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Original article
Division of Pediatric
Cardiology, University of Bonn,
Bonn, Germany
2
Division of Neonatology,
University of Bonn, Bonn,
Germany
3
Department of Obstetrics and
Fetal Medicine, University
Medical Center HamburgEppendorf, Hamburg, Germany
Correspondence to
Dr Ulrike Herberg, Division of
Pediatric Cardiology, University
of Bonn, Adenauerallee 119,
Bonn D 53113, Germany;
Ulrike.Herberg@ukb.uni-bonn.de
Received 8 August 2013
Revised 1 May 2014
Accepted 31 May 2014
Published Online First
27 June 2014
ABSTRACT
Background In twin-to-twin transfusion syndrome
(TTTS), genetically identical twins are exposed to
different haemodynamic conditions during fetal life,
which are considered to be the cause of prenatal and
postnatal cardiovascular differences between the donor
and the recipient.
Objective To assess intertwin differences on childhood
cardiac outcome after intrauterine laser coagulation
therapy (LC) of severe TTTS.
Design and patients Prospective, detailed,
echocardiographic follow-up of 31 twin pairs aged 9.95
0.8 years (meanSD) with severe TTTS treated by LC,
and the comparison with reference values.
Results Cardiac function was normal and did not show
intertwin differences in twins without structural heart
disease. Discordant birth weight or birth weight <3rd
centile for gestational age had no inuence on blood
pressure and cardiac indices. Pulmonary stenosis was
more common (5/62; 8.1%) than in the general
population ( prevalence 0.066%, relative risk 134.4,
95% CI 42.1 to 428.8, p<0.0001) and affected both
donor and recipient. Intertwin differences in late diastolic
right ventricular lling ( peak velocities: recipient 0.51
0.11 m/s vs donor 0.450.10 m/s, mean difference
0.74 m/s, 95% CI 0.23 to 1.24, p=0.009) and early
septal relaxation (mean myocardial velocities: recipient
8.21.5 cm/s vs donor 8.91.2 cm/s, mean
difference 0.7 cm/s, 95% CI 0.02 to 1.38, p=0.044)
were found only when twins with right heart disease
were included.
Conclusions Despite severe prenatal cardiac
involvement, childhood cardiac function is normal in the
majority of surviving donors and recipients after
successful LC of severe TTTS. This underlines the
favourable impact of intrauterine LC on postnatal
cardiovascular performance.
INTRODUCTION
Placental vascular anastomoses provide the anatomical basis for the unbalanced intertwin transfusion from donor to recipient twin.1 3 In the
recipient, an increase in preload and afterload
results in systemic hypertension, myocardial hypertrophy, diastolic dysfunction and progressive
cardiac failure.3 6 7 Besides functional cardiac
impairment, right ventricular outow tract obstruction (RVOTO) has been reported in recipients and
is attributed to increased loading of the fetal right
ventricle (RV).810 In contrast, the smaller donor
shows less cardiac pathology but manifests moderate right ventricular diastolic impairment due to
increased placental resistance and poor renal perfusion.11 Cardiovascular changes occur early in pregnancy and progress with increasing gestational age,
potentially causing signicant impact on the development of the cardiovascular system.4 6
Original article
Selective fetoscopic laser coagulation (LC) of placental anastomoses ideally interrupts intertwin blood transfer and offers a
causal treatment.1 LC has been shown to signicantly improve
cardiac pathology in recipients.12 In contrast, donors have to
face an acute volume overload immediately after LC.13
However, even in successfully treated survivors, cardiovascular
impairment has been reported in the neonatal period, which
may persist throughout childhood.4 Despite successful intrauterine LC, sustained cardiomegaly and increased prevalence of
RVOTO has been found in a subset of recipient twins.810 14
Further important risk factors for cardiovascular diseases in
adulthood are unequal placental share, intrauterine growth
restriction and prematurity, all of which can coexist with
TTTS.1 4 1517 Therefore, follow-up studies of cardiovascular
function in TTTS are of great interest, but long-term cardiac
studies after LC are still lacking. We hypothesised that twins,
despite fetal LC, are at risk of long-term cardiac dysfunction. To
clarify this hypothesis, we studied the 10-year outcome in twin
survivors of severe TTTS treated by LC.
