PDF 01

YMC GENERAL CATALOG
Reversed-Phase
C18 Columns (ODS)
Types and characteristics of C18

packing materials
14
Guides for selecting C18 packing materials
15
YMC-Triart C18
1623
YMC-UltraHT series
YMC-UltraHT series
YMC-UltraHT Pro C18
YMC-UltraHT Hydrosphere C18
24,25
26
27
Pro series
Features of Pro series ODS columns
YMC-Pack Pro C18
Hydrosphere C18
YMC-Pack Pro C18 RS
2831
3235
3639
4043
YMC-Pack ODS series

YMC-Pack
YMC-Pack
YMC-Pack
YMC-Pack
ODS-A
ODS-AM
ODS-AQ
ODS-AL
44,45
46,47
48,49
50,51
,
J sphere ODS series
ODS-H80M80L80
52,53
YMC-Pack PolymerC18
54,55
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Types and characteristics of C18 packing materials

Octadecyl-silica (ODS) is the industry standard packing material for HPLC applications. YMC Co., Ltd. offers packing materials with an impressive
assortment of functional groups for liquid chromatography and a selection of ODS columns that is far ahead of all others in variety. We also offer
hybrid-silica based C18 packing materials that are widely applicable to a variety of fields, from microanalysis to large-scale isolation.
Types of C18 packing materials

Base
Products
Hybrid silica
ODS
Reversed-Phase C18 Columns
YMC-Triart C18
Silica
YMC-UltraHT series
YMC-Pack Pro C18
Hydrosphere C18
YMC-Pack Pro C18 RS
YMC-Pack ODS series
J
sphere ODS series
Polymer
YMC-Pack PolymerC18
Types and characteristics of C18 (ODS) packing materials

Product name
ODS
Pore Particle
Usable
size
size
Silanol pH range
m C% treatment
YMC-Triart C18
120
20
Yes
5
120
16
Yes
Hydrosphere C18 120

Pro series
Pro C18
120
Hydrosphere C18 120
Pro C18 RS
YMC-Pack ODS series
80
120
ODS-A
200
300
ODS-AM
ODS-AQ
120
120
200
ODS-AL
J'sphere ODS series ODS-H80
ODS-M80
ODS-L80
YMC-Pack PolymerC18
120
80
80
80
2
3
5
3
5
3
5
3
5
10
5
10
5
10
3
5
3
5
10
5
10
5
4
4
4
6
10
Pages
Versatile hybrid silica based

ODS column
1.0 12.0
16 23
Great chemical durability
Excellent peak shape in every conditions
UltraHT series
Pro C18
Characteristics
2.0 8.0
12
2 m-ODS column for Ultrafast LC method

Superior separation of basic
compounds
26
Superior separation of
hydrophilic compounds
Reduced analysis time while
maintaining resolution
27
Yes
Processed with YMC Co., Ltd.s

advanced endcapping technology 32 35
Superior separation of basic compounds
12
Yes
2.0 8.0 Superior separation of

36 39
Can be used with 100% water
mobile phase
22
Yes
Highly durable ODS

1.0 10.0 Superior separation of basic compounds 40 43
and hydrophobic compounds
16
17
12
Yes
7
17
14
Yes
Yes
10
17
22
14
9
No
Yes
Yes
Yes
2.0 7.5
Currently in use worldwide
44,45
ODS with wide pore size

For separation of peptides
and proteins
44,45
Outstanding lot-to-lot reproducibility
46,47
Superior separation of
48,49
For separation utilizing residual silanol

1.0 9.0 High carbon ODS
Medium carbon ODS
2.0 7.5
Low carbon ODS
2.0 13.0 Polymer-based ODS
50,51
52,53
54,55
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Guides for selecting C18 packing materials

YMC Co., Ltd. offers twelve types of ODS columns, each with unique separation characteristics. The proper selection of packing
material is therefore a key factor in the establishment of efficient separation conditions. Selection of packing materials without sufficient
consideration of the separation characteristics may result in an expense of much time and effort in separating a target material.
Low molecular
weight compounds
High molecular
weight compounds
Chemical structures dier

slightly among solutes
YMC-Pack Pro C18 RS

Jsphere ODS-H80
Hydrophobicity diers
greatly among solutes
YMC-Pack ODS-A (Wide-Pore)

Jsphere ODS-L80
Dicult to separate
using standard ODS
Hydrosphere C18
YMC-Pack Pro C18 RS
YMC-Pack ODS-AL
Jsphere ODS-H80
Jsphere ODS-L80
YMC-Pack PolymerC18
Basic compounds
YMC-Triart C18
YMC-Pack Pro C18
Hydrosphere C18
YMC-Pack Pro C18 RS
YMC-Pack ODS-AM
YMC-Pack PolymerC18
Coordination compounds
YMC-Triart C18
YMC-Pack Pro C18
Hydrosphere C18
YMC-Pack Pro C18 RS
YMC-Pack ODS-AM
YMC-Pack PolymerC18
Sugars and glycosides
Hydrosphere C18
YMC-Pack ODS-AQ
Jsphere ODS-L80
High molecular weight peptides

and oligonucleic acids
YMC-Pack ODS-A (Wide-Pore)
Alkaline mobile phase
ODS
General organic compounds
YMC-Triart C18
YMC-Pack Pro C18
YMC-Pack ODS-A
Jsphere ODS-M80
YMC-Triart C18
YMC-Pack Pro C18 RS
Jsphere ODS-H80
YMC-Pack PolymerC18
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Versatile Hybrid Silica Based HPLC Column
YMC-Triart C18
YMC-Triart C18 is a hybrid silica based ODS column emphasizing durability, low operating pressure, and excellent performance.
YMC-Triart C18 has been developed and is manufactured using state-of-the-art technologies that define materials, granulation
and surface modification.
Rugged, long lasting, hybrid particle technology

Integration of triple artistic technologies
Benefit
Great chemical durability

Lower back pressure
Excellent resolution
U
sable over a wide pH range makes the column excellent for
method development.
Long column lifetime over wide pH range.

Low cost per analysis.
Hybrid silica base
A
bility to use on both older and newer instrumentation.
Features
R
ugged, first choice option for analysis of acids, bases and
neutral compounds.
ODS
Specifications
Base
organic/inorganic hybrid silica
Carbon %
Stationary
C18 (as USP-L1)
Bonding
polymeric type
Particle
2 m, 3 m, 5 m
Endcapping
multi-stage endcapping
Pore
120
pH range
pH 1 12
20%
YMC-Triart C18Integration of triple artistic technologies

1Versatile hybrid material
YMC-Triart C18 is a multi-layer hybrid particle consisting of two types
of layers: a silica based layer (inorganic) and a hybrid polymer based
layer (organic). This layered structure contributes to Triart's combined
physical durability and chromatographic performance.
inorganic-silane layer
Particle
synthesis by
Microreaction
Versatile
hybrid silica
material
Multi-stage
endcapping
Image structure
for hybrid-silica gel
organic-silane layer
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YMC-Triart C18Integration of triple artistic technologies

2New granulation process by microreactor technology
Homogeneous and uniform particle
Narrow pore distribution
Triart C18
4500
4000
Hybrid particle for Triart
3000
Silica particle for

conventional product
2500
2000
Hybrid particle for

competitor's product
1500
1000
500
0
Use of microreactor technology produces homogeneous and uniform

particles that results in low operating pressure and very reproducible
surface modification making for the ultimate in column-to-column and
lot-to-lot reproducibility.
50
100
150
200
Pore size ()
250
300
350
YMC-Triart C18 has a very narrow pore size distribution

that enables narrow and reproducible peak shapes for
analytes.
3Surface modification technology

Multi-stage endcapping
ODS
d Vp/d logD
3500
Endcapping methods employed for production of conventional products typically employ a single endcapping compound in one
chemical step to "neutralize" highly reactive silanols. Unfortunately, highly reactive silanols are easily hydrolyzed and contribute to
poor durability. Additionally, low reactive silanols endcap stubbornly, producing a surface modification that is often non-reproducible,
and leading to peak tailing. YMC-Triart C18 solves this problem by using an innovative, newly developed "measured multi-stage/multicompound surface modification" technology which results in the thorough endcapping of all silanols that are accessible to analytes.
Reaction image for "multi-stage endcapping"
Evaluation of silanol activity

mAU
Triart C18
60
Before
EC
STEP
STEP
40
3
20
0
0.0
Silanol group
60
40
10.0
15.0
min
mAU
Endcapping (EC) group
STEP
Q090928C
5.0
Endcapping
by traditional method
Peak tailing for basic compounds
3
1
20
2
0
0.0
Q090928E
5.0
10.0
15.0
min
Column
5 m, 1503.0 mmI.D.
Eluent
20 mM KH2PO4-K2HPO4 (pH 6.9)/acetonitrile (65/35)
Flow rate 0.425 mL/min
Temperature 40
Detection UV at 235 nm
Sample
1. Chlorpheniramine2. Dextromethorphan
3. Propyl p-hydroxybenzoate
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YMC-Triart C18
Quality ControlExcellent reproducibility

Packing Material YMC-Triart C18 exhibits excellent lot-to-lot reproducibility for all types of compounds including difficult to
chromatograph molecules that exhibit basic and coordination compound behavior.
Basic compounds
2
Lot. E
Lot. E
Lot. D
Lot. D
Lot. C
Lot. C
Lot. B
Lot. B
Lot. A
Lot. A
0.0
2.5
5.0
7.5
10.0
12.5
15.0
17.5
0.0
min
2. Dextromethorphan
12.0
14.0 min
Column
5 m, 1503.0 mmI.D.
Eluent
acetonitrile/0.1% H3PO4 (40/60)
Temperature 40
15000
2.0
10000
1.5
Tailing factor
5000
1.0
10
20
30
40
50
0.5
Low back-pressureFree from pressure problem

Column pressure and solvents
Triart C18, MeOH/W
Triart C18, ACN/W
Pro C18, MeOH/W
18
16
14
Pro C18, ACN/W
12
10
8
6
4
2
20
30
40
50
60
70
80
90
Standard ODSBest balance hydrophobicity

Comparison of separation selectivity
20
10
Column
5 m, 1504.6 mmI.D.
Eluent
acetonitrile/water (60/40)
Temperature ambient
Sample
butyl benzoate
100
Organic solvent rate (%)
Column
5 m, 1504.6 mmI.D.
Eluent
acetonitrile/water or methanol/water
Temperature 25
YMC-Triart C18 is designed for use in all conditions. Even with

higher viscosity methanol, YMC-Triart C18 can operate with
lower pressure (typically 30% less than conventional columns).
Hydrogen-bonding capacity (caffeine/benzene)
Theoretical plate number
10.0
2.5
Column number
Column pressure (MPa)
8.0
6.0
Rigid control of theoretical plate number (N) and tailing factor (Tf) are exhibited for each YMC-Triart C18 packed
column.
20000
4.0
1. Hinokitiol
2. Methyl benzoate
Column
5 m, 1503.0 mmI.D.
Eluent
Temperature 40
Packed Column
2.0
J091201X
J091201Y
1. Chlorpheniramine
3. Propyl p-hydroxybenzoate
Tailing factor
ODS
0.180
0.160
Develosil C30-UG
Hydrosphere C18
0.140
Atlantis T3
CAPCELL PAK C18 AQ
0.120
Standard ODS
CAPCELL PAK C18 MGIII

Gemini C18
The most versatile

columns as first-choice.
Pro C18
Unison UK-C18 Inertsil ODS-4

Hypersil GOLD
CAPCELL PAK C18 MGII
Gemini NX-C18
SunFire Inertsil ODS-3
XBridge
C18
XTerra MS C18
Mightysil RP-18 C18
Eternity-C18
L-column ODS
0.100
Triart C18
0.080
TSKgel ODS-100S
Cadenza CD-C18
L-column2 ODS
Develosil ODS-SR
Pro C18 RS
0.060
Eluent : MeOH/W (80/20) [amylbenzene], MeOH/W (30/70) [caffeine, benzene]
0.040
2.00
4.00
6.00
8.00
10.00
Hydrophobicity k (amylbenzene)
12.00
14.00
(S090805A)
Conventional hybrid silica based ODS columns tend to be less

hydrophobic than silica based columns. YMC-Triart C18 has a
favorable carbon balance and may be used as a "versatile firstchoice" method development column.
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Great durabilityWide usable pH range and long lifetime

Durability in high pH
Phosphate buffer (pH 11.5, 40 )
Triethylamine (pH 11.5, 40 )

Rate of theoretical plate number
120%
YMC-Triart C18
100%
80%
60%
40%
Hybrid-silica based C18
Silica based C18
20%
0%
10
20
30
40
80%
60%
40%
Silica based C18
20%
0%
50
YMC-Triart C18
100%
50
100
Time (hr)
150
200
250
ODS
120%
300
Time (hr)
Column
5 m, 1504.6 mmI.D.
Eluent
50 mM K2HPO4-K3PO4 (pH 11.5)/methanol (90/10)
Temperature 40
Sample
benzyl alcohol
Column
5 m, 1504.6 mmI.D.
Eluent
50 mM triethylamine (pH 11.5)/methanol (90/10)
Temperature 40
Sample
benzyl alcohol
Durability in high temperature
pH 6.9, 70
pH 1, 70
120%
120%
YMC-Triart C18
100%
100%
Rate of retention time
YMC-Triart C18
80%
Silica based C18

alkaline-resistant type
60%
Silica based C18
40%
Silica based C18

80%
40%
20%
0%
0%
100
200
300
400
500
600
700
60%
20%
The columns are kept in acetonitrile/water/TFA (10/90/1), 70 C,

and tested for performance every 20 hours.
0
20
40
60
80
100
120
Time (hr)
Time (hr)
Column
5 m, 502.0 mmI.D.
Eluent
Temperature 70
Sample
phenol
Test conditions Column

5 m, 502.0 mmI.D.

