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Applications of AAS
and AES
Structure
11.1
Introduction
Objectives
11.2
11.3
11.4
Sample Preparation
Applications of AAS
Biological Samples
Environmental Samples
Industrial Samples
11.5
Applications of AES
Biological Samples
Geological Samples
Environmental Samples
Industrial Samples
11.6
11.7
11.8
Summary
Terminal Questions
Answers
11.1 INTRODUCTION
In the preceding Units 9 and 10, you have studied about basic principles and
instrumental aspects of atomic absorption spectrophotometry (AAS) and atomic
emission spectrometry (AES) respectively. You would recall that atomic absorption
spectrophotometry concerns the absorption of radiation by the atomised analyte
element in the ground state; the atomisation being achieved by the thermal energy of
the flame or electrothermally in an electrical furnace. The wavelength(s) of the
radiation absorbed and the extent of the absorption form the basis of the qualitative
and quantitative determinations respectively. On the other hand atomic emission
spectrometry concerns the emission of radiation by the suitably excited atomic
vapours of the analyte; the atomisation as well as the excitation being achieved by any
of the numerous available energy sources such as flame arcs, sparks, or plasmas. Here,
the emitted radiation and its intensity form the basis for the qualitative and quantitative
applications of the technique. You have also learnt about flame emission
spectrophotometry (FES), another atomic emission technique, commonly called as
flame photometry in Unit 7. You would recall that it is a simple, rapid and inexpensive
method for routine analysis of alkali and alkaline earth metals like, sodium, potassium,
lithium, calcium and barium in environmental, clinical and biological samples
especially in biological fluids and tissues.
In this unit, we take up some of the important applications of atomic absorption
spectrophotometry and atomic emission spectrometry. We will begin the unit with
recalling the salient features of the two techniques and then take up the applications of
AAS and AES. In the next block you would learn about some miscellaneous
spectroscopic methods.
Objectives
After studying this unit, you will be able to:
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Atomic Spectroscopic
Methods-II
11.2
You know that in atomic spectroscopy, the element present in a sample is converted to
gaseous atoms or elementary ions in a process called atomisation which may be
brought about by any of the available methods. The absorption of the radiation by the
vapourised atoms in the ground state, or emission or fluorescence emission of suitably
excited state forms the basis of different types of atomic spectroscopies. Collectively,
the atomic spectroscopic methods can be used for the qualitative and quantitative
determination of about 70 elements in a wide variety of samples of clinical, biological,
and environmental origin. You have learnt in details about AAS and AES in Units 7,9
and 10. Let us recollect the salient features of the AAS and AES methods before
taking up their applications.
11.2.1
58
Compounds of the alkali metals, some of the heavy metals such as lead or
cadmium and transition metals like manganese or nickel are all atomised with
good efficiency by flame However, a number of refractory elements like V, Zr,
Mo and B do not perform well with a flame source.
GFAAS requires a very small sample size and does not require any sample
preparation; even solid samples can be analysed without dissolution.
Accuracy in AAS method is generally of the order of 0.5 5%; the precision
being 0.3 1% at absorbance larger than 0.1 or 0.2 for flame atomisation and
1 5% with electrothermal atomisation.
It is a robust technique that employs easy to use equipment and can achieve
good detection limits. As the turnaround time is small the cost of analysis per
sample is not much. However, lack of automation, and usage of flammable
gases are not desirable.
11.2.2
As the energy of the plasma source is quite high, it ensures the excitation of the
atoms of all the elements present in the analyte which emit EM radiation
characteristic of different elements. Thus, it is a multielement technique.
Argon gas is commonly employed as plasma gas due to its inertness, simple
emission spectrum, moderately low thermal conductivity, and good natural
abundance.
Two types of spectrometers are used for ICP-AES. These are sequential
spectrometers and simultaneous spectrometers depending on whether the
emitted radiation is measured sequentially or simultaneously.
In ICP-AES the spectral interference due to the line-rich spectra of the hot
plasma source can be minimised by using high resolution spectrometers or using
an alternative analyte line. The background effects require the use of offline
background correction techniques, or by moving to an unaffected analyte line.
