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DNA Sequence
(splited by genes)
RNA
Amino Acid
sequence
protein
phenotype
During
transcription
Gene 2
DNA
molecule
Gene 1
The gene
determines the
sequence of bases
along the length
of an mRNA
molecule
Gene 3
DNA strand
(template)
3
A
TRANSCRIPTION
mRNA
Codon
TRANSLATION
Protein
Trp
Amino acid
Phe
Gly
Ser
P : induk/orang tua/parental
berasal dari Bhs. Latin parens
F : keturunan
berasal dari Bhs. Latin filius
Fenotip
Genotip
Alel
Homozigot/Heterozygot
Diploid/Haploid
Tingkat Biokimiawi
Fenotip molekul protein, mis. fenotip globin
Aktifitas enzim, kadar gula darah
Tingkat Fisiologis
Hasil rekam elektrokardiografi, tekanan darah
Tingkat Histologis
Variasi bentuk sel
Tingkat Anatomis
Tinggi badan, warna kulit, warna rambut
Tingkat Psikologis
Tingkah laku, IQ
Hibrid
Persilangan Resiprok
EXPERIMENT
Bacteria of the S (smooth) strain of Streptococcus pneumoniae are pathogenic because they
have a capsule that protects them from an animals defense system. Bacteria of the R (rough) strain lack a capsule
and are nonpathogenic. Frederick Griffith injected mice with the two strains as shown below:
Living S
(control) cells
Living R
(control) cells
Heat-killed
(control) S cells
RESULTS
Mouse dies
Mouse healthy
Mouse healthy
Mouse dies
Living S cells
are found in
blood sample.
CONCLUSION
Griffith concluded that the living R bacteria had been transformed into pathogenic S bacteria by an
unknown, heritable substance from the dead S cells.
The Hershey-Chase
experiment showed
that certain viruses
reprogram host
cells to produce
more viruses by
injecting their DNA
Head
DNA
Tail
Tail
fiber
Mix radioactively
labeled phages with
bacteria. The phages
infect the bacterial
cells.
Phage
Agitate in a blender
to separate phages
outside the bacteria
from the cells and
their contents.
Radioactive
protein
Bacterium
Empty
protein shell
Measure the
radioactivity in
the pellet and
liquid.
Radioactivity
in liquid
Phage
DNA
DNA
Batch 1
Radioactive
protein
Centrifuge
Pellet
Batch 2
Radioactive
DNA
Campbell et al, 2003
Figure 10.1B
Radioactive
DNA
Centrifuge
Pellet
Radioactivity
in pellet
Phage attaches
to bacterial cell.
Phage injects
DNA.
Thymine (T)
Cytosine (C)
Pyrimidines
Adenine (A)
Guanine (G)
Purines
Nitrogenous
base
Sugar
Phosphate
group
Nitrogenous base
(A, G, C, or T)
Nucleotide
Thymine (T)
Sugar
(deoxyribose)
DNA nucleotide
Polynucleotide
Sugar-phosphate backbone
Phosphate
group
Uracil (U)
Sugar
(ribose)
Figure 10.2C, D
Figure 10.3A, B
G pairs with C
DNA REPLICATION
Nucleotides
Parental molecule
of DNA
DNA replication is
semiconservative
Each of the two
new daughter
molecules will
have one old
strand, derived
from the parent
molecule, and
one newly made
strand
Figure 16.10 ac
Parent cell
Conservative
model. The two
parental strands
reassociate
after acting as
templates for
new strands,
thus restoring
the parental
double helix.
Semiconservative
model. The two
strands of the
parental molecule
separate,
and each functions
as a template
for synthesis of
a new, complementary strand.
Dispersive
model. Each
strand of both
daughter molecules contains
a mixture of
old and newly
synthesized
DNA.
First
replication
Second
replication
3 end
P
P
5 end
3 end
Figure 10.5B
5 end
C
A
T
T
1 nm
Through
3.4 nm
observations of the
X-ray
crystallographic
images of DNA
A
T
A
A
T
T
C
0.34 nm
Figure 16.7a, c
Sugar
CH3
N
O
Sugar
Thymine (T)
Adenine (A)
N
Sugar
N
N
N
N
Figure 16.8
H
Guanine (G)
Sugar
Cytosine (C)
Contoh:
Diketahui bahwa kandungan Adenin dari
molekul DNA suatu makhluk hidup adalah 20%,
maka:
A = T (A = 20% ; T = 20%)
A + T = 40%
C + G = 100% - 40% = 60%,
sehingga kandungan C = 30% dan G = 30%
Nuclear; chromosomes
Nuclear genome; chromosomal DNA
Mitochondrion; Chloroplast
Extra-nuclear genome; extra-chromosomal DNA
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