Eluent


Temperature 37

Sample
butyl benzoate
YMC-Triart C18 shows great chemical stability provided by newly developed hybrid silica. Even in high pH or high temperature
conditions, the lifetime of YMC-Triart C18 is more than 10 times greater than conventional ODS columns.
Reduced column bleedOperate at high sensitivity

Bleed test by LC/MS
Scan ES+
TIC
1.2E+09
Triart C18
No column
8.0E+08
Column
Eluent
4.0E+08
0.0E+00
10
12 min
5 m, 502.0 mmI.D.
A) water/formic acid (100/0.1)
B) acetonitrile/formic acid (100/0.1)
5% B (0-1 min), 5-100% B (1-5 min), 100% B (5-10 min),
100-5% B (10-10.1 min), 5% B (10.1-12.5 min)
Temperature 40
Detection ESI positive, TIC (Mass Range: 50-1000)
Bleeding of stationary phase can often contribute to high background signals when employing MS and other hyphenated detection
techniques. In the example cited above, use of a 502.0 mmI.D. YMC-Triart C18 column shows no contribution to the signal observed
by the MS positive total ion current (TIC) trace. Such performance allows for low limits of detection (high S/N ratio) for MS and other
detection techniques. The inherent hybrid particle strength is also superior to conventional silica materials for detection techniques
that are sensitive to particle degradation.
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YMC-Triart C18
Excellent resolutionWithout adsorption and tailing

Analysis of basic compounds
Ingredients in a cough/cold medication
YMC-Triart C18
5 m, 1503.0 mmI.D.
1.
Cl
CH3
2.
CH3
H3C
3
N
ODS
N
H
Q090928C
CH3
Chlorpheniramine
1
2
Silica based C18

Brand D5
5 m, 1504.6 mmI.D.
3.
O
H3C
O
Q090821C
Propyl p-hydroxybenzoate
Silica based C18

Brand N1
Column
Eluent
Flow rate
1503.0 mmI.D. or 1504.6 mmI.D.

0.425 mL/min for 3.0 mmI.D.
Temperature 40
3 m, 1503.0 mmI.D.
J091111K
1 2

Brand I8
Dextromethorphan
OH
5 m, 1504.6 mmI.D.
Q090123F
0.0
2.5
5.0
7.5
10.0
12.5
15.0
17.5
min
Analysis of coordination compounds
YMC-Triart C18's innovative surface modification

technology results in excellent peak shapes even for the
basic compounds that often exhibit tailing shapes on
conventional silica and hybrid silica based ODS columns.
Hinokitiol
CH3
1.
CH3
2.
O
CH3
YMC-Triart C18
5 m, 1503.0 mmI.D.
OH
O
Q090925G
Silica based C18

Brand N1
2
1
3 m, 1503.0 mmI.D.
J091110B

Brand I8
5 m, 1504.6 mmI.D.
0.0
2.0
4.0
Q090120A
6.0
8.0
10.0
12.0
14.0 min
Analysis of acidic compounds

YMC-Triart C18
5 m, 1503.0 mmI.D.
Q090605C
5 m, 1504.6 mmI.D.
Q090717A
1.5
2.0
1503.0 mmI.D. or 1504.6 mmI.D.

Temperature 40
YMC-Triart C18 has an extremely low level of metal

impurities, much lower than conventional products.
YMC-Triart C18 is able to provide excellent peak shape for
coordination compounds.
1. Formic acid
2. Acetic acid
3. Propionic acid
1503.0 mmI.D. or 1504.6 mmI.D.

Temperature 37

Brand G4
1.0
Column
Eluent
Flow rate
Column
Eluent
Flow rate
0.5
Methyl benzoate
Organic acid
0.0
Hinokitiol
2.5
3.0
3.5
4.0
4.5
5.0
5.5
6.0
6.5
7.0
7.5 min
YMC-Triart C18 is synthesized utilizing methodology

borrowed from microreactor technology. This synthesis
technique allows for a reduction of impurities that contribute
to peak tailing during the analysis of some types of acidic
compounds.
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Excellent versatilityBuffer independency

Comparison of clemastine analysis with conventional columns
10 mM phosphate buffer (pH 6.7)/organic solvent

methanol
10 mM CH3COONH4/organic solvent
methanol
acetonitrile
acetonitrile
YMC-Triart C18
502.0 mmI.D.
Tf 0.97
Tf 1.11
Tf 1.21
502.1 mmI.D.
Tf 1.56
F090727H07
Silica based C18

Brand D5
Silica based C18

Brand I6
Tf 1.03
Hybrid-silica
based C18
Brand I8
Tf 1.14
502.1 mmI.D.
10.0
H3C
N
11.0
12.0
Tf 1.78
F090731G07
F090731F07
min
8.0
9.0
10.0
min
6.0
7.0
8.0
min
Column
Eluent
5 m, 502.0 mmI.D. or 502.1 mmI.D.

A) 10 mM KH2PO4-K2HPO4 (pH 6.7) or 10 mM CH3COONH4 (pH 6.7)
B) methanol or acetonitrile
5-90% B (0-10 min), 90% B (10-15 min)
Temperature 25
CH3
8.0
F090722G07
Tf 1.22
F090731I07
7.0
Tf 1.88
F090722F07
Tf 1.88
6.0
F090714G07
Tf 1.13
F090722I07
min
Tf 2.21
F090714F07
Tf 3.87
F090731H07
502.1 mmI.D.
F090803G07
Tf 1.05
F090717I07
F090722H07
Tf 1.43
F090727F07
Tf 2.83
F090717H07
F090817G07
Tf 1.13
F090727I07
Tf 0.93
502.0 mmI.D.
Tf 1.16
F090818F07
ODS
Silica based C18

Brand C1
Tf 1.03
F090818I07
F090818H07
Cl
Clemastine
Clemastine is a well known basic compound which can easily tail on conventional ODS columns. YMC-Triart C18 can separate
clemastine sharply using many buffer/solvent combinations.
Improvement of loadabilityMinimizing strong solvent/sample loading effects

Influence on peak shapes by injection volume
1

2
solvent for sample
injection volume
acetonitrile
1.0 L
1.5 L
2.0 L
3.0 L
YMC-Triart C18
Column
Eluent
5 m, 502.0 mmI.D. or 2.1 mmI.D.

A) water/formic acid (100/0.1)
B) acetonitrile/formic acid (100/0.1)
5% B0-0.5 min
, 5-100% B (0.5-2.5 min)
Temperature 40
Silica based C18

1.
Peak anomaly at 1.5 L
2.
OCH2CHCH2NHCH(CH3)2
OH
H3CO
H3CO
CN
CH3
C (CH2)3NCH2CH2
OCH3
OCH3
CH(CH3)2
1.5
2.0
2.5
min
Propranolol (50 g/mL)
Verapamil (50 g/mL)
YMC-Triart C18 can tolerate larger injection volumes of samples containing strong solvents (e.g., acetonitrile) while allowing for
better peak shape than traditional columns. This can be important for difficult to solubilize samples that need higher concentrations of
organic solvent for solubilization prior to analysis.
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YMC-Triart C18
Fast analysis of paraquat and diquat

YMC-Triart C18 5 m, 1502.0 mmI.D.

mAU
10%B, 0.2 mL/min, 5.7 MPa
25
20
ODS
mAU
20
Eluent optimization
Insufficient
separation
15
10
R091126J
Baseline separation,
But long analysis time
15
10
5%B, 0.2 mL/min, 5.6 MPa
25
10
12
14 min
R091127C
10
15
20
25 min
Downsizing
1.
2.
N+
N+
mAU
2Br
N+ CH3
2Cl
H3C N+
25
20
Paraquat
Diquat
5%B, 0.4 mL/min,

12.2 MPa
80% reduction in run time
15
10
Eluent
A) water/HFBA* (100/0.1)

B) acetonitrile/HFBA* (100/0.1)
Temperature 37
Injection
1 L (0.1 mg/mL)
*heptafluorobutyric acid
5
0
R091201B
Application
min
Q091110E
Anthocyanin
1. Delphinidin-3-O-galactoside
2. Delphinidin-3-O-glucoside
3. Cyanidin-3-O-galactoside
4. Delphinidin-3-O-arabinoside
5. Cyanidin-3-O-glucoside
6. Petunidin-3-O-galactoside
7. Cyanidin-3-O-arabinoside
8. Petunidin-3-O-glucoside
9. Peonidin-3-O-galactoside
10. Petunidin-3-O-arabinoside
11. Delphinidin
12. Peonidin-3-O-glucoside
13. Malvidin-3-O-galactoside
14. Peonidin-3-O-arabinoside
15. Malvidin-3-O-glucoside
16. Malvidin-3-O-arabinoside
17. Cyanidin
18. Petunidin
19. Peonidin
20. Malvidin
mAU
100
80
4
5
60
3
8
7
40
15
6
16
17
12
20
10
11 13
18 20
19
14
0
0
10
15
20
25
30
35
40
min
Column
YMC-Triart C18 (5 m, 120 )

2504.6 mmI.D.
Eluent
A) water/formic acid (90/10)
B) acetonitrile/methanol/water/formic acid
(22.5/22.5/40/10)
20-28% B (0-30 min), 28-70% B (30-40 min),
100% B (40-45 min)
Temperature 25
Detection UV/VIS at 535 nm
Sample
commercial bilberry powder (1.25 mg/mL)
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Application
R091009C
Peptide
mAU
1. Oxytocin
2. Met-Enkephalin
3. Leu-Enkephalin
4. Neurotensin
5. -Endorphin
6. -Endorphin
100
1
75
50
Column
5
25
10
15
25 min
20

1502.0 mmI.D.
Eluent
A) water/TFA (100/0.1)
B) acetonitrile/TFA (100/0.1)
20-45% B (0-25 min)
Temperature 37
Application
S100121A
LC/MS analysis for Triazolam and Etizolam

Intens .
x104
6
High resolution extracted chromatograms
1.
+
forM+H
H3C
N
N
Cl
2.
N
N
Mixture 100 ppb
H3C
S
H3C
N
Cl
Cl
Triazolam (MW 343.22)
Etizolam (MW 342.85)
Column
1
0
0
10
12
14
Time [min]
100ng_mL_RB4_01_2291.d: EIC 343.0510.01 +All MS

100ng_mL_RB4_01_2291.d: EIC 343.0780.01 +All MS
Courtesy of J.Watanabe, Bruker Daltonics K. K.

502.0 mmI.D.
Eluent
A) 10 mM HCOOH, B) acetonitrile
25-50% B (0-10 min), 25% B (10-15 min)
Temperature 40
Detection ESI, positive mode (Bruker Daltonics micrOTOF)
Injection
5 L
ODS
(MW 1,007)
(MW 574)
(MW 556)
(MW 1,673)
(MW 1,859)
(MW 3,465)
Ordering information
Analytical columns
Particle size
m
S-3
S-5
Semi-preparative columns
Pore size Column size
inner diameterlengthmm
120
120
Please inquire for 2 m columns.
2.0 20
2.0 30
2.0 50
2.0 75
2.0100
2.0150
3.0 50
3.0100
3.0150
4.6 35
4.6 50
4.6 75
4.6100
4.6150
2.0 20
2.0 30
2.0 50
2.0 75
2.0100
2.0150
3.0 50
3.0 75
3.0100
3.0150
4.6 50
4.6 75
4.6100
4.6150
4.6250
Product number
TA12S03-0202WT
TA12S03-0302WT
TA12S03-0502WT
TA12S03-L502WT
TA12S03-1002WT
TA12S03-1502WT
TA12S03-0503WT
TA12S03-1003WT
TA12S03-1503WT
TA12S03-H546WT
TA12S03-0546WT
TA12S03-L546WT
TA12S03-1046WT
TA12S03-1546WT
TA12S05-0202WT
TA12S05-0302WT
TA12S05-0502WT
TA12S05-L502WT
TA12S05-1002WT
TA12S05-1502WT
TA12S05-0503WT
TA12S05-L503WT
TA12S05-1003WT
TA12S05-1503WT
TA12S05-0546WT
TA12S05-L546WT
TA12S05-1046WT
TA12S05-1546WT
TA12S05-2546WT
Particle size
m
S-5
120
10150
10250
20100
20150
20250
Product number
TA12S05-1510WT
TA12S05-2510WT
TA12S05-1020WT
TA12S05-1520WT
TA12S05-2520WT
Semi-preparative columns YMC-Actus Triart C18

TA12S05-0520WX
20 50
S-5
120
20100
30 50
30 75
30100
TA12S05-1020WX
TA12S05-0530WX
TA12S05-L530WX
TA12S05-1030WX
See P138-141 for details of YMC-Actus series.
Guard cartridge columns (with inner diameter 2.0 mm: 2/pack, 4.0 mm: 3/pack)
TA12S03-0102CC
2.010
S-3
120
S-5
120
4.023
2.010
4.023
TA12S03-G304CC
TA12S05-0102CC
TA12S05-G304CC
A cartridge holder will need to be purchased separately before using this product for the first time.
Cartridge holder
Semi-micro guard cartridge holder
(inner diameter: 1.0, 1.5, or 2.0 mm)
Cartridge holder
(inner diameter: 4.0 mm)
Guard columns
S-5
120
1030
2050
XPCHSMW
XPCHW
TA12S05-0310WTG
TA12S05-0520WTG
23
01.indd 23
11.1.28 10:39:40 AM
Ultra-Fast LC column
YMC-UltraHT
ODS
YMC-UltraHT series is a C18 bonded-phase based on
standard ODS, and Hydrosphere C18 for hydrophilic
highly efficient 2 m spherical silica particles. YMC-
compounds. Both of them show the same selectivity
UltraHT series columns are specifically designed for
and superior peak shapes as 3 m and 5 m Pro series,
high-speed and high throughput analysis in various fields
allowing for an easy method transfer from conventional
such as pharmaceuticals, foods, and environmentals.
HPLC to ultra-fast HPLC without changing elution
YMC-UltraHT series has two products. Pro C18 as our
conditions.
2 m ODS column ideal for Ultra-Fast LC

