Efficacy Testing of Biocidal Products

October 2012
Efficacy testing of biocidal products

overview of available tests
Mall-id: MAG-0006, 2011-12-28
This report was produced by FB Engineering in 2008 at the request of the Swedish
Chemicals Agency.
Kemikalieinspektionen
Postadress
Besk
Faktureringsadress
Telefon & fax
Internet
Org nr
Box 2
172 13 Sundbyberg
Esplanaden 3A,
Sundbyberg
FE 124
838 80 Hacks
Telefon +46 8 519 41 100

Fax +46 8 735 76 98
www.kemi.se
kemi@kemi.se
202100-3880
At the request of
SWEDISH CHEMICALS AGENCY (KEMIKALIEINSPEKTIONEN),
DEPARTMENT OF PESTICIDES AND BIOTECHNICAL PRODUCTS
EFFICACY TESTING
OF
BIOCIDAL PRODUCTS
- Overview of available tests
Gteborg 2008
Ann-Sofie Wernersson (asw@fbe.se)
FB Engineering AB
Skrgrdsgatan 1, Gteborg
Postal address: Box 12076, SE-402 41 Gteborg, Sweden
Phone: +46 31 775 1000
www.fbe.se
Acronym
AATCC
AFNOR
AOAC
ASTM
ATCC
BBA
BP
BPD
BSI
CA
CEB
CEFIC
CEN
CEPE
CSMA
CTBA
EBPF
EPA
EPPO
ISO
MAFF
MS
OECD
OPPTS
PT
SABS
ACRONYMS AND ABBREVIATIONS
Full names
American Association of Textile
Chemists and Colors
Association franaise de
normalisation (NF standards)
Association of Official Analytical
Chemists
American Society of Testing and
Materials
American Type Culture Collection
Federal Biological Research Centre
for Agriculture and Forestry
(Biologische Bundesanstalt fr Land
- Und Forstwirtschaft
Bundesrepublik Deutschland)
Biocidal Product
Biocidal Product Directive (referring
to 98/8/EG)
British Standards Institute (BS
standards)
Competent Authority
Commission Des Essais
Biologiques
European Chemical Industry
Council
European Committee for
Standardisation
European council of paint, printing
inks and artists colours industry
Chemical Specialties Manufactures
Association
Centre Technique du Bois et de
lAmeublement, Bordeaux
European Biocidal Product Forum
United States Environmental
Protection Agency
European and Mediterranean Plant
Protection Organization
International Standards
Organisation
Ministry of Agriculture Fisheries and
Foods
Malaysian Standards
Organisation for Economic Cooperation and Development
Office of Prevention, Pesticides and
Toxic Substances, United States
Environmental Protection Agency
Product Type
South African Bureau of Standards
Web page to organisations (if available)

www.aatcc.org/
/ww.afnor.fr/
www.aoac.org/
www.astm.org/)
www.bba.de
www.bsi.org.uk/
www.afpp.net/commande/commissions/CEB.htm
www.cefic.org
www.cenorm.be
www.cepe.org
www.csma.org
www.ctba.fr
www.epa.gov
www.eppo.org
www.iso.org/iso/home.htm
http://msonline.sirim.my/msonline
www.oecd.org
www.epa.gov/internet/oppts/
www.sabs.co.za
SUMMARY
Efficacy of a biocidal product can be defined as the power to produce an effect,

and is related to the intrinsic efficacy of the active substance/s of the product and
is influenced by the presence of non-active substances and the use pattern. In the
European Community, authorisation of biocidal products is regulated by the
Biocidal Products Directive (98/8/EC). Whereas active substances are assessed at
Community level, the actual products are subject to national authorisation.
Annex IIB of the directive requires likely information for the basis of a label claim
but there is at present no international consensus on specific data requirements
for different product types. For several product types there is no information in the
TNsG on Product Evaluation, on how the tests are performed and whether there
are standards available. In Sweden, information about efficacy was previously not
regarded as important for the approval decision-making in national product
applications. The aim of this project was therefore to provide the Swedish
Chemicals Agency with an overview and brief description of available efficacy test
methods (both standards and others) for all product types and information on how
the tests are evaluated and what efficacy criteria are applied.
Test references and, to some extent, test descriptions were found by searching for
available test procedures and contact persons on web pages, by e-mail and phone
correspondence with authorities, industry organisations, standardization
organizations, individual manufacturers and test/research institutes. The present
overview covers all product types except PT 16, 22 and 23 and includes 387 test
references, of which 341 are standard methods. For one third of the tests, the
scope, design, applicability and/or efficacy criteria is briefly summarised. In
addition, the test references of more than 250 standards have been checked to
make sure that the newest version available is included in the overview and to
exclude tests that have been withdrawn.
The numerous ways of testing and measuring efficacy makes comparisons
between products and the setting of performance standards difficult. Lack of
performance standards and specific data requirements could result in inconsistent
evaluations in different Member States. Therefore, authorities, standardisation
organisations and industry representatives need to develop pass/fail criteria, new
test standards, identify reference products and establish lists of recommended
tests for particular product applications. As a start, the lists of available tests in the
current version of the TNsG on Product Evaluation need to be updated.
SAMMANFATTNING
Effektiviteten hos biocidprodukter kan definieras som frmgan att stadkomma en effekt
och beror av den inneboende effektiviteten hos den aktiva substansen i produkten. Den
pverkas dock ven av andra substanser i produkten och anvndningsmnstret. Inom EG
regleras biocidprodukter av biociddirektivet (98/8/EC). Aktiva substanser bedms p EG
niv medan produkterna regleras nationellt.
I direktivets Annex IIB beskrivs de informationskrav som ligger till grund fr den pstdda
effektiveteten hos produkten. Det rder dock fr nrvarande ingen internationell
samstmmighet nr det gller specifika datakrav fr respektive produkttyp fr att styrka
den pstdda effektiviteten. I TNsG on Product Evaluation saknas information fr flera
produkttyper nr det gller testdesign och huruvida det frekommer ngra standarder. I
Sverige ansgs tidigare att effektivitetsdata inte var viktiga fr tillstndsprvningen av
biocidprodukter. Syftet med freliggande rapport r drfr att frse Kemikalieinspektionen
med en sammanstllning och kort beskrivning (inklusive hur de utvrderas och vilka
effektivitetskriter som rder) av tillgngliga effektivitetstester (bde standarder och vriga),
fr samtliga produkttyper.
Test referenser och till viss del ven testbeskrivningar erhlls genom att ska efter
testmetoder och kontaktpersoner via hemsidor, e-mail och telefonkontakt med
myndigheter, branschorganisationer, standardiseringsorganisationer, tillverkare,
kommersiella laboratorier och forskningsinstitut. Sammanstllningen tcker in alla
produkttyper frutom PT 16, 22 och 23 och innehller referenser till 387 effektivitetstester,
varav 341 r standarder. Fr en tredjedel av testerna summeras eller citeras syfte, design,
applicerbarhet och/eller effektivitetskriteria. Dessutom har mer n 250 testreferenser
kontrollerats fr att skerstlla att den senaste versionen tas med i sammanstllningen
och fr att frhindra att tester som inte lngre r giltiga r med.
Eftersom effektivitet kan underskas och mtas p s mnga olika stt blir det svrt att
jmfra effektiviteten hos olika produkter och att enas om krav p en viss prestanda hos
produkterna inom samma typ och anvndningsomrde. I kombination med att det saknas
specifika testkrav finns det risk fr en inkonsekvent bedmning i olika medlemslnder. Det
r drfr ndvndigt att myndigheter, standardiseringsorganisationer och industrin
tillsammans stter prestandakrav, utecklar nya standardtester, tar fram referensprodukter
samt arbetar fram listor p rekommenderade tester fr specifika produkttyper och
anvndningsomrden. En frsta brjan r att uppdatera de listor ver tillgngliga tester
som finns i den nuvarande versionen av "TNsG on Product Evaluation".
TABLE OF CONTENTS
ACRONYMS AND ABBREVIATIONS ................................................................. 1
SUMMARY .......................................................................................................... 2
SAMMANFATTNING .......................................................................................... 3
BACKGROUND AND AIM OF THE PRESENT OVERVIEW ............................... 6
4.1
BACKGROUND .................................................................................................. 6
4.1.1
Biocidal Products Directive .............................................................................. 6
4.1.2
Data requirements related to efficacy .............................................................. 6
4.2
AIMS OF THIS OVERVIEW ................................................................................ 8
APPROACH ........................................................................................................ 9
INTRODUCTION ............................................................................................... 10
6.1
PERFORMANCE STANDARDS vs LABEL CLAIMS ....................................... 10
6.2
EXPERIMENTAL DESIGN OF EFFICACY TESTS ........................................... 10
6.3
STANDARDISED AND "IN HOUSE" PROCEDURES ...................................... 11
6.3.1
PT 1-5 ............................................................................................................... 11
6.3.2
PT 6: In-can preservatives .............................................................................. 12
6.3.3
PT 8: Wood preservatives ............................................................................... 13
6.3.4
PT 10: Masonry biocides ................................................................................. 14
6.3.5
PT 13: Metalworking fluid preservatives ........................................................ 14
6.3.6
PT 14: Rodenticides ........................................................................................ 14
6.3.7
PT 18: Insecticides, acaricides and products to control other

arthropods ....................................................................................................... 15
6.3.8
PT 21: Antifouling products ............................................................................ 15
RESULTS.......................................................................................................... 15
7.1
TEST REFERENCES FOUND IN THIS OVERVIEW ......................................... 15
7.1.1
International standardisation organisations own searches ...................... 16
7.1.2
Tests used in other MSs TM and national guidelines ................................ 17
7.1.3
Methods in use according to manufacturers and industry

organisations ................................................................................................... 18
7.1.4
Test endpoints ................................................................................................. 19
7.2
LIMITATIONS AND KNOWLEDGE GAPS ........................................................ 19
DISCUSSION .................................................................................................... 20
8.1
EVALUATING EFFICACY COMPARING TEST RESULTS TO

LABEL CLAIMS AND PERFORMANCE STANDARDS ................................... 20
CONCLUSIONS AND SUGGESTIONS............................................................. 22
10
APPENDIX I. TEST PROCEDURES (STANDARDS AND OTHER

REFERENCES). ................................................................................................ 23
BACKGROUND AND AIM OF THE PRESENT OVERVIEW
4.1
BACKGROUND
4.1.1
Biocidal Products Directive
In the European Community, authorisation of Biocidal Products (BP) is regulated

by the Biocidal Products Directive (BPD) 1. In regulating the use of BPs, the
benefits are to be balanced against the risks. The authorisation of a BP should
only be granted if that product is shown to be sufficiently effective (article 5(1)(b) of
BPD) without posing an unacceptable risk to the environment. There should also
be no other unacceptable effects (such as development of resistance).
Efficacy can be defined as the power to produce an effect, and is described in
Annex IIB and VI of the BPD as the ability to fulfil label claims. The efficacy of a
BP determines the lower concentration or dose limit that should be used. Efficacy
is not only related to the intrinsic efficacy of the active substance/s of the product
but also affected by non-active substances (such as surfactants and solvents)
included in the product. The efficacy of a product is also related to the use pattern
of the product. Knowledge about how BPs are used is therefore essential in the
assessment of the product.
Active substances used in BPs placed on the market are assessed at Community
level and must first be listed on Annex I, IA or IB of the BPD. In order to include an
active substance on Annex I, IA or IB, the notifier needs to demonstrate that the
active substance is effective in at least one application.
Products are subject to national authorisation (BPD Annex VI, articles 51, 52, 92,
93). Label claims are a central issue in the assessment of efficacy. The applicant
needs to submit data on the product to substantiate the label claim and intended
uses, and efficacy claims must be presented on the product label and in product
information. Efficacy claims that could cause the product to be used in an
inappropriate way should be avoided, in order to minimize biocide use and
unwanted side effects.
4.1.2
Data requirements related to efficacy
Data requirements are listed in Annex IIA, IIB, IIIA, IIIB as well as IVA and IVB of
the BPD. Product related data is found in part B of these Annexes, whereas data
1
BPD: Directive 98/8/EC of the European parliament and of the council of 16 February 1998
concerning the placing of biocidal products on the market.
on the active substance is found in part A. The data and information required,
relevant to the effectiveness of the BP is found in Annex IIB and the corresponding
section 5 of the Technical Notes for Guidance (TNsG) on Data Requirements 2.
However, there is no international consensus on what data are required to prove
the efficacy of a BP. In the TNsG on Product Evaluation 3, it is stated that there are
no existing guidelines on how to test the efficacy of BPs and no international
agreements on label claims regarding supportive data or quality assurance
aspects of how such data are produced.
4
In an OECD survey from 1999, some questions on how authorities regarded

data on the efficacy of BPs were included. At the time of the survey, not all
countries (including Sweden) had an approval and/or notification process for all
5
Product Types (PTs) , which means that there were also no efficacy data
requirements for these PTs. Also, not all countries regarded efficacy data as
important for the approval process. Ireland did not require any efficacy data at
all, and Sweden did not consider them important for approval decision-making
since they believed that the market will remove products that are not effective
enough.
On the other hand, among the European countries in the survey, Belgium,
Denmark, France, Germany, Greece, Hungary, Netherlands, Portugal,
Switzerland and the UK considered insufficient efficacy as a cause for not
authorising a specific biocide product, at least for some product types. In
Belgium, tests simulating practical use conditions were required for biocides for
non agricultural use. For use in agriculture, efficacy data were needed for
disinfectants for veterinary use and for products against ecto-parasites. In
Denmark, disinfectants were validated on data from a European suspension
test or similar. Nordic standards were used to assess wood preservatives and
paper industry was evaluating effectiveness of slimicides for pulp production. In
Germany, efficacy data were the most important data for approval of vector
control products (insecticides, acaricides and rodenticides in the field of
hygiene) and the actual testing was performed by the Robert-Koch Institute
(disinfectants), and by the Federal Environmental Agency. The testing included
laboratory and field tests, and was based on vector extermination. Although
Finland did not consider insufficient efficacy as a cause for not authorising a
specific biocide product, wood impregnation chemicals were tested and
assessed by the Nordic Wood Preservation Council (non obligatory inspection)
and the Agricultural Research Centre tested the efficacy of all insecticides,
rodenticides and repellents.
Technical Guidance Document in support of the directive 98/8/ec concerning the placing of
biocidal products on the market - guidance on data requirements for active substances and biocidal
products
3
TNsG in support of Annex VI of directive 98/8/EC of the European Parliament and the Council
concerning the placing of biocidal products on the market. Common principles and practical
procedures for the authorisation and registration of products. Ver 10.0, July 2002.
4
OECD Environmental Health and Safety Publications, Series on Pesticides No 9. Report of the
Survey of OECD Member Countries Approaches to the Regulation of Biocides. Paris 1999.
ENV/JM/MONO(99)11. This report (and its annexes) can be found at
http://www.oecd.org/document/18/0,3343,en_2649_32159259_32480722_1_1_1_1,00.html
5
The 23 different Product Types (PTs) are described in Annex V of BPD.
In chapter 7 of the TNsG on Product Evaluation, there is some guidance on how to

proceed. It focuses particularly on information that is needed for stating label
claims, study robustness and report details, Quality Assurance procedures,
evaluation of data and decision making. Any efficacy claims will depend on e.g.
the PT use patterns and desired effects (including target organisms). If the product
has a broad label claim, suitable principal organisms should be identified as the
target organisms.
Annex IIB of the BPD requires likely information for the basis of a label claim. This
includes PT, spectrum of biological activity [target organisms and their
development stage and function as well as mode of action (used also to evaluate
potential and existing pest resistance)], area of use/site of application,
geographical variability, duration of control/effect, directions for use, other relevant
information (including target dose rate, variability and application method), efficacy
or inefficacy of product under certain conditions (nature of infestation, density of
microorganisms, application temperature).
4.2
AIMS OF THIS OVERVIEW
The guidance provided in the TNsG on Product Evaluation is not in the form of a
checklist and there are no rigid criteria to evaluate efficacy data. Expert scientific
judgement is therefore necessary on a case by case basis. As stated in section
7.1.2. of the TNsG on Product Evaluation, the lack of harmonisation of efficacy
requirements can result in uncertainty, confusion, inconsistency and
misunderstandings regarding the extent of efficacy data required by the regulatory
authorities.
The TNsG on Product Evaluation includes references to available standard tests
for Product Type (PT) 1-6, 8, 10, 13-15, 18 and 21. Nevertheless, for several PTs
there is no information on how the tests are performed and whether there are
standards available (PT 7, 9, 11, 12, 16, 17, 19, 20, 22, 23).
In Sweden, information about efficacy was not regarded as important for the
approval decision-making in national BP applications (dossiers). Moreover, some
of the PTs that are now regulated by the BPD were previously exempted from
regulation (including food and feed area disinfectants, in-can preservatives and
metal working fluid preservatives) and some regulated PTs were not subject to an
authorisation process (human and veterinary hygiene biocide products,
preservatives for food and feedstocks, embalming and taxidermist fluids and
biocides for other vertebrates).
In order to be better prepared for evaluating the efficacy data of BPs according to
the BPD, the aims of this project were to provide the Swedish Chemicals Agency
(Kemikalieinspektionen, KemI), i.e. CA Sweden with
an overview and brief description of available efficacy test methods
(standards and "in house") for BPs, including all PTs
information on how the tests are evaluated and what efficacy criteria are
applied.
Knowledge gaps should also be identified.
APPROACH
The present overview includes references to both standards and non standard ("in
house") tests submitted by individual companies. Test references and, to some
extent, test descriptions were found by searching for available test procedures and
contact persons on web pages, e-mail and phone correspondence with authorities,
industry organisations, standardization organizations, individual manufacturers
and test institutes. Some material (including protocols from a Technical Meeting in
2005 at ECB) were also provided by KemI (CA Sweden).
Much effort was made to contact individual persons in companies and test
institutes, either by e-mail and/or phone, in order to include also test references to
"in house" tests and information on what standards that are actually used by
individual companies.
The web pages of the following standardisation organisations were searched for
efficacy tests currently available: ASTM, CEN, EPPO, ISO and OECD. Search
phrases included "efficacy", "antiseptics", "disinfectants", "antifouling",
"bactericides", "preservatives". It was also valuable to search standards through
the Technical Committees and Working Groups, such as E35 for Pesticides under
ASTM.
In order to obtain a starting point for available efficacy tests, the TNsG on Product
Evaluation and national guidelines (UK and NL) were also consulted.
The project was performed during the period of November-December in 2007.
INTRODUCTION
6.1
PERFORMANCE STANDARDS vs LABEL CLAIMS
Performance standards refer to a predetermined efficacy required by the

authorities and should, according to the TNsG on Product Evaluation, be the same
within all Member States for a particular use and situation. The performance
standard ("pass/fail criteria") can be expressed in quantitative or qualitative 6 terms.
Label claims are made by the manufacturer and written on the product label. It
makes a claim about the function of the product, such as pest organisms that it
controls, duration of control, type of control (kill, repel etc), and directions for use
(including method and application rate).
6.2
EXPERIMENTAL DESIGN OF EFFICACY TESTS
The experimental design of efficacy tests can be divided into three different types:
screening tests, simulation tests and field tests.
Screening tests are usually laboratory studies of either the active substance or
simple formulations and often performed during a relatively short period of time.
Studies may include dose-response tests and several replicates. Laboratory data
are usually not sufficient to predict actual treatment levels or effectiveness in
service under real situations, but can be used to estimate the innate efficacy of the
product. An untreated control should be included.
Simulation tests are performed in artificial environments that resemble the real
conditions during use and often the actual product is used in the test. An untreated
control should be included.
Field studies generate data based on actual use of the product as prescribed by
the product label. Even if the exposure conditions are more realistic than the
screening tests it should be kept in mind that they are still only representing a
particular situation and efficacy could be influenced if changing e.g. the level of
pest pressure or application technology. In addition, even if time scales are
generally longer, it is frequently not possible to perform the test during the full life
cycle of the product. PT 8 tests last e.g. 5 years but the service life might be 60
years.
including e.g. percentage kill, extent of remaining population, greatest dilution of product still
producing the desired effect etc
10
The product should preferably be tested at a variety of application rates, including

levels below those suggested in a real use situation. The last point ensures that
the biocidal product dose will be as low as possible.
As in any laboratory testing of chemicals, and also stated in the TNsG on Product
Evaluation, it is appropriate to include a negative (untreated) and/or positive (a
product/test material with the same use pattern and with proven efficacy, often
called a reference product) control. The negative control provides important
information to estimate the extent of the problem without any treatment. However,
for field tests, there are often limited possibilities to include replicates in the study
and to include untreated areas as a control. The latter aspect is sometimes
approached by including pre- and posttreatment assessments. Standard BPs, to
be used as positive controls in the efficacy tests, have been developed for e.g. PT
18 and 8 and can facilitate the ranking of products but also help to control the
reproducibility of a test and facilitates comparisons with other test facilities.
6.3
STANDARDISED AND "IN HOUSE" PROCEDURES
As stated in BPD Annex VI, efficacy testing should be carried out according to
Community guidelines if available and applicable, but other methods may also be
used, including other international standards (e g CEN, ISO), national standards,
industry standards, individual producer standards or data from product
development, as long as these are accepted by the Competent Authority (CA).
The availability of international standards varies between PTs. In the TNsG on
Product Evaluation, there are several standards listed for PT [1-5] 7, [8] 8, [14] 9 and
[18] 10; and a few for PT [6] 11, [10] 12, [13] 13, [15] 14 and [21] 15. For PT 1-5, 6, 8, 10,
13, 14, 18 and 21 some of the information and guidance provided in the TNsG on
Product Evaluation is summarised below. For further details, the reader should
consult the original reference.
6.3.1
PT 1-5
Typical use patterns: health care area for hard surfaces (including medical
equipment), public area for reduction of nuisance, pathogens or algae to
acceptable level, for veterinary or other animal accommodation areas to
prevent/control outbreak of diseases, as bacteriostats against
from CEN, AOAC/EPA, ASTM and MAFF

all from CEN; in particular, EN599 describes pass/fail criteria
9
from EPA/OPP, BBA, EPPO and ASTM
10
from a number of sources, including BS, US CSMA Aerosol guide, AFNOR, WHO, South African
Bureau of standards, EPPO, ASTM and AATCC
11
from AFNOR, DIN, SABS, ASTM
12
from BS and ENV
13
from ASTM
14
from ASTM and EPA
15
from CEPE and ASTM
8
11
6.3.2
microorganisms causing aesthetic problems (odour) in presence of

moisture.
The EN approach is to divide the tests into phase 1, phase 2 and phase 3
tests, corresponding to testing of innate activity, simulated use and "field"
testing (under practical conditions) respectively. Phase 2 tests are
subdivided into 2 steps (suspension tests and others):
o Phase 1: Suspension tests to test innate bactericidal, fungicidal
and/or sporicidal activity; defining minimum standards.
o Phase 2, step 1: Suspension tests, simulating the conditions during
practical use
o Phase 2, step 2: Other tests (handwash, handrub, surface test),
simulating the conditions during practical use
o Phase 3: Field tests (practical conditions)
Broad label claims for PT 2, if intended to be used as a disinfectant in public
hygiene, should indicate efficacy against Gram negative and Gram positive
bacteria. Furthermore, if intended to destroy tuberculosis bacteria, claims
against Mycobacterium tuberculosis will need to be substantiated.
Influencing factors to consider during efficacy testing of disinfectant
biocides include water hardness, interfering substances, contaminants,
temperature, contact time, pH and the roughness of the surface.
PT 6: In-can preservatives
Spoilage of in-can and in-tank products may be the result of

microorganisms (primarily bacteria 16 but also yeast 17 and moulds18)
feeding on substances present in the product, leading to reduced integrity.
By-products of microbial growth can also contribute to spoilage and
consequences include discoloration, gassing, viscocity loss etc.
Laboratory tests are performed either as MIC (Minimal Inhibitory
Concentration), challenge testing or heat stability testing. The usual method
for evaluating in-can preservatives in paints or other aqueous products is
the challenge test.
o MIC determinations are conducted on a dilution series of the active
substance. The minimum amount of biocide that is required to inhibit
microbial growth is identified and efficacy is assessed against a
range of bacterial, fungal and yeast spoilage organisms. Results are
presented as the concentration required to inhibit the growth of a
particular test organism and are useful for determining the spectrum
of activity of an active substance.
o In the challenge test, microbial cells are added to the test sample
and the survival or death rate of these cells is monitored with respect
to time.
16
Common species are Alcagenes spp, Micrococcus luteus, E. coli, Proteus vulgaris.
Common species are Aspergillus spp, Geotrichium candidum, Penicillum spp.
18
Common species are Candida albicans, Rhodotorula rubra, Saccharomyces cerevisiae
17
12
6.3.3
o The level of active substance is usually measured by a suitable

chromatographic method at time zero and after incubation at an
elevated temperature to determine whether the biocide has
degraded. Incubation time and temperature varies between tests and
the results indicate the stability of the active substance in a particular
product formulation.
US EPA assessment criteria require that the active substances should
show effectiveness against bacteria in at least two representative
formulations in which the biocide is intended for use and tested (simulateduse) in at least three replicates of each of the two product formulations.
Actual bacterial isolates (identified at least to genus) from spoiled product
and/or ATCC (American Type Culture Collection) spoilage bacteria should
be employed as test inocula. Mixed bacterial and fungal inocula are not
acceptable in demonstrating bacterial deterioration. Bacteriological
sampling should be quantitative and product quality observed concurrently.
Test duration: 6 m to 1 yr. Conditions in the negative controls should cause
significant growth and deteriorative (physical and chemical) changes.
PT 8: Wood preservatives
Durability of treated products will rely on the residue in wood which remains
active for the claimed service life. Efficacy will to a large extent depend on
climate (extremely variable situations exist within the European
Community), target organisms, wood species, application process19 and
exposure class (see below).
Label claims should include a statement whether the product is aimed at
fungal, insect or attack from marine borers, and whether it is a preventive or
remedial (curative, eradicant)20 product. The test data required for remedial
claims may need to consider both preventive and eradicant action.
The data required will depend on the Use/Hazard classes (see e.g. EN 3351, -2, -3), based on increasing level of wetting degree and exposure
severity: Hazard class 1: Above ground (Dry) exposure; class 2: Above
ground (wetting, protected from the weather); class 3: Above ground
(Exposed to weathering, but not in ground contact); class 4: Timbers in
contact with the ground or fresh water, or above ground if water trapping or
logging exist. Hazard class 5: Timbers in the marine environment 21.
19
There are three types of application methods: Penetrating treatments (including double
vacuum, vacuum-pressure and diffusion), surface treatments (including brush and spray
techniques and dipping processes) and other treatment methods.
20
The minimum performance requirements for eradicant BPs are given in prEN 14128:2001.
Remedial (in situ) treatment systems will be more varied than preventive treatments, depending on
e.g. a variety of likely treatment methods.
21
Data required to support claims for preventive efficacy, for Hazard class 1: Laboratory test on
wood boring insects (no fungal hazard); for class 2: Laboratory test on Wood rotting
basidiomycetes - brown rot; for class 3: Laboratory test on wood rotting basidiomycetes - brown rot
and in some cases white rot, and an optional field L-joint test (5 year); for class 4: Laboratory test
on Wood rotting basidiomycetes - white rot and brown rot, Laboratory test on soft rot microfungi,
and an optional field Stake test (5 year); for class 5: Laboratory test on wood rotting
basidiomycetes - white rot and brown rot, lab test on soft rot microfungi, lab test on Marine borers,
and a compulsory field Marine test (5 year).
13
6.3.4
PT 10: Masonry biocides
Effectiveness (often described as kill or prevention of regrowth) depends on

substrate, target organisms (including dry rot fungus, fungus/yeasts,
lichens, mosses and liverworts), penetration, concentration used etc.
In laboratory tests for general surface BP use, different concentrations of
the active substance is absorbed on assay discs applied to agar that is
seeded with fungal or algae. The zone of growth inhibition is determined
after incubation. There are also simulated use and field tests.
To test products intended for the control of dry rot, simulated use tests are
based on employing mortar treated with the product and then exposed to
challenge by Serpula lacrymans. Growth over or through the treated
substrate is evaluated. Outbreaks of dry rot are unique and to control growth it is
neccary to implement a number of measures. It is therefore difficult to include
"untreated controls" and to design field tests.
6.3.5
6.3.6
PT 13: Metalworking fluid preservatives
Simple laboratory tests generally determine MIC (Minimum Inhibitory

Concentrations). Many plant variables (such as machine characteristics,
metal, and the present microorganisms - both bacteria and fungi and yeast)
can influence overall preservative efficacy. Field use levels are therefore
often difficult to predict based on laboratory tests.
In simulated use tests the fluid and preservative is generally placed in a
vessel and tested in a shaker, aeration or pump system, regulated in a
manner that simulates the plant conditions. Microbial inoculation is
performed repeatedly and the fluid is monitored regarding visual
appearance, pH, oxygen uptake and the presence/survival of
microorganisms.
Field (in-use) tests will only be applicable to the particular conditions during
the test and it may be difficult to run concurrent negative controls.
PT 14: Rodenticides
Rodent species can cause montory loss (through consumption or spoilage

of foodstuff or damage due to gnawing and burrowing) and spread disease.
Major target organisms include rats and house mice. Selected test species
should be relevant to the geographic region and specified on the label.
Laboratory test methods for products include no choice tests (test
organisms are only offered contaminated food) and choice tests (both
contaminated and uncontaminated food is offered) as well as studies
related to specific product types (contact rodenticides or gassing agents) or
related to specific efficacy claims (for use in damp situations).
14
6.3.7
6.3.8
In field trials /under seminatural conditions/ rodent activity on the site should
be determined before and after treatments and amount of bait consumed
should be monitored.
PT 18: Insecticides, acaricides and products to control other arthropods
The standards can be used for testing efficacy against flying, crawling,
larval insects and also to test fumigants.
Product applications include spray, textiles and fumigation. The effects
include knockdown, kill, residual22, flushing, ovicidal/larvicidal and the
control of resistant pests. Treatment methods include general surface
treatment, crack/crevice treatment, contact (direct) space spray, spot
treatment and baits.
The tests are performed either as lab/screening tests, simulated use tests
or field tests.
PT 21: Antifouling products
Although there are a few standards related to antifouling products, these

are usually related to leaching rate rather than efficacy. The two standards
included produce simulated field data (raft testing) during which panels
coated with the test formulation are immersed in water under static
conditions. Other test principles could be in vitro (lab test) and field (patch)
test.
RESULTS
7.1
TEST REFERENCES FOUND IN THIS OVERVIEW
National and international standards, in house tests and other test references are
included in the list presented in Appendix I, comprising in total 387 test references.
Of these, 341 are standards and 46 are "in house" and other non standardised
procedures developed either by the manufacturers or external test institutes and
research laboratories.
The standard test references already listed in TNsG of Product Evaluation are all
included in the list, except tests that are not considered "efficacy tests" (see below)
and tests that have since been withdrawn. In addition, web pages of some of the
standardisation organisations were searched for the latest versions and currently
22
Residual effects are due to active substances that remain active for a long time (weeks to
months) in order to control e.g. walking insects (that are unlikely to react to space sprays).
Treatments are applied at high dosages to surfaces or as palatable baits.
15
available tests (see next section). Manufacturers, industry organisations, other

MSs and test institutes and laboratories were also contacted regarding tests that
they use or know are being used.
About 90 manufacturers, test institutes and industry organisations were contacted.
Out of these, 37 responded. In some cases there was a high interest in the project
but due to the limited timeline of the project and/or other restrictions some
companies could not contribute with specific test references to particular products
to be included in the overview. In addition, some of the contacted companies did
not manufacture and/or test /the efficacy of/ BPs. Nevertheless, 14 companies
contributed with a total of 130 test references (including both standards and other
test methods) to the overview, and one manufacturer will send references
separately at a later stage.
7.1.1
International standardisation organisations own searches
The web pages of the following standardisation organisations were also searched
for efficacy tests currently available: ASTM, CEN, EPPO, ISO and OECD. The
results are included in Appendix I.
Some additional CEN standards only indirectly related to actual efficacy testing
include (but is probably not limited to) EN 1014 part 1-4 (methods of sampling and
analysis of creosote), EN212:2003 (General guidance on sampling and
preparation for analysis of wood preservatives and treated timber), EN 335-1 and 2 s well as EN 599-1 and -2 and EN 14128:2003 (related to use and hazard
classes, performance criteria and labelling regarding durability of wood and woodbased products). These tests are not included in Appendix I.
EPPO is primarily focused on Plant Protection Products, although some standards
can also be applicable to some biocidal product types, such as rodenticides (PT
14) and fumigants (PT 18). Except for the standards included in Appendix I, they
have also published a Good Plant Protection Practice for rodent control for crop
protections and on farms 23.
ASTM has several standards applicable to microorganisms and wood
preservatives but also other PTs. Most ASTM standards have not been ring
tested, except some of the standards that correspond to USEPA methods.
Working groups searched are E35 (Pesticides, including subcommittees 35.12
Insect control agents, 35.22 Pesticide formulations and delivery systems, 35.15
Antimicrobial agents), E47.01 (Aquatic Assessment and Toxicology), D31.02
(Leather, subcommittee wet blue), D01.28 (Biodeterioration) and D01.45 (Marine
Coatings), both subcommittees under D01 (Paint and related coatings, materials
23
PP 2/5(1) Rodent control for crop protection and on farms. OEPP/EPPO Bulletin 25, 709-736. It
was edited as an EPPO standard in 1998 and can be found at
http://archives.eppo.org/EPPOStandards/gpp.htm
16
and applications), G03.04 (Weathering and durability, biological deterioration),

