Академический Документы
Профессиональный Документы
Культура Документы
Bioresource Technology
journal homepage: www.elsevier.com/locate/biortech
h i g h l i g h t s
g r a p h i c a l a b s t r a c t
Characterization of agarophytic
a r t i c l e
i n f o
Article history:
Received 19 December 2013
Received in revised form 18 February 2014
Accepted 22 February 2014
Available online 3 March 2014
Keywords:
Agarophytes
Agar
Cellulose
Pigments
Protein
a b s t r a c t
The major seaweed components such as natural colorants (R-phycoerythrin (R-PE), R-phycocyanin
(R-PC)), minerals, proteins, lipids, cellulose and agar which are of considerable commercial value were
estimated in 15 different agarophytic seaweeds. R-PE and R-PC contents ranged from 138.33 17.67
to 1039.43 27.65 lg/g and 50.26 6.63 to 818.2 48 lg/g on fresh weight (fw) basis, respectively.
Appreciable amounts of both macro-minerals (K, Na, Ca, Mg) and micro-minerals (Fe, Zn, Se, Mn) were
estimated. The total lipid and protein contents were 0.65 0.06% to 1.53 0.07% and 4.75 0.5% to
19.31 3.5% on dry weight (dw) basis respectively while cellulose and agar contents varied from
3.7 0.13% to 12.20 0.45% and 9.17 0.62% to 25.23 0.50% dw, respectively. The overall nding of this
study enable the selection and value addition of agarophytic feedstock for biorenery.
2014 Elsevier Ltd. All rights reserved.
1. Introduction
Marine macroalgae are harvested worldwide from both cultivated farms and wild stocks, and used as feedstock for food,
phycocolloids, phytosupplements (soil additives, fertilizers),
pharmaceuticals, nutraceuticals and cosmetics (Hanisak, 1998;
McHugh, 2003). Phycocolloids are considered as one of the major
commercial seaweed extracts with a market value over US$ 1 billion. Among the phycocolloid yielding seaweeds harvested and
processed worldwide, agarophytes constitute about 72,000 dry
tonnes from which ca. 9600 tonnes of agar produced annually with
a market value over US$173 million (Bixler and Porse, 2011).
Agarophytes are typically the red algae that include many commercially exploited genera Gracilaria, Gelidium and Gelidiella. The
members belonging to the genera of Gracilaria and Gelidium are
the major agar producers in the world, while Gelidiella acerosa is
the preferred source of agar in India (McHugh, 2003). Farming of
Gracilaria is being carried out in several countries including the
Philippines, Chile, China, Korea, Indonesia, Namibia, Vietnam and
Argentina at a commercial scale (McHugh, 2003). Some of the
281
R PC 0:154A618 A730
R PE 0:1247A564 A730 0:4583A618 A730
2.3. Mineral composition analysis
For mineral composition, 100 mg grounded dried samples were
treated with 10 ml of conc. HNO3 overnight (Santoso et al., 2006).
Thereafter, 2.5 ml of conc. HClO4 and 250 ll of H2SO4 were added
to the samples followed by heating until no white smoke was emitted. 100 ml of 2% HCl was added in the digested sample and ltered with a 0.22-lm membrane lter. The samples were
analyzed using inductively coupled plasma atomic emission spectroscopy (Perkin-Elmer, Optima 2000, USA).
2.4. Total protein and lipid content determination
The organic nitrogen content were quantied with dry ne
grounded sample using the instrument, Elementar Analysensysteme GmbH vario MICRO cube, calibrated using sulfanilamide as
a reference standard. The total protein contents were estimated
by multiplying the nitrogen content by a factor of 6.25 (Loureno
et al., 2002). Lipids were extracted from 500 mg dry algal samples
following the method of Bligh and Dyer (1959) and lipid content
were determined by the gravimetric method.
2.5. Cellulose extraction and characterization
Cellulose was extracted from the seaweeds samples following
the method reported by Mihranyan et al. (2004). 10 g of dried algal
282
Table 1
Description of collection sites of different agarophytic seaweeds.