The matrix effects are generally handled by using internal standard method.
11.2.3
Applications of AAS
and AES
As has already been emphasised, AAS and AES have become the mainstay of the
analytical techniques for major, minor and trace element analysis in geological,
biological, environmental and industrial samples. Both the techniques can be used for
the determination of more than sixty elements, many of which can be determined at
1 ppm level. As regards their applicability, these two techniques are complementary to
each other though several points are common amongst them.
It must be kept in mind that only metals and metalloids can be determined by usual
flame methods like FAAS. This is because resonance lines for nonmetals fall in
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Atomic Spectroscopic
Methods-II
vacuum UV region though some indirect methods have been developed for the same.
For example, chloride can be determined by precipitation with Ag+ and then either the
excess of Ag+ or the one which has already reacted is measured. Similarly phosphorus
(525.9 nm) and sulfur (383.7 nm) species exhibit sharp molecular band emission in the
Ar-H2 flame. Generally, AAS is considered as more sensitive technique at wavelengths
< 300 nm, whereas in visible region, AES is more advantageous. Some elements
exhibit maximum sensitivity using molecular band emissions.
As the source of radiation in AAS is a hollow cathode lamp which emits the
characteristic radiation of a given element, it is a unielemental technique. It is not
convenient to measure more than one element at a time by AAS as it is difficult to
incorporate more than a single source into the system. Each hollow cathode lamp
emits efficiently the spectrum of only one, two, or three elements at a time, measuring
additional elements requires substituting a new hollow cathode lamp. Though some
advances have been made in continuum source atomic absorption spectrophotometry
yet these arrangements are somewhat limited as sources extending into the ultraviolet
region of the spectrum are not widely available.
The basic principle of graphite furnace atomic absorption spectrophotometry
(GFAAS) is essentially the same as flame atomic absorption spectrophotometry, the
only difference being that the atomisation is achieved in a small, electrically heated
graphite tube, or cuvette, which is heated to a temperature up to 3000C to generate
the cloud of atoms. The higher atom density and longer residence time in the
electrothermal tube improve the detection limits by a factor of up to three orders of
magnitude as compared to flame AAS and we can go down to the sub-ppb range.
However, the use of graphite cuvettes, do not sort out the issue of determining
refractory elements.
It is essential that the AAS instrument should always be calibrated by preparing at
least four standard solutions over the concentration range of interest and measuring the
absorbance under the same experimental conditions. The correction, if necessary,
should be applied to the calibration plot. Sometimes, the method of standard addition
is used to compensate for chemical and other interferences.
In contrast to atomic absorption spectrophotometry, atomic emission spectrometry is
inherently a multielement method. Especially the high temperature of plasma ensures
effective atomisation and lead to intense atomic emission. The emission occurs from
all elements at the same time and is isotropic. The simultaneous multielement
determinations can be made simply by using a multichannel detection system.
Multichannel devices using two dimensional spectral dispersion along with two
dimensional arrays of detector elements offer extremely good sensitivity and low
noise.
More so at the operating high temperatures of ICP torch, even the most refractory
elements are atomised with high efficiency. As a result, detection limits for these
refractory elements can be of the orders of magnitude lower with ICP than with FAAS
techniques. These may be at the 1-10 parts per billion level. We can safely generalise
the order of detection limits of different techniques as GFAAS (sub-ppb) > ICP-AES
(1-10 ppb) > FAAS (sub-ppm).
Further, the dynamic range of the various techniques is also important, as it directly
affects the amount of dilution required in preparing solutions for analysis. If the
dynamic linear range is quite wide, we may use fewer standards. The dynamic ranges
of FAAS and GFAAS are of the order of only 102-103 only whereas the same for ICPAES the dynamic range spreads upto 106. This makes it a suitable technique that is
capable of measuring from trace to percent levels. A comparative account of the
characteristics of AAS and ICP-AES are briefly summarised in Table 11.1.
