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Abstract
The purpose of this work was to compare the sensory characteristics of ewes milk cheeses using several sensory methodologies
(free choice proling (FCP), direct similarity measurements, and hedonic data). For this purpose, visual plot inspection as well as
correlation coefcients among sensory dimensions were considered. Multidimensional maps obtained by means of free choice
proling proved difcult to interpret without any other external source of information in contrast to data obtained by direct
similarity measurement techniques that gave interpretable solutions. Cheese samples showed specic sensory characteristics mainly
based on ripening time and manufacturing procedures. The techniques used in this study revealed great individual variability during
sensory assessment of cheese samples mainly attributable to the absence of assessor training. Multidimensional solutions for the
different correlation techniques are also discussed.
r 2003 Elsevier Science Ltd. All rights reserved.
Keywords: Sensory properties; Ewes milk cheeses; Sensory methodologies
1. Introduction
Ewes milk cheeses have a unique taste and avour,
very different from that made from cows milk
(Kalantzopoulos, 1993). Furthermore, these minority
varieties have a strong quality and authenticity image
and their traditional character plays a positive role on
the consumer market (Freitas & Malcata, 2000). For
this reason, and considering a market dominated by
cows milk products, the protection of traditional ewes
milk cheeses is taking on a new signicance (Pe! rez
!
Elortondo, Ba! rcenas, Casas, Salmeron,
& Albisu,
1999b). Several research works have been recently
published on sensory characteristics within this group
of cheeses. A specic sensory lexicon and standard
references have been previously described (Lavanchy
! &
et al., 1999; Ba! rcenas, Pe! rez Elortondo, Salmeron,
Albisu, 1999). Futher studies were also carried out in the
selection of descriptive panels for these types of products
(Ba! rcenas, Pe! rez Elortondo, & Albisu, 2000; Ba! rcenas,
! & Albisu, 2001a). MorePe! rez Elortondo, Salmeron,
*Corresponding author. Tel.: +34-45-01-30-75; fax: +34-45-13-0756.
E-mail address: knppeelf@vc.ehu.es (F.J.P. Elortondo).
0958-6946/03/$ - see front matter r 2003 Elsevier Science Ltd. All rights reserved.
PII: S 0 9 5 8 - 6 9 4 6 ( 0 2 ) 0 0 1 3 9 - 5
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Table 1
Main characteristics of ewes milk cheese samples under investigation
Code
number
Cheese variety
Idiaz!abal
Idiaz!abal
Idiaz!abal
Idiaz!abal
Idiaz!abal
6
7
8
Roncal
Manchego
Castellano
10
10
7
Ripening
time
(months)
Characteristics
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2.0
DIM2
DIM1
1.5
loss
1.0
.5
0.0
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 Mean
assessors
DIM1: dimension1; DIM2: dimension 2
2.0
7
2
Dimension 2
70
0.0
.4
5
-2.0
-2.0
0.0
Dimension 1
2.0
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Table 2
Denition of bidimensional OVERALS solution for odour analysis
Dimension 1a
Dimension 2
Intensity1 (0.765)
Butyric1 (0.641)
Vegetable2 (0.500)
Intensity5 (0.753)
Vegetable8 (0.637)
Ripened11 (0.939)
Intensity11 (0.726)
Intensity12 (0.753)
Intensity13 (0.753)
Sweat13 (0.644)
Animal14 (0.918)
Intensity16 (0.570)
Intensity19 (0.596)
Moisture19 (0.753)
Intensity20 (0.637)
Cream15 (0.735)
Fresh17 (0.757)
Lactic18 (0.850)
Yoghurt20 (0.596)
Flowers4 (0.808)
Yoghurt5 (0.505)
Fresh5 (0.736)
Cream7 (0.878)
Fruity9 (0.708)
Candy9 (0.500)
Milk15 (0.500)
Sweet18 (0.739)
Sweet Cheese20 (0.660)
Concentrated milk1(0.504)
Acid1 (0.788)
Intensity4 (0.691)
Animal7 (0.691)
Sweat7 (0.568)
Intensity7 (0.568)
Acid9 (0.599)
Animal9 (0.576)
Intensity10 (0.632)
Rancid11 (0.734)
Smoke12 (0.788)
Intensity15 (0.691)
Acid17 (0.696)
Barn18 (0.788)
Numbers after each descriptor identify sensory assessor who employed it during free choice proling.