Species
Collection site
Location
Gelidium micropterum
Veraval
Old Mandapam Jetty
Veraval
Valinokkam
Veraval
Adri
Nochiyoorani
Veraval
Old Mandapam Jetty Valinokkam
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
Gelidium pusillum
Gelidiella acerosa
Gelidiopsis variabilis
Gracilaria edulis
Gracilaria salicornia
Gracilaria dura
Gracilaria corticata
G. corticata v. cylindrica
Gracilaria folifera
Gracilaria textorii
Gracilaria fergusonii
Gracilaria crassa
Gracilaria debilis
Gracilaria verrucosa
Krusadai Iceland
Jegathapattinam
Veraval
Rameswaram
Veraval
Adri
Okha
Veraval
Adri
Nochiyoorani
Veraval
Adri
Krusadai Iceland
Veraval
Krusadai Iceland
Veraval
Veraval
Adri
Rameswaram
Pamban
Okha
Okha
20 54.870 ; E 70 20.830
9 17.140 ; E 79 09.530
20 54.870 ; E 70 20.830
9 09.930 ; E 78 38.070
20 54.870 ; E 70 20.830
20 57.580 ; E 70 16.760
9 15.940 ; E 79 01.980
20 54.870 ; E 70 20.830
9 17.140 ; E 79 09.530
9 09.930 ; E 78 38.070
9 14.220 ; E 79 12.300
9 57.500 ; E 79 11.610
20 54.870 ; E 70 20.830
9 18.540 ; E 79 20.360
20 54.870 ; E 70 20.830
20 57.580 ; E 70 16.760
22 27.060 ; E 69 4.020
20 54.870 ; E 70 20.830
20 57.580 ; E 70 16.760
9 15.940 ; E 79 01.980
20 54.870 ; E 70 20.830
20 57.580 ; E 70 16.760
9 14.220 ; E 79 12.300
20 54.870 ; E 70 20.830
9 14.220 ; E 79 12.300
20 54.870 ; E 70 20.830
20 54.870 ; E 70 20.830
20 57.580 ; E 70 16.760
9 19.270 ; E 79 17.710
9 16.230 ; E 79 11.300
22 28.440 ; E 69 03.580
22 28.440 ; E 69 03.580
283
Table 2
Estimated pigments content (lg/g fw) in different agarophytic seaweeds (Mean SD,
n = 3).
Species
R-phycoerythrin (R-PE)
R-phycocyanin (R-PC)
Gelidium micropterum
Gelidium pusillum
Gelidiella acerosa
Gelidiopsis variabilis
Gracilaria edulis
Gracilaria salicornia
Gracilaria dura
Gracilaria corticata
G. corticata v. cylindrica
Gracilaria folifera
Gracilaria. textorii
Gracilaria fergusonii
Gracilaria crassa
Gracilaria debilis
Gracilaria verrucosa
1039.43 27.65
887.30 98.95
684.15 38.36
935.79 33.03
373.25 13.48
138.33 17.67
383.71 19.89
657 7.93
380 26.85
752.66 3.78
193.33 25.71
264.33 14.01
237.45 11.15
249.33 28
684 10
269 12.16
445.06 42.92
595.98 43.12
818.2 48.40
276.68 23.37
50.26 6.63
180.18 24.05
323.66 10.69
268.33 30.02
611.66 3.21
54 3.4
216.66 52.54
237.16 9.24
205.66 39.92
315 6.4
(Kumar et al., 2011). However, seaweeds could not be a conventional source of energy since their lipid content is low, but their
PUFA contents can be as high as those of terrestrial vegetables
(Darcy-Vrillon, 1993; Kumari et al., 2010). Since seaweeds lipid
contained good amounts of PUFA, could be useful for nutraceuticals. Protein contents for investigated seaweeds were ranged between 4.75 0.5% and 19.31 3.5% dw (Table 4) which were
accordance to previously reported protein contents for different
seaweeds (McDermid and Stuercke, 2003; Gressler et al., 2010;
Kumar et al., 2011; Narasimman and Murugaiyan, 2012). Most of
the Gracilarials showed protein contents ranged between 4% and
10% dw except G. corticata (15.08 1.42% dw) and G. foliifera
(15.16 3.51% dw). G. pusillum, G. micropterum, G. variabilis and
G. acerosa (19.31 3.5%, 18.45 3.70%, 17.62 1.86% and
14.93 1.89%) were showed comparatively higher protein contents. Such higher protein content recorded in these seaweeds
indicates their possible utilization for commercial protein production to full human and animal nutritional requirements.
