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43
Open Access
Amala Cancer Research Centre, Amala Nagar, Thrissur 680 555, India
1874-3404/10
44
Tharakan et al.
45
Normal
RGM
1.5g/kg b.wt
0.75g/kg b.wt
7992 372
7917 231
7767 361
Haemoglobin (mg/dl)
10.53 1.35
10.67 1.39
11.06 2.05
0.05 0.03
0.08 0.02
0.15 0.25
0.01 0.005
0.03 0.26
0.02
SGPT (U/L)
44.21 8.44
66.8 9.1
53.5 10.03
SGOT (U/L)
130.7 19.91
141.1 21.8
142.9 13.5
ALP (U/L)
195.25 26
318.31 88
259.8 33.5
Albumin (mg/dl)
3.85 0.36
3.95 0.3
3.98 0.35
Globulin (mg/dl)
2.7 0.35
2.97 0.36
3.7 0.4
Total protein
6.55 0.24
6.91 0.36
6.68 0.56
BUN (mg/dl)
19.65 1.74
22.7 1.87
25.3 3.05
Creatinine (mg/dl)
0.38 0.04
0.4 0.06
0.41 0.08
Sodium (mEq/L)
153.5 1.5
150.33 3.55
151.7 3.14
5.24 0.95
Potassium (mEq/L)
5.43 0.46
5.53 0.68
Chloride (mEq/L)
78.5 14.41
86.66 22.4
80.7 21.1
Bicarbonate (mEq/L)
15.41 2.24
27.55 3.74
38.66 23.8
*p<0.05, *p<0.01.
Fig. (2). Effect of chronic administration of Rasagandhi mezhugu (RGM) on body weight.
Normal
Rasagandhi mezhugu 0.75g/Kg body weight
Rasagandhi mezhugu 0.325g/Kg body weight
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Tharakan et al.
Fig. (3). Effect of chronic administration of Rasagandhi mezhugu (RGM) on food consumption.
Normal
Rasagandhi mezhugu 0.75g/Kg body weight
Rasagandhi mezhugu 0.325g/Kg body weight
was no significant change of haemoglobin content and differential counts. There was no significant alteration in hepatic
and renal function parameters. Chronic administration of
these drugs did not produce any alteration in sodium,
potassium, chloride and bicarbonate levels (Table 2).
No remarkable histopathological changes were noted in
the internal organs of rats receiving these drugs (Figs. 4, 5).
Normal
3
6392 498
RGM
0.75g/kg b.wt
8083 422
0.325g/kg b.wt
7633 225
11.98 2.85
10.15 2.34
12.35 1.0
0.32 0.22
0.3 0.15
0.42 0.29
0.26 0.36
0.42 0.29
0.11 0.05
SGPT (U/L)
72.8 10.5
88.8 14.1
74.83 13.38
SGOT (U/L)
176.4 31.6
176.3 17.26
143.2 17.65
ALP (U/L)
419.8 94.1
493.8 142.5
467.7 154.3
Albumin (mg/dl)
3.63 0.42
3.42 0.56
3.62 0.52
Globulin (mg/dl)
4.03 0.19
4.03 0.36
4.00 0.39
Total protein
7.66 0.28
7.45 0.42
7.62 0.52
BUN (mg/dl)
66.58 7.45
56.13 6.6
63.26 7.48
Creatinine (mg/dl)
0.46 0.05
0.38 0.04
0.38 0.04
Sodium (mEq/L)
148 3.43
148.8 2.92
150.3 7.6
Potassium (mEq/L)
5.15 0.38
5.2 0.49
4.9 0. 53
Chloride (mEq/L)
101 3.28
105 2.28
102.8 5.07
Bicarbonate (mEq/L)
31.0 3.68
34.6 2.5
32.3 3.88
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Fig. (4). Histopathology of liver, kidney and intestine after chronic administration of Rasagandhi mezhugu (RGM).
a) Normal liver, b) Treated liver (Rasagandhi mezhugu 0.75g/Kg body weight)
c) Normal kidney, d) Treated kidney (Rasagandhi mezhugu 0.75g/Kg body weight)
e) Normal intestine, f) Treated intestine (Rasagandhi mezhugu 0.75g/Kg body weight)
Liver tissues showed normal structure. Portal area and central venous system appeared normal. Hepatocytes showed
normal morphology. Kupffer cells and sinusoidal spaces
were normal.