1.0
DIM2
DIM1
.8
.6
loss
.4
.2
0.0
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 Mean
assessors
DIM1: dimension1; DIM2: dimension 2
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avour and odour, respectively, indicating better consensus among panelists when evaluating avour properties. In this case, panelist number 4 showed the highest
loss (0.815), while number 6 got relatively low loss
values (0.209). This fact corroborates the hypothesis
that some individuals have greater ability to describe a
specic type of stimulus (Lesschaeve & Issanchou,
1997).
The bidimensional sample plot (Fig. 4) for avour
shows clear differences when compared with odour
sample plot (Fig. 2). There was a cluster formed by
Idiazabal cheese samples (samples 2, 4 and 5, except for
farmhouse origin, sample 1), Roncal (sample 6) and
Castellano (sample 8). Manchego (sample 7) and farmhouse Idiazabal (sample 1) cheeses were located totally
independent from the rest of the varieties. Samples were
spread especially along dimension 1, except for Manchego (sample 7) that is found at the negative end of
dimension 2.
Idiazabal cheese samples 2, 4 and 5 were located very
near to the origin (0.0) of the sample plot (Fig. 4). As
suggested by Lawless, Sheng, and Knoops (1995), this
tendency should be interpreted as a lack of clear
differences among samples involved in this clustering.
Ripened Idiazabal cheeses (samples 4 and 5) were close
together. Although (sample 5) was smoked, the assessors
Dimension 2
2.5
6.
.
4
2 .
.
5
0.0
-2.5
-2.5
.
.7
0.0
Dimension 1
2.5
3.4. Texture
The bidimensional OVERALS solution for texture
terms had eigenvalues of 0.955 and 0.872 for rst and
second dimension, respectively, giving a total tness
equal to 1.827. This value was very similar to that
obtained for avour analysis, and in any case above
odour total tness. Mean loss was 0.128, below odour
and avour values, showing a higher consensus among
Table 3
Denition of bidimensional OVERALS solution for avour analysis
Dimension 1a
Dimension 2
Intensity2 (0.797)
Salty2 (0.564)
Intensity6 (0.580)
Aftertaste8 (0,525)
Intensity9 (0.628)
Whey9 (0.723)
Pungent16 (0.807)
Bitter18 (0.736)
Sharp19 (0.644)
Acid1 (0.905)
Pungent3 (0.905)
Bitter9 (0.583)
Fresh11 (0.545)
Sweet13 (0.701)
Acid14 (0.905)
Pungent15 (0.730)
Fresh17 (0.575)
Animal17 (0.512)
Milk2 (0.507)
Intensity3 (0.665)
Salty7 (0.586)
Persistence19 (0.698)
Fresh5 (0.727)
Toasty9 (0.961)
Astringent14 (0.727)
Old15 (0.685)
Animal16 (0.587)
Dry18 (0.961)
Numbers after each descriptor identify sensory assessor who employed it during free choice proling.
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1.2
DIM2
1.0
DIM1
loss
.8
.6
.4
.2
0.0
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 Mean
assessors
DIM1: dimension1; DIM2: dimension 2
Dimension 2
2.0
7
0.0
.4 6.
5
.
1 8
. .
-2.0
-2.5
0.0
Dimension 1
2.5
73
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Table 4
Denition of bidimensional OVERALS solution for texture analysis
Dimension 1a
Dimension 2
Elasticity4 (0.591)
Pastiness6 (0.571)
Creaminess9 (0.560)
Elasticity9 (0.533)
Creaminess14 (0.955)
Adhesiveness15 (0.511)
Deformability16 (0.746)
Adhesiveness18 (0.985)
Creaminess19 (0.820)
Dry1 (0.834)
Graininess1 (0.779)
Hardness7 (0.817)
Graininess7 (0.737)
Wrinkliness10 (0.533)
Hardness12 (0.591)
Greasy12 (0.986)
Greasy13 (0.985)
Graininess13 (0.511)
Plastic14 (0.994)
Hardness15 (0.570)
Hardness16 (0.841)
Hardness17 (0.985)
Greasy17 (0.902)
Greasy20 (0.718)
Graininess20 (0.985)
Deformability1 (0.750)
Friability2 (0.676)
Firmness4 (0.516)
Elasticity5 (0.705)
Friability10 (0.509)
Wrinkliness11 (0.571)
Creaminess12 (0.628)
Adhesiveness15 (0.703)
Pastiness16 (0.783)
Creaminess20 (0.789)
Hardness2 (0.774)
Deformability2 (0.548)
Hardness3 (0.750)
Hardness5 (0.753)
Pastiness6 (0.563)
Wrinkliness8 (0.516)
Greasy8 (0.682)
Hardness11 (0.572)
Crunchy12 (0.628)
Graininess18 (0.687)
Wrinkliness20 (0.605)
Numbers after each descriptor identify sensory assessor who employed it during free choice proling.