with standard German percentiles.19 Mean values of quantitative echocardiographic parameters were calculated from three
consecutive cardiac cycles and compared with normal values for
European children.22 23 Doppler velocities of the outow tracts,
great arteries and ventricular inow were compared with
age-related reference values.21 The myocardial performance
index, a sensitive and prognostic important marker for fetal
cardiac involvement in TTTS,7 was calculated to assess the combined systolic and diastolic function. On tissue Doppler
imaging, mean systolic and early and late diastolic myocardial
velocities were calculated at the basal interventricular septum,
the lateral mitral annulus and the tricuspid annulus (workstation
QLab 5.0).
3D left ventricular volumes, related to body size, were calculated from 3D full-volume datasets (QLab 5.0).25 26 Indices of
left ventricular lling and ejection were determined from 3D
volumetime curves.27 28
METHODS
Study population
Investigations
All fetuses underwent detailed echocardiography and Doppler
sonography at the time of LC. At least mild myocardial hypertrophy was identied in all recipients; 10 had moderate to
severe tricuspid regurgitation, 4 had hydrops and 1 had functional pulmonary atresia (table 2). Seventeen recipients had no
further cardiac abnormalities before fetal intervention.
Postnatally, a single examiner, blinded for donor and recipient
status, performed a complete echocardiographic examination
according to published standards using a Philips ultrasound
system (ie, 33, transducer X7-2; S8; S5; Andover, USA).1720
Data were analysed by two examiners, blinded to age, body
surface and recipient or donor status.
The mean of three consecutive measurements of the blood
pressure (BP), recorded with an oscillometric sphygmomanometer in a supine position after 20 min of rest, was compared
Statistical analyses
RESULTS
Structural heart disease
Structural heart disease was found in 6 of 62 survivors (9.7%;
table 3). Pulmonary stenosis (PS) was more common (5/62;
8.1%) than in the general population ( prevalence 0.066%, relative risk 134.4, 95% CI 42.1 to 428.8, p<0.0001).29 PS was
found both in recipient and donor twins, with two donors
requiring balloon dilatation within the rst months of life.
There was no correlation between the postnatal occurrence of
structural heart disease and abnormal prenatal cardiac ndings
before LC (table 2).
Blood pressure
Systolic and diastolic BP did not differ between twins, twins and
controls or discordant twin pairs.
Low birth weight (<3rd centile, n=9, and <10th centile,
n=27) was not associated with increased BP.
F381
Original article
Table 1 Perinatal and infant demographic profile in the three cohorts
GA at birth (weeks)
GA at LC (weeks)
LC-birth-period (days)
Weight at birth (g)
Height at birth (cm)
Weight at birth (z-score)
Height at birth (z-score)
Current age (years)
Current weight (kg)
Current height (cm)
Current BSA (m2)
Recipient (n=31)
Donor (n=31)
Control (n=15)
Intertwin
difference
p Value
RD
RC
DC
440315
2.42.4
1.270.93
0.890.86
<0.001
<0.001
<0.001
<0.001
2.573.29
1.482.85
0.050.08
<0.001
0.004
<0.001
<0.001
0.001
<0.001
0.001
ns
ns
ns
ns
<0.001
<0.001
<0.001
<0.001
ns
ns
ns
ns
Term
3333565
52.02.2
0.500.9
0.50.43
10.081.0 (range 8.5811.3)
34.396.71
141.97.7
1.1550.137
Values are meanSD; p Values by paired t test for intertwin analysis (RD, recipient to donor) and ANOVA for twin-control analysis (RC, recipient to control; DC, donor to control).
BSA, body surface area using the Haycock estimation; GA, gestational age; LC, laser coagulation; LC birth period, period between laser therapy and birth; ns, not significant.