Features
Superior column performance at higher flow rate and higher pressure
Reduced analysis time while maintaining excellent resolution
Excellent resolution with back pressure less than that of sub-2 m
Applicable to both conventional HPLC and specific ultra pressure LC system
Same selectivity and superior peak shapes as 3 m and 5 m Pro series
S
imple method transfer from conventional HPLC on Pro series; no need to
change elution conditions
Characteristics of 2 m packing material

Van Deemter curves
Column pressure
0.020
80
Optimum ow rate
for 5 m
Optimum ow rate for 2 m

(0.3-0.8 mL/min for 2 mmI.D.)
0.015
YMC 3 m
0.010
Brand L1 1.8 m
YMC 2 m
0.005
Optimum ow rate for 2 m
70
YMC 5 m
Column pressure (MPa)
HETP (mm/plates)
(0.2 mL/min for 2 mmI.D.)
Brand I7 1.7 m
Brand I7 1.7 m
(0.3-0.8 mL/min for 2 mmI.D.)
Brand L1 1.8 m
60
50
40
YMC 2 m
applicable
pressure range
for conventional LC
30
YMC 3 m
20
YMC 5 m
10
0.000
10
11
Interstitial linear velocity (mm/sec)
10
11
Interstitial linear velocity (mm/sec)
YMC-UltraHT series shows nearly the same efficiency as sub-2

m columns for ultra fast LC system and reduces the column
pressure significantly.
Column
: 502.0 or 2.1 mmI.D.
Eluent
: acetonitrile/water (60/40)
Temperature : 25
Sample
: Butyl benzoate
YMC-UltraHT series
Pro C18
Particle Size (m)
Pore Size ()
Hydrosphere C18
120
120
Specific Surface area (m2/g)
330
330
Carbon Content
16%
12%
Monomeric
Monomeric
Bonding Type
Available pH Range
Characteristics
2-8
2-8
Standard type
For polar analytes
24
01.indd 24
11.1.28 10:39:43 AM
Ultra Fast analysis for flavonoids

conventional LC methodYMC-Pack
C18
1504.6 mmI.D. 5 m
tR(7) 24.3 min

N(7) 77,000 /m
1.0 mL/min
5 L injection
8.5 MPa
Ultra-fast LC methodYMC-UltraHT
C18
502.0 mmI.D. 2 m
Flavonoids
90% Decrease in analysis time

90% Increase in efficiency
1. Myricetin
2. Quercetin
3. Apigenin
4. Kaempferol
5. Baicalein
6. Chrysin
7. Acacetin
tR(7) 2.7 min

N(7) 144,000 /m
0.6 mL/min
1 L injection
30 MPa
Eluent
: acetonitrile/water/formic acid

(35/65/0.1)
Temperature : 40
Detection : UV at 260 nm
ODS
Triplear
line city
Velo
This example shows the method transfer from conventional HPLC method to Ultra-fast LC method using a YMC-UltraHT column. Since
the column efficiency is not decreased even when the flow rate is 3 times as fast as the conventional condition, it can be shorten
analysis time by 90% without losing the separation characteristics.
Applicable for conventional LC system

503 mmI.D.
Conventional semi-micro LC system2.5 L, cell length 5 mm

mAU
80
Flow rate : 0.8 mL/min

Injection : 2 L
60
40
20
0
502 mmI.D.
Conventional semi-micro LC system2.5 L, cell length 5 mm

80
N(1) = 6300
N(2) = 6300
N(3) = 7500
N(4) = 7800
Rs(1, 2) = 2.21
mAU
1
100
50
3 min
N(1) = 3500
N(2) = 3800
N(3) = 6100
N(4) = 6800
Rs(1, 2) = 1.61

Injection : 1 L
mAU
150
3 min
YMC-UltraHT series can be used for fast analysis with conventional

LC system, as its pressure is lower than commercial sub-2 m columns.
Considering the extra column band broadening, the column of 3.0 mml.D. is
more applicable for conventional LC system than the column of 2.0 mml.D..
12
100
50
0
Conventional LC system8 L, cell length 10 mm
(N070606E)
N(1) = 3700
N(2) = 4100
N(3) = 6100
N(4) = 6900
Rs(1, 2) = 1.61
(N070608E)

Injection : 2 L
20
3 min
Conventional LC system8 L, cell length 10 mm
40
0
1
150
60
(N070606B)

Injection : 1 L
mAU
N(1) = 1400
N(2) = 1500
N(3) = 3600
4
N(4) = 4500
Rs(1, 2) = 0.90
(N070608B)
3 min
Cephalosporin antibiotics
1. Cephalexin
2. Cefaclor
3. Cephaloglycin
4. Cephaloridine
Column
YMC-UltraHT Pro C18
Eluent
acetonitrile/20 mM KH2PO4

(10/90)
Temperature 37
25
01.indd 25
11.1.28 10:39:45 AM
Analytical columns
YMC-UltraHT Pro C18

Excellent resolution with back pressure less than 60% of commercial sub-2 m
Same selectivity and superior peak shapes as 3 m and 5 m Pro C18
Pore size120
Carbon content16
Usable pH range2.0 8.0
Simple method transfer from conventional HPLC
First choice ODS column for Ultra-fast LC

YMC-UltraHT Pro C18 is a C18 bonded-phase based
on highly efficient 2 m spherical silica particles. YMCUltraHT Pro C18 is designed for high-speed and high
throughput analysis, advanced endcapping technology
ODS
makes YMC-UltraHT Pro C18 highly appropriate for

analysis of basic compounds. The physical properties and
separation characteristics are strictly controlled, so it is
highly suitable as the first choice column for ultra fast LC.
Excellent peak shapes

mV
60
YMC-UltraHT
C18
502.0 mmI.D 2 m
1
40
N(5) 106,000/m
Tf(5) 1.07
30 MPa
20
mV
60
sub-2 m ODS
2
20
N(5) 89,000/m
Tf(5) 3.99
49 MPa
40
mV
60
40
0.4
0.8
1.2
1.6
2.0
[min]
N(5) 78,000/m
Tf(5) 2.35
37 MPa
1.2
1.6
20
0
0.0
sub-2 m ODS
0.0
0.4
0.8
1.2
1.6
Alkaloids
1. Scopolamine
2. Atropine
3. Cinchonine
4. Quinine
5. Dihydroquinine
YMC-UltraHT Pro C18 exhibits greater peak shapes of

alkaloids, which are highly basic compounds compared to
commercial silica based sub-2 m columns.
2.0
[min]
0.0
0.4
0.8
2.0
[min]
Column
: 502.0 or 2.1 mmI.D.
Eluent
:2
0 mM CH3COOH-CH3COONH4 (pH 4.9)/

acetonitrile (80/20)
Flow rate
: 0.6 mL/min
Temperature : 40
Peptide mapping
mV
YMC-Pack
C18
1502.0 mmI.D. 5 m
0%B (0-5 min)

0-100%B (5-40 min)
100%B (40-45 min)
80
40
(06082204.D)
15
same ow rate, column length 1/3x, gradient time 1/3x
mV
YMC-UltraHT
C18
502.0 mmI.D. 2 m
200
45
30
100
min
0%B (0-1.65 min)

0-100%B (1.65-13.35 min)
100%B (13.35-15 min)
*undigested
Cytochrome c
N060811I
10
15
min
Analysis of a tryptic digest of cytochrome C is

shown below. A 50mm length UltraHT Pro C18
column of 2 m particle can be shorten the
analysis time by one-thirds compared to a 150
mm column packed with 5 m Pro C18.
Eluent
A) acetonitrile/water/TFA (10/90/0.1)

B) acetonitrile/water/TFA (35/65/0.1)
Temperature 37
Sample
tryptic degest of Cytochrome c
Analytical columns
Particle size
m
S-2
120
2.0 30
2.0 50
2.0 75
2.0100
3.0 50
3.0 75
3.0100
Product number
AS12S02-0302WT
AS12S02-0502WT
AS12S02-L502WT
AS12S02-1002WT
AS12S02-0503WT
AS12S02-L503WT
AS12S02-1003WT
26
01.indd 26
11.1.28 10:40:11 AM
Analytical columns
YMC-UltraHT Hydrosphere C18

Can be used with 100% aqueous as mobile phase
Reduced analysis time while maintaining excellent resolution
Pore size120
Carbon content12
The best column for hydrophilic compounds

YMC-UltraHT Hydrosphere C18 is a C18 bonded phase
It is designed to maintain hydrohilicity on the packing
based on highly efficient 2 m spherical silica particles.
surface for superior separation of hydrophilic compounds.
Hydrosphere C18504.6 mmI.D. 3 m
Rs(10,11)=2.38
2
4
11
12
10
7 8
Examples illustrated shows the

optimization process of isoflavones
separation by 2 m YMC-UltraHT
Hydrosphere C18. It has the same
selectivity as 3 m Hydrosphere C18,
so there is no need to change the
condition at downsizing. Maintaining
resolution, the analysis time can
be reduced by using 2 m packing
material.
12.5-30% B (0-10 min)

1.5 mL/min
3 L injection
(N060509E)
10 min
downsizing the particle size and column inner diameter
ODS
Ultrafast analyses of soy isoflavones
YMC-UltraHT Hydrosphere C18 2m502.0 mmI.D.

YMC-UltraHT Hydrosphere C18502.0 mmI.D. 2 m
Rs(10,11)=2.91
Rs(10,11)=2.91
Rs(7,8)=2.46
3
3
1
1 2
2
9
9
5
4 5
4
6
6
12
12
10
10 11
11
7
7 8
8
12.5-30% B (0-10 min)

0.3 mL/min
12.5-30% ACN (0-10 min)
0.6 L injection
0.3 mL/min
MPa
(N070130I)
(N070130I)
0
0
2
2
4
4
6
6
Soy isoflavones
10
10 min
min
8
8
triple ow rate and optimizing the gradient condition
YMC-UltraHT Hydrosphere C18 2m502.0 mmI.D.

YMC-UltraHT Hydrosphere C18502.0 mmI.D. 2 m
3
3
1
1 2
2
Rs(7,8)=1.75
5
4
5
4
6
6
7 8
7 8
Rs(10,11)=2.64
Rs(10,11)=2.64
9
9
10
10
12
12
11
11
12.5-30% B (0-3.3 min)

0.9
mL/minACN (0-3.3 min)
12.5-30%
0.6
L injection
0.9 mL/min
1. Daidzin
2. Glycitin
3. Genistin
4. 6"-O-Malonyldaidzin
5. 6"-O-Malonylglycitin
6. 6"-O-Acetyldaidzin
7. 6"-O-Acetylglycitin
8. 6"-O-Malonylgenistin
9. Daidzein
10. Glycitein
11. 6"-O-Acetylgenistin
12. Genistein
MPa
(N070130L)
0
0
(N070130L)
1
1
2
2
3
3
3.3 min
3.3 min
Eluent
A) water/acetic acid (100/3)

B) acetonitrile/acetic acid (100/3)
Temperature 35
Analytical columns
Particle size
m
S-2
120
2.0 30
2.0 50
2.0 75
2.0100
3.0 50
3.0 75
3.0100
Product number
HS12S02-0302WT
HS12S02-0502WT
HS12S02-L502WT
HS12S02-1002WT
HS12S02-0503WT
HS12S02-L503WT
HS12S02-1003WT
27
01.indd 27
11.1.28 10:40:30 AM
High performance reversed-phase column

Pro series high performance reversed-phase columns
We provide products with excellent performance and
feature advanced endcapping technology and quality
stable quality all over the world to fulfill the needs of the
assurance which is ready for validation. The Pro series
pharmaceutical industry, in which production, as well as
includes three kinds of ODS, each with unique separation
research and development, are increasingly globalized.
characteristics.
ODS
Providing products with stable quality all over the world

Feature
Silica

gel base, low in metal impurities
Processed

with advanced endcapping
technology
Superior

separation of basic compounds
U
seful for LC/MS
E
xcellent durability
E
xcellent reproducibility
Two

types of test reports are included
with each column
Standard ODS column

with high versatility
Low carbon ODS column,

useful for separation of
High carbon ODS column,

useful for separation of highly
hydrophobic compounds
YMC-Pack Pro C18
Hydrosphere C18
YMC-Pack Pro C18 RS
Hydrosphere C18 is an ODS column
YMC-Pack
performance monomeric type ODS
YMC-Pack
C18 is a high
designed to maintain maximum
carbon "polymeric type" ODS bonding,
C18 RS is a high
column providing standard
hydrophilicity without activating the
characterized by high resolution and
hydrophobicity, high resolution, high
silica surface. It can be used with
high durability. It is applicable to a
durability and excellent reproducibility.
100% water eluent, a problematic
wide range of compounds. The
It is highly versatile and suitable as the
eluent for conventional ODS columns.
separation of compounds with relatively
rst choice column in almost all elds,
Hydrosphere C18 is useful for
high hydrophobicity and those that dier
including pharmaceutical products,
separating hydrophilic compounds.
only slightly in hydrophobicity, such as
agricultural chemicals, foods, and
structural isomers, is outstanding. It
natural products.
also has excellent resistance to acid

and alkali, making it useful under
demanding separation conditions.
Types of Pro series ODS

Particle Size (m)
Pore Size ()
Pro C18
Hydrosphere C18
Pro C18 RS
2 , 3 , 5 , 10
2,3,5
3,5
120
120
80
Specific Surface area (m /g)
330
330
510
Carbon Content
16%
12%
22%
monomeric
monomeric
polymeric
2-8
2-8
1 - 10
Standard type
Separation for hydrophilic compounds
High durability
Bonding type
Available pH Range
Characteristics
28
01.indd 28
11.1.28 10:40:32 AM
Three kinds of ODS, each with different separation characteristics

The three kinds of ODS columns in the Pro series
each have unique carbon content and separation
characteristics, including hydrophobicity, plane
recognition ability, and hydrogen-bonding capacity.
Selection according to the analyte and mobile phase
of Pro C18 with standard hydrophobicity, Pro C18 RS
with high hydrophobicity, or Hydrosphere C18 with low
hydrophobicity, facilitates method development.
Hydrophobicity and plane recognition ability of Pro series ODS
C18
Hydrosphere C18
3
C18 RS
1
5
2
2
YMC-Pack
3
4
5
4
J020805B
1. Uracil
2. n-Butylbenzene
3. o-Terphenyl
4. n-Amylbenzene
5. Triphenylene
10
15
20 min
J020805D
10
15
20 min
J020805E
10
15
20
25 min
ODS
YMC-Pack
Column size 1504.6 mmI.D.