D14.30 (Wood adhesives, subcommittee under D13 Adhesives).
Some additional ASTM standards only indirectly related to actual efficacy testing
include (but is not limited to): ASTM E1326-06 (Standard guide for evalutating
nonconventional microbiological tests used for enumerating bacteria), ASTM
D5259-92(2006) (Standard Test Method for Isolation and Enumeration of
Enterococci from Water by the Membrane Filter Procedure), ASTM E1427-00e1
(Standard Guide for Selecting Test Methods to Determine the Effectiveness of
Antimicrobial Agents and Other Chemicals for the Prevention, Inactivation and
Removal of Biofilm), D283-84(1990)e1 and D284-88(1993)e1 (related to chemical
analysis of active substances in antifouling products) and D5108-90 (Standard
Test Method for Organitin Release Rates of Antifouling Coating Systems in Sea
Water). These are not included in the list.
ISO standards have been ring tested but there are only a few available published
efficacy standards, included in Appendix I.
As opposed to e.g. fate and ecotoxicity tests, efficacy testing is covered only to a
little extent by OECD guidelines. There are only a few applicable OECD guidelines
published, although there are some drafts, see Appendix I. Five new test methods
to evaluate efficacy of antimicrobials on hard surfaces will be investigated in ring
trial. For the particular case of testing piscicides (PT17), it is possible to use test
guidelines that were developed for aquatic toxicity testing.
The US EPA Office of Prevention, Pesticides and Toxic Substances (OPPTS) has
published updated test guidelines in ten series, of which 810 is related to product
performance 24. Nine guidelines related to Product Performance were finalized and
published on the web page at the time of the present project and included in the
overview.
7.1.2
Tests used in other MSs TM and national guidelines
In the second Technical Meeting at ECB in 2005 (TMII05), the MSs were asked to
submit references to any efficacy tests that they knew were being used within their
territory, based on a list provided by COM. CA France provided a detailed
response and test references on their list have been included in Appendix I, if also
found on the homepages of the standardisation organisations. In the German list,
some standards that are not actually efficacy tests were indicated and therefore
erased from Appendix I.
In the UK, detailed national guidance documents can be found at the Health and
Safety Executive (HSE) website 25 for wood preservatives, rodenticides, anti-fouling
products, surface biocides, as well as insecticides and acaricides. These
24
http://www.epa.gov/opptsfrs/home/guidelin.htm
25 http://www.hse.gov.uk/pesticides/application/index.htm
17
guidelines outline the nature and extent of testing required to gain approval and all
but the antifouling guideline include lists of available standards. Upon contacting
the HSE, it was found that the most commonly used efficacy standards they have
encountered so far are EN standards, but ASTM standards are probably the next
most frequently submitted. They state that industry tests are also common,
especially for some PTs. Wood preservatives have generally been tested
according to CEN standards; insecticides according to in house tests (but where
guidelines are used, they are most frequently WHO tests); rodenticides according
to non standard tests or EPPO guidelines; anti-fouling paints were normally tested
in industry raft tests or patch tests and finally surface biocides were most
commonly tested according to non standard test methods. A list of references to
tests for some PTs that was so far included in the authorisation process was also
provided and these references have been included in Appendix I.
In Denmark, wood preservatives are efficacy tested according to CEN standards.
In the Netherlands, The Board for the Authorisation of Plant Protection Products
and Biocides (Ctgb) 26 is responsible for the authorisation of biocides. On its
website, efficacy concept guidance documents are available for disinfectants,
wood preservatives and rodenticides. There is also a guidance document coming
up on insecticides and a new version of the rodenticide guideline is presently
being negotioated in Technical Meetings. References found in the last version of
the rodenticide guidance document as well as the guidance for wood preservatives
were included in Appendix I.
7.1.3
Methods in use according to manufacturers and industry organisations
In the TNsG on Product Evaluation as as well as national guidelines (UK and NL)
there are several lists of available standard tests available for efficacy testing of
biocide products (see above). However, they do not make any comments on the
suitability of particular test methods and how frequently they are used. In order to
obtain an indication as to what tests are actually applied on the BPs by different
companies today (i.e. what test data the CA Sweden and other Member States
may expect to receive in applications), manufacturers were also contacted to ask
about what tests they use on their products. In addition, several tests available are
not in the form of standards but rather in house methods, sometimes developed
by the companies themselves and therefore not listed in the TNsG on Product
Evaluation.
Companies were initially contacted in Sweden but because suitable contact
persons were usually found to be located abroad, the companies were later mainly
contacted internationally. In addition, test institutes were also contacted, regarding
available efficacy tests on BPs and these references are also included in Appendix
I.
26
http://www.ctb.agro.nl
18
7.1.4
Test endpoints
In the TNsG on Product Evaluation it is pointed out that Good Laboratory Practice
(GLP) is not required for efficacy tests, although the spirit of these principles
should be applied at least during documentation. Due to limited access to full text
test descriptions and the short time period of the project, an in-depth evaluation of
any particular tests could not be performed. The individual design, applicability and
efficacy criteria, was therefore only briefly cited or summarised in Appendix I, if
information was available from either the home page of the standardisation
organisations or from full text references. However, it can be concluded that the
results of efficacy tests are expressed in numerous ways, depending on PT, test
design etc. Some examples of efficacy endpoints are "mean time to 50%
knockdown of flies", % kill, EC50 ("concentration causing the measured effect in
50% of the test population"), "elapsed time to first bite", % ants crossing a tile
within 60 minutes, MIC (Minimum Inhibitory Concentration), % reduction in
CFU (Colony Forming Units) etc, see Appendix I for particular tests.
7.2
LIMITATIONS AND KNOWLEDGE GAPS
In this project, a priority was to cover most or preferably all PTs rather than obtain
in depth information on tests for a particular PT. Although for some PTs only a few
test references were found, Appendix I covers all PTs except PT 16, 22 and 23.
One reason for the major lack of information on some PTs may be that there are
only a few products that will belong to this group (particularly true for e.g. PT 20,
because these products are generally covered by other regulations), but also that
not all product types have been previously regulated in all Member States.
Almost 90 % of the tests included in the overview are standards, although the
situation can vary between different PTs. There are most likely several additional
"in house" and non standardised tests, not available to the author during the
relatively short time period of the project. The coverage related to non standard
tests is related to the contributions obtained from different manufacturers. There
are also several additional standards available, but in this project in-depth
searches were focused on international standardisation organisations rather than
national standards.
19
DISCUSSION
BPD Annex VI, paragraph 92 and 93:

92. Member States shall not authorise a biocidal product which does not
possess acceptable efficacy when used in accordance with the conditions
specified on the proposed label or with other conditions of authorisation.
93. The level, consistency and duration of protection, control or other intended
effects must, as a minimum, be similar to those resulting from suitable reference
products, where such products exist, or to other means of control. Where no
reference products exist, the biocidal product must give a defined level of
protection or control in the areas of proposed use. Conclusions as to the
performance of the biocidal product must be valid for all areas of proposed use
and for all areas in the Member State except where the proposed label
prescribes that the biocidal product is intended for use in specific
circumstances. Member States shall evaluate dose response data generated in
trials (which must include an untreated control) involving dose rates lower than
the recommended rate, in order to assess if the recommended dose is the
minimum necessary to achieve the desired effect.
8.1
EVALUATING EFFICACY COMPARING TEST RESULTS TO LABEL

CLAIMS AND PERFORMANCE STANDARDS
The purpose of testing BPs using efficacy tests, is to determine whether the
products posess acceptable efficacy (see BPD Annex VI, para 92). Statistically
significant results in a quantitative test including a negative control indicates
whether the product is efficient compared to no treatment at all. However, as with
any laboratory testing, whether a particular result is statistically significant is
influenced by aspects such as the number of replicates tested and other factors
related to test design (incubation period, choice of test organism etc). Therefore, a
product may be "efficient" according to one particular test, but maybe not if
applying other test conditions.
Furthermore, statistically significant may not be efficient enough from a users
point of view. A product causing 20% reduction in the number of cockroaches is
probably not regarded as efficient enough. BPs need to be efficient also in real
life and should fulfil minimum requirements for a certain use. Therefore, test
results need to be compared to performance standards (pass/fail criteria).
Individual efficacy test methods usually do not include pass/fail criteria, although
there exists CEN standards with pass-fail criteria for PT 8 (EN599) and several
applications under PT 1-5 (prEN14885:2006). Therefore, in most cases, the
efficacy of BPs has to be evaluated by comparing test results to the label claims
made by the manufacturer. The evaluating authorities need good knowledge about
20
how the BP is used and how it was tested, and the label claim need to be detailed
enough to allow this evaluation.
There are so far very few test recommendations or data requirements (specifying
recommended tests) for efficacy testing of BPs. The meaning of certain definitions
used in the labels, such as "disinfectant" and germicidal effect also need to be
defined on an international level (today different countries use somewhat different
definitions). In addition, standards are not available or there are very few for
several applications, which means that the authorities in these cases need to
evaluate data from manufacturers that have used internal test protocols. This may
cause the need to evaluate not only the test data but also the test protocols used
to generate the data by different manufacturers.
One problem with this case by case approach is that it will be difficult for
authorities to rank different products within a particular PT. Efficacy tests are
performed, and results expressed, in numerous ways, making comparisons
impossible. In addition, the evaluation can become inconsistent between different
CAs.
The lists currently available in the TNsG on Product Evaluation do not make any
statements about the suitability of particular tests, only availability. To fully
harmonise all testing of BPs for a particular PT would be very difficult, due to the
large variety of BPs and use areas, application rates, time scales of the protective
effects etc. However, it would probably be beneficial for both the manufacturers
and the CAs if recommended standard tests or data requirements are available for
all PTs and the most common areas of use. Individual deviations from test
standards can then be allowed if necessary due to the specific use (and label
claim) of the product. These minor deviations in the test design might influence the
results, but difficulties in comparing the results can be partly overcome by using
positive controls.
In general, ranking of different products (under specific conditions) would be
facilitated by the use of standard BPs as positive controls in the test. A postive
control also helps controlling the reproducibility of a test and facilitates
comparisons with other test facilities. When developing these standard BPs, not
only efficacy of the product should be evaluated, but also negative side effects on
non target species. It may not be necessary for the positive control to be the most
efficient product available.
Performance standards should be developed in parallel to lists of recommended
tests and reference products, so that it is indeed possible to determine whether a
particular product fulfils the criteria by testing it according to a certain test. The
quality of the particular test protocols (robustness) needs to be evaluated before
inclusion in the list of recommended/required tests. Authorities, standardisation
organisations, industry associations and manufacturers should all be involved
during the development of pass/fail criteria, new test standards, reference
products identification and establishing listings of recommended tests for particular
product applications.
21
Even with long term field tests it is not easy to predict real performance of the
product. The problems associated with efficacy testing are not unique though;
predicting ecosystem effects of chemicals based on a few toxicity tests performed
in the laboratory is also not a simple task. However, if harmonising performance
standards in so far as possible for particular PTs, use situations and label claims,
the authorisation process as well as the choice of test method and design will be
facilitated.
CONCLUSIONS AND SUGGESTIONS
In total, 387 test references are included in this overview, covering all PTs
except 16, 22 and 23. The overview is based on references from the TNsG
on Product Evaluation and information from manufacturers, MSs and
national guidelines, test institutes and own searches of tests available on
the homepage of some international standardisation organisations. About
90% of the available tests included are standards.
For one third of the test references included in this overview, the scope,
design, applicability and/or efficacy criteria is briefly summarised as far as
possible if information was available.
The test references of more than 250 standards have been checked to
make sure that the newest version available is included in the overview.
Efforts should be made to develop

o performance standards (pass/fail criteria) to minimize inconsistent
evaluation of efficacy
o additional standard methods in order to cover the most frequent
applications
o suitable reference substances, to facilitate ranking of different BPs
that were tested using different procedures and also the evaluation
of the reproducibility of test results.
o lists of recommended tests for particular products and use situations.
Authorities, standardisation organisations and industry representatives

need to be involved during the development of pass/fail criteria, new test
standards, reference products identification and to establish lists of
recommended tests for particular product applications. As a start, the lists
of available standards for efficacy testing of BPs need to be updated in the
TNsG on Product Evaluation, since several tests have been withdrawn or
replaced by newer versions. It is the hope of the author that the present
overview may be of assistance in this process.
22
10
APPENDIX I. TEST PROCEDURES (STANDARDS AND OTHER

REFERENCES).
The following table includes relevant standards from TNsG on Product Evaluation,
list of standards used in France, national guidelines (UK, NL), results from own
searches on the web page of standardisation organisations, standards and non
standardized test refered to and/or supplied by manufacturers, industry
organizations and performing laboratories. The standards related to multiple
sources are updated to the most recent version if applicable. References in italics
have been checked by searching the web page of the standardisation organisation
(reference number, title) and, if applicable, been updated to the most recent
version of the test.
The suitability of particular test methods have not been evaluated, but for several
tests (if information was available to the author), the test procedures and
evaluation criteria have been briefly summarised, based or cited mainly from
information found in the description of the tests available on the web pages of the
standardisation organisations, full test description and/or from the TNsG on
Product Evaluation (TNsG PE).
For ASTM methods, the number after the hyphen is refering to the last revision
date of the test; the number in parenthesis is referring to the last reapproval of the
test.
The table includes only standards that are related to efficacy testing (as far as this
has been possible to read out from the title and available descriptions of the
standard), not e.g. use class standards (for PT8), leaching rate standards (for PT
21) etc. Standards that may have been present in the TNsG on Product Evaluation
but withdrawn since then (such as several ASTM standards related to avicides,
PT15) are also not included. Section 810.2000 of the OPPTS of US EPA is related
to antimicrobials in public health, but none of the guidelines were found on the
EPA web page at the time of the project. Although several EPA guidelines related
to PT 1-5 were included in the TNsG on Product Evaluation, these are not
included in table 1 due to uncertain reference details (titles are missing in the
TNsG PE).
The list is not exhaustive and the PT designation of a particular test reference may
not always reflect possible applications of the test method and in some cases it
was difficult to assign the test to a particular PT.
23
Table 1. Overview of efficacy test references, in order of product type.

REFERE
NCE
TITLE
PT
ASTM
E189197(2002)
Standard Guide for

Determination of a
Survival Curve for
Antimicrobial Agents
Against Selected
Microorganisms and
Calculation of a DValue and
Concentration
Coefficient
Test Method for
Determining the TimeKill Kinetics Of
Antimicrobial
Compounds
Standard Practice for

Evaluating Microbial
Susceptibility of
Nonmetallic Materials
by Laboratory Soil
Burial
810?
ASTM
WK15324
ASTM
G160-03
SHORT TEST DESCRIPTION

(IF TEST METHOD
AVAILABLE OR
INFORMATION PROVIDED
FROM ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
Own searches
This test method assesses the

microbicidal activity of
antimicrobial materials,
whereby the survival of
organisms exposed to a water
miscible antimicrobial agent is
determined as a function of
time. The primary purpose of
this method is to provide a set
of standardized conditions and
test organisms to facilitate
comparative assessments of
antimicrobial materials
miscible in aqueous systems.
A wide variety of properties
may be affected by microbial
attack depending on material
or item characteristic.
Standard methods (where
available) should be used for
each different property to be
evaluated. This practice does
not attempt to enumerate all of
the possible properties of
interest nor specify the most
appropriate test for those
properties. Test methods must,
however, be appropriate to the
material being tested.
Evaluation of a nonmetallic
material's microbiological
susceptibility when in contact
with the natural environment of
the soil and is intended for use
on material test specimens
that are approximately 2 cm
(3/4 in.) thick and 100 cm2 (20
in2) or less. It is recommended
that this practice be combined
24
Own searches
Own searches
REFERE
NCE
TITLE
PT
Alleman,
J.E.,
Etzel,
J.E.,
Gendron,
D.,
Kirsch,
E.J.,
Conley,
J.,
Fidelle,
T.,
Handy,
F., and
Hildebran
dt, M.
nd
42
Purdue
Industrial
Waste
Conferen
ce, May
1987,
519-524
American
Public
Health
Associati
on, 16th
Edition,
1985
Comparative
Evaluation of
Alternative Halogenbased Disinfection
Strategies
1-5
Standard Methods for

the Examination of
Water and Wastewater
(SMEWW).
1-5

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
with appropriate environmental
exposures (for example,
sunlight simulating weathering
devices, the hydrolytic effects
of extended aqueous contact,
or extraneous nutrients) or
fabrication into articles (for
example, adhesive bonding of
seams) which may promote
microbiological susceptibility
during the service life of
material.
Microbiological susceptibility
may be reflected by a number
of changes including staining,
weight loss, or reduction in
tensile or flexural strength.
This practice may be applied
to articles that do not spend
the majority of their service life
in soil.
Microorganisms were exposed
to a mixture of biocide in
solution for 4 or 16 minutes.
The percentage survival was
assessed under various
conditions such as high or low
nitrogen (as ammonium), high
pH or low temperatures.
TYPE OF
REFERENCE
SOURCE
A swimming pool trial was

performed over a three month
period to demonstrate the
disinfection efficacy of the
product. Water samples were
collected for microbial
analysis. Water and, air
Manufacturer
25
Manufacturer
REFERE
NCE
TITLE
PT
AOAC
(Associati
on of
Official
Analytical
Chemists
) official
method
965.13
ASTM
E105296(2002)
Disinfectant (water)
for Swimming Pools
official method of
Analysis, 16th Edition,
1995
1-5
Standard Test Method

for Efficacy of
Against Viruses in
Suspension
1-5

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
temperature, number of
bathers (at the time when
samples were taken and total
bathers for the day), eye and
skin irritation and water clarity
were also measured.
Criteria for effective control
was determined as follows:
The state of California,
Department of Health Services
requirement for pool water is;
Bacteriological quality of water
in the swimming pool shall be
such that not more than two
consecutive samples, taken
when the pool is in use shall:
1. Contain more than 200
bacteria per ml, as determined
by the standard plate count; or
2. Contain a total coliform
organism count of 2.2 or
greater per 100 ml of sample
3. Chemical quality of water in
the pool shall not cause
irritation of eyes or skin of the
bathers, or have other
objectionable physiological
effects on bathers.
TYPE OF
REFERENCE
SOURCE
Manufacturer
Laboratory suspension test

that determines the
effectiveness of antimicrobial
solutions against designated
prototype viruses. The
effective antimicrobial
concentration should be
determined using cell cultures
as the host system for specific
viruses. For special
applications of virucides, such
as inactivation of viruses in
contaminated liquid wastes,
and as a first stage in
determining virucidal potential
26
Manufacturer
REFERE
NCE
TITLE
PT

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
of liquid chemical germicides,
liquid hand soaps, OTC
topicals or other skin products.
TYPE OF
REFERENCE
SOURCE
ASTM
E105397(2002)

for Efficacy of Virucidal
Agents Intended for
Inanimate
Environmental
Surfaces
1-5
Manufacturer
ASTM
E1054-02
Standard Test
Methods for Evaluation
of Inactivators of
1-5
Laboratory test method.

Evaluates the virucidal efficacy
of liquid, aerosol, or trigger
spray antimicrobial solutions
on inanimate nonporous
environmental surfaces,
against designated prototype
viruses. The effective
antimicrobial concentration
should be determined utilizing
cell cultures as the host
system for specific viruses.
Efficacy is measured by a
percentage reduction in titer.
Effectiveness of procedures
and agents for inactivating
(neutralizing, quenching) the
microbiocidal properties of
antimicrobial agents and to
ensure that no components of
the neutralizing procedures
and agents, themselves, exert
an inhibitory effect on
microorganisms targeted for
recovery.
ASTM
E1115-02
Test Method for

Evaluation of Surgical
Hand Scrub
Formulations
for Efficacy of
Sanitizers
Recommended for
Inanimate Non-Food
Contact Surfaces
of a Evaluation of a
Preoperative,
Precatheterization, or
Preinjection Skin
Preparations
Test Method for
Evaluation of the
Effectiveness of Health
Care Personnel
Handwash
Formulations
1-5
Own searches
1-5
Own searches
1-5
Own searches
1-5
Own searches
1-5
Own searches
ASTM
E1153-03
ASTM
E117301e1
ASTM
E1174-06
ASTM
27
Own searches
REFERE
NCE
TITLE
E1327-07
for Evaluation of
Antimicrobial
Handwash
Formulations by
Utilizing Fingernail
Regions
for Neutralization of
Virucidal Agents in
Virucidal Efficacy
Evaluations
for Evaluation of First
Aid Antiseptic Drug
Products
for Determination of
Effectiveness of
Sterilization Processes
for Reusable Medical
Devices
to Determine Efficacy
of Disinfection
Processes for
Reusable Medical
Devices (Simulated
Use Test)
for Determining the
Virus-Eliminating
Effectiveness of Liquid
Hygienic Handwash
and Handrub Agents
Using the Fingerpads
of Adult Volunteers
for Evaluation of
Antimicrobial
Formulations by the
Agar Patch Technique
ASTM
E1482-04
ASTM
E1589-05
ASTM
E176695(2002)
ASTM
E183796(2002)
ASTM
E1838-02
ASTM
E1882-05
PT

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
1-5
Own searches
1-5
Own searches
1-5
Own searches
1-5
Own searches
1-5
Own searches
1-5
This test method determines

the antibacterial activity and
persistence of test
formulations, as measured by
the inhibition of a test
organism on an agar surface
exposed to test sites on
human skin treated with the
formulations. This procedure
can be used to evaluate
formulations containing
ingredients intended to inhibit
growth of bacteria on intact
skin and measures the
difference, post-productexposure, between numbers of
28
Own searches
REFERE
NCE
TITLE
PT
ASTM
E1883-02

for Assessment of an
Antibacterial
Handwash Product by
Multiple Basin Wash
Technique
for Evaluation of
Handwashing
Formulations for VirusEliminating Activity
Using the Entire Hand
Standard Quantitative
Carrier Test Method to
Evaluate the
Bactericidal,
Fungicidal,
Mycobactericidal, and
Sporicidal Potencies of
Liquid Chemical
Microbicides
1-5
Own searches
1-5
Own searches
ASTM
E2011-99
ASTM
E2111-05
1-5

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
bacterial colonies on active
test formulation plates and
numbers on control plates,
expressed as percent
inhibition.
This procedure may also be
used to test for persistence of
activity, as a function of time
elapsed between application of
active test formulation and
application of active test
plates. Because no procedure
for neutralization of the
antimicrobial action of active
ingredients can be included in
the test, the agar patch
method is limited to the extent
that results expressed as
percent inhibition do not
differentiate between
bacteriostatic and bacteriocidal
effects and, hence, must not
be portrayed as reductions.
This test can be performed

with or without a soil load to
determine the effect of such
loading on microbicide
performance. The soil load
developed for this test is a
mixture of three types of
proteins (high molecular
weight proteins, low molecular
weight peptides, and mucous
material) to represent the body
secretions, excretions, or other
extraneous substances that
chemical microbicides may
encounter under field
condititions.
This test method is designed
29
TYPE OF
REFERENCE
SOURCE
Own searches
REFERE
NCE
TITLE
PT
ASTM
E2197-02
Disk Carrier Test
Method for
Determining the
Bactericidal, Virucidal,
Fungicidal,
Mycobactericidal and
Sporicidal Activities of
Liquid Chemical
Germicides
1-5

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
for use in product development
and for the generation of
product potency data. This test
method is fully quantitative and
it also avoids any loss of viable
organisms through wash off. It
permits the loading of each
carrier with a known volume of
the test organism. The
incorporation of controls can
also determine the initial load
of colony forming units (CFU)
of organisms on the test
carriers and any loss in CFU
after the mandatory drying of
the inoculum. This test method
is designed to have survivors
and also to be used with a
performance standard. The
surviving microorganisms on
each test carrier are compared
to the mean of no less than
three control carriers to
determine if the performance
standard has been met. To
allow proper statistical
evaluation of results, the size
of the test inoculum should be
sufficiently large to take into
account both the performance
standard and the experimental
variation in the results.
The method is designed to
evaluate the ability of liquid
chemical germicides to
inactivate vegetative bacteria,
viruses, fungi, mycobacteria
and bacterial spores in the
presence of a soil load on disk
carriers that represent
environmental surfaces and
medical devices. It is also
designed to have survivors
that can be compared to mean
of no less than three control
carriers to determine if the
performance standard has
been met. For proper statistical
evaluation of the results, the
size of the test inoculum
should be sufficiently large to
take into account both the
30
TYPE OF
REFERENCE
SOURCE
Own searches
REFERE
NCE
TITLE
PT
ASTM
E2274-03

for Evaluation of
Laundry Sanitizers and
Disinfectants
for Determining the
Bacteria-Eliminating
Effectiveness of
Hygienic Handwash
and Handrub Agents
of Adult Subjects
Effectiveness of
Cleaning Processes
Instruments Using a
Microbiologic Method
(Simulated Use Test)
1-5
Own searches
1-5
Own searches
1-5
Own searches
ASTM
E2315-03
Standard Guide for

Assessment of
Antimicrobial Activity
Using a Time-Kill
Procedure
1-5
ASTM
E2361-04
Standard Guide for

Testing Leave-On
Products Using In-Situ
Methods
1-5
ASTM
E227603e1
ASTM
E2314-03

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
performance standard and the
experimental variation in the
results. The test protocol does
not include any wiping or
rubbing action. It is, therefore,
not designed for testing
germicide-soaked wipes.
This guide covers examples of

a basic method to measure the
changes of a population of
aerobic microorganisms within
a specified sampling time
when tested against
antimicrobial test materials in
vitro. Several options for
organism selection and
growth, inoculum preparation,
sampling times and
temperatures are provided.
Antimicrobial activity of specific
materials, as measured by this
technique, may vary
significantly on variables
selected.
This guide covers test
methods and sampling
procedure options for leave-on
products (such as alcohol
hand rubs and lotions
containing antimicrobial
ingredients) for consumer and
31
TYPE OF
REFERENCE
SOURCE
Own searches
Own searches
REFERE
NCE
TITLE
PT
ASTM
E2362-04

Evaluation of Presaturated or
Impregnated
Towelettes for Hard
Surface Disinfection
1-5
Own searches
ASTM
E2406-04

for Evaluation of
Disinfectants for Use
in High Efficiency
Washing Operations
1-5
Own searches
ASTM
E640-06

for Preservatives in
Water-Containing
Cosmetics
1-5
ASTM
WK12880
Standard Guide for

Evaluation of Clean
Room Disinfectants
Standard Guide for
Selecting Test
Methods to Determine
the Efficacy of
1-5
Own searches
1-5
Own searches
ASTM
WK4751

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
hospital personnel. These
products are distinguished
from conventional washing and
scrubbing preparations in that
they do not rely on the rinsing,
physical removal, and
antimicrobial action in
determining their
effectiveness. Although
agitation and friction may
serve to release organisms
from the skin and folds and
crevices, organisms are then
killed in situ and are not rinsed
from the skin surface before
sampling. Appropriate test
methods for the hands have
been published, while other
sampling methods will be
needed for testing body areas
other than the hands.
This test method should be

used to determine if a
preservative or preservative
system has application for the
preservation of water-miscible
cosmetic products.
It sets minimal requirements
for preservative performance
in model formulations.
32
TYPE OF
REFERENCE
SOURCE
Own searches
REFERE
NCE
ASTM
WK9062
ASTM
WK9378
Bechert
et al.,
Nature
Medicine
6, 10531056
(2000)
TITLE
and Other Chemicals

for Sanitization of
Produce
Guideline for
Evaluation of Residual
Effectiveness of
Antibacterial Personal
Cleansing Products
for Determining the
Fungus-Eliminating
Effectiveness of
Hygienic Handwash
and Handrub Agents
of Adults
A new method for
screening anti-infective
biomaterials
PT

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
1-5
Own searches
1-5
Own searches
1-5
The test is based on the

release of vital daughter cells
from the sample surface into
the surrounding. The
proliferation activity of these
daughter cells, which are
responsible for infection
development, can be
monitored in a time course.
Antimicrobial activity is
monitored by the time needed
to reach a defined optical
density, which is dependent on
the number of released
daughter cells. All
measurements are performed
in comparison to an untreated
control (without antimicrobial
additive). The difference
between the reference (A) and
the test sample (B) to reach
the threshold OD gives the
degree of antimicrobial activity.
If all bacteria on the surface of
the material are prevented
from multiplying, no daughter
cells are produced and the test
object is considered
bactericidal. Materials can also
be antimicrobial, which means
that not all cells on the test
surface are prevented from
growing. Some cells are able
to divide and release daughter
cells into the surroundings,
which are then optically
33
Test institute
REFERE
NCE
TITLE
PT

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
registered in a so-called
growth curve. If surviving
daughter cells are grown under
controlled conditions over 48 h
(observation time), a higher
turbidity and therefore a bigger
signal is generated. At the
same time only vital and
proliferative cells are
accounted for.
TYPE OF
REFERENCE
SOURCE
In particular antimicrobial
samples will release daughter
cells into the surrounding.
Hence, microbial growth is first
observed noticeably later. This
right-shift towards longer times
is indicative for the
antimicrobial efficacy of the
tested samples. The so-called
onset OD serves as a
quantifiable parameter and is
equivalent to the required
number of hours required for
the surviving daughter cells to
grow to a predefined optical
density (OD = 0.2).
A material is defined to be
antimicrobial only if the
formation of at least 99.9%
(which is equivalent to a net
onset-OD of 6 hours and is
similar to a killing rate of 3
log10 steps) of the daughter
cells during the challenge time
is prevented in comparison
with the blank sample.
BS
6734:200
4
CEN
158835:2005
General Purpose
Disinfection Test
1-5
Washer-disinfectors Part 5: Test soils and

methods for
demonstrating
cleaning efficacy
1-5
Marketed worldwide by Ciba

Specialty Chemicals under the
tradename NumetrikaTM.
Mycobacterium fortuitum
Also ISO method. Acceptance

criteria are included, based on
visual inspection and/or a
microbiological end-point as
stated for each method. Where
chemical detection of residual
soiling is required/sought,
34
Manufacturer
Own searches
REFERE
NCE
TITLE
PT
EN
1040:200
5
Chemical disinfectants
and antiseptics Quantitative
suspension test for the
evaluation of basic
bactericidal activity of
chemical disinfectants
and antiseptics - Test
method and
requirements (phase
1)
1-5
EN
12353:20
06
EN
1275:200
5
and antiseptics Preservation of test
organisms used for the
determination of
bactericidal,
mycobactericidal,
sporicidal and
fungicidal activity
evaluation of basic
fungicidal or basic
yeasticidal activity of
1-5

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
methods can be
complemented by the specific
determination of a residual
component of the applied test
soil.
Basic bacterial activity against
of a test material against
Staphylococcus aureus (ATCC
6538) and Pseudomonas
aeruginosa (ATCC 15442). A
test suspension of bacteria is
added to a prepared sample of
the product under test. The
mixture is maintained at 20 oC.
At a specified contact time
chosen from one of the
following: 1 , 5, 15 , 30 , 45 or
60 minutes, an aliquot is taken.
The bactericidal action of this
aliquot is immediately
neutralised or suppressed by a
validated method. The method
of choice is dilutionneutralisation. If a suitable
neutraliser cannot be found,
membrane filtration is used.
The number of surviving
bacteria in each sample is
determined and the reduction
in viable counts calculated.A
criterion for activity by this test
method is that the test material
should demonstrate at least a
5-log reduction in viable counts
of the test organisms in 60
minutes.
General guidance
TYPE OF
REFERENCE
SOURCE
Industry
organizaton
manufacturer
Communication
with UK (HSE)
TM II05 (Fr)
TNsG PE
(updated)
Industry
organizaton
TM II05 (Fr)
TNsG PE
1-5
Basic fungicidal activity against

Candida albicans (ATCC
10231) and Aspergillus niger
(ATCC 16404). A test
suspension of yeast cells or
mould spores is added to a
prepared sample of the
35
Industry
organizaton
Manufacturer
REFERE
NCE
TITLE
PT
method and
requirements (phase
1)
EN
1276:199
7
evaluation of
and antiseptics used in
food, industrial,
domestic, and
institutional areas Test method and
requirements (phase
2, step 1)
1-5

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
product under test. The
mixture is maintained at 20 oC.
At a specified contact time
following 5, 15, 30 or 60
minutes, an aliquot is taken;
the fungicide action in this
portion is immediately
neutralised or suppressed by a
validated method. The method
of choice is dilutionneutralisation. If a suitable
neutraliser cannot be found,
membrane filtration is used.
The number of surviving yeast
cells or mould spores in each
sample is determined and the
reduction in viable counts
calculated.
The criterion for activity by this
test is that the test material
minutes.
Dilution/Neutralisation .
Efficacy determined by a
reduction in CFU.
TYPE OF
REFERENCE
SOURCE
Communication
with UK (HSE)
TM II05 (Fr)
TNsG PE
Industry
organizaton
Manufacturer
TM II05 (Fr)
TNsG PE
EN
12791:20
05
EN
and antiseptics Surgical hand
disinfection - Test
method and
requirement (phase
2/step 2)
1-5
Testing mainly bactericidal

activity; in vivo test; phase 2
step 2
Industry
organizaton/
Manufacturer
TM II05 (Fr)
TNsG PE
Industry
1-5
36
REFERE
NCE
TITLE
13610:20
02
- Quantitative
evaluation of virucidal
activity against
bacteriophages of
used in food and
industrial areas - Test
method and
requirements (phase
2, step 1)
evaluation of fungicidal
activity of chemical
disinfectants for
instruments used in
the medical area - Test
method and
requirements (phase
2, step 1)
and antiseptics Quantitative nonporous surface test for
the evaluation of
bactericidal and/or
fungicidal activity of
used in food,
industrial, domestic
and institutional areas
- Test method and
requirements without
mechanical action
(phase 2/step2)
EN
13624:20
03
EN
13697:20
01
PT

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
organizaton
TM II05 (Fr)
TNsG PE
1-5
Industry
organizaton/ma
nufacturer
TM II05 (Fr)
TNsG PE
1-5
Dilution/Neutralisation Method.
Efficacy measured by a
reduction in CFU.
Industry
organizaton
Manufacturers
TM II05 (Fr)
TNsG PE
EN
13704:20
02
- Quantitative
evaluation of sporicidal
disinfectants used in
food, industrial,
domestic and
1-5
Industry
organizaton
Manufacturer
TM II05 (Fr)
TNsG PE
37
REFERE
NCE
EN
13727:20
03
TITLE
requirements (phase
2, step 1)
evaluation of
for instruments used in
method and
requirements (phase
2, step 1)
PT

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
1-5
Efficacy determined by the

reduction in CFU counts
Industry
organizaton
Manufacturer
TM II05 (Fr)
TNsG PE
EN
14204:20
04
EN
14347:20
05
evaluation of
mycobactericidal
disinfectants and
antiseptics used in the
veterinary area - Test
method and
requirements (phase
2, step 1)
and antiseptics - Basic
sporicidal activity Test method and
requirements (phase
1)
1-5
Effiacy measured by reduction
in CFU.
Industry
organisation
Manufacturer
TM II05 (Fr)
TNsG PE
1-5
Basic sporicidal activity against

dormant spores of Bacillus
subtilis (ATCC 6633) and
Bacillus cereus (ATCC 12826).
A prepared sample of the
product under test is added to
a test suspension of bacterial
spores. The mixture is
maintained at 20 oC or any
other temperature to be
defined. At a specified contact
time chosen from one of the
following: 30, 60 and 120
minutes, an aliquot portion is
taken and the sporicidal as
well as sporistatic action in this
portion is neutralised. The
method of choice is dilutionneutralisation. The number of
surviving bacterial spores is
determined in parallel and the
38
Industry
organizaton
Manufactuer
Communication
with UK (HSE)
TM II05
TNsG PE
REFERE
NCE
TITLE
PT
EN
14348:20
05
evaluation of
mycobactericidal
disinfectants in the
medical area including
instrument
disinfectants - Test
methods and
requirements (phase
2, step 1)
and antiseptics Quantitative surface
test for the evaluation
of bactericidal activity
of chemical
disinfectants and
antiseptics used in
veterinary area on
non-porous surfaces
without mechanical
action - Test method
and requirements
(phase 2, step 2)
1-5
and antiseptics Virucidal quantitative
suspension test for
human medicine - Test
method and
requirements (phase
2, step 1)
1-5
EN
14349:20
07
EN
14476:20
05+A1:20
06

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
reduction in viable counts
calculated. The effectiveness
of neutralisation is controlled in
the test.
The criterion for activity by this
test is that the test material
minutes.Medical area
Veterinary area, Food,
industrial, domestic and
institutional hygiene
TYPE OF
REFERENCE
SOURCE
Industry
organizaton
Manufacturer
1-5
Non-porous surface test,

bactericidal, for disinfectants
used in the veterinary
applications
By one manufacturer, the test
material is deemed to have
passed the test and be
efficacious if it demonstrates a
log 4 or more reduction in
viable counts under the
conditions defined in the test.
Reduction in viable microbial
counts compared with water
controls.
Industry
organizaton
Manufacturer
TM II05 (Fr)
TNsG PE
Industry
organizaton
Manufacturer
TM II05 (Fr)
39
REFERE
NCE
TITLE
PT
EN
14561:20
06
and antiseptics Quantitative carrier
of bactericidal activity
method and
requirements (phase
2, step 2)
of fungicidal or
yeasticidal activity for
instruments used in
method and
requirements (phase
2, step 2)
evaluation of virucidal
disinfectants and
method and
requirements (phase
2, step 1)
Ophtalmic optics contact lense care
products antimicrobial
preservative efficacy
testing and guidance
on determining discard
rate
and antiseptics Application of
European Standards
for chemical
disinfectants and
antiseptics
1-5
EN
14562:20
06
EN
14675:20
06
EN
14730:
2000
prEN
14885:20
06