.6
2
1 4
10
.5
Dimension 2
15
11
17 5
13
.4
12
.3
16
14
6
9
18
3
.2
.2
.3
.4
.5
.6
.7
Dimension 1
Fig. 7. INDSCAL analysis. Dimensional weights for assessors evaluating ewes milk cheeses using direct similarity measures.
2.0
. .4
5
Dimension 2
.7
2
.
. 3.
0.0
-2.0
-2.0
0.0
2.0
Dimension 1
Fig. 8. INDSCAL analysis. Multidimensional arrangement of cheese
samples based on individual direct similarity data matrices. Details on
cheese samples can be found in Table 1.
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Table 5
Two-way ANOVA (sample assessor) and Taguchi signal-to-noise ratio (SNR) for hedonic measures
Source
dF
SS
Sign.
Samples
Assessors
Total error
Samplesa
Hedonic score
Taguchi SNR
6
19
139
7
4.6
12.35
19.486
113.029
363.886
5
4.4
11.56
3.248
5.949
0.153
0.000
8
4.1
10.49
4
3.9
10.51
1
3.6
9.01
2
3.4
8.94
1.5
7
Dimension 2
6
4.0
10.12
2
1
0.0
-1.5
-1.5
0.0
1.5
Dimension 1
Fig. 9. Bidimensional MDPREF biplot showing cheese samples
(triangles) and consumers (dots). Details on cheese samples can be
found in Table 1.
4. Conclusions
The FCP has proved to be successful in the sensory
description of several food products. However, in this
study the maps obtained by means of this technique
were not easy to interpret without any other external
source of information (e.g. traditional sensory prole,
familiarity with cheeses, etc.). Manchego cheese variety
showed the highest sensory differences among samples,
these differences being the most easily interpretable
ones. Cheese samples showed specic sensory characteristics mainly based on ripening time, although this
evidence may be considered tenuous mainly due to the
lack of panel consensus during attribute scoring. Great
individual variability was observed for the considered
techniques. These problems could have been avoided by
the use of a trained panel.
The main dimensions showed signicant correlation
for almost every multidimensional solution. These
results described the similarities between the underlying
dimensions once assessors are evaluating the samples.
Therefore, it can be concluded that the sensory test to be
employed should be carefully selected and, if possible,
validated with allied methods depending on the
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Table 6
Correlation matrix for sample scores obtained by the different sensory methodologies
1
2
3
4
5
6
7
8
9
10
INDS1
INDS2
ODOR1
ODOR2
FLAV1
FLAV2
TEXT1
TEXT2
MDPREF1
MDPREF2
10
1.000
0.044
0.747b
0.552
0.791b
0.431
0.546
0.148
0.823b
0.247
1.000
0.509
0.509
0.235
0.015
0.221
0.096
0.237
0.239
1.000
0.001
0.635
0.231
0.493
0.312
0.649
0.139
1.000
0.382
0.482
0.357
0.255
0.588
0.435
1.000
0.001
0.159
0.157
0.443
0.004
1.000
0.937c
0.051
0.429
0.642
1.000
0.000
0.473
0.613
1.000
0.435
0.541
1.000
0.000
1.000
INDS: Direct similarity measures; ODOR: odour free choice proling; FLAV: avour free choice proling; TEXT: texture free choice proling;
MDPREF: preference measures; 1: dimension 1; 2: dimension 2.
b
po0:05:
c
po0:01:
Acknowledgements
This work was nanced by Universidad del Pa!s
Vasco (UPV 101.123-TA095/96). Pedro Ba! rcenas
thanks the Departamento de Industria, Agricultura y
Pesca of the Basque Government for a fellowship.
Members of the ewes milk cheese panel are thanked for
their enthusiastic participation in this study.
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