Jatropha curcas L.

Cultivation Experience in
Karachi Pakistan
Joint Study Preliminary Report of

Pakistan Agricultural Research Council

And
Pakistan State Oi

by

Syed Asim Rehan Kazmi*

Mr. Abdul Hameed Solangi
and Syed Nawaid Anjum Zaidi**
_____________________________________________________________________________________
*= Director, Coastal Agricultural Research Station, PARC, Jinnah Avenue, Malir Halt, Karachi
**=Deputy General Manager, Alternate Energy, PSO House, Clifton Karachi

Annexure A

Rationale:
Diminishing fossil fuel resources, unstable/increasing prices of oil, environmental issues, global warming / climatic
shifts, and allied problems have lead the world to focus on alternate, environment friendly and renewable energy sources. Among
many candidates expedited, production of bio-diesel from Jatropha curcas L. has gained significant importance. More, than 40
countries of the world are evaluating the potential of using this pure plant oil for manufacturing diesel. Pakistan as a developing
country also faces difficulty in meeting its energy requirement mainly from the imported oil, hence, has to allocate/commit a
major component of its valuable foreign exchange for its import every year. It has sufficient potential to cultivate Jatropha curcas
L. on large scale in the country preferably without comprising for land and water under food, feed and cash crops.
Pakistan is arid to semi-arid country, located between the longitude 61 east to 76 east and between latitude
23 north to 37 north. Total area of Pakistan is 79.61 million hectares. Agriculture is the main stay of Pakistan's economy,
contributing 35 percent to the gross domestic product and providing 60 percent of the labour force. Moreover, nearly 60 percent
of the total export of the country originates from agriculture. Total annual cropped is about 19.72 million hectares. Out of which,
15.3 million hectares are irrigated areas, about 75 % (11.4 mha.) is irrigated through canals, l9 % (2.9 mha.) through tube wells 2
% (0.3 mha.) through wells and remaining 4 %(0.4 mha.) through tanks and other sources. The climate of the country is
favourable for two crop's season under irrigated during the year. The mean annual rainfall varies from less than 100 mm in Sindh
to more than 1000 mm in the foot-hills and northern mountains with an average of about 400 mm. About 60% of this rain comes
during the monsoon season (July through September). Much of the summer rains are not available for crop production due to
rapid run-off because of torrential showers. At other occasions, rain may be so light that the precipitation evaporates before the
water can penetrate into the root zone. However, the contribution of rain to crops in the irrigated areas of Indus Basin is estimated
at about 1650 thousand hectares meter.
With the above said considerations Coastal Agriculture Research Station (CARS) PARC, Saleh Muhammad Goth
Karachi initiated evaluation of Jatropha curcas L. (Bio-diesel plant) in February 2007 when a few seeds of this species were sent
by Dr. Zahoor Ahmed for checking their performance at the station. In March 2008 interaction with Pakistan State Oil (PSO) was
developed who realizing their national responsibilities in maintaining oil supplies in the country felt committed to play an active
role in expediting potential of green alternate energy sources by focusing their activities in introducing and popularizing Jatropha
curcas L. in the country.
Before sharing the outcome of this joint venture so far it would be appropriate to reproduce the biography of Jatropha
curcas L. as available in the literature and world wide webs on internet.

Jatropha Curcas L.:

The plant has originated in Central America and spread to other tropical and subtropical countries. It is mainly grown in
Asia and in Africa. Used mainly as a living fence to protect gardens and fields from animals. It is resistant to a high degree of
aridity and as such does not compete with food crops. The seeds contain 30-40% oil that can be easily expelled and processed to
produce a high-quality biodiesel fuel, usable in a standard diesel engine. Jatropha curcas L. (JCL) is a tall bush or small tree (up
to 5 m hight) and belongs to the euphorbia family. The genus Jatropha contains approximately 170 known species. The genus
name Jatropha derives from the Greek jatrs (doctor), troph (food), which implies medicinal uses. The plant is planted as a
hedge (living fence) by farmers all over the world around homesteads, gardens and fields, because it is not browsed by animals
Taxanomic Classification
Kingdom: Plantae Division: Magnoliophyta Class: Magnoliopsida
Order: Malpighiales Family: Euphorbiaceae Subfamily: Crotonoideae
Tribe: Jatropheae Genus: Jatropha Species: Curcas
Jatropha is primarily a tropical genus of approximately 175 succulent plants, shrubs and trees (some are deciduous, like
Jatropha curcas L.), from the family Euphorbiaceae. Jatropha is indigenous to Central America, but has been raised in other
tropical and subtropical areas in rainfall regime 200-1500 mm, such as India, Africa, and North America. Jatropha was initially
used as a valuable hedge plant and was exported to Africa and Asia by Portuguese traders. The flowers and stem of Jatropha

23

Annexure A

curcas have well-known medicinal properties, and the leaves are used for dressing wounds. The oil has been used as an organic
insecticide as well as an effective treatment for snake bites and other ailments.
The mature Jatropha trees bear male and female inflorescence, and can grow to a height of three to four meters. Each
inflorescence bears up to 10 to 15 large fruits and Jatropha generally blooms twice a year under normal conditions. Jatropha is
desirable as a source for biofuels given that its seeds produce up to 40% oil, and the species in general is highly resistant to
drought and pests. Processed oil from crushed mature seeds can be used in existing standard diesel engines, while the residue can
also be processed into biomass to power electricity plants. The seed cake, a by-product of oil production can be used as a natural
organic fertilizer rich in organic matter. Research is underway to remove the toxic element prevalent in the seed cake to render it
useful as an animal feed.
Botanical features:
Jatropha curcas L., or physic nut, has thick glabrous branchlets. The branches contain translucent, whitish latex, which causes
brown stains very difficult to remove. The tree has a straight trunk and gray or reddish bark, masked by large white patches.