3.4. Cellulose content
The yield of cellulose were ranged from 3.7 0.13% to
12.20 0.45% dw (Table 4). The highest yield of cellulose obtained
from G. pusillum, (12.20 0.45%) followed by G. variabilis
(11.38 0.62%), G. micropterum (10.63 0.41%) and G. acerosa
(9.77 0.23). Lowest cellulose contents were recorded from Gracilaria dura (3.7 0.13%) and G. foliifera (3.77 0.10%). The cellulose
yields (3.712%) obtained from agarophytic seaweeds were
comparable to cellulose yields (213%) previously reported by
Siddhanta et al. (2009, 2011) for different seaweeds. The algal
cellulose could be used for bioenergy production.
The FT-IR spectra of cellulose obtained from G. pusillum is presented in Supplementary Fig. 1. The characteristic bands of cellulose were found to be the similar as earlier reported FT-IR
spectra for cellulose in the literature (Siddhanta et al., 2011). The
characteristics bands of cellulose were (KBr, mmax, cm1) at 3428
(OH stretching), 2923 (CH str), 1628 (bound H2O), 1434 (CH
bending) and 1022 (COC bending) found (Supplementary Fig. 1).
3.5. Agar contents and agar physical properties
The agar yields for the investigated seaweeds were ranged from
9.17 0.62% to 25.23 0.50% dw (Table 5) which were in accordance with yields reported earlier for different seaweeds in literature (Mehta et al., 2010; Meena et al., 2008; Prasad et al., 2007).
Highest yield of agar recorded from G. pusillum (25.23 0.50%) followed by G. dura (25.15 0.78%) and G. acerosa (24.5 0.7%).
284
Table 3
Major minerals and trace elements (mg/100 g dw) determined by atomic absorption spectrophotometry in different agarophytic seaweeds (Mean SD, n = 2).
K
Na
Ca
Mg
Fe
Mn
Mo
Ni
Se
Zn
K
Na
Ca
Mg
Fe
Mn
Mo
Ni
Se
Zn
G. micropterum
G. pusillum
G. acerosa
G. variabilis
G. edulis
G. salicornia
G. dura
G. corticata
831.9 10.60
1473.6 6.22
484.4 3.67
399.8 2.26
25.8 0.01
3 0.01
1.2 0.84
0.7 0.14
16.4 0.56
7.4 0.28
1515.1 28.14
361.3 6.92
727.1 23.61
237.1 12.02
47 0.84
4.8 0.01
1.2 0.28
1 0.01
15.1 0.14
5.1 1.27
5665.5 3.53
2394.5 113.84
377.85 86.05
607.25 122.68
64.55 2.89
5.1 0.01
0.75 0.07
1 0.14
0.7 0.01
24.55 1.76
1159.5 57.27
5217 52.32
1643.5 11.45
855.3 14.00
64.1 0.98
5 0.01
1.8 0.28
2.5 0.14
9.8 0.28
5 0.01
13715 162.63
2208 8.48
672.6 27.71
349.4 14.70
58.3 0.42
11.3 0.14
1.7 0.14
0.8 0.01
9.6 1.97
6.7 0.14
15033 69.29
3732 104.65
881.5 20.22
510.5 12.86
70.8 0.28
5.1 0.14
0.9 0.70
0.8 0.01
10.1 2.40
3.8 0.28
13175 968.73
4483 270.11
253.75 6.71
441.55 8.131
33.55 2.33
9.65 0.07
0.9 0.14
0.35 0.07
1.55 0.91
5.9 0.14
7910 29.69
2680 87.64
310.65 34.011
361.5 36.76
21.65 2.05
12.55 0.21
0.75 0.07
0.9 0.14
1.45 0.07
16 0.56
G. c. v. cylindrica
G. foliifera
G. textorii
G. fergusonii
G. crassa
G. debilis
G. verrucosa
9575 1718.26
2947.5 531.03
336.05 89.30
521.7 85.70
30.4 10.04
9.7 1.41
0.65 0.21
1 0.14
2.45 0.35
10.9 1.41
9491 572.75
3114.5 183.14
243.2 1.83
334.85 16.75
15.7 2.12
4.9 0.14
0.6 0.14
0.95 0.21
0.95 0.21
7.3 1.13
12960 664.68
3150.5 405.17
404.9 24.18
501.5 45.25
31.7 0.84
14.8 8.28
0.7 0.42
0.75 0.21
2.6 0.28
13.25 1.06
11139 1132.78
3015 472.34
255.8 5.09
397.15 49.99
32.25 7.0
5.35 0.49
1.45 1.06
1.15 0.35
1.65 0.21
5.95 1.76
11170 686.94
4105 682.0
255.63 5.76
438.5 7.78
29.7 2.30
8.33 2.28
0.66 0.15
0.366 0.05
ND
6.33 0.75
11480 325.26
4410.5 84.14
506.25 181.93
429.4 140.29
26 2.54
11.2 0.70
0.75 0.07
0.75 0.07
1.7 0.28
8.1 1.13
7924 317.78
4524 213.54
305.9 5.51
498.3 41.01
8.8 2.54
7.4 0.424
1.1 0.14
0.4 0.01
2.25 0.318
4.05 0.07
Lipid
Protein
Cellulose
G.
