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12A008R0

STAGE : FOR PUBLIC COMMENT (40.20)


DATE : 01.08.2014 30.09.2014

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DRAFT
MALAYSIAN
STANDARD

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Rice (Oryza sativa L.) hybrid seed planting


materials - Specification

ICS: 65.020.20
Descriptors: rice (oryza sativa L.), hybrid, seed, planting, materials

Copyright 2014

12A008R0

Contents
Page
Committee representation ..........................................................................................................ii
Foreword .................................................................................................................................... iii

Scope ............................................................................................................................... 1

Normative references ...................................................................................................... 1

Terms and definitions ...................................................................................................... 1

Standard procedure for seed production ......................................................................... 3

Crop field requirements ................................................................................................... 3

Seed standard.................................................................................................................. 5

Packing and labelling ....................................................................................................... 5

Inspection, sampling and testing ..................................................................................... 6

Compliance with the specifications .................................................................................. 6

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Bibliography ............................................................................................................................... 7

STANDARDS MALAYSIA 2014 - All rights reserved

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Committee representation

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Cameron Highland Flower Growers Association


Department of Agriculture Malaysia
Department of Fisheries
Department of Veterinary Services
Department of Standards Malaysia
Farmers Organization Authority
Federal Agriculture Marketing Authority
Federation of Livestock Farmers Associations of Malaysia
Fish Breeders' Association
Forest Research Institue Malaysia
Forestry Department of Peninsular Malaysia
Malaysian Agricultural Research and Development Institute
Malaysian Agrifood Corporation
Malaysian Association of Standards Users
Malaysia Fruit Exporters Association
Malaysian Palm Oil Association
Malaysian Rubber Board
Ministry of Agriculture and Agro-based Industry Malaysia
Ministry of International Trade and Industry
Ministry of Plantation Industries and Commodities Malaysia
Ministry of Science, Technology and Innovation
Rubber Industry for Smallholders Development Authority
SIRIM Berhad (Secretariat)
Universiti Putra Malaysia

The Industry Standards Committee on Agriculture (ISC A) under whose authority this Malaysian Standard was
developed, comprises representatives from the following organisations:

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The Technical Committee on Planting Materials which developed this Malaysian Standard consists of representatives
from the following organisations:

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Department of Agriculture
Malaysian Agricultural Research and Development Institute
Malaysian Rubber Board
National Seed Association Malaysia
Seng Kee Fruit Nursery
SIRIM Berhad (Secretariat)
Sin Seng Huat Seeds Sdn Bhd
Universiti Kebangsaan Malaysia
Universiti Putra Malaysia

The Working Group on Rice Planting Materials which developed this Malaysian Standard consists of representatives
from the following organisations

Department of Agriculture
Felcra Berhad
Malaysian Agricultural Research and Development Institute
Padiberas Nasional Berhad
SIRIM Berhad (Secretariat)
Co-opted members
Department of Agriculture (Paddy, Industry Crop and Floriculture Division)
Malaysian Agricultural Research and Development Institute (Rice and Industrial Crop Center)

ii

STANDARDS MALAYSIA 2014 - All rights reserved

12A008R0

Foreword
This Malaysian Standard was developed by the Working Group on Rice Planting Materials
under the authority of the Industry Standards Committee on Agriculture.

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Compliance with a Malaysian Standard does not of itself confer immunity from legal
obligations.

STANDARDS MALAYSIA 2014 - All rights reserved

iii

12A008R0

Rice (Oryza sativa L.) hybrid seed planting materials - Specification


1 Scope
This Malaysian Standard prescribes the quality requirements and sampling of hybrid rice seed
for planting.

2 Normative references

EN

The following normative references are indispensable for the application of this standard. For
dated references, only the edition cited applies. For undated references, the latest edition of
the normative reference (including any amendments) applies.
Agri Laws/Regulations (AO No. 20, series of 2005), Philippine Department of Agriculture

Hybrid Rice Breeding Manual, 1997 International Rice Research Institute Los Baos, Laguna,
Philippines

MS 469:2012, Rice (Oryza sativa L.) inbred seed planting materials Specification (Second
revision)

MS 320:2008, Seeds - Sampling and Tests (Second revision)

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3 Terms and definitions

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Standard Jabatan Pertanian 2009, Spesifikasi Pengeluaran Biji Benih Padi Hibrid (Oryza sativa)

3.1 hybrid variety

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For the purposes of this standard, the following terms and definitions apply.

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Genetically heterozygous, morphologically homogenous and distinguishable within the taxon.


3.2 rice seed

Seed of Oryza sativa L., or its derivatives produced for use as a planting material.
3.3 breeder seed
Seed of the highest genetic purity controlled and produced by the breeder or the agency that
owned the variety.
3.4 foundation seed
Seed which is the progeny of breeder or foundation seed produced under the control of the
breeder or the agency that owned the variety.