60
11.3
Applications of AAS
and AES
SAMPLE PREPARATION
All samples for determination by AAS or AES must be in solution form except for
spark source AES where solids especially metals and alloys with smooth surface can
be analysed directly. The detailed procedures for sample preparations have been
discussed in Section 9.7 and subsection 10.4.2 respectively for AAS and AES. You
would recall that in principle, the sample in solid, liquid or in the gas phase can be
analysed by flame AAS but in practice the sample is taken in the solution form. The
solution of the solids is generally prepared by wet dissolution method using a suitable
acid. The presence of organic solvents of low molar mass e.g. alcohols, ethers, ketones
and esters are found to enhance absorption peaks and hence increase sensitivity. A
microwave digestion system (MDS) offers more rapid and efficient decomposition of
complex matrices of geological and biological samples. It greatly reduces the operator
time to prepare samples for analysis. More so, it can be easily automated also.
On the other hand in ICP-AES, the solution preparation depends on the nature of the
sample and the concentration of elements to be determined. The solution for ICP
analysis can be prepared either by wet acid method or by direct attack method and
suitable precautions are taken as per the requirements of the plasma source.
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Methods-II
It must be noted that the possible contamination during dissolution and at the
workplace is the most important source of error in the analysis of trace elements and
must be avoided. Contamination may come from the air, from the skin of the subject/
sample collector, additives and reagents used in the analysis, as well as parts of
instrumentation including glass or plastic wares. Biological materials of human and
plant origin must be handled with extreme care because of sample inhomogeneity
especially for trace element analysis. Body fluids such as blood, viscera, urine, etc.
additionally need stabilization and homogenization so as to avoid occurrence of any
changes in their composition, prior to actual analysis. It is also advisable to keep the
total number of transfers to a minimum, and to use accessories made of non-wettable
and inert materials.
SAQ 1
What precautions should be observed while preparing samples for AAS and AES?
..
..
...
...
11.4
APPLICATIONS OF AAS
11.4.1
Biological Samples
A wide range of the samples of biological origin are subjected to analytical procedures
for the determination of the elements present in them. These may include plant leaves,
fruits, vegetables, blood, urine, muscle tissue, hair, etc. The major difficulty in the
analysis of these materials is their complex nature. More so, these samples cannot be
analysed directly but require dry ashing followed by wet digestion with oxidising
acids such as HNO3 and HClO4 . In case of blood analysis, plasma or serum is
generally preferred because of the presence of significant amounts of clinically
significant elements in them.
i)
62
Similarly the effect of organic solvent such as ethanol may also be studied. The effects
of interferants is borne by using 5 ppm each of phosphate, sodium and aluminium
solutions
Applications of AAS
and AES
Determination of cadmium
Cadmium is one of the most important toxic elements from the environmental point of
view. It occurs in nature mainly due to volcanic activity. It is used in plating of metals,
as stabiliser in polyvinyl chloride, pigments, Ni-Cd batteries and alloying. It is the
prime cause of itai-itai disease first observed in Japan. Cadmium along with lead has
been the most studied element with regard to human toxicology as it has no role in
human or plant nutrition. It is highly toxic even in trace amounts to the human body.
Total intake of cadmium in Germany, USA and most European countries is in the
range, 10-30 g/day whereas in contaminated areas of Japan, its intake is as high as
400 g/day. It is most likely to be ingested by tobacco smoking especially cigarettes.
Absorption of cadmium is higher in females than in males though its transport in the
intestinal tract is influenced by the presence of various food components such as
proteins and amino acids.
Cadmium in blood may be used as a biological monitoring measure for recent
occupational/environmental exposure. In addition, cadmium in urine may also be used
as a measure of biological monitoring for body burden where it reflects the total
accumulation of cadmium in the body. Typically it occurs at 1g/L in the blood of
healthy and nonexposed nonsmokers in various countries. Considering the
requirements of detection limit and contamination free sample handling, graphite
furnace atomic absorption spectrophotometry is the method of choice where the
detection limit is 0.04 g/L.
A typical determination of cadmium in blood involves de-proteination with nitric acid
followed by direct determination by GFAAS using source with 228.8 nm output. The
blood sample is collected in plastic collection tubes using vinyl gloves free of talc and
is stored at a temperature of 20 oC to 4 oC. All the laboratory ware is to be soaked in
diluted nitric acid and biodistilled quality water is used for dilution work. The
determination is preceded by the obtaining calibration curves using matrix adapted
calibration solutions. In simple words it means that the calibration solution contains all
the known components of the analyte sample. A multiple standard calibration is
preferred. Similarly, Cd could also be determined in urine, hair and other body tissues.
iii)
Determination of lead
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Methods-II
Zinc is an essential nutrient in plants and remains distributed in all parts of the plant.