Leaves: large 6-15 cm, green to pale-green leaves with 3-7

shallow lobes are arranged alternately to sub-opposite, and a spiral
phyllotaxis.
Flowers: Inflorescences are formed terminally on branches. The
plant is monoecious and flowers are unisexual. More female flowers give
more number of seeds. Pollination is by insects. The petiole length ranges
between 6-23 mm. The inflorescence is formed in the leaf axil. The flowers
are formed terminally, individually, with female flowers, usually slightly
larger. Flowering occurs in the hot seasons. In conditions where continuous
growth occurs, an unbalance of pistillate or staminate flower production
results in a higher number of female flowers. More number of female
flowers is grown by the plant if bee keeping is done along with. More
female flowers give more number of seeds.
Fruits : After pollination, a tri-locular, ellipsoidal, fruit is
formed. Normally, fruits are produced in winter, or it may produce several
crops during the year if soil moisture is good and temperatures are
sufficiently high. The exocarp remains fleshy until the seeds are mature.
Fruits are produced in winter when the shrub is leafless, or it may produce
several crops during the year if soil moisture is good and temperatures are
sufficiently high. Each inflorescence yields a bunch of approximately 10 or more ovoid fruits. A three, bi-valved cocci is formed
after the seeds mature and the fleshy exocarp dries.
Seeds: The seeds become mature when the capsule changes from green to yellow, after two to four months from
fertilization. The blackish, thin shelled seeds are oblong and resemble small castor seeds. The seeds are black and in the average
18 mm long (11 - 30) and 10 mm wide (7 - 11). The 1000 seed weight is about 727 grams there are 1375 seeds per kg in the
average.
Analysis of the Jatropha curcas seed shows the following chemical composition:
Moisture
Protein
Fat
Carbohydrates
Fiber
Ash

6.20 %
18.00 %
38.00 %
17.00 %
15.50 %
5.30 %

24

Annexure A

The oil content is 25 30% in the seeds and 50 60% in the kernel. The oil contains 21% saturated fatty acids and
79% unsaturated fatty acids. There are some chemical elements in the seed, Cursin, which are poisonous and render the oil not
appropriate for human consumption.
Roots: Normally, five roots are formed from the seeds, one tap root and 4 lateral roots. Plants from cuttings develop
only lateral roots.
Life span: The Jatropha trees take 4 to 5 years to mature fully. At that time, if Jatropha Plantation is rain fed, these
plants can yield 0.35 to 0.375 gallon of oil per tree or 375 gallons per hectare or 150 gallons per acre. If it is irrigated (3 to 5 liters
per plant every 15 days) it can be double this amount. The life-span of the Jatropha curcas L. plant is more than 50 years.
Ecology: Jatropha curcas L. is not a weed. It is not self propagating. It has to be planted. It grows well on marginal land
with more than 200 mm of rainfall per year, and it withstands long drought periods. With less than 200-300 mm it cannot grow
except in special conditions like dormancy is induced by fluctuations in rainfall and temperature/light. But not all trees respond
simultaneously. Jatropha curcas like Castor grows almost anywhere even on gravelly, sandy and saline soils. It can thrive on
the poorest stony soil. It can grow even in the crevices of rocks. The leaves shed during the winter months form mulch around the
base of the plant. The organic matter from shed leaves enhance earth-worm activity in the soil around the root-zone of the plants,
which improves the fertility of the soil. Its water requirement is extremely low (1 liter per plant per day) and it can stand long
periods of drought by shedding most of its leaves to reduce transpiration loss. Jatropha curcas is also suitable for preventing soil
erosion and shifting of sand dunes. Jatropha is a highly adaptable species and can be planted as a pure block, a boundary fence or
an intercrop in existing plantation of coconut, mango, citrus, cashew, etc. The plant may start seeding from the first year itself.
However, the yield peaks up from the third year onwards and stabilizes by the sixth year. It has an average yield of 2 kg per plant
in well irrigated conditions. The seeds of Jatropha curcas yield oil after processing is transformed into transport fuel also known
as biodiesel.
Our /Local Experience of growing Jatropha and Jatropha plantation for bio-fuels:
The Seeds:
As per literature the seed source of Jatropha has a substantial influence on the production of seed and oil. Therefore,
seeds of Jatropha from received from various sources of USA, Thailand, Indian and China origin were evaluated for production
performance, growth and flowering features, pests and diseases, salt and drought tolerance and yields under our conditions on
limited scale. The table-1 reflects the performance of respective seeds at CARS Farm, Saleh Muhammad Goth Karachi and
indicates that the seed purity and age plays an important role in seed vigor and percent germination. Post germination sapling
health, sustenance and growth also depends on the quality of reserve food available in the seed.
Table-1: Seed germination as recorded at CARS Farm, PARC, Karachi.
Source of seed/
No. of
Date of sowing
S. No.
receiving date
seeds
Pots
field

Date

IABGR/NARC 14/2/07

20

1/3/07

14/3/07

13

Germination
Pots field
%
30
20

India (PSO)

259

17/5/08

19

PSO/Thailand
1/4/08

106

17/5/08

5-9

33

31

PSO/Thailand
2/4/08

32

17/5/08

5-7

21

66

K.U/USA

25

25/5/08

/4/08

1/3/07

25

Germination
Days No.

Annexure A

Direct seeding of Indian (60), Thailand old seeds (20) and fresh seeds (20) was
carried out in soil (silt loam) without any manure and irrigation with subsoil
slightly saline (1300 ppm) water resulted in germination of 2 seeds of Indian
origin, 9 of Thai (old seeds) and 15 of fresh.
Likewise, seeds sown by PSO people at in polyethylene bags have
shown poor germination performance in seeds from Indian and Chinese origin
but the Thai seeds demonstrated high vigor and 86-99 % germination was
recorded from seeds sown in June 2008.