DISCUSSION
To our knowledge, this is the rst childhood follow-up study of
cardiac function in twin pairs after intrauterine laser therapy of
Present
CHD
Prenatally
Not
present
CHD
p
Value
4
3
10
1*
1*
1*
27
28
21
3
3
3
0.511
0.408
0.510
13
1*
18
0.518
30
0.846
F382
Donor
PDA, coiled
Original article
Table 4 Haemodynamic variables in recipients, donors and controls
Variable (meanSD)
Recipient
HR (bpm)
7811
BP sys z-score
0.830.87
BP dia z-score
0.080.96
M-mode echocardiography
IVSd z-score
1.441.39
LVPWd z-score
0.150.82
RVIDd z-score
0.261.58
LVIDd z-score
0.080.89
LA z-score
0.151.43
Ao z-score
0.140.94
FS MM (%)
37.76.3
Doppler echocardiography
Ao Vmax (m/s)
1.110.23
PA Vmax (m/s)
1.140.53
Mitral E wave (m/s)
0.960.14
Mitral A wave (m/s)
0.560.11
Mitral E/A ratio
1.80.4
Tricusp E wave (m/s)
0.740.12
Tricusp A wave (m/s)
0.510.11
Tricusp A wave no PS
0.490.09
Tricusp E/A-ratio
1.50.23
MPI LV
0.350.20
MPI RV
0.230.18
2D echocardiography
0.750.15
EDV/(aBSA1.38)
0.780.19
ESV/(bBSA1.37)
EF 2D (%)
60.06.2
3D echocardiography
0.720.12
EDV/(aBSA1.38)
0.820.14
ESV/(bBSA1.37)
EF 3D (%)
56.85.1
Volume-time-relation on 3D echocardiography
1/3 FR/EDV
2.30.72
PFR/EDV
3.00.5
TPFR (ms)
84.510.4
Tissue Doppler imaging
8.11.2
Mitral sept E0 (cm/s)
8.21.6
Mitral sept E0 no PS
Mitral sept A0 (cm/s)
3.01.1
122.67
Mitral sept E/E0
9.32.2
Mitral lat E0 (cm/s)
3.10.8
Mitral lat A0 (cm/s)
11.23.1
Mitral lat E/E0
7.81.8
Tricusp E0 (cm/s)
3.93.2
Tricusp A0 (cm/s)
9.82.7
Tricuspid E/E0
Intertwin difference
p Value
Donor
Control
Recipientdonor
RD
RC
DC
31
31
31
7811
0.600.55
0.040.51
31
31
31
768
0.420.51
0.010.43
15
15
15
0.2811.6
0.240.96
0.120.80
31
31
31
ns
ns
ns
ns
ns
ns
ns
ns
ns
30
30
29
30
30
30
30
1.181.46
0.070.81
0.261.40
0.031.02
0.490.85
0.040.99
37.37.4
31
31
31
31
29
29
31
1.580.98
0.130.71
0.661.87
0.051.10
0.121.44
0.180.59
38.17.1
15
15
15
15
14
14
15
0.272.08
0.201.10
0.492.24
0.081.22
0.391.50
0.010.85
0.489.10
30
30
29
30
29
29
30
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
30
31
31
31
31
31
31
25
31
31
30
1.060.17
1.040.27
1.010.16
0.570.13
1.850.44
0.720.15
0.450.10
0.440.11
1.640.36
0.290.13
0.240.21
31
31
31
31
31
31
31
25
31
31
31
1.010.17
0.900.10
0.940.12
0.490.11
1.980.46
0.650.15
0.440.08
0.440.08
1.520.41
0.360.15
0.250.13
15
15
15
15
15
15
15
15
15
15
15
0.060.23
0.100.54
0.050.19
0.010.14
0.080.39
0.020.16
0.060.13
0.431.34
0.150.45
0.060.19
0.020.27
30
31
31
31
31
31
31
25
31
31
30
ns
ns
ns
ns
ns
ns
0.009
0.119
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
29
29
29
0.760.16
0.780.22
60.76.8
29
29
29
0.680.12
0.700.13
60.05.4
15
15
15
0.010.18
0.020.28
1.266.9
27
27
27
ns
ns
ns
ns
ns
ns
ns
ns
ns
30
30
30
0.740.11
0.890.19
54.96.3
30
30
30
0.790.10
0.950.16
54.45.5
15
15
15
0.030.11
0.070.17
1.67.8
29
29
29
ns
ns
ns
ns
ns
ns
ns
ns
ns
30
30
30
2.40.5
3.00.5
85.714.2
30
30
30
2.30.7
3.10.5
84.311.91
15
15
15
0.10.8
0.10.7
0.616.6
29
29
29
ns
ns
ns
ns
ns
ns
ns
ns
ns
09
09
09
09
09
09
09
06
06
06
0.71.5
0.61.7
0.21.5
0.73.7
0.051.8
0.70.6
0.43.3
0.12.5
0.14.1
0.54.0
22
16
21
22
10
09
10
05
04
05
0.044
0.202
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
ns
24
16
23
24
12
12
12
06
05
06
8.91.2
8.81.3
2.710.9
11.32.3
8.92.2
2.40.4
11.52.9
8.71.3
3.81.4
8.51.9
27
16
26
27
18
17
18
12
12
12
7.91.5
7.91.5
2.71.0
121.8
8.52.2
2.40.9
11.83.9
9.13.9
3.72.0
7.13.7
severe TTTS. Ten years after LC, surviving twins without structural
heart disease had normal heart dimensions and normal systolic and
diastolic function. Despite unequal loading conditions and cardiac
F383
Original article
Figure 1 Peak velocities during late diastolic lling of the right ventricle (TV-A Vmax) in the complete cohort including twins with pulmonary