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
Industry
organizaton
Manufacturer
TM II05 (Fr)
1-5
Industry
organizaton
Manufacturer
TM II05 (Fr)
1-5
Industry
organizaton
Manufacturer
TM II05 (Fr)
1-5
Also ISO method.
1-5
Own searches
Industry
organizaton
Manufacturer
TM II05 (Fr)
EN
1499:199
and antiseptics -
1-5
Applied in medical area
40
Industry
organizaton
REFERE
NCE
TITLE
Hygienic handwash Test method and

requirements (phase
2/step 2)
PT

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
Manufacturer
TM II05 (Fr)
TNsG PE
EN
1500:199
7
and antiseptics Hygienic handrub Test method and
requirements (phase
2/step 2)
1-5
Industry
organizaton
Manufacturer
TM II05 (Fr)
TNsG PE
EN
1650:199
7
EN 1656
: 2000
disinfectants and
antiseptics used in
food, industrial,
domestic, and
requirements (phase
2, step 1)
evaluation of
veterinary field - Test
method and
requirements (phase
2/step 1)
1-5
Industry
organizaton
Manufacturer
TM II05 (Fr)
TNsG PE
1-5
reduction in CFU.
Manufacturers
Industry
organisation
TM II05 (Fr)
TNsG PE
EN
1657:200
and antiseptics -
1-5
By one manufacturer, the

product was to be deemed to
41
Manufacturers
REFERE
NCE
TITLE
5/AC:200
7
Quantitative
or yeasticidal activity
of chemical
disinfectants and
method and
requirements (phase
2, step 1)
MAFF
(1969)
NF T72230
August
1988
NF T72281
Septemb
er 1986
Not
available
Disinfectants for use

specifically against: a)
anthrax, brucellosis,
contagious bovine
pleuro-pneumonia and
glanders; b) For use
against tuberculosis; c)
For use against footand-mouth disease; d)
For use against fowl
pest (Newcastle
disease fowl plague).
Water-miscible,
neutralizable
antiseptics and
disinfectants used in
liquid form.
Determination of
sporicidal activity.
Dilution-neutralization
method.
Methods of airborne
disinfection of
surfaces. Determiation
of bactericidal,
fongicidal and
Assessment of the
biocide efficacy using
three algal species. Inhouse method
PT

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
have passed the test if it
demonstrated a 10E5
logarithmic reduction in
viability.
By one manufacturer a
reduction in viable microbial
counts is compared with water
controls.
The test material is deemed to
have passed the test and be
efficacious if it demonstrates a
log 4 or more reduction in
viable counts under the
conditions defined in the test
TYPE OF
REFERENCE
SOURCE
Industry
organizaton
TM II05 (Fr)
TNsG PE
1-5
TNsG PE
1-5
Manufacturer
1-5
TM II05 (Fr)
1-5
Various concentrations of
product was mixed with algal
suspensions (Chlorella
vulgaris
Navicula pelliculosa
Anabaena flos-aquae) in glass
conical flasks All flasks were
incubated and shaken in an
orbital shaker. Samples were
taken at 96h and the
42
Manufacturer
REFERE
NCE
TITLE
PT
Not
available
Disinfectant for
swimming pools for
control of bacteria,
fungi and algae.
Determination of
Minimum Inhibitory
Concentration Against
Bacteria, Fungi, Algae
and Cyanobacteria (in
house method)
1-5
Not
available
Disinfectant treatment
of waste water. A
laboratory study
involving disinfection
of untreated river
water, as a surrogate
for a full field trial. (inhouse methods)
of waste water. Full
scale field trial
1-5
of waste water.
Inactivation of enteric
micro-organisms in
tertiary treated
municipal waste
waters (in-house
methods)
1-5
Not
available
Not
available
1-5

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
chlorophyll a concentration
determined. Efficacy was
measured by determining the
EC50 value obtained for algae.
Using a growth inhibition test
method for recording MIC
values to test the efficacy of
the product at various diluted
concentrations. MIC value was
determined using microdilution
technique in microtiter plates
Tests were carried out in
quadruplicate. The ppm level
of the product in the last
microtiter well demonstrating
no growth was determined as
the MIC for the product against
that microorganism.
Several concentrations of the
product were added to river
water and allowed to react for
30 mins. Total counts and
coliforms were determined.
Efficacy was determined
based on reduction in CFU
counts.
A product was used to treat
waste water at 3
concentrations under real field
conditions involving a waste
water treatment plant; variable
flow and bacterial load, and
operating temperatures.
Efficacy was measured based
on a log removal of bacteria
after 15 and 30 mins.
Experiments were conducted
to investigate in pilot scale the
disinfection efficiency against
enteric micro-organisms in
tertiary treatment waste waters
and the effect of dose and
contact time on disinfection
efficiency. After incubation
bacterial colonies were
counted and microbial
numbers calculated as cfu/
100 ml.
Efficacy was measured by
percentage reductions of
microorganisms after 8 and 18
minutes contact time.
43
TYPE OF
REFERENCE
SOURCE
Manufacturer
Manufacturer
Manufacturer
Manufacturer
REFERE
NCE
TITLE
PT
Not
available
of waste water. Pilot
studies to assess the
disinfection
performance of on a
physicochemical
wastewater effluent.
1-5
Not
available
Swimming pool
disinfectant:
Residential spa field
test
1-5

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
A purpose built disinfection,
continuous flow, Pilot Plant
was specially constructed and
installed at a functioning
wastewater treatment plant.
Prior to installation the
wastewater was treated only
by chemically assisted
sedimentation, making
disinfection particularly difficult.
As part of this pilot study,
modifications to the Plant and
process were explored and
adopted on the basis of
experience and experimental
data using standard
methodologies. Disinfection
efficiency under a number of
conditions and wastewater
loads were explored. Method
for recording faecal coliforms:
American Public Health
association; American Water
Works Association and Water
Environment federation (1998)
Standard Methods for the
Examination of Water and
Wastewater 20th Ed.;
Washington DC, USA; Method
9222D. Method for recording
Enterococcus, coliphage
Clostridium
according to laboratory
methods. Efficacy was
determined by measured Log
reductions (or removal) in
counts. Colonies were counted
before and after disinfection.
A residential spa was used to
test the efficacy of the product
in typical field conditions. The
maintenance pattern used was
considered to be typical of the
average spa owner, i.e.
conforming to minimum and
not optimum use pattern and
maintenance procedures.
Bacteriological analysis was
conducted on 150 samples
were taken over the 90 day
test period. Efficacy was
determined by a reduction in
44
TYPE OF
REFERENCE
SOURCE
Manufacturer
Manufacturer
REFERE
NCE
TITLE
PT
OECD
(ENV/JM/
BCID(200
7)2)
Proposed
development of an
OECD Guidance
Document for
establishing the
efficacy of biocides
used in swimming
pools and spas
Biocidal Efficacy of a
Flocculating
Emergency Water
Purification Tablet.
1-5
evaluation of
bactericidal activity
against Legionella
pneumophila of
for aqueous systems Test method and
requirements (phase
2, step 1)
of mycobactericidal or
tuberculocidal activity
of chemical
disinfectants used for
instruments in the
medical area - Test
method and
requirements (phase
2, step 2)
for Quantification of
Pseudomonas
1-5
Own searches
1-5
Manufacturer
Powers
et al.
Applied
and
Environm
ental
Microbiol
ogy, 60:
2316
2323
(1994)
prEN
13623
prEN
14563
ASTM
E2562-07
1-5

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
cfu/100ml.
TYPE OF
REFERENCE
SOURCE
Own searches
Biocidal emergency water

purification tablets were tested
for bactericidal, virucidal and
cysticidal efficacy in water at
temperatures ranging from 5 to
25C to validate and verify the
effectiveness of the tablets for
the destruction and removal of
microorganisms. Counts of
surviving organisms were
compared with the initial
counts to calculate the logreduction. Replicate results
were averaged
Manufacturer
TM II05 (Fr)
1-5 +
12
This test method specifies the

operational parameters
required to grow a repeatable
45
Own searches
REFERE
NCE
TITLE
PT
aeruginosa Biofilm
Grown with High
Shear and Continuous
Flow using CDC
Biofilm Reactor
ASTM
D2574-06
ASTM
D478301e1

for Resistance of
Emulsion Paints in the
Container to Attack by
Microorganisms
Standard Test
Methods for
Resistance of
Adhesive Preparations
in Container to Attack
by Bacteria, Yeast,
and Fungi

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
Pseudomonas aeruginosa
biofilm under high shear (1).
The resulting biofilm is
representative of generalized
situations where biofilm exists
under high shear rather than
representative of one particular
environment. The biofilm
generated in the CDC biofilm
reactor is also suitable for
efficacy testing. 3 This test
method describes how to
sample and analyze biofilm for
viable cells. Biofilm population
density is recorded as log
colony forming units per
surface area. After the 48 h
growth phase is complete, the
user may add the treatment in
situ or harvest the coupons
and treat them individually.
This test method uses the
Centers for Disease Control
and Prevention (CDC) biofilm
reactor. The CDC biofilm
reactor is a continuously
stirred flow reactor with high
wall shear. Although it was
originally designed to model a
potable water system for the
evaluation of Legionella
pneumophila, the reactor is
versatile and may also be used
for growing and/or
characterizing biofilm of
varying species.
TYPE OF
REFERENCE
SOURCE
Own searches
Determination of the
resistance of liquid adhesive
preparations to microbial
attack in the container by
challenging adhesive
specimens with cultures of
bacteria, yeast, or fungi, and
checking for their ability to
return to sterility.
These test methods return
qualitative results
46
TNsG on Prod
Eval
REFERE
NCE
TITLE
PT
ASTM
E1259-05

Evaluation of
Antimicrobials in Liquid
Fuels Boiling Below
390C
ASTM
WK5097
Guideline For
Evaluating The
Potential For Decline
In Planktonic
Microorganism
Susceptability To
Antimicrobial
Compounds:
Healthcare
Applications
Not
available
Other in can
preservatives. Inhouse method based
on Antimicrobial
Preservative
Effectiveness
Challenge Test and
DAB 10 guidelines
Not
available
Preservative for
detergents. In-house
method based on
Antimicrobial

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
The procedure should be used
to evaluate the relative efficacy
of microbicides in liquid fuels
boiling below 390C. The
effect of environmental
conditions, such as a variety of
fuel additives, metal surfaces,
and climatology, are variables
that can be included in specific
tests using this protocol.
This method uses an agardilution procedure to determine
the Minimum Inhibitory
Concentration (MIC) and
subsequently to screen for the
development of decreased
susceptibility for test
formulations versus bacteria or
yeast species. The inocula
used on the second, third,
fourth, and fifth test cycles will
be prepared from the microbial
growth present at the previous
days end point;i.e., that agardilution plate containing the
highest concentration of the
test formulation (lowest
dilution) that allows microbial
growth.
TYPE OF
REFERENCE
SOURCE
The method was conducted by

inoculating mixed bacteria and
mixed fungi into a interior paint
formulation containing various
concentrations of the active
substance which was repeated
on a weekly basis. After 0, 7
14, 21 and 28 days samples of
the formulations were placed
in a preservative neutralizer
and then plated. After plate
incubation the number of
surviving organisms was
compared to the control. The
criteria for measuring good
preservation efficacy was a
microbial count of <10 cfu/ml
at day 7, 14 , 21 and 28 days
inoculating mixed bacteria and
mixed fungi into a washing up
liquid formulation containing
Manufacturer
47
Own searches
Own searches
Manufacturer
REFERE
NCE
TITLE
PT
Preservative
Effectiveness
Challenge Test and
DAB 10 guidelines
SABS
1102
(1987)
SM020
(in
house)
Bacterial efficacy of
biocides used in waterbased emulsion paints
S&M Fako test
SM021
(in
house)
S&M KOKO test

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
various concentrations of the
active substance which was
repeated on a weekly basis.
After 0, 7 14, 21 and 28 days
samples of the formulations
were placed in a preservative
neutralizer and then plated.
After plate incubation the
number of surviving organisms
was compared to the control .
The criteria for measuring
good preservation efficacy was
a microbial count of <10 cfu/ml
at day 7, 14 , 21 and 28 days.
Efficacy test for in can
preservatives in paints
(emulsion) against bacteria.
Preserving effect of chemical
preservatives in water
containing coatings, adhesives
and other water containing
technical emulsions or
dispersions (in can).
Test batch: unpreserved
samples with different
concentrations of test
preservatives, inoculated and
streaked on agar plates.
Test germ: G+, G-, Moulds,
Yeasts
"Well preserved" if after 6w no
microbial growth can be
observed.
Corresponds to 2 years of
microbial stability.
4 evaluation levels
Preservatives in cosmetic
formulations, e g creams,
shampoos etc (in can)
Yeasts
"Well preserved" if after 6w no
microbial growth can be
observed.
Corresponds to 30 months of
4 evaluation levels
48
TYPE OF
REFERENCE
SOURCE
TNsG on Prod
Eval
Manufacturer
Manufacturer
REFERE
NCE
TITLE
PT
SM026
(in
house)
S&M Betoko test:

preserving effect of
chemical preservatives
in concrete admixtures
SM029
(in
house)
S&M FeuTuKo test
SM036
(in
house)
S&M WiWako test
SM037
(in
house)
S&M TaBaKo test
SM044
(in
house)
in household
formulations

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
preservatives in concrete
admixtures (in can
preservation).
Yeasts
Free of growth during 4 weeks:
"Well preserved"
(4 evaluation levels)
Corresponds to 1 year of
microbiological stability
Preservatives in wet tissues in
their original packaging.
Inoculation of whole packaging
and after 3w of incubation,
pieces of tissue is placed on
agar plates.
Yeasts
3d bacteria, 7d moulds. 4
evaluation levels.
Preservation of fountain
solutions for offset printing.
Yeasts
12 cycles
4 evaluation levels.
Preservation of paint baths.
Yeasts
12 cycles. 4 evaluation levels.
preservatives in household
formulations, e g softener,
washing up liquids, all purpose
cleaner (in can preservation).
49
TYPE OF
REFERENCE
SOURCE
Manufacturer
Manufacturer
Manufacturer
Manufacturer
Manufacturer
REFERE
NCE
TITLE
PT
NF X41520
March
1968
Protection. Testing
method for resistance
of paints to
microorganisms and
their protective power.
Standard practice for
determining resistance
of synthetic polymeric
materials to fungi
6+
7
ASTM
G2196(2002)

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
Yeasts
Free of growth during 6 weeks:
"Well preserved". (4 evaluation
levels). Corresponds to 30
months of microbiological
stability
TYPE OF
REFERENCE
SOURCE
TNsG on Prod
Eval
TMII05
This practice covers

determination of the effect of
fungi on the properties of
synthetic polymeric materials
in the form of molded and
fabricated articles, tubes, rods,
sheets, and film materials.
Conditions favorable for attack,
namely, a temperature of 2 to
38C (35 to 100F) and a
relative humidity of 60 to 100
% need to be established.
Expected effects include
surface attack, discoloration,
loss of transmission (optical),
and removal of susceptible
plasticizers, modifiers, and
lubricants, resulting in
increased modulus (stiffness),
changes in weight,
dimensions, and other physical
properties, and deterioration of
electrical properties such as
insulation resistance, dielectric
constant, power factor, and
dielectric strength.
Since attack by organisms
involves a large element of
chance due to local
accelerations and inhibitions,
the order of reproducibility may
be rather low. To ensure that
estimates of behavior are not
too optimistic, the greatest
observed degree of
50
Own searches
REFERE
NCE
TITLE
PT
ASTM
D327300(2005)

for Resistance to
Growth of Mold on the
Surface of Interior
Coatings in an
Environmental
Chamber

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
deterioration should be
reported.
Conditioning of the specimens,
such as exposure to leaching,
weathering, heat treatment,
etc., may have significant
effects on the resistance to
fungi. Determination of these
effects is not covered in this
practice.
This test method describes a
small environmental chamber
and the conditions of operation
to evaluate reproducibly in a 4week period the relative
resistance of paint films to
surface mold fungi, mildew
growth in a severe interior
environment. The apparatus is
designed so it can be easily
built or obtained by any
interested party and will
duplicate results obtained in a
large tropical chamber.
An accelerated test for
determining the resistance of
interior coatings to mold
growth; useful in estimating
the performance of coatings
designed for use in interior
environments that promote
mold growth and in evaluating
compounds that may inhibit
such growth and the aggregate
levels for their use.
Used to evaluate the
comparative resistance of
interior coating to accelerated
mildew growth. Performance at
a certain rating (in accordance
with Test Method D3274) does
not imply any specific period of
time for a fungal free coating.
However, a better rated
coating nearly always performs
better in actual end use. This
test method is intended for the
accelerated evaluation of an
interior coatings' resistance to
fungal defacement. Use of this
test method for evaluating
exterior coatings' performance
51
TYPE OF
REFERENCE
SOURCE
Own searches
REFERE
NCE
TITLE
PT
ASTM
D345686(2002)

Determining by
Exterior Exposure
Tests the Susceptibility
of Paint Films to
Microbiological Attack
ASTM
Standard Test

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
has not been validated, nor
have the limitations for such
use been determined. Any
accelerated weathering
(leaching, weathering machine
exposure, etc.,) should be
reported and should also bear
reference to the fact that it is
beyond the current scope of
this test method.
Temperature and humidity
must be effectively controlled
within the relatively narrow
limits specified in order for the
chamber to function
reproducibly during the short
test period. Severity and rate
of mold growth on a film is a
function of the moisture
content of both the film and the
substrate. A relative humidity
of 95 to 98 % at a temperature
of 32.5+ 1oC (90+ 2oF ) is
necessary for test panels to
develop rapidly and maintain
an adequate moisture level to
support mold growth.
This practice provides
guidelines for determining the
susceptibility of paint films to
microbiological attack on
exterior exposure. The degree
to which microbiological
discoloration occurs is the
primary concern. This practice
covers the preparation of
coatings for testing, their
application on substrates, and
the arrangement of the coated
panels on exterior test fences
to determine the degree of
microbiological attack that may
occur on the surface of the
coatings over a period of time.
This practice is intended to
provide guidelines for, and a
discussion of, the various
factors critical in selection of
exterior coatings resistant to
discoloration or disfigurement
by algae and fungi.
Tests the ability of adhesive
52
TYPE OF
REFERENCE
SOURCE
Own searches
Own searches
REFERE
NCE
TITLE
D4300-01
Methods for Ability of

Adhesive Films to
Support or Resist the
Growth of Fungi
PT
ASTM
D558997(2002)

for Determining the
Resistance of Paint
Films and Related
Coatings to Algal
Defacement
ASTM
D559000(2005)

for Determining the
Resistance of Paint
Films and Related
Coatings to Fungal
Defacement by
Accelerated FourWeek Agar Plate
Assay

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
films to inhibit or support the
growth of selected fungal
species growing on agar plates
by providing means of testing
the films on two agar
substrates, one which
promotes microbial growth,
and one which does not.
constituents.
These test methods are not
appropriate for all adhesives.
The activity of certain biocides
may not be demonstrated by
these test methods as a result
of irreversible reaction with
some of the medium. As an
example, quaternary
ammonium compounds are
inactivated by agar. A test
method is included for use with
low-viscosity adhesives along
with an alternative method for
use with mastic-type
adhesives.
This test method covers an
accelerated method for
determining the relative
resistance of a paint or coating
film to algal growth.
This test method should not be
used as a replacement for
exterior exposure since many
other factors, only a few of
which are listed will affect
those results.
resistance of two or more
paints or coating films to fungal
growth.
This test method should not be
used as a replacement for
exterior exposure (that is,
Practice D 3456) since many
other factors, only a few of
which are listed will affect
those results.
Comparative evaluation of
different coating formulations
for their relative performance
53
TYPE OF
REFERENCE
SOURCE
Own searches
Own searches
REFERE
NCE
TITLE
PT
ASTM
E142899(2004)

for Evaluating the
Performance of
Antimicrobials in or on
Polymeric Solids
Against Staining by
Streptoverticillium
reticulum (A Pink Stain
Organism)
ASTM
E2196-07

for Quantification of a
Pseudomonas
aeruginosa Biofilm
Grown with Shear and
Continuous Flow
Using a Rotating Disk
Reactor
ASTM
G2996(2002)

Determining Algal
Resistance of Plastic
Films

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
under a given set of
conditions. It does not imply
that a coating that resists
growth under these conditions
will necessarily resist growth in
the actual application.
Round-robin testing of this test
method versus exterior
exposure is planned.
This test method provides a
technique for evaluating
antimicrobials in or on
polymeric solids against
staining by Streptoverticillium
reticulum, and should assist in
the prediction of performance
of treated articles under actual
field conditions. Conditioning
of the specimens, such as
exposure to leaching,
weathering, and heat
treatment, may have
significant effects on
performance of antimicrobials
against staining. Determination
of these effects is not included
in this test method. This test
method is also not suitable for
evaluating dark-pigmented test
samples.
This test method is used for
growing a repeatable
biofilm in a continuously stirred
flow reactor. In addition, the
test method describes how to
sample and analyze biofilm for
viable cells. In this test
method, biofilm population
surface area.
susceptibility of plastic films to
the attachment and
proliferation of surface-growing
algae, produced in bodies of
water, such as swimming
pools, artificial ponds, and
irrigation ditches that are lined
with plastic films.
54
TYPE OF
REFERENCE
SOURCE
Own searches
Own searches
Own searches
REFERE
NCE
TITLE
PT
EN
15457:20
07
Paints and varnishes Laboratory method for

testing the efficacy of
film preservatives in a
coating against fungi
coating against algae
Standard
Recommended
Practice for
Conducting Exterior
Exposure Tests of
Paints on Wood
Manufacturer
Own searches
Wood preservatives Artificial weathering of

treated wood prior to
biological testing - UVradiation and waterspraying procedure
Wood preservatives Field test method for
determining the
relative protective
EN
15458:20
07
ASTM
D1006-93
CEN/TR
15046:20
05
CEN/TS
12037:20
03

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
Evaluating the degree and
permanency of protection
against surface growth of
algae afforded by various
additives incorporated in the
film.
Field test: aids in evaluating

the performance of house and
trim paints to new, previously
unpainted wood exposed to
the environment.
Exposures in several locations
with different climates which
represent a broad range of
anticipated service conditions
are recommended and several
years of repeat exposures are
needed to get an average
test result for a given location.
Solar radiation varies
considerably as function of
time of year, and can cause
large differences in the
apparent rate of degradation in
many polymers. Comparing
results for materials exposed
for short periods (less than
onee year) is not
recommended unless
materials are exposed at the
same time in the same
location.
Pretreatment procedure before
efficacy testing
TYPE OF
REFERENCE
SOURCE
Own searches
Own searches
TM II05 (Fr)
55
REFERE
NCE
CTBABIO-E
001
CTBABIO-E
002
CTBABIO-E
003
CTBABIO-E
004
CTBABIO-E
005
CTBABIO-E
006
CTBABIO-E
007
CTBABIO-E
008
CTBABIO-E
009
CTBABIO-E
010
EN
113:1996
/A1:2004
TITLE
effectiveness of a
wood preservative
exposed out of ground
contact - Horizontal
lap-joint method
Field ageing test on
treated soils
PT

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
TM II05 (Fr)

treated masonry
TM II05 (Fr)
Field test on bait

system for termite
control
Laboratory tests for
bait system for termite
control
bait system for termite
control
Action of U.V. light on
physico-chemical
barrier for termite
Action of alcalin
conditions on physicochemical barrier for
termite control
physico-chemical
barrier for termite
control
Forced test on termite
bait control
TM II05 (Fr)
TM II05 (Fr)
TM II05 (Fr)
TM II05(Fr)
TM II05 (Fr)
TM II05 (Fr)
TM II05 (Fr)
Choice test on termite

bait control
TM II05 (Fr)
Wood preservatives Test method for

determining the
protective
effectiveness against
wood destroying
basidiomycetes Determination of the
toxic values
Laboratory test. Efficacy

criteria based on weight loss of
untreated specimen.
TNsG on Prod
Eval
TM II05 (Fr)
NL guidance
UK guidelines
Test institute
Manufacturer
EN
117:2005
Wood preservatives Determination of toxic
TNsG on Prod
Eval
56
REFERE
NCE
TITLE
PT

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
values against
Reticulitermes species
(European termites)
(Laboratory method)
TYPE OF
REFERENCE
SOURCE
TM II05 (Fr+De)
NL guidance
UK guidelines
EN
118:2005
Wood preservatives Determination of

preventive action
against Reticulitermes
species (European
termites) (Laboratory
method)
TNsG on Prod
Eval
TM II05 (Fr+De)
NL guidance
UK guidelines
EN
12490:19
98
EN
1390:200
6
EN 152-1
(1988)
EN 1522:1988/A
C1:1989
Durability of wood and

wood-based products Preservative-treated
solid wood Determination of the
penetration and
retention of creosote in
treated wood
Wood preservatives Determination of the
eradicant action
against Hylotrupes
bajulus (Linnaeus)
larvae - Laboratory
method
TM II05 (Fr)
TNsG Prod Eval
Test methods for wood

preservatives Laboratory method for
determining the
preventive
effectiveness of a
preservative treatment
against blue stain in
service - Part 1:
Brushing procedure
determining the
protective
effectiveness of a
against blue stain in
service - Part 2:
Application by
TM II05 (Fr)
NL guidance
UK guidelines
TNsG on Prod
Eval
TM II05 (Fr)
NL guidance
UK guidelines
TNsG on Prod
Eval
TM II05 (Fr)
NL guidance
UK guidelines
57
REFERE
NCE
EN 201:1992
EN 202:1993
EN
252:1989
/AC1:198
9
TITLE
methods other than

brushing
protective
Lyctus Brunneus
(Stephens) - Part 1:
Application by surface
treatment (laboratory
method)
protective
Lyctus brunneus
(Stephens) - Part 2:
Application by
impregnation
(Laboratory method)
Field test method for
determining the
relative protective
effectiveness of a
wood preservative in
ground contact
PT

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
TNsG on Prod
Eval
TM II05 (Fr)
NL guidance
UK guidelines
TNsG on Prod
Eval
TM II05 (Fr)
NL guidance
UK guidelines
Field test, in ground contact
TNsG on Prod
Eval
Test institute
NL guidance
UK guidelines
TM II05 (Fr)
EN
275:1992

protective
marine borers
Marine field test, 5 year

minumum
TNsG on Prod
Eval
Test institute
TM II05 (Fr)
NL guidance
UK guidelines
EN
Wood preservatives -
TNsG on Prod
58
REFERE
NCE
TITLE
330:1993

determining the
relative protective
effectiveness of a
wood preservative for
use under a coating
and exposed out of
ground contact: L-joint
method
PT

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
Eval
Test institute
TM II05 (Fr)
NL guidance
UK guidelines
EN 3501:1994
EN 3502:1994
EN 3511:2007
EN 3512:2007
EN
370:1993

wood-based products Natural durability of
solid wood - Part 1:
Guide to the principles
of testing and
classification of the
natural durability of
wood
Guide to natural
durability and
treatability of selected
wood species of
importance in Europe
Classification of
preservative
penetration and
retention
Guidance on sampling
for the analysis of
preservative-treated
wood
eradicant efficacy in
preventing emergence
of Anobium punctatum
TM II05 (Fr)
TM II05 (Fr)
TM II05 (Fr)
TM II05 (Fr)
TNsG on Prod
Eval
TM II05 (Fr)
59
REFERE
NCE
TITLE
PT

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
(De Geer)
TYPE OF
REFERENCE
SOURCE
NL guidance
UK guidelines
EN
460:1994
EN 461:2005
EN 462:2006
EN
47:2005/
AC:2007

solid wood - Guide to
the durability
requirements for wood
to be used in hazard
classes
preventive action
against Hylotrupes
bajulus (Linnaeus) Part 1: Larvicidial
effect (Laboratory
method)
TM II05 (Fr)
TNsG on Prod
Eval

preventive action
against Hylotrupes
bajulus (Linnaeus) Part 2: Ovicidal effect
(laboratory method)
toxic values against
larvae of Hylotrupes
bajulus (Linnaeus) (Laboratory method)
TM II05 (Fr+De)
NL guidance
UK guidelines
TM II05 (De)
NL guidance
TNsG on Prod
Eval
TM II05 (Fr+De)
NL guidance
UK guidelines
EN
48:2005

eradicant action
against larvae of
Anobium punctatum
(De Geer) (laboratory
method)
TNsG on Prod
Eval
TM II05 (Fr+De)
NL guidance
UK guidelines
EN 491:2005

protective
Anobium punctatum
TNsG on Prod
Eval
TM II05 (Fr+De)
60
REFERE
NCE
EN 492:2005
EN
73:1988/
AC:1992
TITLE
(De Geer) by egglaying and larval

survival - Part 1:
treatment (Laboratory
method)
protective
Anobium punctatum
(De Geer) by egglaying and larval
survival - Part 2:
Application by
impregnation
(Laboratory method)
Wood preservatives Accelerated ageing of
biological testing Evaporative ageing
procedure
PT

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
NL guidance
UK guidelines
TNsG on Prod
Eval
TM II05 (Fr+De)
NL guidance
UK guidelines

efficacy testing
TNsG on Prod
Eval
TM II05 (Fr)
NL guidance
UK guidelines
EN
84:1997

biological testing Leaching procedure

efficacy testing
TNsG on Prod
Eval
TM II05 (Fr)
NL guidance
UK guidelines
EN/TR
14734:20
04
EN/TR
14839:20
04
EN/TS
12037:20

wood-based products Determination of
treatability of timber
species to be
impregnated with
wood preservatives Laboratory method
preventive efficacy
against wood
destroying
basidiomycetes fungi
Own searches
Own searches
Field test, out of ground

contact
61
Test institute
REFERE
NCE
TITLE
03
determining the
relative protective
effectiveness of a
wood preservative
contact - Horizontal
lap-joint method
preventive
sapstain fungi and
mould fungi on freshly
sawn timber - Field
test
Wood preservatives Method for natural
preconditioning out of
ground contact of
treated wood
specimens prior to
biological laboratory
test
wood-based products Wood-based panels Method of test for
determining the
resistance against
wood-destroying
basidiomycetes
soft rotting micro-fungi
and other soil
inhabiting microorganisms
EN/TS
15082:20
05
EN/TS
15397:20
06
ENV
12038:20
02
ENV
807:2001
ENV
839:2002
EPPO
PP
1/194(2)
(1995)
JIS K
PT

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
UK guidelines
Own searches
Own searches
TM II05 (Fr)
UK guidelines
Laboratory test
TM II05 (Fr)
Test institute
NL guidance
UK guidelines

protective
wood destroying
basidiomycetes Application by surface
treatment
Blue-Stain fungi of
Softwood
TM II05 (Fr)
Own searches
Test methods for
Own searches
NL guidance
UK guidelines
62
REFERE
NCE
TITLE
1571:200
4
determining the
effectiveness of wood
preservatives and their
performance
requirements
wood-based products Definition of use
classes - Part 4 :
national declaration on
the situation of
biological agents
wood based products Preservative treated
solid wood - Part 3 :
wood preservation
performance and
treatment certificate Adaptation to France
metropolitan territory
and DOM
Protection - Termites Buildings protection
against termite
infestation
Wood preservatives.
Technical grade borax.
Specifications and
tests.
Wood preservatives.
Boric acid.
Specifications and
tests.
Wood preservatives.
Technical grade
pentachlorophenol.
Specificaitons and
tests.
Wood preservatives.
Technical grade
sodium
pentachlorophenate.
Specifications and
tests.
Wood preservatives.
Technical grade
sodium pentaborate.
Specifications and
tests.
Products for protecting
wood surfaces -
NF B50100-4
October
2007
NF B50105-3
February
2008
NF FD
X40-501
Novembe
r 2005
NF T72050
Septemb
er 1986
NF T72052
Septemb
er 1986
NF T72054
Septemb
er 1995
NF T72061
Septemb
er 1995
NF T72065
Septemb
er 1986
NF T72083/A1
PT

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
TM II05 (Fr)
TM II05 (Fr)
TM II05 (Fr)
TM II05 (Fr)
TM II05 (Fr)
TM II05 (Fr)
TM II05 (Fr)
TM II05 (Fr)
TM II05
63
REFERE
NCE
TITLE
Novembe
r 1979
Method of testing
resistance to
microorganisms
Wood preservatives.
Lasures. Natural
weathering test.
NF T72086
Septemb
er 1991
NF X41521 July
1968
NF X41541
Septemb
er 1994
NF X41547
Decembe
r 1992
NF X41548
Decembe
r 1992
NF X41555
August
1982
NF X41580 part
1-10
May 2006
NF XP
X41-540
Novembe
r 1995
PT

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
TM II05 (Fr)
Protection of wood.
Testing methods for
the corrosive action of
wood protection
products on metals.
Wood preservatives.
protective
termites of
products designed for
walls, foundations and
masonry. Laboratory
method.
Wood preservatives.
Determination of
longicide efficacy of
temporary wood
protectives for green
sawn timber.
Laboratory method.
Wood preservatives.
Determination of
fongicide efficacy of
temporary wood
protectives for fresh
cut wood billets.
Laboratory method.
Wood preservatives.
{chaetomium}
{globosum} kunze.
Soft rotting agent.
Wood preservatives
Physicochemical
testing
TM II05 (Fr)
TNsG on Prod
Eval
Wood preservatives.
Termites.
Determination of antitermites action for
products used in liquid
phase for ground
TM II05
TNsG on Prod
Eval
TM II05
TM II05 (Fr)
TM II05 (Fr)
TM II05 (Fr)
TM II05 (Fr)
64
REFERE
NCE
NF XP
X41-540
Novembe
r 1995
NF XP
X41-542
Septemb
er 1995
NF XP
X41-549
Decembe
r 1999
NF XP
X41-549
Decembe
r 1999
NWPC
1.4.1.1./7
0
NWPC
Standard
1.4.1.2./7
0
NWPC
Standard
1.4.1.3./7
9
TITLE
method).
Wood preservatives.
Termites.
Determination of antitermites action for
products used in liquid
phase for ground
method).
Wood preservatives.
Anti-termite treatment
product for floors,
masonry work.
Accelerated ageing
test of treated
materials prior to
biological testing.
Percolation test.
Wood preservatives Evaluation of fongicide
efficacy of temporary
wood protectives for
green sawn timber Site method
NWPC Standard for
testing of wood
preservatives.
Mycological test.
"Jordburk" method - A
soil block test with
wood-rotting
Basidiomycetes.
NWPC Standard for
testing of wood
preservatives.
Mycological test.
"Mullde" method - A
soil block test in
unsterile soil.
Mycological testing of
anti-stain
preservatives for
freshly sawn timber.
The Miniboard
PT