Eluent
methanol/water (80/20)
Temperature 37
Hydrogen-bonding capacity (caffeine/benzene)
Comparison of hydrophobicity and hydrogen-bonding capacity of various columns

0.180
Hydrophobicity: low
Hydrogen-bonding capacity: high
0.160
Develosil C30-UG
Hydrosphere C18
0.140
Atlantis T3
CAPCELL PAK C18 AQ
0.120
Hydrophobicity: intermediate
Hydrogen-bonding capacity: middle
CAPCELL PAK C18 MGIII

Gemini C18
Pro C18
Unison UK-C18 Inertsil ODS-4

Hypersil GOLD
CAPCELL PAK C18 MGII
Gemini NX-C18
SunFire Inertsil ODS-3
XBridge
C18
XTerra MS C18
Mightysil RP-18 C18
Eternity-C18
L-column ODS
0.100
0.080
TSKgel ODS-100S
Cadenza CD-C18
L-column2 ODS
Hydrophobicity: high
Hydrogen-bonding capacity: low
Develosil ODS-SR
Pro C18 RS
0.060
Eluent : MeOH/W (80/20) [amylbenzene], MeOH/W (30/70) [caffeine, benzene]
0.040
2.00
4.00
6.00
8.00
10.00
Hydrophobicity k (amylbenzene)
12.00
14.00
(S090805A)
29
01.indd 29
11.1.28 10:40:35 AM
High performance reversed-phase column
Comparison of separation behavior on the three kinds of ODS columns in the Pro series
Parabenzoates
C18
ODS
3 types of ODS are available to

separate the para-benzoates
shown in the left, but the best
separation between peaks 5 and
6 is achieved on Pro C18 RS.
1
1
1.
OH
2
2
COOCH3
Methyl p-hydroxybenzoate
2.
OH
34
3 4
COOCH2CH3
Ethyl p-hydroxybenzoate
34
3.
CH3
OH
COOCH
CH3
56
Isopropyl p-hydroxybenzoate
5 6
4.
OH
C18 RS
Hydrosphere C18
COO(CH2)2CH3
Propyl p-hydroxybenzoate
5.
OH
20 min
10
J020827D
J020827C
10
20 min
J020827E
10
20
30 min
CH3
COOCH2CH
CH3
Isobutyl p-hydroxybenzoate
6.
OH
Column
1504.6 mmI.D.
Eluent
Temperature 30
COO(CH2)3CH3
Butyl p-hydroxybenzoate
Flavonoid glycosides
2
1
C18
J020828A
HO
OH
J020828F
2
3
4
C18 RS
J020828C
OH
Hydrosphere C18
HO
10
15
min
1. Rutin
2. Isoquercitrin
3. Quercitrin
4. Quercetin
OR
O
R
R
R
R
= rutinose
= glucose
= rhamnose
=H
Hydrogen-bonding
interactions significantly
contribute to the retention
of hydrophilic compounds,
including glycosides.
Therefore, the retention is
longer in the Hydrosphere
C18, which has strong
hydrogen-bonding capacity,
than in Pro C18 RS, which
has highly hydrophobic
packing materials. To
separate such hydrophilic
compounds, the Hydrosphere
C18 is suitable for
precise separation of
multicomponent samples,
while Pro C18 RS and
Pro C18 are suitable for
rapid separation of simple
components.
Column
1504.6 mmI.D.
Eluent
acetonitrile/10 mM H3PO4 (25/75)
Temperature 40
30
01.indd 30
11.1.28 10:40:39 AM
Terphenyl isomers
C18
C18 RS
Hydrosphere C18
Brand C1
2,3
1.
1 3
1
o-Terphenyl
1
1
2.
m-Terphenyl
3.
0
J020719J
10
20 min
J020722A
10
20 min
J020719E
10
20 min
J020719K
10
ODS
Since Pro C18 RS has

excellent hydrophobic
selectivity and shape
selectivity, it works
well in the separation
of structural isomers
that are not easily
separated by other
ODS.
20 min
p-Terphenyl
Column
1504.6 mmI.D.
Eluent
methanol/water

(85/15)
Temperature 37
Saikosaponins
Peaks 2 and 3, which are not easily separated by other
ODS, can be completely separated by Hydrosphere C18. It
is thought that hydrogen-bonding interaction of hydrophilic
functional groups in sugar chain and terpene skeleton as
well as hydrophobic interaction has great contribution to the
separation of saikosaponins.
Hydrosphere C18 is the most appropriate in the separation of
this kind of class of compound.
3
2
C18
F030222B
Hydrosphere C18
23
1.
3.
CH2OH
OH
OH
F030222A
1
2,3
glucose 6 glucose
fucose
rhamnose
C18 RS
Saikosaponin c
2.
H
OH
OH
O
2,3
fucose
4
10
20
30
CH2OH
glucose
Saikosaponin a
F030224E
4.
Brand B2
CH2OH
glucose
Saikosaponin b2
F030222C
40 min
fucose
CH2OH
glucose
Saikosaponin d
Column
1504.6 mmI.D.
Eluent
Temperature 37
31
01.indd 31
11.1.28 10:40:44 AM
Analytical columns
YMC-Pack Pro C18

S
uperior separation of basic
compounds
P
rocessed with advanced
Excellent reproducibility
Utilizes highly pure silica gel base
T
wo types of test reports are included
endcapping technology
Pore size120
Carbon content16
with each column
Perfectly endcapped ODS

YMC-Pack Pro C18 includes more advanced endcapping technology for
strictly controlled processing of residual silanol groups that are likely
to affect quality. Pro C18 is highly appropriate for basic compounds,
including pharmaceutical products and agricultural chemicals.
ODS
Two test reports are included with each product; a Column Test Report,
indicating the column performance, and a Packing Material Test
Report, detailing strictly controlled physical properties and separation
characteristics of the packing material.
Useful for separation of basic compounds

Tricyclic antidepressants
1.
23
1
2.
Advanced endcapping technology

results in good peak shapes even
for basic compounds that often
elute with poor tailing shapes on
competitive columns.
HCI
N
CH2CH2CH2NHCH3
Desipramine hydrochloride
Acenaphthene(I.S.)
3.
4.
HCI
HCI
N
CH2CH2CH2N
CH3
CHCH2CH2N
CH3
Imipramine hydrochloride
10
CH3
CH3
Amitriptyline hydrochloride
20 min
A961015G
Column
YMC-Pack Pro C18 (5 m, 120 )

1504.6 mmI.D.
Eluent
20 mM KH2PO4-K2HPO4 (pH6.9)/

methanol (25/75)
Temperature 37
Basic peptides
C18
1. Bradykinin
2. Dynorphin A (Human,1-13)
3. Eledoisin related peptide
4. Neurotensin
5. Bombesin
6. Substance P
ODS-A
4
6
2
3
2
3
10
15
20
min
A990123B
10
15
20
min
A990121A
Neutral peptides
C18
2
3
ODS-A
5
4
2
6
YMC-Pack Pro C18 is processed

using advanced endcapping
technology and produces a
better chromatogram than
that obtained using ODS-A for
separating basic peptides.
Similar chromatograms have
been obtained with YMCPack Pro C18 and ODS-A for
separation of neutral peptides.
Column
YMC-Pack Pro C18 and ODS-A (5 m, 120 )

754.6 mmI.D.
Eluent
A) water/TFA (100/0.1)


20-40 B (0-20 min,linear)
Temperature 37
5
4
A990121B
10
15
20 min
A990119B
10
15
20 min
1. Oxytocin
2. Met-Enkephalin
3. Leu-Enkephalin
4. Angiotensin l
5. -Mating factor
6. Insulin
32
01.indd 32
11.1.28 10:40:48 AM
Excellent lot-to-lot reproducibility

batch-to-batch
Test A
Test B
1. Caffeine
2. Pyridine
3. Phenol
Lot C
Lot C
Lot B
Lot B
Lot A
Lot A
10
15
A three-lot comparison is shown on the left.

Excellent reproducibility is achieved in
separating hydrophilic, basic and acidic
compounds, as well as hydrophobic
compounds.
ODS
1. Uracil
2. 4-n-Butoxybenzoic acid
3. Ethylbenzene
4. n-Propylbenzene
min
min
column-to-column
1504.6 mml.D. theoretical plate number
Advanced packing technology results in high

column efficiency. All packed columns are
inspected and controlled according to the
strictest quality control criteria for column
performance ever, including theoretical plate
number testing.
24000
21000
18000
15000
12000
9000
6000
3000
20
40
60
80
100
Column No.
Two types of test reports

Packing Material Test Report
Column Test Report

Each YMC-Pack Pro C18
includes both, a Column Test
Report and a Packing Material
Test Report, to certify lotto-lot and column-to-column
reproducibility.
This report describes basic physical properties of silica
The Column Test Report shows the theoretical plate number
gel, including particle size, pore size and metal content,
and asymmetry factor to indicate column performance. For
carbon loading after modification, and the specifications
convenience in deterioration evaluation, this report includes all
and results of separation characteristics tests.
analytical conditions including sample concentration as well.
33
01.indd 33
11.1.28 10:40:51 AM
Analytical columns
YMC-Pack Pro C18
Application
3
4 5
C18
5
3
Brand I4
1
4, 5
Brand L1
1
2
2
6
0
10
F040119B
15
20 min
10
F030130C
15
20 min
F040130E
10
15
20 min
nasal drops (OTC)

1
2
CHCOOH
CHCOOH
CH3 O
NHCCH2 N(CH2 CH3 )2
CH3
4
H
Maleic acid
Lidocaine
6
OH
COOCH3
CH3
CH3
CH3
Chlorpheniramine
CH3
CH3 C CH2 C
CH3
CH3
Methyl p-hydroxybenzoate
Cl
Naphazoline
CH3
N
CHCH2CH2N
CH2
ODS
(OCH2CH2)2 N CH2
CH3
Cl-
Column
1504.6 mmI.D.
Eluent
A) 20 mM KH2PO4-H3PO4 (pH 2.5)

B) methanol

20-90% B (0-15 min), 90% B (15-20 min)
Temperature 37
Benzethonium chloride
Application
N050620A
catechins
1
1. (-)-Epigallocatechin
2. (+)-Catechin
3. (-)-Epicatechin
4. (-)-Epigallocatechin gallate
5. (-)-Epicatechin gallate
Column
: YMC-Pack Pro C18 (5 m, 120 )

1504.6 mmI.D.
Eluent
: acetonitrile/ethyl acetate/water/TFA

(9/3/88/0.1)
Flow rate
: 1.0 mL/min
Temperature : 37
10
20
min
Application
A970526C
antioxidants
5
1. n-Propyl gallate(PG)
2. 2,4,5-Trihydroxybutyrophenone (THBP)
3. tert-Butylhydroquinone(TBHQ)
4. Nordihydroguaiaretic acid (NDGA)
5. 4-Hydroxymethyl-2,6-di-tert-butylphenol (HMBP)
6. n-Octyl gallate(OG)
7. n-Dodecyl gallate(DG)
4
6
1
Column
YMC-Pack Pro C18 (5 m, 120 )

754.6 mm I.D.
Eluent
A) acetonitrile/methanol/5% acetic acid (20/20/60)

B) acetonitrile/methanol/5% acetic acid (45/45/10)