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3.5 registered seed


Seed from the progeny of breeder or foundation seed so handled as to maintain satisfactory
genetic identity and purity as designated by the local official seed certifying agency.
3.6 certified seed
Seed from the progeny of breeder or foundation or registered seed produced and handled in
such a way as to maintain satisfactory genetic identity and purity as approved by the official
seed certifying agency.
3.7 A line (CMS line)

EN

A rice line whose anthers have sterile pollen or abortive pollen. Genetic factors responsible to
induce sterility are present in its cytoplasm. No seed is set on such line by self-pollination but
its pistil is still normal and can produce seed when pollinate by any normal plant.

3.8 B line (Maintainer)

A pollinator variety used to pollinate a cytoplasmic male sterile line and produce progeny
which still remain male sterile. If there is no sterile line, the male sterile line cannot be
maintained and multiply generation after generation.

3.9 R liner (Restorer)

3.10 hybrid rice (F1)

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A pollinator variety used to pollinate the male sterile line (CMS) to produce F1 progenies
which are male fertile.

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The hybrid F1 rice breeds for commercial use. It is the product of a cross between genetically
dissimilar parents.

3.11 seed-borne diseases

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Diseases that is available in certified seed farm population which can be transmitted through
seeds.
3.12 noxious weeds
Plants other than rice plants which may contaminate the crop seed, or plants which are
difficult to eradicate once established.
3.13 off-types
Rice plants which do not conform to the varietal description offered by the breeder or the
organization which releases the variety.
3.14 certifying agency
An agency appointed by an authority body for seed certification.

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4 Standard procedure for seed production


4.1 The general requirements for hybrid rice seed production as adopted in Standard
Jabatan Pertanian 2009, Spesifikasi Pengeluaran Biji Benih Padi Hibrid (Oryza sativa) are basic
together with the succeeding specific requirements.
4.2 The production area shall have fertile soil adapted for parental lines, with sufficient
irrigation and efficient drainage system, not shaded and has no previous record of serious
incidence of weedy rice infestation, pests and diseases.

4.3 Land should be well prepared, levelled, free of weeds, volunteer seedlings and weedy
rice.

EN

4.4 Seed producers are required to first submit an application to the certifying agency to set
up a new farm seeds. Approval is subject to the status of weedy rice contamination. For
existing seed farm, producers are required to ensure that all operations have met all the
requirements in clause 4.2 and 4.3.

4.5 The production area shall undergo at least four series of inspections to be conducted by
officials from certifying agency during the seed production process at the following stages:

a) pre-planting

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b) vegetative

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c) flowering
d) pre-harvesting

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4.6 Inspection during pre-harvesting stage is to ensure that the R lines (male parent) are
harvested ahead and taken out of the field before harvesting A line (seed parent).

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4.7 During processing and storage, proper cleaning and bagging shall be observed to
maintain general cleanliness/sanitation and prevent mechanical mixtures of varieties.

5 Crop field requirements


5.1 The following field requirements and standards shall be met;
5.1.1 Isolation strictly isolated to ensure genetic purity of hybrid seeds and rice parental
and avoid pollination from unwanted varieties. The following isolation methods shall be
followed:
a) Distance isolation an isolation of 100 m is satisfactory. Within this range, no other rice
varieties should be grown except for the pollen parent;
b) Time isolation adjust planting time to separate flowering of seed parent from other
cultivars at least 3 weeks. The heading stage of varieties grown around the hybrid seed
production field should be over 21 days earlier or later than that of the cytoplasmic male
sterile (CMS) lines or A line; and

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c) Barrier isolation topographic surface features or any physical obstacles be natural or


artificial, with height of at least 2.5 m or crop barrier having a height of at least 2.2 m and
3 to 4 m wide can be used as a means of isolation barrier.
5.1.2 Each seed production shall use category designated seed from a certified source.
Category seedlings to produce seed list is basic seed. The winner of the seed to produce a
valid seed is the seed list.
5.1.3 Line ratio refers to the number of rows planted with B or R line with a line in all areas
of seed production. This ratio varies between regions and depending on the weather,
management and holding their own seeds. R line and a line can be planted at a ratio of 2:8,
2:12, 3:10 or any suitable row ratio.

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5.1.4 Roguing - sources of undesirable rice plants like volunteer plants, off-types should be
removed from A line or R line at any time of the plant stage development and more
importantly during the maximum tillering, flowering and before harvest stages; and

5.1.5 Control of weeds, diseases and insect pests keep weeds, disease and insect pests
under control.

5.1.6 Field inspection guidelines and standards recommended planting practices,


isolation, varietal field purity shall be met for the seed harvest to be eligible for certification;
the seed inspector shall certify eligibility based on the guidelines and standards for field
inspection of F1 and parental seeds production as shown in Table 1 Crop field requirements.

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Table 1. Crop field requirements

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Maximum permissible crop field requirements for


different categories of seeds (%)

Factor

Foundation
seed

Registered
seed

Certified
seed

None
None

0.05
0.05

0.1
0.1

0.3
0.3

Noxious weedsa

None

0.05

0.1

0.2

Weedy rice

None

None

0.01

0.02

Plant affected by seedbourne diseaseb

None

0.05

0.2

0.5

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Off-types
- AxB
- R Line

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Breeder
seed

Echinochloa sp., Scirpus grossus, Leptochloa chinensis, Ischaemum rugosum, Oryza


rufipogon.
b

Brown spot (Dreschlera oryzae), Bacterial leaf blight (Xanthomonas oryzae), Bacterial
leaf streak (Xanthomonas oryzicola), Bakanae (Gibberella fujikuroi), Blast (Pyricularia
oryzae) and False smut (Ustilaginoidea virens).