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
TNsG on Prod
Eval
TM II05 (Fr)
TM II05 (Fr)
TNsG on Prod
Eval
Test institute
Test institute
Test institute
65
REFERE
NCE
NWPC
Standard
1.4.2.1./7
1
NWPC
Standard
1.4.2.2./7
3
prEN 807
rev
AATCC
100 1999
AATCC
147 1998
AATCC
30 - 1999
ASTM
D457601(2006)
TITLE
method. (Mykologisk
provning av
trskyddsmedel mot
blnad och mgel p
nysgat virke.
Minibrdmetoden.)
Standard for testing of
wood preservatives
Mycological test. Field
test - A field test with
stakes.
wood preservatives
Marine test - A test
against marine
woodboring organisms
in sea water.
and other soil
inhabiting microorganisms
Antibacterial Finishes
on Textile Materials:
Assessment of
Antibacterial Activity
Assessment of Textile
Materials: Parallel
Streak Method
Antifungal Activity,
Assessment on Textile
Materials:
Mildew and Rot
Resistance of Textile
Materials
for Mold Growth
Resistance of Wet
Blue
PT

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
Test institute
Test institute
Own searches
Applied to textiles
Manufacturer
Applied to textiles
Manufacturer
Applied to textiles
Manufacturer
Determination of mold growth

resistance of wet blue subject
to storage and shipping
requirements and intended for
use in leather manufacturing,
assisting the prediction of
storage time before molding
occurs. This test method may
not be suitable to evaluate
fungicides that are inactivated
by proteins, including
alkyldimethylbenzyl
ammonium chlorides.
To allow use of this test
method by any laboratory,
66
REFERE
NCE
ASTM
E2149-01
TITLE
Determining the
of Immobilized
Under Dynamic
Contact Conditions
PT

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
flexibility has been permitted in
times, temperature, and
humidity of incubation,
inoculum, hide sampling area,
and choice of control. These
may be adjusted to fit local
conditions but must be
standardized.
Conclusions about mold
growth resistance are drawn
from the results by comparing
the test with a simultaneously
run control of known
resistance. Success or failure
is determined by the amount of
mold growth relative to the
control.
The degree of correlation
between this test and
commercial quantities of wet
blue in storage or shipment
situations, or both, has not
been fully determined.
Evaluates the resistance of
non-leaching antimicrobial
treated specimens to the
growth of microbes under
dynamic contact conditions.
This test determines the
antimicrobial activity of treated
specimen by shaking samples
of surface bound materials in a
concentrated bacterial
suspension for a one hour
contact time or other contact
times as specified by the
investigator. The suspension is
serially diluted both before and
after contact and cultured. This
dynamic shake flask test was
developed for routine quality
control and screening tests in
order to overcome difficulties
(including ensuring contact of
inoculum to treated surface) in
using classical antimicrobial
test methods to evaluate
substrate-bound
antimicrobials. This test also
allows for the versatility of
testing contamination due to
67
TYPE OF
REFERENCE
SOURCE
Manufacturer,
applied to
textiles
REFERE
NCE
TITLE
PT

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
such things as hard water,
proteins, blood, serum, various
chemicals, and other
contaminates or
physical/chemical stresses or
manipulations of the
specimens of interest.
The number of viable
organisms in the suspension is
determined and the percent
reduction is calculated based
on initial counts or on retrievals
from appropriate untreated
controls. his method is
intended for those surfaces
having a percent reduction
activity of 50 % to 100 % for
the specified contact time.
Surface antimicrobial activity is
determined by comparing
results from the test sample to
simultaneously run controls.
The presence of a leaching
antimicrobial is both pre- and
post-determined by the
presence of a zone of
inhibition.
TYPE OF
REFERENCE
SOURCE
ASTM
E2180-07

for Determining the
Activity of Incorporated
Antimicrobial Agent(s)
In Polymeric or
Hydrophobic Materials
This test method is designed

to evaluate (quantitatively) the
antimicrobial effectiveness of
agents incorporated or bound
into or onto mainly flat (two
dimensional) hydrophobic or
polymeric surfaces. The
method focuses primarily on
assessing antibacterial activity;
however, other
microorganisms such as yeast
and fungal conidia may be
tested using this method. The
vehicle for the inoculum is an
agar slurry which reduces the
surface tension of the saline
inoculum carrier and allows
formation of a "pseudobiofilm," providing more even
contact of the inoculum with
the test surface. This test
method facilitates the testing of
hydrophobic surfaces by
utilizing cells held in an agar
Own searches
68
REFERE
NCE
TITLE
PT
ASTM
E2471-05

for Using Seeded-Agar
for the Screening
Assessment of
In Carpets

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
slurry matrix.
This method can confirm the
presence of antimicrobial
activity in plastics or
hydrophobic surfaces and
allows determination of
quantitative differences in
antimicrobial activity between
untreated plastics or polymers
and those with bound or
incorporated low water-soluble
antimicrobial agents.
Comparisons between the
numbers of survivors on
preservative-treated and
control hydrophobic surfaces
may also be made. This test
method, as written, is
inappropriate to determine
efficacy against biofilm cells,
which are different both
genetically and metabolically
than planktonic cells used in
this test. The procedure also
permits determination of
"shelf-life" or long term
durability of an antimicrobial
treatment which may be
achieved through testing both
non-washed and washed
samples over a time span.
Rapid screening evaluating
(qualitatively) the presence of
antimicrobial (both
antibacterial and antifungal)
activity in or on the carpet face
fiber or incorporated into the
backing structure of the carpet
(or both). The method
simulates actual use
conditions that may occur on
carpets (for example, food and
beverage spills, soiling from
foot traffic, prolonged moisture
exposure) and provides a
means to screen for activity
and durability of an
antimicrobial treatment under
conditions of organic loading.
This test method provides for
the simultaneous assessment
of multiple carpet components
69
TYPE OF
REFERENCE
SOURCE
Own searches
REFERE
NCE
TITLE
PT

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
for antimicrobial activity.
Carpets may be cleaned prior
to testing in order to assess
the durability of the
antimicrobial effect. The
method can assess the
durability of the antimicrobial
treatments on new carpets,
and on those repeatedly
shampooed or exposed to inuse conditions.
Half strength (nutrient and
agar) tryptic soy agar is used
as the inoculum vehicle for
bacteria and half strength
potato dextrose agar as the
inoculum vehicle for mold
conidia. Use of half strength
agars may reduce undue
neutralization of an
antimicrobial due to excessive
organic load.
This test method
simultaneously evaluates (both
visual and stereo-microscopic)
antimicrobial activity both at
the fiber layer and at the
primary backing layer of
carpet.
TYPE OF
REFERENCE
SOURCE
ASTM
WK16397
Revision of E2180-01
for Determining the
In Polymeric or
Evaluates (quantitatively) the

antimicrobial effectiveness of
agents incorporated or bound
into or onto mainly flat (two
dimensional) hydrophobic or
polymeric surfaces. The
method focuses primarily on
assessing antibacterial activity;
however, other
microorganisms such as yeast
and fungal conidia may be
tested using this method.This
method can confirm the
presence of antimicrobial
activity in plastics or
hydrophobic surfaces. The
vehicle for the inoculum is an
agar slurry which reduces the
surface tension of the saline
formation of a "pseudobiofilm," providing more even
Manufacturer
(updated),
applied to
textiles.
70
REFERE
NCE
TITLE
PT
ASTM
WK4757

for the Assessment of
In Carpets; SeededAgar Overlay Screen

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
contact of the inoculum with
the test surface.
The method allows
determination of quantitative
differences in antimicrobial
activity between untreated
plastics or polymers and those
with bound or incorporated low
water-soluble antimicrobial
agents. Comparisons between
the numbers of survivors on
preservative-treated and
control hydrophobic surfaces
may also be made. The
procedure also permits
determination of "shelf-life" or
long term stability of an
antimicrobial treatment which
may be achieved through
testing both non-washed and
washed samples over a time
span.
Designed to evaluate
(qualitatively) the presence
and effectiveness of
antimicrobial preservatives in
or on carpets. This method can
be used to evaluate both the
antibacterial and antifungal
activity
This method is useful for
assessing the durability of the
antimicrobial treatments
because it can be performed
on both new carpets and those
that have been repeatedly
shampooed or exposed to inuse conditions.
This method utilizes either
tryptic soy agar as the
inoculum vehicle for bacteria
or potato dextrose agar as the
inoculum vehicle for mold
conidia. . Full compliment
agars can be used to mimic
high soil or organic loads on
carpets or partial nutrient
complement agars can be
used to mimic lightly soiled
conditions on carpet. This
method allows for the
simultaneous evaluation (both
71
TYPE OF
REFERENCE
SOURCE
Own searches
REFERE
NCE
BS
6085:199
2
EN ISO
117211:2001
EN ISO
117212:2003
EN ISO
20645:20
04
EN ISO
20743:20
07
JIS L
1902:
2002
JIS Z
2801:
2000
JIS Z
TITLE
PT
resistance of textiles
to microbiological
deterioration
Textiles Determination of the
resistance of cellulosecontaining textiles to
micro-organisms - Soil
burial test - Part 1:
Assessment of rotretardant finishing
(ISO 11721-1:2001)
Textiles Determination of the
resistance of cellulosecontaining textiles to
micro-organisms - Soil
burial test - Part 2:
Identification of longterm resistance of a rot
retardant finish (ISO
11721:2003)
Textile fabrics Determination of
antibacterial activity Agar diffusion plate
test (ISO 20645:2004)
Textiles Determination of
antibacterial activity of
antibacterial finished
products (ISO
20743:2007)
Testing for
antibacterial activity
and efficacy
on textile products
Manufacturer
Manufacturer
Own searches
Manufacturer
Antimicrobial Products
Test for antimicrobial
activity
and efficacy
Methods of test for

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
visual and stereo-microscopic)
of antimicrobial activity both at
carpet.
TYPE OF
REFERENCE
SOURCE
Applied to textiles
Manufacturer
Reference material: non

treated polyester
Laboratory
Antimicrobial activity.
Reference material: e.g. non
treated polyester.
Test institute
Applied to textiles
Manufacturer
Manufacturer
Test institute
Applied to textiles
72
Manufacturer
REFERE
NCE
TITLE
2911:
1992 (PT
9)
NF X41513
August
1961
fungus resistance
NF X41515
March
1962
NF XP
G39-010
May 2000
OECD
(ENV/JM/
BCID(200
7)5)
SN 195
920 1994
SN 195
921 1994
SN 195
924 1983
ASTM
WK8681
Protection of plastics.
Part 1. Testing method
for resistance of
ingredients to
microorganisms.
Protection of the
plastic materials - Part
3: Test method of the
resistance of materials
and apparatus to
microorganisms
Properties of textiles Textiles and polymeric
surfaces having
antibacterial properties
- Characterisation and
measurement of
Guidance document
on the evaluation of
the efficacy of
antimicrobial treated
articles with claims for
external effects
Antibacterial Activity,
Agar Diffusion Plate
Test
Antimycotic Activity
Test
antibacterial activity,
germ count method
for Resistance to Mold
Growth on Interior
Coated Building
Products in an
Environmental
Chamber
PT

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
TM II05
TM II05
Manufacturer
Own searches
Applied to textiles
Manufacturer
Applied to textiles
Manufacturer
Applied to textiles
Manufacturer
10
This test method can be used

to evaluate the comparative
resistance of coated building
products to accelerated mold
growth. Performance at a
certain rating does not imply
any specific period of time for
a mold free surface. However,
a better rated panel would be
expected to perform better in
actual end use.
An environmental chamber
and the conditions of operation
to evaluate in a 4-week period
Own searches
73
REFERE
NCE
TITLE
PT
ASTM
WK14960
Test Method for

for the Resistance of
Mold Growth on
Uncoated Interior
Building Products in an
Environmental
Chamber.
10
BS 3900
Methods of test for

paints. Part G6.
Assessment of
resistance to fungal
growth. BSI London,
UK
10

wood-based products Assessment of the
effectiveness of a
masonry fungicide to
prevent growth into
wood of Dry Rot
Serpula lacrymans
(Schumacher ex Fries)
S.F. Gray - Laboratory
method
Update on work to
validate efficacy
methods for
antimicrobials used on
hard surfaces
Determining the
10
ENV
12404:19
97
OECD
(ENV/JM/
BCID(200
7)3)
SM022

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
the relative resistance of
coated building products to
surface mold growth in a
controlled interior environment
is described. The apparatus is
interested party.
This test method can be used
to evaluate the comparative
resistance of uncoated building
products to accelerated mold
growth. The apparatus
(environmental chamber) is
interested party.
4-week evaluation period.
Relative resistance of
uncoated interior building
products to surface mold
growth in a controlled interior
environment is evaluated, but
performance at a certain rating
does not imply any specific
period of time for a mold free
surface.
Provides only a methodology
for production of a test surface
for exposure by inoculation
with mold growth. The test
therefore has to be modified to
be used as a test method for
assessing interior surface
biocides.
Simulated use test
TYPE OF
REFERENCE
SOURCE
Own searches
TNsG Prod Eval

UK guidelines
TNsG Prod Eval

TM II05 (Fr)
UK guidelines
10
10
Own searches
Resistance of masonry
74
Manufacturer
REFERE
NCE
TITLE
PT
(in
house)
growth
SM022a
(in
house)
Determining the
growth
10
SM023
(in
house)
Determining the
resistance to algal
growth
10
ASTM
E645-07

for Efficacy of
Microbicides Used in
Cooling Water
Systems
11

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
coatings to fungal growth.
Test substrate: Masonry
coatings on paper
Test germ: Aspergillus niger
and Penicillum funiculosum
"Sufficiently finished against
fungal growth" if max 1%
growth.
coatings on paper
Test germ: Aspergillus niger
and Penicillum funiculosum
"Sufficiently finished against
fungal growth" if max 1%
growth.
coatings to algal attack.
Test substrate: masonry
coatings on paper.
Test germ: Scenedesmus
vacuolatus.
No algal growth on the test
pieces after 2 weeks:
"Effectively protected against
algal growth"
Efficacy of microbicides
(algicides, bactericides, and
fungicides), evaluated using
simulated or real cooling tower
water against (1) microbes
from cooling water, (2)
microbes in microbiological
deposits (biofilms) from
operating cooling systems, or
(3) microorganisms known to
contaminate cooling water
systems, or a combination
thereof. Choice of enumerating
method may vary, e g pour
plate, spread plate, MPN
techniques etc. Test
concentrations also vary but
are usually between 1 and 50
mg/l. Exposure time varies
with mode of action but
includes 3h contact time. For
bacteria 48 h incubation, fungi
75
TYPE OF
REFERENCE
SOURCE
Manufacturer
Manufacturer
Manufacturer
REFERE
NCE
TITLE
PT
Not
available
Field trial in a Plant

recirculating cooling
system
11

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
48h-7d, for algae 5-14d.
Even if using cooling water
and deposits/biofilm obtained
from the field, laboratory
results may not be totally
predictive of microbicidal
effectiveness in the field (due
to e g variability in
environmental factors). If
solvents are added, a solvent
control is included. If initial
count in cooling water control
samples are <10E5 bact/ml (or
<10E3 CFU/ml fungi or algae),
the test is invalid. In addition,
the viable counts of fungi/algae
in the control must at least be
equal to the numbers at time 0.
The untreated control should
show a stable population with
no more than 0.5 log increase
or decrease in growth during
the test perod. Results are
expressed as Log reduction in
number of microorganisms at
each biocide concentration:
log (initial count of
microorganisms of the control
sample) log (number of
microorganisms detected at a
given biocide concentration
after a specific contact time). If
1 log reduction: corresponds to
90% kill, if 2 log reduction
corresponds to 99% kill, 3 log
reduction to 99.9% kill.
The antimicrobial effectiveness
of biocide was determined by
monitoring chemical and
physical cooling water
characteristics, microbiological
parameters such as viable
counts of bacteria, fungi and
algae, and operational and
engineering parameters of the
tested cooling system
Analysis methods used for
microbial counting were from:
Standard methods for the
Waste Water (APHA 1992),
and in-house Laboratory
76
TYPE OF
REFERENCE
SOURCE
Manufacturer
REFERE
NCE
TITLE
PT
SM019
(in
house)
S&M Boko test
11
ASTM
E723-07

for Efficacy of
Antimicrobials as
Preservatives for
Aqueous-Based
Products Used in the
Paper Industry
(Bacterial Spoilage)
11 or
12?
ASTM
E87500(2005)

for Efficacy of Fungal
Control Agents as
Preservatives for
Aqueous-Based
Paper Industry
11 or
12?

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
SOPs. Efficacy was
determined by a reduction in
microbial counts
Preservation of water diluted
coolants.
Test germ: bacteria, moulds,
Yeasts
4 evaluation levels. 12 cycles.
Laboratory test method is used
to determine the efficacy of an
antimicrobial for preventing
bacterial spoilage of in-process
pigment suspensions, dye
solutions, pulp slurries, starch
solutions, polymers, sizing
agents, latex emulsions, and
other aqueous-based materials
used in the paper industry from
bacterial spoilage. Test
organisms may vary but
should lie above 10E6 CFU/ml.
Bacterial numbers in the
sample are determined at
variable time periods and
compared to a control with no
bactericide. Material to be
preserved is used as
substrate, but for some
materials nutrients may be
added. Results are expressed
as % kill: [(control plate counttest plate count)/ control plate
count]x100 at each sampling
time.
Laboratory method that
determines if a fungal control
agent is effective to preserve
solutions, pulp slurries, starch
agents, latex emulsions, and
other specific aqueous-based
materials to prevent spoilage
of in-process aqueous-based
products used in the paper
industry. Will also prevent
spore germination. Plates are
77
TYPE OF
REFERENCE
SOURCE
Manufacturer
Communication
with UK (HSE)
Communication
with UK (HSE)
REFERE
NCE
TITLE
PT
ASTM
WK
17314
Test Method for

Evaluating the Efficacy
of a Liquid
Microbiocide against
Biofilm Bacteria
12
ASTM
E142700e1
Standard Guide for

Selecting Test
Methods to Determine
the Effectiveness of
12

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
incubated for 7d or until control
plates shos sufficient growth
for rating. The exposure time
should correspond to the real
time desired, often 6w.
Separate evaluations should
be made on a representative
type for each specific class of
product to be preserved.
Growth is determined by
visible signs of deterioration in
the sample and by obtaining
fungal numbers and comparing
them to a sample without
fungal control agents. A proper
level of fungal control agent
prevents deterioration and
reduces and keeps organisms
to an acceptable level in the
test material, which is
determined by the tester or
user. Often a scale from 0 to 4
(heavy growth).
A procedure for testing the
efficacy of a liquid microbicide
against biofilm grown on
coupons removed from biofilm
reactors standardized to grow
a repeatable biofilm, such as
the CDC Biofilm Reactor
(ASTM Method E 2562) or
Rotating Disk Biofilm Reactor
(ASTM Method E 2196). The
preparation of the liquid
microbicide and exposure time
are completed according to
manufacturers instructions for
use. The liquid microbicide is
tested at room temperature
under static conditions. Biofilm
population density is recorded
as log10 colony forming units
per surface area. Efficacy is
determined by calculating the
log reduction in viable cells.
The microbicide is tested at its
use dilution for the
recommended time.
The purpose of this guide is to
inform the investigator of
methods that can be used for
biofilm formation and
78
TYPE OF
REFERENCE
SOURCE
Own searches
Own searches
REFERE
NCE
TITLE
PT
and Other Chemicals
for the Prevention,
Inactivation and
Removal of Biofilm
ASTM
E183907
ASTM
WK14214
EEC:
1988
Report
EPS
1/RM/25
Environm
ent
Canada.
1992
ASTM
E227503e1
(replaces
D3946
and
E686)
Standard test method

for efficacy of
slimicides for the
paper industry
bacterial and fungal
slime
12
Revision of E183996(2002) Standard

Test Method for
Efficacy of Slimicides
for the Paper Industry-Bacterial and Fungal
Slime
12
12
Algal inhibition test
Biological Test
Method: Growth
Inhibition Test Using
the Freshwater Alga
Selenastrum
capriconutum
Evaluating WaterMiscible Metalworking
Fluid Bioresistance
and Antimicrobial
Pesticide Performance
12

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
measurement, allowing
development of test
procedures for determining the
effectiveness of chemical
treatments for prevention,
inactivation, and removal of
unwanted biofilm. This guide is
not an exhaustive survey of
biofilm methods.
A procedure to evaluate the
efficacy of slimicides for the
control of bacterial and fungal
slimes in paper mill systems
and their counterparts. This
test method is run in acid,
alkaline, or acid and alkaline
conditions to determine the
efficacy of the slime control
agent. The test conditions may
be modified to reflect intended
use patterns in typical paper
mill systems, including use of
actual paper mill furnish.
Efficacy based on a %
reduction on CFU.
Efficacy of slimicides for the
control of bacterial and fungal
slimes in paper mill systems
and their counterparts.
TYPE OF
REFERENCE
SOURCE
Used in the process of oil

recovery
Efficacy test against Alga:
Selenastrum capriconutum
Communication
with UK (HSE)
Communication
with UK (HSE)
Communication
with UK (HSE)
Manufacturer
Own searches

recovery
13
Laboratory procedures for

rating the relative inherent
bioresistance of water-miscible
metalworking fluids, the
bioresistance attributable to
augmentation with
antimicrobial pesticides or
both, for determining the need
for microbicide addition prior to
or during fluid use in
metalworking systems and for
79
TNsG on Prod
Eval
REFERE
NCE
TITLE
PT
ASTM
E97991(2004)

for Evaluation of
as Preservatives for
Invert Emulsion and
Other Water
Containing Hydraulic
Fluids
New Standard Test
Method for
Determining
13
ASTM
WK8252
13

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
evaluating microbicide
performance.
Relative bioresistance is
determined by challenging
metalworking fluids with a
biological inoculum that may
either be characterized
(comprised of one or more
known biological cultures) or
uncharacterized (comprised of
biologically contaminated
metalworking fluid or one or
more unidentified isolates from
deteriorated metalworking
fluid). Challenged fluid
bioresistance is defined in
terms of resistance to biomass
increase, viable cell recovery
increase, chemical property
change, physical property
change or some combination
thereof.
This practice is applicable to
antimicrobial agents that are
incorporated into either the
metalworking fluid concentrate
or end-use dilution. It is also
applicable to metalworking
fluids that are formulated using
non-microbicidal, inherently
bioresistant components.
The results of tests completed
in accordance with this
practice should be used only to
compare the relative
performance of products or
microbicide treatments
included in a test series.
Results should not be
construed as predicting actual
field performance.
TYPE OF
REFERENCE
SOURCE
TNsG on Prod
Eval
Determines the relative

bioresistance of aqueous
metalworking fluids towards
80
Own searches
REFERE
NCE
TITLE
PT
Resistance of
Aqueous Metalworking
Fluids towards NonTuberculous,
Environmental
Mycobacteria
IBRG
(draft
MWF)
(1993)
Not
available
Rawlinso
n and
Shennan,
1987.
A Standardized
Screening Method for
Determining the
Bioresistance of and
Evaluating Biocides in
Aqueous Metal
Working Fluids
Challenge Testing in
Metal Working
Emulsion (in-house
laboratory study)
13
A recirculating test rig

for the investigation of
metal-working fluid
spoilage. In Industrial
microbiological testing.
Edited by Hopton and
13
13

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
non-tuberculous (NTM), rapidly
growing( RGM), environmental
mycobacteria by challenging
them with a mycobacterial
inoculum isolated from actual
spoiled metalworking fluid field
samples from the user/s site.
In order to simulate field
conditions, another challenge
inoculum consisting of a
mixture of common
metalworking fluid spoilage
microorganisms originating
from actual MWF field samples
is also used.
TYPE OF
REFERENCE
SOURCE
TNsG on Prod
Eval
The purpose of the method

was to determine the efficacy
of preservatives against
contamination in Metal
Working Fluids (MWF).
inoculating once a week during
10 weeks mixed bacteria and
optionally mixed fungi into
water based MWF emulsions
containing various amounts of
the test substance diluted in
3% synthetic oil. After each
week a sample of the emulsion
was plated. After plate
compared to the control The
criteria for measuring good
microbial count of <10 cfu/ml
after 10 inoculations for all
microorganisms.
Manufacturer
TNsG on Prod
Eval
81
REFERE
NCE
RENAUL
T
D551721
(1987)
SABS
14351987
UK MOD
91-70
issue
(1990)
BBA 9 3.1
TITLE
Hill, Blackwell
Scientific Publications,
Oxford. ISBN 0 632
01793 7. pp 227-231
Evaluation of the
Biostability of Aqueous
Metal Working Fluids
PT

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
13
TNsG on Prod
Eval
South African standard

specification for
biocides for use in
emulsions of aqueous
metal working fluid and
aqueous hydraulic
fluid.
Cutting fluid, soluble,
biostable joint service
designation ZX-9
13
TNsG on Prod
Eval
13
TNsG on Prod
Eval
Richtlinie fr die
Prfung von
Nagetierbekmpfungs
mitteln gegen
Hausmase
14
TNsG on Prod
Eval
UK guidelines
Manufacturer
BBA 9 3.2
Richtlinie fr die
Prfung von
Nagetierbekmpfungs
mitteln gegen
Wanderratten
14
TNsG on Prod
Eval
UK guidelines
Manufacturer
CEB
(1981)
Laboratory test
method to evaluate the
efficacy of rodenticidal
products in rats
14
CEB
(1981)
Trial method to
evaluate the efficacy of
rodenticidal products
against rats under
practical conditions
Standard Norway Rat
and Roof Rat
Anticoagulant Liquid
Bait Laboratory Test
Method
14
EPA/OPP
Protocol
Number:
1.201
14
Laboratory test method
TMII Fr
TMII Fr
Anticoagulant rodenticide test

against Norway rat/Roof rat
applied as liquid bait
TNsG on Prod
Eval
NL guidance
UK guidelines
EPA/OPP
Standard House
14
82
TNsG on Prod
REFERE
NCE
TITLE
Protocol
Number:
1.202
Mouse Anticoagulant
Liquid Bait Laboratory
Test Method
PT

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
against House mouse applied
as liquid bait
TYPE OF
REFERENCE
SOURCE
Eval
NL guidance
UK guidelines
EPA/OPP
Protocol
Number:
1.203
Standard Norway Rat

and Roof Rat
Anticoagulant Dry Bait
Laboratory Test
Method
14

applied as dry bait
TNsG on Prod
Eval
NL guidance
UK guidelines
EPA/OPP
Protocol
Number:
1.204
Standard House
Mouse Anticoagulant
Dry Bait Laboratory
Test Method
14

as Dry bait
TNsG on Prod
Eval
NL guidance
UK guidelines
EPA/OPP
Protocol
Number:
1.205
EPA/OPP
Protocol
Number:
1.207
Standard Norway
Rat/Roof Rat
Anticoagulant Tracking
Powder Efficacy
Laboratory Test
Method
14
Standard Norway
Rat/Roof Rat Acute
Test Method
14

applied as Tracking powder
TNsG on Prod
Eval
NL guidance
UK guidelines
Acute rodenticide test against

Norway rat/Roof rat applied as
Liquid bait
TNsG on Prod
Eval
NL guidance
UK guidelines
EPA/OPP
Protocol
Number:
1.208
Standard House
Mouse Acute Liquid
Method
14

House mouse applied as
Liquid bait
TNsG on Prod
Eval
NL guidance
UK guidelines
EPA/OPP
Protocol
Number:
1.209
Standard Norway
Rat/Roof Rat Acute
Dry Bait Laboratory
Test Method
14

Dry bait
TNsG on Prod
Eval
NL guidance
UK guidelines
EPA/OPP
Protocol
Number:
1.210
Standard House
Mouse Acute Dry Bait
Laboratory Test
Method
14

House mouse applied as Dry
bait
TNsG on Prod
Eval
NL guidance
83
REFERE
NCE
TITLE
PT

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
UK guidelines
EPA/OPP
Protocol
Number:
1.211
Standard Norway
Rat/Roof Rat Acute
Tracking Powder
Efficacy Laboratory
Test Method
14

Tracking powder
TNsG on Prod
Eval
NL guidance
UK guidelines
EPA/OPP
Protocol
Number:
1.212
Standard House
Mouse Anticoagulant
Tracking Powder
Efficacy Laboratory
Test Method
14

as Tracking powder
TNsG on Prod
Eval
NL guidance
UK guidelines
EPA/OPP
Protocol
Number:
1.213
EPA/OPP
Protocol
Number:
1.214
Standard Norway
Rat/Roof Rat
Anticoagulant Wax
Block and Wax Pellet
Laboratory Test
Method
14
Standard House
Mouse Anticoagulant
Wax Block and Wax
Pellet Laboratory Test
Method
14

applied as Wax block and wax
pellet
TNsG on Prod
Eval
NL guidance
UK guidelines

as Wax block and wax pellet
TNsG on Prod
Eval
NL guidance
UK guidelines
EPA/OPP
Protocol
Number:
1.217
EPA/OPP
Protocol
Number:
1.218
Standard Norway Rat

and Roof Rat
Anticoagulant
Placepack Dry Bait
Laboratory Test
Method
14
Standard House
Mouse Anticoagulant
Placepack Penetration
Laboratory Test
Method
14

applied as Placepark dry bait
TNsG on Prod
Eval
NL guidance
UK guidelines

as Placepark penetration
TNsG on Prod
Eval
NL guidance
UK guidelines
EPA/OPP
Protocol
Number:
1.219
Standard Norway Rat

and Roof Rat Acute
Laboratory Test
Method
14

Placepark penetration
TNsG on Prod
Eval
NL guidance
UK guidelines
84
REFERE
NCE
TITLE
PT
EPA/OPP
Protocol
Number:
1.220
Standard House
Mouse Acute
Placepack Dry Bait
Laboratory Test
Method
14

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
Placepark dry bait
TYPE OF
REFERENCE
SOURCE
TNsG on Prod
Eval
NL guidance
UK guidelines
EPA/OPP
Protocol
Number:
1.221
EPA/OPP
Protocol
Number:
1.222
Proposed Norway Rat

Anticoagulant
Technical and
Concentrated Dry Bait
Laboratory Test
Method
14
Proposed Norway Rat

Acute Technical and
Laboratory Test
Method
14

against Norway rat applied as
Technical and concentrated
dry bait
TNsG on Prod
Eval
NL guidance
UK guidelines

Norway rat applied as
dry bait
TNsG on Prod
Eval
NL guidance
UK guidelines
EPA/OPP
Protocol
Number:
1.225
EPA/OPP
Protocol
Number:
1.226
EPA/OPP
Protocol
Number:
1.227
Proposed House
Mouse Anticoagulant
Technical and
Laboratory Test
Method
14
Proposed House
Mouse Acute
Technical and
Laboratory Test
Method
14
Proposed House
Mouse Acute Tracking
Powder Efficacy
Laboratory Test
Method
14

against House mouse, applied
as Technical and
concentrated dry bait
TNsG on Prod
Eval
NL guidance
UK guidelines

dry bait
TNsG on Prod
Eval
NL guidance
UK guidelines

Tracking powder
TNsG on Prod
Eval
NL guidance
UK guidelines
14
EPPO
PP
1/169(2
EPPO
PP
1/114(2)
EPPO
PP
Field rodents
(Microtus, Arvicola)
Field tests against
synanthropic rodents
(Mus musculus, Rattus
norvegicus, R. rattus)
Laboratory and field
tests for the evaluation
NL guidance
TM II05 (Fr)
14
NL guidance
TM II05 (Fr)
14
NL guidance
85
REFERE
NCE
TITLE
1/97(2)
of rodenticidal dusts
evaluation of the
toxicity and
acceptability of
rodenticides and
rodenticide
preparations
EPPO
PP
1/113(2)
EPPO
PP
1/197(1)
EPPO
PP
1/200(1)
EPPO
PP
1/199(1)
EPPO
PP
1/198(1)
EPPO
(1982)
EPPO
(1982)
EPPO
(1986)
US EPA
96-5
US EPA
96-7
ASTM
D413184
PT

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
TM II05 (Fr)
NL guidance
14
TM II05 (Fr)
Test institute
14
Non-target effects of
rodenticides
Rodent repellents
against debarking of
trees
Rodent seed
repellents
Testing rodents for
resistance to
anticoagulant
rodenticides
Guidelines for the
biological evaluation of
rodenticides N1.
evaluation of the
toxicity and
acceptability of
rodenticides and
rodenticide
preparations
Guidelines for the
rodenticides. Field
tests against
norvegicus, rattus
rattus)
Guidelines for the
rodenticides.
of rodenticidal dusts.
NL guidance
14
Own search
14
Own search
14
NL guidance
TM II05 (Fr)
14
TNsG on Prod
Eval
UK guidelines
Manufacturer
14
TNsG on Prod
Eval
UK guidelines
Manufacturer
14
TNsG on Prod
Eval
UK guidelines
15
Avian Toxicants
Avian Frightening
Agents
sampling fish with
Rotenone
15
17
Adaptable to both lotic and

lentic situations in littoral and
limnetic areas.
86
TNsG on Prod
Eval
TNsG on Prod
Eval
Communication
with UK (HSE)
REFERE
NCE
TITLE
PT

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
Standard guide for

conducting acute
toxicity tests on test
materials with fishes,
macroinvertebrates
and amphibians
Standard guide for
conducting early lifestage toxicity test with
fishes
17
2 to 8-day exposures,
depending on the species.
Static, renewal, or flow-through
procedures.
Usually reported as LC50 or
EC50.
A flow through test, beginning
before hatch and ending after
hatch. 28 to 120-day
(depending on species)
continuous exposure.
Communication
with UK (HSE)
(2005)
ASTM
E729-96
(2007)
ASTM
WK6845
[revision
of E
124198 (2004)
]
EPA 725
EPA 727
OECD
203
(1992)
OECD
204
(1984)
US EPA
72-1
US EPA
96-2
810.3000
(1999)
17
17
Life-cycle test of fish
Simulated or actual
field testing for aquatic
organisms
Fish, Acute toxicity test

Fish, Prolonged
toxicity test: 14-day
study
Acute toxicity test for
freshwater fish
Communication
with UK (HSE)
Communication
with UK (HSE)
17
17
Communication
with UK (HSE)
17
Communication
with UK (HSE)
17
Communication
with UK (HSE)
Communication
with UK (HSE)
Manufacturer
17
Fish control agents
General
Considerations for
Efficacy of Invertebrate
Control Agents
Communication
with UK (HSE)
18
General guide
UK guidelines
AATCC
194-2006
ASTM
E151799(2006)
Assesment of the AntiHouse Dust Mite

Properties
of Textiles under LongTerm Test Conditions
for Determining the
Effectiveness of
Liquid, Gel, Cream, or
Shampoo Insecticides
Against Human Louse
OVA
18
Applied to textiles
Manufacturer
18
Determines the effectiveness

of ovicidal materials in liquid,
gel, cream, or shampoo form
against the ova (that is, eggs
or nits) of the human louse,
Pediculus humanus.
This test method consists of
five replicates for a statistical
Own searches
87
REFERE
NCE
TITLE
PT
ASTM
E65291(2003)

for Nonresidual Liquid
Household
Insecticides Against
Flying Insects
18
ASTM
E65391(2003)

for Effectiveness of
Aerosol and
Pressurized Space
Spray Insecticides
Against Flying Insects
18

