Haibo Wang

112 Chestone Court Phone: (919) 448-1589

Cary, NC 27513 E-mail: angelatonger@yahoo.com
SUMMARY and QUALIFICATIONS
• Over 5 years of research experience in the field of obesity, diabetes and aging related
macular degeneration (AMD).
• Extensive research experience in the primary adipocytes, human endothelial cells and
epithelial cells.
• Possessing a variety of knowledge and techniques from molecular biology to cell
biology, immunology and pharmacology.
• Remarkable capability to manage multiple projects at the same time and good at
adjusting to changing priorities.

• Strong background knowledge about G-protein Coupled receptors such as ß-adrenergic

receptors and Muscarinic Cholinergic Receptors, and transcription factors such as
peroxisome proliferator activated receptors (PPARs) and liver X receptors (LXRs).

• Broad experience in conducting and supervising study, protocol development, and data
analysis/reporting

• Basic knowledge of GLP guidelines.

BIOTECHNOLOGY SKILLS
Western blotting, ELISA, Immunohistochemistry, enzymatic activity assays, cell
differentiation and migration assay, cell culture (cell lines and primary cell cultures),
primary mouse adipcotyes and hepatocytes isolation, human retinal pigment epithelium
and choroidal endothelial cells isolation, DNA/RNA/protein extractions, ChIP Assay
(chromatin immunoprecipitation), DNA cloning, DNA plasmids tranfection, gene silencing
by siRNA, PCR/qPCR, protein purification, ATP assay, GSH/GSSG assay, apoptosis
assay, protein phosphorylation assay, and animals handling

COMPUTER SKILLS
MS-Word, Excel, PowerPoint, Access; gene expression data analysis and interpretation

PROFESSIONAL EXPERIENCE

May, 2009 – present Postdoctoral Research Associate, Department of Ophthalmology,

University of North Carolina at Chapel Hill, Chapel Hill, NC.

Biomedical research in the field of aging related macular degeneration (AMD),

diabetic retinopathy and prematurity of retinopathy

• Led a NIH funded project to study vascular endothelium growth factors (VEGFs) in the
mediation of choroidal endothelial cells (CECs) transmigration in Neovascular Age-
related macular Degeneration using human eye samples;
 • Developed the therapeutic targets for preventing and curing aging related macular
degeneration (AMD);

• Conducted mouse laser injury choroid neovascularization (CNV) model

• Tested the effect of an inhibitor of CCR3 in CECs migration (a project collaborating with
Biotech company)

August, 2004 – May, 2009 Postdoctoral Fellow, The Hamner Insitutes for Health
Sicences, RTP, NC

Biomedical research in the field of obesity and diabetes

• Led a project to identify the role of liver x receptors (LXRs) in the regulation of
uncoupling protein-1 dependent energy expenditure (thermogenesis) in rodents;
• Designed, executed and provided valuable in vitro and in vivo data to identify the
molecular mechanism of LXRα in the suppression of energy expenditure;
• Contributed to human adipcotyes investigative team: designed and developed assays to
detect mitochondrial mass, oxygen consumption and lipolysis assay;
• Conducted gene expression assay and reporter gene activity assay using real time PCR,
gene transfection and ELISA;
• Performed Chromatin immunoprecipitation assay to analyze transcriptional factors DNA
binding activity;
• Conducted glucose tolerance test (GTT) and insulin tolerance test (ITT) in mice
• Developed obese and atherosclerosis models in mice

July, 1998 – August, 2004 Instructor and assistant professor, China Medical
University, Shenyang, P.R.China

Colorectal cancer related and Anti- Allograft Rejection Drug Development

• Led Nature Science Foundation of China project to Screen agonists and antagonists
of muscarinic achetylcholine receptors (mAChRs) using mAChRs-G protein fusion
protein;
• Led Nature Science Foundation of China project to develop a comanti-acute allograft
rejection Compared the efficacy of Chinese herbs, hyperbaric oxygen (HBO) and anti-
rejection drug cyclosporine A on the effect of in mice with skin or bone marrow
transplantation;
• Conducted and supervised study, protocol development, and data analysis/reporting;
• Interviewed and screened subjects (patients);
• Wrote the studying reports and papers ;
• Prepared research proposal and protocol for grant application;
EDUCATION