0-100% B (0-5 min), 100% B (5-15 min)
Temperature 30
10
15
min
34
01.indd 34
11.1.28 10:40:55 AM
Analytical columns
Particle size
m
S-3
S-10
120
120
120
1.0 35
1.0 50
1.0100
1.5 35
1.5 50
1.5100
2.0 35
2.0 50
2.0 75
2.0100
2.0150
3.0 50
3.0100
3.0150
4.6 35
4.6 50
4.6 75
4.6100
4.6150
6.0 50
6.0 75
6.0100
1.0150
1.0250
1.5150
1.5250
2.0 35
2.0 50
2.0 75
2.0100
2.0150
2.0250
3.0 50
3.0 75
3.0100
3.0150
3.0250
4.6 50
4.6 75
4.6100
4.6150
4.6250
6.0150
6.0250
4.6150
4.6250
Product number
AS12S03-H501WT
AS12S03-0501WT
AS12S03-1001WT
AS12S03-H5P5WT
AS12S03-05P5WT
AS12S03-10P5WT
AS12S03-H502WT
AS12S03-0502WT
AS12S03-L502WT
AS12S03-1002WT
AS12S03-1502WT
AS12S03-0503WT
AS12S03-1003WT
AS12S03-1503WT
AS12S03-H546WT
AS12S03-0546WT
AS12S03-L546WT
AS12S03-1046WT
AS12S03-1546WT
AS12S03-0506WT
AS12S03-L506WT
AS12S03-1006WT
AS12S05-1501WT
AS12S05-2501WT
AS12S05-15P5WT
AS12S05-25P5WT
AS12S05-H502WT
AS12S05-0502WT
AS12S05-L502WT
AS12S05-1002WT
AS12S05-1502WT
AS12S05-2502WT
AS12S05-0503WT
AS12S05-L503WT
AS12S05-1003WT
AS12S05-1503WT
AS12S05-2503WT
AS12S05-0546WT
AS12S05-L546WT
AS12S05-1046WT
AS12S05-1546WT
AS12S05-2546WT
AS12S05-1506WT
AS12S05-2506WT
AS12S11-1546WT
AS12S11-2546WT
Particle size
m
S-5
S-10
120
120
10150
10250
20100
20150
20250
20100
20150
20250
Product number
AS12S05-1510WT
AS12S05-2510WT
AS12S05-1020WT
AS12S05-1520WT
AS12S05-2520WT
AS12S11-1020WT
AS12S11-1520WT
AS12S11-2520WT
See P142 for preparative columns other than those listed above.
Guard cartridge columns (with inner diameter 1.0, 1.5, or 2.0 mm: 2/pack, 4.0 mm: 3/pack)
AS12S03-0101CC
1.010
S-3
120
S-5
120
1.510
2.010
4.023
1.010
1.510
2.010
4.023
AS12S03-01P5CC
AS12S03-0102CC
AS12S03-G304CC
AS12S05-0101CC
AS12S05-01P5CC
AS12S05-0102CC
AS12S05-G304CC
ODS
S-5
A guard cartridge holder will need to be purchased separately before using this
product for the first time.
Cartridge holder
Cartridge holder
YMC guard cartridge pack

S-3
4.023
120
S-5
4.023
120
XPCHSMW
XPCHW
AS12S03-G304CCPK
AS12S05-G304CCPK
A guard cartridge pack includes a cartridge holder (1 set), a guard cartridge

column, and a column coupler (produced by PEEK).
Guard columns
S-5
120
S-10
120
1030
2050
AS12S05-0310WTG
AS12S05-0520WTG
2050
AS12S11-0520WTG
When columns packed with material from different lots are needed for validation
purposes, please contact us.
35
01.indd 35
11.1.28 10:40:56 AM
Analytical columns
Hydrosphere C18
Strong retention of hydrophilic compounds
Can be used with 100% water mobile phase

T
wo types of test reports are included with
each column
Pore size120
Carbon content12
Hydrophilic ODS
Hydrosphere C18 is designed to maintain adequate hydrophilicity on
the packing surface for superior separation of hydrophilic compounds.
Hydrophilic compounds are retained with much greater affinity than
with conventional ODS columns, permitting the product to be used
ODS
with 100% water eluent, a problematic eluent for conventional ODS

columns. Hydrosphere C18 is useful for separating a wide range of
compounds, including nucleic acids and their derivatives, organic
acids, saccharides, glycosides and peptides.
Appropriate for separation of hydrophilic compounds

Separation of organic acids
conventional ODS columnBrand
Hydrosphere C18
1
1
3
3,4
A000310E
High hydrophilicity of the

Hydrosphere C18 packing
material surface enables
longer retention and superior
separation of citric acid and
fumaric acid as compared to
a conventional ODS column
(Brand ).
10
min
A000615A
10
Column
1504.6 mm I.D.
Eluent
20 mM NaH2PO4-H3PO4 (pH 2.8)
Temperature 30
1. Lactic acid
2. Acetic acid
3. Citric acid
4. Fumaric acid
5. Succinic acid
min
Reproducibility of retention time when used with 100% water mobile phase
Hydrosphere C18
Before standing
10
20 min
S0517A
10
S0518H
After standing
S0515A
2
1
10
98
20 min
S0519A
20
min
S0518F
10
89
10
20
min
S0519C
10
20 min
71
20 min
After completion of analysis, the flow was stopped and the columns were left to stand overnight (ca. 15 hours).
1. Cytosine
2. Uracil
3. Guanine
4. Thymine
5. Adenine
Column
1504.6 mm I.D.
Eluent
20 mM KH2PO4-K2HPO4 (pH 6.9)
Temperature 37
When conventional ODS columns are

used with 100% water mobile phase,
the apparent hydrophobicity decreases
due to repulsive interactions between
water and hydrophobic groups on
the surface of the packing materials,
reducing retention of compounds.
In the experiment illustrated here,
columns filled with 100% water mobile
phase were left to stand overnight.
Retention times of nucleic acid bases
were analyzed before and after
standing and compared. The retention
times of adenine after standing
decreased to 89% of the original
value for Brand II and to 71% of the
original Brand III value. By contrast,
Hydrosphere C18 retained 98% of
its original retention for adenine.
Hydrosphere C18 is designed to
maintain adequate hydrophilicity
on the packing surface so that
hydration can be achieved, and when
Hydrosphere C18 is used with mobile
phase containing no organic solvent,
the retention time is not significantly
shortened and highly reproducible
chromatograms can be obtained.
36
01.indd 36
11.1.28 10:41:01 AM
Differences in separation characteristics from conventional ODS columns

Hydrosphere C18
10
S000309A
20
30
40
min
C18
2
1
10
S990607B
20
30
40
min
ODS
The chromatograms shown were obtained in tests

conducted to compare separation characteristics using
a mixed sample containing compounds with different
characteristics.
The packing material surface of Hydrosphere C18 has
higher hydrophilicity and lower C18 ligand density than
those of Pro C18. This results in shorter retention of
hydrophobic compounds, including n-propylbenzene
and n-butylbenzene, and planar compounds like
acenaphthene. By contrast, retention of testosterone,
a compound with large hydrogen-bonding properties, is
longer on Hydrosphere C18 and on Brand IV, which also
has a highly hydrophilic packing surface.
Amitriptyline, a basic compound, caused tailing peaks
in Brand IV and Brand V. By contrast, Hydrosphere C18
and Pro C18 produce good peak shapes for such basic
compounds. Although the retention times of amitriptyline
on Hydrosphere C18 and Pro 18 are similar, there is a
big difference between the two columns with regard to
resolution of n-butylbenzene and amitriptyline. This
difference is considered to be due to the hydrophilicity of
the Hydrosphere C18 surface.
These results demonstrate that Hydrosphere C18, having
different separation characteristics from conventional
ODS columns, enables separation of compounds that
cannot be separated using other ODS columns.
Brand
1
2
5
6
10
F000614B
20
30
40
50
60
min
Brand
5
6
F000614C
10
20
30
40
50
1. Toluene
2. Testosterone
3. n-Propylbenzene
4. Acenaphthene
5. n-Butylbenzene
6. Amitriptyline hydrochloride
60
70
80
90
min
Column
1504.6 mm I.D.
Eluent
20 mM KH2PO4-K2HPO4 (pH 6.9)/methanol (35/65)
Temperature 37
37
01.indd 37
11.1.28 10:41:04 AM
Analytical columns
Hydrosphere C18
Short analysis time

conventional ODS column
Hydrosphere C18
2 4
2 4
ODS
Hydrosphere C18 can reduce the time necessary

for analyzing compounds with high hydrophobicity
by about 20% as compared with conventional ODS
columns.
Antioxidants
1. Nordihydroguaiaretic acid (NDGA)

2. Butyl hydroxyanisol (BHA)
3. 4-Hydroxy-2,6-di-tert-butylphenol (HMBP)
4. n-Octyl gallate (OG)
5. Dibutyl hydroxytoluene (BHT)
6. n-Dodesyl gallate (DG)
J010216B
10
15
min
J010215B
10
15
min
Column
504.6 mm I.D. (3 m, 120 )
Eluent
acetonitrile/methanol/5% acetic acid (35/35/30)
Temperature 30
Application
F000707B
Nicotinic acid and derivatives

4
1 2
1. Nicotinamide adenine dinucleotide phosphate oxidized form (NADP)

2. Nicotinic acid
3. Nicotinamide adenine dinucleotide phosphate reduced form (NADPH)
4. Nicotinamide
5. -Nicotinamide adenine dinucleotide (NAD)
6. -Nicotinamide adenine dinucleotide reduced form (NADH)
Column
Hydrosphere C18 (5 m, 120 )

1504.6 mm I.D.
Eluent
Temperature 37
10
15
20
25
min
Application
A000922A
Nucleotides
5
1. 5-UTP
2. 5-UDP
3. 5-CMP
4. 5-UMP
5. 5-dCMP
6. 5-GMP
1
7
2
10
Column
Hydrosphere C18 (5 m, 120 )

1504.6 mm I.D.
Eluent
Temperature 30
11
10
15
7. 5-ATP
8. 5-IMP
9. 5-ADP
10. 5-TMP
11. 5-AMP
20 min
38
01.indd 38
11.1.28 10:41:07 AM
Analytical columns
Particle size
m
S-3
120
120
1.0 35
1.0 50
1.0100
1.5 35
1.5 50
1.5100
2.0 35
2.0 50
2.0 75
2.0100
2.0150
3.0 50
3.0100
3.0150
4.6 35
4.6 50
4.6 75
4.6100
4.6150
6.0 50
6.0 75
6.0100
1.0150
1.0250
1.5150
1.5250
2.0 35
2.0 50
2.0 75
2.0100
2.0150
2.0250
3.0 50
3.0 75
3.0100
3.0150
3.0250
4.6 50
4.6 75
4.6100
4.6150
4.6250
6.0150
6.0250
Product number
HS12S03-H501WT
HS12S03-0501WT
HS12S03-1001WT
HS12S03-H5P5WT
HS12S03-05P5WT
HS12S03-10P5WT
HS12S03-H502WT
HS12S03-0502WT
HS12S03-L502WT
HS12S03-1002WT
HS12S03-1502WT
HS12S03-0503WT
HS12S03-1003WT
HS12S03-1503WT
HS12S03-H546WT
HS12S03-0546WT
HS12S03-L546WT
HS12S03-1046WT
HS12S03-1546WT
HS12S03-0506WT
HS12S03-L506WT
HS12S03-1006WT
HS12S05-1501WT
HS12S05-2501WT
HS12S05-15P5WT
HS12S05-25P5WT
HS12S05-H502WT
HS12S05-0502WT
HS12S05-L502WT
HS12S05-1002WT
HS12S05-1502WT
HS12S05-2502WT
HS12S05-0503WT
HS12S05-L503WT
HS12S05-1003WT
HS12S05-1503WT
HS12S05-2503WT
HS12S05-0546WT
HS12S05-L546WT
HS12S05-1046WT
HS12S05-1546WT
HS12S05-2546WT
HS12S05-1506WT
HS12S05-2506WT
Particle size
m
S-5
120
10150
10250
20100
20150
20250
Product number
HS12S05-1510WT
HS12S05-2510WT
HS12S05-1020WT
HS12S05-1520WT
HS12S05-2520WT
HS12S03-0101CC
1.010
S-3
120
S-5
120
1.510
2.010
4.023
1.010
1.510
2.010
4.023
HS12S03-01P5CC
HS12S03-0102CC
HS12S03-G304CC
HS12S05-0101CC
HS12S05-01P5CC
HS12S05-0102CC
HS12S05-G304CC
Cartridge holder
Cartridge holder

S-3
4.023
120
S-5
4.023
120
ODS
S-5
XPCHSMW
XPCHW
HS12S03-G304CCPK
HS12S05-G304CCPK

Guard columns
S-5
120
1030
2050
HS12S05-0310WTG
HS12S05-0520WTG
39
01.indd 39
11.1.28 10:41:08 AM
Analytical columns
YMC-Pack Pro C18 RS

E
xcellent acid resistance and alkali
resistance (pH 1 to 10)
Superior separation of structural isomers
T
wo types of test reports are included
Pore size80
Carbon content22
with each column
Highly durable ODS

YMC-Pack Pro C18 RS is a polymeric type high carbon ODS
column characterized by high resolution and high durability.
It is applicable to a wide range of compounds, providing
good separation of basic compounds that easily cause
Excellent durability
The
pH 1
C18 RS packing material hydrophobicity is hardly changed
Unlike conventional ODS columns,
pH 10
100
100
80
80
60
60
20
40
( )
YMC-Pack Pro C18 RS

Acid-resistant column
(manufactured by other company)
Monomeric ODS
40
C18 RS
can be used with an alkali mobile phase.
under the harsh conditions of pH1 and column temperature 70 .
ODS
tailing peaks. The separation selectivity for compounds

that differ only slightly in hydrophobicity is outstanding. It
also has excellent resistance to acid and alkali, making it
useful under demanding separation conditions.
Pro C18 RS
Monomeric ODS
20
0
20
40
60
80
100(hours)
Hours exposure to acetonitrile/water/TFA (10/90/1),
change in packing material hydrophobicity at 70 .
0
200
400
600
800(hours)
Sequential analysis using 20 mM H3BO3-NaOH (pH 9.8)/methanol (50/50)
A wide choice of mobile phases facilitates method development

1504.6 mmI.D. 5 m
pH 3.2
3,4,5
504.6 mmI.D. 3 m
pH 6.8
pH 8.8
pH 8.8
Downsizing
1 2
12
F040716A
10
1. Flurbiprofen
2. Methyl benzoate
3. Lidocaine
4. Diltiazem
5. Verapamil
15
12 3 4
20 min
F040714E
10
15
20 min
Column
YMC-Pack Pro C18 RS
Eluent
20 mM acetate buffer/methanol (35/65)
Temperature 30
F040714C
10
15
20 min
F040714D
10 min
Pro C18 RS with excellent acid and alkali resistance can

be used over a wide pH range, and it is easy to optimize the
separating conditions. In addition, by downsizing from the
column (particle 5 m, length 150 mm) to a shorter column
(particle 3 m, length 50 mm), with high separation ability, it
becomes possible to shorten the analysis time by one third
without affecting the separation.
40
01.indd 40
11.1.28 10:41:12 AM
Separation of basic compounds

