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https://sites.google.com/site/proteintransportandsecretion/protocols-2/biochemical-protocols/common-solutions[31/03/2015 13:42:20]
NP40
TRANSFER BUFFER 25 mM Trisbase, 192 mMglicina,20%
metanolo Sciogliere 3,03 gr di Tris a 14,4 gr di glicina in circa
750 ml di H20 portare a pH 8,3; aggiungere 200 ml di metanolo
a portare a volume finale di 1 1
TRISGLICINA (10X) per RUNNING BUFFER SDSPAGE 144g
GLICINA, 30g TRIS BASE bring to 1L con H2O or 576g GLICINA,
120g TRIS BASE bring to 4L with H2O
TRISHC1 pH 6.8 (0.5M): Dissolve 6 g Tris in 40 ml H2O,
adjust pH with 48 ml HCl 1Mto 100 ml final with H2O.
TRISHCI IM pH 7.5: dissolve 121.1 g TRIS base in H2O.
Adjust pH with HCI (65ml pH 7.5) bring to 1L.
TRISHCL pH 8.8 (3M): Dissolver 36.3 g tris in 40 ml di H2O,
adjust pHwith 48 ml HCI 1Mbring to100 mlwith H2O
UPPER GEL BUFFER 4X 0, 5 M TrisHCI, 0, 4 % SDS, pH 6, 8.
Add30,3g Trisbase, 10 ml SDS 20% pH 6.8 with HCI add
H2Oto 500 ml final
LOWER GEL BUFFER 4X 1,5 M TrsHC1, 0,4 % SDS, a pH 8,8.
Add90,9g Trisbase, 10 ml SDS 20%pH 8.8with HCIadd H2O
to 500 ml final
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https://sites.google.com/site/proteintransportandsecretion/protocols-2/biochemical-protocols/common-solutions[31/03/2015 13:42:20]