2004 Ph.D in Pathology and Pathphysiology China Medical University, Shenyang, China

1998 M.S. in Pathology and Pathphysiology China Medical University, Shenyang, China
1995 MD in Clinical Medicine China Medical University, Shenyang, China

AWARDS AND HONORS

• Fellow/Student Associate Member of The endocrine Society (Member ID#: 233016)
• Full Member of ARVO, The Association of Research in Vision and Ophthalmology
(MEMBER ID: 224138)
• Completing Good Laboratory Practices Course Chaired by Thomas Hughes of the
U.S.EPA sponsored by the EPA-RTP Trainee Organization NTO), 2006
• Postdoctoral Fellowship Award sponsored by American Heart Association (AHA),
2009
• Science and Technology Award Sponsored by Institute of Science and
Technology of Liaoning Province, 2003.
• Grant Titled as The Study on some drugs specific for mAChRs (m1-m5) using
mAChRsusion Protein funded by Nature Science Foundation of Liaoning Province
(002039) ( Co-Investigator), 2000-2002
• Grant Titled as The Expression and Function of mAChRs Fusion Protein funded by
Nature Science Foundation of China (30171077) (Co-investigator), 2002-2004
• Scholarship of Medicine Sponsored by International Exchange Center of National
Health Institute of China and the First Pharmaceuticals of Japan, 1998