Land suitability:
Current documentation regarding Jatropha cultivation indicates the most soil types, such as red and black, are suitable
for raising Jatropha plantations. The Jatropha plant, however, is best suited to sandy, well-drained soil and can survive in very
poor soil and in saline conditions. Therefore, isolated, small scale plantation was maintained at CARS farm and large scale,
cultivation was carried out at PMY (Pipri Marshalling yard terminal of PSO).
Some salient characteristics of soil at CARS Farm PARC and PMY Pakistan State Oil terminal at Pipri, Karachi are
produced in tables 2 and 3.
Table-2: Physico-chemical analyses of soil of experimental area at CARS 2007.
Soil Characteristics

EC (dS/m)
PH
O.M (%)
N (%)
P (ppm)
K (ppm)
Texture

Depth
(cm)
0-15

15-30

4.73
7.58
0.619
0.030
4.0
100
Sandy clay loam

1.92
7.7
0.222
0.011
1.9
110
Sandy clay loam

26

Annexure A

Table-3: Soil Analysis Report of PMY (Site) carried out by soil testing laboratory Thatta
Date of Sample rec.:
Date of Analysis:
Sample Received by:
Thatta
Area:
Karachi

Lab.
No.

Field No.

2161
2162
2163
2164
2165
2166
2167
2168
2169

S-1 (0-6)
S-1 (6-12)
S-1 (12-18)
S-2 (0-6)
S-2 (6-12)
S-2 (12-18)
S-3 (0-6)
S-3(6-12)
S-3(12-18)

Texture

pH

Electrical
Conductivity
(dS/m)

Available
Phosphorus
(P) (ppm)

Available
Potassium
(K) (ppm)

Loam
Loam
Loam
Loam
Loam
Loam
Loam
Loam
Loam

8.5
8.4
8.7
8.4
8.4
7.3
7.3
7.5
7.4

1.0
1.0
1.0
1.2
1.2
1.0
1.4
1.3
1.3

4.0
1.6
1.6
1.6
1.6
1.6
9.2
2.4
5.6

140
160
140
300
140
100
460
180
260

Key:
pH:
Neutral 6.5-7.5,
Electrical Conductivity (dS/m):
Normal < 4.0
Available Phosphorus (ppm):
Poor <7.0
Available Potassium (ppm):
Poor <125
Sodium Adsorption Ratio (SAR): Normal < 10.o

Alkaline > 8.5

Saline > 4.0
Medium 7.0-13
Medium 125-250
Sodic > 10.0

Soluble @
Exch.
Sodium (Na)
(meq/L)
1.7
1.7
6.5
7.8
7.8
8.3
1.7
5.7
6.1

Soluble
Ca+Mg
(meq/L)
19
15
10
10
10
10
10
10
11

Satisfactory >13
Satisfactory >250

The soil samples from PSO site shown above (table-3) were obtained from the area shown in figure-1.

27

22 September 2008
26 September 2008
MDO

Sodium
Adsorption
Ratio
(SAR)
0.6
0.6
2.9
3.5
3.5
3.7
0.8
2.5
2.6

Annexure A

Aerial Mapping of Plantation Plan

B

Exist ing Plantat ion Area

Proposed Plantation Area
Water Storage ponds
Proposed Office/Sheds
Internal Tracks
Boundary Wall
Terminal Area

N ursery

Expell ing / Est r / W arehouse

Paki stan State Oi l

Figure-1: Plantation plan of PSO at PMY terminal, Pipri, Bin Qasim Town, Karachi
Land Leveling and Digging of pits:
The 70 acres area at Pipri Marshaling yard (PMY) was barren or semi barren and kept unattended for future expansion
was selected for cultivation of Jatropha. Actual aerial image of the site from Google Earth presented at figure-2 reflects
diversification of land and hence leveling and removal of wild Prosipis juliflora (Devi) plants were carried out using a bulldozer
and tractor to remove the debris.
Jatropha curcas is a deciduous shrub/tree and
hence has to be planted in pits. Digging of pits
45x45x45 cm as recommended in literature was carried
out with 2x2 meters plant to plant and row to row
distance. This strategy maintained a ratio of 1200 plants
per acre.

Figure-2: Aerial view of PMY site,

PSO, Pipri Karachi.

28

Annexure A

Refilling of pits:
The pits were filled with sweet soil, farmyard manure and de-oiled press cake of Jatropha seeds @ 2:1:1 respectively.
Although filling with soil mixed with farmyard manure (about 1 kilogram per pit), 120g (grams) SSP (single super phosphate),
and 100g neem oil cake at the time of transplanting is normally recommended. Neem oil cake is said to be essential, as it
immediately provides protection from several pests in addition to fulfilling the nitrogen demand.
Such refilling should be to the extent of half the depth of the pits (2030 cm). The seedlings are transplanted in the
centre of the pit.
Application of fertilizer:
After the establishment of the plant, 20g urea and 16g MoP (muriate of potash) should be applied. The urea
should be applied in two splits at 10g per plant.
Application of Pesticides:
The Jatropha plants can be protected from termite and fungi by using 50g methyl parathion dust per pit and
0.05% Bavistin, respectively. If the termite attack is severe, a spoonful of Chloropyrophos can be added to
the mixture.
In case pits have been dug in the past (less than 23 months), they need to be re-opened by excavating loose soil and
refilling them with the desired planting mixture.
Nursery:
A separate nursery area has been built by PSO at PMY Karachi measuring 60x180 to accommodate 20,000 seedlings
at a time. This nursery can be seen in photos below;

Planting medium:
There are different types of media in which a seed will grow. The media can be chosen depending on type of plant that
is to be grown. For general purpose, the best medium is a mixture of 70% red soil, 20% dry cow dung manure and 10% river
sand. Peat moss or vermiculite can be mixed with the potting medium to increase the porosity of the soil. It is good to increase
the porosity of soil because the emerging seedlings are delicate and should face least resistance while coming to the surface.
Additional Medium: Peat moss or vermiculite can be mixed with the potting medium to increase the
porosity of the soil. It is good to increase the porosity of soil because the emerging seedlings are delicate and
should face least resistance while coming to the surface.
Containers: Any container with good drainage can be used to grow plants. All that is required is to see to it
that there are holes in the bottom of the container so that extra water is drained out. We have used small
polyethylene bags with perforation at the base to raise the seedlings in nursery.
Filling the containers: Fill the pots or polyethylene bags with the potting mixture and water the pot. Allow
the extra water to drain out before sowing the seeds.