G.
G.
G.
G.
G.
G.
G.
G.
G.
G.
G.
G.
G.
G.
1.08 0.09
1.32 0.06
1.41 0.04
1.27 0.05
0.98 0.03
1.2 0.04
1.02 0.06
1.53 0.07
1.49 0.10
1.47 0.04
0.76 0.04
0.65 0.06
1.30 0.05
1.26 0.09
1.34 0.06
18.45 3.70
19.31 3.5
14.93 1.89
17.62 1.86
9.83 2.78
4.75 0.5
5.33 0.69
15.08 1.42
9.35 0.62
15.16 3.51
9.56 1.05
8.91 2.42
5.18 0.64
7.06 0.40
7.87 3.25
10.63 0.41
12.20 0.45
9.77 0.23
11.38 0.62
4.88 0.12
4.90 0.15
3.7 0.13
5.27 0.25
6.08 0.19
3.77 0.10
4.62 0.24
4.20 0.21
7.21 0.20
NS
NS
micropterum
pusillum
acerosa
variabilis
edulis
salicornia
dura
corticata
c. v. cylindrica
foliifera
textorii
fergusonii
crassa
debilis
verrucosa
Table 5
Yield, gel strength and gelling temperature of agar extracted from different
agarophytic seaweeds (Mean SD, n = 3).
Species
Agar yield
(%)
Gelling
temperature(C)
G.
G.
G.
G.
G.
G.
G.
G.
G.
23.54 0.52
25.23 0.50
24.5 0.7
NS
17.83 0.40
15.06 0.61
25.15 0.78
23.62 0.74
18.68 0.86
720 20
750 30
423 15
>100
256 15
250 10
103 5
>100
36 0.5
44 0.5
38.5 0.5
43.5 0.5
45 0.5
33 0.5
40.5 0.5
36 0.5
18.29 0.54
11.18 0.62
9.17 0.62
21.14 0.82
NS
NS
>100
>100
>100
290 14
35.5 0.5
35 0.5
33 0.5
38.75 1
G.
G.
G.
G.
G.
G.
micropterum
pusillum
acerosa
variabilis
edulis
salicornia
dura
corticata
c. v.
cylindrica
foliifera
textorii
fergusonii
crassa
debilis
verrucosa
of bulk products from feedstock. The advantage of such downstream process is that it will enable valorization of seaweed biomass for a spectrum of bio-products of high value. As
decolorization and defatting improves the extraction and quality
of various polysaccharides, therefore integrated biorenery approach enable fractionation of these two valuable products i.e. pigment and lipid prior to polysaccharide extraction. Despite several
advantages of seaweed biorenery concept, the eld is yet to gain
the momentum due to several factors including lack of cost effective offshore farming techniques, integrated downstream process,
value addition of fractionated products, etc. The offshore farming
together with setting up small scale bioreneries in coastal areas
can some extent offset the high production cost by reducing the
transportation cost involved in bringing the biomass from its production to processing place.
4. Conclusion
Agarophytic seaweeds investigated in this study were found
rich in natural pigments, minerals, proteins and cellulose content
apart from agar. Most of these products have historically established global markets with immense economic value. Biomass
characterization carried out in this study will aid in development
of downstream process for sequential recovery of these products
in an integrated manner from feedstock. The viability and sustenance of algal biorenery is largely depends on the biomass valorization technologies employing eco-friendly downstream process.
Acknowledgements
The nancial support received from Project CSC 0116 BioEn is
gratefully acknowledged. Authors would like to acknowledge
Head, Analytical sciences, CSIR-CSMCRI for permitting to use
instrumentation facilities.
Appendix A. Supplementary data
Supplementary data associated with this article can be found, in
the online version, at http://dx.doi.org/10.1016/j.biortech.2014.