YMC-Pack
C18 RS
Acid-resistant column manufactured

by other companyBrand D3
Tf(4) 2.87
3
1
0
10
J011114B
20 min
10
J020523D
20 min
1. Desipramine
2. Diphenyl (I.S.)
3. Imipramine
4. Amitriptyline
Column
1504.6 mmI.D.
Eluent

/methanol (25/75)
Temperature 37
Separation of structural isomers

Pro C18 RS with a high C18 density is excellent
in hydrophobicity recognition ability and plane
recognition ability, and can be applied to
separate structural isomers which are difficult
to separate with other ODS. In addition, by
using 3 mcolumn packing, resolution can be
increased, and it becomes possible to achieve
a baseline separation of linolenate isomers.
C18 RS
5 m
Brand F1
(standard-type ODS 5 m)
ODS
Tf(4) 1.16
Amitriptyline is a basic compound that can

easily tail on some silica based columns. In
comparison with competing acid-resistant
columns, Pro C18 RS produces good peak
shapes without tailing.
Rs=1.24
Rs=0.8
F040713D
10
20
30
min
0 F040714
10
20
30 min
Methyl linolenate isomers

1. Methyl linolenate
2. Methyl -linolenate
Column
1504.6 mmI.D.
Eluent
acetonitrile/THF/water (35/30/35)
Temperature 30
C18 RS
3 m
1
Rs=1.54
F040713A
10
20
30 min
41
01.indd 41
11.1.28 10:41:15 AM
Analytical columns
YMC-Pack Pro C18 RS
Separation of peptides by LC/MS
530.95
M+2
100
%
ODS
Pro C18 RS produces good peak shapes when

it is used with mobile phase containing low
TFA concentration, making it suitable for LC/
MS.
Bradykinin(MW: 1059.56)
531.47
M+1
1060.27
400
600
800
100
18
5.00
1000
3 5
1200(m/z)
1. [Arg8]-Vasopressin
2. Bradykinin
3. Angiotensin
4. Oxytocin
5. Angiotensin
6. Leu-Enkephalin
10.00
15.00
Column
YMC-Pack Pro C18 RS (5 m, 80 )

1502.0 mmI.D.
Eluent
A) water/TFA (100/0.01)


10-35% B (0-15 min)
Temperature 37
Detection ESI positive-mode
20.00
Time
Application
Chloroacetophenone isomers
1.
Chlorophenol isomers
COCH3
Cl
1.
o-Chloroacetophenone
2.
3.
2.
J011026B
10
20 min
3.
Cl
m-Chloroacetophenone
OH
Cl
p-Chlorophenol
COCH3
Column

1504.6 mmI.D.
Eluent
Temperature 30
Cl
o-Chlorophenol
COCH3
Cl
p-Chloroacetophenone
OH
OH
Cl
m-Chlorophenol
Column

Eluent
J011113D
10
20 min

1504.6 mmI.D.
a cetonitrile/water/acetic acid
(30/70/1)
Temperature 37
42
01.indd 42
11.1.28 10:41:19 AM
Analytical columns
Particle size
m
S-3
80
80
1.0 35
1.0 50
1.0100
1.5 35
1.5 50
1.5100
2.0 35
2.0 50
2.0 75
2.0100
2.0150
3.0 50
3.0100
3.0150
4.6 35
4.6 50
4.6 75
4.6100
4.6150
6.0 50
6.0 75
6.0100
1.0150
1.0250
1.5150
1.5250
2.0 35
2.0 50
2.0 75
2.0100
2.0150
2.0250
3.0 50
3.0 75
3.0100
3.0150
3.0250
4.6 50
4.6 75
4.6100
4.6150
4.6250
6.0150
6.0250
Product number
RS08S03-H501WT
RS08S03-0501WT
RS08S03-1001WT
RS08S03-H5P5WT
RS08S03-05P5WT
RS08S03-10P5WT
RS08S03-H502WT
RS08S03-0502WT
RS08S03-L502WT
RS08S03-1002WT
RS08S03-1502WT
RS08S03-0503WT
RS08S03-1003WT
RS08S03-1503WT
RS08S03-H546WT
RS08S03-0546WT
RS08S03-L546WT
RS08S03-1046WT
RS08S03-1546WT
RS08S03-0506WT
RS08S03-L506WT
RS08S03-1006WT
RS08S05-1501WT
RS08S05-2501WT
RS08S05-15P5WT
RS08S05-25P5WT
RS08S05-H502WT
RS08S05-0502WT
RS08S05-L502WT
RS08S05-1002WT
RS08S05-1502WT
RS08S05-2502WT
RS08S05-0503WT
RS08S05-L503WT
RS08S05-1003WT
RS08S05-1503WT
RS08S05-2503WT
RS08S05-0546WT
RS08S05-L546WT
RS08S05-1046WT
RS08S05-1546WT
RS08S05-2546WT
RS08S05-1506WT
RS08S05-2506WT
Particle size
m
S-5
80
10150
10250
20100
20150
20250
Product number
RS08S05-1510WT
RS08S05-2510WT
RS08S05-1020WT
RS08S05-1520WT
RS08S05-2520WT
RS08S03-0101CC
1.010
S-3
80
S-5
80
1.510
2.010
4.023
1.010
1.510
2.010
4.023
RS08S03-01P5CC
RS08S03-0102CC
RS08S03-G304CC
RS08S05-0101CC
RS08S05-01P5CC
RS08S05-0102CC
RS08S05-G304CC
Cartridge holder
Cartridge holder

S-3
80
4.023
S-5
80
4.023
ODS
S-5
XPCHSMW
XPCHW
RS08S03-G304CCPK
RS08S05-G304CCPK

Guard columns
S-5
80
1030
2050
RS08S05-0310WTG
RS08S05-0520WTG
43
01.indd 43
11.1.28 10:41:20 AM
Analytical columns
YMC-Pack ODS-A
Conventional ODS column
Currently in use worldwide
Pore size120 , 200 , 300

Carbon content17 (120 ), 12 (200 ), 7 (300 )
Standard ODS
YMC-Pack ODS-A has a highly endcapped surface
structure and appropriate hydrophobicity for separation
of a wide range of compounds. It is produced under strict
quality control with respect to 50 or more parameters in
Quality control system for excellent performance

The graph indicates lot-to-lot reproducibility with respect
to hydrophobic interaction for 10 lots, an essential column
performance characteristic. Extremely stable lot-to-lot
reproducibility of the hydrophobic surface is achieved by strict
control of variables.
In addition to measurement of the physical properties, a wide
range of compounds including hydrophobic, acidic, basic and
coordination compounds are analyzed under optimal conditions
to evaluate column performance.
10
tR (Butyl benzoate), min
ODS
order to ensure stable quality.

This product is highly regarded as the standard YMCPack packing material in various countries around the
world.
8
6
4
2
0
4 5 6 7
Lot Number
9 10
Application
S930422D
Application
Analgesics
O
CH3COHN
OH
OC2H5
Ethenzamide
CH3
N
N
N
CH3
1.-Carotene
2.-Carotene
Caffeine
CONH2
H 3C
O
Acetaminophen
Carotenes
CH2= CHCH2
H 3C
H 3C
CHCHCONHCONH 2
Apronalide
10
20
30 min
G910523Q
Column
YMC-Pack ODS-A

(5 m, 120 )

1504.6 mmI.D.
Eluent
Temperature 30
10
20
min
Column
YMC-Pack ODS-A

(5 m, 120 )

1504.6 mmI.D.
Eluent
acetonitrile/methanol/THF

(50/45/5)
Temperature 30
Analytical columns
Particle size
m
S-3
120
2.0 35
2.0 50
2.0 75
2.0100
2.0150
3.0 50
3.0100
3.0150
Product number
AA12S03-H502WT
AA12S03-0502WT
AA12S03-L502WT
AA12S03-1002WT
AA12S03-1502WT
AA12S03-0503WT
AA12S03-1003WT
AA12S03-1503WT
Particle size
m
S-3
120
4.6 75
4.6100
4.6150
6.0100
6.0150
Product number
AA12S03-L546WT
AA12S03-1046WT
AA12S03-1546WT
AA12S03-1006WT
AA12S03-1506WT
44
01.indd 44
11.1.28 10:41:23 AM
Analytical columns
Particle size
m
S-5
120
300
S-10
120
300
AA12S05-1501WT
AA12S05-2501WT
AA12S05-15P5WT
AA12S05-25P5WT
AA12S05-L502WT
AA12S05-1502WT
AA12S05-2502WT
AA12S05-1503WT
AA12S05-2503WT
AA12S05-L546WT
AA12S05-1046WT
AA12S05-1546WT
AA12S05-2546WT
AA12S05-3046WT
AA12S05-1006WT
AA12S05-1506WT
AA12S05-2506WT
AA12S05-3006WT
AA20S05-1546WT
AA20S05-2546WT
AA30S05-1501WT
AA30S05-2501WT
AA30S05-15P5WT
AA30S05-25P5WT
AA30S05-L502WT
AA30S05-1502WT
AA30S05-2502WT
AA30S05-L546WT
AA30S05-1046WT
AA30S05-1546WT
AA30S05-2546WT
AA30S05-3046WT
AA30S05-1006WT
AA30S05-1506WT
AA30S05-2506WT
AA30S05-3006WT
AA12S11-1046WT
AA12S11-1546WT
AA12S11-2546WT
AA12S11-3046WT
AA12S11-1006WT
AA12S11-1506WT
AA12S11-2506WT
AA12S11-3006WT
AA30S11-1046WT
AA30S11-1546WT
AA30S11-2546WT
AA30S11-3046WT
AA30S11-1006WT
AA30S11-1506WT
AA30S11-2506WT
AA30S11-3006WT
Particle size
m
S-10
S-5
120
200
300
10150
10250
10300
20100
20150
20250
20150
20250
10150
10250
10300
20150
20250
120
200
300
AA12S11-1510WT
AA12S11-2510WT
AA12S11-3010WT
AA12S11-1520WT
AA12S11-2520WT
AA20S11-1520WT
AA20S11-2520WT
AA30S11-1510WT
AA30S11-2510WT
AA30S11-3010WT
AA30S11-1520WT
AA30S11-2520WT
AA12S03-0102CC
2.010
S-3
S-5
120
200
300
4.023
1.010
1.510
2.010
4.023
4.023
1.010
1.510
2.010
4.023
AA12S03-G304CC
AA12S05-0101CC
AA12S05-01P5CC
AA12S05-0102CC
AA12S05-G304CC
AA20S05-G304CC
AA30S05-0101CC
AA30S05-01P5CC
AA30S05-0102CC
AA30S05-G304CC
Cartridge holder
Cartridge holder
XPCHSMW
XPCHW

S-3
120
4.023
AA12S03-G304CCPK
S-5
120
4.023
AA12S05-G304CCPK

Guard columns
S-3
120
S-5
120
200
Product number
AA12S05-1510WT
AA12S05-2510WT
AA12S05-3010WT
AA12S05-1020WT
AA12S05-1520WT
AA12S05-2520WT
AA20S05-1520WT
AA20S05-2520WT
AA30S05-1510WT
AA30S05-2510WT
AA30S05-3010WT
AA30S05-1520WT
AA30S05-2520WT
10150
10250
10300
20150
20250
20150
20250
10150
10250
10300
20150
20250
Product number
Particle size
m
ODS
200
1.0150
1.0250
1.5150
1.5250
2.0 75
2.0150
2.0250
3.0150
3.0250
4.6 75
4.6100
4.6150
4.6250
4.6300
6.0100
6.0150
6.0250
6.0300
4.6150
4.6250
1.0150
1.0250
1.5150
1.5250
2.0 75
2.0150
2.0250
4.6 75
4.6100
4.6150
4.6250
4.6300
6.0100
6.0150
6.0250
6.0300
4.6100
4.6150
4.6250
4.6300
6.0100
6.0150
6.0250
6.0300
4.6100
4.6150
4.6250
4.6300
6.0100
6.0150
6.0250
6.0300
Product number
300
S-10
120
200
300
4.010
5.010
4.010
5.010
1030
2050
4.010
2050
4.010
5.010
1030
2050
4.010
5.010
1030
2050
4.010
5.010
2050
4.010
5.010
1030
2050
AA12S03-0104WFG
AA12S03-0105WFG
AA12S05-0104WFG
AA12S05-0105WFG
AA12S05-0310WTG
AA12S05-0520WTG
AA20S05-0104WFG
AA20S05-0520WTG
AA30S05-0104WFG
AA30S05-0105WFG
AA30S05-0310WTG
AA30S05-0520WTG
AA12S11-0104WFG
AA12S11-0105WFG
AA12S11-0310WTG
AA12S11-0520WTG
AA20S11-0104WFG
AA20S11-0105WFG
AA20S11-0520WTG
AA30S11-0104WFG
AA30S11-0105WFG
AA30S11-0310WTG
AA30S11-0520WTG
45
01.indd 45
11.1.28 10:41:24 AM
Analytical columns
YMC-Pack ODS-AM
Similar to ODS-A in selectivity
Useful for quality control purposes
Pore size120
Carbon content17
ODS with outstanding lot-to-lot reproducibility

YMC-Pack ODS-AM is a product which especially
emphasizes lot-to-lot reproducibility of the packing
material. Strict control is performed on all aspects of
quality, including physical properties of silica gel base
Special attention is given to lot-to-lot reproducibility