29

Annexure A

Sowing of seeds: Place the seeds on the surface and cover it lightly with the soil. Depth at which the seeds
are sowed plays very important role in germination. Seeds contain only enough stored food for a limited
period of growth. If the seedling is to survive it must reach the surface quickly and begin producing its own
food for further growth. If the seeds are too deeply buried beneath the soil surface, the seedling will exhaust
its food reserve before reaching the surface causing its death. And if the seeds are left exposed without
covering it with soil even then it will die due to dehydration. Two to six seeds can be planted in a container
depending on the size of the container and the seeds.
Temperature: Place the container in shade after sowing the seeds. If possible tie a transparent plastic over
the containers mouth to reduce loss of water. If placed in direct sunlight then the temperature of the soil may
rise above the temperature required by the seed to germinate.
Watering: Do not water the container from the top. Place the container in which seeds are sown in a tray
containing water. The water will move upwards slowly and after 2-3 hours remove the container from the
tray. This way you can water the container without disturbing the seed.
Post germination care: Once you see the seedling emerging from the soil remove the plastic cover but do
not put it in direct sunlight. Keep the container with the seedlings in shade but make sure there is light as it
needs light to produce food. Let the plant grow. Make sure that you water the plant in the morning or in the
evening because if you water it during hot afternoon the plant might be adversely affected. Once your plant is
a month old then you can keep it in direct sunlight. Every month loosen the soil around the plant without
disturbing the roots as this will help oxygen reach the roots.
Post germination care: Once you see the seedling emerging from the soil remove the plastic cover but do
not put it in direct sunlight. Keep the container with the seedlings in shade but make sure there is light as it
needs light to produce food. Let the plant grow. Make sure that you water the plant in the morning or in the
evening because if you water it during hot afternoon the plant might be adversely affected. Once your plant is
a month old then you can keep it in direct sunlight. Every month loosen the soil around the plant without
disturbing the roots as this will help oxygen reach the roots.
Lifting of plants from nursery: Before removal from the nursery, plants should be thoroughly watered to
moisten the soil and to avoid desiccation during transportation until planting. There are no special problems
in removing plants grown in polybags, if the roots have not grown out and penetrated into the soil. However,
care should be taken to prevent them from desiccation, and to ensure that the ball of earth does not break.
Bare-root seedlings should never be kept exposed to wind and sunlight for a long time. After lifting them
from the nursery, and before planting them in the field, bare-root plants should be wrapped in a wet gunny
bag and kept under shade. During transit, water should be liberally sprinkled over them. They should be
transported to the destination and planted as quickly as possible, within 24 hours of uprooting.
Santing of saplings:
Planting should be ideally done at the start of the
monsoon, immediately after the first rains, when the soil has
adequate moisture. In case an assured source of irrigation is
available, planting can be done at any time during the planting
season. At the time of planting, the plants should be between 45
and 60 cm in height, sturdy, and free of diseases or pests, with
healthy green leaves. The roots of polyethylene bag plants should
not be coiled. The polythene bag should be torn carefully without
breaking the ball of earth and disposed of. The plant should be kept
straight at the top of the half-filled pit. Subsequently, the rest of the
pit

should be filled with the farm-yard manurelocal soil mixture,

and compacted around the plant with the feet. In case naked-root
seedlings are used, the plant should be kept straight in the halffilled pit, and the filled soil should be pressed hard and made

30

Annexure A

compact to keep the plant straight. Special care should be taken to prevent damage to roots.
Keeping in view the above mentioned direction 7-8 week old plants were generally transplanted in the field at PMY
and this plantation now holds over 20,000 Jatropha plants spread on 16+ acres. Plantation on remaining approximately 54 acres is
in progress.
Irrigation:
Jatropha does not require lot of water and is considered as drought tolerant. However, for commercial plantation
irrigation is beneficial in the first two months after planting, especially if the monsoon is delayed. Once the plants are established
and acclimatized, irrigation should be done on an as-and-when-required basis depending on the situation. Irrigation is required
primarily if it does not rain within a week of planting. Subsequently, it is required once during peak winter and then at the time of
fruiting. In fact irrigation at CARS and PMY Karachi were carried out keeping on the basis of 250 mm/annum water demand
that amounts to 2.5 liters of water per plant per day. Various irrigation techniques were also tested e.g. drip irrigation, using
empty plastic soft drink bottle of 1.5 liter volume etc. But all the irrigation exercises carried out were on the per plant basis
avoiding flood irrigation. Source of water has been subsoil, low quality (800-1300 ppm) saline water.
Diseases and pests
Reportedly, collar rot disease is common in Jatropha. This can be
overcome by using 1% Bordeaux solution. Beetles/hoppers/leaf miner
larvae are the common pests afflicting the plant. Endosulfan (3 ml/litre)
can be used to control these pests.
However, no pest was recorded on Jatropha at CARS compared
to PMY plantation where attack of white fly, leaf minor and roller and
mealy bugs was observed. Simple water and water + detergent spray for
controlling white fly, water + 3% diesel + detergent mix for remaining
pests were tried with a success rate of 50--70 % . However, insecticide
spray using Cypermethrin was also used on earlier nursery plantation at
PSOs Kemari terminal and once a PMY. Mealy bug was also observed
on two plants at Malir Office but no treatment has been given till to-date.

Weeding and hoeing

Weeding and hoeing operations should be done at the end of the monsoon to facilitate moisture conservation and to prevent
competition from weeds. About 23 hoeing and weeding operations are recommended and adopted at PMY plantation.
Fertilizer
Better yields are obtained on poor-quality soils if fertilizers containing small amounts of calcium, magnesium, and
sulphur are used. Mycorrhizal associations are known to aid plant growth. Neem waste and farm-yard manure are also known to
enhance plant growth. But use of Jatropha cake along with farmyard manure proved sufficient to maintain the health of plants at
PMY. At CARS since April 2007 we have applied FMY three times only and no fertilizer has been provided to plants growing at
CARS Office, Malir Halt after direct seeding of Jatropha.
Pruning
To restrict the height of the plant and to
facilitate higher flowering, pruning is advised during
dormant months (DecemberJanuary), after the fruits
have been harvested. During the first year of growth,
plants should be pruned at a height of 45 cm with a very
sharp knife or secateurs. In the subsequent years, all new
branches should be cut (DecemberJanuary) to limit them
to one-third length. Diseased, dead, excessive, weak, and
lateral branches should also be removed. This facilitates
an umbrella-shape growth resulting in higher flowering
and seed production. Further, it will also make seed
harvesting simple and convenient.