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
comparison of formulations.
efficiency of household and
industrial-use, contact
insecticides dissolved in base
oils and applied in spray
formulations. It is developed to
test insecticides against
house flies (Musca domestica,
L), but test data may also be
adequate to support label
claims for the use of the
products against mosquitoes,
gnats, flying moths, wasps,
and certain other small flying
insects. Not designed to
measure the residual action of
the spray formulation.
The test determines the
relative efficacy of aerosol and
pressurized space spray
insecticide formulations
against house flies (Musca
domestica, L) strains and, with
modifications in dosage, other
flying insects. Test data
obtained by this test method
may also be adequate to
support label claims for the
use of the product against
mosquitoes, gnats, flying
moths, wasps, and certain
other small flying insects.
This test method is not
designed to measure the
residual activity.
The test may be conducted
using approximately 100
house flies per test (small
group) or 500 flies per test
(large group). Selected
reference standards are the
Official Test Aerosol II (OTA II)
for oil based aerosol products
and Tentative Official Aqueous
Pressurized Spray (TOAPS)
for water based aerosol
products. Aerosol test
knockdowns: % down of total
flies at 5, 10, 15 minutes after
application. Aerosol test knock
down mortality: dead knocked
88
TYPE OF
REFERENCE
SOURCE
Own searches
UK guidelines
REFERE
NCE
TITLE
PT
ASTM
E65496(2003)

Aerosol and
Pressurized Spray
Cockroaches
18
ASTM
E938-05

Liquid, Gel, or Cream
18

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
down x100/total flies. These
numbers should on average be
equal to, greater than or no
more than 5% points below the
corresponding numbers of the
reference in order to meet the
standard. No statement on
precision or bias, only whether
conformance to criteria for
success specified in the
procedure.
Test of insecticides against
crawling insects: cockroaches
efficiency of aerosol and
pressurised spray formulations
against cockroaches, but test
data by this test method may
also be adequate to support
claims for use of the product to
control the exposed or
accessible stages of silverfish,
ants, centipedes, spiders, and
certain stored product pests.
Applied as direct sprays for 30
s. on last instar nymphs.
Observation period: 48h. The
test is not designed to
measure the residual action.
Ten groups with 20 organisms
in each. The test is run in
conjunction with the Official
Test Aerosol II (OTA II) (or
Tentative Official Aqueous
Pressurized Spray (TOAPS)
as the standard basis of
comparison. The mortality after
24h should be between 50 and
75% when testing with the
OTA. The test specimens meet
the standard if average %
dead and moribound is equal
to, above or within 10% points
less than average % dead of
the OTA series after 48h.
Precision or bias is not
specified, only states whether
conforms to efficacy criteria.
crawling insects: human louse.
Only gels or creams that
liquefy at 32C (90F) can be
89
TYPE OF
REFERENCE
SOURCE
UK guidelines
Own searches
REFERE
NCE
TITLE
PT
Adult Human Lice
BS 41721:1999
BS 41722:1999
CEB 107
(1985)
CEB 135
(1987)
CEB
135bis
(1996)
CEB 159
(1992)
CEB 196
(1997)
Hand-held pressurized
aerosol dispensers
against houseflies.
Specification for
insecticidal
performance
18
aerosol dispensers
against houseflies.
Method for
determination of
insecticidal
performance
Trial method to
evaluate the efficacity
of insecticidal products
for the control of stable
flies in premises for
the rearing of domestic
animals under
Trial method to
insecticidal or miticidal
products for
treatments of storage
premises of products
for animals or plants
Laboratory test
method to evaluate the
efficacy of insecticidal
products in premises
for the storage,
industrial processing
and sale of products
from animals or plants
Trial method to
insecticidal products
for the control of
cockroaches in
buildings under
Trial method to
insecticidal bait
products against
common species
18

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
tested).
Five replicates, for a statistical
comparison of formulations.
TYPE OF
REFERENCE
SOURCE
TNsG on Prod
Eval
TM II05 (Fr)
UK guidelines
TNsG on Prod
Eval
TM II05 (Fr)
UK guidelines
18
18
Surface treatments of storage

premises of products
TM II05 (Fr)
18
Space treatments
TM II05 (Fr)
18
TM II05 (Fr)
18
TM II05 (Fr)
90
REFERE
NCE
TITLE
PT
CEB 213
(1999)
Trial method to
a fumigant for insect
control in premises for
the storage,
processing and
production of food
Trial method to
fumigants for insect
control in stored
products
Laboratory testing of
plant protection
products against insect
and mite pests of
stored plant products
18
TM II05 (Fr)
18
TM II05 (Fr)
18
UK guidelines
18
TM II05 (Fr)
CEB 224
(2001)
EPPO
PP
1/204(1)
EPPO
PP
1/202(1)
EPPO
Bulletin,
15 Pages
1-119,
Paris
(1983)
EPPO,
Paris
(1982)
ISO
3998:197
7
MS 1004
part 1
(2002)
MS 1004
part 2
(2002)
Space and structural

treatments of store
rooms
The EPPO Conference
on Fumigation, Paris,
1983

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
UK guidelines
18
TNsG on Prod
Eval
UK guidelines
EPPO
Recommendations on
fumigation standards
(2nd Edition)
Textiles Determination of
resistance to certain
insect pests
Specification for
mosquito vapourising
mat: part 1: physical
and chemical
requirements (first
revision)
Specification for
mat: part 2: method for
18
18
TNsG on Prod
Eval
Applicable to all textiles

containing animal fibres in any
proportion. Conditioned
voracity control specimens and
test specimens of known mass
are placed in contact with
selected larvae for 14 days.
The loss in mass of all
specimens and the condition of
the test larvae are ascertained
to assess the resistance of
each test specimen.
UK guidelines
TNsG on Prod
Eval
TM II05 (Fr)
UK guidelines
18
Manufacturer
18
Manufacturer
91
REFERE
NCE
MS 1004
part 3
(2002)
MS 1004
part 4
(2002)
MS 1008
(1986)
MS 1255
(1992)
MS 1257
(1992)
MS 1364
(1994)
MS 1398
part 1
(1996)
MS 1398
part 2
(1996)
MS 1398
part 3
(1996)
MS 1497
(2000)
TITLE
evaluation of biological
efficacy - glass
chamber method (first
revision)
Specification for
efficacy - glass
cylinder method
Specification for
efficacy - peet grady
method
Method for
determination of
delivery rate of aerosol
dispenser
Specification for
household insecticidal
residual spray aerosol
Specification for
space spray aerosol
Method for
determination of
brimful capacity of
aerosol cans
Specification for
mosquito electric liquid
vaporizer: Part 1:
physical and chemical
requirement
Specification for
vapourizer: part 2:
method for evaluation
of biological efficacy glass chamber method
Specification for
vapourizer: part 3:
of biological efficacy glass cylinder method
Methods of biological
evaluation of the
efficacy of repellent bioassay method for
mosquito repellent on
PT

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
18
Manufacturer
18
Manufacturer
18
Manufacturer
18
Manufacturer
18
Manufacturer
18
Manufacturer
18
Manufacturer
18
Manufacturer
18
Manufacturer
18
Manufacturer
92
REFERE
NCE
MS 23
part 1
(1998)
MS 23
part 2
(1996)
MS 23
part 3
(1998)
NF G39011 April
2001
NF T72320
March
1977
NF T72321
March
1977
NF X41516
January
1980
Not
available
TITLE
human skin
Specification for
mosquito coils: Part 1:
requirements (third
revision)
Specification for
of biological efficacy glass chamber method
(first revision)
Specification for
of biological efficacy peet grady method
materials having
antiacarien properties Characterisation and
measurement of
antiacarien activity
Insecticides for flying
insects. Insecticide
distributed under
pressure ("aerosol"
type). Determination of
the efficiency rating.
insects. Permanent
insecticide distributor.
efficiency rating and
the regularity rating.
Protection of textiles.
Protection against
certain insect pests.
Methods of testing.
Field Evaluation of in a
Termite Control Baiting
System
PT

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
18
Manufacturer
18
Manufacturer
18
Manufacturer
18
Manufacturer
TM II05 (Fr)
18
TNsG on Prod
Eval
TM II05 (Fr)
UK guidelines
18
TM II05 (Fr)
18
TM II05 (Fr)
18
Structures in this study were

supplied by cooperating pest
control companies. A number
of the structures had been
identified as "problem houses"
by the pest control operator.
Stations were inspected at
approximately monthly (when
active), and bi-monthly (when
not active) intervals for termite
activity, with the condition of
93
Manufacturer
REFERE
NCE
TITLE
PT
Not
available
In house Laboratory
Study of the Efficacy
against Reticulitennes
fIavipes
18
Not
available
Insecticidal activity of
an oil based aerosol
against German
cockroach, Blattella
germanica (in-house
laboratory study)
18
Not
Insecticidal efficacy of
18

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
the station (active, inactive and
baited) being noted on an
inspection form. When activity
was found in a station, bait
was placed in the station.
The thin wood strips of the
baiting station were fan
sprayed with product solution
using a compressed air
sprayer. Product was mixed in
the acetone such that when
the solution was applied to the
strips, various concentrations
of product were imparted to
the wood strips .A tumble
technique was employed to
prevent uneven concentrations
of active ingredient in the
sawdust matrix. Efficacy was
measured by elimination of
termite infestation
Formulation, applied as a
residual spray, was assessed
for efficacy against
cockroaches (Blatta orientalis,
Blatella germanica and
Periplaneta americana) under
laboratory conditions. The
spray deposits were assessed
at intervals up to 24 weeks
post application. Efficacy of the
formulation at each interval, in
terms of knockdown and
mortality, was evaluated over a
period of 96 hours post
treatment application
Efficacy was measured when
all termites in a container had
died, the length of time of
consumption by the termites of
the treated sawdust was
recorded
10 German cockroaches
(Blattella germanica) were
released into a plastic
container within a glass
cylinder and the aerosol was
sprayed into the cylinder.
Knocked down insects were
counted at intervals up to 20
minutes.
6 adult American cockroaches
94
TYPE OF
REFERENCE
SOURCE
Manufacturer
Manufacturer
Manufacturer
REFERE
NCE
TITLE
PT
available

against American
cockroach, Periplaneta
Americana
Not
available
an oil-based aerosol
against Housefly,
Musca domestica.
18
Not
available
against mosquito,
Culex pipiens pallens
18
Not
available
Insecticide primarily for

the control of termites:
Laboratory Study
fIavipes
18

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
(Periplaneta Americana) were
released into plastic container
which was placed in the centre
at the bottom of the glass
cylinder and 1013mg of
aerosol was sprayed into the
cylinder and was then covered
by a glass lid. Knocked down
insects were counted at
intervals up to 20 minutes.
100 houseflies were released
into a Peet-Grady chamber
and aerosol was sprayed into
the chamber. Knocked down
50 mosquitoes (Culex pipiens
pallens) were released into a
Peet-Grady chamber and
aerosol was sprayed into the
chamber. Knocked down
The chamber was then
ventilated by an exhaust fan
and all insects were
transferred to a clean recovery
container with diet and water
within 20 minutes
product were impregnated in a
sawdust matrix readily
consumed by termites. Product
was incorporated into the
sawdust by placing each batch
of solution and one batch of
sawdust in the mixing bowl of
a Kitchenaid Model K5SS
mixer. One untreated batch of
sawdust served as a control.
Sterile sand and distilled water
was mixed to form a
moistened sand substrate to
sustain the termites as they
fed upon the samples.
Eight replications of each
concentration plus the control
were tested. Termite activity
was observed in each of the
containers for four days.
elimination of termite
95
TYPE OF
REFERENCE
SOURCE
Manufacturer
Manufacturer
Manufacturer
REFERE
NCE
TITLE
PT
Not
available
Laboratory studies to
assess products
applied as direct
sprays for efficacy
against a range of
crawling insects
18
OPPTS
810.3100
OPPTS
810.3200
Soil treatments for

imported fire ants
Livestock, poultry, furand wool-bearing
animal treatments
Treatments to control
pests of humans and
pets
18
Own searches
18
Own searches
18
UK guidelines
OPPTS
810.3400
Mosquito, black fly,

and biting midge (sand
fly) treatments
18
OPPTS
810.3500
Premises treatments
18
OPPTS
810.3300

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
infestation.
Product was applied as a
direct spray, was assessed for
efficacy against cockroaches
(Blatta orientalis, Blatella
germanica and Periplaneta
americana) under laboratory
conditions. Efficacy criteria
based on mortality and
knocked down insects

flying insects: Mosquito
Black Fly
Biting Midge (Sand Fly)
General guideline
TYPE OF
REFERENCE
SOURCE
Manufacturer
UK guidelines
Manufacturer
UK guidelines
OPPTS
810.3800
SABS
st
233 1
rev
SABS
303
SABS
332
SABS
458
SABS
576
Methods for efficacy

testing of termite baits
Pesticides: Biological
evaluation of mists and
fogs - first revision
Pesticides Rearing
and handling of the
human body louse
(Pediculus humanus
humanus L.) - first
revision
Pesticides Rearing
and handling of the
common clothes moth
(Tineola bisseliella
Hummel) - second
revision
Pesticides Rearing
and handling of the
German cockroach
(Blatella germanica
(L.)) - second revision
Pesticides Biological
evaluation of
insecticidal oil-based
18
Own searches
18
Manufacturer
18
Manufacturer
18
Manufacturer
18
Manufacturer
18
Manufacturer
96
REFERE
NCE
SABS
583
SABS
6136
(2003)
SABS
rd
689 3
ed (2002)
SABS
690
(DRAFT)
SABS
807
TITLE
space spray in lowpressurized

dispensers - first
revision
evaluation of the
contact efficacy of
liquid residual
insecticides - first
revision
evaluation of materials
that release an
insetticide upon
heating
evaluation of knockdown and killing
proprieties of liquid
and aerosol
formulation (al posto
di Standard methods
SABS Method 8689first revision)
Pesticides: biological
evaluation of the
proprerties of solid fly
baits - DRAFT
Methods for testing
insecticides against
flying and crawling
insects.
PT

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
18
Manufacturer
18
Manufacturer
18
Manufacturer
18
Manufacturer
18
TNsG on Prod
Eval
Manufacturer
UK guidelines
SABS
899
(1987)
US
AATCC
Technical
Manual
Method
24 (1992)
US
CSMA
Aerosol
Guide
7 th
Edition,
pages
129-134
Insecticidal space
spray in pressurized
dispensers
Test method for
textiles to determine
resistance to insects
(e.g. moths, carpet
beetles)
18
Test method for

aerosol space sprays
against flying insects
18
18
Manufacturer
Efficacy test against larvae
TNsG on Prod
Eval
UK guidelines

flying insects:
TNsG on Prod
Eval
UK guidelines
Manufacturer
97
REFERE
NCE
(1981)
US
CSMA
Aerosol
Guide
7 th
Edition,
pages
135-139
(1991)
Verwey &
Sosa,
2007
WHO/CD
S/WHOP
ES/GCD
PP/2003.
5
WHO/VB
C/75.593
(1981)
WHO/VB
C/81.212
(1981)
WHO/VB
C/81.805
TITLE
PT

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
Test method for

pressurised spray
products against
cockroaches
18

crawling insects: cockroaches
TNsG on Prod
Eval
Liquid Electric test

method
18
Space spray
application of
insecticides for vector
and public health pest
control a
practitioners guide
Instructions for
determining the
susceptibility or
resistance of
cockroaches to
insecticides
Instructions for
determining the
susceptibility or
resistance of mosquito
larvae to insect
development inhibitors
Instructions for
determining the
susceptibility or
resistance of adult
mosquitoes to
organochlorine,
18
UK guidelines
For testing pyrethroids (draft

method) and natural actives
(Pyrethrum extract) on
mosquitoues (knockdown).
Efficacy criteria: "effective
against mosquitoes for X
hours". Knockdown is
measured repeatedly for 2h
and mortality after 24h.
Control (no treatment)
knockdown: maximum 10%.
2-4 chamber replicates, 50
organisms in each. Mean and
Standard Deviations for each
time calculated as well as
KT50 and KT80 (Mean time to
50% and 80% knockdown
respectively).
Brief description of the main
types of space spray
equipment as well as the
operational guidelines for
space spray application of
insecticides.
18
Manufacturer
TM II05 (Fr)
TNsG on Prod
Eval
UK guidelines
18
TNsG on Prod
Eval
18
TNsG on Prod
Eval
UK guidelines
98
REFERE
NCE
WHO/VB
C/81.806
WHO/VB
C/81.807
(1981)
WHO/VB
C/81.808
(1981)
WHO/VB
C/81.809
(1981)
WHO/VB
C/81.810
(1981)
WHO/VB
C/81.811
(1981)
WHO/VB
C/81.812
(1981)
WHO/VB
C/81.813
(1981)
TITLE
organophosphate and
carbamate
insecticides, establishment of the
baseline.
Instructions for
determining the
susceptibility or
resistance of adult
mosquitoes to
organochlorine,
organophosphate and
carbamate insecticides
- diagnostic test
Instructions for
determining the
susceptibility or
larvae to insecticides
Instructions for
determining the
susceptibility or
resistance of body or
headlice to
insecticides
Instructions for
determining the
susceptibility or
resistance of adult
bed-bugs to
insecticides
Instructions for
determining the
susceptibility or
resistance of adult
blackflies, sandflies
and biting midges to
insecticides
Instructions for
determining the
susceptibility or
resistance of blackfly
Instructions for
determining the
susceptibility or
larvae to insect
Instructions for
determining the
susceptibility or
PT

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
18
TYPE OF
REFERENCE
SOURCE
TNsG on Prod
Eval
UK guidelines
18
TNsG on Prod
Eval
UK guidelines
18
TNsG on Prod
Eval
18
TNsG on Prod
Eval
UK guidelines
18
TNsG on Prod
Eval
UK guidelines
18
TNsG on Prod
Eval
UK guidelines
18
UK guidelines
18
TNsG on Prod
Eval
99
REFERE
NCE
WHO/VB
C/81.814
(1981)
WHO/VB
C/81.815
(1981)
EPPO
PP
1/203(1)
EPPO
PP
1/201(1)
ASTM
E93994(2006)
TITLE
resistance of
houseflies, tsetse flies,
stableflies, blowflies
etc. to insecticides
Instructions for
determining the
susceptibility or
resistance of adult
ticks to insecticides
Instructions for
determining the
susceptibility or
resistance of fleas to
insecticides
Admixture of plant
protection products to
to control insects and
mites
Fumigants to control
insect and mite pests
of stored plant
products
of Field Testing
Topical Applications of
Compounds as
Repellents for
Medically Important
and Pest Arthropods
(Including Insects,
Ticks, and Mites):
Mosquitoes
PT

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
UK guidelines
18
TNsG on Prod
Eval
UK guidelines
18
TNsG on Prod
Eval
UK guidelines
18 +
20
TM II05 (Fr)
18 +
20
TM II05 (Fr)
UK guidelines
19
ASTM
E95194(2006)
Standard Test
Methods for
Laboratory Testing of
Non-Commercial
Mosquito Repellent
Formulations On the
Skin
19
Dautel H,
Hilker M,
Kahl O,
Verwendung von
Dodecansure als
Zeckenrepellent
19
Evaluates the repellency of

promising compounds that
have undergone primary
laboratory studies and
approved for skin application
for secondary testing.
The method is designed for the
study of mosquito repellents,
but can be modified to
determine the repellency of
candidate compounds for other
flying insects that attack
humans.
Can be used to test the
efficacy of repellent
compounds that can be diluted
with ethanol, acetone etc. Both
biological effectiveness and
persistence of the repellent
can be assessed.
ED50 and ED95 are
determined for comparative
and practical purposes
respectively. Precision of the
test can be evaluated (confid
intervals).
Own search
Own search
Dossier
100
REFERE
NCE
TITLE
PT

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
Siems K,
2001.
Fradin &
Day, July
2002
N Engl J
Med vol
347 vol
13-18
(Patentschrift).
Comparative efficacy
of insect repellents
against mosquito bites
19
Human subjects: Arm in cage

studies (15 volunteers, 10
mosquitoues (Aedes aegypti)
in each cage. Endpoint:
elapsed time to first bite.
Category of protection A-H
(significantly different mean
complete protection time;
ANOVA & Tukey's). No need
to recalculate the results to
"real condition" (simulate real
condition)
Dossier
Hummel,
E.,
Kleeberg,
H. 1997.
in:
Practice
orientate
d results
on use
and
productio
n of
NeemIngredien
ts and
Pheromo
nes V.
Proceedi
ngs of the
5th
workshop
, Wetzlar,
Germany,
January
22-25,
1996
Not
available
Effect of the neem

extract formulation
neemazal-t/s on the
green pea aphid
acyrthosiphon pisum in
the laboratory (1995),
in: Practice orientated
results on use and
production of NeemIngredients and
Pheromones V
19
Repellency of Two
Formulations against
Ants (in-house
laboratory study)
19
Dossier
Vinyl floor tiles were sprayed

with the repellent solution and
placed in cages. A food
attractant was placed in the
centre of each tile. The
number of ants crossing each
tile was counted at 30 and 60
minutes after tiles were placed
into cages. Data for 30 and 60
min was combined and
averaged. Repellency was
also measured the day after
101
Manufacturer
REFERE
NCE
TITLE
PT
Not
available
Repellency of Two
product formulations
against the German
Cockroach (in-house
laboratory study)
19
OPPTS
810.3700
public
draft
SABS
695
Insect Repellents For

Human Skin and
Outdoor Premises
19
Own search
evaluation of the
efficacy of mosquito
repellents - first
revision
Insect repellents for
human skin and
outdoor premises
19
Manufacturer
19
UK guidelines
Durability and
resistance to fouling Marine underwater
paint systems
Standard Method for
Testing Antifouling
Panels in Shallow
Submergence
21
Simulated field test
TM II05
21
Simulated field raft test,

screening test in shallow
marine environments
A standard antifouling panel of
known performance serves as
a control. Subcommittee
D01.45 has a revised rating
procedure now being
evaluated by round robin.
Simulated field test.
Determination of antifouling
performance and reduction of
thickness of marine antifouling
coatings by erosion or under
specified conditions of
hydrodynamic shear stress in
seawater alternated with static
exposure in seawater.
An antifouling coating system
of known performance is
TNsG PE
US EPA
Guideline
OPPTS
810.3700
(1999)
AS
1580.481
.5
(1994)
ASTM
D362378a(2004
)
ASTM
D4939-89
(2007)

for Subjecting Marine
Antifouling Coating to
Biofouling and Fluid
Shear Forces in
Natural Seawater
21

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
the fourth consecutive
application. Sufficient
repellency was defined as
>65%.
The cockroaches had a choice
of two shelters, one treated
with the product and one
untreated. Repellency after 1,
3, 7 and 14 days was
recorded. Percent repellency
was calculated. Sufficient
>65%, excellent repellency
was defined as >85%.
102
TYPE OF
REFERENCE
SOURCE
Manufacturer
TM II 05
UK guidelines
TM II05
REFERE
NCE
TITLE
PT
ASTM
D547994(2007)

Testing Biofouling
Resistance of Marine
Coatings Partially
Immersed
21
ASTM
D561894(2005)

for Measurement of
Barnacle Adhesion
Strength in Shear
21
ASTM
D6990-05

Evaluating Biofouling
Resistance and
Physical Performance
of Marine Coating
Systems
Antifouling coatings methods for the
generation of efficacy
data
21
CEPE
Antifoulin
g
Working
Group
1993
EN ISO
10253:20
06
21

(IF TEST METHOD
AVAILABLE OR
FROM ELSEWHERE)
included to serve as a control
in antifouling studies.
Testing in-situ partial
immersion exposure.
A negative control (non toxic
surface) is applied and should
show heavy fouling accretion
for the test to be valid.
Screening test, measuring
barnacle adhesion in shear to
surfaces exposed in the
marine environment. Surfaces
with known barnacle adhesion
strengths are included to serve
as controls.
Guidance to a panel inspector
for quantitative and consistent
evaluation of coating
performance from test panels
coated with marine antifouling
coating systems.
Simulated field raft test
TYPE OF
REFERENCE
SOURCE
TM II05
TM II05
TM II05
TNsG PE
TM II05
UK guidelines
Water quality - Marine

algal growth inhibition
test with Skeletonema
costatum and
Phaeodactylum
tricornutum (ISO
10253:2006)
21
Growth inhibition test
103
TM II05
Table 2. Overview of efficacy test references, in order of reference.

REFERE
NCE
TITLE
PT
AATCC
100 1999
Antibacterial Finishes
on Textile Materials:
Assessment of
Antibacterial Activity
Assessment of Textile
Materials: Parallel
Streak Method
Assesment of the AntiHouse Dust Mite
Properties
of Textiles under LongTerm Test Conditions
Antifungal Activity,
Assessment on Textile
Materials:
Mildew and Rot
Resistance of Textile
Materials
Comparative Evaluation
of Alternative Halogenbased Disinfection
Strategies
Standard Methods for

the Examination of
(SMEWW).
AATCC
147 1998
AATCC
194-2006
AATCC
30 - 1999
Alleman,
J.E.,
Etzel,
J.E.,
Gendron,
D., Kirsch,
E.J.,
Conley,
J., Fidelle,
T., Handy,
F., and
Hildebran
nd
dt, M. 42
Purdue
Industrial
Waste
Conferenc
e, May
1987,
519-524
American
Public
Health
Associatio
n, 16th
Edition,
1985
TYPE OF
REFERENCE
SOURCE
SHORT TEST
DESCRIPTION (IF TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
Applied to textiles
Applied to textiles
Manufacturer
18
Applied to textiles
Manufacturer
Applied to textiles
Manufacturer
1-5
Microorganisms were
exposed to a mixture of
biocide in solution for 4 or
16 minutes. The
percentage survival was
assessed under various
conditions such as high or
low nitrogen (as
ammonium), high pH or low
temperatures.
Manufacturer
1-5
A swimming pool trial was

performed over a three
month period to
demonstrate the
disinfection efficacy of the
product. Water samples
were collected for microbial
analysis. Water and, air
temperature, number of
bathers (at the time when
samples were taken and
Manufacturer
104
Manufacturer
REFERE
NCE
TITLE
PT
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
total bathers for the day),
eye and skin irritation and
water clarity were also
measured.
Criteria for effective control
was determined as follows:
The state of California,
Department of Health
Services requirement for
pool water is;
Bacteriological quality of
water in the swimming pool
shall be such that not more
than two consecutive
samples, taken when the
pool is in use shall:
1. Contain more than 200
bacteria per ml, as
determined by the standard
plate count; or
2. Contain a total coliform
organism count of 2.2 or
greater per 100 ml of
sample
3. Chemical quality of water
in the pool shall not cause
irritation of eyes or skin of
the bathers, or have other
objectionable physiological
effects on bathers.
TYPE OF
REFERENCE
SOURCE
AOAC
(Associati
on of
Official
Analytical
Chemists)
official
method
965.13
AS
1580.481.
5
(1994)
ASTM
G2196(2002)
Disinfectant (water) for

Swimming Pools
official method of
Analysis, 16th Edition,
1995
1-5
Durability and
resistance to fouling Marine underwater paint
systems
determining resistance
of synthetic polymeric
materials to fungi
21
Simulated field test
TM II05
This practice covers

determination of the effect
of fungi on the properties of
synthetic polymeric
materials in the form of
molded and fabricated
articles, tubes, rods,
sheets, and film materials.
Own searches
Manufacturer
105
REFERE
NCE
TITLE
PT
ASTM
E723-07

for Efficacy of
Antimicrobials as
Preservatives for
Aqueous-Based
Paper Industry
11 or
12?
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
Conditions favorable for
attack, namely, a
temperature of 2 to 38C
(35 to 100F) and a relative
humidity of 60 to 100 %
need to be established.
Expected effects include
surface attack,
discoloration, loss of
transmission (optical), and
removal of susceptible
plasticizers, modifiers, and
lubricants, resulting in
increased modulus
(stiffness), changes in
weight, dimensions, and
other physical properties,
and deterioration of
electrical properties such
as insulation resistance,
dielectric constant, power
factor, and dielectric
strength.
Since attack by organisms
involves a large element of
chance due to local
accelerations and
inhibitions, the order of
reproducibility may be
rather low. To ensure that
estimates of behavior are
not too optimistic, the
greatest observed degree
of deterioration should be
reported.
Conditioning of the
specimens, such as
weathering, heat treatment,
etc., may have significant
effects on the resistance to
fungi. Determination of
these effects is not covered
in this practice.
Laboratory test method is
used to determine the
efficacy of an antimicrobial
for preventing bacterial
spoilage of in-process
solutions, pulp slurries,
106
TYPE OF
REFERENCE
SOURCE
Communication
with UK (HSE)
REFERE
NCE
TITLE
PT
(Bacterial Spoilage)
ASTM
E87500(2005)

for Efficacy of Fungal
Control Agents as
Preservatives for
Aqueous-Based
Paper Industry
11 or
12?
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
starch solutions, polymers,
sizing agents, latex
emulsions, and other
aqueous-based materials
used in the paper industry
from bacterial spoilage.
Test organisms may vary
but should lie above 10E6
CFU/ml.
Bacterial numbers in the
sample are determined at
variable time periods and
compared to a control with
no bactericide. Material to
be preserved is used as
substrate, but for some
materials nutrients may be
added. Results are
expressed as % kill:
[(control plate count-test
plate count)/ control plate
count]x100 at each
sampling time.
Laboratory method that
determines if a fungal
control agent is effective to
preserve pigment
suspensions, dye solutions,
pulp slurries, starch
agents, latex emulsions,
and other specific aqueousbased materials to prevent
spoilage of in-process
aqueous-based products
used in the paper industry.
Will also prevent spore
germination. Plates are
incubated for 7d or until
control plates shos
sufficient growth for rating.
The exposure time should
correspond to the real time
desired, often 6w.
Separate evaluations
should be made on a
representative type for
each specific class of
product to be preserved.
Growth is determined by
visible signs of
107
TYPE OF
REFERENCE
SOURCE
Communication
with UK (HSE)
REFERE
NCE
TITLE
PT
ASTM
E645-07

for Efficacy of
Microbicides Used in
Cooling Water Systems
11
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
deterioration in the sample
and by obtaining fungal
numbers and comparing
them to a sample without
fungal control agents. A
proper level of fungal
control agent prevents
deterioration and reduces
and keeps organisms to an
acceptable level in the test
material, which is
determined by the tester or
user. Often a scale from 0
to 4 (heavy growth).
Efficacy of microbicides
(algicides, bactericides,
and fungicides), evaluated
using simulated or real
cooling tower water against
(1) microbes from cooling
water, (2) microbes in
microbiological deposits
(biofilms) from operating
cooling systems, or (3)
microorganisms known to
contaminate cooling water
systems, or a combination
thereof. Choice of
enumerating method may
vary, e g pour plate, spread
plate, MPN techniques etc.
Test concentrations also
vary but are usually
between 1 and 50 mg/l.
Exposure time varies with
mode of action but includes
3h contact time. For
bacteria 48 h incubation,
fungi 48h-7d, for algae 514d.
Even if using cooling water
and deposits/biofilm
obtained from the field,
laboratory results may not
be totally predictive of
microbicidal effectiveness
in the field (due to e g
variability in environmental
factors). If solvents are
added, a solvent control is
included. If initial count in
108
TYPE OF
REFERENCE
SOURCE
Manufacturer
REFERE
NCE
TITLE
PT
ASTM
D2574-06

for Resistance of
Emulsion Paints in the
Container to Attack by
Microorganisms
for Resistance to Mold
Growth on Interior
Coated Building
Products in an
Environmental Chamber
ASTM
WK8681
10
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
cooling water control
samples are <10E5 bact/ml
(or <10E3 CFU/ml fungi or
algae), the test is invalid. In
addition, the viable counts
of fungi/algae in the control
must at least be equal to
the numbers at time 0. The
untreated control should
show a stable population
with no more than 0.5 log
increase or decrease in
growth during the test
perod. Results are
expressed as Log
reduction in number of
microorganisms at each
biocide concentration: log
(initial count of
microorganisms of the
control sample) log
(number of microorganisms
detected at a given biocide
concentration after a
specific contact time). If 1
log reduction: corresponds
to 90% kill, if 2 log
reduction corresponds to
99% kill, 3 log reduction to
99.9% kill.
TYPE OF
REFERENCE
SOURCE
Own searches
This test method can be

used to evaluate the
coated building products to
accelerated mold growth.
Performance at a certain
rating does not imply any
specific period of time for a
mold free surface.
However, a better rated
panel would be expected to
perform better in actual end
use.
An environmental chamber
and the conditions of
operation to evaluate in a
109
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REFERE
NCE
TITLE
PT
ASTM
WK 17314
Test Method for

Evaluating the Efficacy
of a Liquid Microbiocide
against Biofilm Bacteria
12
ASTM
WK14960
Test Method for

for the Resistance of
Mold Growth on
Uncoated Interior
Building Products in an
Environmental
Chamber.
10
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
4-week period the relative
resistance of coated
building products to surface
mold growth in a controlled
interior environment is
described. The apparatus
is designed so it can be
easily built or obtained by
any interested party.
A procedure for testing the
efficacy of a liquid
microbicide against biofilm
grown on coupons
removed from biofilm
reactors standardized to
grow a repeatable biofilm,
such as the CDC Biofilm
Reactor (ASTM Method E
2562) or Rotating Disk
Biofilm Reactor (ASTM
Method E 2196). The
preparation of the liquid
microbicide and exposure
time are completed
according to
manufacturers instructions
for use. The liquid
microbicide is tested at
room temperature under
static conditions. Biofilm
population density is
recorded as log10 colony
forming units per surface
area. Efficacy is
determined by calculating
the log reduction in viable
cells.
The microbicide is tested at
its use dilution for the
recommended time.
This test method can be
uncoated building products
to accelerated mold growth.
The apparatus
(environmental chamber) is
interested party.
4-week evaluation period.
110
TYPE OF
REFERENCE
SOURCE
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REFERE
NCE
TITLE
PT
ASTM
E151799(2006)

for Determining the
Effectiveness of Liquid,
Gel, Cream, or
Shampoo Insecticides
Against Human Louse
OVA
18
ASTM
D1006-93
Standard
Recommended Practice
for Conducting Exterior
Exposure Tests of
Paints on Wood
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
Relative resistance of
uncoated interior building
products to surface mold
growth in a controlled
interior environment is
evaluated, but performance
at a certain rating does not
imply any specific period of
time for a mold free
surface.
Determines the
effectiveness of ovicidal
materials in liquid, gel,
cream, or shampoo form
against the ova (that is,
eggs or nits) of the human
louse, Pediculus humanus.
This test method consists
of five replicates for a
statistical comparison of
formulations.
Field test: aids in
evaluating the performance
of house and trim paints to
new, previously unpainted
wood exposed to the
environment.
Exposures in several
locations with different
climates which represent a
broad range of anticipated
service conditions are
recommended and several
years of repeat exposures
are needed to get an
average test result for a
given location. Solar
radiation varies
considerably as function of
time of year, and can cause
large differences in the
apparent rate of
degradation in many
polymers. Comparing
results for materials
exposed for short periods
(less than onee year) is not
recommended unless
materials are exposed at
the same time in the same
location.
111
TYPE OF
REFERENCE
SOURCE
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REFERE
NCE
TITLE
PT
ASTM
D327300(2005)

for Resistance to
Growth of Mold on the
Surface of Interior
Coatings in an
Environmental Chamber
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
This test method describes
a small environmental
chamber and the conditions
of operation to evaluate
reproducibly in a 4-week
period the relative
resistance of paint films to
surface mold fungi, mildew
growth in a severe interior
environment. The
apparatus is designed so it
can be easily built or
obtained by any interested
party and will duplicate
results obtained in a large
tropical chamber.
An accelerated test for
determining the resistance
of interior coatings to mold
growth; useful in
estimating the performance
of coatings designed for
use in interior environments
that promote mold growth
and in evaluating
compounds that may inhibit
such growth and the
aggregate levels for their
use.
Used to evaluate the
interior coating to
accelerated mildew growth.
Performance at a certain
rating (in accordance with
Test Method D3274) does
not imply any specific
period of time for a fungal
free coating. However, a
better rated coating nearly
always performs better in
actual end use. This test
method is intended for the
accelerated evaluation of
an interior coatings'
defacement. Use of this
test method for evaluating
exterior coatings'
performance has not been
validated, nor have the
112
TYPE OF
REFERENCE
SOURCE
Own searches
REFERE
NCE
TITLE
PT
ASTM
D345686(2002)