10
(Methylparben/2,6-Dimethylpyridine)
ODS
and surface modifying processes, in order to ensure

stable quality. Double endcapping is conducted to
ensure that lot-to-lot reproducibility is maintained.
The separation factor () of methylparaben/2,6dimethylpyridine for 10 lots of ODS-AM packing material

is plotted on the left graph. Excellent lot-to-lot
reproducibility is achieved even in separation of basic
compounds.
10
Lot Number
Acidic compounds
1. Uracil
2. Benzoic acid
3. p-Toluic acid
4. p-Ethylbenzoic acid
5. Methy benzoate
1. Uracil
2. Acetylacetone
3. 8-Quinolinol
ODS-AM
3
2
ODS with a high degree of

metal impurities
1
2
4
Secondary interaction of the stationary phase surface may

affect peak shapes of acidic compounds.
ODS-AM can provide chromatograms without tailing peaks as
shown above.
The peak shapes of coordination compounds, including

8-quinolinol and acetylacetone, may be affected by the
presence of metal impurities on the surface of the silica. The
distribution level of metal impurities on the column is strictly
controlled for ODS-AM, providing excellent peak symmetry.
46
01.indd 46
11.1.28 10:41:26 AM
Application
G910523J
Alkaloids
1
4
3
7
5
9
8
6
20
40
60
80
min
Column
YMC-Pack ODS-AM (5 m, 120 )

1504.6 mmI.D.
Eluent
acetonitrile/20 mM KH2PO4 (20/80)
Temperature 30
Analytical columns
Particle size
m
S-3
S-5
120
120
2.0 35
2.0 50
2.0 75
2.0100
2.0150
3.0 50
3.0100
3.0150
4.6 75
4.6100
4.6150
6.0100
6.0150
6.0250
1.0150
1.0250
1.5150
1.5250
2.0 75
2.0100
2.0150
2.0250
3.0150
3.0250
4.6 75
4.6100
4.6150
4.6250
4.6300
6.0100
6.0150
6.0250
6.0300
10150
S-5
120
10250
10300
20100
20150
20250
Product number
AM12S03-H502WT
AM12S03-0502WT
AM12S03-L502WT
AM12S03-1002WT
AM12S03-1502WT
AM12S03-0503WT
AM12S03-1003WT
AM12S03-1503WT
AM12S03-L546WT
AM12S03-1046WT
AM12S03-1546WT
AM12S03-1006WT
AM12S03-1506WT
AM12S03-2506WT
AM12S05-1501WT
AM12S05-2501WT
AM12S05-15P5WT
AM12S05-25P5WT
AM12S05-L502WT
AM12S05-1002WT
AM12S05-1502WT
AM12S05-2502WT
AM12S05-1503WT
AM12S05-2503WT
AM12S05-L546WT
AM12S05-1046WT
AM12S05-1546WT
AM12S05-2546WT
AM12S05-3046WT
AM12S05-1006WT
AM12S05-1506WT
AM12S05-2506WT
AM12S05-3006WT
AM12S05-1510WT
AM12S05-2510WT
AM12S05-3010WT
AM12S05-1020WT
AM12S05-1520WT
AM12S05-2520WT
01.indd 47
Particle size
m
S-3
120
S-5
120
2.010
4.023
1.010
1.510
2.010
4.023
ODS
1. Hordenine sulfate
2. Pilocarpine chloride
3. Scopolamine bromide
4. Atropine sulfate
5. Cinchonine
6. Sparteine sulfate
7. Quinine
8. Papaverine chloride
9. Narcotine chloride
Product number
AM12S03-0102CC
AM12S03-G304CC
AM12S05-0101CC
AM12S05-01P5CC
AM12S05-0102CC
AM12S05-G304CC
Cartridge holder
Cartridge holder
XPCHSMW
XPCHW

S-3
120
4.023
AM12S03-G304CCPK
S-5
120
4.023
AM12S05-G304CCPK

Guard columns
S-3
120
S-5
120
4.010
5.010
4.010
5.010
1030
2050
AM12S03-0104WFG
AM12S03-0105WFG
AM12S05-0104WFG
AM12S05-0105WFG
AM12S05-0310WTG
AM12S05-0520WTG
47
11.1.28 10:41:27 AM
Analytical columns
YMC-Pack ODS-AQ
Useful for separation of hydrophilic compounds
Can be used with 100% water in mobile phase
Different selectivity from conventional ODS
Pore size120 , 200

Carbon content14 , 10%
Hydrophilic ODS
YMC-Pack ODS-AQ has moderate hydrophobicity and
hydrogen-bonding capacity. It shows different retention
behavior from that of YMC-Pack ODS-A, for samples
with relatively high hydrophilicity.
ODS
It is useful in fields including carbohydrate chemistry for

oligosaccharides and glycosides, pharmacognosy and
natural product chemistry.
Useful for separation of sugars

This product is useful for separations using mobile
phases in which water content is relatively high, such as
separation of sugars and glycosides.
G11
G12
G13
1. MaltoundecaoseG11
2. MaltododecaoseG12
3. MaltotridecaoseG13
4. MaltotetradecaoseG14
5. MaltopentadecaoseG15
G14
G15
10
20
30
Column
YMC-Pack ODS-AQ (5 m, 120 )

1504.6 mmI.D.
Eluent
Temperature 37
Detection RI, 810-6RIU/FS
min
M910604M
Hydrogen-bonding capacity
ODS-A
ODS-AQ
ODS-AL
2
3
3
4
Chromatograms of purine alkaloids are shown on the left.

As compared with ODS-A, ODS-AQ and ODS-AL show
longer retention of caffeine and theophylline relative to
phenol, an internal standard.
5
4
1. Trigonelline
2. Theobromine
3. Theophylline
4. Caffeine
5. Phenol
(Phenol/Caffeine)
1.8
ODS-A
1.6
1.4
ODS-AQ
1.2
ODS-AL
Change of separation factor () of caffeine/phenol

according to temperature change in ODS-AQ, ODS-A
and ODS-AL is plotted on the graph shown on the left.
Although caffeine is considered to have stronger hydrogenbonding properties than phenol, the hydrogen-bonding
capacity of stationary phase may affect the of caffeine/
phenol. The smaller the hydrogen-bonding capacities
are, the higher of phenol/caffeine. ODS-AQ shows
different retention behavior from that of ODS-A since it has
moderate hydrogen-bonding capacity.
1.0
3.0
(60 )
3.1
3.2
3
10 /T,K
3.3
-1
3.4
(20 )
48
01.indd 48
11.1.28 10:41:30 AM
Application
G910607E
Crude drugs (glycosides)

1
1. Arbutin
2. Salicin
3. Esculine
4. G-Strophantine(Ouabain)
5. Baicalin
6. Barbaloin
7. Glycyrrhizin
8. Digitoxine
5
34
2
7
8
10
20
30
40
min
Analytical columns
Particle size
m
S-3
S-5
120
120
200
2.0 35
2.0 50
2.0 75
2.0100
2.0150
3.0 50
3.0100
3.0150
4.6100
4.6150
6.0100
6.0150
1.0150
1.0250
1.5150
1.5250
2.0 75
2.0150
2.0250
3.0150
3.0250
4.6 75
4.6100
4.6150
4.6250
4.6300
6.0100
6.0150
6.0250
6.0300
4.6150
4.6250
10150
S-5
120
200
S-10
120
200
10250
10300
20100
20150
20250
20150
20250
10150
10250
10300
20150
20250
20150
20250
Product number
AQ12S03-H502WT
AQ12S03-0502WT
AQ12S03-L502WT
AQ12S03-1002WT
AQ12S03-1502WT
AQ12S03-0503WT
AQ12S03-1003WT
AQ12S03-1503WT
AQ12S03-1046WT
AQ12S03-1546WT
AQ12S03-1006WT
AQ12S03-1506WT
AQ12S05-1501WT
AQ12S05-2501WT
AQ12S05-15P5WT
AQ12S05-25P5WT
AQ12S05-L502WT
AQ12S05-1502WT
AQ12S05-2502WT
AQ12S05-1503WT
AQ12S05-2503WT
AQ12S05-L546WT
AQ12S05-1046WT
AQ12S05-1546WT
AQ12S05-2546WT
AQ12S05-3046WT
AQ12S05-1006WT
AQ12S05-1506WT
AQ12S05-2506WT
AQ12S05-3006WT
AQ20S05-1546WT
AQ20S05-2546WT
Particle size
m
S-3
120
S-5
120
200
2.010
4.023
1.010
1.510
2.010
4.023
4.023
Product number
AQ12S03-0102CC
AQ12S03-G304CC
AQ12S05-0101CC
AQ12S05-01P5CC
AQ12S05-0102CC
AQ12S05-G304CC
AQ20S05-G304CC
Cartridge holder
Cartridge holder
XPCHSMW
XPCHW

S-3
120
4.023
AQ12S03-G304CCPK
S-5
120
4.023
AQ12S05-G304CCPK

Guard columns
S-3
120
S-5
AQ12S05-1510WT
AQ12S05-2510WT
AQ12S05-3010WT
AQ12S05-1020WT
AQ12S05-1520WT
AQ12S05-2520WT
AQ20S05-1520WT
AQ20S05-2520WT
AQ12S11-1510WT
AQ12S11-2510WT
AQ12S11-3010WT
AQ12S11-1520WT
AQ12S11-2520WT
AQ20S11-1520WT
AQ20S11-2520WT
ODS
Column
YMC-Pack ODS-AQ (5 m, 120 )

2504.6 mmI.D.
Eluent
A) methanol/20 mM NH4H2PO4 (5/95)

B) methanol/20 mM NH4H2PO4 (80/20)

0-100 B (0-20 min,linear),100 B (20-40 min)
Temperature 30
120
200
S-10
120
200
4.010
5.010
4.010
5.010
1030
2050
4.010
2050
1030
2050
2050
AQ12S03-0104WFG
AQ12S03-0105WFG
AQ12S05-0104WFG
AQ12S05-0105WFG
AQ12S05-0310WTG
AQ12S05-0520WTG
AQ20S05-0104WFG
AQ20S05-0520WTG
AQ12S11-0310WTG
AQ12S11-0520WTG
AQ20S11-0520WTG
49
01.indd 49
11.1.28 10:41:32 AM
Analytical columns
YMC-Pack ODS-AL
ODS with residual silanol groups
Utilizes secondary interaction caused by silanol groups
Pore size120
Carbon content17
Non-endcapped ODS
YMC-Pack ODS-AL uses not only hydrophobic
interaction but also secondary interaction caused by
silanol groups that affect separation. This results in a
different selectivity from conventional ODS columns.
ODS
When ionic interaction is utilized, it is preferable to use

a buffer in the mobile phase to achieve reproducibility of
chromatograms.
Effects of silanol group

ODS-AL
The separation factor () of internal standards

methylparaben/2,6-dimethylpyridine for ODS-AM is
different from that of ODS-AL, which is not endcapped.
ODS-AL, which is affected by residual silanol, shows
relatively great retention of pyridines.
ODS-AM
2 3
3
(4/3)=2.80
(4/3)=1.46
1. Uracil
2. Pyridine
3. 2,6-Dimethylpyridine
4. Methylparaben
4
4
Utilizes residual silanol groups for separation

ODS-AQ
ODS-AL
The figure shows separation of disinfectants under the

conditions described in the Japanese Pharmacopeia. The
object is to select the column permitting the elution of benzoic
acid, salicylic acid and theophylline, in this order, insuring
complete separation of these peaks. With ODS-AQ, separation
of salicylic acid and theophylline is incomplete. By contrast,
ODS-AL provides excellent separation. Thus, ODS-AL may
provide excellent separation when the separation conditions
cannot be optimized on other ODS columns.
2,3
3
1
Disinfectants
1.
COOH
2.
COOH
OH
Benzoic acid
Salicylic acid
3.
4.
H3C
H
N
OH
CH 3
Theophylline (I.S.)
10
15
20
min
10
15
20
min
Phenol
Column
YMC-Pack ODS-AL and ODS-AQ (5 m, 120 )

1504.6 mmI.D.
Eluent
100 mM KH2PO4-Na2HPO4 (pH 7.0)/methanol (75/25)
Temperature 30
(Conditions described in Japanese Pharmacopoeia 15th ed.)
50
01.indd 50
11.1.28 10:41:35 AM
Application
G900417B
Phenacyl esters of bile acids from pig gall

2
1. Cholic acid (CA)

2. Hyodeoxycholic acid (HDCA)
3. Chenodeoxycholic acid (CDAC)
ODS
Column
10
20
30
40
min
YMC-Pack ODS-AL (5 m, 120 )

2504.6 mmI.D.
Eluent
Temperature 40
Analytical columns
Particle size
m
S-5
120
1.0150
1.0250
1.5150
1.5250
2.0150
2.0250
4.6 75
4.6100
4.6150
4.6250
4.6300
6.0100
6.0150
6.0250
6.0300
10150
S-5
120
10250
10300
20100
20150
20250
Product number
AL12S05-1501WT
AL12S05-2501WT
AL12S05-15P5WT
AL12S05-25P5WT
AL12S05-1502WT
AL12S05-2502WT
AL12S05-L546WT
AL12S05-1046WT
AL12S05-1546WT
AL12S05-2546WT
AL12S05-3046WT
AL12S05-1006WT
AL12S05-1506WT
AL12S05-2506WT
AL12S05-3006WT
AL12S05-1510WT
AL12S05-2510WT
AL12S05-3010WT
AL12S05-1020WT
AL12S05-1520WT
AL12S05-2520WT
Particle size
m
S-5
120
1.010
1.510
2.010
4.023
Product number
AL12S05-0101CC
AL12S05-01P5CC
AL12S05-0102CC
AL12S05-G304CC
Cartridge holder
Cartridge holder
Guard columns
S-5
120
4.010
5.010
1030
2050
XPCHSMW
XPCHW
AL12S05-0104WFG
AL12S05-0105WFG
AL12S05-0310WTG
AL12S05-0520WTG
51
01.indd 51
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Analytical columns
Jsphere ODS-H80, ODS-M80, ODS-L80

Useful for method development
High theoretical plate number
Pore size80
Carbon contentODS-H80 22 , ODS-M80 14% , ODS-L80 9
Usable pH rangeODS-H80 1.0 9.0, ODS-M80L80 2.0 7.5
Three types of ODS, each with different ligand coverage

Jsphere offers a choice from three kinds of ODS made from the same
silica gel base, each with different ligand coverage.
Differences in ligand coverage considerably affect the hydrophobic
retention behavior of solutes, as well as the separation behavior
ODS
resulting from solute functional groups or tertiary structure.