PEER REVIEWED PUBLICATIONS and PRESENTATIONS

• Haibo Wang, Pete Geisen, Erika Wittchen, Keith Burridg, Mary Elizabeth Hartnett. THE
ROLE OF RPE CELL ASSOCIATED VEGF189 IN CHORIODAL EC TRANSMIGRATION
IN NEOVASCULAR AGE-RELATED MACULAR DEGENERATION. (In preparation)
• Haibo Wang and Sheila Collins. Cyclic AMP-dependent phosphorylation of liver X
receptor-α in brown adipocytes permits recruitment of RIP-140 for transcriptional
repression of the uncoupling protein-1 gene (In preparation)
• Naresh Kumar, Haibo Wang*, Dianxin Liu, Sheila Collins. Liver X receptor is a regulator
of orphan nuclear receptor NOR-1 gene transcription in adipocytes. International Journal
of Obesity, 2009 May; 33(5):519-24. (* equal contribution with the first author).
• Kumar N, Liu D, Wang H*, Robidoux J, Collins S. Orphan Nuclear Receptor NOR-1
Enhances cAMP-Dependent Uncoupling Protein-1 Gene Transcription. Molecular
Endocrinology, 2008 May; 22(5):1057-64. .
• Haibo Wang, Yuan Zhang, Einav Shnaidman, Alexander V. Medvedev, Naresh Kumar,
 Kiefer W. Daniel, Jacques Robidoux, Micheal Czech, David J Mangelsdorf, Sheila
Collins. LXRα IS A TRANSCRIPTIONA EPRESSOR OF UNCOUPLING PROTEIN-1
AND THE BROWN FAT PHENOTYPE. Mol Cell Biol. 2008 Apr; 28(7):2187-200.
• Qin Hao1, Jacob B. Hansen, Rasmus K. Petersen, Philip Hallenborg, Claus
Jørgensen, Saverio Cinti, Philip J. Larsen, Knut R. Steffensen, Haibo Wang, Sheila
Collins, Jun Wang, Jan-Åke Gustafsson, Lise Madsen, and Karsten Kristiansen.
ADD1/SREBP1c activates the PGC1<alpha> promoter in brown adipocytes.
Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 2009 (In press)
• WANG Hai-bo, QIN Xing-jun, GUO Zheng-dong, YANG Jun-lan. m5AChR-G11 fusion
protein as a tool for screening m5 ligand. Journal of China Medical University, 2005; 34
(5): 396-398
• Wang, haibo, Guo Zhengdong, Zhang Haipeng, et al. The preparation, expression and
effect of ligands on the function of m4AChR-Gila fusion protein. Chinese Journal of
Biochemical Pharmaceutics, 2004; 25 (5): 257-260
• Guo Zheng-dong, WANG Hai-bo, LI Zhi, LIU Hong-Rui, Guo Zheng-Li, ZHAO Li-Bin.
Effects of various muscarinic ligands on the expression and function of m5 muscarinic
acetylcholine receptor-G11 fusion protein. Chinese Journal of Pharmacology and
Toxicology, 2004, 18 (1):11-16
• Wang, Haibo, Guo Zheng-dong, Zhang Hai-peng, et al. Preparation of m1AChR-G11
fusion protein and effects of various ligands on them. Chinese Journal of
Pathophysiology 2003, 19 (12):1590-1595
• Guo Zhengdong, Wang, Haibo, Li Zhi, et al. Effects of various muscarinic ligands on the
expression and function of m1AChR-G11 fusion protein. Chinese Pharmacological
Bulletin, 2003; 19 (11):1294-1298
• Guo Zhengdong, Wang Haibo, Liu Hongri, et al. The Changes of the Properties of
Muscarinic Cholinergic Receptors in Smooth Muscles of Human Colorectal
Neoplasm. Chinese Pharmacological Bulletin, 2003; 19(2): 225~226
• Wang, Haibo, Guo Zhengdong, Tian Wei, et al. The protective effects of Astragale
on the cerebral ischemia-reperfusion injury in rats. Chinese New Medicine, 2003; 4
(12): 1059-1060
• Zhao Hong, Wang, Haibo, Zhang Haipeng, et al. The Effects of Hyperbaric Oxygen and
Chinese Herbal Medicine on the Expression of Spleen T-Lymphocyte and Adhesion
Molecular after Skin Transplantation in Mice. The Journal of Chinese Nautical Medicine,
2000; 17 (1)
• Wang, Haibo, Zhao Hong, Zhang Haipeng, et al. The Study of Effects of Hyperbaric
Oxygen on T -lymphocyte and Cell Adhesion Molecular after Skin Transplantation in
Mice. The Journal of China Pathophysiology, 1999; 15 (12): 1097~1099

• Haibo Wang, Kiefer W. Daniel, YS Jeong*, Y Zhang, DJ. Mangelsdorf*, S Collins. LXR
alpha Antagonizes the β-adrenergic Increase in Uncoupling Protein-1 Gene
Transcription and Mitochondriogenesis in Adipocytes. Keystone Symposia 2007.
• Haibo Wang, Yuan Zhang*, Einav Yehuda-Shnaidman, Naresh Kumar, Kiefer W.
Daniel, Jacques Robidoux, Micheal P. Czech#, DJ. Mangelsdorf*, Collins S. Liver X
Receptor alpha is a transcriptional repressor of uncoupling protein-1 gene and brown fat
phenotype. Communicative Meeting between The Hamner Institutes for Health Sciences
and The UNC Department of Cell and Molecular Physiology April 2008

• 66th American Diabetes Association Meeting, Washington DC, June 9-12, 2006. “Liver X
Receptor Alpha Antagonizes the Cyclic AMP Dependent Uncoupling Protein-1 Gene
Transcription in Adipocytes”

• Chinese Association of Pathophysiology (CPA) meeting, Shanghai, P.R.China,

December, 2003. “The Expression and Function of Fusion protein m2AChR-Gilα”

• Conference on Inflammation, Fever, Infectious Hypothermal, Chinese Association of

Pathophysiology (CPA), Dali, P.R.China, April, 1996 “Hyperbaric Oxygen Inhibits
Rejection after Skin Transplantation in Mice.