Determining by Exterior
Exposure Tests the
Susceptibility of Paint
Films to Microbiological
Attack
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
limitations for such use
been determined. Any
accelerated weathering
(leaching, weathering
machine exposure, etc.,)
should be reported and
should also bear reference
to the fact that it is beyond
the current scope of this
test method.
Temperature and humidity
must be effectively
controlled within the
relatively narrow limits
specified in order for the
chamber to function
reproducibly during the
short test period. Severity
and rate of mold growth on
a film is a function of the
moisture content of both
the film and the substrate.
A relative humidity of 95 to
98 % at a temperature of
32.5+ 1oC (90+ 2oF ) is
necessary for test panels to
develop rapidly and
maintain an adequate
moisture level to support
mold growth.
This practice provides
guidelines for determining
the susceptibility of paint
films to microbiological
attack on exterior
exposure. The degree to
which microbiological
discoloration occurs is the
primary concern. This
practice covers the
preparation of coatings for
testing, their application on
substrates, and the
arrangement of the coated
panels on exterior test
fences to determine the
degree of microbiological
attack that may occur on
the surface of the coatings
over a period of time.
This practice is intended to
113
TYPE OF
REFERENCE
SOURCE
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REFERE
NCE
TITLE
PT
ASTM
D362378a(2004)
Standard Method for

Testing Antifouling
Panels in Shallow
Submergence
21
ASTM
D4131-84
(2005)
ASTM
D4300-01
ASTM
D457601(2006)

sampling fish with
Rotenone
Standard Test Methods
for Ability of Adhesive
Films to Support or
Resist the Growth of
Fungi

for Mold Growth
Resistance of Wet Blue
17
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
provide guidelines for, and
a discussion of, the various
factors critical in selection
of exterior coatings
resistant to discoloration or
disfigurement by algae and
fungi.
Simulated field raft test,
screening test in shallow
marine environments
A standard antifouling
panel of known
performance serves as a
control. Subcommittee
D01.45 has a revised rating
procedure now being
evaluated by round robin.
Adaptable to both lotic and
lentic situations in littoral
and limnetic areas.
Tests the ability of
adhesive films to inhibit or
support the growth of
selected fungal species
growing on agar plates by
providing means of testing
the films on two agar
substrates, one which
promotes microbial growth,
and one which does not.
constituents.
These test methods are not
appropriate for all
adhesives. The activity of
certain biocides may not be
demonstrated by these test
methods as a result of
irreversible reaction with
some of the medium. As an
example, quaternary
ammonium compounds are
inactivated by agar. A test
method is included for use
with low-viscosity
adhesives along with an
alternative method for use
with mastic-type adhesives.
Determination of mold
growth resistance of wet
blue subject to storage and
114
TYPE OF
REFERENCE
SOURCE
TNsG PE
TM II 05
UK guidelines
Communication
with UK (HSE)
Own searches
REFERE
NCE
ASTM
D478301e1
TITLE

for Resistance of
Adhesive Preparations
in Container to Attack
by Bacteria, Yeast, and
Fungi
PT
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
shipping requirements and
intended for use in leather
manufacturing, assisting
the prediction of storage
time before molding occurs.
This test method may not
be suitable to evaluate
fungicides that are
inactivated by proteins,
including
alkyldimethylbenzyl
ammonium chlorides.
To allow use of this test
method by any laboratory,
flexibility has been
permitted in times,
temperature, and humidity
of incubation, inoculum,
hide sampling area, and
choice of control. These
may be adjusted to fit local
conditions but must be
standardized.
Conclusions about mold
growth resistance are
drawn from the results by
comparing the test with a
simultaneously run control
of known resistance.
Success or failure is
determined by the amount
of mold growth relative to
the control.
The degree of correlation
between this test and
commercial quantities of
wet blue in storage or
shipment situations, or
both, has not been fully
determined.
resistance of liquid
adhesive preparations to
microbial attack in the
container by challenging
adhesive specimens with
cultures of bacteria, yeast,
or fungi, and checking for
their ability to return to
sterility.
115
TYPE OF
REFERENCE
SOURCE
TNsG on Prod
Eval
REFERE
NCE
TITLE
PT
ASTM
D4939-89
(2007)

for Subjecting Marine
Antifouling Coating to
Biofouling and Fluid
Shear Forces in Natural
Seawater
21
ASTM
D547994(2007)

Testing Biofouling
Resistance of Marine
Coatings Partially
Immersed
21
ASTM
D558997(2002)

for Determining the
Resistance of Paint
Films and Related
Coatings to Algal
Defacement
ASTM
D559000(2005)

for Determining the
Resistance of Paint
Films and Related
Coatings to Fungal
Defacement by
Accelerated Four-Week
Agar Plate Assay
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
These test methods return
qualitative results
Simulated field test.
Determination of antifouling
performance and reduction
of thickness of marine
antifouling coatings by
erosion or under specified
conditions of hydrodynamic
shear stress in seawater
alternated with static
exposure in seawater.
An antifouling coating
system of known
performance is included to
serve as a control in
antifouling studies.
Testing in-situ partial
immersion exposure.
A negative control (non
toxic surface) is applied
and should show heavy
fouling accretion for the test
to be valid.
resistance of a paint or
coating film to algal growth.
This test method should not
be used as a replacement
for exterior exposure since
many other factors, only a
few of which are listed will
affect those results.
resistance of two or more
paints or coating films to
fungal growth.
This test method should not
be used as a replacement
for exterior exposure (that
is, Practice D 3456) since
many other factors, only a
few of which are listed will
affect those results.
Comparative evaluation of
different coating
formulations for their
116
TYPE OF
REFERENCE
SOURCE
TM II05
TM II05
Own searches
Own searches
REFERE
NCE
TITLE
PT
ASTM
D561894(2005)

for Measurement of
Barnacle Adhesion
Strength in Shear
21
ASTM
D6990-05

Evaluating Biofouling
Resistance and
Physical Performance
of Marine Coating
Systems
21
ASTM
E105296(2002)

for Efficacy of
Against Viruses in
Suspension
1-5
ASTM
E105397(2002)

for Efficacy of Virucidal
Agents Intended for
1-5
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
relative performance under
a given set of conditions. It
does not imply that a
coating that resists growth
under these conditions will
necessarily resist growth in
the actual application.
Round-robin testing of this
test method versus exterior
exposure is planned.
Screening test, measuring
barnacle adhesion in shear
to surfaces exposed in the
marine environment.
Surfaces with known
barnacle adhesion
strengths are included to
serve as controls.
Guidance to a panel
inspector for quantitative
and consistent evaluation
of coating performance
from test panels coated
with marine antifouling
coating systems.
Laboratory suspension test
that determines the
effectiveness of
antimicrobial solutions
against designated
determined using cell
cultures as the host system
for specific viruses. For
special applications of
virucides, such as
inactivation of viruses in
contaminated liquid wastes,
and as a first stage in
determining virucidal
potential of liquid chemical
germicides, liquid hand
soaps, OTC topicals or
other skin products.
Laboratory test method.
Evaluates the virucidal
efficacy of liquid, aerosol,
117
TYPE OF
REFERENCE
SOURCE
TM II05
TM II05
Manufacturer
Manufacturer
REFERE
NCE
TITLE
PT
Inanimate
Environmental Surfaces
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
or trigger spray
antimicrobial solutions on
inanimate nonporous
environmental surfaces,
against designated
determined utilizing cell
cultures as the host system
for specific viruses. Efficacy
is measured by a
percentage reduction in
titer.
Effectiveness of
procedures and agents for
inactivating (neutralizing,
quenching) the
microbiocidal properties of
antimicrobial agents and to
ensure that no components
of the neutralizing
procedures and agents,
themselves, exert an
inhibitory effect on
microorganisms targeted
for recovery.
TYPE OF
REFERENCE
SOURCE
ASTM
E1054-02

for Evaluation of
Inactivators of
1-5
ASTM
E1115-02
Test Method for

Evaluation of Surgical
Hand Scrub
Formulations
for Efficacy of Sanitizers
Recommended for
Inanimate Non-Food
Contact Surfaces
of a Evaluation of a
Preoperative,
Precatheterization, or
Preinjection Skin
Preparations
Test Method for
Evaluation of the
Effectiveness of Health
Care Personnel
Handwash Formulations
1-5
Own searches
1-5
Own searches
1-5
Own searches
1-5
Own searches

Evaluation of
Antimicrobials in Liquid
ASTM
E1153-03
ASTM
E117301e1
ASTM
E1174-06
ASTM
E1259-05
The procedure should be

relative efficacy of
118
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Own searches
REFERE
NCE
TITLE
PT
Fuels Boiling Below

390C
ASTM
E1327-07
ASTM
E142700e1
ASTM
E142899(2004)

for Evaluation of
Antimicrobial Handwash
Formulations by
Utilizing Fingernail
Regions
Standard Guide for
Selecting Test Methods
to Determine the
Effectiveness of
and Other Chemicals
for the Prevention,
Inactivation and
Removal of Biofilm
1-5

for Evaluating the
Performance of
Antimicrobials in or on
Polymeric Solids
Against Staining by
Streptoverticillium
reticulum (A Pink Stain
Organism)
12
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
microbicides in liquid fuels
boiling below 390C. The
effect of environmental
conditions, such as a
variety of fuel additives,
metal surfaces, and
climatology, are variables
that can be included in
specific tests using this
protocol.
TYPE OF
REFERENCE
SOURCE
Own searches
The purpose of this guide is

to inform the investigator of
methods that can be used
for biofilm formation and
measurement, allowing
development of test
procedures for determining
the effectiveness of
chemical treatments for
prevention, inactivation,
and removal of unwanted
biofilm. This guide is not an
exhaustive survey of biofilm
methods.
This test method provides a
technique for evaluating
antimicrobials in or on
polymeric solids against
staining by
Streptoverticillium
reticulum, and should
assist in the prediction of
performance of treated
articles under actual field
conditions. Conditioning of
the specimens, such as
weathering, and heat
treatment, may have
significant effects on
performance of
antimicrobials against
staining. Determination of
these effects is not
included in this test
119
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REFERE
NCE
TITLE
PT
ASTM
E1482-04

for Neutralization of
Virucidal Agents in
Virucidal Efficacy
Evaluations
for Evaluation of First
Aid Antiseptic Drug
Products
Effectiveness of
Sterilization Processes
Devices
to Determine Efficacy of
Disinfection Processes
Devices (Simulated Use
Test)
for Determining the
Virus-Eliminating
Effectiveness of Liquid
Hygienic Handwash and
Handrub Agents Using
the Fingerpads of Adult
Volunteers
Standard test method
for efficacy of slimicides
for the paper industry
slime
1-5
Own searches
1-5
Own searches
1-5
Own searches
1-5
Own searches
1-5
Own searches
ASTM
E1589-05
ASTM
E176695(2002)
ASTM
E183796(2002)
ASTM
E1838-02
ASTM
E1839-07
12
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
method. This test method is
also not suitable for
evaluating dark-pigmented
test samples.
A procedure to evaluate the

efficacy of slimicides for the
control of bacterial and
fungal slimes in paper mill
systems and their
counterparts. This test
method is run in acid,
alkaline, or acid and
alkaline conditions to
determine the efficacy of
the slime control agent.
The test conditions may be
modified to reflect intended
use patterns in typical
paper mill systems,
including use of actual
paper mill furnish. Efficacy
based on a % reduction on
120
TYPE OF
REFERENCE
SOURCE
Communication
with UK (HSE)
Manufacturer
REFERE
NCE
TITLE
PT
ASTM
E1882-05

for Evaluation of
Antimicrobial
Formulations by the
Agar Patch Technique
1-5
ASTM
E1883-02

for Assessment of an
Antibacterial Handwash
Product by Multiple
Basin Wash Technique
Standard Guide for
1-5
Own searches
Own searches
ASTM
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
CFU.
This test method
determines the antibacterial
activity and persistence of
test formulations, as
measured by the inhibition
of a test organism on an
agar surface exposed to
test sites on human skin
treated with the
formulations. This
procedure can be used to
evaluate formulations
containing ingredients
intended to inhibit growth of
bacteria on intact skin and
measures the difference,
post-product-exposure,
between numbers of
bacterial colonies on active
test formulation plates and
numbers on control plates,
expressed as percent
inhibition.
This procedure may also
be used to test for
persistence of activity, as a
function of time elapsed
between application of
active test formulation and
application of active test
plates. Because no
procedure for neutralization
of the antimicrobial action
of active ingredients can be
included in the test, the
agar patch method is
limited to the extent that
results expressed as
percent inhibition do not
differentiate between
bacteriostatic and
bacteriocidal effects and,
hence, must not be
portrayed as reductions.
121
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REFERENCE
SOURCE
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REFERE
NCE
TITLE
E189197(2002)
Determination of a
Survival Curve for
Against Selected
Microorganisms and
Calculation of a D-Value
and Concentration
Coefficient
for Evaluation of
Handwashing
Formulations for VirusEliminating Activity
Using the Entire Hand
Carrier Test Method to
Evaluate the
Bactericidal, Fungicidal,
Mycobactericidal, and
Sporicidal Potencies of
Liquid Chemical
Microbicides
ASTM
E2011-99
ASTM
E2111-05
PT
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
1-5
1-5
TYPE OF
REFERENCE
SOURCE
Own searches
This test can be performed

with or without a soil load to
determine the effect of
such loading on
microbicide performance.
The soil load developed for
this test is a mixture of
three types of proteins
(high molecular weight
proteins, low molecular
weight peptides, and
mucous material) to
represent the body
secretions, excretions, or
other extraneous
substances that chemical
microbicides may
encounter under field
condititions.
This test method is
designed for use in product
development and for the
generation of product
potency data. This test
method is fully quantitative
and it also avoids any loss
of viable organisms through
wash off. It permits the
loading of each carrier with
a known volume of the test
organism. The
incorporation of controls
can also determine the
initial load of colony
forming units (CFU) of
organisms on the test
carriers and any loss in
122
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REFERE
NCE
TITLE
PT
ASTM
E2149-01
Determining the
Antimicrobial Activity of
Immobilized
Under Dynamic Contact
Conditions
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
CFU after the mandatory
drying of the inoculum. This
test method is designed to
have survivors and also to
be used with a
performance standard. The
surviving microorganisms
on each test carrier are
compared to the mean of
no less than three control
carriers to determine if the
been met. To allow proper
statistical evaluation of
results, the size of the test
inoculum should be
sufficiently large to take
into account both the
performance standard and
the experimental variation
in the results.
Evaluates the resistance of
non-leaching antimicrobial
treated specimens to the
growth of microbes under
dynamic contact conditions.
This test determines the
antimicrobial activity of
treated specimen by
shaking samples of surface
bound materials in a
concentrated bacterial
suspension for a one hour
contact time or other
contact times as specified
by the investigator. The
suspension is serially
diluted both before and
after contact and cultured.
This dynamic shake flask
test was developed for
routine quality control and
screening tests in order to
overcome difficulties
(including ensuring contact
of inoculum to treated
surface) in using classical
antimicrobial test methods
to evaluate substratebound antimicrobials. This
test also allows for the
123
TYPE OF
REFERENCE
SOURCE
Manufacturer,
applied to textiles
REFERE
NCE
TITLE
PT
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
versatility of testing
contamination due to such
things as hard water,
proteins, blood, serum,
various chemicals, and
other contaminates or
physical/chemical stresses
or manipulations of the
specimens of interest.
The number of viable
organisms in the
suspension is determined
and the percent reduction
is calculated based on
initial counts or on
retrievals from appropriate
untreated controls. his
method is intended for
those surfaces having a
percent reduction activity of
50 % to 100 % for the
specified contact time.
Surface antimicrobial
activity is determined by
comparing results from the
test sample to
simultaneously run
controls. The presence of a
leaching antimicrobial is
both pre- and postdetermined by the
presence of a zone of
inhibition.
TYPE OF
REFERENCE
SOURCE
ASTM
E2180-07

for Determining the
In Polymeric or
This test method is

designed to evaluate
(quantitatively) the
antimicrobial effectiveness
of agents incorporated or
bound into or onto mainly
flat (two dimensional)
hydrophobic or polymeric
surfaces. The method
focuses primarily on
assessing antibacterial
activity; however, other
microorganisms such as
yeast and fungal conidia
may be tested using this
method. The vehicle for the
inoculum is an agar slurry
Own searches
124
REFERE
NCE
TITLE
PT
ASTM
E2196-07

for Quantification of a
Pseudomonas
aeruginosa Biofilm
Grown with Shear and
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
which reduces the surface
tension of the saline
formation of a "pseudobiofilm," providing more
even contact of the
inoculum with the test
surface. This test method
facilitates the testing of
hydrophobic surfaces by
utilizing cells held in an
agar slurry matrix.
This method can confirm
the presence of
antimicrobial activity in
plastics or hydrophobic
surfaces and allows
determination of
antimicrobial activity
between untreated plastics
or polymers and those with
bound or incorporated low
agents. Comparisons
between the numbers of
survivors on preservativetreated and control
hydrophobic surfaces may
also be made. This test
method, as written, is
inappropriate to determine
efficacy against biofilm
cells, which are different
both genetically and
metabolically than
planktonic cells used in this
test. The procedure also
permits determination of
"shelf-life" or long term
durability of an
antimicrobial treatment
which may be achieved
through testing both nonwashed and washed
This test method is used for
growing a repeatable
biofilm in a continuously
stirred flow reactor. In
125
TYPE OF
REFERENCE
SOURCE
Own searches
REFERE
NCE
TITLE
PT
Continuous Flow Using

a Rotating Disk Reactor
ASTM
E2197-02
Disk Carrier Test
Method for Determining
the Bactericidal,
Virucidal, Fungicidal,
Mycobactericidal and
Sporicidal Activities of
Liquid Chemical
Germicides
1-5
ASTM
E2274-03

for Evaluation of
Disinfectants
Evaluating WaterMiscible Metalworking
Fluid Bioresistance and
Antimicrobial Pesticide
Performance
1-5
ASTM
E227503e1
(replaces
D3946
and E686)
13
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
addition, the test method
describes how to sample
and analyze biofilm for
viable cells. In this test
method, biofilm population
surface area.
The method is designed to
evaluate the ability of liquid
chemical germicides to
inactivate vegetative
bacteria, viruses, fungi,
mycobacteria and bacterial
spores in the presence of a
soil load on disk carriers
that represent
environmental surfaces and
medical devices. It is also
designed to have survivors
that can be compared to
mean of no less than three
control carriers to
determine if the
been met. For proper
statistical evaluation of the
results, the size of the test
inoculum should be
sufficiently large to take
into account both the
performance standard and
the experimental variation
in the results. The test
protocol does not include
any wiping or rubbing
action. It is, therefore, not
designed for testing
germicide-soaked wipes.
TYPE OF
REFERENCE
SOURCE
Own searches
Own searches
Laboratory procedures for

rating the relative inherent
bioresistance of watermiscible metalworking
fluids, the bioresistance
attributable to
augmentation with
antimicrobial pesticides or
126
TNsG on Prod
Eval
REFERE
NCE
TITLE
PT
ASTM
E2276-

for Determining the
1-5
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
both, for determining the
need for microbicide
addition prior to or during
fluid use in metalworking
systems and for evaluating
microbicide performance.
Relative bioresistance is
determined by challenging
metalworking fluids with a
biological inoculum that
may either be
characterized (comprised
of one or more known
biological cultures) or
uncharacterized
(comprised of biologically
contaminated metalworking
fluid or one or more
unidentified isolates from
deteriorated metalworking
fluid). Challenged fluid
bioresistance is defined in
terms of resistance to
biomass increase, viable
cell recovery increase,
chemical property change,
physical property change or
some combination thereof.
This practice is applicable
to antimicrobial agents that
are incorporated into either
the metalworking fluid
concentrate or end-use
dilution. It is also applicable
to metalworking fluids that
are formulated using nonmicrobicidal, inherently
bioresistant components.
The results of tests
completed in accordance
with this practice should be
used only to compare the
relative performance of
products or microbicide
treatments included in a
test series. Results should
not be construed as
predicting actual field
performance.
TYPE OF
REFERENCE
SOURCE
Own searches
127
REFERE
NCE
TITLE
03e1
Bacteria-Eliminating
Effectiveness of
the Fingerpads of Adult
Subjects
Effectiveness of
Cleaning Processes for
Reusable Medical
Instruments Using a
Microbiologic Method
(Simulated Use Test)
ASTM
E2314-03
PT
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
1-5
ASTM
E2315-03
Standard Guide for

Assessment of
Using a Time-Kill
Procedure
1-5
ASTM
E2361-04
Standard Guide for

Testing Leave-On
Products Using In-Situ
Methods
1-5
TYPE OF
REFERENCE
SOURCE
Own searches
This guide covers

examples of a basic
method to measure the
changes of a population of
aerobic microorganisms
within a specified sampling
time when tested against
antimicrobial test materials
in vitro. Several options for
organism selection and
growth, inoculum
preparation, sampling times
and temperatures are
provided. Antimicrobial
activity of specific
materials, as measured by
this technique, may vary
significantly on variables
selected.
This guide covers test
methods and sampling
procedure options for
leave-on products (such as
alcohol hand rubs and
lotions containing
antimicrobial ingredients)
for consumer and hospital
personnel. These products
are distinguished from
conventional washing and
scrubbing preparations in
that they do not rely on the
rinsing, physical removal,
and antimicrobial action in
determining their
effectiveness. Although
128
Own searches
Own searches
REFERE
NCE
TITLE
PT
ASTM
E2362-04

Evaluation of Presaturated or
Impregnated Towelettes
for Hard Surface
Disinfection
for Evaluation of
Disinfectants for Use in
High Efficiency Washing
Operations
for Using Seeded-Agar
for the Screening
Assessment of
Antimicrobial Activity In
Carpets
1-5
Own searches
1-5
Own searches
ASTM
E2406-04
ASTM
E2471-05
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
agitation and friction may
serve to release organisms
from the skin and folds and
crevices, organisms are
then killed in situ and are
not rinsed from the skin
surface before sampling.
Appropriate test methods
for the hands have been
published, while other
sampling methods will be
needed for testing body
areas other than the hands.
Rapid screening evaluating

of antimicrobial (both
antibacterial and antifungal)
activity in or on the carpet
face fiber or incorporated
into the backing structure of
the carpet (or both). The
method simulates actual
use conditions that may
occur on carpets (for
example, food and
beverage spills, soiling
from foot traffic, prolonged
moisture exposure) and
provides a means to screen
for activity and durability of
an antimicrobial treatment
under conditions of organic
loading. This test method
provides for the
simultaneous assessment
of multiple carpet
components for
antimicrobial activity.
Carpets may be cleaned
129
TYPE OF
REFERENCE
SOURCE
Own searches
REFERE
NCE
TITLE
PT
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
prior to testing in order to
assess the durability of the
antimicrobial effect. The
method can assess the
durability of the
antimicrobial treatments on
new carpets, and on those
repeatedly shampooed or
exposed to in-use
conditions.
Half strength (nutrient and
agar) tryptic soy agar is
used as the inoculum
vehicle for bacteria and half
strength potato dextrose
agar as the inoculum
vehicle for mold conidia.
Use of half strength agars
may reduce undue
neutralization of an
antimicrobial due to
excessive organic load.
This test method
simultaneously evaluates
(both visual and stereomicroscopic) antimicrobial
activity both at the fiber
layer and at the primary
backing layer of carpet.
TYPE OF
REFERENCE
SOURCE
ASTM
E2562-07

for Quantification of
Pseudomonas
aeruginosa Biofilm
Grown with High Shear
and Continuous Flow
using CDC Biofilm
Reactor
1-5 +
12
This test method specifies

the operational parameters
required to grow a
repeatable Pseudomonas
aeruginosa biofilm under
high shear (1). The
resulting biofilm is
representative of
generalized situations
where biofilm exists under
high shear rather than
representative of one
particular environment. The
biofilm generated in the
CDC biofilm reactor is also
suitable for efficacy testing.
3 This test method
describes how to sample
and analyze biofilm for
viable cells. Biofilm
population density is
Own searches
130
REFERE
NCE
TITLE
PT
ASTM
E640-06

for Preservatives in
Water-Containing
Cosmetics
1-5
ASTM
E65291(2003)

for Nonresidual Liquid
Household Insecticides
18
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
recorded as log colony
forming units per surface
area. After the 48 h growth
phase is complete, the user
may add the treatment in
situ or harvest the coupons
and treat them individually.
This test method uses the
Centers for Disease
Control and Prevention
(CDC) biofilm reactor. The
CDC biofilm reactor is a
continuously stirred flow
reactor with high wall
shear. Although it was
originally designed to
model a potable water
system for the evaluation of
Legionella pneumophila,
the reactor is versatile and
may also be used for
growing and/or
characterizing biofilm of
varying species.
This test method should be
used to determine if a
preservative or
preservative system has
application for the
preservation of watermiscible cosmetic products.
It sets minimal
requirements for
preservative performance
in model formulations.
efficiency of household and
industrial-use, contact
insecticides dissolved in
base oils and applied in
spray formulations. It is
developed to test
insecticides against house
flies (Musca domestica, L),
but test data may also be
products against
131
TYPE OF
REFERENCE
SOURCE
Own searches
Own searches
REFERE
NCE
TITLE
PT
ASTM
E65391(2003)

Aerosol and
Pressurized Space
Spray Insecticides
18
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
Not designed to measure
the residual action of the
spray formulation.
The test determines the
relative efficacy of aerosol
and pressurized space
spray insecticide
formulations against house
flies (Musca domestica, L)
strains and, with
modifications in dosage,
other flying insects. Test
data obtained by this test
method may also be
product against
This test method is not
residual activity.
The test may be conducted
using approximately 100
house flies per test (small
group) or 500 flies per test
(large group). Selected
reference standards are the
Official Test Aerosol II
(OTA II) for oil based
aerosol products and
Tentative Official Aqueous
Pressurized Spray
(TOAPS) for water based
aerosol products. Aerosol
test knockdowns: % down
of total flies at 5, 10, 15
minutes after application.
Aerosol test knock down
mortality: dead knocked
down x100/total flies.
These numbers should on
average be equal to,
greater than or no more
than 5% points below the
corresponding numbers of
the reference in order to
meet the standard. No
statement on precision or
bias, only whether
132
TYPE OF
REFERENCE
SOURCE
UK guidelines
REFERE
NCE
TITLE
PT
ASTM
E65496(2003)

Aerosol and
Pressurized Spray
Cockroaches
18
Standard guide for

conducting acute
toxicity tests on test
materials with fishes,
17
ASTM
E729-96
(2007)
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
conformance to criteria for
success specified in the
procedure.
crawling insects:
cockroaches Determines
the relative efficiency of
aerosol and pressurised
spray formulations against
cockroaches, but test data
by this test method may
also be adequate to
support claims for use of
the product to control the
exposed or accessible
stages of silverfish, ants,
centipedes, spiders, and
certain stored product
pests. Applied as direct
sprays for 30 s. on last
instar nymphs. Observation
period: 48h. The test is not
residual action.
Ten groups with 20
organisms in each. The test
is run in conjunction with
the Official Test Aerosol II
(OTA II) (or Tentative
Official Aqueous
Pressurized Spray
(TOAPS) as the standard
basis of comparison. The
mortality after 24h should
be between 50 and 75%
when testing with the OTA.
The test specimens meet
the standard if average %
dead and moribound is
equal to, above or within
10% points less than
average % dead of the
OTA series after 48h.
Precision or bias is not
specified, only states
whether conforms to
efficacy criteria.
2 to 8-day exposures,
depending on the species.
Static, renewal, or flowthrough procedures.
133
TYPE OF
REFERENCE
SOURCE
UK guidelines
Communication
with UK (HSE)
REFERE
NCE
ASTM
E938-05
TITLE
macroinvertebrates and
amphibians
Liquid, Gel, or Cream
Adult Human Lice
PT
18
ASTM
E93994(2006)

of Field Testing Topical
Applications of
Compounds as
Repellents for Medically
Important and Pest
Arthropods (Including
Insects, Ticks, and
Mites): Mosquitoes
19
ASTM
E95194(2006)

for Laboratory Testing
of Non-Commercial
Mosquito Repellent
Formulations On the
Skin
19
ASTM
E97991(2004)

for Evaluation of
Antimicrobial Agents as
Preservatives for Invert
Emulsion and Other
Water Containing
Hydraulic Fluids
Evaluating Microbial
Susceptibility of
Nonmetallic Materials
by Laboratory Soil
Burial
13
ASTM
G160-03
810?
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
Usually reported as LC50
or EC50.
crawling insects: human
louse. Only gels or creams
that liquefy at 32C (90F)
can be tested).
Five replicates, for a
statistical comparison of
formulations.
Evaluates the repellency of
promising compounds that
have undergone primary
laboratory studies and
approved for skin
application for secondary
testing.
The method is designed for
the study of mosquito
repellents, but can be
modified to determine the
repellency of candidate
compounds for other flying
insects that attack humans.
Can be used to test the
efficacy of repellent
compounds that can be
diluted with ethanol,
acetone etc. Both biological
effectiveness and
persistence of the repellent
can be assessed.
ED50 and ED95 are
determined for comparative
and practical purposes
respectively. Precision of
the test can be evaluated
(confid intervals).
TYPE OF
REFERENCE
SOURCE
Own searches
Own search
Own search
TNsG on Prod
Eval
A wide variety of properties

may be affected by
microbial attack depending
on material or item
characteristic. Standard
methods (where available)
134
Own searches
REFERE
NCE
TITLE
PT
ASTM
G2996(2002)

Determining Algal
Resistance of Plastic
Films
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
should be used for each
different property to be
evaluated. This practice
does not attempt to
enumerate all of the
possible properties of
interest nor specify the
most appropriate test for
those properties. Test
methods must, however, be
appropriate to the material
being tested. Evaluation of
a nonmetallic material's
microbiological
susceptibility when in
contact with the natural
environment of the soil and
is intended for use on
material test specimens
that are approximately 2
cm (3/4 in.) thick and 100
cm2 (20 in2) or less. It is
recommended that this
practice be combined with
appropriate environmental
exposures (for example,
sunlight simulating
weathering devices, the
hydrolytic effects of
extended aqueous contact,
or extraneous nutrients) or
fabrication into articles (for
example, adhesive bonding
of seams) which may
promote microbiological
susceptibility during the
service life of material.
Microbiological
susceptibility may be
reflected by a number of
changes including staining,
weight loss, or reduction in
tensile or flexural strength.
This practice may be
applied to articles that do
not spend the majority of
their service life in soil.
susceptibility of plastic films
to the attachment and
proliferation of surface-
135
TYPE OF
REFERENCE
SOURCE
Own searches
REFERE
NCE
TITLE
PT
ASTM
WK12880
Standard Guide for

Evaluation of Clean
Room Disinfectants
Revision of E183996(2002) Standard Test
Method for Efficacy of
Slimicides for the Paper
Industry--Bacterial and
Fungal Slime
Test Method for
Determining the TimeKill Kinetics Of
Antimicrobial
Compounds
1-5
Revision of E2180-01
for Determining the
In Polymeric or
ASTM
WK14214
ASTM
WK15324
ASTM
WK16397
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
growing algae, produced in
bodies of water, such as
swimming pools, artificial
ponds, and irrigation
ditches that are lined with
plastic films.
Evaluating the degree and
permanency of protection
against surface growth of
algae afforded by various
additives incorporated in
the film.
TYPE OF
REFERENCE
SOURCE
Own searches
12
Efficacy of slimicides for

the control of bacterial and
fungal slimes in paper mill
systems and their
counterparts.
Own searches
This test method assesses

the microbicidal activity of
antimicrobial materials,
whereby the survival of
organisms exposed to a
water miscible antimicrobial
agent is determined as a
function of time. The
primary purpose of this
method is to provide a set
of standardized conditions
and test organisms to
facilitate comparative
assessments of
antimicrobial materials
miscible in aqueous
systems.
Evaluates (quantitatively)
the antimicrobial
effectiveness of agents
incorporated or bound into
or onto mainly flat (two
dimensional) hydrophobic
or polymeric surfaces. The
method focuses primarily
on assessing antibacterial
activity; however, other
microorganisms such as
yeast and fungal conidia
may be tested using this
Own searches
136
Manufacturer
(updated),
applied to
textiles.
REFERE
NCE
TITLE
PT
ASTM
WK4751
Standard Guide for

Selecting Test Methods
to Determine the
Efficacy of Antimicrobial
Agents and Other
Chemicals for
Sanitization of Produce
1-5
ASTM
WK4757

for the Assessment of
Antimicrobial Activity In
Carpets; Seeded-Agar
Overlay Screen
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
method.This method can
confirm the presence of
antimicrobial activity in
plastics or hydrophobic
surfaces. The vehicle for
the inoculum is an agar
slurry which reduces the
surface tension of the
saline inoculum carrier and
allows formation of a
"pseudo-biofilm," providing
more even contact of the
inoculum with the test
surface.
The method allows
determination of
antimicrobial activity
between untreated plastics
or polymers and those with
bound or incorporated low
agents. Comparisons
between the numbers of
survivors on preservativetreated and control
hydrophobic surfaces may
also be made. The
procedure also permits
determination of "shelf-life"
or long term stability of an
antimicrobial treatment
which may be achieved
through testing both nonwashed and washed
TYPE OF
REFERENCE
SOURCE
Own searches
Designed to evaluate
and effectiveness of
antimicrobial preservatives
in or on carpets. This
method can be used to
evaluate both the
137
Own searches
REFERE
NCE
TITLE
PT
ASTM
WK5097
Guideline For
Evaluating The
Potential For Decline In
Planktonic
Microorganism
Susceptability To
Antimicrobial
Compounds: Healthcare
Applications
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
antibacterial and antifungal
activity
This method is useful for
assessing the durability of
the antimicrobial treatments
because it can be
performed on both new
carpets and those that
have been repeatedly
shampooed or exposed to
in-use conditions.
This method utilizes either
tryptic soy agar as the
inoculum vehicle for
bacteria or potato dextrose
agar as the inoculum
vehicle for mold conidia. .
Full compliment agars can
be used to mimic high soil
or organic loads on carpets
or partial nutrient
complement agars can be
used to mimic lightly soiled
conditions on carpet. This
method allows for the
simultaneous evaluation
(both visual and stereomicroscopic) of
antimicrobial activity both at
carpet.
This method uses an agardilution procedure to
determine the Minimum
Inhibitory Concentration
(MIC) and subsequently to
screen for the development
of decreased susceptibility
for test formulations versus
bacteria or yeast species.
The inocula used on the
second, third, fourth, and
fifth test cycles will be
prepared from the microbial
growth present at the
previous days end
point;i.e., that agar-dilution
plate containing the highest
concentration of the test
formulation (lowest dilution)
138
TYPE OF
REFERENCE
SOURCE
Own searches
REFERE
NCE
TITLE
PT
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
that allows microbial
growth.
TYPE OF
REFERENCE
SOURCE
ASTM
WK6845
[revision
of E 124198 (2004)]
Standard guide for

conducting early lifestage toxicity test with
fishes
17
Communication
with UK (HSE)
ASTM
WK8252
New Standard Test