About 1g dried plant leaves are grounded with pestle and mortar and dry ashed in
silica crucible at 500C. The ash is then dissolved in acid and final solution is prepared
to 0.1 M HCl. The solution is then directly aspirated into an air-acetylene flame of
AAS. A blank is also prepared in exactly similar manner.
11.4.2
Environmental Samples
Air, soil and water are three components of environment where determination of toxic
contaminant is of extreme importance. Analysis of particulate matter in air from
industrial establishments is the most representative study of environmental samples by
atomic spectrometry.
i)
In the analysis of airborne particulate matter, the choice of sample collection location
and collection procedure is very important. For example samples may be collected
from surrounding areas of a factory emitting harmful gases affecting workers health
adversely or a residential colony located near industrial establishment where toxic
pollutants may travel and thus affect general public. A measured volume of air is
collected on a cellulose acetate membrane filter using air sampler. Weighed amount of
particulate matter is scratched out of the filter paper or the filter paper itself may be
dry ashed in a low temperature furnace so as to avoid loss of volatile elements. The
particulate matter or ash is then dissolved using acid digestion method and heating on
hot plate. The final solution is prepared in dilute HCl and making up final volume to a
fixed volume. Appropriate hollow cathode lamps are selected depending on the
elements to be determined and respective standard solutions are prepared. Calibration
plots should be drawn for each element to be determined and the test solution is
aspirated. Thus concentration of desired elements in air dust particulate matter may be
determined. Results are usually reported in terms of g/m3 of air.
ii)
Mercury in air/water
Metallic mercury is important as it forms amalgam with other metals. Its alloy with
silver was earlier used by dentists for dental filling though it is no longer so because of
toxicological effects known since many years. Mercury in air is collected by bubbling
air through an acidic KMnO4 solution where volatile elemental mercury is trapped by
oxidising it to Hg2+. The excess permanganate is reduced with hydroxylamine, and the
collected mercury (or mercury in a water sample) is then reduced to the element by
SnCl2 according to following equations.
5Hg0 + 2MnO 4 + 16H+
prepared in the same manner, omitting the addition of mercury. The measured
absorbance is corrected for the blanks and the amount of mercury is determined from
the calibration curve.
Applications of AAS
and AES
Similarly, the water sample from tap, river, or other sources can be analysed. Tap
water should contain around 1 ppb or less mercury. In such determinations the water
samples and the standards should be run in a similar manner. As in the case of air
samples, the correction should be made for the reagent blank as its magnitude will
generally govern the detection limit of the procedure. Extreme care must be taken to
minimise reagent and glassware contamination. From the calibration graph the
quantity and concentration of mercury in the sample is determined.
iii)
11.4.3
Industrial Samples
Quality control of finished products of steel industry and other products such as alloys
requires accurate analysis. For such an analysis an alloy or steel is be dissolved in acid
(HCl, HNO3, HClO4 or a mixture of these) and a solution is prepared for analysis by
AAS. Care must be taken to eliminate excess of acid. A typical example is described
in following lines.
i)
Weighed amount of sample is dissolved in aqua regia and finally in dil HCl. Final
volume is made up to fixed volume in a volumetric flask by adding doubly distilled
water. Molybdenum can be determined in acetylene-air or acetylene-N2O flame
selecting a wavelength of 313.26 nm. Let us take up an example.
Example
0.32 g stainless steel sample was dissolved in nitric acid and the resulting solution was
made to 100 cm3 with water. Five standards and the sample solution were aspirated
into flame for the determination of nickel. The following observations were made.