31

Annexure A

In accordance with above recommendation pruning has been done twice at PMY and non at CARS Farm or Malir Halt.
As can be seen from the photo,
Tackling plant mortality
There could be some mortality in plantations due to
out planting shock, drought, disease, fire, floods, and so on.
Such stock should be replaced in the months of February
March (in case of assured irrigation) or JulyAugust, by
following the procedures mentioned above. In case of largescale mortality (>50%), it is essential to ascertain the cause of
mortality by seeking expert opinion.
Gap filling at PMY was required only once since
the initial plantation where few plants could not survive due
to hard rocky pan down below the pit. However, increasing
the depth of pit and refilling with potting mix described
earlier resolved the issue.

General Growth: Salient vegetative and growth parameters recorded from 5 plants growing at CARS since April 2007 are
summarized in table-4.
Table-4: Growth parameters recorded at CARS/PARC Farm, Saleh Muhammed Goth, Karachi
Vegetative/ generative parameters
Jatropha curcas L. plant
S . No.
recorded
1
2
3
4
5
1
2
3
4
5
6
7

Plant height (cm)

Stem diameter (cm)
Number of branches
Length of branches (cm)
Capsule weight (grams)
Number of seeds/capsule
1000 fresh seed weight (g)

275
25
8
186
5
3
1000

264
27
5
125
4.3
2-3
850

32

335
45
52
340
5.1
3
925

275
27
20
180
4
3
900

Mean
257
20
12
180
4.4
3
875

281.5
28.8
19.4
202.2
4.56
3
910

Annexure A

The seed lost moisture with time and after 25 days average seed weight dropped to 0.650 grams compared to 0.910 g on the 2nd
day. Some of snaps showing flowers, unripe fruit, ripe and decorticated fruit along with seeds and Jatropha seeds can be seen in
the plate placed below.

Inference:
The Jatropha cultivation experience since 2007 till now has increased the confidence level towards adoption of this
exotic species in the country. Parallel experiences of some local farmers growing Jatropha at their farms/sites have also been
encouraging so far. However, being exotic species regulations should be framed simultaneously to permit import of seeds of good
quality, known origin and proven tract record if commercialized Jatropha plantation is to be addressed for bio-diesel BD-5 or
BD-10 blends in the country for reaping the benefits of Carbon sequestering /Carbon credit. Opportunities for new investments,
jobs, environment improvement and reclamation of degraded soils do exist but could not be expedited till proper legal frame
work is drafted, approved and implemented. The current agriculture practices, resources, agribusiness and exports could not be
endangered if not jeopardized by ignoring the quarantine and pest risk analysis and other relevant studies. But till such time
contained cultivation on government/selective lands is required for carrying out various experiments particularly from yield to
harvest
and
processing
levels.

33

Annexure B

2009
Cultivation Performance of Bio-diesel Plant Jatropha curcas L. in Pakistan

Syed Asim Rehan Kazmi and Syed Nawaid

Anjum Zaidi

Coastal Agricultural Research Station,

Pakistan Agricultural Research Council,
and Pakistan State Oil, Karachi

34

Annexure B

Cultivation Performance of Bio-diesel Plant Jatropha curcas L. in Pakistan

Syed Asim Rehan Kazmi*and Nawaid Anjum Zaidi**
Coastal Agricultural Research Station, Pakistan Agricultural Research Council, Karachi
and Pakistan State Oil, Karachi

Abstract:
Intensifying global concerns on energy security, high energy prices, and climate changes revived
attention towards Biofuels. National commitment for utilization of B-5 Biodiesel by 2015 to
fulfill local needs and simultaneously address the environment degradation issues lead to
examine performance of exotic non edible oil yielding plant species Jatropha curcas L. The
comparative oil studies enhance the interest in this underutilized plant species. It has the ability
to tolerate drought and saline conditions, adoptability on marginal lands, and requires
comparatively low water and other inputs. Experimental field with over 22 acres plantation
maintained at confined Pipri Marshalling Yard, PSO terminal Karachi showed promising
commercialization potential. The oil yield of 28-35.36% obtained from the first year crop has
FFA value of 0.54 (% as OA). However, the plant proved susceptible to mealy bug (apparently
papaya mealy bug) attack that has been controlled using a decoction of Neem and Gliricida
leaves.
Introduction: Mats Karlsson (2007) in the preface of UN-Energy paper on Sustainable
Bioenergy: A framework of Decision Makers says Nothing human or ecological is
straightforward. Situation regarding acceptance and adoption of existing or new biofuel feed
stocks in Pakistan is no different. Many plant species possess oil. BGIR/BSI Database, 2004
enlist 287 oil yielding plants of which 36 species yield non-edible oils that can be converted to
obtain diesel comparable to petro-diesel and a blend of two offers a fuel that has the potential to
provide energy, reduce environment degradation, and minimize burden on economy. U.S.
National Renewable Energy Labs brochure, published in 2006, enlist a number of biomass
sources that can yield biofuels. From these feed stocks edible oil sources can not be diverted to
any other use as Pakistan has to import it to meet the nutrient requirements of the population.
Among the non-edible oil plant species Castor, Neem, Pongamia and Jatropha gossypifolia are
locally found but the suitability of Jatropha curcas L. has recently been explored as a feedstock
for biodiesel production, world over. Jatropha is a genus of more than 170 species including
shrubs and trees, originating in the Caribbean (Central America) and now found through out the
tropics. The seeds of Jatropha curcas produce oil that is increasingly used for biodiesel
production, particularly in the Philippines and India. The plant is hardy, grows well on marginal
lands and can also be used to restore degraded lands. These characteristics suggest that Jatropha
curcas production, if carefully managed, may be expanded without directly competing with
natural forests or high value agricultural lands used for food production.
Pakistan geographically is among the countries where J. curcas L. plantation can sustain
and flourish on coastal area lest cultivation in agricultural area is to be avoided. Out of 79.61
million hectares only 22 million hectares or less are cropped twice a year, due to shortage of
irrigation and sweet sub-soil water. The mean annual rainfall varies from less than 100 mm in
35

