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Cold Trub:

Implications for Finished Beer, and Methods of Removal


By Ron Barchet
Republished from BrewingTechniques' March/April 1994.
In the November/ December 1993 issue of BrewingTechniques, Ron Barchet described the
importance of removing hot trub. In this issue, he discusses cold trub and its implications for
brewers. Although brewers' opinions vary as to cold trub's ultimate impact on finished beer,
certain facts are generally agreed upon. The presentation in this article is descriptive, not
prescriptive.
Cold trub consists of proteins, protein-polyphenol complexes, and carbohydrates (Table I). The
protein component of cold trub consists of by-products from the breakdown of hordein
(prolamin) and globulin. These by-products are soluble in hot wort but precipitate as the wort
cools. Approximately 15-25% of these proteins bind to polyphenols, forming protein-polyphenol
complexes. Higher molecular weight carbohydrates, called beta-glucans, make up 20-30% of
cold trub (2).
As Table I shows, the composition of cold trub produced varies with the percent difference
between fine and coarse grist extract and the degree of malt modification. Highly modified malts,
for example, yield a higher percentage of polyphenols in cold trub than do less-modified malts.
Undermodified malts yield more protein and relatively fewer polyphenols (3).
Cold trub proteins and polyphenols begin to bind at lower temperatures, especially when cooled
to <170 F. Consequently, as wort cools, cold trub precipitates (see Table II). If conditioned beer
or wort is reheated, cold trub will go back into solution. Chill haze in finished beer is, essentially,
cold trub. Most beers that exhibit chill haze clarify as they are brought to room temperature (the
cold trub goes back into solution).

FACTORS AFFECTING QUANTITY OF COLD TRUB PRODUCED


The total dry weight of cold trub is 15-30 g/hL (17-35 g/bbl); the actual amount produced in a
given wort depends on numerous factors such as malt modification, mashing program, wort
temperature, and the presence of hops or adjuncts (3).
Undermodified malts yield fewer polyphenols and more beta-glucans (Table I). Wort produced
using finely milled malt contains a large quantity of cold trub because the finely ground husks
allow greater polyphenol extraction. On the other hand, more-intensive mashing programs, such
as double and triple decoction, degrade proteins more extensively and yield less cold trub than do
infusion brews, in which more high molecular weight proteins pass through to the chilled wort
(Table III).
Boiling the wort reduces the quantity of cold trub, yet adding hops, which contain polyphenols,
increases cold trub. Hops and hop products that contain or contribute fewer polyphenols yield
less cold trub (4). Furthermore, adding hops late in the boil increases the amount of cold trub

formed. Cold trub precipitates best between 41 and 32 F (5). Thus, to minimize cold trub in a
hop accented beer, one should have a lagering capability of 29-32 F.
Finally, worts brewed with adjuncts produce proportionately less cold trub, whereas higher
gravity worts produce more cold trub.

REMOVING COLD TRUB: HOW IMPORTANT?


Opinions vary as to the exact effects of removing cold trub. The way cold trub removal affects
beer depends on yeast strain, the number of yeast generations used, the method and amount of
removal, and the overall brewery characteristics.
It is widely believed that removing all cold trub not only has no benefit, but actually might slow
fermentation and harm the finished beer, reportedly giving it an onion-like flavor. Stroh Brewing
Co. reported slower fermentation, higher acetate ester levels, and lower yeast growth and
viability after removing all cold trub from test batches (6). Further experiments showed another
effect of the complete elimination of trub: the absence of nucleation sites during fermentation
resulted in a supersaturation of carbon dioxide in the wort; high levels dissolved carbon dioxide
inhibit fermentation. Stroh's work revealed the importance of having at least some wort solids
present to act as carbon dioxide nucleation sites.
Removing at least some cold trub, however, has been shown to improve yeast viability and the
quality of finished beers (3). Studies performed in Germany (where wort and beer are all-malt)
have shown that partial removal of cold trub is beneficial to the stability of packaged beer.
Flotation accomplishes this by at least partially removing proteins, polyphenols, carbohydrates
and heavy metals, such as copper ions and iron complexes, which can catalyze oxidation
reactions (7). Taste tests have also shown a preference for beers that were brewed with yeast
harvested from beer from which trub had been removed by flotation.
Yeast growth and fermentation in high-gravity worts may benefit from leaving cold trub in the
wort, which will lower the carbon dioxide in the fermenting wort, thus repressing the carbon
dioxide's inhibitory effect on fermentation.
The cost of extra equipment and labor and the increased risk of contamination are valid
arguments for not removing cold trub.

METHODS OF COLD TRUB REMOVAL


Settling tank: The simplest method of removing cold trub, and one used by most home brewers,
is the settling tank. Because hot trub also settles, settling tanks can be used for removing both hot
and cold trub.
Settling tanks can be used with pitched or unpitched wort. If your lag time to fermentation is <12
h, it is best to remove the cold trub before pitching. For longer lag times, you can pitch first and
remove the trub just before active fermentation begins.