Method for Determining
Resistance of Aqueous
Metalworking Fluids
towards NonTuberculous,
Environmental
Mycobacteria
13
A flow through test,

beginning before hatch and
ending after hatch. 28 to
120-day (depending on
species) continuous
exposure.
bioresistance of aqueous
metalworking fluids towards
non-tuberculous (NTM),
rapidly growing( RGM),
environmental
mycobacteria by
challenging them with a
mycobacterial inoculum
isolated from actual spoiled
metalworking fluid field
samples from the user/s
site.
In order to simulate field
conditions, another
challenge inoculum
consisting of a mixture of
common metalworking fluid
spoilage microorganisms
originating from actual
MWF field samples is also
used.
ASTM
WK9062
Guideline for Evaluation

of Residual
Effectiveness of
Antibacterial Personal
Cleansing Products
for Determining the
Fungus-Eliminating
Effectiveness of
the Fingerpads of
Adults
Richtlinie fr die
Prfung von
Nagetierbekmpfungsm
itteln gegen Hausmase
1-5
Own searches
1-5
Own searches
14
TNsG on Prod
Eval
ASTM
WK9378
BBA 9 3.1
Own searches
UK guidelines
Manufacturer
139
REFERE
NCE
TITLE
PT
BBA 9 3.2
Richtlinie fr die
Prfung von
Nagetierbekmpfungsm
itteln gegen
Wanderratten
14
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
TNsG on Prod
Eval
UK guidelines
Manufacturer
Bechert et
al., Nature
Medicine
6, 10531056
(2000)
A new method for

screening anti-infective
biomaterials
1-5
The test is based on the

release of vital daughter
cells from the sample
surface into the
surrounding. The
proliferation activity of
these daughter cells, which
are responsible for infection
development, can be
monitored in a time course.
Antimicrobial activity is
monitored by the time
needed to reach a defined
optical density, which is
dependent on the number
of released daughter cells.
All measurements are
performed in comparison to
an untreated control
(without antimicrobial
additive). The difference
between the reference (A)
and the test sample (B) to
reach the threshold OD
gives the degree of
antimicrobial activity. If all
bacteria on the surface of
the material are prevented
from multiplying, no
daughter cells are
produced and the test
object is considered
bactericidal. Materials can
also be antimicrobial, which
means that not all cells on
the test surface are
prevented from growing.
Some cells are able to
divide and release
daughter cells into the
surroundings, which are
then optically registered in
a so-called growth curve. If
surviving daughter cells are
140
Test institute
REFERE
NCE
TITLE
PT
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
grown under controlled
conditions over 48 h
(observation time), a higher
turbidity and therefore a
bigger signal is generated.
At the same time only vital
and proliferative cells are
accounted for.
TYPE OF
REFERENCE
SOURCE
In particular antimicrobial
samples will release
daughter cells into the
surrounding. Hence,
microbial growth is first
observed noticeably later.
This right-shift towards
longer times is indicative
for the antimicrobial
efficacy of the tested
samples. The so-called
onset OD serves as a
quantifiable parameter and
is equivalent to the required
number of hours required
for the surviving daughter
cells to grow to a
predefined optical density
(OD = 0.2).
A material is defined to be
antimicrobial only if the
formation of at least 99.9%
(which is equivalent to a
net onset-OD of 6 hours
and is similar to a killing
rate of 3 log10 steps) of the
daughter cells during the
challenge time is prevented
in comparison with the
blank sample.
BS
6734:200
4
BS 3900
General Purpose
Disinfection Test
1-5
Methods of test for

paints. Part G6.
Assessment of
10
Marketed worldwide by
Ciba Specialty Chemicals
under the tradename
NumetrikaTM.
Mycobacterium fortuitum
Provides only a
methodology for production
of a test surface for
141
Manufacturer
TNsG Prod Eval

UK guidelines
REFERE
NCE
TITLE
PT
growth. BSI London, UK
BS 41721:1999
aerosol dispensers
against houseflies.
Specification for
insecticidal performance
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
exposure by inoculation
with mold growth. The test
therefore has to be
modified to be used as a
test method for assessing
interior surface biocides.
18
TYPE OF
REFERENCE
SOURCE
TNsG on Prod
Eval
TM II05 (Fr)
UK guidelines
BS 41722:1999
BS
6085:199
2
CEB
(1981)
CEB
(1981)
CEB 107
(1985)
CEB 135
(1987)
CEB
135bis
aerosol dispensers
against houseflies.
Method for
determination of
insecticidal performance
18
resistance of textiles
to microbiological
deterioration
to evaluate the efficacy
of rodenticidal products
in rats
Trial method to evaluate

the efficacy of
rodenticidal products
against rats under
the efficacity of
insecticidal products for
the control of stable flies
in premises for the
rearing of domestic
animals under practical
conditions
the efficacy of
insecticidal or miticidal
products for treatments
of storage premises of
products for animals or
plants
to evaluate the efficacy
14
TNsG on Prod
Eval
TM II05 (Fr)
UK guidelines
14
Manufacturer
TMII Fr
TMII Fr
18
18
Surface treatments of
storage premises of
products
TM II05 (Fr)
18
Space treatments
TM II05 (Fr)
142
REFERE
NCE
TITLE
(1996)
of insecticidal products
in premises for the
storage, industrial
processing and sale of
products from animals
or plants
the efficacy of
insecticidal products for
the control of
cockroaches in
buildings under practical
conditions
the efficacy of
insecticidal bait
products against
common species
the efficacy of a
fumigant for insect
control in premises for
the storage, processing
and production of food
the efficacy of fumigants
for insect control in
stored products
Washer-disinfectors Part 5: Test soils and
methods for
demonstrating cleaning
efficacy
CEB 159
(1992)
CEB 196
(1997)
CEB 213
(1999)
CEB 224
(2001)
CEN
158835:2005
CEN/TR
15046:20
05
CEN/TS
12037:20
03
Wood preservatives Artificial weathering of

biological testing - UVradiation and waterspraying procedure
protective effectiveness
PT
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
18
TM II05 (Fr)
18
TM II05 (Fr)
18
TM II05 (Fr)
18
TM II05 (Fr)
1-5
Also ISO method.

Acceptance criteria are
included, based on visual
inspection and/or a
microbiological end-point
as stated for each method.
Where chemical detection
of residual soiling is
required/sought, methods
can be complemented by
the specific determination
of a residual component of
the applied test soil.
Pretreatment procedure
before efficacy testing
Own searches
Own searches
TM II05 (Fr)
143
REFERE
NCE
CEPE
Antifouling
Working
Group
1993
CTBABIO-E 001
CTBABIO-E 002
CTBABIO-E 003
CTBABIO-E 004
CTBABIO-E 005
CTBABIO-E 006
CTBABIO-E 007
CTBABIO-E 008
CTBABIO-E 009
CTBABIO-E 010
Dautel H,
Hilker M,
Kahl O,
Siems K,
2001.
EEC:
1988
EN
1040:200
5
TITLE
of a wood preservative
contact - Horizontal lapjoint method
Antifouling coatings methods for the
generation of efficacy
data
PT
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
21
Simulated field raft test
TNsG PE
TM II05
UK guidelines

treated soils
treated masonry
Field test on bait system
for termite control
Laboratory tests for bait
system for termite
control
Laboratory tests for bait
system for termite
control
Action of U.V. light on
physico-chemical
barrier for termite
Action of alcalin
conditions on physicochemical barrier for
termite control
physico-chemical
barrier for termite
control
Forced test on termite
bait control
Choice test on termite
bait control
Verwendung von
Dodecansure als
Zeckenrepellent
(Patentschrift).
TM II05 (Fr)
TM II05 (Fr)
TM II05 (Fr)
TM II05 (Fr)
TM II05 (Fr)
TM II05(Fr)
TM II05 (Fr)
TM II05 (Fr)
TM II05 (Fr)
TM II05 (Fr)
19
Dossier
12
Algal inhibition test
and antiseptics Quantitative suspension
test for the evaluation of
basic bactericidal
disinfectants and
1-5

recovery
Basic bacterial activity
against of a test material
against Staphylococcus
aureus (ATCC 6538) and
(ATCC 15442). A test
suspension of bacteria is
144
Communication
with UK (HSE)
Industry
organizaton
manufacturer
Communication
with UK (HSE)
REFERE
NCE
TITLE
PT
antiseptics - Test
method and
requirements (phase 1)
EN
113:1996/
A1:2004
Wood preservatives Test method for

determining the
against wood destroying
basidiomycetes Determination of the
toxic values
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
added to a prepared
sample of the product
under test. The mixture is
maintained at 20 oC. At a
specified contact time
following: 1 , 5, 15 , 30 , 45
or 60 minutes, an aliquot is
taken. The bactericidal
action of this aliquot is
immediately neutralised or
suppressed by a validated
method. The method of
choice is dilutionneutralisation. If a suitable
neutraliser cannot be
found, membrane filtration
is used. The number of
surviving bacteria in each
sample is determined and
the reduction in viable
counts calculated.A
criterion for activity by this
test method is that the test
material should
demonstrate at least a 5log reduction in viable
counts of the test
organisms in 60 minutes.
Laboratory test. Efficacy
criteria based on weight
loss of untreated specimen.
TYPE OF
REFERENCE
SOURCE
TM II05 (Fr)
TNsG PE
(updated)
TNsG on Prod
Eval
TM II05 (Fr)
NL guidance
UK guidelines
Test institute
Manufacturer
EN
117:2005
Wood preservatives Determination of toxic

values against
Reticulitermes species
(European termites)
(Laboratory method)
TNsG on Prod
Eval
TM II05 (Fr+De)
NL guidance
UK guidelines
145
REFERE
NCE
TITLE
PT
EN
118:2005

preventive action
against Reticulitermes
species (European
termites) (Laboratory
method)
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
TNsG on Prod
Eval
TM II05 (Fr+De)
NL guidance
UK guidelines
EN
12353:20
06
EN
12490:19
98
EN
1275:200
5
and antiseptics Preservation of test
organisms used for the
determination of
bactericidal,
mycobactericidal,
sporicidal and fungicidal
activity
solid wood Determination of the
penetration and
retention of creosote in
treated wood
basic fungicidal or basic
yeasticidal activity of
method and
1-5
General guidance
Industry
organizaton
TM II05 (Fr)
TNsG PE
1-5
TM II05 (Fr)
Basic fungicidal activity

against Candida albicans
(ATCC 10231) and
Aspergillus niger (ATCC
16404). A test suspension
of yeast cells or mould
spores is added to a
prepared sample of the
product under test. The
mixture is maintained at 20
oC. At a specified contact
time chosen from one of
the following 5, 15, 30 or 60
minutes, an aliquot is
taken; the fungicide action
in this portion is
immediately neutralised or
suppressed by a validated
method. The method of
choice is dilutionneutralisation. If a suitable
neutraliser cannot be
found, membrane filtration
is used. The number of
surviving yeast cells or
146
Industry
organizaton
Manufacturer
Communication
with UK (HSE)
TM II05 (Fr)
TNsG PE
REFERE
NCE
TITLE
PT
EN
1276:199
7
food, industrial,
domestic, and
institutional areas - Test
method and
requirements (phase 2,
step 1)
1-5
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
mould spores in each
sample is determined and
counts calculated.
The criterion for activity by
this test is that the test
material should
counts of the test
organisms in 60 minutes.
Dilution/Neutralisation .
Efficacy determined by a
reduction in CFU.
TYPE OF
REFERENCE
SOURCE
Industry
organizaton
Manufacturer
TM II05 (Fr)
TNsG PE
EN
12791:20
05
EN
13610:20
02
EN
13624:20
03
and antiseptics Surgical hand
disinfection - Test
method and
requirement (phase
2/step 2)
Chemical disinfectants Quantitative suspension
virucidal activity against
bacteriophages of
used in food and
industrial areas - Test
method and
step 1)
1-5
Testing mainly bactericidal

activity; in vivo test; phase
2 step 2
Industry
organizaton/
Manufacturer
TM II05 (Fr)
TNsG PE
Industry
organizaton
1-5
TM II05 (Fr)
TNsG PE
1-5
Industry
organizaton/man
ufacturer
147
REFERE
NCE
EN
13697:20
01
TITLE

method and
step 1)
and antiseptics Quantitative non-porous
surface test for the
evaluation of
bactericidal and/or
used in food, industrial,
domestic and
method and
requirements without
mechanical action
(phase 2/step2)
PT
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
TM II05 (Fr)
TNsG PE
1-5
Dilution/Neutralisation
Method. Efficacy measured
by a reduction in CFU.
Industry
organizaton
Manufacturers
TM II05 (Fr)
TNsG PE
EN
13704:20
02
EN
13727:20
03
Chemical disinfectants Quantitative suspension

sporicidal activity of
used in food, industrial,
domestic and
method and
step 1)
method and
step 1)
1-5
Industry
organizaton
Manufacturer
TM II05 (Fr)
TNsG PE
1-5
Efficacy determined by the

Industry
organizaton
Manufacturer
TM II05 (Fr)
148
REFERE
NCE
TITLE
PT
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
TNsG PE
EN
1390:200
6

eradicant action against
Hylotrupes bajulus
(Linnaeus) larvae Laboratory method
TNsG Prod Eval

TM II05 (Fr)
NL guidance
UK guidelines
EN
14204:20
04
EN
14347:20
05
mycobactericidal activity
of chemical
disinfectants and
method and
step 1)
and antiseptics - Basic
sporicidal activity - Test
method and
1-5
Method. Effiacy measured
by reduction in CFU.
Industry
organisation
Manufacturer
TM II05 (Fr)
TNsG PE
1-5
Basic sporicidal activity

against dormant spores of
Bacillus subtilis (ATCC
6633) and Bacillus cereus
(ATCC 12826). A prepared
sample of the product
under test is added to a
test suspension of bacterial
spores. The mixture is
maintained at 20 oC or any
other temperature to be
defined. At a specified
contact time chosen from
one of the following: 30, 60
and 120 minutes, an aliquot
portion is taken and the
sporicidal as well as
sporistatic action in this
portion is neutralised. The
method of choice is
dilution-neutralisation. The
number of surviving
bacterial spores is
determined in parallel and
counts calculated. The
effectiveness of
neutralisation is controlled
149
Industry
organizaton
Manufactuer
Communication
with UK (HSE)
TM II05
TNsG PE
REFERE
NCE
TITLE
PT
EN
14348:20
05
mycobactericidal activity
of chemical
disinfectants in the
medical area including
instrument disinfectants
- Test methods and
step 1)
and antiseptics Quantitative surface test
for the evaluation of
veterinary area on nonporous surfaces without
mechanical action Test method and
step 2)
1-5
and antiseptics Virucidal quantitative
suspension test for
human medicine - Test
method and
step 1)
1-5
EN
14349:20
07
EN
14476:20
05+A1:20
06
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
in the test.
The criterion for activity by
this test is that the test
material should
counts of the test
organisms in 120
minutes.Medical area
Veterinary area, Food,
industrial, domestic and
institutional hygiene
TYPE OF
REFERENCE
SOURCE
Industry
organizaton
Manufacturer
1-5
Non-porous surface test,

bactericidal, for
disinfectants used in the
veterinary applications
Method. By one
manufacturer, the test
material is deemed to have
passed the test and be
efficacious if it
demonstrates a log 4 or
more reduction in viable
counts under the conditions
defined in the test.
Reduction in viable
microbial counts compared
with water controls.
Industry
organizaton
Manufacturer
TM II05 (Fr)
TNsG PE
Industry
organizaton
Manufacturer
TM II05 (Fr)
150
REFERE
NCE
TITLE
PT
EN
14561:20
06
and antiseptics Quantitative carrier test
bactericidal activity for
instruments used in the
medical area - Test
method and
step 2)
fungicidal or yeasticidal
activity for instruments
used in the medical
area - Test method and
step 2)
virucidal activity of
the veterinary area Test method and
step 1)
Ophtalmic optics contact lense care
products - antimicrobial
preservative efficacy
testing and guidance on
determining discard rate
1-5
EN
14562:20
06
EN
14675:20
06
EN
14730:
2000
EN
1499:199
7
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
Industry
organizaton
Manufacturer
TM II05 (Fr)
1-5
Industry
organizaton
Manufacturer
TM II05 (Fr)
1-5
Industry
organizaton
Manufacturer
TM II05 (Fr)
1-5
Also ISO method.
Own searches
1-5
Industry
organizaton
Manufacturer
EN
1500:199
7
and antiseptics Hygienic handwash Test method and
requirements (phase
2/step 2)
and antiseptics Hygienic handrub - Test
method and
TM II05 (Fr)
TNsG PE
1-5
Industry
organizaton
Manufacturer
151
REFERE
NCE
TITLE
PT
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
requirements (phase
2/step 2)
TYPE OF
REFERENCE
SOURCE
TM II05 (Fr)
TNsG PE
EN 152-1
(1988)
EN 1522:1988/A
C1:1989
EN
15457:20
07
EN
15458:20
07
EN
1650:199
7
EN 1656 :
2000

determining the
preventive effectiveness
of a preservative
treatment against blue
stain in service - Part 1:
Brushing procedure

determining the
of a preservative
treatment against blue
stain in service - Part 2:
Application by methods
other than brushing
coating against fungi
coating against algae
food, industrial,
domestic, and
method and
step 1)
TNsG on Prod
Eval
TM II05 (Fr)
NL guidance
UK guidelines
TNsG on Prod
Eval
TM II05 (Fr)
NL guidance
UK guidelines
Manufacturer
Own searches
1-5
Industry
organizaton
Manufacturer
TM II05 (Fr)
TNsG PE
1-5
Method. Efficacy measured
by a reduction in CFU.
152
Manufacturers
REFERE
NCE
TITLE
PT
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
TYPE OF
REFERENCE
SOURCE

veterinary field - Test
method and
requirements (phase
2/step 1)
Industry
organisation
TM II05 (Fr)
TNsG PE
EN
1657:200
5/AC:200
7
EN 201:1992
EN 202:1993
fungicidal or yeasticidal
disinfectants and
method and
step 1)
1-5
By one manufacturer, the

product was to be deemed
to have passed the test if it
demonstrated a 10E5
logarithmic reduction in
viability.
By one manufacturer a
reduction in viable
microbial counts is
compared with water
controls.
The test material is
deemed to have passed
the test and be efficacious
if it demonstrates a log 4 or
more reduction in viable
counts under the conditions
defined in the test

against Lyctus
Brunneus (Stephens) Part 1: Application by
surface treatment
(laboratory method)

against Lyctus
brunneus (Stephens) Part 2: Application by
impregnation
Manufacturers
Industry
organizaton
TM II05 (Fr)
TNsG PE
TNsG on Prod
Eval
TM II05 (Fr)
NL guidance
UK guidelines
TNsG on Prod
Eval
TM II05 (Fr)
NL guidance
153
REFERE
NCE
TITLE
PT
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
(Laboratory method)
EN
252:1989/
AC1:1989

in ground contact
TYPE OF
REFERENCE
SOURCE
UK guidelines
8
Field test, in ground contact
TNsG on Prod
Eval
Test institute
NL guidance
UK guidelines
TM II05 (Fr)
EN
275:1992

against marine borers
Marine field test, 5 year

minumum
TNsG on Prod
Eval
Test institute
TM II05 (Fr)
NL guidance
UK guidelines
EN
330:1993

for use under a coating
and exposed out of
ground contact: L-joint
method
TNsG on Prod
Eval
Test institute
TM II05 (Fr)
NL guidance
UK guidelines
EN 3501:1994

TM II05 (Fr)
154
REFERE
NCE
EN 3502:1994
EN 3511:2007
EN 3512:2007
EN
370:1993
TITLE
Guide to the principles

of testing and
classification of the
natural durability of
wood
Guide to natural
durability and treatability
of selected wood
species of importance in
Europe
Classification of
preservative penetration
and retention
Guidance on sampling
for the analysis of
preservative-treated
wood
eradicant efficacy in
preventing emergence
of Anobium punctatum
(De Geer)
PT
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
TM II05 (Fr)
TM II05 (Fr)
TM II05 (Fr)
TNsG on Prod
Eval
TM II05 (Fr)
NL guidance
UK guidelines
EN
460:1994
EN 461:2005

solid wood - Guide to
the durability
requirements for wood
to be used in hazard
classes
preventive action
against Hylotrupes
TM II05 (Fr)
TNsG on Prod
Eval
TM II05 (Fr+De)
155
REFERE
NCE
TITLE
PT
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
bajulus (Linnaeus) Part 1: Larvicidial effect

(Laboratory method)
TYPE OF
REFERENCE
SOURCE
NL guidance
UK guidelines
EN 462:2006
EN
47:2005/A
C:2007

preventive action
against Hylotrupes
bajulus (Linnaeus) Part 2: Ovicidal effect
(laboratory method)
larvae of Hylotrupes
bajulus (Linnaeus) (Laboratory method)
TM II05 (De)
NL guidance
TNsG on Prod
Eval
TM II05 (Fr+De)
NL guidance
UK guidelines
EN
48:2005

eradicant action against
larvae of Anobium
punctatum (De Geer)
(laboratory method)
TNsG on Prod
Eval
TM II05 (Fr+De)
NL guidance
UK guidelines
EN 491:2005
EN 492:2005
EN

against Anobium
punctatum (De Geer) by
egg-laying and larval
survival - Part 1:
treatment (Laboratory
method)
against Anobium
punctatum (De Geer) by
egg-laying and larval
survival - Part 2:
Application by
impregnation
(Laboratory method)
Wood preservatives -
TNsG on Prod
Eval
TM II05 (Fr+De)
NL guidance
UK guidelines
TNsG on Prod
Eval
TM II05 (Fr+De)
NL guidance
UK guidelines
156
TNsG on Prod
REFERE
NCE
TITLE
73:1988/A
C:1992
Accelerated ageing of
biological testing Evaporative ageing
procedure
PT
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
Eval
TM II05 (Fr)
NL guidance
UK guidelines
EN
84:1997

biological testing Leaching procedure
TNsG on Prod
Eval
TM II05 (Fr)
NL guidance
UK guidelines
EN ISO
10253:20
06
EN ISO
117211:2001
EN ISO
117212:2003
EN ISO
20645:20
04
EN ISO
20743:20
Water quality - Marine

algal growth inhibition
test with Skeletonema
costatum and
Phaeodactylum
tricornutum (ISO
10253:2006)
Textiles - Determination
of the resistance of
cellulose-containing
textiles to microorganisms - Soil burial
test - Part 1:
Assessment of rotretardant finishing (ISO
11721-1:2001)
of the resistance of
cellulose-containing
textiles to microorganisms - Soil burial
test - Part 2:
Identification of longterm resistance of a rot
retardant finish (ISO
11721:2003)
Textile fabrics Determination of
antibacterial activity Agar diffusion plate test
(ISO 20645:2004)
of antibacterial activity
21
Growth inhibition test
TM II05
Manufacturer
Own searches
Manufacturer
Applied to textiles
157
Manufacturer
REFERE
NCE
TITLE
07
of antibacterial finished
products (ISO
20743:2007)
wood-based products Determination of
treatability of timber
species to be
impregnated with wood
preservatives Laboratory method
preventive efficacy
basidiomycetes fungi
contact - Horizontal lapjoint method
preventive effectiveness
against sapstain fungi
and mould fungi on
freshly sawn timber Field test
Wood preservatives Method for natural
preconditioning out of
ground contact of
treated wood
specimens prior to
biological laboratory test
wood-based products Wood-based panels Method of test for
determining the
resistance against
wood-destroying
basidiomycetes
wood-based products Assessment of the
effectiveness of a
masonry fungicide to
EN/TR
14734:20
04
EN/TR
14839:20
04
EN/TS
12037:20
03
EN/TS
15082:20
05
EN/TS
15397:20
06
ENV
12038:20
02
ENV
12404:19
97
PT
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
Reference material: non
treated polyester
TYPE OF
REFERENCE
SOURCE
Laboratory
Own searches
Own searches
Field test, out of ground

contact
Test institute
UK guidelines
Own searches
Own searches
TM II05 (Fr)
UK guidelines
10
Simulated use test
TNsG Prod Eval

TM II05 (Fr)
UK guidelines
158
REFERE
NCE
ENV
807:2001
TITLE
prevent growth into

wood of Dry Rot
Serpula lacrymans
(Schumacher ex Fries)
S.F. Gray - Laboratory
method
and other soil inhabiting
micro-organisms
PT
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
Laboratory test
TM II05 (Fr)
Test institute
NL guidance
UK guidelines
ENV
839:2002

basidiomycetes Application by surface
treatment
TM II05 (Fr)
NL guidance
UK guidelines
17
EPA 72-5
EPA 72-7
EPA/OPP
Protocol
Number:
1.201
Life-cycle test of fish

Simulated or actual field
testing for aquatic
organisms
Standard Norway Rat
and Roof Rat
Method
Communication
with UK (HSE)
Communication
with UK (HSE)
17
14
Anticoagulant rodenticide
test against Norway
rat/Roof rat applied as
liquid bait
TNsG on Prod
Eval
NL guidance
UK guidelines
EPA/OPP
Protocol
Number:
1.202
Standard House Mouse

Method
14
test against House mouse
applied as liquid bait
TNsG on Prod
Eval
NL guidance
UK guidelines
EPA/OPP
Protocol
Number:
1.203
Standard Norway Rat

and Roof Rat
Laboratory Test Method
14
test against Norway
rat/Roof rat applied as dry
bait
TNsG on Prod
Eval
NL guidance
UK guidelines
EPA/OPP
Protocol
Number:
1.204

14
applied as Dry bait
TNsG on Prod
Eval
NL guidance
159
REFERE
NCE
TITLE
PT
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
UK guidelines
EPA/OPP
Protocol
Number:
1.205
Standard Norway
Rat/Roof Rat
Powder Efficacy
14
test against Norway
Tracking powder
TNsG on Prod
Eval
NL guidance
UK guidelines
EPA/OPP
Protocol
Number:
1.207
Standard Norway
Rat/Roof Rat Acute
Test Method
14
Acute rodenticide test

applied as Liquid bait
TNsG on Prod
Eval
NL guidance
UK guidelines
EPA/OPP
Protocol
Number:
1.208

Acute Liquid Bait
14

against House mouse
applied as Liquid bait
TNsG on Prod
Eval
NL guidance
UK guidelines
EPA/OPP
Protocol
Number:
1.209
Standard Norway
Rat/Roof Rat Acute Dry
Method
14

applied as Dry bait
TNsG on Prod
Eval
NL guidance
UK guidelines
EPA/OPP
Protocol
Number:
1.210

Acute Dry Bait
14

against House mouse
applied as Dry bait
TNsG on Prod
Eval
NL guidance
UK guidelines
EPA/OPP
Protocol
Number:
1.211
Standard Norway
Rat/Roof Rat Acute
Tracking Powder
Efficacy Laboratory Test
Method
14

TNsG on Prod
Eval
NL guidance
UK guidelines
EPA/OPP
Protocol
Number:
1.212

Powder Efficacy
14
TNsG on Prod
Eval
NL guidance
UK guidelines
160
REFERE
NCE
TITLE
PT
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
EPA/OPP
Protocol
Number:
1.213
Standard Norway
Rat/Roof Rat
Anticoagulant Wax
14
test against Norway
rat/Roof rat applied as Wax
block and wax pellet
TNsG on Prod
Eval
NL guidance
UK guidelines
EPA/OPP
Protocol
Number:
1.214

Anticoagulant Wax
14
applied as Wax block and
wax pellet
TNsG on Prod
Eval
NL guidance
UK guidelines
EPA/OPP
Protocol
Number:
1.217
Standard Norway Rat

and Roof Rat
Anticoagulant
Placepack Dry Bait
14
test against Norway
Placepark dry bait
TNsG on Prod
Eval
NL guidance
UK guidelines
EPA/OPP
Protocol
Number:
1.218

Anticoagulant
14
applied as Placepark
penetration
TNsG on Prod
Eval
NL guidance
UK guidelines
EPA/OPP
Protocol
Number:
1.219
Standard Norway Rat

and Roof Rat Acute
14

applied as Placepark
penetration
TNsG on Prod
Eval
NL guidance
UK guidelines
EPA/OPP
Protocol
Number:
1.220

Acute Placepack Dry
Method
14

against House mouse
applied as Placepark dry
bait
TNsG on Prod
Eval
NL guidance
UK guidelines
EPA/OPP
Protocol
Number:
1.221
Proposed Norway Rat

Anticoagulant Technical
and Concentrated Dry
Method
14
test against Norway rat
applied as Technical and
TNsG on Prod
Eval
NL guidance
UK guidelines
EPA/OPP
Proposed Norway Rat
14
161
TNsG on Prod
REFERE
NCE
TITLE
Protocol
Number:
1.222
Acute Technical and

PT
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
against Norway rat applied
as Technical and
TYPE OF
REFERENCE
SOURCE
Eval
NL guidance
UK guidelines
EPA/OPP
Protocol
Number:
1.225
Proposed House Mouse

Anticoagulant Technical
and Concentrated Dry
Method
14
test against House mouse,
TNsG on Prod
Eval
NL guidance
UK guidelines
EPA/OPP
Protocol
Number:
1.226

Acute Technical and
14

against House mouse
TNsG on Prod
Eval
NL guidance
UK guidelines
EPA/OPP
Protocol
Number:
1.227

Acute Tracking Powder
Efficacy Laboratory Test
Method
14

against House mouse
TNsG on Prod
Eval
NL guidance
UK guidelines
EPPO
PP
1/203(1)
EPPO
PP
1/194(2)
(1995)
EPPO
PP
1/169(2
EPPO
PP
1/114(2)
EPPO
PP
1/201(1)
EPPO
PP
1/97(2)
EPPO
Admixture of plant
protection products to
stored plant products to
control insects and
mites
Blue-Stain fungi of
Softwood
Field rodents (Microtus,

Arvicola)
Field tests against
norvegicus, R. rattus)
Fumigants to control
insect and mite pests of
of rodenticidal dusts
Laboratory testing of
18 +
20
TM II05 (Fr)
Own searches
14
NL guidance
TM II05 (Fr)
14
NL guidance
TM II05 (Fr)
18 +
20
TM II05 (Fr)
14
UK guidelines
NL guidance
18
TM II05 (Fr)
UK guidelines
162
REFERE
NCE
TITLE
PT
PP
1/204(1)
plant protection
products against insect
and mite pests of stored
plant products
evaluation of the toxicity
and acceptability of
rodenticides and
rodenticide preparations
14
EPPO
PP
1/113(2)
EPPO
PP
1/197(1)
EPPO
PP
1/200(1)
EPPO
PP
1/199(1)
EPPO
PP
1/202(1)
EPPO
PP
1/198(1)
EPPO
(1982)
EPPO
(1982)
EPPO
(1986)
EPPO
Bulletin,
15 Pages
1-119,
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
NL guidance
TM II05 (Fr)
Test institute
14
Non-target effects of
rodenticides
Rodent repellents
against debarking of
trees
NL guidance
14
Own search
14
Own search
18
TM II05 (Fr)
Rodent seed repellents

