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Kristine Bernadette Galvez, Nilufar Haghani Rad, Dixie Mae Lacap, Justyn
Ellin Michaella Laus, Patrick Daniel Libiran
ABSTRACT
Isolation of RNA from yeast, assessment of the purity of the extracted RNA
and lastly the characterization of RNA following basic hydrolysis were the
major objectives of the study. The yeast or scientifically termed as
Saccharomyces cerevisiae was used to isolate RNA. A portion of the
extracted RNA was subjected to alkaline hydrolysis using 0.3 M NaOH. The
RNA hydrolysate was the characterize by four different tests namely test for
ribose, test for phosphate, test for purines or Murexide Test and lastly test for
pyrimidines or Wheeler-Johnson Test. A dark brown solution, yellow
crystalline precipitate, red residue, and a turbid white solution with acidic pH
were obtained from each test respectively. The results from the reaction of
the RNA hydrolysate towards the different tests were compared to the
positive results obtained from the standard RNA sample. Standards served as
the basis whether the extracted RNA sample gave expected results.
INTRODUCTION
Nucleic acids are the main
information carrying molecules of
the cell, they allow organisms to
transfer genetic information from
one generation to the next by
directing the process of protein
synthesis. There are two main
structural classes of nucleic acids
namely deoxyribonucleic acid or
DNA and ribonucleic acid or RNA
which are basically made up of
nitrogenous bases derived from
purines which are adenine (A) and
guanine and from pyrimidines
which are cytosine (C) and uracil
(U), sugar, and phosphate group.
DNA is the master blueprint
for life and constitutes the genetic
RNA
from
Yeast
darkbrown
solution
yellow
precipitat
e
red
residue
turbid
white
solution
~ acidic
Standard
Solution
dark-blue
solution
yellow
solution
yellow
residue
violet
precipitat
e
~ basic
DISCUSSION
In the test for the presence of
ribose, the orcin reaction was used.
The conversion of ribose to an
aromatic aldehyde which then
reacts with Orcin to form an
aldehyde-phenol
condensation
product that in a theoretical result
is blue-green/dark green in color,
this reaction depends on. The
result we had was a dark brown not
a dark green or blue-green
solution. The failure in getting a
positive in the RNA of yeast was