Concentration of Nickel (ppm)
Absorbance
0.126
0.250
0.374
0.500
10
0.626
Sample
0.226
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Atomic Spectroscopic
Methods-II
Solution
Let us prepare the calibration plot by taking the concentration on X-axis and
absorbance on Y-axis, as shown below
According to the calibration plot the sample concentration is found to be 3.612 ppm. It
corresponds to 1.12%.
ii)
SAQ 2
Enlist some important applications of AAS in the area of environmental analysis.
..
..
...
...
11.5
APPLICATIONS OF AES
11.5.1
Applications of AAS
and AES
Biological Samples
As you have learnt above, a wide range of the samples of biological origin are
subjected to analytical procedures for the determination of the elements present in
them. Let us take up the determination of sodium in serum as a representative example
of the application of AES in biological samples.
Determination of sodium in serum
Determination of sodium in water or serum is carried out by following the
characteristic emission at 589 nm. A calibration plot is prepared between emission
intensity and concentration of the standard solutions. The concentration of the sample
solution is then determined from the calibration plot. In some of the determinations a
known amount of an internal standard like lithium is added to the standard solutions as
well as the sample solution. The calibration curve is drawn between the emission
intensity ratios of the characteristic emissions of sodium to lithium versus the
concentration of the standard solutions of sodium.
11.5.2
Geological Samples
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Methods-II
11.5.3
Element
Detection limit
Element
Detection limit
Ag
0.004
Mo
0.0001
Al
0.00008
Na
0.00002
As
0.002
Ni
0.0001
Au
0.04
0.015
0.0001
Pb
0.001
Ba
0.00001
Pd
0.0008
Be
0.000003
Pt
0.08
Ca
0.0000001
Rh
0.003
Cd
0.0002
Sc
0.003
Ce
0.0004
Se
0.03
Co
0.003
Si
0.01
Cr
0.0008
Sn
0.003
Cu
0.0006
Sr
0.00003
Fe
0.00009
Th
0.003
Ga
0.0002
Ti
0.00003
Hf
0.01
Tl
0.2
Hg
0.01
0.03
In
0.03
0.00006
La
0.001
0.0007
Mg
0.000003
Zn
0.00001
Mn
0.00002
Zr
0.00006
Environmental Samples
68
11.5.4
Industrial Samples
Applications of AAS
and AES
SAQ 3
Enlist any five elements present in rock samples that are expressed in terms of their
oxide equivalents.
..
..
...
...
SAQ 4
What do you understand by wear metal analysis? What is its significance?
..
..
...
...
...
11.6
SUMMARY
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Atomic Spectroscopic
Methods-II
11.7
TERMINAL QUESTIONS
1.
2.
3.
Plasma,
b)
Spectral interference
4.
5.
6.
a)
b)
c)
Absorbance
0.201
10.0
0.292
20.0
0.378
30.0
0.467
40.0
0.554
11.8
ANSWERS
70
all possible contamination coming from the air, from the skin of the
sample collector, additives and reagents used in the analysis, as well as
parts of instrumentation including glass or plastic wares should be
avoided.
2.
body fluids such as blood, viscera, urine, etc should be stabilised and
homogenised so as to avoid occurrence of any changes in their
composition, prior to actual analysis.
Applications of AAS
and AES
3.
4.
The wear metal analysis refers to the determination of metals in used lubricating
oils from the aircraft, truck, locomotive and other engines. This provides an
assessment of the mechanical wear and tear of the engine.
Terminal Questions
1.
In case of ICP-AES all the elements get excited at the same time in the plasma
torch. The radiation emitted by them can be measured sequentially or
simultaneously. Hence it is easier to determine several elements
simultaneously. However, in case of AAS a line source hollow cathode lamp is
used as the radiation source. As only one analyte element is able to absorb the
radiation emitted by the cathode lamp we can measure only one element at a
time. For multielemental determination by AAS we need to use a cathode lamp
for each element thus making it a difficult task.
2.
Basic difference between atomic emission (AES) and atomic absorption (AAS)
spectrometry is the source of radiation and the measured parameter. In AES, the
source of radiation is sample itself where the energy for excitation of analyte
atoms is supplied by plasma, a flame, an oven or an electric arc or spark. The
signal is the measured intensity of the source at the wavelength of interest. On
the other hand in case of AAS, the source of radiation is a line source such as
hollow cathode lamp. The signal is in terms of absorbance calculated from the
radiant power of the source and the resulting power after the radiation has
passed through the atomised sample.