stenosis and atrial septal defect (to the left) and in twin pairs without right ventricular heart disease (to the right). Lines connect the results within
each pair of twins. If twin pairs with right ventricular heart disease were excluded, there was no signicant intertwin difference.
Limitations
As this study only investigated surviving twin pairs after LC, but
no singleton survivors after intrauterine death of the co-twin,
no valid conclusion can be made for the complete collective of
all survivors with respect to cardiac and vascular risks. Because
our study design used an intertwin comparison, we may have
investigated a subgroup with better outcome.
Original article
It would be of particular interest to perform a comparative
investigation on twin pairs with severe TTTS, who have
received intrauterine amnioreduction. But as LC has become the
rst-line treatment for TTTS, we do not have access to an agematched collective treated by amnioreduction. In addition, a
control group of age-matched unaffected monochorionic twins
was not available.
Certainly pregnancies were followed up at the local referring
hospitals, but postinterventional and detailed neonatal data are
not completely available.
Our study placed particular emphasis on the investigation of left
ventricular function because according to the Barker hypothesis,
arterial hypertension, and therefore, left ventricular dysfunction,
was expected. Future studies should include the most sensitive
parameters of both right and left ventricular function in all twin
pairs. Investigation of global and segmental myocardial tissue
deformation of both ventricles in long-term survivors of TTTS
would be of interest.11 Given the high number of negative results
and only subtle differences between recipient and donor in this
cohort, further studies with a larger sample sizeespecially with
regard to diastolic dysfunctionare necessary.
Although this study is the largest long-term follow-up study
of survivors after LC of TTTS, we were not able to examine all
twin survivors eligible.
CONCLUSION
Ten years after LC of severe TTTS, the majority of surviving
donors and recipient twins had normal cardiac ndings. Despite
unequal loading conditions and cardiac impairment in utero,
there were no differences between donors and recipients longterm cardiac performance. Discordant birth weight or birth
weight <3rd centile had no inuence on BP or cardiac function.
This illustrates that cardiac dysfunction present in survivors after
serial amnioreduction is not found after LC and indicates that
cardiac dysfunction regresses once successful LC has removed
the underlying cause. However, long-term follow-up into adulthood is necessary to verify these ndings.
In addition, the increased prevalence of congenital heart
disease both in recipients and donors justies the need for prenatal and postnatal cardiac surveillance.
Contributors All authors contributed broadly to the work presented in this paper.
UH and JBr designed the study. PG, PB and KH were responsible for patient
acquisition. UH ran the clinical examinations, measured blood pressure and
performed echocardiography. Echocardiographic data analysis was performed by JB
and JBr Statistical evaluation was realised by UH and JB Anthropometric
measurement was conducted by PG Prenatal intervention was carried out by KH The
manuscript was supervised by JBr, KH and PB and edited by UH and JB.
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REFERENCES
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4
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F385
doi: 10.1136/archdischild-2013-305034
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Notes