Space and structural
treatments of store
rooms
Testing rodents for
resistance to
anticoagulant
rodenticides
Guidelines for the
rodenticides N1.
evaluation of the toxicity
and acceptability of
rodenticides and
rodenticide preparations
Guidelines for the
rodenticides. Field tests
against synanthropic
rodents (Mus musculus,
Rattus norvegicus,
rattus rattus)
Guidelines for the
rodenticides. Laboratory
and field tests for the
evaluation of
rodenticidal dusts.
The EPPO Conference
on Fumigation, Paris,
1983
UK guidelines
14
NL guidance
TM II05 (Fr)
14
TNsG on Prod
Eval
UK guidelines
Manufacturer
14
TNsG on Prod
Eval
UK guidelines
Manufacturer
14
TNsG on Prod
Eval
UK guidelines
18
TNsG on Prod
Eval
UK guidelines
163
REFERE
NCE
Paris
(1983)
EPPO,
Paris
(1982)
Fradin &
Day, July
2002
N Engl J
Med vol
347 vol
13-18
Hummel,
E.,
Kleeberg,
H. 1997.
in:
Practice
orientated
results on
use and
production
of NeemIngredient
s and
Pheromon
es V.
Proceedin
gs of the
5th
workshop,
Wetzlar,
Germany,
January
22-25,
1996
IBRG
(draft
MWF)
(1993)
ISO
TITLE
PT
EPPO
Recommendations on
fumigation standards
(2nd Edition)
Comparative efficacy of
insect repellents against
mosquito bites
18
Effect of the neem

extract formulation
neemazal-t/s on the
green pea aphid
acyrthosiphon pisum in
the laboratory (1995),
in: Practice orientated
results on use and
production of NeemIngredients and
Pheromones V
19
Dossier
A Standardized
Screening Method for
Determining the
Bioresistance of and
Evaluating Biocides in
Aqueous Metal Working
Fluids
13
TNsG on Prod
Eval
19
18
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
TNsG on Prod
Eval
Human subjects: Arm in

cage studies (15
volunteers, 10 mosquitoues
(Aedes aegypti) in each
cage. Endpoint: elapsed
time to first bite. Category
of protection A-H
(significantly different mean
complete protection time;
ANOVA & Tukey's). No
need to recalculate the
results to "real condition"
(simulate real condition)
Applicable to all textiles
164
UK guidelines
Dossier
TNsG on Prod
REFERE
NCE
TITLE
3998:197
7
of resistance to certain
insect pests
JIS K
1571:200
4
JIS L
1902:
2002
JIS Z
2801:
2000
JIS Z
2911:
1992 (PT
9)
MAFF
(1969)
MS 1004
part 1
(2002)
PT
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
containing animal fibres in
any proportion. Conditioned
voracity control specimens
and test specimens of
known mass are placed in
contact with selected larvae
for 14 days. The loss in
mass of all specimens and
the condition of the test
larvae are ascertained to
assess the resistance of
each test specimen.
Test methods for

determining the
effectiveness of wood
preservatives and their
performance
requirements
Testing for antibacterial
activity and efficacy
on textile products
Antimicrobial activity.
Reference material: e.g.
non treated polyester.
Antimicrobial Products
Test for antimicrobial
activity
and efficacy
Applied to textiles
Eval
TM II05 (Fr)
UK guidelines
Own searches
Test institute
Manufacturer
Manufacturer
Test institute
9
Methods of test for
fungus resistance
Disinfectants for use
specifically against: a)
anthrax, brucellosis,
contagious bovine
pleuro-pneumonia and
glanders; b) For use
against tuberculosis; c)
For use against footand-mouth disease; d)
For use against fowl
pest (Newcastle
disease fowl plague).
Specification for
mat: part 1: physical
and chemical
requirements (first
TYPE OF
REFERENCE
SOURCE
Applied to textiles
Manufacturer
1-5
TNsG PE
18
Manufacturer
165
REFERE
NCE
MS 1004
part 2
(2002)
MS 1004
part 3
(2002)
MS 1004
part 4
(2002)
MS 1008
(1986)
MS 1255
(1992)
MS 1257
(1992)
MS 1364
(1994)
MS 1398
part 1
(1996)
MS 1398
part 2
(1996)
MS 1398
part 3
(1996)
MS 1497
(2000)
TITLE
revision)
Specification for
efficacy - glass chamber
method (first revision)
Specification for
efficacy - glass cylinder
method
Specification for
efficacy - peet grady
method
Method for
determination of
delivery rate of aerosol
dispenser
Specification for
residual spray aerosol
Specification for
space spray aerosol
Method for
determination of brimful
capacity of aerosol cans
Specification for
vaporizer: Part 1:
requirement
Specification for
vapourizer: part 2:
method for evaluation of
biological efficacy glass chamber method
Specification for
vapourizer: part 3:
biological efficacy glass cylinder method
Methods of biological
evaluation of the
PT
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
18
Manufacturer
18
Manufacturer
18
Manufacturer
18
Manufacturer
18
Manufacturer
18
Manufacturer
18
Manufacturer
18
Manufacturer
18
Manufacturer
18
Manufacturer
18
Manufacturer
166
REFERE
NCE
MS 23
part 1
(1998)
MS 23
part 2
(1996)
MS 23
part 3
(1998)
NF B50100-4
October
2007
NF B50105-3
February
2008
NF FD
X40-501
November
2005
NF G39011 April
2001
NF T72050
Septembe
TITLE
efficacy of repellent bioassay method for

mosquito repellent on
human skin
Specification for
requirements (third
revision)
Specification for
biological efficacy glass chamber method
(first revision)
Specification for
biological efficacy - peet
grady method
wood-based products Definition of use classes
- Part 4 : national
declaration on the
situation of biological
agents
wood based products Preservative treated
solid wood - Part 3 :
wood preservation
performance and
treatment certificate Adaptation to France
metropolitan territory
and DOM
Protection - Termites Buildings protection
against termite
infestation
materials having
antiacarien properties Characterisation and
measurement of
antiacarien activity
Wood preservatives.
Technical grade borax.
Specifications and tests.
PT
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
18
Manufacturer
18
Manufacturer
18
Manufacturer
TM II05 (Fr)
TM II05 (Fr)
TM II05 (Fr)
18
Manufacturer
TM II05 (Fr)
TM II05 (Fr)
167
REFERE
NCE
r 1986
NF T72052
Septembe
r 1986
NF T72054
Septembe
r 1995
NF T72061
Septembe
r 1995
NF T72065
Septembe
r 1986
NF T72083/A1
November
1979
NF T72086
Septembe
r 1991
NF T72230
August
1988
NF T72281
Septembe
r 1986
NF T72320
March
1977
NF T72321
March
1977
TITLE
PT
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
Wood preservatives.
Boric acid.
TM II05 (Fr)
Wood preservatives.
Technical grade
pentachlorophenol.
Specificaitons and tests.
Wood preservatives.
Technical grade sodium
pentachlorophenate.
Wood preservatives.
Technical grade sodium
pentaborate.
Products for protecting
wood surfaces - Method
of testing resistance to
microorganisms
Wood preservatives.
Lasures. Natural
weathering test.
TM II05 (Fr)
TM II05 (Fr)
TM II05 (Fr)
TM II05
TM II05 (Fr)
Water-miscible,
neutralizable antiseptics
and disinfectants used
in liquid form.
Determination of
Dilution-neutralization
method.
Methods of airborne
disinfection of surfaces.
Determiation of
bactericidal, fongicidal
and sporicidal activity.
insects. Insecticide
distributed under
pressure ("aerosol"
type). Determination of
the efficiency rating.
1-5
Manufacturer
1-5
TM II05 (Fr)
18
TNsG on Prod
Eval

insects. Permanent
insecticide distributor.
efficiency rating and the
regularity rating.
18
TM II05 (Fr)
UK guidelines
TM II05 (Fr)
168
REFERE
NCE
TITLE
PT
NF X41513
August
1961
Protection of plastics.
Part 1. Testing method
for resistance of
ingredients to
microorganisms.
Protection of the plastic
materials - Part 3: Test
method of the
resistance of materials
and apparatus to
microorganisms
Protection of textiles.
Protection against
certain insect pests.
Methods of testing.
Protection. Testing
method for resistance of
paints to
microorganisms and
their protective power.
Protection of wood.
Testing methods for the
corrosive action of wood
protection products on
metals.
Wood preservatives.
against termites of
products designed for
masonry. Laboratory
method.
Wood preservatives.
Determination of
longicide efficacy of
temporary wood
protectives for green
sawn timber. Laboratory
method.
Wood preservatives.
Determination of
fongicide efficacy of
temporary wood
protectives for fresh cut
wood billets. Laboratory
method.
Wood preservatives.
TM II05
TM II05
18
TM II05 (Fr)
6+
7
TNsG on Prod
Eval
TMII05
TM II05 (Fr)
TNsG on Prod
Eval
NF X41515
March
1962
NF X41516
January
1980
NF X41520
March
1968
NF X41521 July
1968
NF X41541
Septembe
r 1994
NF X41547
December
1992
NF X41548
December
1992
NF X41555
August
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
TM II05
TNsG on Prod
Eval
TM II05
TM II05 (Fr)
TM II05 (Fr)
169
REFERE
NCE
TITLE
1982
{chaetomium}
{globosum} kunze. Soft
rotting agent.
Wood preservatives
Physicochemical testing
NF X41580 part
1-10
May 2006
NF XP
G39-010
May 2000
NF XP
X41-540
November
1995
NF XP
X41-540
November
1995
NF XP
X41-542
Septembe
r 1995
NF XP
X41-549
December
1999
NF XP
X41-549
December
1999
Not
available

surfaces having
antibacterial properties Characterisation and
measurement of
Wood preservatives.
Termites. Determination
of anti-termites action
for products used in
liquid phase for ground
method).
Wood preservatives.
Termites. Determination
of anti-termites action
for products used in
liquid phase for ground
method).
Wood preservatives.
Anti-termite treatment
product for floors, walls,
foundations and
masonry work.
Accelerated ageing test
of treated materials
prior to biological
testing. Percolation test.
Assessment of the
biocide efficacy using
PT
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
TM II05 (Fr)
Manufacturer
TM II05 (Fr)
TNsG on Prod
Eval
TM II05 (Fr)
TM II05 (Fr)
TNsG on Prod
Eval
1-5
product was mixed with
170
Manufacturer
REFERE
NCE
TITLE
PT
three algal species. Inhouse method
Not
available
Challenge Testing in
Metal Working Emulsion
(in-house laboratory
study)
13
Not
available
Disinfectant for
swimming pools for
control of bacteria, fungi
and algae.
Determination of
Minimum Inhibitory
Concentration Against
Bacteria, Fungi, Algae
and Cyanobacteria (in
house method)
1-5
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
algal suspensions
(Chlorella vulgaris
Navicula pelliculosa
Anabaena flos-aquae) in
glass conical flasks All
flasks were incubated and
shaken in an orbital shaker.
Samples were taken at 96h
and the chlorophyll a
concentration determined.
determining the EC50
value obtained for algae.
The purpose of the method
was to determine the
efficacy of preservatives
against bacterial and fungal
contamination in Metal
Working Fluids (MWF).
The method was conducted
by inoculating once a week
during 10 weeks mixed
bacteria and optionally
mixed fungi into water
based MWF emulsions
containing various amounts
of the test substance
diluted in 3% synthetic oil.
After each week a sample
of the emulsion was plated.
After plate incubation the
number of surviving
organisms was compared
to the control The criteria
for measuring good
microbial count of <10
cfu/ml after 10 inoculations
for all microorganisms.
Using a growth inhibition
test method for recording
MIC values to test the
efficacy of the product at
various diluted
concentrations. MIC value
was determined using
microdilution technique in
microtiter plates
Tests were carried out in
quadruplicate. The ppm
level of the product in the
171
TYPE OF
REFERENCE
SOURCE
Manufacturer
Manufacturer
REFERE
NCE
TITLE
PT
Not
available
of waste water. A
laboratory study
involving disinfection of
untreated river water, as
a surrogate for a full
field trial. (in-house
methods)
of waste water. Full
scale field trial
1-5
Not
available
of waste water.
Inactivation of enteric
micro-organisms in
tertiary treated
municipal waste waters
(in-house methods)
1-5
Not
available
of waste water. Pilot
studies to assess the
disinfection
performance of on a
physicochemical
wastewater effluent.
1-5
Not
available
1-5
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
last microtiter well
demonstrating no growth
was determined as the MIC
for the product against that
microorganism.
Several concentrations of
the product were added to
river water and allowed to
react for 30 mins. Total
counts and coliforms were
determined. Efficacy was
determined based on
reduction in CFU counts.
A product was used to treat
waste water at 3
concentrations under real
field conditions involving a
waste water treatment
plant; variable flow and
bacterial load, and
operating temperatures.
Efficacy was measured
based on a log removal of
bacteria after 15 and 30
mins.
Experiments were
conducted to investigate in
pilot scale the disinfection
efficiency against enteric
micro-organisms in tertiary
treatment waste waters and
the effect of dose and
contact time on disinfection
efficiency. After incubation
bacterial colonies were
counted and microbial
numbers calculated as cfu/
100 ml.
percentage reductions of
microorganisms after 8 and
18 minutes contact time.
A purpose built disinfection,
continuous flow, Pilot Plant
was specially constructed
and installed at a
functioning wastewater
treatment plant. Prior to
installation the wastewater
was treated only by
chemically assisted
172
TYPE OF
REFERENCE
SOURCE
Manufacturer
Manufacturer
Manufacturer
Manufacturer
REFERE
NCE
TITLE
PT
Not
available
Field Evaluation of in a
Termite Control Baiting
System
18
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
sedimentation, making
disinfection particularly
difficult. As part of this pilot
study, modifications to the
Plant and process were
explored and adopted on
the basis of experience and
experimental data using
standard methodologies.
Disinfection efficiency
under a number of
conditions and wastewater
loads were explored.
Method for recording
faecal coliforms: American
Public Health association;
American Water Works
Association and Water
Environment federation
(1998) Standard Methods
for the Examination of
20th Ed.; Washington DC,
USA; Method 9222D.
Method for recording
Enterococcus, coliphage
Clostridium
according to laboratory
methods. Efficacy was
determined by measured
Log reductions (or removal)
in counts. Colonies were
counted before and after
disinfection.
Structures in this study
were supplied by
cooperating pest control
companies. A number of
the structures had been
identified as "problem
houses" by the pest control
operator.
Stations were inspected at
approximately monthly
(when active), and bimonthly (when not active)
intervals for termite activity,
with the condition of the
station (active, inactive and
baited) being noted on an
inspection form. When
173
TYPE OF
REFERENCE
SOURCE
Manufacturer
REFERE
NCE
TITLE
PT
Not
available
Field trial in a Plant

recirculating cooling
system
11
Not
available
In house Laboratory
Study of the Efficacy
fIavipes
18
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
activity was found in a
station, bait was placed in
the station.
The thin wood strips of the
baiting station were fan
sprayed with product
solution using a
compressed air sprayer.
Product was mixed in the
acetone such that when the
solution was applied to the
strips, various
concentrations of product
were imparted to the wood
strips .A tumble technique
was employed to prevent
uneven concentrations of
active ingredient in the
sawdust matrix. Efficacy
was measured by
infestation
The antimicrobial
effectiveness of biocide
was determined by
monitoring chemical and
physical cooling water
characteristics,
microbiological parameters
such as viable counts of
bacteria, fungi and algae,
and operational and
engineering parameters of
the tested cooling system
Analysis methods used for
microbial counting were
from:
Standard methods for the
Waste Water (APHA 1992),
and in-house Laboratory
SOPs. Efficacy was
determined by a reduction
in microbial counts
Formulation, applied as a
residual spray, was
assessed for efficacy
against cockroaches (Blatta
orientalis, Blatella
americana) under
174
TYPE OF
REFERENCE
SOURCE
Manufacturer
Manufacturer
REFERE
NCE
TITLE
PT
Not
available
Insecticidal activity of an
oil based aerosol
against German
cockroach, Blattella
germanica (in-house
laboratory study)
18
Not
available
against American
cockroach, Periplaneta
Americana
18
Not
available
against Housefly,
Musca domestica.
18
Not
available
against mosquito, Culex
pipiens pallens
18
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
laboratory conditions. The
spray deposits were
assessed at intervals up to
24 weeks post application.
Efficacy of the formulation
at each interval, in terms of
knockdown and mortality,
was evaluated over a
period of 96 hours post
treatment application
Efficacy was measured
when all termites in a
container had died, the
length of time of
consumption by the
termites of the treated
sawdust was recorded
10 German cockroaches
(Blattella germanica) were
released into a plastic
container within a glass
cylinder and the aerosol
was sprayed into the
cylinder. Knocked down
6 adult American
cockroaches (Periplaneta
Americana) were released
into plastic container which
was placed in the centre at
the bottom of the glass
cylinder and 1013mg of
aerosol was sprayed into
the cylinder and was then
covered by a glass lid.
Knocked down insects
were counted at intervals
up to 20 minutes.
100 houseflies were
released into a Peet-Grady
chamber and aerosol was
sprayed into the chamber.
up to 20 minutes.
50 mosquitoes (Culex
pipiens pallens) were
released into a Peet-Grady
chamber and aerosol was
sprayed into the chamber.
175
TYPE OF
REFERENCE
SOURCE
Manufacturer
Manufacturer
Manufacturer
Manufacturer
REFERE
NCE
TITLE
PT
Not
available
Insecticide primarily for

the control of termites:
Laboratory Study
fIavipes
18
Not
available
Laboratory studies to
assess products applied
as direct sprays for
efficacy against a range
of crawling insects
18
Not
available
Other in can
preservatives. In-house
method based on
Antimicrobial
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
up to 20 minutes. The
chamber was then
ventilated by an exhaust
fan and all insects were
transferred to a clean
recovery container with diet
and water within 20
minutes
product were impregnated
in a sawdust matrix readily
consumed by termites.
Product was incorporated
into the sawdust by placing
each batch of solution and
one batch of sawdust in the
mixing bowl of a Kitchenaid
Model K5SS mixer. One
untreated batch of sawdust
served as a control.
Sterile sand and distilled
water was mixed to form a
moistened sand substrate
to sustain the termites as
they fed upon the samples.
Eight replications of each
concentration plus the
control were tested.
Termite activity was
observed in each of the
containers for four days.
infestation.
Product was applied as a
direct spray, was assessed
for efficacy against
cockroaches (Blatta
orientalis, Blatella
americana) under
laboratory conditions.
Efficacy criteria based on
mortality and knocked
down insects
by inoculating mixed
bacteria and mixed fungi
into a interior paint
176
TYPE OF
REFERENCE
SOURCE
Manufacturer
Manufacturer
Manufacturer
REFERE
NCE
TITLE
PT
Preservative
Effectiveness Challenge
Test and DAB 10
guidelines
Not
available
Preservative for
detergents. In-house
method based on
Antimicrobial
Preservative
Effectiveness Challenge
Test and DAB 10
guidelines
Not
available
Repellency of Two
Formulations against
Ants (in-house
laboratory study)
19
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
formulation containing
various concentrations of
the active substance which
was repeated on a weekly
basis. After 0, 7 14, 21 and
28 days samples of the
formulations were placed in
a preservative neutralizer
compared to the control.
good preservation efficacy
was a microbial count of
<10 cfu/ml at day 7, 14 , 21
and 28 days
by inoculating mixed
bacteria and mixed fungi
into a washing up liquid
formulation containing
various concentrations of
the active substance which
was repeated on a weekly
basis. After 0, 7 14, 21 and
28 days samples of the
formulations were placed in
a preservative neutralizer
compared to the control .
good preservation efficacy
was a microbial count of
<10 cfu/ml at day 7, 14 , 21
and 28 days.
Vinyl floor tiles were
sprayed with the repellent
solution and placed in
cages. A food attractant
was placed in the centre of
each tile. The number of
ants crossing each tile was
counted at 30 and 60
minutes after tiles were
placed into cages. Data for
30 and 60 min was
combined and averaged.
Repellency was also
177
TYPE OF
REFERENCE
SOURCE
Manufacturer
Manufacturer
REFERE
NCE
TITLE
PT
Not
available
Repellency of Two
product formulations
against the German
Cockroach (in-house
laboratory study)
19
Not
available
Swimming pool
disinfectant:
Residential spa field
test
1-5
NWPC Standard for

testing of wood
preservatives.
Mycological test.
"Jordburk" method - A
soil block test with
wood-rotting
Basidiomycetes.
NWPC Standard for
testing of wood
preservatives.
Mycological test.
"Mullde" method - A
soil block test in
unsterile soil.
Mycological testing of
anti-stain preservatives
for freshly sawn timber.
Test institute
Test institute
Test institute
NWPC
1.4.1.1./7
0
NWPC
Standard
1.4.1.2./7
0
NWPC
Standard
1.4.1.3./7
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
measured the day after the
fourth consecutive
application. Sufficient
>65%.
The cockroaches had a
choice of two shelters, one
treated with the product
and one untreated.
Repellency after 1, 3, 7
and 14 days was recorded.
Percent repellency was
calculated. Sufficient
>65%, excellent repellency
was defined as >85%.
A residential spa was used
to test the efficacy of the
product in typical field
conditions. The
maintenance pattern used
was considered to be
typical of the average spa
owner, i.e. conforming to
minimum and not optimum
use pattern and
maintenance procedures.
Bacteriological analysis
was conducted on 150
samples were taken over
the 90 day test period.
Efficacy was determined by
a reduction in cfu/100ml.
178
TYPE OF
REFERENCE
SOURCE
Manufacturer
Manufacturer
REFERE
NCE
TITLE
The Miniboard method.

(Mykologisk provning av
trskyddsmedel mot
blnad och mgel p
nysgat virke.
Minibrdmetoden.)
wood preservatives
Mycological test. Field
test - A field test with
stakes.
wood preservatives
Marine test - A test
against marine
woodboring organisms
in sea water.
Proposed development
of an OECD Guidance
Document for
establishing the efficacy
of biocides used in
swimming pools and
spas
Update on work to
validate efficacy
methods for
antimicrobials used on
hard surfaces
Guidance document on
the evaluation of the
efficacy of antimicrobial
treated articles with
claims for external
effects
NWPC
Standard
1.4.2.1./7
1
NWPC
Standard
1.4.2.2./7
3
OECD
(ENV/JM/
BCID(200
7)2)
OECD
(ENV/JM/
BCID(200
7)3)
OECD
(ENV/JM/
BCID(200
7)5)
OECD
203
(1992)
OECD
204
(1984)
OPPTS
810.3000
(1999)
PT
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
Test institute
Test institute
1-5
Own searches
10
Own searches
Own searches
17
Communication
with UK (HSE)
17
Communication
with UK (HSE)
Fish, Acute toxicity test

Fish, Prolonged toxicity
test: 14-day study
General Considerations
for Efficacy of
Invertebrate Control
Agents
18
General guide
Manufacturer
UK guidelines
179
REFERE
NCE
TITLE
PT
OPPTS
810.3100
OPPTS
810.3200
Soil treatments for

imported fire ants
Livestock, poultry, furand wool-bearing
animal treatments
Treatments to control
pests of humans and
pets
18
Own searches
18
Own searches
18
UK guidelines
OPPTS
810.3400
Mosquito, black fly, and

biting midge (sand fly)
treatments
18
OPPTS
810.3500
Premises treatments
18
OPPTS
810.3300
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)

flying insects: Mosquito
Black Fly
Biting Midge (Sand Fly)
General guideline
TYPE OF
REFERENCE
SOURCE
UK guidelines
Manufacturer
UK guidelines
OPPTS
810.3700
public
draft
OPPTS
810.3800
Powers et
al. Applied
and
Environm
ental
Microbiolo
gy, 60:
2316
2323
(1994)
Insect Repellents For

Human Skin and
Outdoor Premises
19
Own search
Methods for efficacy

testing of termite baits
Biocidal Efficacy of a
Flocculating Emergency
Water Purification
Tablet.
18
Own searches
prEN
13623
bactericidal activity
against Legionella
pneumophila of
for aqueous systems Test method and
step 1)
1-5
1-5
Biocidal emergency water

purification tablets were
tested for bactericidal,
virucidal and cysticidal
efficacy in water at
temperatures ranging from
5 to 25C to validate and
verify the effectiveness of
the tablets for the
destruction and removal of
microorganisms. Counts of
surviving organisms were
compared with the initial
counts to calculate the logreduction. Replicate
results were averaged
Manufacturer
Own searches
180
REFERE
NCE
TITLE
PT
prEN
14563
mycobactericidal or
tuberculocidal activity of
used for instruments in
method and
step 2)
and antiseptics Application of European
Standards for chemical
disinfectants and
antiseptics
1-5
prEN
14885:20
06
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
Manufacturer
TM II05 (Fr)
1-5
Industry
organizaton
Manufacturer
TM II05 (Fr)
prEN 807
rev
Rawlinson
and
Shennan,
1987.
RENAULT
D551721
(1987)
Report
EPS
1/RM/25
Environm
ent
Canada.
1992
SABS
1102
(1987)
SABS

and other soil inhabiting
micro-organisms
A recirculating test rig
for the investigation of
metal-working fluid
spoilage. In Industrial
microbiological testing.
Edited by Hopton and
Hill, Blackwell Scientific
Publications, Oxford.
ISBN 0 632 01793 7. pp
227-231
Evaluation of the
Biostability of Aqueous
Metal Working Fluids
Biological Test Method:
Growth Inhibition Test
Using the Freshwater
Alga Selenastrum
capriconutum
Own searches
13
TNsG on Prod
Eval
13
TNsG on Prod
Eval
Bacterial efficacy of
biocides used in waterbased emulsion paints
South African standard
12
Efficacy test against Alga:

Selenastrum capriconutum
Communication
with UK (HSE)

recovery
Efficacy test for in can

preservatives in paints
(emulsion) against bacteria.
13
TNsG on Prod
Eval
TNsG on Prod
181
REFERE
NCE
TITLE
14351987
specification for
biocides for use in
emulsions of aqueous
metal working fluid and
aqueous hydraulic fluid.
Pesticides: Biological
evaluation of mists and
fogs - first revision
Pesticides Rearing
and handling of the
human body louse
(Pediculus humanus
humanus L.) - first
revision
Pesticides Rearing
and handling of the
common clothes moth
(Tineola bisseliella
Hummel) - second
revision
Pesticides Rearing
and handling of the
German cockroach
(Blatella germanica (L.))
- second revision
evaluation of
insecticidal oil-based
space spray in lowpressurized dispensers
- first revision
evaluation of the
contact efficacy of liquid
residual insecticides first revision
evaluation of materials
that release an
insetticide upon heating
evaluation of knockdown and killing
proprieties of liquid and
aerosol formulation (al
posto di Standard
methods SABS Method
8689-first revision)
Pesticides: biological
evaluation of the
proprerties of solid fly
SABS 233
st
1 rev
SABS 303
SABS 332
SABS 458
SABS 576
SABS 583
SABS
6136
(2003)
SABS 689
rd
3 ed
(2002)
SABS 690
(DRAFT)
PT
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
Eval
18
Manufacturer
18
Manufacturer
18
Manufacturer
18
Manufacturer
18
Manufacturer
18
Manufacturer
18
Manufacturer
18
Manufacturer
18
Manufacturer
182
REFERE
NCE
SABS 695
SABS 807
TITLE
baits - DRAFT
evaluation of the
efficacy of mosquito
repellents - first
revision
Methods for testing
insecticides against
flying and crawling
insects.
PT
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
TYPE OF
REFERENCE
SOURCE
19
Manufacturer
18
TNsG on Prod
Eval
Manufacturer
UK guidelines
SABS 899
(1987)
Insecticidal space spray

in pressurized
dispensers
S&M Boko test
18
SM020 (in
house)
S&M Fako test
SM021 (in
house)
S&M KOKO test
SM019 (in
house)
11
Manufacturer
Preservation of water
diluted coolants.
preservatives, inoculated
and streaked on agar
plates.
Test germ: bacteria,
moulds, Yeasts
4 evaluation levels. 12
cycles.
Preserving effect of
chemical preservatives in
water containing coatings,
adhesives and other water
containing technical
emulsions or dispersions
(in can).
plates.
Yeasts
"Well preserved" if after 6w
no microbial growth can be
observed.
Corresponds to 2 years of
4 evaluation levels
Preservatives in cosmetic
formulations, e g creams,
183
Manufacturer
Manufacturer
Manufacturer
REFERE
NCE
TITLE
PT
SM022 (in
house)
Determining the
growth
10
SM022a
(in house)
Determining the
growth
10
SM023 (in
house)
Determining the
resistance to algal
growth
10
SM026 (in
house)
S&M Betoko test:

SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
shampoos etc (in can)
plates.
Yeasts
"Well preserved" if after 6w
no microbial growth can be
observed.
Corresponds to 30 months
of microbial stability.
4 evaluation levels
coatings on paper
Test germ: Aspergillus
niger and Penicillum
funiculosum
"Sufficiently finished
against fungal growth" if
max 1% growth.
coatings on paper
Test germ: Aspergillus
niger and Penicillum
funiculosum
"Sufficiently finished
against fungal growth" if
max 1% growth.
coatings to algal attack.
Test substrate: masonry
coatings on paper.
Test germ: Scenedesmus
vacuolatus.
No algal growth on the test
pieces after 2 weeks:
"Effectively protected
against algal growth"
concrete admixtures (in can
184
TYPE OF
REFERENCE
SOURCE
Manufacturer
Manufacturer
Manufacturer
Manufacturer
REFERE
NCE
TITLE
PT
in concrete admixtures
SM029 (in
house)
S&M FeuTuKo test
SM036 (in
house)
S&M WiWako test
SM037 (in
house)
S&M TaBaKo test
SM044 (in
house)
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
preservation).
plates.
Yeasts
Free of growth during 4
weeks: "Well preserved"
Corresponds to 1 year of
microbiological stability
Preservatives in wet
tissues in their original
packaging.
Inoculation of whole
packaging and after 3w of
incubation, pieces of tissue
is placed on agar plates.
Yeasts
3d bacteria, 7d moulds. 4
evaluation levels.
Preservation of fountain
solutions for offset printing.
plates.
Yeasts
12 cycles
Preservation of paint baths.
plates.
Yeasts
12 cycles. 4 evaluation
levels.
185
TYPE OF
REFERENCE
SOURCE
Manufacturer
Manufacturer
Manufacturer
Manufacturer
REFERE
NCE
TITLE
PT
Antibacterial Activity,
Test
Antimycotic Activity
Test
antibacterial activity,
germ count method
Cutting fluid, soluble,
biostable joint service
designation ZX-9
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
household formulations, e g
softener, washing up
liquids, all purpose cleaner
(in can preservation).
plates.
Yeasts
Free of growth during 6
weeks: "Well preserved". (4
evaluation levels).
Corresponds to 30 months
of microbiological stability
Applied to textiles
Applied to textiles
Manufacturer
Applied to textiles
Manufacturer
Test method for textiles

to determine resistance
to insects (e.g. moths,
carpet beetles)
18
Test method for aerosol

space sprays against
flying insects
18
in household
formulations
SN 195
920 1994
SN 195
921 1994
SN 195
924 1983
UK MOD
91-70
issue
(1990)
US
AATCC
Technical
Manual
Method
24 (1992)
US CSMA
Aerosol
Guide
7 th
Edition,
pages
129-134
(1981)
US CSMA
Aerosol
Guide
7 th
Edition,
13
TYPE OF
REFERENCE
SOURCE
Manufacturer
TNsG on Prod
Eval
Efficacy test against larvae
TNsG on Prod
Eval
UK guidelines

flying insects:
TNsG on Prod
Eval
UK guidelines
Manufacturer
Test method for

pressurised spray
products against
cockroaches
18

crawling insects:
cockroaches
TNsG on Prod
Eval
UK guidelines
186
REFERE
NCE
pages
135-139
(1991)
US EPA
72-1
US EPA
96-2
US EPA
96-5
US EPA
96-7
US EPA
Guideline
OPPTS
810.3700
(1999)
Verwey &
Sosa,
2007
TITLE
PT
Acute toxicity test for

freshwater fish
17
17
15
Avian Toxicants
Avian Frightening
Agents
Insect repellents for
human skin and outdoor
premises
15
19
18
WHO/CD
S/WHOP
ES/GCDP
P/2003.5
Space spray application

of insecticides for vector
and public health pest
control a practitioners
guide
18
WHO/VB
C/75.593
(1981)
Instructions for
determining the
susceptibility or
resistance of
cockroaches to
insecticides
Instructions for
determining the
susceptibility or
18
TYPE OF
REFERENCE
SOURCE
Communication
with UK (HSE)
Communication
with UK (HSE)
TNsG on Prod
Eval
TNsG on Prod
Eval
UK guidelines
Fish control agents
Liquid Electric test

method
WHO/VB
C/81.212
(1981)
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
For testing pyrethroids

(draft method) and natural
actives (Pyrethrum extract)
on mosquitoues
(knockdown). Efficacy
criteria: "effective against
mosquitoes for X hours".
Knockdown is measured
repeatedly for 2h and
mortality after 24h. Control
(no treatment) knockdown:
maximum 10%. 2-4
chamber replicates, 50
organisms in each. Mean
and Standard Deviations
for each time calculated as
well as KT50 and KT80
(Mean time to 50% and
80% knockdown
respectively).
Brief description of the
main types of space spray
equipment as well as the
operational guidelines for
space spray application of
insecticides.
Manufacturer
TM II05 (Fr)
TNsG on Prod
Eval
UK guidelines
18
TNsG on Prod
Eval
187
REFERE
NCE
WHO/VB
C/81.805
WHO/VB
C/81.806
WHO/VB
C/81.807
(1981)
WHO/VB
C/81.808
(1981)
WHO/VB
C/81.809
(1981)
WHO/VB
C/81.810
(1981)
WHO/VB
C/81.811
(1981)
WHO/VB
C/81.812
TITLE
larvae to insect
Instructions for
determining the
susceptibility or
resistance of adult
mosquitoes to
organochlorine,
organophosphate and
carbamate insecticides,
- establishment of the
baseline.
Instructions for
determining the
susceptibility or
resistance of adult
mosquitoes to
organochlorine,
organophosphate and
carbamate insecticides diagnostic test
Instructions for
determining the
susceptibility or
Instructions for
determining the
susceptibility or
resistance of body or
headlice to insecticides
Instructions for
determining the
susceptibility or
resistance of adult bedbugs to insecticides
Instructions for
determining the
susceptibility or
resistance of adult
blackflies, sandflies and
biting midges to
insecticides
Instructions for
determining the
susceptibility or
resistance of blackfly
Instructions for
determining the
PT
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
18
TYPE OF
REFERENCE
SOURCE
TNsG on Prod
Eval
UK guidelines
18
TNsG on Prod
Eval
UK guidelines
18
TNsG on Prod
Eval
UK guidelines
18
TNsG on Prod
Eval
18
TNsG on Prod
Eval
UK guidelines
18
TNsG on Prod
Eval
UK guidelines
18
TNsG on Prod
Eval
UK guidelines
18
UK guidelines
188
REFERE
NCE
TITLE
(1981)
susceptibility or
larvae to insect
Instructions for
determining the
susceptibility or
resistance of houseflies,
tsetse flies, stableflies,
blowflies etc. to
insecticides
Instructions for
determining the
susceptibility or
resistance of adult ticks
to insecticides
Instructions for
determining the
susceptibility or
resistance of fleas to
insecticides
WHO/VB
C/81.813
(1981)
WHO/VB
C/81.814
(1981)
WHO/VB
C/81.815
(1981)
PT
SHORT TEST
METHOD AVAILABLE OR
INFORMATION
PROVIDED FROM
ELSEWHERE)
18
TYPE OF
REFERENCE
SOURCE
TNsG on Prod
Eval
UK guidelines
18
TNsG on Prod
Eval
UK guidelines
18
TNsG on Prod
Eval
UK guidelines
189

Efficacy Testing of Biocidal Products - Overview of Available Tests - ASW - FBE - 080305 - Final PDF

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October 2012

Mall-id: MAG-0006, 2011-12-28

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ACRONYMS AND ABBREVIATIONS

Web page to organisations (if available)

Efficacy of a biocidal product can be defined as the power to produce an effect,

ACRONYMS AND ABBREVIATIONS ................................................................. 1

BACKGROUND AND AIM OF THE PRESENT OVERVIEW ............................... 6

Biocidal Products Directive .............................................................................. 6

Data requirements related to efficacy .............................................................. 6

AIMS OF THIS OVERVIEW ................................................................................ 8

PERFORMANCE STANDARDS vs LABEL CLAIMS ....................................... 10

EXPERIMENTAL DESIGN OF EFFICACY TESTS ........................................... 10

STANDARDISED AND "IN HOUSE" PROCEDURES ...................................... 11

PT 6: In-can preservatives .............................................................................. 12

PT 8: Wood preservatives ............................................................................... 13

PT 10: Masonry biocides ................................................................................. 14

PT 13: Metalworking fluid preservatives ........................................................ 14

PT 14: Rodenticides ........................................................................................ 14

PT 18: Insecticides, acaricides and products to control other

PT 21: Antifouling products ............................................................................ 15

TEST REFERENCES FOUND IN THIS OVERVIEW ......................................... 15

International standardisation organisations own searches ...................... 16

Tests used in other MSs TM and national guidelines ................................ 17

Methods in use according to manufacturers and industry

Test endpoints ................................................................................................. 19

LIMITATIONS AND KNOWLEDGE GAPS ........................................................ 19

EVALUATING EFFICACY COMPARING TEST RESULTS TO

CONCLUSIONS AND SUGGESTIONS............................................................. 22

APPENDIX I. TEST PROCEDURES (STANDARDS AND OTHER

BACKGROUND AND AIM OF THE PRESENT OVERVIEW

Biocidal Products Directive

In the European Community, authorisation of Biocidal Products (BP) is regulated

Data requirements related to efficacy

In an OECD survey from 1999, some questions on how authorities regarded

The 23 different Product Types (PTs) are described in Annex V of BPD.

In chapter 7 of the TNsG on Product Evaluation, there is some guidance on how to

AIMS OF THIS OVERVIEW

PERFORMANCE STANDARDS vs LABEL CLAIMS

Performance standards refer to a predetermined efficacy required by the

EXPERIMENTAL DESIGN OF EFFICACY TESTS

The product should preferably be tested at a variety of application rates, including

STANDARDISED AND "IN HOUSE" PROCEDURES

from CEN, AOAC/EPA, ASTM and MAFF

microorganisms causing aesthetic problems (odour) in presence of

Spoilage of in-can and in-tank products may be the result of

o The level of active substance is usually measured by a suitable

PT 10: Masonry biocides

Effectiveness (often described as kill or prevention of regrowth) depends on

"untreated controls" and to design field tests.

PT 13: Metalworking fluid preservatives

Simple laboratory tests generally determine MIC (Minimum Inhibitory

Rodent species can cause montory loss (through consumption or spoilage

PT 18: Insecticides, acaricides and products to control other arthropods

PT 21: Antifouling products

Although there are a few standards related to antifouling products, these

TEST REFERENCES FOUND IN THIS OVERVIEW

available tests (see next section). Manufacturers, industry organisations, other

International standardisation organisations own searches

and applications), G03.04 (Weathering and durability, biological deterioration),