Annexure B

Sindh to over 1000 mm in the foot-hills and northern mountains with an average of about 400
mm. About 60% of this rain comes during the monsoon season (July through September).
Jatropha curcas L. being less water demanding and drought tolerant may serve to mitigate sandy
desert and other marginal areas.
In addition it would facilitate to reduce deforestation and
desertification which are considered as most important and cost-efficient methods of mitigating
climate change. Tokar et. al (2007) also suggests the need for policies and
incentives to reduce deforestation during the Conference of the Parties of the Framework
Convention on Climate Change.
Thrust of utilizing the marginal/barren lands, minimizing fuel import bills and creating
opportunity of new economic activities in the desert or coastal region lead us to investigate the
performance of exotic J. curcas L. in confined vicinity at Karachi. This experience is shared
here.
Materials and Methods: Jatropha curcas L. seeds from China, India, Thailand and USA origin
were initially germinated in clay pots or directly sown in the field. Later, for mass cultivation
seeds of Thai origin were germinated in nursery area using polyethylene bags containing
planting medium (70% red soil+20%cowdung+10% river soil). The nursery plants were
transplanted in the pits of 45x45x45 cm size each to accommodate 1200 plants per acre. The pits
were filled with sweet soil, farmyard manure and de-oiled press cake of Jatropha seeds @ 2:1:1,
respectively. Plants were immediately irrigated at the rate of 2.5 liters of water per plant per day
and the trend was generally maintained except when it rained. The growth and development
parameters were recorded and plantation received usual agronomic treatments. Pest and disease
appearance and attacks were also monitored and appropriately treated when required using
simple water and water + detergent spray for controlling white fly, water + 3% diesel + detergent
mix for other pests. A decoction of fresh leaves of Azadirachta indica (Neem) and Gliricida
sepium (250g each) in one liter water was boiled till the water quantity was reduced to half was
also used after filtering through muslin cloth and cooled to room temperature before spray on the
mealy bug affected plants. This treatment was applied thrice on weekly basis. Seeds from the
harvested fruits were examined and analyzed for oil content using AOCS method by P.C.S.I.R.
Laboratories, Karachi and PSO labs carried out trans-esterification process to make biodiesel 100
and its blends. The bio-diesel and its blends were tested by using ASTM standard procedures as
mentioned in Engine Makers Association document on Test specifications for biodiesel fuel
2006 www.enginemanufacturers.org.
Results and discussion: Best germination of 66% at CARS farm was recorded in the seed
samples from Thailand provided by PSO (table-1). However, seed germination in nursery of
PSO from another lot of Thai origin was 86-99%. This indicates that the seed purity, health and
age play an important role in seed vigor and percent germination. GINWAL et al (2005) studied
seed source variation in Jatropha curcas collected from ten locations in Central India and
reported that considerable genetic variability exists in J. curcas with respect to seed morphology,
seed germination and seedling growth characteristics and concluded that in India, the
Chhindwara, source is superior among all seed sources tested. Daghar et al (2004) has shown
direct dependence of seed germination with seed weight.
Post germination sapling health, sustenance and growth also depends on the quality of
reserve food available in the seed as is evident from variation in various parameters shown in
table-2.

36

Annexure B

Large scale plantation was sown at Pipri

Marshalling Yard (PMY) of PSO that has soil
physico-chemical as shown in table-3. However, the
pits used for planting were filled with sweet soil,
farmyard manure and Jatropha seed cake @2:1:1 to
permit proper establishment of 6-8 weeks old nursery
grown saplings. The over all performance of the
seedling is evident from picture-1.
Picture-1: Jatropha curcas L. plantation at PMY.
J. curcas is a shrub or tree with smooth grey bark,
spreading branches and stubby twigs, which exude a
translucent or yellowish sap (latex) when broken. Its
height vary from 5 to 8 m, leaves are deciduous, 3- to
5-lobed in outline, 6 40 cm long and 6 35 cm
broad (Picture-2).
Flowers are yellow and bell-shaped. The plant is
monoecious, so flowers are unisexual (picture-3).
Hermaphrodite flowers may also occur as reported
by Dehgan and Webster (1979). Pollination is by
insects, usually bees.
Picture-2: Leaves of Jatropha curcas plant growing at PMY, Karachi.
A study by Raju and Ezradanam(2002) indicates that pollen is deposited in sufficient amount,
which is visible by its yellow colour even to the
naked eye. However, the plant with predominant
xenogamy requires mostly xenogamous pollen for
more fruit set, after selective elimination of
growing fruit. Therefore, pollen transfer between
con specifics has a great bearing on the net
percentage of natural fruit set.
Fruits are 2.5 4 cm long (picture-4),
finally drying and splitting into 3 valves, all or
two of which commonly have an oblong black
seed (picture-5) as also reported by Morton, 1977
and Little et al., 1974.
Picture-3: First flowering of J.curcas plant at CARS, Farm Karachi.

37

Annexure B

Picture-4: Bunch of J. curcas fruits (1st bearing).

Picture-5: Dried & de-hulled fruit and seeds of J. curcas.

