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CONFIDENTIAL
The glucose isomerase activity in the last six 30-gallon
fermentations has ranged from 860 to 1,060 uU per ml . This is a sub-
stantial increase in activity and should reduce the enzyme production
costs . The fermentation series this week should give us enough enzyme
for the pilot run in Cedar Rapids .
be set up in series so that maximum flow rate data can be obtained . The
optimum temperature is 60-62°C and the column temperature must be closely
regulated . At 70°C, an8% increase in conversion is obtained, but there
is a 33% reduction in enzymatic activity .
CGP :msc
cc : Mr . D . S . Peoples
Dr . Murray Senkus
Dr . S . .O . Jones
Dr . C . E . Teague, Jr .
~D£ E . D . Nielson
Dr . R . E . Brouillard
Dr . E . C . Ritchell
Mr . W . C . Black
Mr . Claude Sawyer
Dr . D . J . Lartigue
Dr . J . L . Ayres
Dr . Paul Benko
Mr . L . E . Hayes
Dr . Chin K . Lee
Miss Margaret E . Long
i
Dr . C . W . Nystrom . I
Dr . W . S . Simmons