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TABLE OF CONTENTS

1. Objective

2. Introduction

3. Materials and Methodology

3,4

4. Results and Discussion

5. Conclusion

6. References

EXPERIMENT 6: INVESTIGATION OF OXYGEN SOLUBILITY IN WATER


OBJECTIVE
-To illustrate that oxygen dissolves to varying degrees under different conditions.
.
INTRODUCTION
Oxygen is an essential element for all aquatic organisms that breathe. Therefore, there
is a direct relationship between the oxygen concentrations and exchanges occurring in a water
body, and the physiological status of aquatic organisms. Without oxygen at the bottom of the
water body, anaerobic bacteria (those that live without oxygen) produce acids. These acids
not only increase acidity, but also cause a massive release of phosphorus and nitrogen, two
major fertilizers, from the organic sediment and into the water column. These same anaerobic
bacteria put toxic gases in the water including hydrogen sulfide (that rotten egg smell),
ammonia, carbon dioxide and methane. These gases are all toxic to fish, beneficial bacteria
and insects.
Fish and aquatic animals cannot split oxygen from water (H2O) or other oxygencontaining compounds. Only green plants and some bacteria can do that through
photosynthesis and similar processes. Virtually all the oxygen we breathe is manufactured by
green plants. A total of three-fourths of the earths oxygen supply is produced by
phytoplankton in the oceans.
If water is too warm, there may not be enough oxygen in it. When there are too many
bacteria or aquatic animal in the area, they may overpopulate, using DO in great amounts.

METHODOLOGY
PROCEDURE
This experiment was about to evaluate the solubility of oxygen in 4 different sources
of water samples.
1.
2.
3.
4.

Tap water
Distilled water
Rain water
Drain water

- A sample of tap water was took and measured (DO)


- Then heated at 100C from room temperature.
- The heated temperature could not exceed 500C.
- The heated tap water was cool down to room temperature by continuous shaking and
stirring.
-Record the dissolved oxygen as soon as the temperature reached to the initial temperature
which is 280C.
-Finally, blow the tap water for about 2 minutes and record the dissolved oxygen.
- With the table of results obtained, we draw a graph of DO(dissolved oxygen) against
temperature.
RESULTS AND DISCUSSION
DO and pH
No.
1
2
3
4

Samples
Tap water
Distilled water
Drain
Rain

DO
7.55
7.84
7.71
7.18

pH
6.03
6.08
6.02
6.06

According to this graph, we can know that the pH of samples of water doesnt affect the D.O.
In compare the highest pH of distilled water and lowest pH of drain water.
DO of tap water with varies temperature
T0C
28
38
48
28(cool)
28(blow)

DO (mg/l)
7.10
6.28
5.82
7.21
7.48

A GRAPH OF DO (DISSOLVED OXYGEN) AGAINST TEMPERATURE

7.48
7.21
7.1

6.28

5.82

5
4
3
2
1
0
25

30

35

40

45

50

CONCLUSION
In summary, the first table show the measurement of dissolved oxygen in varies
sample, and the second table was the tap water with different temperatures. And we knew the
relation between the samples and pH is not affect each other.

REFERENCE:
1. http://www.clean-flo.com/restoration-basics/importance-of-oxygen/

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