During storage seeds loose moisture that after 25 days dropped to 0.650 grams compared to
0.910 g on the 2nd day of harvest and de-hulling.
Pest and Diseases: J. curcas plantation at PMY was attacked by white fly, leaf minor and roller
and mealy bugs. Whitefly was controlled by simple water and water + detergent spray. No egg
mass or nymph was observed on or under the leaf surface. Leaf minor and roller were treated
with emulsified diesel with a success rate of 50--70 %. J. curcas was observed to be quite
attractive to mealy bug and a heavy attack was noticed with repeated instances both in field and
nursery plantation. Initially Cypermethrin was applied in the nursery area but the results were not
satisfactory as resurgence was also observed. Therefore, a new test combination of Neem and
Gliricida sepium leaves decoction was sprayed on to infested plants. Spray of test material for
three weeks (once every week) reduced and dried the mealy bugs. However, this was only a
crude test and a proper study is required to work out the efficacy of the test material against
mealy bugs. The mealy bug species found on J. curcas was of special interest and concern.
Though, on microscopic examination it appears more like Papaya mealy bug and not the one that
has been a menace in the cotton fields (Picture-6). Identification of exact species is under
investigation.

38

Annexure B

The percent oil content of three different coded seed samples sent to P.C.S.I.R.
Laboratories reported 28.71%, 35.% and 30.53% oil from seeds of PMY, CARS and M/S Isons
of
Pakistan(imported seed), respectively (table-4). It is encouraging that both the local samples have
low free fatty acid (FFA) content 0.54% compared to 6.57% recorded in imported seed, a
positive sign that would reduce the trans-esterification cost in the preparation of biodiesel.
Biodiesel consists of alkyl esters of fatty acids produced by the trans-esterification of
vegetable oils. M.A. Fangrui and M.A. Hanna (1999), A.K.Agarwal and L.M.Das (2000) and D.
Agarwal et al, (2007) have narrated the conversion procedure of plant oil into biodiesel. While
Rao et al (2008) carried out experimental investigations of Pongamia, Jatropha and Neem Methyl
esters as Biodiesel on C. I. Engine indicating that B20 have closer performance to diesel and
B100 had lower brake thermal efficiency mainly due to its high viscosity compared to diesel.
However, its diesel blends showed reasonable efficiencies, lower smoke, CO and HC. Pongamia
methyl ester gives better performance compared to Jatropha and Neem methyl esters (Rao et al,
2008).
Preparation of Biodiesel in PSO labs and results of related ASTM protocols are given in
table . The experience of complete life cycle of biodiesel production, blending and usage
promise economic viability of J. curcas oil based biodiesel in the country. The effects of
Biodiesel (B5) observed on engine performance of field vehicle (picture-7) and exhaust emission
were found quite satisfactory. A comprehensive Analysis of Biodiesel impacts on Exhaust
Emission is available on www.epa.gov/otaq/models/analysis/biodsl/p02001.pdf.

Conclusion: The Jatropha cultivation experience since 2007 till now has increased the confidence
level towards adoption of this exotic species in the country. Parallel experiences of some local
farmers growing Jatropha at their farms/sites have also been encouraging so far. However, being
exotic species regulations should be framed simultaneously to permit import of seeds of good
quality, known origin and proven tract record if commercialized Jatropha plantation is to be
addressed for bio-diesel BD-5 or BD-10 blends in the country for reaping the benefits of Carbon
sequestering /Carbon credit. Opportunities for new investments, jobs, environment improvement
and reclamation of degraded soils do exist but could not be expedited till proper legal frame
work is drafted, approved and implemented. The
current
agriculture
practices,
resources,
agribusiness and exports could not be endangered
if not jeopardized by ignoring the quarantine and
pest risk analysis and other relevant studies. But
till such time contained cultivation on
government/selective lands is required for
carrying out various experiments particularly from
yield to harvest and processing levels.

Picture-7: Dedicated field vehicle of PSO running on B-5 for over one year now.

39

Annexure B

References:
1. FAO (2008); Forestry and Energy Key issues; FAO Forestry Paper 154, pp 73, Rome.
2. Dr Rachel Smolker Brian Tokar, Anne Petermann, Eva Hernandez (2007); The real cost of
agrofuels: Current and Future Technologies for Agrofuels: Path to Deforestation, Climate Change
and Loss of Livelihoods", pp 74, pdf .
3. H. S. GINWAL1,*, S. S. PHARTYAL1, P. S. RAWAT2 and R. L. SRIVASTAVA3 (2005) Seed
Source Variation in morphology, Germination and Seedling Growth of Jatropha curcas Linn. in
Central India, pp. 76-80, Silvae Genetica Vol. 54, No. 2.
4. Raju, A. J. S. and V. Ezradanam (2002); Pollination ecology and fruiting behaviour in a
monoecious species, Jatropha curcas L. (Euphorbiaceae); Research Communication, pp. 13951398 CURRENT SCIENCE, VOL. 83, NO. 11.
5. MA Holl, MB Gush, J Hallowes+ and DB Versfeld (2007); Jatropha curcas in South Africa: An
Assessment of its Water Use and Bio-Physical Potential; pp. 154, WRC Report No. 1497/1/07,
ISBN 978-1-770050593-3
6. BGIR/BSI Database (2004); Database of Oil Yielding Plants, Botanic Garden of Indian Republic
(BGIR), Botanical Survey of India (BSI), Last updated on April 5th, 2004.
7. Becker, K. and Makkar, H.P.S. 2000. Jatropha and Moringa. Available from < http://www.unihohenheim.de/~www480/docs/se990720/jatropha.htm >[Last Update Used: May 11, 2000].
8. Begg,
J.
and
Gaskin,
T.
1994.
Jatropha
curcas
L.
Available
from
<http://www.inchem.org/documents/pims/plant/jcurc.htm > [Last Update Used: June, 1994].

9. Biodiesel S.A. 2004. Specialising in producing environmentally friendly fuel and methyl esters.
Available from < http://www.biodieselsa.co.za >[Last Accessed: July 1, 2004].
10. BUYSOMALI, 2003. Jilbadig Tree (Jatropha curcas) natural fencing with real income
potential.
Available
from
<http://www.buysomali.com/natural
_products_jatropha_business_opport unities.htm > [Last Update Used: December 5, 2003].
11. Dehgan, B. and Webster, G.L. 1979. Morphology and infrageneric relationships of the genus
Jatropha (Euphorbiaceae). University of California Publications in Botany, Vol. 74.
12. Duke,
J.A.
1983.
Handbook
of
energy
crops.
Available
from:
<http://www.hort.purdue.edu/newcrop/duke_energy/Jatropha_curcas.html > [Last Update Used:
January 7, 1998].
13. Duke, J.A. and Ayensu, E.S. 1985. Medicinal plants of China. Reference Publications, Inc.
Algonac, MI.
14. Duke, J.A. and Wain, K.K. 1981. Medicinal plants of the world. Computer index with more than
85,000 entries. 3 vols.