Jsphere is useful for the optimization of separation conditions, since
there is almost no need to consider interactions other than hydrophobic
and hydrogen-bonding interactions (e.g., ionic or coordinate interaction).
Three types of ODS

Image of stationary phase
ODS-H80
ODS-M80
ODS-L80
CnH 2n+1 (n=0-8)

Alkylbenzenes
ODS-L80
ODS-M80
Column
J
sphere

1504.6 mmI.D.
Eluent
Temperature 37
ODS-H80
0
10
20
30
40
50 min
Differences in separation characteristics

Jsphere ODS-H80
BRAND-B1
16.5
BRAND-F1
15.9
YMC-Pack ODS-AM 15.2
BRAND-G1
15.2
Jsphere ODS-M80 15.1
BRAND-A2
15.0
BRAND-C3
13.1
6.2 Jsphere ODS-L80
0
10
20
k(octylbenzene)
0.21 Jsphere ODS-H80

BRAND-F1
0.24
BRAND-G1
0.24
YMC-Pack ODS-AM 0.25
BRAND-C3
0.25
BRAND-B1
0.25
0.27
Jsphere ODS-M80
BRAND-A2
Jsphere ODS-L80
0.20
0.25
(caffeine/phenol)
29.6
Column
1504.6 mmI.D.
Eluent
Flow rate
1.0 mL/min
Temperature 37
30
0.30
0.30
Column
1504.6 mmI.D.
Eluent
acetonitrile/20 mM KH2PO4 (15/85)
Flow rate
1.0 mL/min
Temperature 37
ODS columns with different

ligand coverage have different
hydrophobicity, hydrogen
bonding capacity and steric
selectivity for tertiary structures.
ODS-H80 has exceptionally high
hydrophobicity and low hydrogen
bonding capacity, ODS-M80 has
moderate hydrophobicity and
hydrogen bonding capacity and
ODS-L80 has low hydrophobicity,
comparable to that of C8 and
high hydrogen bonding capacity.
Jsphere can be used with
confidence to identify structural
differences of solutes and is useful
for improving the efficiency of
separation optimization.
0.30
52
01.indd 52
11.1.28 10:41:39 AM
Analytical columns
Packing Particle size Pore size Column size

material m inner diameterlengthmm
ODS-H80
ODS-L80
S-4
S-4
80
80
80
1.0150
1.0250
1.5150
1.5250
2.0 75
2.0100
2.0150
2.0250
3.0150
3.0250
4.6 75
4.6150
4.6250
6.0150
6.0250
1.0150
1.0250
1.5150
1.5250
2.0 75
2.0100
2.0150
2.0250
3.0150
3.0250
4.6 75
4.6150
4.6250
6.0150
6.0250
1.0150
1.0250
1.5150
1.5250
2.0 35
2.0 75
2.0100
2.0150
2.0250
3.0150
3.0250
4.6 75
4.6150
4.6250
6.0150
6.0250
JH08S04-1501WT
JH08S04-2501WT
JH08S04-15P5WT
JH08S04-25P5WT
JH08S04-L502WT
JH08S04-1002WT
JH08S04-1502WT
JH08S04-2502WT
JH08S04-1503WT
JH08S04-2503WT
JH08S04-L546WT
JH08S04-1546WT
JH08S04-2546WT
JH08S04-1506WT
JH08S04-2506WT
JM08S04-1501WT
JM08S04-2501WT
JM08S04-15P5WT
JM08S04-25P5WT
JM08S04-L502WT
JM08S04-1002WT
JM08S04-1502WT
JM08S04-2502WT
JM08S04-1503WT
JM08S04-2503WT
JM08S04-L546WT
JM08S04-1546WT
JM08S04-2546WT
JM08S04-1506WT
JM08S04-2506WT
JL08S04-1501WT
JL08S04-2501WT
JL08S04-15P5WT
JL08S04-25P5WT
JL08S04-H502WT
JL08S04-L502WT
JL08S04-1002WT
JL08S04-1502WT
JL08S04-2502WT
JL08S04-1503WT
JL08S04-2503WT
JL08S04-L546WT
JL08S04-1546WT
JL08S04-2546WT
JL08S04-1506WT
JL08S04-2506WT
Packing Particle size Pore size Column size

material m inner diameterlengthmm
ODS-H80
S-4
80
ODS-M80
S-4
80
ODS-L80
S-4
80
10150
10250
20150
20250
10150
10250
20150
20250
10150
10250
20150
20250
Product number
JH08S04-1510WT
JH08S04-2510WT
JH08S04-1520WT
JH08S04-2520WT
JM08S04-1510WT
JM08S04-2510WT
JM08S04-1520WT
JM08S04-2520WT
JL08S04-1510WT
JL08S04-2510WT
JL08S04-1520WT
JL08S04-2520WT
JH08S04-0101CC
1.010
ODS-H80 S-4
80
ODS-M80
S-4
80
ODS-L80
S-4
80
1.510
2.010
4.023
1.010
1.510
2.010
4.023
1.010
1.510
2.010
4.023
JH08S04-01P5CC
JH08S04-0102CC
JH08S04-G304CC
JM08S04-0101CC
JM08S04-01P5CC
JM08S04-0102CC
JM08S04-G304CC
JL08S04-0101CC
JL08S04-01P5CC
JL08S04-0102CC
JL08S04-G304CC
ODS
ODS-M80
S-4
Product number
Cartridge holder
Cartridge holder
XPCHSMW
XPCHW

ODS-H80 S-4
80
4.023
JH08S04-G304CCPK
ODS-M80
S-4
80
4.023
JM08S04-G304CCPK
ODS-L80
S-4
80
4.023
JL08S04-G304CCPK

Guard columns
ODS-H80 S-4
80
ODS-M80
S-4
80
ODS-L80
S-4
80
1030
2050
1030
2050
1030
2050
JH08S04-0310WTG
JH08S04-0520WTG
JM08S04-0310WTG
JM08S04-0520WTG
JL08S04-0310WTG
JL08S04-0520WTG
53
01.indd 53
11.1.28 10:41:40 AM
Analytical columns
YMC-Pack PolymerC18
Utilizes polymer base which is not affected by silanol
Different separation characteristics from silica-based ODS
Excellent pH stability
P o l y m e r t y p e C18
PolymerC18 is a C18 column made from methacrylate
polymer. It has excellent pH stability and it is useful for
separation of basic compounds because there are no
silanol or metal impurities to cause secondary interaction.
ODS
Since electrons of the carbonyl group or the hydroxyl

group on the surface of the base material show particular
interactions with solutes, PolymerC18 can show different
separation characteristics from silica-based ODS.
Can be used with alkali mobile phase

1
1. Barbital
2. Phenobarbital
3. Pentobarbital
4. Hexobarbital
5. Secobarbital
10
20 min
Column
YMC-Pack PolymerC18

1504.6 mmI.D.
Eluent
50 mM Na2HPO4-Na3PO4(pH 11.0)/methanol (75/25)
Temperature 30
Excellent resistance to acid and alkali

Alkali resistance
Acid resistance
Prior to eluent ow After 6 hours

Prior to eluent ow After 100 hours
2
2
3
10
20 min
10
2
3
20 min
Flow conditions
Column
YMC-Pack PolymerC18 1504.6 mmI.D.
Eluent
0.1N NaOH (pH 13)/acetonitrile (80/20)
Flow rate
0.5 mL/min
Temperature ambient
Time
6 hours
Analytical conditions
Eluent
Flow rate
0.5 mL/min
Temperature 30
Sample
1.Uracil
2.Methyl benzoate
3.Butyl benzoate
10
20 min
10
20 min
Flow conditions
Column
YMC-Pack PolymerC18 1504.6 mmI.D.
Eluent
100 mM H3PO4 (pH 1.7)
Flow rate
0.5 mL/min
Temperature ambient
Time
100 hours
Analytical conditions
Eluent
Flow rate
0.5 mL/min
Temperature 30
Sample
1.Uracil
2.Methyl benzoate
3.Butyl benzoate
54
01.indd 54
11.1.28 10:41:43 AM
Different separation characteristics from silica-based ODS (aromatic compounds)

Polyphenol
YMC-Pack ODS-A
YMC-Pack PolymerC18
1.
3
HO
2.
OH
OH
OH
OH
Phloroglucinol
3.
OH
Resorcinol
Phenol
1
3
10
20
30 min
Column
YMC-Pack PolymerC18 and YMC-Pack ODS-A

1506.0 mmI.D.
Eluent
Temperature 30
5 10 min
Different separation characteristics from silica-based ODS (unsaturated compounds)

YMC-Pack PolymerC18
Triterpene saponins
YMC-Pack ODS-AM
1.
2.
19
ODS
18
1,2
COO--D-Glc
HO
COO--D-Glc
HO
HO
HO
CH2 OH
CH2 OH
0.00
7.50
15.00
22.50 min
0.00
7.50
15.00
22.50
min
Column
YMC-Pack PolymerC18 and YMC-Pack ODS-AM

1504.6 mmI.D.
Eluent
methanol/water (45/55) for PolymerC18

methanol/water (50/50) for ODS-AM
Temperature 50
Analytical columns
Guard cartridge columns (with inner diameter 2.0 mm: 2/pack, 4.0 mm: 3/pack)
Particle size
m
Column size
Product number
S-6
2.0 75
2.0150
4.6150
4.6250
6.0150
6.0250
4.6250
4.6300
6.0250
6.0300
PC99S06-L502WT
PC99S06-1502WT
PC99S06-1546WT
PC99S06-2546WT
PC99S06-1506WT
PC99S06-2506WT
PC99S10-2546WT
PC99S10-3046WT
PC99S10-2506WT
PC99S10-3006WT
S-10
S-6
S-10
10250
PC99S06-2510WT
10250
20300
PC99S10-2510WT
PC99S10-3020WT
Particle size
m
Column size
Product number
S-6
2.010
4.023
PC99S06-0102CC
PC99S06-G304CC
Cartridge holder
Cartridge holder
Guard columns
S-6
S-10
4.010
5.010
1030
4.010
5.010
1030
2050
XPCHSMW
XPCHW
PC99S06-0104WFG
PC99S06-0105WFG
PC99S06-0310WTG
PC99S10-0104WFG
PC99S10-0105WFG
PC99S10-0310WTG
PC99S10-0520WTG
55
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01.indd 56
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YMC GENERAL CATALOG

Types and characteristics of C18

Guides for selecting C18 packing materials

YMC-Pack ODS series

Types and characteristics of C18 packing materials

Types of C18 packing materials

Reversed-Phase C18 Columns

Types and characteristics of C18 (ODS) packing materials

Hydrosphere C18 120

Hydrosphere C18 120

Versatile hybrid silica based

2 m-ODS column for Ultrafast LC method

Processed with YMC Co., Ltd.s

2.0 8.0 Superior separation of

Highly durable ODS

Currently in use worldwide

ODS with wide pore size

Outstanding lot-to-lot reproducibility

For separation utilizing residual silanol

Guides for selecting C18 packing materials

Chemical structures dier

YMC-Pack Pro C18 RS

YMC-Pack ODS-A (Wide-Pore)

Sugars and glycosides

High molecular weight peptides

YMC-Pack ODS-A (Wide-Pore)

Alkaline mobile phase

Reversed-Phase C18 Columns

General organic compounds

Versatile Hybrid Silica Based HPLC Column

Rugged, long lasting, hybrid particle technology

Great chemical durability

Long column lifetime over wide pH range.

Hybrid silica base

Reversed-Phase C18 Columns

organic/inorganic hybrid silica

C18 (as USP-L1)

YMC-Triart C18Integration of triple artistic technologies

YMC-Triart C18Integration of triple artistic technologies

Narrow pore distribution

Hybrid particle for Triart

Silica particle for

Hybrid particle for

Use of microreactor technology produces homogeneous and uniform

YMC-Triart C18 has a very narrow pore size distribution

3Surface modification technology

Reversed-Phase C18 Columns

Reaction image for "multi-stage endcapping"

Evaluation of silanol activity

Endcapping (EC) group

Versatile Hybrid Silica Based HPLC Column

Quality ControlExcellent reproducibility

Low back-pressureFree from pressure problem

Pro C18, ACN/W

Standard ODSBest balance hydrophobicity

Organic solvent rate (%)

YMC-Triart C18 is designed for use in all conditions. Even with

Hydrogen-bonding capacity (caffeine/benzene)

Theoretical plate number

Column pressure (MPa)

Theoretical plate number

Reversed-Phase C18 Columns

CAPCELL PAK C18 AQ

Versatile hybrid silica based

2 m-ODS column for Ultrafast LC method

Processed with YMC Co., Ltd.s

2.0 8.0 Superior separation of

Currently in use worldwide

Outstanding lot-to-lot reproducibility

For separation utilizing residual silanol

A) water/HFBA* (100/0.1)

YMC-Triart C18 (5 m, 120 )

YMC-Triart C18 (5 m, 120 )