3.
a)
b)
ions
4.
5.
a)
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Atomic Spectroscopic
Methods-II
6.
72
b)
c)
2.
3.
4.
5.
6.
Analytical Chemistry by G. D. Christian, 6th Edn, John Wiley & Sons Inc,
Singapore (2003), Ch. 15.
7.
8.
9.
10.
11.
12.
13.
Applications of AAS
and AES
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Atomic Spectroscopic
Methods-II
INDEX
Absorbance 7, 8, 9, 14, 59, 63, 65
Acid digestion method 40, 64
Advantages and disadvantages of GFAAS 16
Agricultural science 51
Analytical methodology in ICP-AES 47
Qualitative analysis using ICP-AES 48
Characteristic line groupings 48
Line coincidences 48
Persistent or RU 48
Spectral line tables 48
Quantitative analysis 48
Environmental samples 64
Analysis of airborne particulate matter 64
Mercury in air/water 64
Trace element contamination in soil 65
Industrial samples 65
Determination of molybdenum in steel 65
Tin in canned fruit juice 66
Applications of AES 66
Biological samples 67
Determination of sodium in serum 67
Geological samples 67
Environmental samples 68
Trace elements in airborne particulate matter 68
Industrial samples 69
Determination of metals in lubricating oil 69
Applications of ICP-AES 51
Agricultural science 51
Environmental science 51
Forensic sciences 51
Geological sciences 51
Health sciences 51
Industry 51
Metallurgy 51
74
Chemical interferences 20
Chemical interferences 50
Choice of argon as plasma gas 38
CID based instruments 47
Comparison between AAS and AES 59
Concentration dependence of absorption 7
Continuum sources 10
Dc electrical source 34
Detectors 13, 39
Direct current plasma 37
Double beam atomic absorption spectrophotometer 18
Dry attack method 41
Echelle spectrometers 46
Electrodeless discharge lamps 11
Electrothermal atomisers 11, 14
Electrothermal vaporisation 42
Environmental samples 64, 66, 68
Environmental science 51
Filament 14
Flame atomiser 11
Forensic sciences 51
Frit nebuliser 42
Fuel-oxidant ratio 12
Furnace atomic absorption spectrophotometry 14
Geological samples 67
Geological sciences 51
Graphite furnace 15, 58, 60
Graphite furnace atomic absorption spectrophotometry 14
Applications of AAS
and AES
Filament 14
Furnace atomic absorption spectrophotometry 14
Graphite tube 14
Handling background absorption in GFAAS 16
LVov furnace 14
Graphite tube 14
Handling background absorption in GFAAS 16
Health sciences 51
Hollow cathode lamp 11
Hydride generation 42
Hydride generation technique 24
Industrial samples 59, 65, 69
Industry 51
Instrumentation for ICP-AES 39
Detector 39
Monochromator 39
Nebuliser 39
Plasma source 39
Processing and readout device 39
Sample Introduction 40
Dry attack method 41
Electrothermal vaporisation 42
Frit nebuliser 42
Hydride generation 42
Nebulisation 41
Nebuliser 42
Nebulisers for ICP-AES 42
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Atomic Spectroscopic
Methods-II
Sample preparation 41
Acid digestion method 41
Ultrasonic 42
Flame atomiser 11
Fuel-oxidant ratio 12
Detectors 13
Monochromators 13
Radiation sources 10
Continuum sources 10
Electrodeless discharge lamps 11
Hollow cathode lamp 11
Line sources 10
Readout devices 14
Interferences in ICP-AES 50
Chemical interferences 50
Physical interferences 50
Spectral interferences 50
76
Applications of AAS
and AES
Quantitative methodology 7
Calibration plot method 8
Internal standard method 8
Standard addition method 9
Scrubbing 21
Use of organic solvents 22
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Atomic Spectroscopic
Methods-II
Simultaneous spectrometers 45
Polychromators 45
Echelle spectrometers 46
Ultrasonic 42
Ultrasonic nebulisation 24
Use of organic solvents 22
Work coil 36
78