40

Annexure B

15. Francis, G., Edinger, R and Becker, K. 2005. A concept for simultaneous wasteland reclamation,
fuel production, and socio-economic development in degraded areas in India: Need, potential and
perspectives of Jatropha plantations. Natural Resources Forum, 29: 1224.
16. From biomass NREL leads the way to Biofuels(2006); A brochure NREL/BR-510-39436, pp.6,
pdf. http://www.nrel.gov/biomass/
17. Daghar, JC, Bhagwan, H and Kumar, Y (2004). Seed germination studies of Salvadora persica
and Jatropha curcas, Indian Journal of Forestry 27 (3): 283289.
18. D. Agarwal, L. Kumar, A.K. Agarwal (2007) "Performance Evaluation of a Vegetable oil fuelled
CI Engine". Renewable Energy. Vol. 33
19. M.A. Fangrui,,M.A. Hanna , "Biodiesel production: A review", Bio Source Technology, Vol.70,
1999, 1-15.
20. A.K.Agarwal, L.M.Das, "Biodiesel development and characterization for use as a fuel in
C.I.Engine", Journal Of Engineering, Gas Turbine And Power (ASME), Vol.123, 2000, 440-447.
21. T. Venkateswara Rao, G. Prabhakar Rao and K. Hema Chandra Reddy (2008), Experimental
Investigation of Pongamia, Jatropha and Neem Methyl Esters as Biodiesel on C.I. Engine,
Jordan Journal of Mechanical and Industrial Engineering. Volume 2, Number 2 (ISSN 19956665)

41

Annexure B

Table-1: Seed germination as recorded at CARS Farm, PARC, Karachi.

S.
No.
1
2
3
4
5

Source of seed/
receiving date
IABGR/NARC
14/2/07
India (PSO)
PSO/Thailand
1/4/08
PSO/Thailand
2/4/08
K.U/USA
/4/08

No.
of
seeds
20

Date of sowing
Pots
field

Germination
Date
Days No.

Germination
Pots field
%
30
20

1/3/07

1/3/07

14/3/07

13

259
106

17/5/08
17/5/08

19
5-9

4
33

31

32

17/5/08

5-7

21

66

25

25/5/08

Table-2: Growth parameters recorded at CARS/PARC Farm, Saleh Muhammed Goth, Karachi

S . S.
S. No.
1
2
3
4
5
6
7

Vegetative/ generative
parameters recorded

Jatropha curcas L. plant

2
3
4

Mean

5
Plant height (cm)
Stem diameter (cm)
Number of branches
Length of branches (cm)
Capsule weight (grams)
Number of seeds/capsule
1000 fresh seed weight (g)

275
25
8
186
5
3
1000

264
27
5
125
4.3
2-3
850

42

335
45
52
340
5.1
3
925

275
27
20
180
4
3
900

257
20
12
180
4.4
3
875

281.5
28.8
19.4
202.2
4.56
3
910

Annexure B

Table-3: Soil Analysis Report of PMY (Site) carried out by soil testing laboratory Thatta
Date of Sample rec.:
Date of Analysis:
Sample Received by:
Thatta
Area:
Karachi

Lab.
No.

Field No.

Texture

pH

Electrical
Conductivity
(dS/m)

Available
Phosphorus
(P) (ppm)

2161 S-1 (0-6) Loam 8.5

1.0
4.0
2162 S-1 (6Loam 8.4
1.0
1.6
12)
2163 S-1 (12Loam 8.7
1.0
1.6
18)
2164 S-2 (0-6) Loam 8.4
1.2
1.6
2165 S-2 (6Loam 8.4
1.2
1.6
12)
2166 S-2 (12Loam 7.3
1.0
1.6
18)
2167 S-3 (0-6) Loam 7.3
1.4
9.2
2168 S-3(6-12) Loam 7.5
1.3
2.4
2169 S-3(12Loam 7.4
1.3
5.6
18)
Key:
pH:
Neutral 6.5-7.5,
Electrical Conductivity (dS/m): Normal < 4.0
Available Phosphorus (ppm):
Poor <7.0
Available Potassium (ppm):
Poor <125
Sodium Adsorption Ratio (SAR): Normal < 10.o

Available
Potassium
(K) (ppm)

22 September 2008
26 September 2008
MDO

Soluble @
Exch. Sodium
(Na) (meq/L)

Soluble
Ca+Mg
(meq/L)

Sodium
Adsorption
Ratio
(SAR)

140
160

1.7
1.7

19
15

0.6
0.6

140

6.5

10

2.9

300
140

7.8
7.8

10
10

3.5
3.5

100

8.3

10

3.7

460
180
260

1.7
5.7
6.1

10
10
11

0.8
2.5
2.6

Alkaline > 8.5

Saline > 4.0
Medium 7.0-13
Medium 125-250
Sodic > 10.0

Satisfactory >13
Satisfactory >250

Table- 4: Comparative Analysis of Seed Oil content from three different sources

S. No. Sample Source

1
2
3

PMY, PSO, Karachi

CARS Farm, PARC,
Karachi
M/S Isons

Parameters tested using AOCS Method 2004

Moisture %
Oil content %
FFA (% as
OA)
6.56
28.71
0.54
6.45
35.36
0.54
6.83

30.53

43

6.57

Annexure B

Mealy bug on

Mealy bug on

Picture-6: Comparison of Mealy bug found on cotton and Jatropha in Karachi with
Papaya Mealy Bug from USDA-CSREES Integrated Pest Management Centers in
cooperation with the National Plant Diagnostic Network, APHIS, the National Plant Board,
and the Land Grant Universities.

44