horizontally to stimulate the gravitropic response, the generation of ROS is detected in the apex. Production of ROS is asymmetric and occurs in the lower cortex. The production of ROS is responsible for the gravitropic response because the gravitropic response is inhibited by treatment with ROS scavengers, such as N-acetyl cysteine (NAC). Second, the application of auxin to roots stimulates ROS production in root cells in planta and in root protoplast cultures. Further indication of a directional causeeffect relationship between auxinROS and gravitropism comes from roots treated with N-1-naphthylphthalamic acid (NPA, an inhibitor of auxin transport). NPA treatment results in inhibition of gravitropism. Adding H2O2 can reverse the effect of NPA. Joo et al. indicate that ROS might work by activating kinase, but they also take into account that other mediators of
The role of auxins in gravitropism was
discovered during early molecular research into phytohormones. Further research led to the conclusion that auxin re-distribution is responsible for the gravitropic response in roots. This auxin effect, first proven in monocots was found later in dicots, including Arabidopsis. In a recent article, Jung-Hee Joo and colleagues1 investigated the role that reactive oxygen species (ROS) have in this process in the monocot Zea mays. Their results strongly suggest that ROS are key mediators of the auxin effect in gravitropism. ROS are key mediators of the auxin effect in gravitropism. First, Joo et al. show that ROS are produced following the gravitropic stimulus. When maize roots are placed
the gravitropic response might exist,
such as calcium and inositol (1,4,5)-trisphosphate. The generation of ROS has already been identified in several plant processes, in particular, their involvement in defence responses. Recently, calcium-dependent protein kinases have been reported to be important in plant defence responses. In recent years, we have witnessed the convergence of transduction chains from diverse plant hormones towards common points. A question provoked by Joo et al.s work is: do other plant hormones apart from auxin have ROS as mediators of their action? 1 Joo, J-H. et al. (2001) Role of auxin-induced reactive oxygen species in root gravitropism. Plant Physiol. 126, 10551060
Emilio Cervantes ecervant@gugu.usal.es
Plant SEC14-like proteins controlling a phosphoinositide traffic jam
Phosphatidylinositol transfer proteins (PITPs) are defined by their ability to transfer phosphatidylinositol or a phosphatidylcholine monomer between membrane bilayers in vitro. Studies in mammalian systems have suggested that PITPs play a key role in controlling the activities of various inositol lipidsignalling pathways by regulating the production of some phosphoinositides. These inositol-containing phospholipids belong to a large and heterogeneous group of lipids with structural and key regulatory roles in a wide range of cellular signalling events. Recently, several plant proteins similar to yeast PITP (SEC14) have also been identified and characterized. Now, Phillip Kapranov et al.1 report an important insight into the role of PITP in nodule development in Lotus japonicus. With the objective of identifying new sequence tags correlated with late stages of symbiotic root nodule development, the group previously characterized a novel nodule-specific cDNA, LjNOD16. Now, they report that the predicted Nlj16 amino acid sequence might represent a truncated version of a much longer protein. They http://plants.trends.com
report an unanticipated relationship
between Nlj16 and the predicted protein products of four members of a previously undescribed gene family that encode novel PITP-like proteins. All the members of this protein family share a two-domain structure consisting of an N-terminal PITP-like domain and a C-terminal domain with a high level of homology to nodulin Nlj16 that is thought to work as a specific plasma membrane-targeting domain. LjNOD16 expression in nodule tissues results from an unusual transcriptional control that is modulated by a nodule-specific promoter located in an intron of the LjPLP gene. key role for Nlj16 as a dominant negative effector that can control the expression of specific PITPs in nodules. Kapranov et al. have identified the presence of a novel family of developmentally regulated genes encoding SEC14-like proteins (LjPLP) and suggest a new key role for Nlj16 as a dominant negative effector that can control the expression of specific PITPs in nodules. These data suggest a new scenario where
LjPLPs might promote phosphoinositidedriven signalling cascades initiated at the
plant cell plasma membrane. Because the putative down regulation of LjPLP function in nodules might be relevant to the symbiosis between legumes and their endosymbionts, it would be important to uncover the range of responses involving LjPLP genes. Does PITP perform various functions using different molecular mechanisms? Does LjPLP over-expression affect nodule development? Kapranov et al. have identified a new family of PITPs that have the potential to modulate inositol lipid-signalling pathways involved in the process of symbiosis between legumes and their endosymbionts in Lotus japonicus, an important step in understanding how plants coordinate developmental processes related to symbiotic nitrogen fixation. 1 Kapranov, P. et al. (2001) Nodule-specific regulation of phosphatidylinositol transfer protein expression in Lotus japonicus. Plant Cell 13, 13691382
Joaqun Medina medina@inia.es
1360-1385/01/$ see front matter 2001 Elsevier Science Ltd. All rights reserved.
Differential Effects. Differential effects of coinoculations with Pseudomonas jessenii PS06 (a phosphate-solubilizing bacterium) and Mesorhizobium ciceri C-2/2 strains on the growth and seed yield of chickpea under greenhouse and field conditions