Kidney showed normal glomeruli. Renal tubes were
normal. Interstitial tissues also appeared mild oedema. No
other specific lesion seen. Intestine showed normal villi and
mucosal glands. Mucosa, submucosa and muscularis mucosa
did not show any specific pathological lesion.
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Tharakan et al.
Fig. (5). Histopathology of brain, spleen and thymus after chronic administration of Rasagandhi mezhugu (RGM).
a) Normal brain, b) Treated brain (Rasagandhi mezhugu 0.75g/Kg body weight)
c) Normal spleen, d) Treated spleen (Rasagandhi mezhugu 0.75g/Kg body weight)
e) Normal thymus, f) Treated thymus (Rasagandhi mezhugu 0.75g/Kg body weight)
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Copper
Mercury
Lead
Arsenic
Normal liver
0.14 0.53
Nil
4.4 7.6
77.06 21.14
Treated liver
7.87 1.8***
44.2 19.14**
8.1 8.89
124.06 138.1
Normal kidney
8.4 1.9
4.2 7.3
5.6 5.7
426.2 117
Treated kidney
36.2 11.15**
151 38***
10 2.9
636.8 289.7
Normal brain
8.4 3.38
56.2 44.97
15.6 7.7
832.4 133
Treated brain
7.2 3.6
169.9 110.67***
10.6 5.5
978.9 449
Normal blood
0.5
Nil
1.3 0.8
467.3 26.81
Treated blood
1.21 0.3
58.5 46.23**
2.2 1.5
458.3 110.04
Normal urine
1.1
Nil
2.1
477
Treated urine
0.95
11
2.4
315
Clinical Parameters
Pretreatment
Body weight
48.0 8.6
51.52 9.2
4.08 0.41
3.48 0.76
Haematocrit (%)
32.35 3.57
31.95 6.63
Haemoglobin (mg/dl)
11.93 4.75
11.3 5.52
167.91 58.31
151.91 51.5
WBC count x 10 /l
6.98 1.55
5.86 1.42
SGOT (U/L)
20.54 7.25
21.91 13.06
SGPT (U/L)
8.00 3.01
16.14 11.71**
ALP (U/L)
58.35 16.55
50.22 29.0
Urea (mg/dl)
17.89 5.0
22.84 5.95**
Creatinine (mg/dl)
0.71 0.14
0.71 0.12
Globulin (mg/dl)
4.03 0.19
4.03 0.36
Total protein
7.66 0.28
7.45 0.42
Sodium (mEq/L)
139.95 7.20
141.27 5.11
Potassium (mEq/L)
4.75 0.86
4.32 0.53
Platelet count x 10 / l
3
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Tharakan et al.
Analysis of the rat tissues of heavy metal content indicated that the administration of RGM produced significant
increase in the level of mercury in liver, kidney, brain as well
as in the urine. Similarly copper content was very high in
liver and kidney of treated rats. The levels of lead and arsenic did not show significance compared to normal. Sidha
practitioners claim that many of their preparations containing
heavy metals do not produce any toxicity to internal organs.
However, the accumulation of mercury and copper in the rat
tissues may indicate that long term intake of Sidha preparations can produce toxic symptoms in the patients. Interestingly administration of RGM even after one year in HIV
patients did not alter the haematological and serum parameters and these patients did not show any signs of toxicity.
[2]
[8]
Usefulness of Sidha medicines in many ailments including HIV has been shown proved beyond doubt. However
mechanism of action of these drugs is not understood properly. Since the Sidha preparations always use high heat to
produce bhasmas (ash) [14]. It is possible that the ingredients
present in these preparations can be very much different
from the original drugs. Absorption and distribution of these
drugs have not been clearly understood. In the present study,
indicate that even though RGM does not produce any
Received: April 05, 2010
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[4]
[5]
[6]
[7]
[9]
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[14]