02.083.
References
Ayyagari, M.S., Pande, R., Kamtekar, S., Marx, K.A., Tripathy, S.K., Gao, H., Kumar, J.,
Akkara, J.A., Kaplan, D.L., 1995. Molecular assembly of proteins and conjugated
polymers: toward development of biosensors. Biotechnol. Bioeng. 45, 116125.
Baghel, R.S., Kumari, P., Reddy, C.R.K., Jha, Bhavanath, 2014. Growth, pigments, and
biochemical composition of marine red alga Gracilaria crassa. J. Appl. Phycol.
http://dx.doi.org/10.1007/s10811-014-0250-5.
Bixler, H.J., Porse, H., 2011. A decade of change in the seaweed hydrocolloids
industry. J. Appl. Phycol. 23, 321335.
Bligh, E.G., Dyer, W.J., 1959. A rapid method of total lipid extraction and
purication. Can. J. Biochem. Biophysiol. 37 (8), 911915.
Darcy-Vrillon, B., 1993. Nutritional aspects of the developing use of marine
macroalgae for the human food industry. Int. J. Food Sci. Nutr. 44, 2335.
Denis, C., Ledorze, C., Jaouen, P., Fleurence, J., 2009. Comparison of different
procedures for the extraction and partial purication of R-phycoerythrin from
the red macroalga Grateloupia turuturu. Botanica Marina 52, 278281.
Dumay, J., Clment, N., Moranais, M., Fleurence, J., 2013. Optimization of hydrolysis
conditions of Palmaria palmata to enhance R-phycoerythrin extraction.
Bioresour. Technol. 131, 2127.
Eswaran, K., Ghosh, P.K., Siddhanta, A.K., Patolia, J.S., Periasamy, C., Mehta, A.S.,
Mody, K.H., Ramavat, B.K., Prasad, K., Rajyaguru, M.R., Reddy, C.R.K., Pandya, J.B.,
Tewari, A., 2005. Integrated method for production of carrageenan and liquid
seaweed fertilizer from fresh seaweeds. US Patent No. 6, 893, 479.
Gressler, V., Yokoya, N.S., Fujii, M.T., Colepicolo, P., Mancini, J., Torres, R.P., Pinto, E.,
2010. Lipid, fatty acid, protein, amino acid and ash contents in four Brazilian red
algae species. Food Chem. 120, 585590.
Hanisak, M.D., 1998. Seaweed cultivation global trends. World Aquacult. 29, 1821.
Khambhaty, Y., Mody, K., Gandhi, M.R., Thampy, S., Maiti, P., Brahmbhatt, H., 2012.
Kappaphycus alvarezii as a source of bioethanol. Bioresour. Technol. 103, 180
185.
Kumar, G., Sahoo, D., 2011. Effect of seaweed liquid extract on growth and yield of
Triticum aestivum var. Pusa Gold. J. Appl. Phycol. 23, 251255.
Kumar, M., Kumari, P., Trivedi, N., Shukla, M.K., Gupta, V., Reddy, C.R.K., Jha, B., 2011.
Minerals, PUFAs and antioxidant properties of some tropical seaweeds from
Saurashtra coast of India. J. Appl. Phycol. 23, 797810.
285
Kumari, P., Bijo, A.J., Mantri, V.A., Reddy, C.R.K., Jha, B., 2013. Fatty acid proling of
tropical marine macroalgae: an analysis from chemotaxonomic and nutritional
perspectives. Phytochemistry 86, 4456.
Kumari, P., Kumar, M., Gupta, V., Reddy, C.R.K., Jha, B., 2010. Tropical marine
macroalgae as potential sources of nutritionally important PUFAs. Food Chem.
120, 749757.
Kumar, S., Gupta, R., Kumar, G., Sahoo, D., Kuhad, R.C., 2013. Bioethanol production
from Gracilaria verrucosa, a red alga, in a biorenery approach. Bioresour.
Technol. 135, 150156.
Loureno, S.O., Barbarino, E., De-Paula, J.C., Pereira, L.O.D.S., Marquez, U.M.L., 2002.
Amino acid composition, protein content and calculation of nitrogen-to-protein
conversion factors for 19 tropical seaweeds. Phycol. Res. 50 (3), 233241.
McDermid, K.J., Stuercke, B., 2003. Nutritional composition of edible Hawaiian
seaweeds. J. Appl. Phycol. 15, 513524.
