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Thermo Scientific
FastAP Thermosensitive
Alkaline Phosphatase
#__
Lot: __
Expiry Date: _
Concentration:
Supplied with:
1 u/l
__ ml of 10X FastAP Buffer
Store at -20C
In total _ vials.
BSA included
www.thermoscientific.com/onebio
Description
Thermo Scientific FastAP Thermosensitive Alkaline
Phosphatase catalyzes the release of 5'- and 3'phosphate groups from DNA, RNA and nucleotides. This
enzyme also removes phosphate groups from proteins.
CERTIFICATE OF ANALYSIS
Endodeoxyribonuclease Assay
No conversion of covalently closed circular DNA to nicked
DNA was detected after incubation of 10 units of FastAP
Thermosensitive Alkaline Phosphatase with 1 g of
pUC19 DNA for 4 hours at 37C.
Ribonuclease Assay
No contaminating RNase activity was detected after
incubation of 10 units of FastAP Thermosensitive Alkaline
3
Phosphatase with 1 g of [ H]-RNA for 4 hours at 37C.
Labeled Oligonucleotide (LO) Assay
No degradation of single-stranded and double-stranded
oligonucleotide was observed after incubation with 10 units
of FastAP Thermosensitive Alkaline Phosphatase
for 4 hours at 37C and subsequent phosphorylation using
32
[- P]-ATP and T4 Polynucleotide Kinase.
Quality authorized by:
Jurgita Zilinskiene
Plasmid DNA
10X Thermo Scientific FastDigest Buffer
FastDigest Restriction Enzyme
FastAP Thermosensitive Alkaline Phosphatase
Water, nuclease-free (#R0581)
Total volume
1 g
2 l
1 l
1 l
to 20 l
20 l
1 g
(~1 pmol termini)
2 l
1 l (1 u)
to 20 l
20 l
Reaction mixture:
1X FastAP reaction buffer, 0.1-0.2 mg/ml of
phosphoprotein, 10 u of FastAP Thermosensitive Alkaline
Phosphatase. Incubate at 37C for 1 h.
Note
The reaction can be stopped by addition of a final
concentration of 50 mM EDTA (#R1021) or by addition of
a final concentration of 10 mM sodium orthovanadate
(Na3VO4).
The optimal incubation time and the enzyme
concentration must be determined experimentally for
each substrate.
The purchase of this product allows the purchaser to use it for preparing amplified
DNA fragments under a license from GE Healthcare of U.S. Patent Nos. 5,741,676
and 5,756,285 and other foreign patents.