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Abstract - This paper assesses the global and sectoral implications of the growing demand for bio-based inputs for energy and
fuel production. This paper also pinpoints the importance of relative prices between bio-based and fossil inputs in the petroleum
and electricity sector and current advances in the production of biofuel. First generation biofuels continue to be substantially
subsidized, and this has contributed to the increasing use of such fuel. This report reviews the current status of second and third
generation biofuels. Second generation biofuels are made from cellulose, which is in more abundant supply than the first
generation biofuel feedstock. Whereas third generation biofuels have become an innovative alternative that offers a wide variety
of exceptional benefits. Nowadays the production of biodiesel from microalgae is an option that has attracted strong interest of
the scientific community and should be evaluated to determine the technical, technological, economic and environmental
sustainability of the process. Industrial biotechnology with its competitive, clean and clever use of bio-based technologies can
play a key role in making biofuels more sustainable.
Keywords - Biofuel, Fossil Fuels, Biomass Feedstock, Cellulose, Microalgae
1. Introduction
Each and every solution is crucial to shift towards future with
a sustainable energy resources and healthy goods production.
Hence the energy source has to be more efficient and
processes for the production of sustainable energy resource
have to be improved with complete new technologies.
Biotechnological applications with its clean and chicanery use
of bio-based technologies can play a vital role in making
biofuels more sustainable [1]. Fossil fuels are hydrocarbons,
primarily coal, fuel oil or natural gas, formed from the
remains of dead plants and animals. Fossil fuels because of its
high heating potential, availability and ignition properties and
it has been used as a source for transportation and other
energy purposes [2]. Due to speedy fossil fuel depletion, the
global warming, rising future energy requirement and demand,
and climatic changes because of emission of the fossil fuel,
we are forced to search for alternative source of energy.
Among various alternative sources available today, biofuel is
the one of the best alternative source of energy for
diminishing dependency on fossil fuel by replacing it fully or
partially [3]. Through sustainable biofuel production and
continuous speedy improvements in both bio feedstock and
processes for biofuel production, it is possible to reduce the
dependency on non- renewable fossil fuels and to provide
national energy security [4]. Biofuel is any solid, liquid or
gaseous fuels derived from organic biomass which is any
living matter such as field crops, wood products, water plants,
2. History
Ever since human discovered fire, charcoal, woodchips and
cattle dung have been used as a source of energy and still
today people used these solid fuels for heating and cooking in
many parts of the world. In mid 1700s and early 1800s, Oil
extracted from whale was broadly used for lighting purposes
[7]. From more than a century, biofuel has been around us
despite the term biofuel came into existence few years back.
Rudolph Diesel in late 19th century used peanut oil to generate
power and he is the one who started using vegetable oil for the
production of energy source. Diesel also developed first
working engine that runs on peanut oil at the Worlds
Exhibition in Paris in 1900 [8, 9]. Henry Ford was also an
initial proponent of biofuel and he developed the Model T car
in 1903 which was totally designed to use hemp derived
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that is, they convert solar energy into chemical forms through
photosynthesis. Their photosynthetic mechanism is
comparable to land based plants, but due to a simple cellular
structure, and their underwater habitat, where they have
efficient access to water, CO2 and other nutrients, they are
generally more efficient in converting solar energy into
biomass [23]. Microalgae have a very good prospective as
biodiesel precursors since many of them are very rich in oils,
at times with oil contents over 80% of their dry weight, even
though all species are not suitable as biodiesel production oils
[24]. There are many advantages of using microalgae as a
source of biofuel production. They can double their biomass
in less than 24 hours. Additionally they can be grown in
wastewater, or any non-potable water. The synthesis of
microalgae biodiesel could be integrated with the CO 2
removal from power generation facilities for waste water
treatment from which microalgae would remove NH 4+, NO3and PO43-[25]. However, there are certain limitations in using
microalgae for synthesis of biofuel. Microalgae
biomass-based biofuel have several problems which include
the optimization of high density and large surface units of
production. The location of the microalgae production unit
may also pose a difficulty [26].
4. Application of Biotechnology in
Biofuel Production
Biotechnology uses eye-catching way of producing biofuel
which increases the yield without much increase in the energy
needed for production. In the past few decades, significant
improvement has been made with the help of molecular
biology so as to improve the microbial activity and enzymes
[30]. The use of Genetically Modified Organisms (GMOs) is
found to be most efficient and quick method to improve
biofuel conversion, particularly in case of lignocellulosic
biomass [31]. With the help of biotechnology, structure of cell
wall and composition of lignocellulosic in plant cell can be
modified to enhance ethanol yield per acre [32].
Biotechnology can influence yield density by varying plant
physiology, their architecture, along with their photosynthetic
efficiency and it has also shown its ability to lessen agronomic
inputs for instance herbicide and pesticides. Advancement is
rapidly being made on characters which enable crops to take
up and consume nutrients more resourcefully, thus equipping
them to be grown with less amount of fertilizer. Producing
biomass crops on supposed marginal acres, such as land
that is highly dry, or with deprived soil characteristics, can
raise the scale of biofuel production without any influence on
food production acres. Biotechnology is focusing on the
development of drought, cold, salt and heat plants as well as
plants that can survive on a wide range of soil conditions. For
a biomass feedstock plant, a higher level of cellulose and
hemi-cellulose content would give better fermentation yield
and hence gallons of ethanol per ton of biomass. This results
in added net energy per acre and more revenue. Research has
already been carried out successfully for the cloning of genes
that code for cellulases and polygalacturonase enzymes to
develop low-cost effective biorefinery strategy to achieve
maximum biomass conversion and improved gas
chromatography-mass spectrophotometry method that has
been developed by researchers at TAMUK in understanding
the catalytic action of the expressed enzymes in the
bioconversion process [33]. Difficulties faced by the biofuel
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5. Conclusions
According to Current state-of-the-art knowledge, Green
House Gas can be abridged within the next 30-40 years and
the demand for low carbon dioxide emission fuel in all forms
of automobile is enormous. Because of fast growing price of
crude oil with several adverse effects on environment, the
demand for the biofuel production has increased. Liquid
biofuel such as bioethanol, biodiesel and gaseous biofuel such
as biomethane, biohydrogen have developed as an effective
alternative to the fossil fuel resource. To reduce the
dependency on fossil fuel, biofuel was found to be most
efficient alternative among various existing source of energy.
Biofuel can replace the effective use of fossil fuel which is
known reason for the greenhouse effect. Researches have
shown that first generation biofuel that is crop biofuel has a
durable influence on agriculture world-wide. Second
generation biofuels are generally made from non-crop
biomass by two different fundamental approaches
(biochemical and thermochemical second generation biofuel).
Cellulose because of its easy availability, price and ability to
be proficiently degraded by cellulolytic bacteria, it has
become an eye-catching biofeedstock for the production of
second generation biofuel. A wide range of agricultural and
industrial residues, from lignocellulosic forestry and domestic
waste can be used as precursors of biofuels with the help of
microbial enzymes. Biofuels production plant design,
heterogeneous catalysts and enzyme immobilization
techniques, protein engineering of lipases, alcohol
dehydrogenases or hydrolases to increase their activity and
reusability, genetic engineering of microbes to facilitate both
the pretreatment of precursors, and the synthesis and
purification of the biofuels. Biotechnology plays a crucial role
in biofuel production. It helps in decreasing agronomic inputs,
in optimizing process characteristics and in engineering the
plants to produce high yield and hence high energy. With
progresses in the field of biotechnology, improvements in
synthesis of biofuels can be expected.
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