Key:

Enterobacteriaceae

TD Triple- decarb.

E. coli
Klebsiella

Pantoea

Enterobac
ter Sp.
Citrobacte
r
Serratia
Salmonell
a
Shigella

Proteus
Morganell
a
Providenci
a
Yersinia

IMViC

PY
R

+,
+,=,=
=,=,+,
+

Ureas
e

Lysi
ne

V,
+,=,=
+,
+,=,=
+,+,=,
+
=,
+,=,=

TD

Res to:

Notes

B-lactamase,
cephalosporin

-Encapsulated
-ONPG +
- K. oxytoca Indole +
- Encapsulated
-Res is induced by 2nd & 3rd gen.
cephalosporins & Fluoroquinolones

+
+

=,=,+,
+
=,=,+,
+
V,+,=,
+
=,V,V,
+
=,
+,=,V
V,
+,=,=

Ornithi
ne

=
(Differs
from
Kleb)

Inducible Res to
B-lactamase

-Encapsulated

-Differed from E. coli by use of

citrate
-S. marcescens VP +

-S. typhi ODC =

-The most Biochemically

Nonreactive
- S. sonnei ONPG & ODC +
- S. dysenteriae only Cat =
-P. vulgaris indole +
- P. mirabilis indole =
-PMP in pink and green
suit (urease & PAD +)

+
+
+
=

-GS Safety pin

-Motile at 22C
Y. pestis Nonmotile
-Y. pseudo tuberculosis Bile-

esculin +
Y. enterocolitica V

All are:

1. Ox =

2. Catalase & Nitrate +

5. Non-spore

3. Motile
a.

6. Glucose fermenters

4.

Except for SKY

i. Shigella, Klebsiella & Yersinia

Enterobacteriaceae
TSI &
KIA

Mac

Hek

XLD

Bap

E. coli

A/Ag

LF

Hem

Klebsiell
a

A/Ag

LF

Pantoea

A/Ag

Y
R, Y
or C

Special
media

Key:
C
Clear/Colorless
Y Yellow
Notes
R Red
Green
- Most common cause of G
the
UTI
- Mac sorb 0157 clearBL Blue
-Abundant gas
Bc Black

(Mucoid
)

LF

Smooth, grey

-Abundant gas
- P. agglomerans Bap Lemon-Y

(Mucoid
)

Citrobac
ter

A/Ag,
HS

LF

(late)

(w or w/o
Bc)

Serratia

A/A

LF

Y or C

R with
Bc
C
C

-Resemble E. coli
-C. freundii most common isolate

(late)

Salmone
lla
Shigella

K/Ag,
HS
K/A

NLF
NLF

G/BL
with Bc
G

Proteus

K/A,
HS

NLF

C (w or

K/A

NLF

Morgane
lla

Facultative anaerobes

w/o HS)

R, C

-GM broth
-SS agar

Swarming

-S. marcescens Most common isolate

& the #1 cause of nosocomial infec in
nurseries
-Mac after 48hrs red pig. Or red
centers
-Abundant HS
-S. typhi Little to No HS or gas
-Mac LF after several days
-TSI W or w/o gas
-P. vulgaris swarms the most
-P. mirabilis more commonly isolated
-PMP Nosocomial infecs

Providen
cia
Yersinia

K/A

NLF

Y, C

K/Ag

NLF

Salmon
Pink

Y, C

-CIN

-TSI usually w/o gas

- Fried egg appearance as it ages
-CIN Target colonies
-Aeromonas also grows on CIN,
but is OX +

Media
Species

Serotype
STEC / EHEC

ETEC

E. coli
EPEC
EIEC
EAgEC

Serratia

Shiga
toxin
producing
E. toxigenic
E.
pathogenic
E. Invasive
E.
Aggregativ
e

Disease
.0157:H7
-childhood diarrhea in other
countries (bacterial cause)
-travelers diarrhea
- Daycare center / nursery
outbreaks
- Watery / bloody diarrhea

Notes
-The most IDd diarrhea Agent in North America
-Non-motile
-Mac sorbitol Clear
-Giardia is the parasite that causes it

- Diarrhea and kids in Chile, Mexico,

India, Japan
-Otitis externa

Salmonel S. enterica
la

Shigella

A.k.a

S. typhi
A S.
dysenteria
B S. flexner
C S.
bongori

Salmonellosis

Typhoid fever
-HUSS
-A & B Common cause of
Shigellosis (travelers diarrhea) in
developing countries

Foods
-from animal origin eggs, milk, poultry
-Non animal origin Contaminated HO,
animal
contact (reptiles), human contact
-P-P, fecal cont. food & water
-produce Shiga toxins
- doesnt ferment mannitol (the rest do)

D S. sonnei

Y. pestis

Yersinia

-In US D & B
Pneumonic plague
Key:
Black
death

Slant / Butt
Bubonic plague
A Yellow
(+)
Septicemic plague
K Red (=)

Y.
enterocolitica
Y. pseudo
tuberculosis

scarlet fever-like disease

Notes:
1. TSI
a.

Has Glucose, Sucrose & Lactose

i. Glucose is fermented
K/A

-Trans Rat Fleas

-Is a potential Bio-warfare agent
-Swollen Lymph nodes
-Septic shock
-Trans food, water, blood products (So is
listeria)
-iliosacral inflammation, mesenteric
lymphadenitis, septicemia, polyarthritis,
meningitis
-Prefers cool temps.

-GI infec, endotoxemia

ii. All 3 fermented

A/A

2. KIA
a. Has Lactose & Dextrose
i. Dextrose is fermented K/A
Lactose / Sucrose
ii. Both are fermented
A/A
iii. Neither ferment
K/K
3. TD test
a. Lysine, arginine, and ornithine
b. = means all 3 were =
Glucose