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Microfluidic Bioassays
Miguel Berenguel1*, Xavier Granados2, Jordi Faraudo2, Julin Alonso1 and Mar Puyol1
Sensors & Biosensors Group, Universitat Autnoma de Barcelona, Bellaterra 08193 (Spain)
2
Institut de Cincia dels Materials de Barcelona (ICMAB-CSIC), Campus UAB, Bellaterra 08193 (Spain)
* Miguel.Berenguel@uab.cat
1
G B
Grup de
Sensors i
Biosensors
Introduction
Research Goals
Design and study of a magnetic actuator to control the MBs and
enhance the kinetics of a reaction by actively mixing on-chip
Thus, Configuration B was chosen for the experimental setup, as it fulfills the criteria of simplicity,
magnetic field strength (saturation of the MBs) and appropiate size.
Results
The microfluidic device is fabricated using the thermoplastic Cyclic Olefin Co-polymer (COC). Its design is very simple for this first
stage of the research. It consists of two main microfluidic elements, namely the reaction chamber, to carry out the reactions of
the bioassay, and the optical detection chamber, for the fluorescence measurements. The fabrication process is shown in Fig. 1.
Off-Chip
On-Chip
Magnetic Actuator
A
Detector
Rotation Axis
The reaction product was measured with a miniaturized fluorescence detection system (see Fig. 5).
The movement of MBs can be tuned by changing the rotation speed of the magnetic actuator, and it
enhances the reaction kinetics due to the mixing. The optimal movement velocity was found to be 1.7
mm/s (see Fig. 6). The calibration curves show a 2.7-fold sensitivity enhancement (see Fig. 7) when
using the magnetic actuator. Concentrations of E. Coli as low as 600 cfu/mL have been determined.
Optical Filter
Magnetic
Beads
Insertion Port
Rotation
Axis
Microfluidc Chip
LED
Magnets
D
0
90
180
270
90
180
Figure 6. Signal obtained at different movement velocities of the MBs. The dashed line
indicates the relative signal at 0 mm/s.
Figure 7. Calibration curves for the immunoassay performed without mixing (0 mm/s) and
at two different mixing speeds.
270
Different concentrations of E. Coli O157 have been determined using a fluorescence immunoassay.
The magnetic actuator showed a 2.7-fold sensitivity enhancement over the retention-only systems.
Further research must address the integration of all the immunoassays reactions on-chip.
Acknowledgements
The authors acknowledge the financial support of the Government of Catalonia (FI program), MICINN (project CTQ2012-36165),
co-funded by FEDER, and MEC (Consolider Nanoselect, MAT200801022).