Removing cold trub from pitched wort. Move chilled, aerated and pitched wort to a tank, allow it
to settle for 12-16 h, and then rack to another tank, leaving the sediment behind. Racking should
be done before the active phase of fermentation begins because the natural circulation of carbon
dioxide in fermentation will rouse the settled trub. Yeast strains that are highly flocculent may
also settle during the lag phase and become mixed with trub. If using such a strain, overpitch by
the amount that settles out in the cold trub. As much as 30% of the total cold trub settles out of
pitched wort after a 12-h rest (5).
Removing cold trub from unpitched wort. Again, the wort stands for 12-16 h. It is then moved to
another vessel, aerated, and pitched with yeast. Sedimentation of unpitched worts is more
complete than that of pitched worts (~50%) (5).
Because wild yeast and bacteria are a concern and must be avoided, care must be taken to ensure
that the settling tank is sanitary, particularly if the wort is unpitched. Infection will not only
affect the finished beer but contaminate the yeast as well.
Shallow vessels rather than cylindroconical tanks make the best settling tanks. Deeper vessels
require more time but can benefit from an addition of 10-20 g/hL (12-24 g/bbl) coarse
diatomaceous earth. As much as 70% of total cold trub precipitates when using diatomaceous
earth (3).
Flotation: Flotation is becoming more common as a method of removing cold trub. In flotation,
the cooled wort is saturated with sterile air. As the air bubbles make their way to the top of the
tank, they carry cold trub with them. After 2-3 h, a brown, compact head forms at the top of the
wort. Wort is then removed from the bottom of the tank, leaving the cold trub behind. Even more
cold trub (50-65% of total cold trub) can be removed if wort stands 6-8 h before being racked
(3). Brewery characteristics will determine optimal standing time.
It is important not to allow the cold trub cake to break and fall into the clean wort, which will
occur if the wort stands too long or if transfer is not halted before the cake is broken. In-line sight
glasses are helpful in judging when to stop transfer. Quickly cooling wort and aerating with
turbulence will promote good flocculation of cold trub, making it easier to remove before
fermentation (3).
The amount of air needed for flotation is ~40-60 L/hL (47-70 L/bbl), depending on bubble size
(2). Air is usually injected through a ceramic or metal carbonating stone (pore size ~5 m) (3)
on the cold side of the heat exchanger.
Flotation can be performed with or without yeast. The presence of yeast does not compromise
the capability of flotation to remove cold trub. In fact, yeast benefits from the oxygen in sterile
air, and only a small amount of yeast becomes trapped in the trub cover. The positive effects of
flotation on fermentation outweigh any loss of yeast trapped during the process.
For effective flotation, leave at least 30-50% headspace to accommodate the foaming that occurs
at the beginning of the process; for example, wort should stand no higher than 13 ft in an 18-ft
tank. Flotation is faster in shallow vessels. Conversely, cylindroconical tanks are too tall, do not

disperse air uniformly, and cause the trub cake to break and enter the clear wort as it approaches
the cone (3). On the other hand, the strong convection currents of cylindroconical fermentations
pull polyphenols down and away from oxidation worries. If using cylindroconical fermentors, it
is best to remove yeast and cold trub regularly. Injecting pure oxygen (rather than sterile air) in
the quantity necessary to create the flotation effect will overoxygenate the wort.
Table IV compares the relative effectiveness of settling tank and flotation methods.
Other methods: Other methods of cold trub removal include centrifugation and diatomaceous
earth filtration. A centrifuge used to remove hot break can also be used to eliminate cold break.
However, because particle size is smaller and viscosity is higher in cold wort, the plate spacing
and angular velocity must be adjusted. Approximately 50-60% of total cold trub can be removed
using centrifugation (3).
Diatomaceous earth filtration is the most effective means of removing cold trub. Typically,
coarse diatomaceous earth is used, and the best results occur at low temperatures. On average,
diatomaceous earth filtration will remove 75-85% of total cold trub. Higher rates (95%) of
removal are possible with fine diatomaceous earth and 32 F wort (3). Diatomaceous earth
filtration risks removing too much cold trub, resulting in poor yeast growth and fermentation.
Wort must be fully aerated after it is centrifuged or filtered with diatomaceous earth.

CONCLUSION
Although some controversy surrounds this subject, I believe that removing some cold trub, like
removing hot trub, might provide benefits to the finished beer from certain breweries. Unlike hot
trub removal, however, complete removal of cold trub is neither possible nor desirable. The
effects of cold trub removal are subtle and may not be noticeable for several generations. It may
be detrimental to some beers, especially those made from high-gravity worts. Cold trub removal
might promote quick, clean fermentations by enhancing yeast viability. It also can extend the
shelf life of beer by removing oxidation catalysts and haze-forming constituents.
Most American breweries no longer remove cold trub. Some European breweries, notably
German, do practice some form of cold trub removal, usually flotation. It is obvious that brewers'
opinions vary, a fact that probably reflects the subtle nature of the effects of cold trub removal as
well as the dynamics of each brewery and its ingredients.
As to which cold trub removal procedure is best, it is difficult to generalize. I recommend trying
different methods on various beers and noting the differences in the resulting beer and in future
generations of beer fermented with yeast harvested from worts from which cold trub has been
removed. Pale, fine beers such as pilsners are more likely to showcase improvements than are
robust, darker styles.

REFERENCES
(1) L. Narziss and K. Bauer, Brauwissenschaft 28 (1975).

(2) L. Narziss, Abribeta der Bierbrauerei, p. 188 (1986).


(3) L. Narziss, Die Bierbrauerei: Die Technologie der Wrzebereitung, 321 (1985).
(4) L. Narziss, E. Reicheneder, and H. Hamacher, Brauwelt 109, 773 (1969).
(5) L. Narziss and H. Miedaner, Brauwelt 107, 49 (1967).
(6) K.J. Siebert et al., MBAA Technical Quarterly 23, 37-43 (1986).
(7) J.S. Hough, D.E. Briggs, R. Stevens, and T.W. Young, "Hopped Wort and Beer," Malting and
Brewing Science, p. 822 (1982).