McHugh, D.J., 2003. A guide to the seaweed industry. FAO Fisheries Technical Paper
No. 441. FAO, Rome, p. 105.
Meena, R., Prasad, K., Siddhanta, A.K., 2011. Preparation of superior quality products
from two Indian agarophytes. J. Appl. Phycol. 23, 183189.
Meena, R., Prasad, K., Ganesan, M., Siddhanta, A.K., 2008. Superior quality agar from
Gracilaria species (Gracilariales, Rhodophyta) collected from the Gulf of
Mannar, India. J. Appl. Phycol. 20, 397402.
Mehta, G.K., Meena, R., Prasad, K., Ganesan, M., Siddhanta, A.K., 2010. Preparation of
galactans from Gracilaria debilis and Gracilaria salicornia (Gracilariales,
Rhodophyta) of Indian waters. J. Appl. Phycol. 22, 623627.
Mihranyan, A., Llagostera, A.P., Karmhag, R., Strmme, M., Ek, R., 2004. Moisture
sorption by cellulose powders of varying crystallinity. Int. J. Pharm. 269, 433
442.
Mondal, D., Sharma, M., Maiti, P., Prasad, K., Meena, R., Siddhanta, A.K., Bhatt, P.,
Ijardar, S., Mohandas, V.P., Ghosh, A., Eswaran, K., Shah, B.G., Ghosh, P.K., 2013.
Fuel intermediates, agricultural nutrients and pure water from Kappaphycus
alvarezii seaweed. RSC Adv. 3, 1798917997.
Naidu, K.A., Sarada, R., Manoj, G., Khan, M.Y., Swamy, M.M., Viswanatha, S., Murthy,
K.N., Ravishankar, G.A., Srinivas, L., 1999. Toxicity assessment of phycocyanin A
blue colorant from blue green alga Spirulina platensis. Food Biotechnol. 13, 51
56.
Narasimman, S., Murugaiyan, K., 2012. Proximate composition of certain selected
marine macro-algae form Mandapam Coastal Region (Gulf of Mannar),
Southeast Coast of Tamil Nadu. Int. J. Pharm. Biol. Arch. 3 (4), 918921.
Niu, J., Xu, M., Wang, G., Zhang, K., Peng, G., 2013. Comprehensive extraction of agar
and R-phycoerythrin from Gracilaria lemaneiformis (Bangiales, Rhodophyta).
Indian J. Geo-Mar. Sci. 42 (1), 2128.
Pangestuti, R., Kim, S.K., 2011. Biological activities and health benet effects of
natural pigments derived from marine algae. J. Funct. Food 3, 255266.
Prasad, K., Siddhanta, A.K., Ganesan, M., Ramavat, B.K., Jha, B., Ghosh, P.K., 2007.
Agars of Gelidiella acerosa of west and southeast coasts of India. Bioresour.
Technol. 98, 19071915.
Sampath-Wiley, P., Neefus, C.D., 2007. An improved method for estimating Rphyoerythrin and R-phycocyanin contents from crude aqueous extracts of
Porphyra (Bangiales, Rhodophyta). J. Appl. Phycol. 19, 13129.
Santoso, J., Gunji, S., Yoshie-Stark, Y., Suzuki, T., 2006. Mineral contents of
Indonesian seaweeds and mineral solubility affected by basic cooking. Food
Sci. Technol. Res. 12, 5966.
Shah, M.T., Zodape, S.T., Chaudhary, D.R., Eswaran, K., Chikara, J., 2013. Seaweed sap
as an alternative liquid fertilizer for yield and quality improvement of wheat. J.
Plant Nutr. 36, 192200.
Siddhanta, A.K., Prasad, K., Meena, R., Prasad, G., Mehta, G.K., Chhatbar, M.U., Oza,
M.D., Kumar, S., Sanandiya, N.D., 2009. Proling of cellulose content in Indian
seaweed species. Bioresour. Technol. 100, 66696673.
Siddhanta, A.K., Chhatbar, M.U., Mehta, G.K., Sanandiya, N.D., Kumar, S., Oza, M.D.,
Prasad, K., Meena, R., 2011. The cellulose contents of Indian seaweeds. J. Appl.
Phycol. 23, 919923.
US Department of Agriculture, Agricultural research service. 2001. USDA food
search for windows, version 1.0, Database version SR18.