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MD 2Y1-2
INTRODUCTION
ENZYME
-organic compound that acts as a CATALYST for a
biochemical reaction
-help reaction to occur more rapidly
-undergo denaturation
-lowers activation energy for the formation of
product
SUBSTRATE
-reactant in an enzyme-catalyzed reaction
ENZYME-SUBSTRATE
COMPLEX
LOCK-AND-KEY
MODEL
INDUCED-FIT MODEL
ENZYME SPECIFICITY
1. Absolute Specificity
-Catalyze reactions for only ONE substrate.
2. Stereochemical Specificity
-Catalyze reactions of L-amino acid
STEREOISOMERS.
3. Group Specificity
-Catalyze reactions of structurally similar
compounds with the same FUNCTIONAL GROUPS.
ENZYME ACTIVITY
4 FACTORS AFFECTING
ENZYME ACTIVITY
TEMPERATURE
-As the temperature of an enzymatically
catalyzed reaction increases, so does the rate of the
reaction
4 FACTORS AFFECTING
ENZYME ACTIVITY
pH
-Small changes in pH in the active site can
result to denaturation or loss of catalytic activity.
-Optimum pH: 7.0 - 7.5pH
4 FACTORS AFFECTING
ENZYME ACTIVITY
SUBSTRATE CONCENTRATION
-When substrate concentration increases, the
reaction rate increases up to a certain concentration
and then remains constant.
4 FACTORS AFFECTING
ENZYME ACTIVITY
ENZYME CONCENTRATION
-When enzyme concentration increases, the
reaction rate increases because more substrate can be
accommodated.
enzyme concentration =
reaction rate
MAJOR CLASSES
1. Oxidoreductase
2. Transferase
3. Hydrolase
4. Lyase
5. Isomerase
6. Ligase
OXIDOREDUCTASE
OXIDOREDUCTASE
SUBCLASS
REACTION
CATALYZED
EXAMPLE
Oxidases
Oxidation of a
substrate
Glucose Oxidase,
Galactose
Oxidase
Reductases
Reduction of a
substrate
HMG-CoA
reductase
Dehydrogenases
Introduction of a
double bond by
removal of 2 H
atoms from
substrate and
accepted by a
coenzyme
Lactate DH,
Pyruvate DH
TRANSFERASE
TRANSFERASE
SUBCLASS
REACTION
CATALYZED
EXAMPLE
Transaminases
Transfer of an amino
group between
substrates
Aspartate
Transaminase
Kinases
Transfer of a
phosphate group
between substrates
Hexokinase
HYDROLASE
LYASE
ISOMERASE
NOMENCLATURE
ISOMERASES
LIGASES
NOMENCLATURE
CLASSIFICATION
LIGASES
Acetate + CoA +ATP
CoA+AMP+PP
Succinate + CoA + NTP
NDP+ Pi
Pyruvate + CO2 + ATP
ADP + Pi
Acetyl CoA
Synthetase
Succinyl CoA
Acetyl
Succinyl CoA +
Synthetase
Pyruvate
Oxaloacetate +
Carboxylase
(Activated fatty acid) + AMP + PP
Fatty Acyl CoA
+ CoA + ATP
Synthetase
Fatty acid