STANDARDS MALAYSIA 2014 - All rights reserved

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6 Seed standard
6.1 Seeds harvested from field and inspected seed crop shall undergo seed testing/analysis
and meet the seed standards as seen in Table 2. Seed requirements.
Table 2. Seed requirements
Permissible seed requirements for the different categories of
seeds
Registered
seed

99.0
1.0
None

98.0
2.0
None

98.0
2.0
None

None
None

None
None

Certified
seed

Foundation
seed

98.0
2.0
None

Germination (%;
min.)
Moisture content
(%; max.)

5 grains/kg
10 grains/kg

80

80

80

14.0

14.0

14.0

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80

2 grains/kg
5 grains/kg

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Other seeds
Noxious weed
seed (max.)
Weedy rice seed
(max.)

Physical purity (%)


Pure seed (min.)
Inert matter (max.)
Other crop seed

Breeder
seed

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Factor

14.0

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Echinochloa sp., Scirpus grossus, Leptochloa chinensis, Ischaemum rugosum, Oryza


rufipogon.

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7 Packing and labeling


7.1 Packing

Seed for certification shall be packed and sealed in suitable packages or containers to allow
for a reasonable storage period.
7.2 Labeling
7.2.1 The label of each category shall conform to the following colour:
a)
b)
c)
d)
e)

Breeder seed - orange


Foundation seed white
Registered seed (A Line) green
Registered seed (B/R Line) - purple
Certified seed - blue

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7.2.2 Each package shall be attached (sewed) with a legible and indelible label with the
following information:
a) producer name and address;
b) category
c) species
d) variety
e) parent (excluding certified seed)

lot number and

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f)

g) date of packing

7.2.3 Each package shall be provided with the following information:

a) producer name and address;

b) germination (minimum) _______%;


c) physical purity (minimum)_______%; and

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d) net weight (kg).

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7.2.4 All packages may, by prior arrangement with the certifying agency, be marked with the
certification mark of that agency.

8 Inspection, sampling and testing

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8.1 Field inspection shall be carried out in accordance with the procedure of the certifying
agency.
8.2 Sampling and tests shall be carried out in accordance with the procedure laid down in
MS 320:2008, Seeds - Sampling and Tests (Second revision).

9 Compliance with the specifications


When on testing, each of the samples is found to conform to the requirements specified in this
standard, the lot, batch or consignment from which the samples have been drawn shall be
deemed to comply with this standard.

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Bibliography
Jabatan Pertanian Malaysia (2011), Prosedur Skim Pengesahan Benih Padi

[2]

Jabatan Pertanian Malaysia (2009), Standard Jabatan Pertanian Malaysia (SJPM


2009), Spesifikasi Pengeluaran Benih Padi.

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[1]

STANDARDS MALAYSIA 2014 - All rights reserved

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Acknowledgements
Members of Technical Committee on Planting Materials
Dr Izham Ahmad (Chairman)
Ms Nor Azian Duriat (Secretary)
Mr Mohd Anim Hosnan/
Mr Fazlisyam Md Isa
Dr Mohammed Selamat Madom

Universiti Putra Malaysia


SIRIM Berhad
Department of Agriculture
Malaysian Agricultural Research and
Development Institute
Malaysian Rubber Board

Dr Masahuling Benong
Mr Wan Zuraidi Sulaiman
Mr Soo Kok Keong
Mr Freddie Soh Tan Seng
Prof Dr Normah Mohd. Noor
Prof Dr Ghizan Saleh/
Assoc Prof Dr Uma Rani Sinniah

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Seng Kee Fruit Nursery


Sin Seng Huat Seeds Sdn Bhd
Universiti Kebangsaan Malaysia
Universiti Putra Malaysia

Mr Azman Md Azhar

Co-opted members:

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Ms Siti Hadijah Hassan/


Ms Nor Azian Duriat (Secretary)
Mr Azis Yassin
Mr Mohamad Ramdzan Maulan
Mr Zainudin P M D Hussain

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Mr Mohammad Zuhairi Ali


Mr Elixon Sunian

Malaysian Agricultural Research and


Development Institute
SIRIM Berhad

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Dr Mohamad Selamat Madom (Chairman)

Members of Working Group on Rice Planting Materials

Padiberas Nasional Berhad


Department of Agriculture
Malaysian Agricultural Research and
Development Institute
Felcra Berhad

Department of Agriculture (Paddy, Industry


Crop and Floriculture Division)
Malaysian Agricultural Research and
Development Institute (Rice and Industrial
Crop Center)

STANDARDS MALAYSIA 2014 - All rights reserved

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Copyright 2014
All rights reserved. No part of this publication may be reproduced or utilised in any
form or by any means, electronic or mechanical, including photocopying and
microfilm, without permission in writing from the Department of Standards Malaysia.

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