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hypertensive rat
Adenosine has physiological functions;
1. Therapy neonatal refractory pulmonary hypertension.
Ardiansyah, Hitoshi Shirakawa, Takuya Koseki1, Michio Komai
2. Attenuates proliferation in both human and rat glomerular
mesangial cell.
3. Decrease in blood glucose and insulin level after oral
Adenosine administration.
9 10 11 12 13 (Week) 1.2
Insulin (ng/mL)
160 *
* *
one week 140
0.8
acclimation 1.SHRSP administrated by 120
distilled water as control 100
group and adenosine (10 1.Sacrificed 0.4
80 Control Adenosine
mg/L) as adenosine 2.Blood and organ
OGTT after 16 h fasting; group. collecting for further 60 0.0
analyses 0 30 60 120 0 30 60 120
Control (1 mL 20% glucose 2.Water intake/food intake
solution) Time after administration (minutes) Time after administration (minutes)
(F2 diet; Funabashi Co.)
Adenosine (1 mL 20% measured every day. iAUC glucose iAUC insulin
glucose solution containing †
3.Systolic blood pressure 6000
40 mg/mL adenosine) * Control
and body weight 5000 50
iAUC glucose
4000
30
3000
20 means + SE, n = 4
2000
The rats were housed individually in stainless steel cages under a controlled atmosphere 10
1000 * (P<0.05)
(temperature, 23 + 2 oC; humidity 50 + 10 %; 12 h light-dark cycle, 08:00 am-08:00 pm) 0 †
0 (P<0.01)
Growth parameter
Systolic blood pressure
Water intake
water intake (mL/day)
10 † 4
180 † 3
0
Control Adenosine
2
3 140 1
Relative organs weight Control Adenosine 0
relative weight (%)
Control Adenosine
2 * 100
10 11 12 13
means + SE, n = 4
age (weeks)
1 * (P<0.05) †
* (P<0.05) (P<0.01); means + SE, n = 4
0
Liver Kidney Heart Epididymal fat
1
80 Plasma lipid levels Plasma glucose
* Plasma FFA Plasma insulin
* † 200 †
0.4
Plasma lipid (mg/dL)
60 4 *
Glucose (mg/dL)
160
Insulin (ng/mL)
FFA (mEq/L)
0.3
* 120 3
40
0.2 2
80
* 0.1
20 40 1
0.0 0 0
0
TG TC HDL-Cholesterol LDL-Cholesterol Plasma leptin Plasma adiponectin
70 *
* 12
Adiponectin (μg/mL)
3
Liver lipid (mg/g tissue)
Leptin (ng/mL)
9
50 Control Adenosine 2
40 6
* †
30 means + SE, n = 4 1 3
†
20 * (P<0.05) (P<0.01)
0 0
10
0 * (P<0.05) † (P<0.01); means + SE, n = 4
Control Adenosine
Total lipid TG TC
4
Blood plasma (mg/dL)
220 *
160
NO (μmol/L)
3 †
200 120
2 80
180
† * † means + SE, n = 4
1 40
160 Control Adenosine †
* (P<0.05) (P<0.01)
0 0
140 TG TC Glucose
0 1 2 4 6
†
Time after administration (hour) Control Adenosine * (P<0.05) (P<0.01); means + SE, n = 4
Conclusion
Adenosine
Insulin sensitivity
&
Adiponectin level
Improvement Improvement
Blood pressure
glucose metabolism lipid metabolism