconnecting cells an& bloo& supply. An osteoclast is a
$ultinucleate cell t)at causes t)e &egra&ation an& reabsorption an& re$o&eling _
of bone

')e __subosteoclastic co$part$ent ___ is t)e p)ysical space in bone create& by t)e
osteoclast ,)en it is attac)e& to bone ')e _resorption bays __ are p)ysical spaces
in bone create& by osteoclasts once it is re$ove& fro$ bone. ')e t)ree zones of t)e
osteoclast 1.

Basal 0one1 'op of t)e osteoclast. furt)est fro$ bone 2.

Clear 0one1 ,)ere osteoclast $a/es contact ,it) t)e bone !.

2esicular 0one 1 Contains t,o types of secretory vesicles. 3)at are t)ey4 5lysoso$al
an& calciu$6 Bone 7ro,t) All bone gro,t) is appositional 8n&osteu$ is connective
tissue t)at lines $arro, cavities "eriosteu$ is connective tissue t)at lines t)e
e-ternal surface of bone. 3)at are t)e t,o layers of t)e periosteu$4 1. *nner cell
layer 5osteoprogenitor cells. osteoblasts6 an& t)e (uter 9brous layer 5collagen.
9broblasts. bloo& vessels6 3)at are t)e t,o types of bone4 1.

Cancellous bone: Spongy bone 5$ore soft tissue space t)an of bone $atri-. Soft
spaces contain bloo& vessels. nu$erous interconnecting cavities. 2.

Co$pact bone: &ense bone1 &ense areas ,it)out cavities 'rabeculae; ')e spicules
seen ,it) spongy bone 3)at are t)e t,o types of bone $arro,4 <e&5$ostly
)e$atopoetic tissue6 an& yello, 5$ostly fat6 "ri$ary bone1 t)e 9rst bone to appear
&uring e$bryonic &evelop$ent an& fracture repair. =sually te$porary. Secon&ary
bone1 Bone foun& pri$arily foun& in a&ults. ,)ic) )as $ultiple layers of calci9e&
$atri-. ')e layers are calle& la$ellae. Co$pact bone is co$pose& of osteons. t)e
cylin&rical structure ,it)in bot). For$ation of an osteon 1.

(steoprogenitor cells are intro&uce& t)roug) a ne, bloo& supply in t)e bone an&
line up aroun& a ,all in t)e bone.

2.

(steoprogenitor cells &i+erentiate into osteoblasts. !.

(steoblasts begin $a/ing osteoi&. #.

(steoi& beco$es $ineralize& trapping $ature osteocytes ,it)in t)e bone. >escribe
bloo& supply in osteons1 Bloo& supple runs t)roug) an& bet,een osteons to ensure
nutrients in constant supplt. 2ol/$anns canals are )orizontal tunnels t)at carry
nutrients an& t)e central canal &oes too. >escribe t)e &i+erent ,ays you can
prepare a )istological section of bone1 1.

>ecalci9cation1 ?ou can &ecalcify a section of bone ,it) aci&s or a c)elating agent.
')en t)e bone tissue is soft an& can be e$be&&e& in ,a- an& sectione& easily. 2.

7roun& sections1 ?ou can grin& &o,n bone until it is t)in enoug) to loo/ at. Cells
an& tissue is &estroye& but caniculi an& cell lacunae are easy to see. !.

Sections of non&ecalci9e& bon1 you can e$be& bone is )ar& resin an& section it
,it) a special /nife 5&ia$on& /nife6 2 types of Bone For$ation 1.

*ntra$e$branous Bone For$ation 2.

8n&oc)on&ral Bone For$ation